Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 882 254.4 12957588 98.0% 12698436 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,201,880 A→G Q328R (CAG→CGG)  hydG → HydG

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,201,8800AG99.5% 670.8 / NA 202Q328R (CAG→CGG) hydGHydG
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base G (79/121);  total (79/123)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 227 total aligned reads displayed.

TCTACTATCGCCTGAATGTGGTGGCGATTGAAGTACCATCGCTGCGGCAACGGCGGGAAGATATCCCTCTGCTGGCTGGCCATTTTCTGCAGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTGGCCGGGAAAT  >  NC_000913/4201790‑4201968
                                                                                          |                                                                                        
tctACTATCGCCTGAATGTGGTGGCGATTGAAGTACCATCGCTGCGGCAACGGCGGGAAGATATCCCTCTGCTGGCTGGCCATTTTCTGCGgc                                                                                        <  1:5614013/93‑1 (MQ=255)
  tactaTCGCCTGAATGTGGTGGCGGTTGAAGTACCATCGCTGCGGCAACGGCGGGAAGAAATCGCCCTTCTGGCTGGGCCTTTTCTTCGgcgc                                                                                      >  2:2417319/1‑93 (MQ=255)
  tactaTCGCCTGAATGTGGTGGCGATTGAAGTACCATCGCTGCGGCAACGGCGGGAAGATATCCCTCTGCTGGCTGGCCATTTTCTGCGgcgc                                                                                      >  1:2917280/1‑93 (MQ=255)
  tactaTCGCCTGAATGTGGTGGCGATTGAAGTACCATCGCTGCGGCAACGGCGGGAAGATATCCCTCTGCTGGCTGGCCATTTTCTGCGgcgc                                                                                      >  1:4339393/1‑93 (MQ=255)
    ctaTCGCCTGAATGTGGTGGCGATTGAAGTACCATCGCTGCGGCAACGGCGGGAAGATATCCCTCTGCTGGCTGGCCATTTTCTGCGGCGCtt                                                                                    <  2:6409950/93‑1 (MQ=255)
      aTCGCCTGAATGTGGTGGCGATTGAAGTACCATCGCTGCGGCAACGGCGGGAAGATATCCCTCTGCTGGCTGGCCATTTTCTGCGGCGCTTTg                                                                                  >  2:2036372/1‑93 (MQ=255)
      aTCGCCTGAATGTGGTGGCGATTGAAGTACCATCGCTGCGGCAACGGCGGGAAGATATCCCTCTGCTGGCTGGCCATTTTCTGCGGCGCTTTg                                                                                  >  1:1761560/1‑93 (MQ=255)
      aTCGCCTGAATGTGGTGGCGATTGAAGTACCATCGCTGCGGCAACGGCGGGAAGATATCCCTCTGCTGGCTGGCCATTTTCTGCGGCGCTTTg                                                                                  <  1:3401414/93‑1 (MQ=255)
        cGCCTGAATGTGGTGGCGATTGAAGTACCATCGCTGCGGCAACGGCGGGAAGATATCCCTCTGCTGGCTGGCCATTTTCTGCGGCGCTTTGcc                                                                                <  1:4994058/93‑1 (MQ=255)
         gCCTGAATGTGGTGGCGATTGAAGTACCATCGCTGCGGCAACGGCGGGAAGATATCCCTCTGCTGGCTGGCCATTTTCTGCGGCGCTTTGCCg                                                                               >  2:654272/1‑93 (MQ=255)
          ccTGAATGTGGTGGCGATTGAAGTACCATCGCTGCGGCAACGGCGGGAAGATATCCCTCTGCTGGCTGGCCATTTTCTGCGGCGCTTTGCCGa                                                                              <  1:1211802/93‑1 (MQ=255)
           cTGAATGTGGTGGCGATTGAAGTACCATCGCTGCGGCAACGGCGGGAAGATATCCCTCTGCTGGCTGGCCATTTTCTGCGGCGCTTTGCCGAg                                                                             >  1:5689857/1‑93 (MQ=255)
           cTGAATGTGGTGGCGATTGAAGTACCATCGCTGCGGCAACGGCGGGAAGATATCCCTCTGCTGGCTGGCCATTTTCTGCGGCGCTTTGCCGAg                                                                             <  1:5079846/93‑1 (MQ=255)
            tGAATGTGGTGGCGATTGAAGTACCATCGCTGCGGCAACGGCGGGGAGATATCCCTCTGCTGGCTGGCCATTTTCTGCGGCGCTTTGCCCAGc                                                                            >  2:1701125/1‑93 (MQ=255)
            tGAATGTGGTGGCGATTGAAGTACCATCGCTGCGGCAACGGCGGGAAGATATCCCTCTGCTGGCTGGCCGTTTTCTGCGGCGCGTTGCCGAGc                                                                            >  2:5060252/1‑93 (MQ=255)
                tgtgGTGGCGATTGAAGTACCATCGCTGCGGCAACGGCGGGAAGATATCCCTCTGCTGGCTGGCCATTTTCTGCGGCGCTTTTCCGAGCGTaa                                                                        >  1:235978/1‑93 (MQ=255)
                     tggCGATTGAAGTACCATCGCTGCGGCAACGGCGGGAAGATATCCCTCTGCTGGCTGGCCATTTTCTGCGGCGCTTTGCCGAGCGTAATCGCa                                                                   <  2:166138/93‑1 (MQ=255)
                         gATTGAAGTACCATCGCTGCGGCAACGGCGGGAAGATATCCCTCTGCTGGCTGGCCATTTTCTGCGGCGCTTTGCCGAGCGTAATCGCAAggg                                                               >  2:423685/1‑92 (MQ=255)
                         gATTGAAGTACCATCGCTGCGGCAACGGCGGGAAGATATCCCTCTGCTGGCTGGCCATTTTCTGCGGCGCTTTGCCGAGCGTAATCGCAAGGc                                                               >  2:1551594/1‑93 (MQ=255)
                          aTTGAAGTACCATCGCTGCGGCAACGGCGGGAAGATATCCCTCTGCTGGCTGGCCATTTTCTGCGGCGCTTTGCCGAGCGTAATCGCAAGGCg                                                              <  2:629607/93‑1 (MQ=255)
                          aTTGAAGTACCATCGCTGCGGCAACGGCGGGAAGATATCCCTCTGCTGGCTGGCCATTTTCTGCGGCGCTTTGCCGAGCGTAATCGCAAGGCg                                                              <  1:980890/93‑1 (MQ=255)
                          aTTGAAGTACCATCGCTGCGGCAACGGCGGGAAGATATCCCTCTGCTGGCTGGCCATTTTCTGCGGCGCTTTGCCGAGCGGAATTGCAAcgcg                                                              >  2:3722366/1‑93 (MQ=255)
                           ttGAAGTACCATCGCTGCGGCAACGGCTGGAAGATATCCCTCTGCTGGCTGGCCATTTTCTGCGGCGCTTTGCCGAGCGTAATCGCAAGGCgg                                                             >  1:2094699/1‑93 (MQ=255)
                           ttGAAGTACCATCGCTGCGGCAACGGCGGGAAGATATCCCTCTGCTGGCTGGGCATTTTCTGCGGCGATTTGCCGGGCGTAATCGCAAGGccg                                                             >  1:784718/1‑91 (MQ=255)
                           ttGAAGTACCATCGCTGCGGCAACGGCGGGAAGATATCCCTCTGCTGGCTGGCCATTTTCTGCGGCGCTTTGCCGAGCGTAATCGCAAGGCgg                                                             >  1:2529838/1‑93 (MQ=255)
