Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 882 254.4 12957588 98.0% 12698436 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,142,034 A→G Q154R (CAG→CGG)  pflD → formate acetyltransferase 2

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,142,0340AG95.2% 579.9 / 15.4 190Q154R (CAG→CGG) pflDformate acetyltransferase 2
Reads supporting (aligned to +/- strand):  ref base A (2/7);  new base G (77/103);  total (79/111)
Fisher's exact test for biased strand distribution p-value = 3.08e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.48e-02

Only 100 of 202 total aligned reads displayed.

TGAAAGATTTCATCAACGGGCAGATGACAGATGAAGTAAAAGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGACGGATAAAGGCCAGGGGCACATTATTATTGATTACCCACGCCTGTTGAATCACGGGCTGGGTGAGCTGGTGGCACAGATGCAGCAACATTGTCAGCAACAGCCG  >  NC_000913/4141944‑4142125
                                                                                          |                                                                                           
 gAAAGCTTTCATCAACGGGCAGATCACAGATGAAGTAAAAGCCGCTACCAACACGCAGATTTTCAGCATCACCCAGACGGATAAAGGCCgggg                                                                                          <  2:4712309/93‑1 (MQ=255)
 gAAAGATTTCATCAACGGGCAGATGACAGATGAAGTAAAAGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGACGGATAAAGGCCgggg                                                                                          <  1:6411441/93‑1 (MQ=255)
 gAAAGATTTCATCAACGGGCAGATGACAGATGAAGTAAAAGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGACGGATAAAGGCCgggg                                                                                          >  1:5744865/1‑93 (MQ=255)
  aaaGATTTCATCAACGGGCAGATGACAGATGAAGTAAAAGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGACGGATAAAGGCCGgggg                                                                                         <  2:105930/93‑1 (MQ=255)
  aaaGATTTCATCAACGGGCAGATGACAGATGAAGTAAAAGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGACGGATAAAGGCCGgggg                                                                                         >  1:5579338/1‑93 (MQ=255)
    aGATTTCATCAACGGGCAGATGACAGATGAAGTAAAAGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGACGGATAAAGGCCAGGGGca                                                                                       <  1:3706785/93‑1 (MQ=255)
     gATTTCATCAACGGGCAGATGACAGATGAAGTAAAAGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGACGGATAAAGGCCGGGGGcac                                                                                      >  1:1433328/1‑93 (MQ=255)
     aaTTCCACCAACGGGCAGATGACAGATGAAGTAAAAGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGACGGATAAAGGCCGGGGGcac                                                                                      <  2:6191056/92‑1 (MQ=255)
      aTTTCATCAACGGGCAGATGACAGATGAAGTAAAAGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGACGGATAAAGGCCGGGGGcaca                                                                                     <  1:3522702/93‑1 (MQ=255)
      aTTTCATCAACGGGCAGATGACAGATGAAGTAAAAGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGACGGATAAAGGCCGGGGGcaca                                                                                     >  2:3786098/1‑93 (MQ=255)
      aTTTCATCAACGGGCAGATGACAGATGAAGTAAAAGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGACGGATAAAGGCCGGGGGcaca                                                                                     <  1:3864853/93‑1 (MQ=255)
       tttCATCAACGGGCAGATGACAGATGAAGTAAAAGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGACGGATAAAGGCCGGGGGCACat                                                                                    <  1:2041496/93‑1 (MQ=255)
        ttCATCAACGGGCAGATGACAGATGAAGTAAAAGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGACGGATAAAGGCCGGGGGCACatt                                                                                   <  1:2944068/93‑1 (MQ=255)
        ttCATCAACGGGCAGATGACAGATGAAGTAAAAGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGACGGATAAAGGCCGGGGGCACatt                                                                                   >  1:4741910/1‑93 (MQ=255)
         tcatcaACGGGCAGATGACAGATGAAGTAAAAGCCGCGACCAACACGCAGATTTTCAGTATCAACCAGACGGATAAAGGCCGGGGGCACatta                                                                                  <  1:4464372/93‑1 (MQ=255)
         tcatcaACGGGCAGATGACAGATGAAGTAAAAGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGACGGATAAAGGCCGGGGGCACatta                                                                                  >  2:5091200/1‑93 (MQ=255)
         tcatcaACGGGCAGATGACAGATGAAGTAAAAGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGACGGATAAAGGCCGGGGGCACatta                                                                                  <  1:1147754/93‑1 (MQ=255)
          catcaACGGGCAGATGACAGATGAAGTAAAAGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGACGGATAAAGGCCGGGGGCACattat                                                                                 <  1:5531450/93‑1 (MQ=255)
           atcaACGGGCAGATGACAGATGAAGTAAAAGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGACGGATAAAGGCCGGGGGCACattatt                                                                                >  2:6438275/1‑93 (MQ=255)
            tcaACGGGCAGATGACAGATGAAGTAAAAGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGACGGATAAAGGCCGGGGGCACattatta                                                                               >  2:2680265/1‑93 (MQ=255)
             caACGGGCAGATGACAGATGAAGTAAAAGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGACGGATAAAGGCCGGGGGCACattattat                                                                              >  1:2312726/1‑93 (MQ=255)
             caACGGGCAGATGACAGATGAAGTAAAAGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGACGGATAAAGGCCAGGGGCACattattat                                                                              <  1:3063949/93‑1 (MQ=255)
             aaaCCGGGAGGTGACAGGTGAAAGAAAAGCCGCGACCAACCCGCAGATTTTCAGCATCAACCAGACGGATAAAGGCCGGGGGCACattattat                                                                              <  2:5439740/92‑1 (MQ=255)
              agcGGGCAGATGACAGATGAAGTAAAAGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGACGGATAAAGGCCGGGGGCACattattatt                                                                             <  2:1560130/91‑1 (MQ=255)
