Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 882 254.4 12957588 98.0% 12698436 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,193,274 A→G V135A (GTA→GCA)  thiC ← ThiC

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,193,2740AG100.0% 752.9 / NA 219V135A (GTA→GCA) thiCThiC
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (93/126);  total (93/126)

Only 100 of 233 total aligned reads displayed.

ATGAATTCCATTTCCGGCGTGATGATGCCCTGGCGGGCGTAGTGCAGTTGGGTGACACGGCGTCCTGCTTTGGCGCGTTTTGGTGTTAGTACGCCGCTAAAACGCAGTTCGTCGAGGCCATCATCTGCCAGCCGCGCTTTAGTGTAATCGGAACTGCGCACGGTAAGTTCTTCGGTATCG  >  NC_000913/4193184‑4193363
                                                                                          |                                                                                         
aTGAATTCCATTTCCGGCGTGATGATGCCCTGGCGGGCGTAGTGCAGTTGGGTGACACGGCGTCCTGCTTTGGCGCGTTTTGGTGTTAGTGcg                                                                                         >  1:4488127/1‑93 (MQ=255)
 tGAATTCCATTTCCGGCGTGATGATGCCCTGGCGGGCGTAGTGCAGTTGGGTGACACGGCGTCCTGCTTTGGCGCGTTTTGGTGTTAGTgcgc                                                                                        >  1:1232854/1‑93 (MQ=255)
 tGAATTCCATTTCCGGCGTGATGATGCCCTGGCGGGCGTAGTGCAGTTGGGTGACACGGCGTCCTGCTTTGGCGCGTTTTGGTGTTAGTgcgc                                                                                        >  1:4436582/1‑93 (MQ=255)
 tGAATTCCATTTCCGGCGTGATGATGCCCTGGCGGGCGTAGTGCAGTTGGGTGACACGGCGTCCTGCTTTGGCGCGTTTTGGTGTTAGTgcgc                                                                                        >  2:5949868/1‑93 (MQ=255)
   aaTTCCATTTCCGGCGTGATGACGCCCTGGCGGGCGTAGTGCAGTTGGGTGACACGGCGTCCTGCTTTGGCGCGTTTTGGTGTTAGTGcgccg                                                                                      >  2:4652834/1‑93 (MQ=255)
     ttCCATTTCCGGCGTGATGATGCCCTGGCGGGCGTAGTGCAGTTGGGTGACACGGCGTCCTGCTTTGGCGCGTTTTGGTGTTAGTGCGCCGCt                                                                                    <  2:1055654/93‑1 (MQ=255)
     ttCCATTTCCGGCGTGATGATGCCCTGGCGGGCGTAGTGCAGTTGGGTGACACGGCGTCCTGCTTTGGCGCGTTTTGGTGTTAGTGCGCCGCt                                                                                    <  1:2978954/93‑1 (MQ=255)
      tCCATTTCCGGCGTGATGATGCCCTGGCGGGCGTAGTGCAGTTGGGTGACACGGCGTCCTGCTTTGGCGCGTTTTGGTGTTAGTGCGCCGCTa                                                                                   >  1:6258843/1‑93 (MQ=255)
      tCCATTTCCGGCGTGATGATGCCCTGGCGGGCGTAGTGCAGTTGGGTGACACGGCGTCCTGCTTTGGCGCGTTTTGGTGTTAGTGCGCCGCTa                                                                                   >  1:1631441/1‑93 (MQ=255)
      tCCATTTCCGGCGTGATGATGCCCTGGCGGGCGTAGTGCAGTTGGGTGACACGGCGTCCTGCTTTGGCGCGTTTTGGTGTTAGTGCGCCGCTa                                                                                   <  2:3564033/93‑1 (MQ=255)
        cATTTCCGGCGTGATGATGCCCTGGCGGGCGTAGTGCAGTTGGGTGACACGGCGTCCTGCTTTGGCGCGTTTTGGTGTTAGTGCGCCGCTaaa                                                                                 <  1:1807364/93‑1 (MQ=255)
        cATTTCCGGCGTGATGATGCCCTGGCGGGCGTAGTGCAGTTGGGTGACACGGCGTCCTGCTTTGGCGCGTTTTGGTGTTAGTGCGCCGCTaaa                                                                                 >  2:688263/1‑93 (MQ=255)
        cATTTCCGGCGTGATGATGCCCTGGCGGGCGTAGTGCAGTTGGGTGACACGGCGTCCTGCTTTGGCGCGTTTTGGTGTTAGTGCGCCGCTaaa                                                                                 <  2:5553615/93‑1 (MQ=255)
         aTTTCCGGCGTGATGATGCCCTGGCGGGCGTAGTGCAGTTGGGTGACACGGCGTCCTGCTTTGGCGCGTTTTGGTGTTAGTGCGCCGCTaaaa                                                                                >  1:1148317/1‑93 (MQ=255)
         aTTTCCGGCGTGATGATGCCCTGGCGGGCGTAGTGCAGTTGGGTGACACGGCGTCCTGCTTTGGCGCGTTTTGGTGTTAGTGCGCCGCTaaaa                                                                                <  2:4957247/93‑1 (MQ=255)
         aTTTCCGGCGTGATGATGCCCTGGCGGGCGTAGTGCAGTTGGGTGACACGGCGTCCTGCTTTGGCGCGTTTTGGTGTTAGTGCGCCGCTaaaa                                                                                <  2:479314/93‑1 (MQ=255)
          tttCCGGCGTGATGATGCCCTGGCGGGCGTAATGCAGTTGGGTGACACGGCGTCCTGCTTTGGCGCGTTTTGGTGTTAGTGCGCCGCTAAAAc                                                                               <  2:2998450/93‑1 (MQ=255)
           ttCCGGCGTGATGATGCCCTGGCGGGCGTAGTGCAGTTGGGTGACACGGCGTCCTGCTTTGGCGCGTTTTGGTGTTAGTGCGCCGCTAAAACg                                                                              <  2:331116/93‑1 (MQ=255)
            tCCGGCGTGATGATGCCCTGGCGGGCGTAGTGCAGTTGGGTGACACGGCGTCCTGCTTTGGCGCGGTTTTGTGTTAGTGCGCCGCTAAAACGc                                                                             >  2:511064/1‑93 (MQ=255)
             ccGGCGTGATGATGCCCTGGCGGGCGTAGTGCAGTTGGGTGACACGGCGTCCTGCTTTGGCGCGTTTTGGTGTTAGTGCGCCGCTAAAACGCa                                                                            <  1:3720770/93‑1 (MQ=255)
             ccGGCGTGATGATGCCCTGGCGGGCGTAGTGCAGTTGGGTGACACGGCGTCCTGCTTTGGCGCGTTTTGGTGTTAGTGCGCCGCTAAAACGCa                                                                            <  1:3547521/93‑1 (MQ=255)
               ggCGTGATGATGCCCTGGCGGGCGTAGTGCAGTTGGGTGACACGGCGTCCTGCTTTGGCGCGTTTTGGTGTTAGTGCGCCGCTAAAACGCAGt                                                                          >  1:2767452/1‑93 (MQ=255)
               ggCGTGATGATGCCCTGGCGGGCGTAGTGCAGTTGGGTGACACGGCGTCCTGCTTTGGCGCGTTTTGGTGTTAGTGCGCCGCTAAAACGCAGt                                                                          <  1:4982753/93‑1 (MQ=255)
                  gTGATGATGCCCTGGCGGGCGTAGTGCAGTTGGGTGACACGGCGTCCTGCTTTGGCGCGCTTTGGTGTTAGTGCGCCGCTAAAACGCAGTtcg                                                                       >  1:3408491/1‑93 (MQ=255)
                   tgatgaTGCCCTGGCGGGCGTAGTGCAGTTGGGTGACACGGCGTCCTGCTTTGGCGCGTTTTGGTGTTAGTGCGCCGCTAAAACGCAGTtcgt                                                                      >  1:3358683/1‑93 (MQ=255)
