Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 882 254.4 12957588 98.0% 12698436 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,224,576 A→G Y1057C (TAC→TGC)  metH → B12‑dependent homocysteine‑N5‑methyltetrahydrofolate transmethylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,224,5760AG95.3% 815.3 / 21.4 258Y1057C (TAC→TGC) metHB12‑dependent homocysteine‑N5‑methyltetrahydrofolate transmethylase
Reads supporting (aligned to +/- strand):  ref base A (6/6);  new base G (118/128);  total (124/134)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.26e-01

Only 100 of 261 total aligned reads displayed.

CACCATCTGCGTCAACAGACCGAAAAAACAGGCTTCGCTAACTACTGTCTCGCTGACTTCGTTGCGCCGAAGCTTTCTGGTAAAGCAGATTACATCGGCGCATTTGCCGTGACTGGCGGGCTGGAAGAGGACGCACTGGCTGATGCCTTTGAAGCGCAGCACGATGATTACAACAAAATCA  >  NC_000913/4224485‑4224665
                                                                                           |                                                                                         
caccaTCTGCGTCAACAGACCGAAAAAACAGGCTTCGCTAACTACTGTCTCGCTGACTTCGTTGCGCCGAAGCTTTCTGGTAAAGCAGATTAc                                                                                          <  1:6016573/93‑1 (MQ=255)
 accaTCTGCGTCAACAGACCGAAAAAGCAGGCTTCGCTAACTACTGTCTCGCTGATTTCGTTGCGCCGAAGCTTTCTGGTAAAGCAGATTGCa                                                                                         <  2:3867914/93‑1 (MQ=255)
 accaTCTGCGTCAACAGACCGAAAAAACAGGCTTCGCTAACTACTGTCTCGCTGACTTCGTTGCGCCGAAGCTTTCTGGTAAAGCAGATTGCa                                                                                         >  2:5768060/1‑93 (MQ=255)
 accaTCTGCGTCAACAGACCGAAAAAACAGGCTTCGCTAACTACTGTCTCGCTGACTTCGTTGCGCCGAAGCTTTCTGGTAAAGCAGATTGCa                                                                                         <  1:1294050/93‑1 (MQ=255)
  ccaTCTGCGTCAACAGACCGAAAAAACAGGCTTCGCTAACTACTGTCTCGCTGACTTCGTTGCGCCGAAGCTTTCTGGTAAAGCAGATTGCAt                                                                                        >  2:591824/1‑93 (MQ=255)
  ccaTCTGCGTCAACAGACCGAAAAAACAGGCTTCGCTAACTACTGTCTCGCTGACTTCGTTGCGCCGAAGCTTTCTGGTAAAGCAGATTGCAt                                                                                        <  2:6181652/93‑1 (MQ=255)
  ccaTCTGCGTCAACAGACCGAAAAAACAGGCTTCGCTAACTACTGTCTCGCTGACTTCGTTGCGCCGAAGCTTTCTGGTAAAGCAGATTGCAt                                                                                        <  1:1512686/93‑1 (MQ=255)
    aTCTGCGTCAACAGACCGAAAAAACAGGCTTCGCTAACTACTGTCTCGCTGACTTCGTTGCGCCGAAGCTTTCTGGTAAAGCAGATTGCATCg                                                                                      >  1:54326/1‑93 (MQ=255)
    aTCTGCGTCAACAGACCGAAAAAACAGGCTTCGCTAACTACTGTCTCGCTGACTTCGTTGCGCCGAAGCTTTCTGGTAAAGCAGATTGCATCg                                                                                      <  1:2164313/93‑1 (MQ=255)
    aTCTGCGTCAACAGACCGAAAAAACAGGCTTCGCTAACTACTGTCTCGCTGACTTCGTTGCGCCGAAGCTTTCTGGTAAAGCAGATTGCATCg                                                                                      >  1:6442395/1‑93 (MQ=255)
     tCTGCGTCAACAGACCGAAAAAACAGGCTTCGCTAACTACTGTCTCGCTGACTTCGTTGCGCCGAAGCTTTCTGGTAAAGCAGATTGCATCgg                                                                                     >  2:829743/1‑93 (MQ=255)
      cTGCGTCAACAGACCGAAAAAACAGGCTTCGCTAACTACTGTCTCGCTGACTTCGTTGCGCCGAAGCTTTCTGGTAAAGCAGATTGCATCGgc                                                                                    >  2:6177394/1‑93 (MQ=255)
        gCGTCAACAGACCGAAAAAACAGGCTTCGCTAACTACTGTCTCGCTGACTTCGTTGCGCCGAAGCTTTCTGGTAAAGCAGATTGCATCGgcgc                                                                                  >  1:770826/1‑93 (MQ=255)
              aCAGACCGAAAAAACAGGCTTCGCTAACTACTGTCTCGCTGACTTCGTTGCGCCGAAGCTTTCTGGTAAAGCAGATTGCATCGGCGCATTTGc                                                                            >  1:2072454/1‑93 (MQ=255)
                 gACCGAAAAAACAGGCTTCGCTAACTACTGTCTCGCTGACTTCGTTGCGCCGAAGCTTTCTGGTAAAGCAGATTGCATCGGCGCATTTGCCGt                                                                         >  1:2638596/1‑93 (MQ=255)
                 gACCGAAAAAACAGGCTTCGCTAACTACTGTCTCGCTGACTTCGTTGCGCCGAAGCTTTCTGGTAAAGCAGATTGCATCGGCGCATTTGCCGt                                                                         <  1:3977816/93‑1 (MQ=255)
                  aCCGAAAAAACAGGCTTCGCTAACTACTGTCTCGCTGACTTCGTTGCGCCGAAGCTTTCTGGTAAAGCAGATTGCATCGGCGCATTTGCCGTg                                                                        >  2:5521263/1‑93 (MQ=255)
                  aCCGAAAAAACAGGCTTCGCTAACTACTGTCTCGCTGACTTCGTTGCGCCGAAGCTTTCTGGTAAAGCAGATTGCATCGGCGCATTTGCCGTg                                                                        >  2:6373046/1‑93 (MQ=255)
                  aCCGAAAAAACAGGCTTCGCTAACTACTGTCTCGCTGACTTCGTTGCGCCGAAGCTTTCTGGTAAAGCAGATTGCATCGGCGCATTTGCCGTg                                                                        >  2:1128871/1‑93 (MQ=255)
                   ccGAAAAAACAGGCTTCGCTAACTACTGTCTCGCTGACTTCGTTGCGCCGAAGCTTTCTGGTAAAGCAGATTACATCGGCGCATTTGCCGTGa                                                                       <  1:3357892/93‑1 (MQ=255)
                    cGAAAAAACAGGCTTCGCTAACTACTGTCTCGCTGACTTCGTTGCGCCGAAGCTTTCTGGTAAAGCAGATTGCATCGGCGCATTTGCCGTGAc                                                                      >  1:5774265/1‑93 (MQ=255)
                    cGAAAAAACAGGCTTCGCTAACTACTGTCTCGCTGACTTCGTTGCGCCGAAGCTTTCTGGTAAAGCAGATTGCATCGGCGCATTTGCCGTGAc                                                                      <  2:885246/93‑1 (MQ=255)