                           ttGAAGTACCATCGCTGCGGCAACGGCGGGAAGATATCCCTCTGCTGGCTGGCCATTTTCTGCGGCGCTTTGCCGAGCGTAATCGCAAGGCgg                                                             >  1:6317068/1‑93 (MQ=255)
                            tGAAGTACCATCGCTGCGGCAACGGCGGGAAGATATCCCTCTGCTGGCTCGCGATTTTCTGCGGCGCTTTGCCGAGCGTAATCGCCAGGCGGt                                                            <  2:1912742/93‑1 (MQ=255)
                             gAAGTACCATCGCTGCGGCAACGGCGGGGAGATATCCCTCTGCTGGCTGGCCATTTTCTGCGGCGCTTTTCCGAGCGGAATCGCAAAGCggga                                                           >  1:2287082/1‑91 (MQ=255)
                             gAAGTACCATCGCTGCGGCAACGGCGGGAAGATATCCCTCTGCTGGCTGGCCATTTTCTGCGGCGCTTTGCCGAGCGTAATCGCAAAGCGGTa                                                           >  1:5739942/1‑93 (MQ=255)
                               aGTACCATCGCTGCGGCAACGGCGGGAAGATATCCCTCTGCTGGCTGGCCATTTTCTGCGGCGCTTTGCCGAGCGTAATCGCAAGGCGGTaaa                                                         <  2:3615281/93‑1 (MQ=255)
                               aGTACCATCGCTGCGGCAACGGCGGGAAGATATCCCTCTGCTGGCTGGCCATTTTCTGCGGCGCTTTGCCGAGCGTAATCGCAAGGCGGTaaa                                                         >  2:5961688/1‑93 (MQ=255)
                                 tACCATCGCTGCGGCAACGGCGGGAAGATATCCCTCTGCTGGCTGGCCATTTTCTGCGGCGCTTTGCCGAGCGTAATCGCAAAGCGGGAAAAg                                                       >  1:2867103/1‑93 (MQ=255)
                                   ccATCGCTGCGGCAACGGCGGGAAGATATCCCTCTGCTGGCTGGCCATTTTCTGCGGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGt                                                     >  1:4126972/1‑93 (MQ=255)
                                   ccATCGCTGCGGCAACGGCGGGAAGATATCCCTCTGCTGGCTGGCCATTTTCTGCGGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGt                                                     <  1:5515606/93‑1 (MQ=255)
                                   ccATCGCTGCGGCAACGGCGGGAAGATATCCCTCTGCTGGCTGGCCATTTTCTGCGGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGt                                                     <  2:6042525/93‑1 (MQ=255)
                                    cATCGCTGCGGCAACGGCGGGAAGATATCCCTCTGCTGGCTGGCCATTTTCTGCGGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGtt                                                    <  1:68597/93‑1 (MQ=255)
                                      tCGCTGCGGCAACGGCGGGAAGATATCCCTCTGCTGGCTGGCCATTTTCTGCGGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGtttt                                                  >  2:1113918/1‑93 (MQ=255)
                                      tCGCTGCGGCAACGGCGGGAAGATATCCCTCTGCTGGCTGGCCATTTTCTGCGGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGtttt                                                  >  1:4487963/1‑93 (MQ=255)
                                       cGCTGCGGCAACGGCGGGAAGATATCCCTCTGCTGGCTGGCCATTTTCTGCGGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTa                                                 <  1:480886/93‑1 (MQ=255)
                                        gCTGCGGCAACGGCGGGAAGATATCCCTCTGCTGGCTGGCCATTTTCTGCGGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTAc                                                <  1:22458/93‑1 (MQ=255)
                                        gCTGCGGCAACGGCGGGAAGATATCCCTCTGCTGGCTGGCCATTTTCTGCGGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTAc                                                <  1:5304233/93‑1 (MQ=255)
                                          tGCGGCAACGGCGGGAAGATATCCCTCTGCTGGCTGGCCATTTTCTGCGGCGCTTTGCCGAGCGTAATCGCCAGGCGGTAAAAGGGTTTACGc                                              >  2:250614/1‑93 (MQ=255)
                                           gCGGCAACGGCGGGAAGATATCCCTCTGCTGGCTGGCCATTTTCTGCGGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGcc                                             <  1:149085/93‑1 (MQ=255)
                                           gCGGCAACGGCGGGAAGATATCCCTCTGCTGGCTGGCCATTTTCTGCGGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGcc                                             >  1:4027219/1‑93 (MQ=255)
                                           gCGGCAACGGCGGGAAGATATCCCTCTGCTGGCTGGCCATTTTCTGCGGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGcc                                             <  2:2752973/93‑1 (MQ=255)
                                            cGGCAACGGCGGGAAGATATCCCTCTGCTGGCTGGCCATTTTCTGCGGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGccc                                            <  2:5128174/93‑1 (MQ=255)
                                             ggCAACGGCGGGAAGATATCCCTCTGCTGGCTGGCCATTTTCTGCGGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGcccc                                           <  1:6051595/93‑1 (MQ=255)
                                              gCAACGGCGGGAAGATATCCCTCTGCTGGCTGGCCATTTTCTGCGGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCa                                          <  2:5594678/93‑1 (MQ=255)
                                              gCAACGGCGGGAAGATATCCCTCTGCTGGCTGGCCATTTTCTGCGGCGCTTTGCCGAGCGTAATCGCAACGCGGTAAAAAGTTTTACGCCCCa                                          >  2:705815/1‑93 (MQ=255)
                                                aaCGGCGGGAAGATATCCCTCTGCTGGCTGGCCATTTTCTGCGGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCAgg                                        <  2:3936153/93‑1 (MQ=255)
                                                aaCGGCGGGAAGATATCCCTCTGCTGGCTGGCCATTTTCTGCGGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCAgg                                        >  2:6253042/1‑93 (MQ=255)
                                                 aCGGCGGGAAGATATTCCTCTGCTGGCTGGCCATTTTCTGCGGCGCTTTGCCCCGCGTAATCGCAAAGCGGGAAAAGGTTTTACGCCCCAAGc                                       >  2:3378683/1‑93 (MQ=255)
                                                  cggcggGAAGATATCCCTCTGCTGGCTGGCCATTTTCTGCGGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCg                                      <  2:4863247/93‑1 (MQ=255)
                                                   ggcggGAAGATATCCCTCTGCTGGCTGGCCATTTTCTGCGGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGa                                     <  1:6025058/93‑1 (MQ=255)