                   tgatgaTGCCCTGGCGGGCGTAGTGCAGTTGGGTGACACGGCGTCCTGCTTTGGCGCGTTTTGGTGTTAGTGCGCCGCTAAAACGCAGTtcgt                                                                      >  2:4503107/1‑93 (MQ=255)
                   tgatgaTGCCCTGGCGGGCGTAGTGCAGTTGGGTGACACGGCGTCCTGCTTTGGCGCGTTTTGGTGTTAGTGCGCCGCTAAAACGCAGTtcgt                                                                      <  2:2606896/93‑1 (MQ=255)
                   tgatgaTGCCCTGGCGGGCGTAGTGCAGTTGGGTGACACGGCGTCCTGCTTTGGCGCGTTTTGGTGTTAGTGCGCCGCTAAAAAGCAGTtcgt                                                                      >  2:2766976/1‑93 (MQ=255)
                    gatgatGCCCTGGCGGGCGTAGTGCAGTTGGGTGACACGGCGTCCTGCTTTGGCGCGTTTTGGTGTTAGTGCGCCGCTAAAACGCAGTtcgtc                                                                     >  1:5714707/1‑93 (MQ=255)
                     atgatgCCCTGGCGGGCGTAGTGCAGTTGGGTGACACGGCGTCCTGCTTTGGCGCGTTTTGGTGTTAGTGCGCCGCTAAAACGCAGTtcgtcg                                                                    <  1:2651338/93‑1 (MQ=255)
                         tgCCCTGGCGGGCGTAGTGCAGTTGGGTGACACGGCGTCCTGCTTTGGCGCGTTTTGGTGTTAGTGCGCCGCTAAAACGCAGTTCGTCGAGGc                                                                >  2:6333964/1‑93 (MQ=255)
                         tgCCCTGGCGGGCGTAGTGCAGTTGGGTGACACGGCGTCCTGCTTTGGCGCGTTTTGGTGTTAGTGCGCCGCTAAAACGCAGTTCGTCGAGGc                                                                <  1:6295965/93‑1 (MQ=255)
                           cccTGGCGGGCGTAGTGCAGTTGGGTGACACGGCGTCCTGCTTTGGCGCGTTTTGGTGTTAGTGCGCCGCTAAAACGCAGTTCGTCGAGGCca                                                              <  2:1252905/93‑1 (MQ=255)
                            ccTGGCGGGCGTAGTGCAGTTGGGTGACACGGCGTCCTGCTTTGGCGCGTTTTGGTGTTAGTGCGCCGCTAAAACGCAGTTCGTCGAGGCcat                                                             >  2:4114596/1‑93 (MQ=255)
                             cTGGCGGGGGTAGTGCAGGTGGGGGACACGGCGTCCTGCTTTGGCGCGTTTTGGTGTTAGTGCGCCGCTAAAACGCAGTTCGTCGAGGCcatc                                                            >  2:1347555/1‑93 (MQ=255)
                             cTGGCGGGCGTAGTGCAGTTGGGTGACACGGCGTCCTGCTTTGGCGCGTTTTGGTGTTAGTGCGCCGCTAAAACGCAGTTCGTCGAGGCcatc                                                            >  2:2527807/1‑93 (MQ=255)
                             cTGGCGGGCGTAGTGCAGTTGGGTGACACGGCGTCCTGCTTTGGCGCGTTTTGGTGTTAGTGCGCCGCTAAAACGCAGTTCGTCGAGGCcatc                                                            <  2:2480154/93‑1 (MQ=255)
                             cTGGCGGGCGTAGTGCAGTTGGGTGACACGGCGTCCTGCTTTGGCGCGTTTTGGTGTTAGTGCGCCGCTAAAACGCAGTTCGTCGAGGCcatc                                                            >  1:2563801/1‑93 (MQ=255)
                             cTGGCGGGCGTAGTGCAGTTGGGGGACACGGCGTCCTGCTTTGGCGCGTTTTGGGGTTAGTGCGCCGCTAAAACGCAGTTCGTCGAGGCcatc                                                            >  2:3289856/1‑93 (MQ=255)
                              tGGCGGGCGTAGTGCAGTTGGGTGACACGGCGTCCTGCTTTGGCGCGTTTTGGTGTTAGTGCGCCGCTAAAACGCAGTTCGTCGAGGCcatca                                                           <  1:523727/93‑1 (MQ=255)
                                  gggcgTAGTGCAGTTGGGTGACACGGCGGCCTGCTTTGGCGCGTTTTGGTGTTAGTGCGCCGCTAAAACGCAGTTCGTCGAGGCCATCATCTg                                                       <  1:2102610/93‑1 (MQ=255)
                                   ggcgTAGTGCAGTTGGGTGACACGGCGTCCTGCTTTGGCGCGTTTTGGTGTTAGTGCGCCGCTAAAACGCAGTTCGTCGAGGCCATCATCTGc                                                      >  2:1801271/1‑93 (MQ=255)
                                   ggcgTAGTGCAGTTGGGTGACACGGCGTCCTGCTTTGGCGCGTTTTGGTGTTAGTGCGCCGCTAAAACGCAGTTCGTCGAGGCCATCATCTGc                                                      >  2:1452029/1‑93 (MQ=255)
                                   ggcgTAGTGCAGTTGGGTGACACGGCGTCCTGCTTTGGCGCGTTTTGGTGTTAGTGCGCCGCTAAAACGCAGTTCGTCGAGGCCATCATCTGc                                                      >  1:4368834/1‑93 (MQ=255)
                                    gcgTAGTGCAGTTGGGTGACACGGCGTCCTGCTTTGGCGCGTTTTGGTGTTAGTGCGCCGCTAAAACGCAGTTCGTCGAGGCCATCATCTGcc                                                     >  2:448418/1‑93 (MQ=255)
                                    gcgTAGTGCAGTTGGGTGACACGGCGTCCTGCTTTGGCGCGTTTTGGTGTTAGTGCGCCGCTAAAACGCAGTTCGTCGAGGCCATCATCTGcc                                                     <  2:1870294/93‑1 (MQ=255)
                                    gcgTAGTGCAGTTGGGTGACACGGCGTCCTGCTTTGGCGCGTTTTGGTGTTAGTGCGCCGCTAAAACGCAGTTCGTCGAGGCCATCATCTGcc                                                     <  2:1617100/93‑1 (MQ=255)
                                    gcgTAGTGCAGTTGGGTGACACGGCGTCCTGCTTTGGCGCGTTTTGGTGTTAGTGCGCCGCTAAAACGCAGTTCGTCGAGGCCATCATCTGcc                                                     <  2:5020169/93‑1 (MQ=255)
                                    gcgTAGTGCAGTTGGGTGACACGGCGTCCTGCTTTGGCGCGTTTTGGTGTTAGTGCGCCGCTAAAACGCAGTTCGTCGAGGCCATCATCTGcc                                                     >  2:4739628/1‑93 (MQ=255)
                                    gcgTAGTGCAGTTGGGTGACACGGCGTCCTGCTTTGGCGCGTTTTGGTGTTAGTGCGCCGCTAAAACGCAGTTCGTCGAGGCCATCATCTGcc                                                     >  2:5695326/1‑93 (MQ=255)
                                        aGTGCAGTTGGGTGACACGGCGTCCTGCTTTGGCGCGTTTTGGTGTTAGTGCGCCGCTAAAACGCAGTTCGTCGAGGCCATCATCTGCCAGcc                                                 <  2:3611854/93‑1 (MQ=255)
                                         gttcAGTTTGGTGACACGGCGTCCTGCTTTTGCGCGTTTTGGTGTTAGTGCGCCGCTAAAACGCAGTTCGTCGAGGCCATCATCTGCCAGCcg                                                <  1:1308478/90‑1 (MQ=255)
                                          tGCAGTTGGGTGACACGGCGTCCTGCTTTGGCGCGTTTTGGTGTTAGTGCGCCGCTAAAACGCAGTTCGTCGAGGCCATCATCTGCCAGCcgc                                               <  2:5400133/93‑1 (MQ=255)
                                          tGCAGTTGGGTGACACGGCGTCCTGCTTTGGCGCGTTTTGGTGTTAGTGCGCCGCTAAAACGCAGTTCGTCGAGGCCATCATCTGCCAGCcgc                                               <  1:295634/93‑1 (MQ=255)