              aaCGGGCAGATGACAGATGAAGTAAAAGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGACGGATAAAGGCCGGGGGCACattattatt                                                                             <  2:553465/93‑1 (MQ=255)
              aaCGGGCAGATGACAGATGAAGTAAAAGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGACGGATAAAGGCCGGGGGCACattattatt                                                                             >  1:299341/1‑93 (MQ=255)
               aCGGGCAGATGACAGATGAAGTAAAAGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGACGGATAAAGGCCGGGGGCACATTATTATTg                                                                            >  1:4297532/1‑93 (MQ=255)
               aCGGGCAGATGACAGATGAAGTAAAAGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGACGGATAAAGGCCGGGGGCACATTATTATTg                                                                            >  2:1013492/1‑93 (MQ=255)
                  ggCAGATGACAGATGAAGTAAAAGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGACGGATAAAGGCCGGGGGCACATTATTATTGAtt                                                                         >  2:373867/1‑93 (MQ=255)
                    cagatgacagatgaAGTAAAAGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGACGGATAAAGGCCGGGGGCACATTATTATTGATTAc                                                                       >  2:1525526/1‑93 (MQ=255)
                    cagatgacagatgaAGTAAAAGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGACGGATAAAGGCCGGGGGCACATTATTATTGATTAc                                                                       <  2:1179224/93‑1 (MQ=255)
                    cagatgacagatgaAGTAAAAGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGACGGATAAAGGCCGGGGGCACATTATTATTGATTAc                                                                       >  1:1598053/1‑93 (MQ=255)
                      gatgacagatgaAGTAAAAGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGACGGATAAAGGCCGGGGGCACATTATTATTGATTAccc                                                                     >  2:2056532/1‑93 (MQ=255)
                      gatgacagatgaAGTAAAAGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGACGGATAAAGGCCGGGGGCACATTATTATTGATTAccc                                                                     <  1:2454351/93‑1 (MQ=255)
                       atgacacaggaAGGAAAAGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGACGGATAAAGGCCGGGGGCACATTATTATTGATTACCCa                                                                    <  1:5093562/93‑1 (MQ=255)
                        tgacagatgaAGTAAAAGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGACGGATAAAGGCCGGGGGCACATTATTATTGATTACCCAc                                                                   >  2:2374566/1‑93 (MQ=255)
                         gacagatgaAGTAAAAGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGACGGATAAAGGCCGGGGGCACATTATTATTGATTACCCACg                                                                  >  2:3570095/1‑93 (MQ=255)
                           cagatgaAGTAAAAGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGACGGATAAAGGCCGGGGGCACATTATTATTGATTACCCACGcc                                                                <  1:3093898/93‑1 (MQ=255)
                             gatgaAGTAAAAGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGACGGATAAAGGCCAGGGGCACATTATTATTGATTACCCACGCCTg                                                              <  2:4669500/93‑1 (MQ=255)
                               tgaAGTAAAAGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGACGGATAAAGGCCGGGGGCACATTATTATTGATTACCCACGCCTGtt                                                            <  1:66719/93‑1 (MQ=255)
                                 aaGTAAAAGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGACGGATAAAGGCCGGGGGCACATTATTATTGATTACCCACGCCTGTTGa                                                          <  1:2616542/93‑1 (MQ=255)
                                  aGTAAAAGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGACGGATAAAGGCCGGGGGCACATTATTATTGATTACCCACGCCTGTTGaa                                                         <  1:2734681/93‑1 (MQ=255)
                                  aGTAAAAGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGACGGATAAAGGCCGGGGGCACATTATTATTGATTACCCACGCCTGTTGaa                                                         <  2:2987701/93‑1 (MQ=255)
                                   gTAAAAGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGACGGATAAAGGCCGGGGGCACATTATTATTGATTACCCACGCCTGTTGAAt                                                        <  1:6445094/93‑1 (MQ=255)
                                   atAAAAGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGACGGATAAAGGCCGGGGGCACATTATTATTGATTACCCACGCCTGTTGAAt                                                        <  1:1162066/92‑1 (MQ=255)
                                     aaaaGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGACGGATAAAGGCCAGGGGCACATTATTATTGATTACCCACGCCTGTTGAATCa                                                      <  1:1243159/93‑1 (MQ=255)
                                          ccGCGACCAACACGCAGATTTTCAGCATCAACCAGACGGATAAAGGCCGGGGGCACATTATTATTGATTACCCACGCCTGTTGAATCACGGGc                                                 <  2:5596650/93‑1 (MQ=255)
                                           cgcgACCAACACGCAGATTTTCAGCATCAACCAGACGGATAAAGGCCGGGGGCACATTATTATTGATTACCCACGCCTGTTGAATCACGGGCt                                                <  2:2567348/93‑1 (MQ=255)
                                           cgcgACCAACACGCAGATTTTCAGCATCAACCAGACGGATAAAGGCCGGGGGCACATTATTATTGATTACCCACGCCTGTTGAATCACGGGCt                                                <  1:498330/93‑1 (MQ=255)
                                             cgACCAACACGCAGATTTTCAGCATCAACCAGACGGATAAAGGCCAGGGGCACATTATTATTGATTACCCACGCCTGTTGAATCACGGGCTgg                                              <  1:754420/93‑1 (MQ=255)
                                              gACCAACACGCAGATTTTCAGCATCAACCAGACGGATAAAGGCCGGGGGCACATTATTATTGATTACCCACGCCTGTTGAATCACGGGCTggg                                             >  1:1650922/1‑93 (MQ=255)
                                               aCCAACACGCAGATTTTCAGCATCAACCAGACGGATAAAGGCCGGGGGCACATTATTATTGATTACCCACGCCTGTTGAATCACGGGCTGGGt                                            <  2:1306812/93‑1 (MQ=255)
                                                  aaCACGCAGATTTTCAGCATCAACCAGACGGATAAAGGCCGGGGGCACATTATTATTGATTACCCACGCCTGTTGAATCACGGGCTGGGTGAg                                         >  1:1275518/1‑93 (MQ=255)