                                                    gcggGAAGATATCCCTCTGCTGGCTGGCCATTTTCTGCGGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGAt                                    <  2:2899846/93‑1 (MQ=255)
                                                     cggGAAGATATCCCTCTGCTGGCTGGCCATTTTCTGCGGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATg                                   >  1:1334419/1‑93 (MQ=255)
                                                      gggAAGATATCCCTCTGCTGGCTGGCCATTTTCTGCGGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATgg                                  <  1:5840440/93‑1 (MQ=255)
                                                      gggAAGATATCCCTCTGCTGGCTGGCCATTTTCTGCGGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATgg                                  >  2:2304989/1‑93 (MQ=255)
                                                       ggAAGATATCCCTCTGCTGGCTGGCCATTTTCTGCGGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGa                                 <  1:4898491/93‑1 (MQ=255)
                                                        gAATATATCCCTCTGCTGGCTGTCCATTTTCTGCAGCGCTTTGCCTAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGAt                                <  2:1985216/93‑1 (MQ=255)
                                                          aGATATCCCTCTGCTGGCTGGCCATTTTCTGCGGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCt                              >  1:3117429/1‑93 (MQ=255)
                                                            atatCCCTCTGCTGGCTGGCCATTTTCTGCGGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGt                            <  1:272710/93‑1 (MQ=255)
                                                             tatCCCTCTGCTGGCTGGCCATTTTCTGCGGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCGCAGGCGATGGATCTGtt                           <  2:4177936/93‑1 (MQ=255)
                                                             tatCCCTCTGCTGGCTGGCCATTTTCTGCGGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGtt                           <  2:2710834/93‑1 (MQ=255)
                                                              atCCCTCTGCTGGCTGGCCATTTTCTGCGGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTg                          >  1:915572/1‑93 (MQ=255)
                                                               tCCCTCTGCTGGCTGGCCATTTTCTGCGGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGa                         <  2:3018931/93‑1 (MQ=255)
                                                                 ccTCTGCTGGCTGGCCATTTTCTGCGGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGAtt                       >  2:388711/1‑93 (MQ=255)
                                                                 ccTCTGCTGGCTGGCCATTTTCTGCGGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGAtt                       <  2:2262478/93‑1 (MQ=255)
                                                                 ccTCTGCTGGCTGGCCATTTTCTGCGGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGAtt                       >  1:2902248/1‑93 (MQ=255)
                                                                 ccTCTGCTGGCTGGCCATTTTCTGCGGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGAtt                       <  2:1244020/93‑1 (MQ=255)
                                                                 ccTCTGCTGGCTGGCCATTTTCTGCGGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGAtt                       >  2:5691509/1‑93 (MQ=255)
                                                                  ctctGCTGGCTGGCCATTTTCTGCGGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTc                      <  2:404254/93‑1 (MQ=255)
                                                                   tctGCTGGCTGGCCATTTTCTGCGGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCa                     <  1:1549348/93‑1 (MQ=255)
                                                                    ctgctgGCTGGCCATTTTCTGCGGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCAt                    <  2:4717892/93‑1 (MQ=255)
                                                                    ctgctgGCTGGCCATTTTCTGCGGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCAt                    >  1:2053180/1‑93 (MQ=255)
                                                                     tgctgGCTGGCCATTTTCTGCGGCGCTTTGCCGAGCGTAATCGCAATGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCAtt                   >  2:5245599/1‑93 (MQ=255)
                                                                     tgctgGCTGGCCATTTTCTGCGGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCAtt                   >  1:5459297/1‑93 (MQ=255)
                                                                     tgctgGCTGGCCATTTTCTGCGGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCAtt                   >  1:1001574/1‑93 (MQ=255)
                                                                     tgctgGCTGGCCATTTTCTGCGGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCAtt                   <  2:4410437/93‑1 (MQ=255)
                                                                      gctggctgGCCATTTTCTGCGGCGCTTTGCCGAGCGTAATCGCAAGTCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTa                  <  2:5202967/93‑1 (MQ=255)
                                                                       ctggctggTCATTTTCTGCGGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTAc                 <  2:4558000/93‑1 (MQ=255)
                                                                        tggctggcCATTTTCTGCGGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACg                <  2:2380829/93‑1 (MQ=255)
                                                                         ggctggcCATTTTCTGCGGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGa               <  1:1133837/93‑1 (MQ=255)
                                                                         ggctggcCATTTTCTGCGGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGa               <  1:3558157/93‑1 (MQ=255)
                                                                         ggctggcCATTTTCTGCGGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGa               >  2:1497383/1‑93 (MQ=255)
                                                                          gctggcCATTTTCTGCGGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGAc              >  2:2158329/1‑93 (MQ=255)
                                                                          gctggcCATTTTCTGCGGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGAc              <  2:5526753/93‑1 (MQ=255)
                                                                            tggcCATTTTCTGCGGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTg            >  2:795530/1‑93 (MQ=255)
                                                                            tggcCATTTTCTGCGGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTg            >  2:2819345/1‑93 (MQ=255)
                                                                             ggcCATTTTCTGCGGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTgg           >  1:6106454/1‑93 (MQ=255)
                                                                             ggcCATTTTCTGCGGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTgg           >  1:4424701/1‑93 (MQ=255)
                                                                              gcCATTTTCTGCGGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTGGc          <  1:5156474/93‑1 (MQ=255)
                                                                              gcCATTTTCTGCGGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTGGc          >  2:3306607/1‑93 (MQ=255)
                                                                                cATTTTCTGGGGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTGGCCg        <  2:4775842/93‑1 (MQ=255)
                                                                                cATTTTCTGCGGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTGGCCg        <  1:3308767/93‑1 (MQ=255)
                                                                                 aTTTTCTGCGGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTGGCCgg       >  1:1659368/1‑93 (MQ=255)
                                                                                  ttttCTGCGGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTGGCCggg      >  2:2593958/1‑93 (MQ=255)
                                                                                   tttCTGCGGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTGGCCGGGa     >  1:4552650/1‑93 (MQ=255)
                                                                                     tCTGCGGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTGGCCGGGaaa   <  1:3818190/93‑1 (MQ=255)
                                                                                      cTGCGGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTGGCCGGGAAat  >  2:2895471/1‑93 (MQ=255)
                                                                                          |                                                                                        
TCTACTATCGCCTGAATGTGGTGGCGATTGAAGTACCATCGCTGCGGCAACGGCGGGAAGATATCCCTCTGCTGGCTGGCCATTTTCTGCAGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTGGCCGGGAAAT  >  NC_000913/4201790‑4201968

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ATCTCTACTATCGCCTGAATGTGGTGGCGATTGAAGTACCATCGCTGCGGCAACGGCGGGAAGATATCCCTCTGCTGGCTGGCCATTTTCTGCAGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTGGCCGGGAAATATTCG  >  NC_000913/4201787‑4201973
                                                                                             |                                                                                             
GTCTCTACTATCGCCTGAATGTGGTGGCGATTGAAGTACCATCGCTGCGGCAACGGCGGGAAGATATCCCTCTGCTGGCTGGCCATTTTCTGCG                                                                                               <  SRR4302537.5240347/94‑1 (MQ=60)
ATCTCTACTATCGCCTGAATGTGGTGGCGATTGAAGTACCATCGCTGCGGCAACGGCGGGAAGATATCCCTCTGCTGGCTGGCCATTTTCTGCG                                                                                               <  SRR4302537.4146125/94‑1 (MQ=60)
   TCTACTATCGCCTGAATGTGGTGGCGATTGAAGTACCATCGCTGCGGCAACGGCGGGAAGATATCCCTCTGCTGGCTGGCCATTTTCTGCGGCG                                                                                            <  SRR4302537.5652396/94‑1 (MQ=60)
   TCTACTATCGCCTGAATGTGGTGGCGATTGAAGTACCATCGCTGCGGCAACGGCGGGAAGATATCCCTCTGCTGGCTGGCCATTTTCTGCGGCG                                                                                            >  SRR4302537.2323034/1‑94 (MQ=60)
    CTACTATCGCCTGAATGTGGTGGCGATTGAAGTACCATCGCTGCGGCAACGGCGGGAAGATATCCCTCTGCTGGCTGGCCATTTTCTGCGGCGC                                                                                           >  SRR4302537.2937775/1‑94 (MQ=60)
    CTACTATCGCCTGAATGTGGTGGCGATTGAAGTACCATCGCTGCGGCAACGGCGGGAAGATATCCCTCTGCTGGCTGGCCATTTTCTGCGGCGC                                                                                           >  SRR4302537.4369505/1‑94 (MQ=60)
     TACTATCGCCTGAATGTGGTGGCGATTGAAGTACCATCGCTGCGGCAACGGCGGGAAGATATTCCTCTGCTGGCTGGCCATTTTCTGCGGCGCT                                                                                          >  SRR4302537.4804774/1‑94 (MQ=60)
        TATCGCCTGAATGTGGTGGCGATTGAAGTACCATCGCTGCGGCAACGGCGGGAAGATATCCCTCTGCTGGCTGGCCATTTTCTGCGGCGCTTTG                                                                                       >  SRR4302537.1773836/1‑94 (MQ=60)
        TATCGCCTGAATGTGGTGGCGATTGAAGTACCATCGCTGCGGCAACGGCGGGAAGATATCCCTCTGCTGGCTGGCCATTTTCTGCGGCGCTTTG                                                                                       <  SRR4302537.6201586/94‑1 (MQ=60)
        TATCGCCTGAATGTGGTGGCGATTGAAGTACCATCGCTGCGGCAACGGCGGGAAGATATCCCTCTGCTGGCTGGCCATTTTCTGCGGCGCTTTG                                                                                       <  SRR4302537.1448474/94‑1 (MQ=60)