                      aaaaaaCAGGCTTCGCTAACTACTGTCTCGCTGACTTCGTTGCGCCGAAGCTTTCTGGTAAAGCAGATTGCATCGGCGCATTTGCCGTGACTg                                                                    <  1:5259888/93‑1 (MQ=255)
                       aaaaaCAGGCTTCGCTAACTACTGTCTCGCTGACTTCGTTGCGCCGAAGCTTTCTGGTAAAGCAGATTGCATCGGCGCATTTGCCGTGACTgg                                                                   <  1:3281663/93‑1 (MQ=255)
                       aaaaaCAGGCTTCGCTAACTACTGTCTCGCTGACTTCGTTGCGCCGAAGCTTTCTGGTAAAGCAGATTGCATCGGCGCATTTGCCGTGACTgg                                                                   >  1:2692151/1‑93 (MQ=255)
                       aaaaaCAGGCTTCGCTAACTACTGTCTCGCTGACTTCGTTGCGCCGAAGCTTTCTGGTAAAGCAGATTGCATCGGCGCATTTGCCGTGACTgg                                                                   >  2:3528142/1‑93 (MQ=255)
                       aaaaaCAGGCTTCGCTAACTACTGTCTCGCTGACTTCGTTGCGCCGAAGCTTTCTGGTAAAGCAGATTGCATCGGCGCATTTGCCGTGACTgg                                                                   <  1:1894646/93‑1 (MQ=255)
                         aaaCAGGCTTCGCTAACTACTGTCTCGCTGACTTCGTTGCGCCGAAGCTTTCTGGTAAAGCAGATTGCATCGGCGCATTTGCCGTGACTGGCg                                                                 <  1:625928/93‑1 (MQ=255)
                         aaaCAGGCTTCGCTAACTACTGTCTCGCTGACTTCGTTGCGCCGAAGCTTTCTGGTAAAGCAGATTGCATCGGCGCATTTGCCGTGACTGGCg                                                                 >  2:2718759/1‑93 (MQ=255)
                         aaaCAGGCTTCGCTAACTACTGTCTCGCTGACTTCGTTGCGCCGAAGCTTTCTGGTAAAGCAGATTGCATCGGCGCATTTGCCGTGACTGGCg                                                                 >  2:1646673/1‑93 (MQ=255)
                          aaCAGGCTTCGCTAACTACTGTCTCGCTGACTTCGTTGCGCCGAAGCTTTCTGGTAAAGCAGATTGCATCGGCGCATTTGCCGTGACTGGCgg                                                                <  2:1309182/93‑1 (MQ=255)
                            cAGGCTTCGCTAACTACTGTCTCGCTGACTTCGTTGCGCCGAAGCTTTCTGGTAAAGCAGATTGCATCGGCGCATTTGCCGTGACTGGCGGGc                                                              <  1:5826881/93‑1 (MQ=255)
                            cAGGCTTCGCTAACTACTGTCTCGCTGACTTCGTTGCGCCGAAGCTTTCTGGTAAAGCAGATTGCATCGGCGCATTTGCCGTGACTGGCGGGc                                                              <  2:3793478/93‑1 (MQ=255)
                            cAGGCTTCGCTAACTACTGTCTCGCTGACTTCGTTGCGCCGAAGCTTTCTGGTAAAGCAGATTGCATCGGCGCATTTGCCGTGACTGGCGGGc                                                              <  2:5599747/93‑1 (MQ=255)
                             aGGCTTCGCTAACTACTGTCTCGCTGACTTCGTTGCGCCGAAGCTTTCTGGTAAAGCAGATTGCATCGGCGCATTTGCCGTGACTGGCGGGCt                                                             >  2:4056646/1‑93 (MQ=255)
                             aGGCTTCGCTAACTACTGTCTCGCTGACTTCGTTGCGCCGAAGCTTTCTGGTAAAGCAGATTGCATCGGCGCATTTGCCGTGACTGGCGGGCt                                                             <  2:4603939/93‑1 (MQ=255)
                             aGGCTTCGCTAACTACTGTCTCGCTGACTTCGTTGCGCCGAAGCTTTCTGGTAAAGCAGATTGCATCGGCGCATTTGCCGTGACTGGCGGGCt                                                             <  2:124892/93‑1 (MQ=255)
                              ggCTTCGCTAACTACTGTCTCGCTGACTTCGTTGCGCCGAAGCTTTCTGGTAAAGCAGATTGCATCGGCGCATTTGCCGTGACTGGCGGGCTg                                                            >  1:5602858/1‑93 (MQ=255)
                              ggCTTCGCTAACTACTGTCTCGCTGACTTCGTTGCGCCGAAGCTTTCTGGTAAAGCAGATTGCATCGGCGCATTTGCCGTGACTGGCGGGCTg                                                            <  2:5470060/93‑1 (MQ=255)
                                 ttCGCTAACTACTGTCTCGCTGACTTCGTTGCGCCGAAGCTTTCTGGTAAAGCAGATTGCATCGGCGCATTTGCCGTGACTGGCGGGCTGGaa                                                         <  1:42067/93‑1 (MQ=255)
                                 ttCGCTAACTACTGTCTCGCTGACTTCGTTGCGCCGAAGCTTTCTGGTAAAGCAGATTGCATCGGCGCATTTGCCGTGACTGGCGGGCTGGaa                                                         >  1:6302785/1‑93 (MQ=255)
                                 ttCGCTAACTACTGTCTCGCTGACTTCGTTGCGCCGAAGCTTTCTGGTAAAGCAGATTGCATCGGCGCATTTGCCGTGACTGGCGGGCTGGaa                                                         <  2:6224504/93‑1 (MQ=255)
                                  tCGCTAACTACTGTCTCGCTGACTTCGTTGCGCCGAAGCTTTCTGGTAAAGCAGATTGCATCGGCGCATTTGCCGTGACTGGCGGGCTGGAag                                                        <  2:1902616/93‑1 (MQ=255)
                                    gCTAACTACTGTCTCGCTGACTTCGTTGCGCCGAAGCTTTCTGGTAAAGCAGATTGCATCGGCGCATTTGCCGTGACTGGCGGGCTGGGagcg                                                      >  2:609098/1‑91 (MQ=255)
                                    gCTAACTACTGTCTCGCTGACTTCGTTGCGCCGAAGCTTTCTGGTAAAGCAGATTGCATCGGCGCATTTGCCGTGACTGGCGGGCTGGAagag                                                      >  2:323276/1‑93 (MQ=255)
                                    gCTAACTACTGTCTCGCTGACTTCGTTGCGCCGAAGCTTTCTGGTAAAGCAGATTGCATCGGCGCATTTGCCGTGACTGGCGGGCTGGAagag                                                      >  2:29796/1‑93 (MQ=255)
                                    gCTAACTACTGTCTCGCTGACTTCGTTGCGCCGAAGCTTTCTGGTAAAGCAGATTACATCGGCGCATTTGCCGTGACTGGCGGGCTGGAagag                                                      >  1:3066260/1‑93 (MQ=255)
                                     cTAACTACTGTCTCGCTGACTTCGTTGCGCCGAAGCTTTCTGGTAAAGCAGATTGCATCGGCGCATTTGCCGTGACTGGCGGGCTGGAAGAgg                                                     >  1:722701/1‑93 (MQ=255)
                                      tAACTACTGTCTCGCTGACTTCGTTGCGCCGAAGCTTTCTGGTAAAGCAGATTGCATCGGCGCATTTGCCGTGACTGGCGGGCTGGGAGAGGa                                                    >  1:2351899/1‑93 (MQ=255)
                                      tAACTACTGTCTCGCTGACTTCGTTGCGCCGAAGCTTTCTGGTAAAGCAGATTGCATCGGCGCATTTGCCGTGACTGGCGGGCTGGAAGAGGa                                                    <  1:1704614/93‑1 (MQ=255)