                                          tGCAGTTGGGTGACACGGCGTCCTGCTTTGGCGCGTTTTGGTGTTAGTGCGCCGCTAAAACGCAGTTCGTCGAGGCCATCATCTGCCAGCcgc                                               >  1:5903341/1‑93 (MQ=255)
                                            cAGTTGGGTGACACGGCGTCGTGCTTTGGCGCGTTTTGGTGTTAGTGCGCCGCTAAAACGCAGTTCGTCGAGGCCATCATCTGCCAGCCgcgc                                             <  2:3545850/93‑1 (MQ=255)
                                             aGTTGGGTGACCCGGCGTCCTGCTTTGGCGCGTTTTGGTGTTAGTGCGCCGCTAAAACGCAGTTCGTCGAGGCCATCATCTGCCAGCCGCGCt                                            <  1:4293339/93‑1 (MQ=255)
                                             aGTTGGGTGACACGGCGTCCTGCTTTGGCGCGTTTTGGTGTTAGTGCGCCGCTAAAACGCAGTTCGTCGAGGCCATCATCTGCCAGCCGCGCt                                            <  1:1448611/93‑1 (MQ=255)
                                              gTTGGGTGACACGGCGTCCTGCTTTGGCGCGTTTTGGTGTTAGTGCGCCGCTAAAACGCAGTTCGTCGAGGCCATCATCTGCCAGCCGCGCtt                                           >  1:3186414/1‑93 (MQ=255)
                                                tGGGTGACACGGCGTCCTGCTTTGGCGCGTTTTGGTGTTAGTGCGCCGCTAAAACGCAGTTCGTCGAGGCCATCATCTGCCAGCCGCGCTTTa                                         <  1:3953508/93‑1 (MQ=255)
                                                   gTGACACGGCGTCCTGCTTTGGCGCGTTTTGGTGTTAGTGCGCCGCTAAAACGCAGTTCGTCGAGGCCATCATCTGCCAGCCGCGCTTTAgtg                                      <  2:2039349/93‑1 (MQ=255)
                                                        acGGCGTCCTGCTTTGGCGCGTTTTGGTGTTAGTGCGCCGCTAAAACGCAGTTCGTCGAGGCCATCATCTGCCAGCCGCGCTTTAGTGTAATc                                 <  2:1495571/93‑1 (MQ=255)
                                                        acGGCGTCCTGCTTTGGCGCGTTTTGGTGTTAGTGCGCCGCTAAAACGCAGTTCGTCGAGGCCATCATCTGCCAGCCGCGCTTTAGTGTAATc                                 >  2:2302646/1‑93 (MQ=255)
                                                           gCGTCCTGCTTTGGCGCGTTTTGGTGTTAGTGCGCCGCTAAAACGCAGTTCGTCGAGGCCATCATCTGCCAGCCGCGCTTTAGTGTAATCGGa                              <  1:5309542/93‑1 (MQ=255)
                                                            cGTCCTGCTTTGGCGCGTTTTGGTGTTAGTGCGCCGCTAAAACGCAGTTCGTCGAGGCCATCATCTGCCAGCCGCGCTTTAGTGTAATCGGaa                             >  1:4743223/1‑93 (MQ=255)
                                                             gTCCTGCTTTGGCGCGTTTTGGTGTTAGTGCGCCGCTAAAACGCAGTTCGTCGAGGCCATCATCTGCCAGCCGCGCTTTAGTGTAATCGGAAc                            <  2:6292463/93‑1 (MQ=255)
                                                             gTCCTGCTTTGGCGCGTTTTGGTGTTAGTGCGCCGCTAAAACGCAGTTCGTCGAGGCCATCATCTGCCAGCCGCGCTTTAGTGTAATCGGAAc                            >  1:5418049/1‑93 (MQ=255)
                                                             gTCCTGCTTTGGCGCGTTTTGGTGTTAGTGCGCCGCTAAAACGCAGTTCGTCGAGGCCATCATCTGCCAGCCGCGCTTTAGTGTAATCGGAAc                            <  2:3370879/93‑1 (MQ=255)
                                                              tCCTGCTTTGGCGCGTTTTGGTGTTAGTGCGCCGCTAAAACGCAGTTCGTCGAGGCCATCATCTGCCAGCCGCGCTTTAGTGTAATCGGGACt                           >  2:5463819/1‑93 (MQ=255)
                                                              tCCTGCTTTGGCGCGTTTTGGTGTTAGTGCGCCGCTAAAACGCAGTTCGTCGAGGCCATCATCTGCCAGCCGCGCTTTAGTGTAATCGGAACt                           >  1:4273155/1‑93 (MQ=255)
                                                              tCCTGCTTTGGCGCGTTTTGGTGTTAGTGCGCCGCTAAAACGCAGTTCGTCGAGGCCATCATCTGCCAGCCGCGCTTTAGTGTAATCGGAACt                           <  1:2805986/93‑1 (MQ=255)
                                                              tCCTGCTTTGGCGCGTTTTGGTGTTAGTGCGCCGCTAAAACGCAGTTCGTCGAGGCCATCATCTGCCAGCCGCGCTTTAGTGTAATCGGAACt                           >  1:5779202/1‑93 (MQ=255)
                                                               ccTGCTTTGGCGCGTTTTGGTGTTAGTGCGCCGCTAAAACGCAGTTCGTCGAGGCCATCATCTGCCAGCCGCGCTTTAGTGTAATCGGAACTg                          <  1:2520631/93‑1 (MQ=255)
                                                                cTGCTTTGGCGCGTTTTGGTGTTAGTGCGCCGCTAAAACGCAGTTCGTCGAGGCCATCATCTGCCAGCCGCGCTTTAGTGTAATCGGAACTgc                         >  1:5717733/1‑93 (MQ=255)
                                                                  gCTTTGGCGCGTTTTGGTGTTAGTGCGCCGCTAAAACGCAGTTCGTCGAGGCCATCATCTGCCAGCCGCGCTTTAGTGTAATCGGAACTgcgc                       >  2:2149943/1‑93 (MQ=255)
                                                                  gCTTTGGCGCGTTTTGGTGTTAGTGCGCCGCTAAAACGCAGTTCGTCGAGGCCATCATCTGCCAGCCGCGCTTTAGTGTAATCGGAACTgcgc                       >  2:5763538/1‑93 (MQ=255)
                                                                   cTTTGGCGCGTTTTGGTGTTAGTGCGCCGCTAAAACGCAGTTCGTCGAGGCCATCATCTGCCTGCCGCGCTTTAGTGTAATCGGAACTGCGCa                      >  2:2563759/1‑93 (MQ=255)
                                                                   cTTTGGCGCGTTTTGGTGTTAGTGCGCCGCTAAAACGCAGTTCGTCGAGGCCATCATCTGCCAGCCGCGCTTTAGTGTAATCGGAACTGCGCa                      <  1:6211363/93‑1 (MQ=255)
                                                                   cTTTGGCGCGTTTTGGTGTTAGTGCGCCGCTAAAACGCAGTTCGTCGAGGCCATCATCTGCCAGCCGCGCTTTAGTGTAATCGGAACTGCGCa                      <  1:643080/93‑1 (MQ=255)
                                                                   cTTTGGCGCGTTTTGGTGTTAGTGCGCCGCTAAAACGCAGTTCGTCGAGGCCATCATCTGCCAGCCGCGCTTTAGTGTAATCGGAACTGCGCa                      <  1:852995/93‑1 (MQ=255)
                                                                      tGGCGCGTTTTGGTGTTAGTGCGCCGCTAAAACGCAGTTCGTCGAGGCCATCATCTGCCAGCCGCGCTTTAGTGTAATCGGAACTGCGCACgg                   <  2:3947990/93‑1 (MQ=255)
                                                                       ggCGCGTTTTGGTGTTAGTGCGCCGCTAAAACGCAGTTCGTCGAGGCCATCATCTGCCAGCCGCGCTTTAGTGTAATCGGAACTGCGCACGGt                  >  1:899286/1‑93 (MQ=255)
                                                                         cgcgTTTTGGTGTTAGTGCGCCGCTAAAACGCAGTTCGTCGAGGCCATCATCTGCCAGCCGCGCTTTAGTGTAATCGGAACTGCGCACGGTaa                <  2:3363521/93‑1 (MQ=255)