                                                  aaCACGCAGATTTTCAGCATCAACCAGACGGATAAAGGCCGGGGGCACATTATTATTGATTACCCACGCCTGTTGAATCACGGGCTGGGTGAg                                         <  2:3666063/93‑1 (MQ=255)
                                                   acacGCAGATTTTCAGCATCAACCAGACGGATAAAGGCCGGGGGCACATTATTATTGATTACCCACGCCTGTTGAATCACGGGCTGGGTGAGc                                        >  2:574004/1‑93 (MQ=255)
                                                   acacGCAGATTTTCAGCATCAACCAGACGGATAAAGGCCGGGGGCACATTATTATTGATTACCCACGCCTGTTGAATCACGGGCTGGGTGAGc                                        >  1:1976370/1‑93 (MQ=255)
                                                    cacGCAGATTTTCAGCATCAACCAGACGGATAAAGGCCGGGGGCACATTATTATTGATTACCCACGCCTGTTGAATCACGGGCTGGGTGAGCt                                       <  1:2650306/93‑1 (MQ=255)
                                                     acGCAGATTTTCAGCATCAACCAGACGGATAAAGGCCGGGGGCACATTATTATTGATTACCCACGCCTGTTGAATCACGGGCTGGGTGAGCtg                                      <  1:957885/93‑1 (MQ=255)
                                                      cGCAGATTTTCAGCATCAACCAGACGGATAAAGGCCGGGGGCACATTATTATTGATTACCCACGCCTGTTGAATCACGGGCTGGGTGAGCtgg                                     >  2:5828876/1‑93 (MQ=255)
                                                      cGCAGATTTTCAGCATCAACCAGACGGATAAAGGCCGGGGGCACATTATTATTGATTACCCACGCCTGTTGAATCACGGGCTGGGTGAGCtgg                                     <  1:1919049/93‑1 (MQ=255)
                                                       gCAGATTTTCAGCATCAACCAGACGGATAAAGGCCGGGGGCACATTATTATTGATTACCCACGCCTGTTGAATCACGGGCTGGGTGAGCtggt                                    <  1:4052392/93‑1 (MQ=255)
                                                       gCAGATTTTCAGCATCAACCAGACGGATAAAGGCCGGGGGCACATTATTATTGATTACCCACGCCTGTTGAATCACGGGCTGGGTGAGCtggt                                    <  2:5635875/93‑1 (MQ=255)
                                                         aGATTTTCAGCATCAACCAGACGGATAAAGGCCGGGGGCACATTATTATTGATTACCCACGCCTGTTGAATCACGGGCTGGGTGAGCtggtgg                                  <  1:254399/93‑1 (MQ=255)
                                                         aGATTTTCAGCATCAACCAGACGGATAAAGGCCGGGGGCACATTATTATTGATTACCCACGCCTGTTGAATCACGGGCTGGGTGAGCtggtgg                                  <  1:462963/93‑1 (MQ=255)
                                                         aGATTTTCAGCATCAACCAGACGGATAAAGGCCGGGGGCACATTATTATTGATTACCCACGCCTGTTGAATCACGGGCTGGGTGAGCtggtgg                                  >  1:3768194/1‑93 (MQ=255)
                                                           aTTTTCAGCATCAACCAGACGGATAAAGGCCGGGGGCACATTATTATTGATTACCCACGCCTGTTGAATCACGGGCTGGGTGAGCTGGTGGca                                >  1:2698294/1‑93 (MQ=255)
                                                             tttCAGCATCAACCAGACGGATAAAGGCCGGGGGCACATTATTATTGATTACCCACGCCTGTTGAATCACGGGCTGGGTGAGCTGGTGGcaca                              >  2:3958881/1‑93 (MQ=255)
                                                               tCAGCATCAACCAGACGGATAAAGGCCGGGGGCACATTATTATTGATTACCCACGCCTGTTGAATCACGGGCTGGGTGAGCTGGTGGCACAGa                            <  1:5890199/93‑1 (MQ=255)
                                                                cAGCATCAACCAGACGGATAAAGGCCTGGGGCACATTATTATTGATTACCCACGCCTGTTGAATCACGGGCTGGGTGAGCTGGTGGCACAGAt                           <  2:3509078/93‑1 (MQ=255)
                                                                cAGCATCAACCAGACGGATAAAGGCCGGGGGCACATTATTATTGATTACCCACGCCTGTTGAATCACGGGCTGGGTGAGCTGGTGGCACAGAt                           <  1:4530097/93‑1 (MQ=255)
                                                                   cATCAACCAGACGGATAAAGGCCGGGGGCACATTATTATTGATTACCCACGCCTGTTGAATCACGGGCTGGGTGAGCTGGTGGCACAGATgca                        <  1:5610175/93‑1 (MQ=255)
                                                                   cATCAACCAGACGGATAAAGGCAGGGGGCACATTATTATTGATTACCCACGCCTGTTGAATCACGGGCTGGGTGAGCTGGTGGCACAGATgca                        <  2:3334821/93‑1 (MQ=255)
                                                                    aTCAACCAGACGGATAAAGGCCGGGGGCACATTATTATTGATTAACCACGCCTGTTGAATCACGGGCTGGGTGAGCTGGAGGAACAGATgcag                       >  1:836391/1‑93 (MQ=255)
                                                                     tCAACCAGACGGATAAAGGCCGGGGGCACATTATTATTGATTACCCACGCCTGTTGAATCACGGGCTGGGTGAGCTGGTGGCACAGATgcagc                      <  2:4111156/93‑1 (MQ=255)
                                                                     tCAACCAGACGGATAAAGGCCGGGGGCACATTATTATTGATTACCCACGCCTGTTGAATCACGGGCTGGGTGAGCTGGTGGCACAGATgcagc                      >  2:1483705/1‑93 (MQ=255)
                                                                     tCAACCAGACGGATAAAGGCCGGGGGCACATTATTATTGATTACCCACGCCTGTTGAATCACGGGCTGGGTGAGCTGGTGGCACAGATgcagc                      >  1:3955334/1‑93 (MQ=255)
                                                                     acAACCAGACGGATAAAGGCCGGGGGCACATTATTATTGATTACCCACGCCTGTTGAATCACGGGCTGGGTGAGCTGGTGGCACAGATgcagc                      <  1:5718469/92‑1 (MQ=255)
                                                                       aaCCAGACGGATAAAGGCCGGGGGCACATTATTATTGATTACCCACGCCTGTTGAATCACGGGCTGGGTGAGCTGGTGGCACAGATGCAGCaa                    <  1:3114466/93‑1 (MQ=255)
                                                                       aaCCAGACGGATAAAGGCCGGGGGCACATTATTATTGATTACCCACGCCTGTTGAATCACGGGCTGGGTGAGCTGGTGGCACAGATGCAGCaa                    <  2:4401112/93‑1 (MQ=255)
                                                                         ccAGACGGATAAAGGCCGGGGGCACATTATTATTGATTACCCACGCCTGTTGAATCACGGGCTGGGTGAGCTGGTGGCACAGATGCAGCAACa                  <  1:1067540/93‑1 (MQ=255)
                                                                         ccAGACGGATAAAGGCCGGGGGCACATTATTATTGATTACCCACGCCTGTTGAATCACGGGCTGGGTGAGCTGGTGGCACAGATGCAGCAACa                  <  2:4925077/93‑1 (MQ=255)
                                                                          cAGACGGATAAAGGCCGGGGGCACATTATTATTGATTACCCACGCCTGTTGAATCACGGGCTGGGTGAGCTGGTGGCACAGATGCAGCAACAt                 >  2:324000/1‑93 (MQ=255)
                                                                            gACGGGTAAAGGCCGGGGGCACATTATTATTGATTACCCACGCCTGTTGAATGACGGGCTGGGTGAGCTGGCGGCACCGGTGCAGCAACATTg               >  2:1678701/1‑93 (MQ=255)
                                                                            gACGGATAAAGGCCGGGGGCACATTATTATTGATTACCCACGCCTGTTGAATCACGGGCTGGGTGAGCTGGTGGCACAGATGCAGCAACATTg               <  1:1521598/93‑1 (MQ=255)