                                      tAACTACTGTCTCGCTGACTTCGTTGCGCCGAAGCTTTCTGGTAAAGCAGATTGCATCGGCGCATTTGCCGTGACTGGCGGGCTGGAAGAGGa                                                    <  1:812473/93‑1 (MQ=255)
                                      tAACTACTGTCTCGCTGACTTCGTTGCGCCGAAGCTTTCTGGTAAAGCAGATTACATCGGCGCATTTGCCGTGACTGGCGGGCTGGAAGAGGa                                                    >  2:6031807/1‑93 (MQ=255)
                                       aaCTAATGTCTCGCTGACTTCGTTGCGCCGCAGCTTTCTGGTAAAGCAGATTGCATCGGCGCAGTTGCCGTGACTGGCGGGCTGGAAGAGGAc                                                   <  1:3802316/93‑1 (MQ=255)
                                          tactGTCTCGCTGACTTCGTTGCGCCGAAGCTTTCTGGTAAAGCAGATTGCATCGGCGCATTTGCCGTGACTGGCGGGCTGGAAGAGGACGCa                                                >  1:5940480/1‑93 (MQ=255)
                                           actGTCTCGCTGACTTCGTTGCGCCGAAGCTTTCTGGTAAAGCAGATTGCATCGGCGCATTTGCCGTGACTGGCGGGCTGGGAGAGGGCGCAc                                               >  2:503934/1‑93 (MQ=255)
                                             tGTCTCGCTGACTTCGTTGCGCCGAAGCTTTCTGGTAAAGCAGATTGCATCGGCGCATTTGCCGTGACTGGCGGGCTGGAAGAGGACGCACTg                                             <  2:2424244/93‑1 (MQ=255)
                                               tctcGCTGACTTCGTTGCGCCGAAGCTTTCTGGTAAAGCAGATTGCATCGGCGCATTTGCCGTGACTGGCGGGCTGGAAGAGGACGCACTGGc                                           <  2:4638796/93‑1 (MQ=255)
                                               tctcGCTGACTTCGTTGCGCCGAAGCTTTCTGGTAAAGCAGATTGCATCGGCGCATTTGCCGTGACTGGCGGGCTGGAAGAGGACGCACTGGc                                           <  1:1111182/93‑1 (MQ=255)
                                                  cGCTGACTTCGTTGCGCCGAAGCTTTCTGGTAAAGCAGATTGCATCGGCGCATTTGCCGTGACTGGCGGGCTGGAAGAGGACGCACTGGCTGa                                        <  1:1101688/93‑1 (MQ=255)
                                                   gCTGACTTCGTTGCGCCGAAGCTTTCTGGTAAAGCAGATTGCATCGGCGCATTTGCCGTGACTGGCGGGCTGGAAGAGGACGCACTGGCTGAt                                       <  1:2855825/93‑1 (MQ=255)
                                                   gCTGACTTCGTTGCGCCGAAGCTTTCTGGTAAAGCAGATTGCATCGGCGCATTTGCCGTGACTGGCGGGCTGGAAGAGGACGCACTGGCTGAt                                       >  2:5207204/1‑93 (MQ=255)
                                                   gCTGACTTCGTTGCGCCGAAGCTTTCTGGTAAAGCAGATTGCATCGGCGCATTTGCCGTGACTGGCGGGCTGGAAGAGGACGCACTGGCTGAt                                       <  1:5628116/93‑1 (MQ=255)
                                                    cTGACTTCGTTGCGCCGAAGCTTTCTGGTAAAGCAGATTGCATCGGCGCATTTGCCGTGACTGGCGGGCTGGAAGAGGACGCACTGGCTGATg                                      <  1:4780680/93‑1 (MQ=255)
                                                    cTGACTTCGTTGCGCCGAAGCTTTCTGGTAAAGCAGATTGCATCGGCGCATTTGCCGTGACTGGCGGGCTGGAAGAGGACGCACTGGCTGATg                                      <  1:28082/93‑1 (MQ=255)
                                                    cTGACTTCGTTGCGCCGAAGCTTTCTGGTAAAGCAGATTGCATCGGCGCATTTGCCGTGACTGGCGGGCTGGAAGAGGACGCACTGGCTGATg                                      >  2:4542576/1‑93 (MQ=255)
                                                    cTGACTTCGTTGCGCCGAAGCTTTCTGGTAAAGCAGATTGCATCGGCGCATTTGCCGTGACTGGCGGGCTGGAAGAGGACGCACTGGCTGATg                                      <  2:3411426/93‑1 (MQ=255)
                                                      gACTTCGTTGCGCCGAAGCTTTCTGGTAAAGCAGATTGCATCGGCGCATTTGCCGTGACTGGCGGGCTGGGAGAGGACGCACTGGCTGATGcc                                    >  1:4436082/1‑93 (MQ=255)
                                                      gACTTCGTTGCGCCGAAGCTTTCTGGTAAAGCAGATTGCATCGGCGCATTTGCCGTGACTGGCGGGCTGGAAGAGGACGCACTGGCTGATGcc                                    >  1:5107949/1‑93 (MQ=255)
                                                      gACTTCGTAGCGCCGAAGCTTTCTGGTAAAGCAGATTGCATCGGCGCATTTGCCGTGACTGGCGGGCTGGAAGAGGACGCACTGGCTGATGcc                                    >  2:6293665/1‑93 (MQ=255)
                                                       aCTTCGTTGCGCCGAAGCTTTCTGGTAAAGCAGATTGCATCGGCGCATTTGCCGTGACTGGCGGGCTGGAAGAGGACGCACTGGCTGATGCCt                                   >  1:3138476/1‑93 (MQ=255)
                                                        cTTCGTTGCGCCGAAGCTTTCTGGTAAAGCAGATTGCATCGGCGCATTTGCCGTGACTGGCGGGCTGGAAGAGGACGCCCTGGCTGATGCCtt                                  >  1:2497140/1‑93 (MQ=255)
                                                        cTTCGTTGCGCCGAAGCTTTCTGGTAAAGCAGATTGCATCGGCGCATTTGCCGTGACTGGCGGGCTGGAAGAGGACGCACTGGCTGATGCCtt                                  <  1:4967108/93‑1 (MQ=255)
                                                        cTTCGTTGCGCCGAAGCTTTCTGGTAAAGCAGATTGCATCGGCGCATTTGCCGTGACTGGCGGGCTGGAAGAGGACGCACTGGCTGATGCCtt                                  <  2:4894494/93‑1 (MQ=255)
                                                         ttCGTTGCGCCGAAGCTTTCTGGTAAAGCAGATTACATCGGCGCATTTGCCGTGACTGGCGGGCTGGAAGAGGACGCACTGGCTGATGCCttt                                 >  1:4355580/1‑93 (MQ=255)
                                                           cGTTGCGCCGAAGCTTTCTGGTAAAGCAGATTGCATCGGCGCATTTGCCGTGACTGGCGGGCTGGAAGAGGACGCACTGGCTGATGCCTTTGa                               >  2:3156694/1‑93 (MQ=255)
                                                              tGCGCCGAAGCTTTCTGGTAAAGCAGATTGCATCGGCGCATTTGCCGTGACTGGCGGGCTGGAAGAGGACGCACTGGCTGATGCCTTTGAAgc                            <  1:404506/93‑1 (MQ=255)
                                                              tGCGCCGAAGCTTTCTGGTAAAGCAGATTGCATCGGCGCATTTGCCGTGACTGGCGGGCTGGAAGAGGACGCACTGGCTGATGCCTTTGAAgc                            <  1:4106830/93‑1 (MQ=255)