                                                                          gcgTTTTGGTGTTAGTGCGCCGCTAAAACGCAGTTCGTCGAGGCCATCATCTGCCAGCCGCGCTTTAGTGTAATCGGAACTGCGCACGGTAAg               <  2:802552/93‑1 (MQ=255)
                                                                           cgTTTTGGTGTTAGTGCGCCGCTAAAACGCAGTTCGTCGAGGCCATCATCTGCCAGCCGCGCTTTAGTGTAATCGGAACTGCGCACGGTAAGt              >  2:4616831/1‑93 (MQ=255)
                                                                           cgTTTTGGTGTTAGTGCGCCGCTAAAACGCAGTTCGTCGAGGCCATCATCTGCCAGCCGCGCTTTAGTGTAATCGGAACTGCGCACGGTAAGt              >  2:4077695/1‑93 (MQ=255)
                                                                            gTTTTGGTGTTAGTGCGCCGCTAAAACGCAGTTCGTCGAGGCCATCATCTGCCAGCCGCGCTTTAGTGTAATCGGAACTGCGCACGGTAAGtt             <  1:1046086/93‑1 (MQ=255)
                                                                             ttttGGTGTTAGTGCGCCGCTAAAACGCAGTTCGTCGAGGCCATCATCTGCCAGCCGCGCTTTAGTGTAATCGGAACTGCGCACGGTAAGttc            <  2:5311120/93‑1 (MQ=255)
                                                                             ttttGGTGTTAGTGCGCCGCTAAAACGCAGTGCGTCGAGGCCATCATCTGCCAGCCGCGCTTTAGTGTAATCGGAACTGCGCACGGTAAGttc            <  2:2903233/93‑1 (MQ=255)
                                                                              tttGGTGTTAGTGCGCCGCTAAAACGCAGTTCGTCGAGGCCATCATCTGCTAGCCGCGCTTTAGTGTAATCGGAACTGCGCACGGTAAGttct           <  1:3456216/93‑1 (MQ=255)
                                                                              tttGGTGTTAGTGCGCCGCTAAAACGCAGTTCGTCGAGGCCATCATCTGCCAGCCGCGCTTTAGTGTAATCGGAACTGCGCACGGTAAGttct           <  1:825345/93‑1 (MQ=255)
                                                                              tttGGTGTTAGTGCGCCGCTAAAACGCAGTTCGTCGAGGCCATCATCTGCCAGCCGCGCTTTAGTGTAATCGGAACTGCGCACGGTAAGttat           <  1:2475526/93‑3 (MQ=255)
                                                                               ttGGTGTTAGTGCGCCGCTAAAACGCAGTTCGTCGAGGCCATCATCTGCCAGCCGCGCTTTAGTGTAATCGGAACTGCGCACGGTAAGttctt          <  2:2750739/93‑1 (MQ=255)
                                                                                tGGTGTTAGTGCGCCGCTAAAACGCAGTTCGTCGAGGCCATCATCTGCCAGCCGCGCTTTAGTGTAATCGGAACTGCGCACGGTAAGttcttc         <  1:6037659/93‑1 (MQ=255)
                                                                                tGGTGTTAGTGCGCCGCTAAAACGCAGTTCGTCGAGGCCATCATCTGCCAGCCGCGCTTTAGTGTAATCGGAACTGCGCACGGTAAGttcttc         <  2:6380364/93‑1 (MQ=255)
                                                                                    gtTAGTGCGCCGCTAAAACGCAGTTCGTCGAGGCCATCATCTGCCAGCCGCGCTTTAGTGTAATCGGAACTGCGCACGGGAAGTTCTTCGGTa     >  1:4570377/1‑93 (MQ=255)
                                                                                     ttAGTGCGCCGCTAAAACGCAGTTCGTCGAGGCCATCATCTGCCAGCCGCGCTTTAGTGTAATCGGAACTGCGCACGGTAAGTTCTTCGGTAt    >  2:110963/1‑93 (MQ=255)
                                                                                     ttAGTGCGCCGCTAAAACGCAGTTCGTCGAGGCCATCATCTGCCAGCCGCGCTTTAGTGTAATCGGAACTGCGCACGGTAAGTTCTTCGGTAt    <  1:5128280/93‑1 (MQ=255)
                                                                                     ttAGTGCGCCGCTAAAACGCAGTTCGTCGAGGCCATCATCTGCCAGCCGCGCTTTAGTGTAATCGGAACTGCGCACGGTAAGTTCTTCGGTAt    >  1:2051889/1‑93 (MQ=255)
                                                                                       aGTGCGCCGCTAAAACGCAGTTCGTCGAGGCCATCATCTGCCAGCCGCGCTTTAGTGTAATCGGAACTGCGCACGGTAAGTTCTTCGGTATcg  >  1:2202060/1‑93 (MQ=255)
                                                                                          |                                                                                         
ATGAATTCCATTTCCGGCGTGATGATGCCCTGGCGGGCGTAGTGCAGTTGGGTGACACGGCGTCCTGCTTTGGCGCGTTTTGGTGTTAGTACGCCGCTAAAACGCAGTTCGTCGAGGCCATCATCTGCCAGCCGCGCTTTAGTGTAATCGGAACTGCGCACGGTAAGTTCTTCGGTATCG  >  NC_000913/4193184‑4193363

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GCGATGAATTCCATTTCCGGCGTGATGATGCCCTGGCGGGCGTAGTGCAGTTGGGTGACACGGCGTCCTGCTTTGGCGCGTTTTGGTGTTAGTACGCCGCTAAAACGCAGTTCGTCGAGGCCATCATCTGCCAGCCGCGCTTTAGTGTAATCGGAACTGCGCACGGTAAGTTCTTCGGTATCG  >  NC_000913/4193181‑4193363
                                                                                             |                                                                                         
GCGATGAATTCCATTTCCGGCGTGATGATGCCCTGGCGGGCGTAGTGCAGTTGGGTGACACGGCGTCCTGCTTTGGCGCGTTTTGGTGTTAGTG                                                                                           <  SRR4302537.568801/94‑1 (MQ=60)
 CGATGAATTCCATTTCCGGCGTGATGATGCCCTGGCGGGCGTAGTGCAGTTGGGTGACACGGCGTCCTGCTTTGGCGCGTTTTGGTGTTAGTGC                                                                                          >  SRR4302537.5500815/1‑94 (MQ=60)
  GATGAATTCCATTTCCGGCGTGATGATGCCCTGGCGGGCGTAGTGCAGTTGGGTGACACGGCGTCCTGCTTTGGCGCGTTTTGGTGTTAGTGCG                                                                                         >  SRR4302537.4519174/1‑94 (MQ=60)
  GATGAATTCCATTTCCGGCGTGATGATGCCCTGGCGGGCGTAGTGCAGTTAGGTGACACGGCGTCCTGCTTTGGCGCGTTTTGGTGTTAGTGCG                                                                                         >  SRR4302537.3727536/1‑94 (MQ=60)
   ATGAATTCCATTTCCGGCGTGATGATGCCCTGGCGGGCGTCGTGCAGTTGGGTGACACGGAGTCCTGCCTTGGCGCGCTTTGGCGTTAGTGCGC                                                                                        >  SRR4302537.4451782/1‑94 (MQ=60)
   ATGAATTCCATTTCCGGCGTGATGATGCCCTGGCGGGCGTAGTGCAGTTGGGTGACACGGCGTCCTGCTTTGGCGCGTTTTGGTGTTAGTGCGC                                                                                        >  SRR4302537.1241430/1‑94 (MQ=60)
   ATGAATTCCATTTCCGGCGTGATGATGCCCTGGCGGGCGTAGTGCAGTTGGGTGACACGGCGTCCTGCTTTGGCGCGTTTTGGTGTTAGTGCGC                                                                                        >  SRR4302537.4467216/1‑94 (MQ=60)
     GAATTCCATTTCCGGCGTGATGATGCCCTGGCGGGCGTAGTGCAGTTGGGTGACACGGCGTCCTGCTTTGGCGCGTTTTGGTGTTAGTGCGCCG                                                                                      <  SRR4302537.5956535/94‑1 (MQ=60)