                                                                            gACGGATAAAGGCCGGGGGCACATTATTATTGATTACCCACGCCTGTTGAATCACGGGCTGGGTGAGCTGGTGGCACAGATGCAGCAACATTg               <  2:4598603/93‑1 (MQ=255)
                                                                             aCGGATAAAGGCCGGGGGCACATTATTATTGATTACCCACGCCTGTTGAATCACGGGCTGGGTGAGCTGGTGGCACAGATGCAGCAACATTGt              <  1:5813649/93‑1 (MQ=255)
                                                                             aCGGATAAAGGCCGGGGGCACATTATTATTGATTACCCACGCCTGTTGAATCACGGGCTGGGTGAGCTGGTGGCACAGATGCAGCAACATTGt              <  1:6085157/93‑1 (MQ=255)
                                                                              cGGATAAAGGCCGGGGGCACATTATTATTGATTACCCACGCCTGTTGAATCACGGGCTGGGTGAGCTGGTGGCACAGATGCAGCAACATTGTc             <  2:5269764/93‑1 (MQ=255)
                                                                               ggATAAAGGCCGGGGGCACATTATTATTGATTACCCACGCCTGTTGAATCACGGGCTGGGTGAGCTGGTGGCACAGATGCAGCAACATTGTCa            >  2:2851259/1‑93 (MQ=255)
                                                                               ggATAAAGGCCGGGGGCACATTATTATTGATTACCCACGCCTGTTGAATCACGGGCTGGGTGAGCTGGTGGCACAGATGCAGCAACATTGTCa            >  1:2903181/1‑93 (MQ=255)
                                                                               ggATAAAGGCCGGGGGCACATTATTATTGATTACCCACGCCTGTTGAATCACGGGCTGGGTGAGCTGGTGGCACAGATGCAGCAACATTGTCa            <  2:1958610/93‑1 (MQ=255)
                                                                                  tAAAGGCCGGGGGCACATTATTATTGATTACCCACGCCTGTTGAATCACGGGCTGGGTGAGCTGGTGGCACAGATGCAGCAACATTGTCAGCa         <  1:5825776/93‑1 (MQ=255)
                                                                                  tAAAGGCCGGGGGCACATTATTATTGATTACCCACGCCTGTTGAATCACGGGCTGGGTGAGCTGGTGGCACAGATGCAGCAACATTGTCAGCa         >  1:5394860/1‑93 (MQ=255)
                                                                                   aaaGGCCGGGGGCACATTATTATTGATTACCCACGCCTGTTGAATCACGGGCTGGGTGAGCTGGTGGCACAGATGCAGCAACATTGTCAGCaa        >  2:4394064/1‑93 (MQ=255)
                                                                                   aaaGGCCAGGGGCACATTATTATTGATTACCCACGCCTGTTGAATCACGGGCTGGGTGAGCTGGTGGCACAGATGCAGCAACATTGTCAGCaa        <  2:4842452/93‑1 (MQ=255)
                                                                                     aGGCCGGGGGCACATTATTATTGATTACCCACGCCTGTTGAATCACGGGCTGGGTGAGCTGGTGGCACAGATGCAGCAACATTGTCAGCAACa      <  2:2245331/93‑1 (MQ=255)
                                                                                     aGGCCGGGGGCACATTATTATTGATTACCCACGCCTGTTGAATCACGGGCTGGGTGAGCTGGTGGCACAGATGCAGCAACATTGTCAGCAACa      <  2:2610942/93‑1 (MQ=255)
                                                                                       gCCGGGGGCACATTATTATTGATTACCCACGCCTGTTGAATCACGGGCTGGGTGAGCTGGTGGCACAGATGCAGCAACATTGTCAGCAACAGc    >  1:845906/1‑93 (MQ=255)
                                                                                       gCCGGGGGCACATTATTATTGATTACCCACGCCTGTTGAATCACGGGCTGGGTGAGCTGGTGGCACAGATGCAGCAACATTGTCAGCAACAGc    <  2:4201654/93‑1 (MQ=255)
                                                                                         cAGGGGCACATTATTATTGATTACCCACGCCTGTTGAATCACGGGCTGGGTGAGCTGGTGGCACAGATGCAGCAACATTGTCAGCAACAGCCg  >  2:6024421/1‑93 (MQ=255)
                                                                                          |                                                                                           
TGAAAGATTTCATCAACGGGCAGATGACAGATGAAGTAAAAGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGACGGATAAAGGCCAGGGGCACATTATTATTGATTACCCACGCCTGTTGAATCACGGGCTGGGTGAGCTGGTGGCACAGATGCAGCAACATTGTCAGCAACAGCCG  >  NC_000913/4141944‑4142125

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CGATGAAAGATTTCATCAACGGGCAGATGACAGATGAAGTAAAAGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGACGGATAAAGGCCAGGGGCACATTATTATTGATTACCCACGCCTGTTGAATCACGGGCTGGGTGAGCTGGTGGCACAGATGCAGCAACATTGTCAGCAACAGCCGG  >  NC_000913/4141941‑4142126
                                                                                             |                                                                                            
CGATGAAAGATTTCATCAACGGGCAGATGACAGATGAAGTAAAAGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGACGGATAAAGGCCG                                                                                              <  SRR4302537.1836276/94‑1 (MQ=60)
CGATGAAAGATTTCATCAACGGGCAGATGACAGATGAAGTAAAAGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGACGGATAAAGGCCG                                                                                              <  SRR4302537.1624191/94‑1 (MQ=60)
CGATGAAAGATTTCATCAACGGGCAGATGACAGATGAAGTAAAAGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGACGGATAAAGGCCG                                                                                              >  SRR4302537.5918460/1‑94 (MQ=60)
 GATGAAAGATTTCATCAACGGGCAGATGACAGATGAAGTAAAAGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGACGGATAAAGGCCGG                                                                                             <  SRR4302537.807969/94‑1 (MQ=60)
 GATGAAAGATTTCATCAACGGGCAGATGACAGATGAAGTAAAAGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGACGGATAAAGGCCGG                                                                                             <  SRR4302537.2100552/94‑1 (MQ=60)
 GATGAAAGATTTCATCAACGGGCAGATGACAGATGAAGTAAAAGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGACGGATAAAGGCCGG                                                                                             <  SRR4302537.2770486/94‑1 (MQ=60)
  ATGACAGATTTCATCAACGGGCAGATGACAGATGAAGTAAAAGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGACGGATAAAGGCCGGG                                                                                            >  SRR4302537.5747309/1‑94 (MQ=60)
   TGAAAGATTTCATCAACGGGCAGATGACAGATGAAGTAAAAGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGACGGATAAAGGCCGGGG                                                                                           <  SRR4302537.311592/94‑1 (MQ=60)
   TGAAAGATTTCATCAACGGGCAGATGACAGATGAAGTAAAAGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGACGGATAAAGGCCGGGG                                                                                           >  SRR4302537.5784206/1‑94 (MQ=60)