                                                                 gcCGAAGCTTTCTGGTAAAGCAGATTGCATCGGCGCATTTGCCGTGACTGGCGGGCTGGAAGAGGACGCACTGGCTGATGCCTTTGAAGCgca                         <  1:2394834/93‑1 (MQ=255)
                                                                    gAAGCTTTCTGGTAAAGCAGATTGCATCGGCGCATTTGCCGTGACTGGCGGGCTGGAAGAGGACGCACTGGCTGATGCCTTTGAAGCgcagca                      >  2:3963386/1‑93 (MQ=255)
                                                                     aaGCTTTCTGGTAAAGCAGATTGCATCGGCGCATTTGCCGTGACTGGCGGGCTGGAAGAGGACGCACTGGCTGATGCCTTTGAAGCGCAGCAc                     <  1:3543874/93‑1 (MQ=255)
                                                                     aaGCTTTCTGGTAAAGCAGATTGCATCGGCGCATTTGCCGTGACTGGCGGGCTGGAAGAGGACGCACTGGCTGATGCCTTTGAAGCGCAGCAc                     >  1:369858/1‑93 (MQ=255)
                                                                       gCTTTCTGGTAAAGCAGATTGCATCGGCGCATTTGCCGTGACTGGCGGGCTGGAAGAGGACGCACTGGCTGATGCCTTTGAAGCGCAGCACga                   <  1:1207313/93‑1 (MQ=255)
                                                                        cTTTCTGGTAAAGCAGATTGCATCGGCGCATTTGCCGTGACTGGCGGGCTGGAAGAGGACGCACTGGCTGATGCCTTTGAAGCGCAGCACgat                  <  1:272859/93‑1 (MQ=255)
                                                                        cTTTCTGGTAAAGCAGATTACATCGGCGCATTTGCCGTGACTGGCGGGCTGGAAGAGGACGCACTGGCTGATGCCTTTGAAGCGCAGCACgat                  >  2:4309642/1‑93 (MQ=255)
                                                                          ttCTGGTAAAGCAGATTGCATCGGCGCATTTGCCGTGACTGGCGGGCTGGAAGAGGACGCACTGGCTGATGCCTTTGCAGCGCCGCACgatga                >  2:675399/1‑93 (MQ=255)
                                                                          ttCTGGTAAAGCAGATTGCATCGGCGCATTTGCCGTGACTGGCGGGCTGGAAGAGGACGCACTGGCTGATGCCTTTGAAGCGCAGCACgatga                <  2:6396682/93‑1 (MQ=255)
                                                                          ttCTGGTAAAGCAGATTGCATCGGCGCATTTGCCGTGACTGGCGGGCTGGAAGAGGACGCACTGGCTGATGCCTTTGAAGCGCAGCACgatga                <  2:943810/93‑1 (MQ=255)
                                                                           tCTGGTAAAGCAGATTGCATCGGCGCATTTGCCGTGACTGGCGGGCTGGAAGAGGACGCACTGGCTGATGCCTTTGAAGCGCAGCACgatgat               >  2:2520253/1‑93 (MQ=255)
                                                                            cTGGTAAAGCAGATTGCATCGGCGCATTTGCCGTGCCTGGCGGGCTGGAAGAGGACGCACTGGCTGATGCCTTTGAAGCGCAGCACGATGAtt              <  1:6223504/93‑1 (MQ=255)
                                                                            cTGGTAAAGCAGATTGCATCGGCGCATTTGCCGTGACTGGCGGGCTGGAAGAGGACGCACTGGCTGATGCCTTTGAAGCGCAGCACGATGAtt              <  1:4370162/93‑1 (MQ=255)
                                                                            cTGGTAAAGCAGATTGCATCGGCGCATTTGCCGTGACTGGCGGGCTGGAAGAGGACGCACTGGCTGATGCCTTTGAAGCGCAGCACGATGAtt              >  2:1027296/1‑93 (MQ=255)
                                                                             tGGTAAAGCAGATTGCATCGGCGCATTTGCCGTGACTGGCGGGCTGGAAGAGGACGCACTGGCTGATGCCTTTGAAGCGCAGCACGATGATTa             >  1:4683645/1‑93 (MQ=255)
                                                                             tGGTAAAGCAGATTGCATCGGCGCATTTGCCGTGACTGGCGGGCTGGAAGAGGACGCACTGGCTGATGCCTTTGAAGCGCAGCACGATGATTa             >  2:2211543/1‑93 (MQ=255)
                                                                              ggTAAAGCAGATTGCATCGGCGCATTTGCCGTGACTGGCGGGCTGGAAGAGGACGCACTGGCTGATGCCTTTGAAGCGCAGCACGATGATTac            <  2:5329795/93‑1 (MQ=255)
                                                                               gTAAAGCAGATTGCATCGGCGCATTTGCCGTGACTGGCGGGCTGGAAGAGGACGCACTGGCTGATGCCTTTGAAGCGCAGCACGATGATTaca           <  1:5510463/93‑1 (MQ=255)
                                                                                tAAAGCAGATTGCATCGGCGCATTTGCCGTGACTGGCGGGCTGGAAGAGGACGCACTGGCTGATGCCTTTGAAGCGCAGCACGATGATTacaa          <  2:779956/93‑1 (MQ=255)
                                                                                  aaGCAGATTGCATCGGCGCATTTGCCGTGACTGGCGGGCTGGAAGAGGACGCACTGGCTGATGCCTTTGAAGCGCAGCACGATGATTacaaca        >  2:3573881/1‑93 (MQ=255)
                                                                                     cAGATTGCATCGGCGCATTTGCCGTGACTGGCGGGCTGGAAGAGGACGCACTGGCTGATGCCTTTGAAGCGCAGCACGATGATTACAACaaaa     <  2:1981209/93‑1 (MQ=255)
                                                                                        aTTGCATGGGCGCATTTGCCGTGACTGGCGGGCTGGGAGAGGACGCACTGGCTGATGCCTTTGAAGCGCAGCACGAAGATTACAACACAATCa  >  2:5233775/1‑93 (MQ=255)
                                                                                        aTTGCAGCGCCGCATTTGCCGTGACTGGCGGGCTGGAAGAGGACGCACTGGCTGATGCCTTTGAAGCGCAGCACGATGATTACAACAAAATCa  <  2:2114934/93‑1 (MQ=255)
                                                                                           |                                                                                         
CACCATCTGCGTCAACAGACCGAAAAAACAGGCTTCGCTAACTACTGTCTCGCTGACTTCGTTGCGCCGAAGCTTTCTGGTAAAGCAGATTACATCGGCGCATTTGCCGTGACTGGCGGGCTGGAAGAGGACGCACTGGCTGATGCCTTTGAAGCGCAGCACGATGATTACAACAAAATCA  >  NC_000913/4224485‑4224665

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GCCACCATCTGCGTCAACAGACCGAAAAAACAGGCTTCGCTAACTACTGTCTCGCTGACTTCGTTGCGCCGAAGCTTTCTGGTAAAGCAGATTACATCGGCGCATTTGCCGTGACTGGCGGGCTGGAAGAGGACGCACTGGCTGATGCCTTTGAAGCGCAGCACGATGATTACAACAAAATCATGGT  >  NC_000913/4224483‑4224669
                                                                                             |                                                                                             