     GAATTCCATTTCCGGCGTGATGATGCCCTGGCGGGCGTAGTGCAGTTGGGTGACACGGCGTCCTGCTTTGGCGCGTTTTGGTGTTAGTGCGCCG                                                                                      >  SRR4302537.6296949/1‑94 (MQ=60)
      AATTCCATTTCCGGCGTGATGATGCCCTGGCGGGCGTAGTGCAGTTGGGTGACACGGCGTCCTGCTTTGGCGCGTTTTGGTGTTAGTGCGCCGC                                                                                     >  SRR4302537.3566929/1‑94 (MQ=60)
      AATTCCATTTCCGGCGTGATGATGCCCTGGCGGGCGTAGTGCAGTTGGGTGACACGGCGTCCTGCTTTGGCGCGTTTTGGTGTTAGTGCGCCGC                                                                                     >  SRR4302537.864814/1‑94 (MQ=60)
        TTCCATTTCCGGCGTGATGATGCCCTGGCGGGCGTAGTGCAGTTGGGTGACACGGCGTCCTGCTTTGGCGCGTTTTGGTGTTAGTGCGCCGCTA                                                                                   >  SRR4302537.6301821/1‑94 (MQ=60)
        TTCCATTTCCGGCGTGATGATGCCCTGGCGGGCGTAGTGCAGTTGGGTGACACGGCGTCCTGCTTTGGCGCGTTTTGGTGTTAGTGCGCCGCTA                                                                                   <  SRR4302537.2999795/94‑1 (MQ=60)
        TTCCATTTCCGGCGTGATGATGCCCTGGCGGGCGTAGTGCAGTTGGGTGACACGGCGTCCTGCTTTGGCGCGTTTTGGTGTTAGTGCGCCGCTA                                                                                   >  SRR4302537.1642869/1‑94 (MQ=60)
         TCCATTTCCGGCGTGATGATGCCCTGGCGGGCGTAGTGCAGTTGGGTGACACGGCGTCCTGCTTTGGCGCGTTTTGGTGTTAGTGCGCCGCTAA                                                                                  >  SRR4302537.5439110/1‑94 (MQ=60)
          CCATTTCCGGCGTGATGATGCCCTGGCGGGCGTAGTGCAGTTGGGTGACACGGCGTCCTGCTTTGGCGCGTTTTGGTGTTAGTGCGCCGCTAAA                                                                                 <  SRR4302537.4617256/94‑1 (MQ=60)
          CCATTTCCGGCGTGATGATGCCCTGGCGGGCGTAGTGCAGTTGGGTGACACGGCGTCCTGCTTTGGCGCGTTTTGGTGTTAGTGCGCCGCTAAA                                                                                 >  SRR4302537.4671659/1‑94 (MQ=60)
           CATTTCCGGCGTGATGATGCCCTGGCGGGCGTAGTGCAGTTGGGTGACACGGCGTCCTGCTTTGGCGCGTTTTGGTGTTAGTGCGCCGCTAAAA                                                                                <  SRR4302537.1819973/94‑1 (MQ=60)
           CATTTCCGGCGTGATGATGCCCTGGCGGGCGTAGTGCAGTTGGGTGACACGGCGTCCTGCTTTGGCGCGTTTTGGTGTTAGTGCGCCGCTAAAA                                                                                >  SRR4302537.2130/1‑94 (MQ=60)
           CATTTCCGGCGTGATGATGCCCTGGCGGGCGTAGTGCAGTTGGGTGACACGGCGTCCTGCTTTGGCGCGTTTTGGTGTTAGTGCGCCGCTAAAA                                                                                >  SRR4302537.1156300/1‑94 (MQ=60)
            ATTTCCGGCGTGATGATGCCCTGGCGGGCGTAGTGCAGTTGGGTGACACGGCGTCCTGCTTTGGCGCGTTTTGGTGTTAGTGCGCCGCTAAAAC                                                                               <  SRR4302537.3950569/94‑1 (MQ=60)
            ATTTCCGGCGTGATGATGCCCTGGCGGGCGTAGTGCAGTTGGGTGACACGGCGTCCTGCTTTGGCGCGTTTTGGTGTTAGTGCGCCGCTAAAAC                                                                               >  SRR4302537.4397657/1‑94 (MQ=60)
            ATTTCCGGCGTGATGATGCCCTGGCGGGCGTAGTGCAGTTGGGTGACACGGCGTCCTGCTTTGGCGCGTTTTGGTGTTAGTGCGCCGCTAAAAC                                                                               <  SRR4302537.1521214/94‑1 (MQ=60)
               TCCGGCGTGATGATGCCCTGGCGGGCGTAGTGCAGTTGGGTGACACGGCGTCCTGCTTTGGCGCGTTTTGGTGTTAGTGCGCCGCTAAAACGCA                                                                            <  SRR4302537.2565124/94‑1 (MQ=60)
                CCGGCGTGATGATGCCCTGGCGGGCGTAGTGCAGTTGGGTGACACGGCGTCCTGCTTTGGCGCGTTTTGGTGTTAGTGCGCCGCTAAAACGCAG                                                                           <  SRR4302537.3746912/94‑1 (MQ=60)
                CCGGCGTGATGATGCCCTGGCGGGCGTAGTGCAGTTGGGTGACACGGCGTCCTGCTTTGGCGCGTTTTGGTGTTAGTGCGCCGCTAAAACGCAG                                                                           <  SRR4302537.3572581/94‑1 (MQ=60)
                CCGGCGTGATGATGCCCTGGCGGGCGTAGTGCAGTTGGGTGACACGGCGTCCTGCTTTGGCGCGTTTTGGTGTTAGTGCGCCGCTAAAACGCAG                                                                           >  SRR4302537.619539/1‑94 (MQ=60)
                 CGGCGTGATGATGCCCTGGCGGGCGTAGTGCAGTTGGGTGACACGGCGTCCTGCTTTGGCGCGTTTTGGTGTTAGTGCGCCGCTAAAACGCAGT                                                                          >  SRR4302537.2786795/1‑94 (MQ=60)
                  GGCGTGATGATGCCCTGGCGGGCGTAGTGCAGTTGGGTGACACGGCGTCCTGCTTTGGCGCGTTTTGGTGTTAGTGCGCCGCTAAAACGCAGTT                                                                         <  SRR4302537.5017095/94‑1 (MQ=60)
                    CGTGATGATGCCCTGGCGGGCGTAGTGCAGTTGGGTGACACGGCGTCCTGCTTTGGCGCGTTTTGGTGTTAGTGCGCCGCTAAAACGCAGTTCG                                                                       >  SRR4302537.2779657/1‑94 (MQ=60)
                    CGTGATGATGCCCTGGCGGGCGTAGTGCAGTTGGGTGACACGGCGTCCTGCTTTGGCGCGTTTTGGTGTTAGTGCGCCGCTAAAACGCAGTTCG                                                                       <  SRR4302537.2640319/94‑1 (MQ=60)
                    CGTGATGATGCCCTGGCGGGCGTAGTGCAGTTGGGTGACACGGCGTCCTGCTTTGGCGCGCTTTGGTGTTAGTGCGCCGCTAAAACGCAGTTCG                                                                       >  SRR4302537.3432640/1‑94 (MQ=60)
                     GTGATGATGCCCTGGCGGGCGTAGTGCAGTTGGGTGACACGGCGTCCTGCTTTGGCGCGTTTTGGTGTTAGTGCGCCGCTAAAACGCAGTTCGT                                                                      >  SRR4302537.3382474/1‑94 (MQ=60)
                     GGGATGATGCCCTGGCGGGCGGAGTGCAGTTGGGTGACACGGCGTCCTGCTTTGGCGCGTTTTGGTGGTAGTGCGCCGCTAAAACGCAGTTCGT                                                                      >  SRR4302537.2594188/1‑94 (MQ=60)
                      TGATGATGCCCTGGCGGGCGTAGTGCAGTTGGGTGACACGGCGTCCTGCTTTGGCGCGTTTTGGTGTTAGTGCGCCGCTAAAACGCAGTTCGTC                                                                     <  SRR4302537.854860/94‑1 (MQ=60)