   TGAAAGATTTCATCAACGGGCAGATGACAGATGAAGTAAAAGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGACGGATAAAGGCCGGGG                                                                                           >  SRR4302537.2851893/1‑94 (MQ=60)
   TGAAAGATTTCATCAACGGGCAGATGACAGATGAAGTAAAAGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGACGGATAAAGGCCGGGG                                                                                           >  SRR4302537.1636722/1‑94 (MQ=60)
    GAAAGATTTCATCAACGGGCAGATGACAGATGAAGTAAAAGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGACGGATAAAGGCCGGGGG                                                                                          <  SRR4302537.6455059/94‑1 (MQ=60)
    GAAAGATTTCATCAACGGGCAGATGACAGATGAAGTAAAAGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGACGGATAAAGGCCGGGGG                                                                                          >  SRR4302537.5617471/1‑94 (MQ=60)
    GAAAGATTTCATCAACGGGCAGATGACAGATGAAGTAAAAGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGACGGATAAAGGCCGGGGG                                                                                          >  SRR4302537.561335/1‑94 (MQ=60)
     AAAGATTTCATCAACGGGCAGATGACAGATGAAGTAAAAGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGACGGATAAAGGCCGGGGGC                                                                                         >  SRR4302537.3086352/1‑94 (MQ=60)
     AAAGATTTCATCAACGGGCAGATGACAGATGAAGTAAAAGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGACGGATAAAGGCCGGGGGC                                                                                         <  SRR4302537.6462812/94‑1 (MQ=60)
     AAAGATTTCATCAACGGGCAGATGACAGATGAAGTAAAAGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGACGGATAAAGGCCGGGGGC                                                                                         <  SRR4302537.3957067/94‑1 (MQ=60)
       AGATTTCATCAACGGGCAGATGACAGATGAAGTAAAAGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGACGGATAAAGGCCGGGGGCAC                                                                                       <  SRR4302537.6388385/94‑1 (MQ=60)
       AGATTTCATCAACGGGCAGATGACAGATGAAGTAAAAGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGACGGATAAAGGCCGGGGGCAC                                                                                       >  SRR4302537.1443300/1‑94 (MQ=60)
       AGATTTCATCAACGGGCAGATGACAGATGAAGTAAAAGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGACGGATAAAGGCCAGGGGCAC                                                                                       <  SRR4302537.3732852/94‑1 (MQ=60)
        GATTTCATCAACGGGCAGATGACAGATGAAGTAAAAGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGACGGATAAAGGCCGGGGGCACA                                                                                      >  SRR4302537.5290724/1‑94 (MQ=60)
         ATTTCATCAACGGGCAGATGACAGATGAAGTAAAAGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGACGGATAAAGGCCGGGGGCACAT                                                                                     <  SRR4302537.3547621/94‑1 (MQ=60)
         ATTTCATCAACGGGCAGATGACAGATGAAGTAAAAGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGACGGATAAAGGCCGGGGGCACAT                                                                                     <  SRR4302537.3891905/94‑1 (MQ=60)
          TTTCATCAACGGGCAGATGACAGATGAAGTAAAAGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGACGGATAAAGGCCGGGGGCACATT                                                                                    >  SRR4302537.4774682/1‑94 (MQ=60)
          TTTCATCAACGGGCAGATGACAGATGAAGTAAAAGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGACGGATAAAGGCCGGGGGCACATT                                                                                    <  SRR4302537.2055762/94‑1 (MQ=60)
           TTCATCAACGGGCAGATGACAGATGAAGTAAAAGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGACGGATAAAGGCCGGGGGCACATTA                                                                                   >  SRR4302537.2554799/1‑94 (MQ=60)
           TTCATCAACGGGCAGATGACAGATGAAGTAAAAGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGACGGATAAAGGCCGGGGGCACATTA                                                                                   <  SRR4302537.2964733/94‑1 (MQ=60)
           TTCATCAACGGGCAGATGACAGATGAAGTAAAAGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGACGGATAAAGGCCGGGGGCACATTA                                                                                   <  SRR4302537.1271279/94‑1 (MQ=60)
            TCATCAACGGGCAGATGACAGATGAAGTAAAAGCCGCGACCAACACGCAGATTTTCAGTATCAACCAGACGGATAAAGGCCGGGGGCACATTAT                                                                                  <  SRR4302537.4495248/94‑1 (MQ=60)
            TCATCAACGGGCAGATGACAGATGAAGTAAAAGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGACGGATAAAGGCCGGGGGCACATTAT                                                                                  <  SRR4302537.1155734/94‑1 (MQ=60)
             CATCAACGGTCAGATGACAGATGAAGTAAAAGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGACGGATAAAGGCCGGGGGCACATTATT                                                                                 <  SRR4302537.1413630/94‑1 (MQ=60)
             CATCAACGGGCAGATGACAGATGAAGTAAAAGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGACGGATAAAGGCCGGGGGCACATTATT                                                                                 <  SRR4302537.5569211/94‑1 (MQ=60)
              CTCAACGGGCAGATGACAGATGAAGTAAAAGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGACGGATAAAGGCCGGGGGCACATTATTA                                                                                <  SRR4302537.4741339/94‑1 (MQ=60)
               TCAACGGGCAGATGACAGATGAAGTAAAAGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGACGGATAAAGGCCGGGGGCACATTATTAT                                                                               >  SRR4302537.2329051/1‑94 (MQ=60)
               TCAACGGGCAGATGACAGATGAAGTAAAAGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGACGGATAAAGGCCAGGGGCACATTATTAT                                                                               <  SRR4302537.1862990/94‑1 (MQ=60)
                CAACGGGCAGATGACAGATGAAGTAAAAGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGACGGATAAAGGCCGGGGGCACATTATTATT                                                                              >  SRR4302537.301429/1‑94 (MQ=60)
                CAACGGGCAGATGACAGATGAAGTAAAAGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGACGGATAAAGGCCGGGGGCACATTATTATT                                                                              >  SRR4302537.2999590/1‑94 (MQ=60)