GCCACCATCTGCGTCAACAGACCGAAAAAACAGGCTTCGCTAACTACTGTCTCGCTGACTTCGTTGCGCCGAAGCTTTCTGGTAAAGCAGATTG                                                                                               <  SRR4302537.388972/94‑1 (MQ=60)
GCCACCATCTGCGTCAACAGACCGAAAAAACAGGCTTCGCTAACTACTGTCTCGCTGACTTCGTTGCGCCGAAGCTTTCTGGTAAAGCAGATTG                                                                                               >  SRR4302537.3470629/1‑94 (MQ=60)
  CACCATCTGCGTCAACAGACCGAAAAAACAGGCTTCGCTAACTACTGTCTCGCTGACTTCGTTGCGCCGAAGCTTTCTGGTAAAGCAGATTGCA                                                                                             >  SRR4302537.3085148/1‑94 (MQ=60)
  CACCATCTGCGTCAACAGACCGAAAAAACAGGCTTCGCTAACTACTGTCTCGCTGACTTCGTTGCGCCGAAGCTTTCTGGTAAAGCAGATTACA                                                                                             <  SRR4302537.6057911/94‑1 (MQ=60)
   ACCATCTGCGTCAACAGACCGAAAAAACAGGCTTCGCTAACTACTGTCTCGCTGACTTCGTTGCGCCGAAGCTTTCTGGTAAAGCAGATTGCAT                                                                                            >  SRR4302537.279789/1‑94 (MQ=60)
   ACCATCTGCGTCAACAGACCGAAAAAACAGGCTTCGCTAACTACTGTCTCGCTGACTTCGTTGCGCCGAAGCTTTCTGGTAAAGCAGATTGCAT                                                                                            <  SRR4302537.906344/94‑1 (MQ=60)
   ACCATCTGCGTCAACAGACCGAAAAAACAGGCTTCGCTAACTACTGTCTCGCTGACTTCGTTGCGCCGAAGCTTTCTGGTAAAGCAGATTGCAT                                                                                            <  SRR4302537.1303047/94‑1 (MQ=60)
    CCATCTGCGTCAACAGACCGAAAAAACAGGCTTCGCTAACTACTGTCTCGCTGACTTCGTTGCGCCGAAGCTTTCTGGTAAAGCAGATTGCATC                                                                                           <  SRR4302537.1523257/94‑1 (MQ=60)
     CATCTGCGTCAACAGACCGAAAAAACAGGCTTCGCTAACTACTGTCTCGCTGACTTCGTTGCGCCGAAGCTTTCTGGTAAAGCAGATTGCATCG                                                                                          >  SRR4302537.6486112/1‑94 (MQ=60)
     CATCTGCGTCAACAGACCGAAAAAACAGGCTTCGCTAACTACTGTCTCGCTGACTTCGTTGCGCCGAAGCTTTCTGGTAAAGCAGATTGCATCG                                                                                          >  SRR4302537.2372626/1‑94 (MQ=60)
     CATCTGCGTCAACAGACCGAAAAAACAGGCTTCGCTAACTACTGTCTCGCTGACTTCGTTGCGCCGAAGCTTTCTGGTAAAGCAGATTGCATCG                                                                                          <  SRR4302537.6302477/94‑1 (MQ=60)
     CATCTGCGTCAACAGACCGAAAAAACAGGCTTCGCTAACTACTGTCTCGCTGACTTCGTTGCGCCGAAGCTTTCTGGTAAAGCAGATTGCATCG                                                                                          >  SRR4302537.54724/1‑94 (MQ=60)
     CATCTGCGTCAACAGACCGAAAAAACAGGCTTCGCTAACTACTGTCTCGCTGACTTCGTTGCGCCGAAGCTTTCTGGTAAAGCAGATTGCATCG                                                                                          <  SRR4302537.5583547/94‑1 (MQ=60)
      ATCTGCGTCAACAGACCGAAAAAACAGGCTTCGCTAACTACTGTCTCGCTGACTTCGTTGCGCCGAAGCTTTCTGGTAAAGCAGATTGCATCGG                                                                                         <  SRR4302537.2179561/94‑1 (MQ=60)
       TCTGCGTCAACAGACCGAAAAAACAGGCTTCGCTAACTACTGTCTCGCTGACTTCGTTGCGCCGAAGCTTTCTGGTAAAGCAGATTACATCGGC                                                                                        <  SRR4302537.1442298/94‑1 (MQ=60)
        CTGCGTCAACAGACCGAAAAAACAGGCTTCGCTAACTACTGTCTCGCTGACTTCGTTGCGCCGAAGCTTTCTGGTAAAGCAGATTGCATCGGCG                                                                                       <  SRR4302537.2172127/94‑1 (MQ=60)
         TGCGTCAACAGACCGAAAAAACAGGCTTCGCTAACTACTGTCTCGCTGACTTCGTTGCGCCGAAGCTTTCTGGTAAAGCAGATTGCATCGGCGC                                                                                      >  SRR4302537.776111/1‑94 (MQ=60)
          GCGTCAACAGACCGAAAAAACAGGCTTCGCTAACTACTGTCTCGCTGACTTCGTTGCGCCGAAGCTTTCTGGTAAAGCAGATTGCATCGGAGCA                                                                                     >  SRR4302537.1158673/1‑94 (MQ=60)
            GTCAACAGACCGAAAAAACAGGCTTCGCTAACTACTGTCTCGCTGACTTCGTTGCGCCGAAGCTTTCTGGTAAAGCAGATTGCATCGGCGCATT                                                                                   <  SRR4302537.3799051/94‑1 (MQ=60)
            GTCAACAGACCGAAAAAACAGGCTTCGCTAACTACTGTCTCGCTGACTTCGTTGCGCCGAAGCTTTCTGGTAAAGCAGATTGCATCGGCGCATT                                                                                   <  SRR4302537.6438479/94‑1 (MQ=60)
            GTCAACAGACCGAAAAAACAGGCTTCGCTAACTACTGTCTCGCTGACTTCGTTGCGCCGAAGCTTTCTGGTAAAGCAGATTGCATCGGCGCATT                                                                                   >  SRR4302537.765374/1‑94 (MQ=60)
             TCAACAGACCGAAAAAACAGGCTTCGCTAACTACTGTCTCGCTGACTTCGTTGCGCCGAAGCTTTCTGGTAAAGCAGATTGCATCGGCGCATTT                                                                                  >  SRR4302537.650995/1‑94 (MQ=60)
               AACAGACCGAAAAAACAGGCTTCGCTAACTACTGTCTCGCTGACTTCGTTGCGCCGAAGCTTTCTGGTAAAGCAGATTGCATCGGCGCATTTGC                                                                                >  SRR4302537.2087053/1‑94 (MQ=60)
                 CAGACCGAAAAAACAGGCTTCGCTAACTACTGTCTCGCTGACTTCGTTGCGCCGAAGCTTTCTGGTAAAGCAGATTGCATCGGCGCATTTGCCG                                                                              <  SRR4302537.1117987/94‑1 (MQ=60)
                  AGACCGAAAAAACAGGCTTCGCTAACTACTGTCTCGCTGACTTCGTTGCGCCGAAGCTTTCTGGTAAAGCAGATTGCATCGGCGCATTTGCCGT                                                                             >  SRR4302537.2657055/1‑94 (MQ=60)
                   GACCGAAAAAACAGGCTTCGCTAACTACTGTCTCGCTGACTTCGTTGCGCCGAAGCTTTCTGGTAAAGCAGATTGCATCGGCGCATTTGCCGTG                                                                            <  SRR4302537.4005656/94‑1 (MQ=60)