                      TGATGATGCCCTGGCGGGCGTAGTGCAGTTGGGTGACACGGCGTCCTGCTTTGGCGCGTTTTGGTGTTAGTGCGCCGCTAAAACGCAGTTCGTC                                                                     >  SRR4302537.5753816/1‑94 (MQ=60)
                        ATGATGCCCTGGCGGGCGTAGTGCAGTTGGGTGACACGGCGTCCTGCTTTGGCGCGTTTTGGTGTTAGTGCGCCGCTAAAACGCAGTTCGTCGA                                                                   >  SRR4302537.676065/1‑94 (MQ=60)
                        ATGATGCCCTGGCGGGCGTAGTGCAGTTGGGTGACACGGCGTCCTGCTTTGGCGCGTTTTGGTGTTAGTGCGCCGCTAAAACGCAGTTCGTCGA                                                                   <  SRR4302537.2669882/94‑1 (MQ=60)
                           ATGCCCTGGCGGGCGTAGTGCAGTTGGGTGACACGGCGTCCTGCTTTGGCGCGTTTTGGTGTTAGTGCGCCGCTAAAACGCAGTTCGTCGAGGC                                                                >  SRR4302537.141909/1‑94 (MQ=60)
                            TGCCCTGGCGGGCGTAGTGCAGTTGGGTGACACGGCGTCCTGCTTTGGCGCGTTTTGGTGTTAGTGCGCCGCTAAAACGCAGTTCGTCGAGGCC                                                               <  SRR4302537.6339092/94‑1 (MQ=60)
                              CCCTGGCGGGCGTAGTGCAGTTGGGTGACACGGCGTCCTGCTTTGGCGCGTTTTGGTGTTAGTGCGCCGCTAAAACGCAGTTCGTCGAGGCCAT                                                             <  SRR4302537.4596128/94‑1 (MQ=60)
                               CCTGGCGGGCGTAGTGCAGTTGGGTGACACGGCGTCCTGCTTTGGCGCGTTTTGGTGTTAGTGCGCCGCTAAAACGCAGTTCGTCGAGGCCATC                                                            >  SRR4302537.2094740/1‑94 (MQ=60)
                               CCTGGCGGGCGTAGTGCAGTTGGGTGACACGGCGTCCTGCTTTGGCGCGTTTTGGTGTTAGTGCGCCGCTAAAACGCAGTTCGTCGAGGCCATC                                                            >  SRR4302537.2581764/1‑94 (MQ=60)
                                CTGGCGGGCGTAGTGCAGTTGGGTGACACGGCGTCCTGCTTTGGCGCGGTTTGGTGTTAGTGCGCCGCTAAAACGCAGTTCGTCGAGGCCATCA                                                           >  SRR4302537.3761125/1‑94 (MQ=60)
                                 TGGCGGGCGTAGTGCAGTTGGGTGACACGGCGTCCTGCTTTGGCGCGTTTTGGTGTTAGTGCGCCGCTAAAACGCAGTTCGTCGAGGCCATCAT                                                          <  SRR4302537.527321/94‑1 (MQ=60)
                                 TGGCGGGCGTAGTGCAGTTGGGTGACACGGCGTCCTGCTTTGGCGCGTTTTGGTGTTAGTGCGCCGCTAAAACGCAGTTCGTCGAGGCCATCAT                                                          <  SRR4302537.502619/94‑1 (MQ=60)
                                   GCGGGCGTAGTGCAGTTGGGTGACACGGCGTCCTGCTTTGGCGCGTTTTGGTGTTAGTGCGCCGCTAAAACGCAGTTCGTCGAGGCCATCATCT                                                        <  SRR4302537.2496639/94‑1 (MQ=60)
                                     GGGCGTAGTGCAGTTGGGTGACACGGCGTCCTGCTTTGGCGCGTTTTGGTGTTAGTGCGCCGCTAAAACGCAGTTCGTCGAGGCCATCATCTGC                                                      >  SRR4302537.4399028/1‑94 (MQ=60)
                                     GGGCGTAGTGCAGTTGGGTGACACGGCGGCCTGCTTTGGCGCGTTTTGGTGTTAGTGCGCCGCTAAAACGCAGTTCGTCGAGGCCATCATCTGC                                                      <  SRR4302537.2117436/94‑1 (MQ=60)
                                       GCGTAGTGCAGTTGGGTGACACGGCGTCCTGCTTTGGCGCGTTTTGGTGTTAGTGCGCCGCTAAAACGCAGTTCGTCGAGGCCATCATCTGCCA                                                    <  SRR4302537.1069013/94‑1 (MQ=60)
                                         GTAGTGCAGTTGGGTGACACGGCGTCCTGCTTTGGCGCGTTTTGGTGTTAGTGCGCCGCTAAAACGCAGTTCGTCGAGGCCATCATCTGCCAGC                                                  <  SRR4302537.6277723/94‑1 (MQ=60)
                                         GTAGTGCAGTTGGGTGACACGGCGTCCTGCTTTGGCGCGTTTTGGTGTTAGTGCGCCGCTAAAACGCAGTTCGTCGAGGCCATCATCTGCCAGC                                                  >  SRR4302537.4311274/1‑94 (MQ=60)
                                           AGTGCAGTTGGGTGACACGGCGTCCTGCTTTGGCGCGTTTTGGTGTTAGTGCGCCGCTAAAACGCAGTTCGTCGAGGCCATCATCTGCCAGCCG                                                >  SRR4302537.2443326/1‑94 (MQ=60)
                                            GTTCAGTTTGGTGACACGGCGTCCTGCTTTTGCGCGTTTTGGTGTTAGTGCGCCGCTAAAACGCAGTTCGTCGAGGCCATCATCTGCCAGCCGC                                               <  SRR4302537.1317586/94‑1 (MQ=60)
                                            GTGCAGTTGGGTGACACGGCGTCCTGCTTTGGCGCGTTTTGGTGTTAGTGCGCCGCTAAAACGCAGTTCGTCGAGGCCATCATCTGCCAGCCGC                                               >  SRR4302537.5943854/1‑94 (MQ=60)
                                             TGCAGTTGGGTGACACGGCGTCCTGCTTTGGCGCGTTTTGGTGTTAGTGCGCCGCTAAAACGCAGTTCGTCGAGGCCATCATCTGCCAGCCGCG                                              <  SRR4302537.297696/94‑1 (MQ=60)
                                               CAGTTGGGTGACACGGCGTCCTGCTTTGGCGCGTTTTGGTGTTAGTGCGCCGCTAAAACGCAGTTCGTCGAGGCCATCATCTGCCAGCCGCGCT                                            <  SRR4302537.5237085/94‑1 (MQ=60)
                                               CAGTTGGGTGACACGGCGTCCTGCTTTGGCGCGTTTTGGTGTTAGTGCGCCGCTAAAACGCAGTTCGTCGAGGCCATCATCTGCCAGCCGCGCT                                            >  SRR4302537.533751/1‑94 (MQ=60)
                                               CAGTTGGGTGACACGGCGTCCTGCTTTGGCGCGTTTTGGTGTTAGTGCGCCGCTAAAACGCAGTTCGTCGAGGCCATCATCTGCCAGCCGCGCT                                            <  SRR4302537.298538/94‑1 (MQ=60)
                                                AGTTGGGTGACCCGGCGTCCTGCTTTGGCGCGTTTTGGTGTTAGTGCGCCGCTAAAACGCAGTTCGTCGAGGCCATCATCTGCCAGCCGCGCTT                                           <  SRR4302537.4323181/94‑1 (MQ=60)
                                                AGTTGGGTGACACGGCGTCCTGCTTTGGCGCGTTTTGGTGTTAGTGCGCCGCTAAAACGCAGTTCGTCGAGGCCATCATCTGCCAGCCGCGCTT                                           <  SRR4302537.1458704/94‑1 (MQ=60)
                                                AGTTGGGTGACACGGCGTCCTGCTTTGGCGCGTTTTGGTGTTAGTGCGCCGCTAAAACGCAGTTCGTCGAGGCCATCATCTGCCAGCCGCGCTT                                           >  SRR4302537.3208829/1‑94 (MQ=60)
                                                  TTGGGTGACACGGCGTCCTGCTTTGGCGCGTTTTGGTGTTAGTGCGCCGCTAAAACGCAGTTCGTCGAGGCCATCATCTGCCAGCCGCGCTTTA                                         <  SRR4302537.4083698/94‑1 (MQ=60)