                CAACGGGCAGATGACAGATGAAGTAAAAGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGACGGATAAAGGCCAGGGGCACATTATTATT                                                                              <  SRR4302537.3085294/94‑1 (MQ=60)
                 AACGGGCAGATGACAGATGAAGTAAAAGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGACGGATAAAGGCCGGGGGCACATTATTATTG                                                                             >  SRR4302537.4327403/1‑94 (MQ=60)
                 AACGGGCAGATGACAGATGAAGTAAAAGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGACGGATAAAGGCCGGGGGCACATTATTATTG                                                                             <  SRR4302537.384713/94‑1 (MQ=60)
                    GGGCAGATGACAGATGAAGTAAAAGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGACGGATAAAGGCCGGGGGCACATTATTATTGATT                                                                          >  SRR4302537.5023781/1‑94 (MQ=60)
                      GCAGATGACAGATGAAGTAAAAGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGACGGATAAAGGCCGGGGGCACATTATTATTGATTAC                                                                        >  SRR4302537.1609218/1‑94 (MQ=60)
                        AGATGACAGATGAAGTAAAAGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGACGGATAAAGGCCGGGGGCACATTATTATTGATTACCC                                                                      <  SRR4302537.683788/94‑1 (MQ=60)
                        AGATGACAGATGAAGTAAAAGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGACGGATAAAGGCCGGGGGCACATTATTATTGATTACCC                                                                      >  SRR4302537.1145008/1‑94 (MQ=60)
                        AGATGACAGATGAAGTAAAAGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGACGGATAAAGGCCGGGGGCACATTATTATTGATTACCA                                                                      >  SRR4302537.943047/1‑94 (MQ=60)
                        AGATGACAGATGAAGTAAAAGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGACGGATAAAGGCCGGAGGCACATTATTATTGATTACCC                                                                      <  SRR4302537.407529/94‑1 (MQ=60)
                         GATGACAGATGAAGTAAAAGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGACGGATAAAGGCCGGGGGCACATTATTATTGATTACCCA                                                                     <  SRR4302537.2471614/94‑1 (MQ=60)
                          ATGACACAGGAAGGAAAAGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGACGGATAAAGGCCGGGGGCACATTATTATTGATTACCCAC                                                                    <  SRR4302537.5128631/94‑1 (MQ=60)
                           TGACAGATGAAGTAAAAGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGACGGATAAAGGCCGGGGGCACATTATTATTGATTACCCACG                                                                   <  SRR4302537.3739400/94‑1 (MQ=60)
                              CAGATGAAGTAAAAGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGACGGATAAAGGCCGGGGGCACATTATTATTGATTACCCACGCCT                                                                <  SRR4302537.3115480/94‑1 (MQ=60)
                                GATGAAGTAAAAGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGACGGATAAAGGCCGGGGGCACATTATTATTGATTACCCACGCCTGT                                                              <  SRR4302537.3349277/94‑1 (MQ=60)
                                 ATGAAGTAAAAGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGACGGATAAAGGCCGGGGGCACATTATTATTGATTACCCACGCCTGTT                                                             >  SRR4302537.564351/1‑94 (MQ=60)
                                  TGAAGTAAAAGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGACGGATAAAGGCCGGGGGCACATTATTATTGATTACCCACGCCTGTTG                                                            <  SRR4302537.67212/94‑1 (MQ=60)
                                    AAGTAAAAGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGACGGATAAAGGCCGGGGGCACATTATTATTGATTACCCACGCCTGTTGAA                                                          <  SRR4302537.2634841/94‑1 (MQ=60)
                                     AGTAAAAGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGACGGATAAAGGCCGGGGGCACATTATTATTGATTACCCACGCCTGTTGAAT                                                         <  SRR4302537.2753764/94‑1 (MQ=60)
                                      GTAAAAGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGACGGATAAAGGCCGGGGGCACATTATTATTGTTTACCCACGCATGTTGAATC                                                        >  SRR4302537.575985/1‑94 (MQ=60)
                                      GTAAAAGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGACGGATAAAGGCCGGGGGCACATTATTATTGATTACCCACGCCTGTTGAATC                                                        <  SRR4302537.2701644/94‑1 (MQ=60)
                                      GTAAAAGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGACGGATAAAGGCCGGGGGCACATTATTATTGATTACCCACGCCTGTTGAATC                                                        <  SRR4302537.6488817/94‑1 (MQ=60)
                                      ATAAAAGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGACGGATAAAGGCCGGGGGCACATTATTATTGATTACCCACGCCTGTTGAATC                                                        <  SRR4302537.1170149/94‑1 (MQ=60)
                                        AAAAGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGACGGATAAAGGCCAGGGGCACATTATTATTGATTACCCACGCCTGTTGAATCAC                                                      <  SRR4302537.1251820/94‑1 (MQ=60)
                                         AAAGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGACGGATAAAGGCCGGGGGCACATTATTATTGATTACCCACGCCTGTTGAATCACG                                                     <  SRR4302537.4571574/94‑1 (MQ=60)
                                         AAAGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGACGGATAAAGGCCGGGGGCACATTATTATTGATTACCCACGCCTGTTGAATCACG                                                     <  SRR4302537.4515183/94‑1 (MQ=60)
                                           AGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGACGGATAAAGGCCGGGGGCACATTATTATTGATTACCCACGCCTGTTGAATCACGGG                                                   <  SRR4302537.2631295/94‑1 (MQ=60)
                                           AGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGACGGATAAAGGCCGGGGGCACATTATTATTGATTACCCACGCCTGTTGAATCACGGG                                                   <  SRR4302537.3065357/94‑1 (MQ=60)