                    ACCGAAAAAACAGGCTTCGCTAACTACTGTCTCGCTGACTTCGTTGCGCCGAAGCTTTCTGGTAAAGCAGATTGCATCGGCGCATTTGCCGTGA                                                                           >  SRR4302537.3350368/1‑94 (MQ=60)
                     CCGAAAAAACAGGCTTCGCTAACTACTGTCTCGCTGACTTCGTTGCGCCGAAGCTTTCTGGTAAAGCAGATTGCATCGGCGCATTTGCCGTGAC                                                                          >  SRR4302537.5813842/1‑94 (MQ=60)
                     CCGAAAAAACAGGCTTCGCTAACTACTGTCTCGCTGACTTCGTTGCGCCGAAGCTTTCTGGTAAAGCAGATTGCATCGGCGCATTTGCCGTGAC                                                                          >  SRR4302537.6002619/1‑94 (MQ=60)
                     CCGAAAAAACAGGCTTCGCTAACTACTGTCTCGCTGACTTCGTTGCGCCGAAGCTTTCTGGTAAAGCAGATTACATCGGCGCATTTGCCGTGAC                                                                          <  SRR4302537.3381677/94‑1 (MQ=60)
                       GAAAAAACAGGCTTCGCTAACTACTGTCTCGCTGACTTCGTTGCGCCGAAGCTTTCTGGTAAAGCAGATTGCATCGGCGCATTTGCCGTGACTG                                                                        >  SRR4302537.1709168/1‑94 (MQ=60)
                        AAAAAACAGGCTTCGCTAACTACTGTCTCGCTGACTTCGTTGCGCCGAAGCTTTCTGGTAAAGCAGATTGCATCGGCGCATTTGCCGTGACTGG                                                                       >  SRR4302537.2710955/1‑94 (MQ=60)
                        AAAAAACAGGCTTCGCTAACTACTGTCTCGCTGACTTCGTTGCGCCGAAGCTTTCTGGTAAAGCAGATTGCATCGGCGCATTTGCCGTGACTGG                                                                       >  SRR4302537.2793011/1‑94 (MQ=60)
                        AAAAAACAGGCTTCGCTAACTACTGTCTCGCTGACTTCGTTGCGCCGAAGCTTTCTGGTAAAGCAGATTGCATCGGCGCATTTGCCGTGACTGG                                                                       >  SRR4302537.3291106/1‑94 (MQ=60)
                        AAAAAACAGGCTTCGCTAACTACTGTCTCGCTGACTTCGTTGCGCCGAAGCTTTCTGGTAAAGCAGATTGCATCGGCGCATTTGCCGTGACTGG                                                                       <  SRR4302537.5296079/94‑1 (MQ=60)
                         AAAAACAGGCTTCGCTAACTACTGTCTCGCTGACTTCGTTGCGCCGAAGCTTTCTGGTAAAGCAGATTGCATCGGCGCATTTGCCGTGACTGGC                                                                      <  SRR4302537.1907810/94‑1 (MQ=60)
                         AAAAACAGGCTTCGCTAACTACTGTCTCGCTGACTTCGTTGCGCCGAAGCTTTCTGGTAAAGCAGATTGCATCGGCGCATTTGCCGTGACTGGC                                                                      <  SRR4302537.3304830/94‑1 (MQ=60)
                          ACAACAGTCTTCGCTAACTACTGTCTCGCTGACTTCGTTGCGCCGAAGCTTTCTGGTAAAGCAGATTGCATCGGCGCATTTGCCGTGACTGGCG                                                                     <  SRR4302537.2647491/94‑1 (MQ=60)
                           AAACAGGCTTCGCTAACTACTGTCTCGCTGACTTCGTTGCGCCGAAGCTTTCTGGTAAAGCAGATTGCATCGGCGCATTTGCCGTGACTGGCGG                                                                    >  SRR4302537.3368907/1‑94 (MQ=60)
                           AAACAGGCTTCGCTAACTACTGTCTCGCTGACTTCGTTGCGCCGAAGCTTTCTGGTAAAGCAGATTGCATCGGCGCATTTGCCGTGACTGGCGG                                                                    <  SRR4302537.630194/94‑1 (MQ=60)
                            AACAGGCTTCGCTAACTACTGTCTCGCTGACTTCGTTGCGCCGAAGCTTTCTGGTAAAGCAGATTGCATCGGCGCATTTGCCGTGACTGGCGGG                                                                   <  SRR4302537.5187233/94‑1 (MQ=60)
                            AACAGGCTTCGCTAACTACTGTCTCGCTGACTTCGTTGCGCCGAAGCTTTCTGGTAAAGCAGATTGCATCGGCGCATTTGCCGTGACTGGCGGG                                                                   <  SRR4302537.6410769/94‑1 (MQ=60)
                              CAGGCTTCGCTAACTACTGTCTCGCTGACTTCGTTGCGCCGAAGCTTTCTGGTAAAGCAGATTGCATCGGCGCATTTGCCGTGACTGGCGGGCT                                                                 <  SRR4302537.5866817/94‑1 (MQ=60)
                               AGGCTTCGCTAACTACTGTCTCGCTGACTTCGTTGCGCCGAAGCTTTCTGGTAAAGCAGATTGCATCGGCGCATTTGCCGTGACTGGCGGGCTG                                                                >  SRR4302537.5641162/1‑94 (MQ=60)
                               AGGCTTCGCTAACTACTGTCTCGCTGACTTCGTTGCGCCGAAGCTTTCTGGTAAAGCAGATTGCATCGGCGCATTTGCCGTGACTGGCGGGCTG                                                                >  SRR4302537.2835430/1‑94 (MQ=60)
                               AGGCTTCGCTAACTACGGTCTCGCTGACTTCGTTGCGCCGAAGCTTTCTGGTAAAGCAGATTGCATCGGCGCATTTGCCGTGACTGGCGGGCTG                                                                <  SRR4302537.6296194/94‑1 (MQ=60)
                                GGCTTCGCTAACTACTGTCTCGCTGACTTCGTTGCGCCGAAGCTTTCTGGTAAAGCAGATTGCATCGGCGCATTTGCCGTGACTGGCGGGCTGG                                                               >  SRR4302537.627972/1‑94 (MQ=60)
                                GGCTTCGCTAACTACTGTCTCGCTGACTTCGTTGCGCCGAAGCTTTCTGGTAAAGCAGATTGCATCGGCGCATTTGCCGTGACTGGCGGGCTGG                                                               <  SRR4302537.4138671/94‑1 (MQ=60)
                                 GCTTCGCTAACTACTGTCTCGCTGACTTCGTTGCGCCGAAGCTTTCTGGTAAAGCAGATTGCATGGGCGCATTTGCCGTGACTGGCGGGCTGGA                                                              >  SRR4302537.2442721/1‑94 (MQ=60)
                                  CTTCGCTAACTACTGTCTCGCTGACTTCGTTGCGCCGAAGCTTTCTGGTAAAGCAGATTGCATCGGCGCATTTGCCGTGACTGGCGGGCTGGAA                                                             >  SRR4302537.6345949/1‑94 (MQ=60)
                                   TTCGCTAACTACTGTCTCGCTGACTTCGTTGCGCCGAAGCTTTCTGGTAAAGCAGATTGCATCGGCGCATTTGCCGTGACTGGCGGGCTGGAAG                                                            <  SRR4302537.42382/94‑1 (MQ=60)