                                                   TGGGTGACACGGCGTCCTGCTTTGGCGCGTTTTGGTGTTAGTGCGCCGCTAAAACGCAGTTCGTCGAGGCCATCATCTGCCAGCCGCGCTTTAG                                        <  SRR4302537.5682593/94‑1 (MQ=60)
                                                   TGGGTGACACGGCGTCCTGCTTTGGCGCGTTTTGGTGTTAGTGCGCCGCTAAAACGCAGTTCGTCGAGGCCATCATCTGCCAGCCGCGCTTTAG                                        <  SRR4302537.3981162/94‑1 (MQ=60)
                                                   TGGGTGACACGGCGTCCTGCTTTGGCGCGTTTTGGTGTTAGTGCGCCGCTAAAACGCAGTTCGTCGAGGCCATCATCTGCCAGCCGCGCTTTAG                                        <  SRR4302537.1652451/94‑1 (MQ=60)
                                                    GGGTGACACGGCGTCCTGCTTTGGCGCGTTTTGGTGTTAGTGCGCCGCTAAAACGCAGTTCGTCGAGGCCATCATCTGCCAGCCGCGCTTTAGT                                       >  SRR4302537.2852012/1‑94 (MQ=60)
                                                      GTGACACGGCGTCCTGCTTTGGCGCGTTTTGGTGTTAGTGCGCCGCTAAAACGCAGTTCGTCGAGGCCATCATCTGCCAGCCGCGCTTTAGTGT                                     <  SRR4302537.634900/94‑1 (MQ=60)
                                                          CACGGCGTCCTGCTTTGGCGCGTTTTGGTGTTAGTGCGCCGCTAAAACGCAGTTCGTCGAGGCCATCATCTGCCAGCCGCGCTTTAGTGTAATC                                 <  SRR4302537.1260700/94‑1 (MQ=60)
                                                          CACGGCGTCCTGCTTTGGCGCGTTTTGGTGTTAGTGCGCCGCTAAAACGCAGTTCGTCGAGGCCATCATCTGCCAGCCGCGCTTTAGTGTAATC                                 >  SRR4302537.2925861/1‑94 (MQ=60)
                                                            CGGCGTCCTGCTTTGGCGCGTTTTGGTGTTAGTGCGCCGCTAAAACGCAGTTCGTCGAGGCCATCATCTGCCAGCCGCGCTTTAGTGTAATCGG                               <  SRR4302537.3362985/94‑1 (MQ=60)
                                                             GGCGTCCTGCTTTGGCGCGTTTTGGTGTTAGTGCGCCGCTAAAACGCAGTTCGTCGAGGCCATCATCTGCCAGCCGCGCTTTAGTGTAATCGGA                              >  SRR4302537.2290320/1‑94 (MQ=60)
                                                              GCGTCCTGCTTTGGCGCGTTTTGGTGTTAGTGCGCCGCTAAAACGCAGTTCGTCGAGGCCATCATCTGCCAGCCGCGCTTTAGTGTAATCGGAA                             >  SRR4302537.4776001/1‑94 (MQ=60)
                                                              GCGTCCTGCTTTGGCGCGTTTTGGTGTTAGTGCGCCGCTAAAACGCAGTTCGTCGAGGCCATCATCTGCCAGCCGCGCTTTAGTGTAATCGGAA                             <  SRR4302537.5346019/94‑1 (MQ=60)
                                                               CGTCCTGCTTTGGCGCGTTTTGGTGTTAGTGCGCCGCTAAAACGCAGTTCGTCGAGGCCATCATCTGCCAGCCGCGCTTTAGTGTAATCGGAAC                            >  SRR4302537.5454981/1‑94 (MQ=60)
                                                                GTCCTGCTTTGGCGCGTTTTGGTGTTAGTGCGCCGCTAAAACGCAGTTCGTCGAGGCCATCATCTGCCAGCCGCGCTTTAGTGTAATCGGAACT                           >  SRR4302537.5818813/1‑94 (MQ=60)
                                                                GTCCTGCTTTGGCGCGTTTTGGTGTTAGTGCGCCGCTAAAACGCAGTTCGTCGAGGCCATCATCTGCCAGCCGCGCTTTAGTGTAATCGGAACT                           >  SRR4302537.4302850/1‑94 (MQ=60)
                                                                GTCCTGCTTTGGCGCGTTTTGGTGTTAGTGCGCCGCTAAAACGCAGTTCGTCGAGGCCATCATCTGCCAGCCGCGCTTTAGTGTAATCGGAACT                           <  SRR4302537.4450677/94‑1 (MQ=60)
                                                                 TCCTGCTTTGGCGCGTTTTGGTGTTAGTGCGCCGCTAAAACGCAGTTCGTCGAGGCCATCATCTGCCAGCCGCGCTTTAGTGTAATCGGAACTG                          <  SRR4302537.2825603/94‑1 (MQ=60)
                                                                  CCTGCTTTGGCGCGTTTTGGTGTTAGTGCGCCGCTAAAACGCAGTTCGTCGAGGCCATCATCTGCCAGCCGCGCTTTAGTGTAATCGGAACTGC                         <  SRR4302537.2538328/94‑1 (MQ=60)
                                                                  CCTGCTTTGGCGCGTTTTGGTGTTAGTGCGCCGCTAAAACGCAGTTCGTCGAGGCCATCATCTGCCAGCCGCGCTTTAGTGTAATCGGAACTGC                         >  SRR4302537.5756865/1‑94 (MQ=60)
                                                                   CTGCTTTGGCGCGTTTTGGTGTTAGTGCGCCGCTAAAACGCAGTTCGTCGAGGCCATCATCTGCCAGCCGCGCTTTAGTGTAATCGGAACTGCG                        >  SRR4302537.4499575/1‑94 (MQ=60)
                                                                   CTGCTTTGGCGCGTTTTGGTGTTAGTGCGCCGCTAAAACGCAGTTCGTCGAGGCCATCATCTGCCAGCCGCGCTTTAGTGTAATCGGAACTGCG                        <  SRR4302537.5790237/94‑1 (MQ=60)
                                                                      CTTTGGCGCGTTTTGGTGTTAGTGCGCCGCTAAAACGCAGTTCGTCGAGGCCATCATCTGCCAGCCGCGCTTTAGTGTAATCGGAACTGCGCAC                     <  SRR4302537.6254151/94‑1 (MQ=60)
                                                                      CTTTGGCGCGTTTTGGTGTTAGTGCGCCGCTAAAACGCAGTTCGTCGAGGCCATCATCTGCCAGCCGCGCTTTAGTGTAATCGGAACTGCGCAC                     <  SRR4302537.858842/94‑1 (MQ=60)
                                                                      CTTTGGCGCGTTTTGGTGTTAGTGCGCCGCTAAAACGCAGTTCGTCGAGGCCATCATCTGCCAGCCGCGCTTTAGTGTAATCGGAACTGCGCAC                     <  SRR4302537.647460/94‑1 (MQ=60)
                                                                        TTGGCGCGTTTTGGTGTTAGTGCGCCGCTAAAACGCAGTTCGTCGAGGCCATCATCTGCCAGCCGCGCTTTAGTGTAATCGGAACTGCGCACGG                   <  SRR4302537.2793567/94‑1 (MQ=60)
                                                                        TTGGCGCGTTTTGGTGTTAGTGCGCCGCTAAAACGCAGTTCGTCGAGGCCATCATCTGCCAGCCGCGCTTTAGTGTAATCGGAACTGCGCACGG                   <  SRR4302537.1975156/94‑1 (MQ=60)
                                                                         TGGCGCGTTTTGGTGTTAGTGCGCCGCTAAAACGCAGTTCGTCGAGGCCATCATCTGCCAGCCGCGCTTTAGTGTAATCGGAACTGCGCACGGT                  <  SRR4302537.1368274/94‑1 (MQ=60)
                                                                         TGGCGCGTTTTGGTGTTAGTGCGCCGCTAAAACGCAGTTCGTCGAGGCCATCATCTGCCAGCCGCGCTTTAGTGTAATCGGAACTGCGCACGGT                  >  SRR4302537.905487/1‑94 (MQ=60)