                                             CCGCGACCAACACGCAGATTTTCAGCATCAACCAGACGGATAAAGGCCGGGGGCACATTATTATTGATTACCCACGCCTGTTGAATCACGGGCT                                                 <  SRR4302537.5891733/94‑1 (MQ=60)
                                             CCGCGACCAACACGCAGATTTTCAGCATCAACCAGACGGATAAAGGCCGGGGGCACATTATTATTGATTACCCACGCCTGTTGAATCACGGGCT                                                 >  SRR4302537.4177333/1‑94 (MQ=60)
                                              CGCGACCAACACGCAGATTTTCAGCATCAACCAGACGGATAAAGGCCGGGGGCACATTATTATTGATTACCCACGCCTGTTGAATCACGGGCTG                                                <  SRR4302537.501756/94‑1 (MQ=60)
                                                CGACCAACACGCAGATTTTCAGCATCAACCAGACGGATAAAGGCCGGGGGCACATTATTATTGATTACCCACGCCTGTTGAATCACGGGCTGGG                                              >  SRR4302537.1662502/1‑94 (MQ=60)
                                                CGACCAACACGCAGATTTTCAGCATCAACCAGACGGATAAAGGCCAGGGGCACATTATTATTGATTACCCACGCCTGTTGAATCACGGGCTGGG                                              <  SRR4302537.759564/94‑1 (MQ=60)
                                                  ACCAACACGCAGATTTTCAGCATCAACCAGACGGATAAAGGCCGGGGGCACATTATTATTGATTACCCACGCCTGTTGAATCACGGGCTGGGTG                                            >  SRR4302537.4857060/1‑94 (MQ=60)
                                                   CCAACACGCAGATTTTCAGCATCAACCAGACGGATACAGGACGGGGGCACATTATTATTGATTACCGACGCCTGTTtaatacggtgctggatga                                           >  SRR4302537.1751996/1‑76 (MQ=60)
                                                    CAACACGCAGATTTTCAGCATCAACCAGACGGATAAAGGCCGGGGGCACATTATTATTGATTACCCACGCCTGTTGAATCACGGGCTGGGTGAG                                          >  SRR4302537.972119/1‑94 (MQ=60)
                                                    CAACACGCAGATTTTCAGCATCAACCAGACGGATAAAGGCCGGGGGCACATTATTATTGATTACCCACGCCTGTTGAATCACGGGCTGGGTGAG                                          >  SRR4302537.2725834/1‑94 (MQ=60)
                                                    CAACACGCAGATTTTCAGCATCAACCAGACGGATAAAGGCCGGGGGCACATTATTATTGATTACCCACGCCTGTTGAATCACGGGCTGGGTGAG                                          >  SRR4302537.5855744/1‑94 (MQ=60)
                                                    CAACACGCAGATTTTCAGCATCAACCAGACGGATAAAGGCCGGGGGCACATTATTATTGATTACCCACGCCTGTTGAATCACGGGCTGGGTGAG                                          >  SRR4302537.1998569/1‑94 (MQ=60)
                                                    CAACACGCAGATTTTCAGCATCAACCAGACGGATAAAGGCCGGGGGCACATTATTATTGATTACCCACGCCTGTTGAATCACGGGCTGGGTGAG                                          >  SRR4302537.1284389/1‑94 (MQ=60)
                                                     AACACGCAGATTTTCAGCATCAACCAGACGGATAAAGGCCGGGGGCACATTATTATTGATTACCCACGCCTGTTGAATCACGGGCTGGGTGAGC                                         <  SRR4302537.150757/94‑1 (MQ=60)
                                                     AACACGCAGATTTTCAGCATCAACCAGACGGATAAAGGCCGGGGGCACATTATTATTGATTACCCACGCCTGTTGAATCACGGGCTGGGTGAGC                                         >  SRR4302537.1990104/1‑94 (MQ=60)
                                                      ACACGCAGATTTTCAGCATCAACCAGACGGATAAAGGCCGGGGGCACATTATTATTGATTACCCACGCCTGTTGAATCACGGGCTGGGTGAGCT                                        >  SRR4302537.829292/1‑94 (MQ=60)
                                                       CACGCAGATTTTCAGCATCAACCAGACGGATAAAGGCCGGGGGCACATTATTATTGATTACCCACGCCTGTTGAATCACGGGCTGGGTGAGCTG                                       <  SRR4302537.2668845/94‑1 (MQ=60)
                                                        ACGCAGATTTTCAGCATCAACCAGACGGATAAAGGCCGGGGGCACATTATTATTGATTACCCACGCCTGTTGAATCACGGGCTGGGTGAGCTGG                                      <  SRR4302537.964493/94‑1 (MQ=60)
                                                         CGCAGATTTTCAGCATCAACCAGACGGATAAAGGCCGGGGGCACATTATTATTGATTACCCACGCCTGTTGAATCACGGGCTGGGTGAGCTGGT                                     <  SRR4302537.1932373/94‑1 (MQ=60)
                                                         CGCAGATTTTCAGCATCAACCAGACGGATAAAGGCCGGGGGCACATTATTATTGATTACCCACGCCTGTTGAATCACGGGCTGGGTGAGCTGGT                                     >  SRR4302537.6020161/1‑94 (MQ=60)
                                                         CGCAGATTTTCAGCATCAACCAGACGGATAAAGGCCGGGGGCACATTATTATTGATTACCCACGCCTGTTGAATCACGGGCTGGGTGAGCTGGT                                     >  SRR4302537.5808006/1‑94 (MQ=60)
                                                          GCAGATTTTCAGCATCAACCAGACGGATAAAGGCCGGGGGCACATTATTATTGATTACCCACGCCTGTTGAATCACGGGCTGGGTGAGCTGGTG                                    <  SRR4302537.4080729/94‑1 (MQ=60)
                                                           CAGATTTTCAGCATCAACCAGACGGATAAAGGCCGGGGGCACATTATTATTGATTACCCACGCCTGTTGAATCACGGGCTGGGTGAGCTGGTGG                                   >  SRR4302537.194851/1‑94 (MQ=60)
                                                           CAGATTTTCAGCATCAACCAGACGGATAAAGGCCGGGGGCACATTATTATTGATTACCCACGCCTGTTGAATCACGGGCTGGGTGAGCTGGTGG                                   >  SRR4302537.3794650/1‑94 (MQ=60)
                                                            AGATTTTCAGCATCAACCAGACGGATAAAGGCCGGGGGCACATTATTATTGATTACCCACGCCTGTTGAATCACGGGCTGGGTGAGCTGGTGGC                                  <  SRR4302537.256203/94‑1 (MQ=60)
                                                            AGATTTTCAGCATCAACCAGACGGATAAAGGCCGGGGGCACATTATTATTGATTACCCACGCCTGTTGAATCACGGGCTGGGTGAGCTGGTGGC                                  <  SRR4302537.466150/94‑1 (MQ=60)
                                                             GATTTTCAGCATCAACCAGACGGATAAAGGCCGGGGGCACATTATTATTGATTACCCACGCCTGTTGAATCACGGGCTGGGTGAGCTGGTGGCA                                 >  SRR4302537.2717146/1‑94 (MQ=60)
                                                              ATTTTCAGCATCAACCAGACGGATAAAGGCCGGGGGCACATTATTATTGATTACCCACGCCTGTTGAATCACGGGCTGGGTGAGCTGGTGGCAC                                >  SRR4302537.1599926/1‑94 (MQ=60)
                                                                TTTCAGCATCAACCAGACGGATAAAGGCCGGGGGCACATTATTATTGATTACCCACGCCTGTTGAATCACGGGCTGGGTGAGCTGGTGGCACAG                              <  SRR4302537.1166650/94‑1 (MQ=60)
                                                                  TCAGCATCAACCAGACGGATAAAGGCCGGGGGCACATTATTATTGATTACCCACGCCTGTTGAATCACGGGCTGGGTGAGCTGGTGGCACAGAT                            <  SRR4302537.5930615/94‑1 (MQ=60)