         ATCGCCTGAATGTGGTGGCGATTGAAGTACCATCGCTGCGGCAACGGCGGGAAGATATCCCTCTGCTGGCTGGCCATTTTCTGCGGCGCTTTGC                                                                                      <  SRR4302537.3425507/94‑1 (MQ=60)
           CGCCTGAATGTGGTGGCGATTGAAGTACCATCGCTGCGGCAACGGCGGGAAGATATCCCTCTGCTGGCTGGCCATTTTCTGCGGCGCTTTGCCG                                                                                    <  SRR4302537.5028470/94‑1 (MQ=60)
             CCTGAATGTGGTGGCGATTGAAGTACCATCGCTGCGGCAACGGCGGGAAGATATCCCTCTGCTGGCTGGCCATTTTCTGCGGCGCTTTGCCGAG                                                                                  <  SRR4302537.1220234/94‑1 (MQ=60)
             CCTGAATGTGGTGGCGATTGAAGTACCATCGCTGCGGCAACGGCGGGAAGATATCCCTCTGCTGGCTGGCCATTTTCTGCGGCGCTTTGCCGAG                                                                                  >  SRR4302537.5728774/1‑94 (MQ=60)
             CCTGAATGTGGTGGCGATTGAAGTACCATCGCTGCGGCAACGGCGGGAAGATATCCCTCTGCTGGCTGGCCATTTTCTGCGGCGCTTTGCCGAG                                                                                  <  SRR4302537.699890/94‑1 (MQ=60)
             CCTGAATGTGGTGGCGATTGAAGTACCATCGCTGCGGCAACGGCGGGAAGATATCCCTCTGCTGGCTGGCCATTTTCTGCAGCGCTTTGCCGAG                                                                                  >  SRR4302537.5898043/1‑94 (MQ=60)
              CTGAATGTGGTGGCGATTGAAGTACCATCGCTGCGGCAACGGCGGGAAGATATCCCTCTGCTGGCTGGCCATTTTCTGCGGCGCTTTGCCGAGC                                                                                 <  SRR4302537.5114816/94‑1 (MQ=60)
              CTGAATGTGGTGGCGATTGAAGTACCATCGCTGCGGCAACGGCGGGAAGATATCCCTCTGCTGGCTGGCCATTTTCTGCGGCGCTTTGCCGAGC                                                                                 <  SRR4302537.983459/94‑1 (MQ=60)
              CTGAATGTGGTGGCGATTGAAGTACCATCGCTGCGGCAACGGCGGGAAGATATCCCTCTGCTGGCTGGCCATTTTCTGCGGCGCTTTGCCGAGC                                                                                 >  SRR4302537.1311951/1‑94 (MQ=60)
               TGAATGTGGTGGCGATTGAAGTACCATCGCTGCGGCAACGGCGGGGAGATATACCTCTGCTGGCTGGCCCTTTTCTGCGGCGCTTTGCCGAGCG                                                                                >  SRR4302537.2583327/1‑94 (MQ=60)
                  ATGTGGTGGCGATTGAAGTACCATCGCTGCGGCAACGGCGGGAAGATATCCCTCTGCTGGCTGGCCATTTTCTGCGGCGCTTTTCCGAGCGTAA                                                                             >  SRR4302537.237644/1‑94 (MQ=60)
                   TGTGGTGTCGATTGAAGTACCATCGCTGCGGCAACGGCGGGAAGATATCCCTCTGCTGGCTGGCCATTTTCTGCGGCGCTTTGCCGAGCGTAAT                                                                            <  SRR4302537.2937440/94‑1 (MQ=60)
                    GTGGTGGCGATTGAAGTACCATCGCTGCGGCAACGGCGGGAAGATATCCCTCTGCTGGCTGGCCATTTTCTGCGGCGCTTTGCCGAGCGTAATC                                                                           <  SRR4302537.210743/94‑1 (MQ=60)
                     gtGTGGCGATTGAAGTACCATCGCTGCGGCAACGGCGGGAAGATATCCCTCTGCTGGCTGGCCATTTTCTGCGGCGCTTTGCCGAGCGTAATCG                                                                          <  SRR4302537.5266545/92‑1 (MQ=60)
                     GGGTGGCGATTGAAGTACCATCGCTGCGGCAACGGCGGGAAGATATCCCTCTGCTGGCTGGCCATTTTCTGCGGCGCTTTGCCGAGCGTAATCG                                                                          <  SRR4302537.3882461/94‑1 (MQ=60)
                       GTGGCGATTGAAGTACCATCGCTGCGGCAACGGCGGGAAGATATCCCTCTGCTGGCTGGCCATTTTCTGCGGCGCTTTGCCGAGCGTAATCGCA                                                                        <  SRR4302537.3232656/94‑1 (MQ=60)
                        TGGCGATTGAAGTACCATCGCTGCGGCAACGGCGGGAAGATATCCCTCTGCTGGCTGGCCATTTTCTGCGGCGCTTTGCCGAGCGTAATCGCAA                                                                       <  SRR4302537.5756846/94‑1 (MQ=60)
                         GGNGNNNGNAGNANCATCGNCNNNGCAACGNCGGGAAGANATNCCTCTGCTGGCTGGCCATTTTCTGCGGCNCTTTGCCGAGCGTAATCGCAAG                                                                      <  SRR4302537.1391350/94‑1 (MQ=60)
                         GGCGATTGAAGTACCATCGCTGCGGCAACGGCGGGAAGATATCCCTCTGCTGGCTGGCCATTTTCTGCGGCGCTTTGCCGAGCGTAATCGCAAG                                                                      <  SRR4302537.4956992/94‑1 (MQ=60)
                           CGATTGAAGTACCATCGCTGCGGCAACGGCGGGAAGAAGTCCCTCTCCTGGCTGGCCATTTTCTGCGGCGCTTTGCCGCGCGTAAACGCAAGag                                                                    >  SRR4302537.6448526/1‑92 (MQ=60)
                            GATTGAAGTACCATCGCTGCGGCAACGGCGGGAAGATATCCCTCTGCTGGCTGGCCATTTTCTGCGGCGCTTTGCCGAGCGTAATCGCAAGGCG                                                                   >  SRR4302537.4383951/1‑94 (MQ=60)
                             ATTGAAGTACCATCGCTGCGGCAACGGCTGGAAGATATCCCTCTGCTGGCTGGCCATTTTCTGCGGCGCTTTGCCGAGCGTAATCGCAAGGCGG                                                                  >  SRR4302537.2109471/1‑94 (MQ=60)
                             ATTGAAGTACCATCGCTGCGGCAACGGCGGGAAGATATCCCTCTGCTGGCTGGGCATTTTCTGCGGCGATTTGCCGGGCGTAATCGCAAGGCCG                                                                  >  SRR4302537.790101/1‑94 (MQ=60)
                             ATTGAAGTACCATCGCTGCGGCAACGGCGGGAAGATATCCCTCTGCTGGCTGGCCATTTTCTGCGGCGCTTTGCCGAGCGTAATCGCAAGGCGG                                                                  <  SRR4302537.446538/94‑1 (MQ=60)
                             ATTGAAGTACCATCGCTGCGGCAACGGCGGGAAGATATCCCTCTGCTGGCTGGCCATTTTCTGCGGCGCTTTGCCGAGCGTAATCGCAAGGCGG                                                                  >  SRR4302537.6360298/1‑94 (MQ=60)
                             ATTGAAGTACCATCGCTGCGGCAACGGCGGGAAGATATCCCTCTGCTGGCTGGCCATTTTCTGCGGCGCTTTGCCGAGCGTAATCGCAAGGCGG                                                                  >  SRR4302537.4854863/1‑94 (MQ=60)
                             ATTGAAGTACCATCGCTGCGGCAACGGCGGGAAGATATCCCTCTGCTGGCTGGCCATTTTCTGCGGCGCTTTGCCGAGCGTAATCGCAAGGCGG                                                                  <  SRR4302537.987665/94‑1 (MQ=60)