                                   TTCGCTAACTACTGTCTCGCTGACTTCGGTGCGCCGAAGCTTTCTGGTAAAGCAGATTACATCGGCGCATTTGCCGTGACTGGCGGGCTGGAAG                                                            <  SRR4302537.4897804/94‑1 (MQ=60)
                                     CGCTAACTACTGTCTCGCTGACTTCGTTGCGCCGAAGCTTTCTGGTAAAGCAGATTGCATCGGCGCATTTGCCGTGACTGGCGGGCTGGAAGAG                                                          >  SRR4302537.3135455/1‑94 (MQ=60)
                                     CGCTAACTACTGTCTCGCTGACTTCGTTGCGCCGAAGCTTTCTGGTAAAGCAGATTGCATCGGCGCATTTGCCGTGACTGGCGGGCTGGAAGAG                                                          <  SRR4302537.4059844/94‑1 (MQ=60)
                                     CGCTAACTACTGTCTCGCTGACTTCGTTGCGCCGAAGCTTTCTGGTAAAGCAGATTGCATCGGCGCATTTGCCGTGACTGGCGGGCTGGAAGAG                                                          <  SRR4302537.534851/94‑1 (MQ=60)
                                     CGCTAACTACTGTCTCGCTGACTTCGTTGCGCCGAAGCTTTCTGGTAAAGCAGATTACATCGGCGCATTTGCCGTGACTGGCGGGCTGGAAGAG                                                          >  SRR4302537.3087625/1‑94 (MQ=60)
                                      GCTAACTACTGTCTCGCTGACTTCGTTGCGCCGAAGCTTTCTGGTAAAGCAGATTGCATCGGCGCATTTGCCGTGACTGGCGGGCTGGAAGAGG                                                         >  SRR4302537.727632/1‑94 (MQ=60)
                                       CTAACTACTGTCTCGCTGACTTCGTTGCGCCGAAGCTTTCTGGTAAAGCAGATTGCATCGGCGCATTTGCCGTGACTGGCGGGCTGGGAGAGGA                                                        >  SRR4302537.2368459/1‑94 (MQ=60)
                                        TAACTACTGTCTCGCTGACTTCGTTGCGCCGAAGCTTTCTGGTAAAGCAGATTGCATCGGCGCATTTGCCGTGACTGGCGGGCTGGAAGAGGAC                                                       >  SRR4302537.808632/1‑94 (MQ=60)
                                        TAACTACTGTCTCGCTGACTTCGTTGCGCCGAAGCTTTCTGGTAAAGCAGATTGCATCGGCGCATTTGCCGTGACTGGCGGGCTGGAAGAGGAC                                                       <  SRR4302537.1716530/94‑1 (MQ=60)
                                        TAACTACTGTCTCGCTGACTTCGTTGCGCCGAAGCTTTCTGGTAAAGCAGATTGCATCGGCGCATTTGCCGTGACTGGCGGGCTGGAAGAGGAC                                                       <  SRR4302537.818040/94‑1 (MQ=60)
                                         AACTACTGTCTCGCTGACTTCGTTGCGCCGAAGCTTTCTGGTAAAGCAGATTGCATCGGCGCATTTGCCGTGACTGGCGGGCTGGAAGAGGACG                                                      <  SRR4302537.4355434/94‑1 (MQ=60)
                                         AACTAATGTCTCGCTGACTTCGTTGCGCCGCAGCTTTCTGGTAAAGCAGATTGCATCGGCGCAGTTGCCGTGACTGGCGGGCTGGAAGAGGACG                                                      <  SRR4302537.3828987/94‑1 (MQ=60)
                                           CTACTGTCTCGCTGACTTCGTTGCGCCGAAGCTTTCTGGTAAAGCAGATTGCATCGGCGCATTTGCCGTGACTGGCGGGCTGGAAGAGGACGCA                                                    >  SRR4302537.5981253/1‑94 (MQ=60)
                                             ACTGTCTCGCTGACTTCGTTGCGCCGAAGCTTTCTGGTAAAGCAGATTGCATCGGCGCATTTGCCGTGACTGGCGGGCTGGAAGAGGACGCACT                                                  <  SRR4302537.2552522/94‑1 (MQ=60)
                                             ACTGTCTCGCTGACTTCGTTGCGCCGAAGCTTTCTGGTAAAGCAGATTGCATCGGCGCATTTGCCGTGACTGGCGGGCTGGAAGAGGACGCACT                                                  <  SRR4302537.5832465/94‑1 (MQ=60)
                                               TGTCTCGCTGACTTCGTTGCGCCGAAGCTTTCTGGTAAAGCAGATTGCATCGGCGCATTTGCCGTGACTGGCGGGCTGGAAGAGGACGCACTGG                                                >  SRR4302537.4759600/1‑94 (MQ=60)
                                               TGTCTCGCTGACTTCGTTGCGCCGAAGCTTTCTGGTAAAGCAGATTGCATCGGCGCATTTGCCGTGACTGGCGGGCTGGAAGAGGACGCACTGG                                                <  SRR4302537.3810150/94‑1 (MQ=60)
                                                 TCTCGCTGACTTCGTTGCGCCGAAGCTTTCTGGTAAAGCAGATTGCATCGGCGCATTTGCCGTGACTGGCGGGCTGGAAGAGGACGCACTGGCT                                              <  SRR4302537.1118856/94‑1 (MQ=60)
                                                 TCTCGCTGACTTCGTTGCGCCGAAGCTTTCTGGTAAAGCAGATTGCATCGGCGCATTTGCCGTGACTGGCGGGCTGGAAGAGGACGCACTGGCT                                              <  SRR4302537.2744816/94‑1 (MQ=60)
                                                    CGCTGACTTCGTTGCGCCGAAGCTTTCTGGTAAAGCAGATTGCATCGGCGCATTTGCCGTGACTGGCGGGCTGGAAGAGGACGCACTGGCTGAT                                           <  SRR4302537.1109301/94‑1 (MQ=60)
                                                    CGCTGACTTCGTTGCGCCGAAGCTTTCTGGTAAAGCAGATTACATCGGCGCATTTGCCGTGACTGGCGGGCTGGAAGAGGACGCACTGGCTGAT                                           >  SRR4302537.5263220/1‑94 (MQ=60)
                                                     GCTGACTTCGTTGCGCCGAAGCTTTCTGGTAAAGCAGATTGCATCGGCGCATTTGCCGTGACTGGCGGGCTGGAAGAGGACGCACTGGCTGATG                                          <  SRR4302537.5666619/94‑1 (MQ=60)
                                                     GCTGACTTCGTTGCGCCGAAGCTTTCTGGTAAAGCAGATTGCATCGGCGCATTTGCCGTGACTGGCGGGCTGGAAGAGGACGCACTGGCTGATG                                          >  SRR4302537.5663627/1‑94 (MQ=60)
                                                     GCTGACTTCGTTGCGCCGAAGCTTTCTGGTAAAGCAGATTGCATCGGCGCATTTGCCGTGACTGGCGGGCTGGAAGAGGACGCACTGGCTGATG                                          <  SRR4302537.2875845/94‑1 (MQ=60)
                                                      CTGACTTCGTTGCGCCGAAGCTTTCTGGTAAAGCAGATTGCATCGGCGCATTTGCCGTGACTGGCGGGCTGGAAGAGGACGCACTGGCTGATGC                                         <  SRR4302537.4813684/94‑1 (MQ=60)
                                                      CTGACTTCGTTGCGCCGAAGCTTTCTGGTAAAGCAGATTGCATCGGCGCATTTGCCGTGACTGGCGGGCTGGAAGAGGACGCACTGGCTGATGC                                         <  SRR4302537.28301/94‑1 (MQ=60)