                                                                              CGTTTTGGTGTTAGTGCGCCGCTAAAACGCAGTTCGTCGAGGCCATCATCTGCCAGCCGCGCTTTAGTGTAATCGGAACTGCGCACGGTAAGTT             <  SRR4302537.6316550/94‑1 (MQ=60)
                                                                               GTTTTGGTGTTAGTGCGCCGCTAAAACGCAGTTCGTCGAGGCCATCATCTGCCAGCCGCGCTTTAGTGTAATCGGAACTGCGCACGGTAAGTTC            <  SRR4302537.1053306/94‑1 (MQ=60)
                                                                               GTTTTGGTGTTAGTGCGCCGCTAAAACGCAGTTCGTCGAGGCCATCATCTGCCAGCCGCGCTTTAGTGTAATCGGAACTGCGCACGGTAAGTTC            >  SRR4302537.3374120/1‑94 (MQ=60)
                                                                                TTTTGGTGTTAGTGCGCCGCTAAAACGCAGTTCGTCGAGGCCATCATCTGCCAGCCGCGCTTTAGTGTAATCGGAACTGCGCACGGTAAGTTCT           <  SRR4302537.416965/94‑1 (MQ=60)
                                                                                 TTTGGTGTTAGTGCGCCGCTAAAACGCAGTTCGTCGAGGCCATCATCTGCTAGCCGCGCTTTAGTGTAATCGGAACTGCGCACGGTAAGTTCTT          <  SRR4302537.3480654/94‑1 (MQ=60)
                                                                                 TTTGGTGTTAGTGCGCCGCTAAAACGCAGTTCGTCGAGGCCATCATCTGCCAGCCGCGCTTTAGTGTAATCGGAACTGCGCACGGTAAGTTCTT          <  SRR4302537.831001/94‑1 (MQ=60)
                                                                                 TTTGGTGTTAGTGCGCCGCTAAAACGCAGTTCGTCGAGGCCATCATCTGCCAGCCGCGCTTTAGTGTAATCGGAACTGCGCACGGTAAGTTCTT          <  SRR4302537.3134601/94‑1 (MQ=60)
                                                                                 TTTGGTGTTAGTGCGCCGCTAAAACGCAGTTCGTCGAGGCCATCATCTGCCAGCCGCGCTTTAGTGTAATCGGAACTGCGCACGGTAAGTTATT          <  SRR4302537.2492927/94‑1 (MQ=60)
                                                                                  TTGGTGTTAGTGCGCCGCTAAAACGCAGTTCGTCGAGGCCATCATCTGCCAGCCGCGCTTTAGTGTAATCGGAACTGCGCACGGTAAGTTCTTC         <  SRR4302537.5228279/94‑1 (MQ=60)
                                                                                   TGGTGTTAGTGCGCCGCTAAAACGCAGTTCGTCGAGGCCATCATCTGCCAGCCGCGCTTTAGTGTAATCGGAACTGCGCACGGTAAGTTCTTCG        >  SRR4302537.5753989/1‑94 (MQ=60)
                                                                                   TGGTGTTAGTGCGCCGCTAAAACGCAGTTCGTCGAGGCCATCATCTGCCAGCCGCGCTTTAGTGTAATCGGAACTGCGCACGGTAAGTTCTTCG        <  SRR4302537.5710442/94‑1 (MQ=60)
                                                                                   TGGTGTTAGTGCGCCGCTAAAACGCAGTTCGTCGAGGCCATCATCTGCCAGCCGCGCTTTAGTGTAATCGGAACTGCGCACGGTAAGTTCTTCG        <  SRR4302537.6079138/94‑1 (MQ=60)
                                                                                   TGGTGTTAGTGCGCCGCTAAAACGCAGTTCGTCGAGGCCATCATCTGCCAGCCGCGCTTTAGTGTAATCGGAACTGCGCACGGTAAGTTCTTCG        <  SRR4302537.1163522/94‑1 (MQ=60)
                                                                                   TGGTGTTAGTGCGCCGCTAAAACGCAGTTCGTCGAGGCCATCAGCTGCCAGCCGCGCTTTAGTGTAATCGGAACTGCGCACGGTAAGTTCTTCG        >  SRR4302537.3487710/1‑94 (MQ=60)
                                                                                   TGGTGTTAGTGCGCCGCTAAAACGCAGTTCGTCGAGCCCATCATCTGCCAGCCGCGCTTTAGTGTAATCGGAACTGCGCACGGTAAGTTCTTCG        <  SRR4302537.1961313/94‑1 (MQ=60)
                                                                                    GGTGTTAGTGCGCCGCTAAAACGCAGTTCGTCGAGGCCATCATCTGCCAGCCGCGCTTTAGTGTAATCGGAACTGCGCACGGTAAGTTCTTCGG       >  SRR4302537.345024/1‑94 (MQ=60)
                                                                                    GGTGTTAGTGCGCCGCTAAAACGCAGTTCGTCGAGGCCATCATCTGCCAGCCGCGCTTTAGTGTAATCGGAACTGCGCACGGTAAGTTCTTCGG       <  SRR4302537.941440/94‑1 (MQ=60)
                                                                                    GGTGTTAGTGCGCCGCTAAAACGCAGTTCGTCGAGGCCATCATCTGCCAGCCGCGCTTTAGTGTAATCGGAACTGCGCACGGTAAGTTCTTCGG       >  SRR4302537.3407750/1‑94 (MQ=60)
                                                                                    GGTGTTAGTGCGCCGCTAAAACGCAGTTCGTCGAGGCCATCATCTGCCAGCCGCGCTTTAGTGTAATCGGAACTGCGCACGGTAAGTTCTTCGG       >  SRR4302537.591033/1‑94 (MQ=60)
                                                                                    GGTGTTAGTGCGCCGCTAAAACGCAGTTCGTCGAGGCCATCATCTGCCAGCCGCGCTTTAGTGTAATCGGAACTGCGCACGGTAAGTTCTTCGG       >  SRR4302537.4931043/1‑94 (MQ=60)
                                                                                      TGTTAGTGCGCCGCTAAAACGCAGTTCGTCGAGGCCATCATCTGCCAGCCGCGCTTTAGTGTAATCGGAACTGCGCACGGTAAGTTCTTCGGTA     <  SRR4302537.5915272/94‑1 (MQ=60)
                                                                                      TGTTAGTGCGCCGCTAAAACGCAGTTCGTCGAGGCCATCATCTGCCAGCCGCGCTTTAGTGTAATCGGAACTGCGCACGGGAAGTTCTTCGGTA     >  SRR4302537.4602010/1‑94 (MQ=60)
                                                                                       GTTAGTGCGCCGCTAAAACGCAGTTCGTCGAGGCCATCATCTGCCAGCCGCGCTTTAGTGTAATCGGAACTGCGCACGGTAAGTTCTTCGGTAT    >  SRR4302537.2066282/1‑94 (MQ=60)
                                                                                        TTAGTGCGCCGCTAAAACGCAGTTCGTCGAGGCCATCATCTGCCAGCCGCGCTTTAGTGTAATCGGAACTGCGCACGGTAAGTTCTTCGGTATC   <  SRR4302537.5163622/94‑1 (MQ=60)
                                                                                         TAGTGCGCGGCTAAAACGCAGTTCGTCGAGGCCATCATCTGCCAGCCGCGCTTTAGTGTAATCGGAACTGCGCACGGTAAGTTCTTCGGTATCG  <  SRR4302537.6495298/94‑1 (MQ=60)
                                                                                         TAGTGCGCCGCTAAAACGCAGTTCGTCGAGGCCATCATCTGCCAGCCGCGCTTTAGTGTAATCGGAACTGCGCACGGTAAGTTCTTCGGTATCG  >  SRR4302537.2217586/1‑94 (MQ=60)
                                                                                             |                                                                                         
GCGATGAATTCCATTTCCGGCGTGATGATGCCCTGGCGGGCGTAGTGCAGTTGGGTGACACGGCGTCCTGCTTTGGCGCGTTTTGGTGTTAGTACGCCGCTAAAACGCAGTTCGTCGAGGCCATCATCTGCCAGCCGCGCTTTAGTGTAATCGGAACTGCGCACGGTAAGTTCTTCGGTATCG  >  NC_000913/4193181‑4193363

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