                             ATTGAAGTACCATCGCTGCGGCAACGGCGGGAAGATATCCCTCTGCTGGCTGGCCATTTTCTGCGGCGCTTTGCCGAGCGTAATCGCAAGGCGG                                                                  <  SRR4302537.6079546/94‑1 (MQ=60)
                             ATTGAAGTACCATCGCTGCGGCAACGGCGGGAAGATATCCCTCTGCTGGCTGGCCATTTTCTGCGGCGCTTTGCCGAGCGTAATCGCAAGGCGG                                                                  >  SRR4302537.2207282/1‑94 (MQ=60)
                             ATTGAAGTACCATCGCTGCGGCAACGGCGGGAAGATATCCCTCTGCTGGCTGGCCATTTTCTGCGGCGCTTTGCCGAGCGTAATCGCAAGGCGG                                                                  >  SRR4302537.2547588/1‑94 (MQ=60)
                               TGAAGTACCATCGCTGCGGCAACGGCGGGGAGATATCCCTCTGCTGGCTGGCCATTTTCTGCGGCGCTTTTCCGAGCGGAATCGCAAAGCGGGA                                                                >  SRR4302537.2303214/1‑94 (MQ=60)
                               TGAAGTACCATCGCTGCGGCAACGGCGGGAAGATATCCCTCTGCTGGCTGGCCATTTTCTGCGGCGCTTTGCCGAGCGTAATCGCAAAGCGGTA                                                                >  SRR4302537.5779245/1‑94 (MQ=60)
                                   GTACCATCGCTGCGGCAACGGCGGGAAGATATCCCTCTGCTGGCTGGCCATTTTCTGCGGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAG                                                            <  SRR4302537.5684794/94‑1 (MQ=60)
                                   GTACCATCGCTGCGGCAACGGCGGGAAGATATCCCTCTGCTGGCTGGCCATTTTCTGCGGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAG                                                            <  SRR4302537.5705659/94‑1 (MQ=60)
                                   GTACCATCGCTGCGGCAACGGCGGGAAGATATCCCTCTGCTGGCTGGCCATTTTCTGCGGCGCTTTGCCGAGCGTAATCGCAAAGCGGGAAAAG                                                            >  SRR4302537.2887223/1‑94 (MQ=60)
                                    TACCATCGCTGCGGCAACGGCGGGAAGATATCCCTCTGCTGGCTGGCCATTTTCTGCGGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGG                                                           <  SRR4302537.6496113/94‑1 (MQ=60)
                                     ACCATCGCTGCGGCAACGGCGGGAAGATATCCCTCTGCTGGCTGGCCATTTTCTGCGGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGT                                                          >  SRR4302537.4155776/1‑94 (MQ=60)
                                      CCATCGCTGCGGCAACGGCGGGAAGATATCCCTCTGCTGGCTGGCCATTTTCTGCGGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTT                                                         <  SRR4302537.5553266/94‑1 (MQ=60)
                                      CCATCGCTGCGGCAACGGCGGGAAGACATCCCTCTGCTGGCTGGCCATTTTCTGCGGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTT                                                         >  SRR4302537.2919771/1‑94 (MQ=60)
                                       CATCGCTGCGGCAACGGCGGGAAGATATCCCTCTGCTGGCTGGCCATTTTCTGCGGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTT                                                        <  SRR4302537.69099/94‑1 (MQ=60)
                                       CATCGCTGCGGCAACGGCGGGAAGATATCCCTCTGCTGGCTGGCCATTTTCTGCGGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGGTT                                                        >  SRR4302537.2360912/1‑94 (MQ=60)
                                        ATCGCTGCGGCAACGGCGGGAAGATATCCCTCTGCTGGCTGGCCATTTTCTGCGGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTT                                                       >  SRR4302537.4519009/1‑94 (MQ=60)
                                          CGCTGCGGCAACGGCGGGAAGATATCCCTCTGCTGGCTGGCCATTTTCTGCGGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTAC                                                     <  SRR4302537.484180/94‑1 (MQ=60)
                                           GCTGCGGCAACGGCGGGAAGATATCCCTCTGCTGGCTGGCCATTTTCTGCGGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACG                                                    <  SRR4302537.3047498/94‑1 (MQ=60)
                                           GCTGCGGCAACGGCGGGAAGATATCCCTCTGCTGGCTGGCCATTTTCTGCGGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACG                                                    <  SRR4302537.5340691/94‑1 (MQ=60)
                                           GCTGCGGCAACGGCGGGAAGATATCCCTCTGCTGGCTGGCCATTTTCTGCGGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACG                                                    <  SRR4302537.22631/94‑1 (MQ=60)
                                            CTGCGGCAACGGCGGGAAGATATCCCTCTGCTGGCTGGCCATTTTCTGCGGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGC                                                   <  SRR4302537.2383016/94‑1 (MQ=60)
                                             TGCGGCAACGGCGGGAAGATATCCCTCTTCTGGCCGGCCATTTTCTGCGGCGCTTTGCCGCGCGGCATCGCAAGGCGGcaagagggtttgccac                                                  >  SRR4302537.1964327/1‑78 (MQ=60)
                                             TGCGGCAACGGCGGGAAGATATCCCTCTGCTGGCTGGCCATTTTCTGCGGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCC                                                  >  SRR4302537.4055412/1‑94 (MQ=60)
                                             TGCGGCAACGGCGGGAAGATATCCCTCTGCTGGCTGGCCATTTTCTGCGGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCC                                                  <  SRR4302537.1158564/94‑1 (MQ=60)
                                             TGCGGCAACGGCGGGAAGATATCCCTCTGCTGGCTGGCCATTTTCTGCGGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCC                                                  <  SRR4302537.3391546/94‑1 (MQ=60)
                                             TGCGGCAACGGCGGGAAGATATCCCTCTGCTGGCTGGCCATTTTCTGCGGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCC                                                  <  SRR4302537.1621573/94‑1 (MQ=60)
                                             TGCGGCAACGGCGGGAAGATATCCCTCTGCTGGCTGGCCATTTTCTGCGGCGCTTTGCCGAGCGTAATCGCAAAGCGGTAAAAGGGTTTACGGC                                                  >  SRR4302537.4850720/1‑94 (MQ=60)