                                                                  TCAGCATCAACCAGACGGATAAAGGCCGGGGGCACATTATTATTGATTACCCACGCCTGTTGAATCACGGGCTGGGTGAGCTGGTGGCACAGAT                            <  SRR4302537.2123396/94‑1 (MQ=60)
                                                                   CAGCATCAACCAGACGGATAAAGGCCGGGGGCACATTATTATTGATTACCCACGCCTGTTGAATCACGGGCTGGGTGAGCTGGTGGCACAGATG                           <  SRR4302537.4561462/94‑1 (MQ=60)
                                                                     GCATCAACCAGACGGATAAAGGCCGGGGGCACATTATTATTGATTACCCACGCCTGTTGAATCACGGGCTGGGTGAGCTGGTGGCACAGATGCA                         <  SRR4302537.6506737/94‑1 (MQ=60)
                                                                      CATCAACCAGACGGATAAAGGCCGGGGGCACATTATTATTGATTACCCACGCCTGTTGAATCACGGGCTGGGTGAGCTGGTGGCACAGATGCAG                        <  SRR4302537.5648531/94‑1 (MQ=60)
                                                                      CATCAACCAGACGGATAAAGGCCGGGGGCACATTATTATTGATTACCCACGCCTGTTGAATCACGGGCTGGGTGAGCTGGTGGCACAGATGCAG                        <  SRR4302537.3614619/94‑1 (MQ=60)
                                                                      CATCAACCAGACGGATAAAGGCCGGGGGCACATTATTATTGATTACCCACGCCTGTTGAATCACGGGCTGGGTGAGCTGGTGGCACAGATGCAG                        >  SRR4302537.2373536/1‑94 (MQ=60)
                                                                      CATCAACCAGACGGATAAAGGCCGGGGGCACATTATTATTGATTAACCACGCCTGTTGAATCACGGGCTGGGTGAGCTGGAGGAACAGATGCAG                        >  SRR4302537.842117/1‑94 (MQ=60)
                                                                       ATCAACCAGACGGATAAAGGCCGGGGGCACATTATTATTGATTACCCACGCCTGTTGAATCACGGGCTGGGTGAGCTGGTGGCACAGATGCAGC                       <  SRR4302537.5519969/94‑1 (MQ=60)
                                                                       ATCAACCAGACGGATAAAGGCCGGGGGCACATTATTATTGATTACCCACGCCTGTTGAATCACGGGCTGGGTGAGCTGGTGGCACAGATGCAGC                       >  SRR4302537.3983005/1‑94 (MQ=60)
                                                                        ACAACCAGACGGATAAAGGCCGGGGGCACATTATTATTGATTACCCACGCCTGTTGAATCACGGGCTGGGTGAGCTGGTGGCACAGATGCAGCA                      <  SRR4302537.5757607/94‑1 (MQ=60)
                                                                          AACCAGACGGATAAAGGCCGGGGGCACATTATTATTGATTACCCACGCCTGTTGAATCACGGGCTGGGTGAGCTGGTGGCACAGATGCAGCAAC                    <  SRR4302537.3136218/94‑1 (MQ=60)
                                                                            CCAGACGGATAAAGGCCGGGGGCACATTATTATTGATTACCCACGCCTGTTGAATCACGGGCTGGGTGAGCTGGTGGCACAGATGCAGCAACAT                  <  SRR4302537.1074893/94‑1 (MQ=60)
                                                                             CAGACGGATAAAGGCCGGGGGCACATTATTATTGATTACCCACGCCTGTTGAATCACGGGCTGGGTGAGCTGGTGGCACAGATGCAGCAACATT                 >  SRR4302537.6497895/1‑94 (MQ=60)
                                                                             CAGACGGATAAAGGCCGGGGGCACATTATTATTGATTACCCACGCCTGTTGAATCACGGGCTGGGTGAGCTGGTGGCACAGATGCAGCAACATT                 >  SRR4302537.2658543/1‑94 (MQ=60)
                                                                               GACGGATAAAGGCCGGGGGCACATTATTATTGATTACCCACGCCTGTTGAATCACGGGCTGGGTGAGCTGGTGGCACAGATGCAGCAACATTGT               <  SRR4302537.2939914/94‑1 (MQ=60)
                                                                               GACGGATAAAGGCCGGGGGCACATTATTATTGATTACCCACGCCTGTTGAATCACGGGCTGGGTGAGCTGGTGGCACAGATGCAGCAACATTGT               <  SRR4302537.1532225/94‑1 (MQ=60)
                                                                                ACGGATAAAGGCCGGGGGCACATTATTATTGATTACCCACGCCTGTTGAATCACGGGCTGGGTGAGCTGGTGGCACAGATGCAGCAACATTGTC              <  SRR4302537.6126961/94‑1 (MQ=60)
                                                                                ACGGATAAAGGCCGGGGGCACATTATTATTGATTACCCACGCCTGTTGAATCACGGGCTGGGTGAGCTGGTGGCACAGATGCAGCAACATTGTC              <  SRR4302537.5853498/94‑1 (MQ=60)
                                                                                 CGGATAAAGGCCGGGGGCACATTATTATTGATTACCCACGCCTGTTGAATCACGGGCTGGGTGAGCTGGTGGCACAGATGCAGCAACATTGTCA             >  SRR4302537.2923577/1‑94 (MQ=60)
                                                                                    ATAAAGGCCGGGGGCACATTATTATTGATTACCCACGCCTGTTGAATCACGGGCTGGGTGAGCTGGTGGCACAGATGCAGCAACATTGTCAGCA          >  SRR4302537.5431700/1‑94 (MQ=60)
                                                                                     TAAAGGCCGGGGGCACATTATTATTGATTACCCACGCCTGTTGAATCACGGGCTGGGTGAGCTGGTGGCACAGATGCAGCAACATTGTCAGCAA         <  SRR4302537.5865704/94‑1 (MQ=60)
                                                                                      AAAGGCCGGGGGCACATTATTATTGATTACCCACGCCTGTTGAATCACGGGCTGGGTGAGCTGGTGGCACAGATGCAGCAACATTGTCAGCAAC        <  SRR4302537.84485/94‑1 (MQ=60)
                                                                                         GGCCGGGGGCACATTATTATTGATTACCCACGCCTGTTGAATCACGGGCTGGGTGAGCTGGTGGCACAGATGCAGCAACATTGTCAGCAACAGC     >  SRR4302537.851702/1‑94 (MQ=60)
                                                                                          GCCGGGGGCACATTATTATTGATTACCCACGCCTGTTGAATCACGGGCTGGGTGAGCTGGTGGCACAGATGCAGCAACATTGTCAGCAACAGCC    <  SRR4302537.1173132/94‑1 (MQ=60)
                                                                                          GCCGGGGGCACATTATTATTGATTACCCACGCCTGTTGAATCACGGGCTGGGTGAGCTGGTGGCACAGATGCAGCAACATTGTCAGCAACAGCC    <  SRR4302537.4483141/94‑1 (MQ=60)
                                                                                           CCGGGGGCACATTATTATTGATTACCCACGCCTGTTGAATCACGGGCTGGGTGAGCTGGTGGCACAGATGCAGCAACATTGTCAGCAACAGCCG   <  SRR4302537.4483903/94‑1 (MQ=60)
                                                                                            CGGGGGCACATTATTATTGATTACCCACGCCTGTTGAATCACGGGCTGGGTGAGCTGGTGGCACAGATGCAGCAACATTGTCAGCAACAGCCGG  <  SRR4302537.6490048/94‑1 (MQ=60)
                                                                                             |                                                                                            
CGATGAAAGATTTCATCAACGGGCAGATGACAGATGAAGTAAAAGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGACGGATAAAGGCCAGGGGCACATTATTATTGATTACCCACGCCTGTTGAATCACGGGCTGGGTGAGCTGGTGGCACAGATGCAGCAACATTGTCAGCAACAGCCGG  >  NC_000913/4141941‑4142126

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