                                              GCGGCAACGGCGGGAAGATATCCCTCTGCTGGCTGGCCATTTTCTGCGGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCC                                                 <  SRR4302537.150153/94‑1 (MQ=60)
                                                GGCAACGGCGGGAAGATATCCCTCTGCTGGCTGGCCATTTTCTGCGGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCA                                               <  SRR4302537.6093163/94‑1 (MQ=60)
                                                   AACGGCGGGAAGATATCCCTCTGCTGGCTGGCCCTTTTCTGCGGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAAGTTTTACGCCCCcggg                                            >  SRR4302537.1053268/1‑90 (MQ=60)
                                                   AACGGCGGGAAGATATCCCTCTGCTGGCTGGCCATTTTCTGCGGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGC                                            <  SRR4302537.4440101/94‑1 (MQ=60)
                                                    ACGGCGGGAAGATATCCCTCTGCTGGCTGGCCATTTTCTGCGGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCG                                           <  SRR4302537.1511824/94‑1 (MQ=60)
                                                      GGCGGGAAGATATCCCTCTGCTGGCTGGCCATTTTCTGCGGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGAT                                         <  SRR4302537.6066458/94‑1 (MQ=60)
                                                      GGCGGGAAGATATCCCTCTGCTGGCTGGCCATTTTCTGCGGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGAT                                         >  SRR4302537.3539513/1‑94 (MQ=60)
                                                       GCGGGAAGATATCCCTCTGCTGGCTGGCCATTTTCTGCGGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATG                                        >  SRR4302537.1343714/1‑94 (MQ=60)
                                                        CGGGAAGATATCCCTCTGCTGGCTGGCCATTTTCTGCGGCGCTTTGCCGAGCGTAATCGCAAAGCGGCAAAAGGTTTTACGCCCCAGGCGATGG                                       >  SRR4302537.3770626/1‑94 (MQ=60)
                                                         GGGAAGATATCCCTCTGCTGGCTGGCCATTTTCTGCGGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGA                                      >  SRR4302537.1717397/1‑94 (MQ=60)
                                                         GGGAAGATATCCCTCTGCTGGCTGGCCATTTTCTGCGGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGA                                      <  SRR4302537.5880487/94‑1 (MQ=60)
                                                         GGGAAGATATCCCTCTGCTGGCTGGCCATTTTCTGCGGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGA                                      >  SRR4302537.5916494/1‑94 (MQ=60)
                                                         GGGAAGATATCCCTCTGCTGGCTGGCCATTTTCTGCGGCGCTTTGACGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGA                                      >  SRR4302537.5803720/1‑94 (MQ=60)
                                                          GGAAGATATCCCTCTGCTGGCTGGCCATTTTCTGCGGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGAT                                     <  SRR4302537.4932305/94‑1 (MQ=60)
                                                           GAAGATATCCCTCTGCTGGCTGGCCATTTTCTGCGGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATC                                    <  SRR4302537.2803471/94‑1 (MQ=60)
                                                            AAGATATCCCTCTGCTGGCTGGCCATTTTCTGCGGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCT                                   >  SRR4302537.3139205/1‑94 (MQ=60)
                                                            AAGATATCCCTCTGCTGGCTGGCCATTTTCTGCGGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCT                                   <  SRR4302537.2282635/94‑1 (MQ=60)
                                                               ATATCCCTCTGCTGGCTGGCCATTTTCTGCGGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTT                                <  SRR4302537.274628/94‑1 (MQ=60)
                                                               ATATCCCTCTGCTGGCTGGCCATTTTCTGCGGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTT                                <  SRR4302537.5545767/94‑1 (MQ=60)
                                                                TATCCCTCTGCTGGCTGGCCATTTTCTGCGGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTG                               >  SRR4302537.921898/1‑94 (MQ=60)
                                                                   CCCTCTGCTGGCTGGCCATTTTCTGCGGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATT                            >  SRR4302537.2922635/1‑94 (MQ=60)
                                                                    CCTCTGCTGGCTGGCCATTTTCTGCGGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTC                           <  SRR4302537.2027756/94‑1 (MQ=60)
                                                                      TCTGCTGGCTGGCCATTTTCTGCGGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCAT                         >  SRR4302537.2067583/1‑94 (MQ=60)
                                                                      TCTGCTGGCTGGCCATTTTCTGCGGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCAT                         <  SRR4302537.1560159/94‑1 (MQ=60)
                                                                      TCTGCTGGCTGGCCATTTTCTGCGGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCAT                         <  SRR4302537.5171210/94‑1 (MQ=60)
                                                                       CTGCTGGCTGGCCATTTTCTGCGGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATT                        >  SRR4302537.1008486/1‑94 (MQ=60)
                                                                       CTGCTGGCTGGCCATTTTCTGCGGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATT                        >  SRR4302537.5496538/1‑94 (MQ=60)
                                                                        TGCTGGCTGGCCATTTTCTGCGGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTA                       <  SRR4302537.5592420/94‑1 (MQ=60)
                                                                         GCTGGCTGGCCATTTTCTGCGGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTAC                      <  SRR4302537.5202707/94‑1 (MQ=60)
                                                                         GCTGGCTGGCCATTTTCTGCGGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTAC                      <  SRR4302537.4527498/94‑1 (MQ=60)
                                                                         GCTGGCTGGCCATCTTCTGCGGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTAC                      <  SRR4302537.4133274/94‑1 (MQ=60)
                                                                          CTGGCTGGCCATTTTCTGCGGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACG                     <  SRR4302537.5078536/94‑1 (MQ=60)
                                                                           TGGCTGGCCATTTTCTGCGGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGA                    <  SRR4302537.2779333/94‑1 (MQ=60)
                                                                            GGCTGGCCATTTTCTGCGGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGAC                   <  SRR4302537.1141711/94‑1 (MQ=60)
                                                                            GGCTGGCCATTTTCTGCGGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGAC                   <  SRR4302537.3583262/94‑1 (MQ=60)
                                                                             GCTGGCCATTTTCTGCGGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACT                  <  SRR4302537.1421821/94‑1 (MQ=60)
                                                                               TGGCCATTTTCTGCGGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTGG                >  SRR4302537.4455260/1‑94 (MQ=60)
                                                                               TGGCCATTTTCTGCGGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTGG                >  SRR4302537.4003415/1‑94 (MQ=60)
                                                                               TGGCCATTTTCTGCGGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTGG                >  SRR4302537.6148411/1‑94 (MQ=60)
                                                                                GGCCATTTTCTGCGGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTGGC               >  SRR4302537.6098496/1‑94 (MQ=60)
                                                                                 GCCATTTTCTGCGGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTGGCC              <  SRR4302537.5192006/94‑1 (MQ=60)
                                                                                   CATTTTCTGCGGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTGGCCGG            >  SRR4302537.1671000/1‑94 (MQ=60)
                                                                                   CATTTTCTGCGGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTGGCCGG            <  SRR4302537.3332171/94‑1 (MQ=60)
                                                                                    ATTTTCTGCGGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTGGCCGGG           >  SRR4302537.921701/1‑94 (MQ=60)
                                                                                    ATTTTCTGCGGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTGGCCGGG           >  SRR4302537.4167638/1‑94 (MQ=60)
                                                                                     TTTTCTGCGGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTGGCCGGGA          >  SRR4302537.4584154/1‑94 (MQ=60)
                                                                                       TTCTGCGGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTGGCCGGGAAA        <  SRR4302537.2052383/94‑1 (MQ=60)
                                                                                        TCTGCGGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTGGCCGGGAAAT       <  SRR4302537.5340758/94‑1 (MQ=60)
                                                                                        TCTGCGGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTGGCCGGGAAAT       <  SRR4302537.3844963/94‑1 (MQ=60)
                                                                                        TCTGCGGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTGGCCGGGAAAT       >  SRR4302537.640218/1‑94 (MQ=60)
                                                                                          TGCGGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTGGCAGGGAAATAT     >  SRR4302537.3304642/1‑94 (MQ=60)
                                                                                            CGGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTGGCCGGGAAATATTC   <  SRR4302537.2178791/94‑1 (MQ=60)
                                                                                             GGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTGGCCGGGAAATATTCG  >  SRR4302537.786474/1‑94 (MQ=60)
                                                                                             GGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTGGCCGGGAAATATTCG  <  SRR4302537.1558104/94‑1 (MQ=60)
                                                                                             GGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTGGCCGGGAAATATTCG  >  SRR4302537.5323014/1‑94 (MQ=60)
                                                                                             GGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTGGCCGGGAAATATTCG  >  SRR4302537.1871874/1‑94 (MQ=60)
                                                                                             |                                                                                             
ATCTCTACTATCGCCTGAATGTGGTGGCGATTGAAGTACCATCGCTGCGGCAACGGCGGGAAGATATCCCTCTGCTGGCTGGCCATTTTCTGCAGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTGGCCGGGAAATATTCG  >  NC_000913/4201787‑4201973

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