                                                       TGACTTCGTTGCGCCGAAGCTTTCTGGTAAAGCAGATTGCATCGGCGCATTTGCCGTGACTGGCGGGCTGGGAGAGGACGCACTGGCTGATGCC                                        >  SRR4302537.4466712/1‑94 (MQ=60)
                                                       TGACTTCGTTGCGCCGAAGCTTTCTGGTAAAGCAGATTGCATCGGCGCATTTGCCGTGACTGGCGGGCTGGAAGAGGACGCACTGGCTGATGCC                                        >  SRR4302537.4672859/1‑94 (MQ=60)
                                                       TGACTTCGTTGCGCCGAAGCTTTCTGGTAAAGCAGATTGCATCGGCGCATTTGCCGTGACTGGCGGGCTGGAAGAGGACGCACTGGCTGATGCC                                        >  SRR4302537.5143148/1‑94 (MQ=60)
                                                        GACTTCGTTGCGCCGAAGCTTTCTGGTAAAGCAGATTGCATCGGCGCATTTGCCGTGACTGGCGGGCTGGAAGAGGACGCACTGGCTGATGCCT                                       <  SRR4302537.5537296/94‑1 (MQ=60)
                                                        GACTTCGTTGCGCCGAAGCTTTCTGGTAAAGCAGATTGCATCGGCGCATTTGCCGTGACTGGCGGGCTGGAAGAGGACGCACTGGCTGATGCCT                                       >  SRR4302537.3160502/1‑94 (MQ=60)
                                                        GACTTCGTTGCGCCGAAGCTTTCTGGTAAAGCAGATTGCATCGGCGCATTTGCCGTGACTGGCGGGCTGGAAGAGGACGCACTGGCTGATGCCT                                       >  SRR4302537.1270184/1‑94 (MQ=60)
                                                         ACTTCGTTGCGCCGAAGCTTTCTGGTAAAGCAGATTGCATCGGCGCATTTGCCGTGACTGGCGGGCTGGAAGAGGACGCCCTGGCTGATGCCTT                                      >  SRR4302537.2514716/1‑94 (MQ=60)
                                                         ACTTCGTTGCGCCGAAGCTTTCTGGTAAAGCAGATTGCATCGGCGCATTTGCCGTGACTGGCGGGCTGGAAGAGGACGCACTGGCTGATGCCTT                                      <  SRR4302537.4350655/94‑1 (MQ=60)
                                                         ACTTCGTTGCGCCGAAGCTTTCTGGTAAAGCAGATTGCATCGGCGCATTTGCCGTGACTGGCGGGCTGGAAGAGGACGCACTGGCTGATGCCTT                                      <  SRR4302537.6322800/94‑1 (MQ=60)
                                                          CTTCGTTGCGCCGAAGCTTTCTGGTAAAGCAGATTGCATCGGCGCATTTGCCGTGACTGGCGGGCTGGAAGAGGACGCACTGGCTGATGCCTTT                                     <  SRR4302537.6033736/94‑1 (MQ=60)
                                                          CTTCGTTGCGCCGAAGCTTTCTGGTAAAGCAGATTGCATCGGCGCATTTGCCGTGACTGGCGGGCTGGAAGAGGACGCACTGGCTGATGCCTTT                                     <  SRR4302537.5090552/94‑1 (MQ=60)
                                                          CTTCGTTGCGCCGAAGCTTTCTGGTAAAGCAGATTGCATCGGCGCATTTGCCGTGACTGGCGGGCTGGAAGAGGACGCACTGGCTGATGCCTTT                                     <  SRR4302537.1144846/94‑1 (MQ=60)
                                                          CTTCGTTGCGCCGAAGCTTTCTGGTAAAGCAGATTGCATCGGCGCATTTGCCGTGACTGGCGGGCTGGAAGAGGACGCACTGGCTGATGCCTTT                                     <  SRR4302537.5001344/94‑1 (MQ=60)
                                                          CTTCGTTGCGCCGAAGCTTTCTGGTAAAGCAGATTACATCGGCGCATTTGCCGTGACTGGCGGGCTGGAAGAGGACGCACTGGCTGATGCCTTT                                     >  SRR4302537.4385735/1‑94 (MQ=60)
                                                           TTCGTTGCGCCGATGCTTTCTGGTAAAGCAGATTGCATCGTCGCATTTGCCGTGACTGGCGGGCTGGAAGAGGACGCACTGGCTGATGCCTTTG                                    <  SRR4302537.4793502/94‑1 (MQ=60)
                                                           TTCGTTGCGCCGAAGCTTTCTGGTAAAGCAGATTGCATCGGCGCATTTGCCGTGACTGGCGGGCTGGAAGAGGACGCACTGGCTGATGCCTTTG                                    >  SRR4302537.6193669/1‑94 (MQ=60)
                                                            TCGTTGCGCCGAAGCTTTCTGGTAAAGCAGATTACATCGGCGCATTTGCCGTGACTGGCGGGCTGGAAGAGGACGCACTGGCTGATGCCTTTGA                                   <  SRR4302537.4015525/94‑1 (MQ=60)
                                                             CGTTGCGCCGAAGCTTTCTGGTAAAGCAGATTGCATCGGCGCATTTGCCGTGACTGGCGGGCTGGAAGAGGACGCACTGGCTGATGCCTTTGAA                                  <  SRR4302537.555526/94‑1 (MQ=60)
                                                              GTTGCGCCGAAGCTTTCTGGTAAAGCAGATTGCATCGGCGCATTTGCCGTGACTGGCGGGCTGGAAGAGGACGCACTGGCTGATGCCTTTGAAG                                 >  SRR4302537.1994972/1‑94 (MQ=60)
                                                                TGCGCCGAAGCTTTCTGGTAAAGCAGATTGCATCGGCGCATTTGCCGTGACTGGCGGGCTGGAAGAGGACGCACTGGCTGATGCCTTTGAAGCG                               <  SRR4302537.4135520/94‑1 (MQ=60)
                                                                TGCGCCGAAGCTTTCTGGTAAAGCAGATTGCATCGGCGCATTTGCCGTGACTGGCGGGCTGGAAGAGGACGCACTGGCTGATGCCTTTGAAGCG                               <  SRR4302537.1395515/94‑1 (MQ=60)
                                                                TGCGCCGAAGCTTTCTGGTAAAGCAGATTGCATCGGCGCATTTGCCGTGACTGGCGGGCTGGAAGAGGACGCACTGGCTGATGCCTTTGAAGCG                               >  SRR4302537.4436857/1‑94 (MQ=60)
                                                                TGCGCCGAAGCTTTCTGGTAAAGCAGATTGCATCGGCGCATTTGCCGTGACTGGCGGGCTGGAAGAGGACGCACTGGCTGATGCCTTTGAAGCG                               <  SRR4302537.407312/94‑1 (MQ=60)
                                                                   GCCGAAGCTTTCTGGTAAAGCAGATTGCATCGGCGCATTTGCCGTGACTGGCGGGCTGGAAGAGGACGCACTGGCTGATGCCTTTGAAGCGCAG                            <  SRR4302537.2411698/94‑1 (MQ=60)
                                                                   GCCGAAGCTTTCTGGTAAAGCAGATTGCATCGGCGCATTTGCCGTGACTGGCGGGCTGGAAGAGGACGCACTGGCTGATGCCTTTGAAGCGCAG                            <  SRR4302537.2747102/94‑1 (MQ=60)
                                                                   GCCGAAGCTTTCTGGTAAAGCAGATTGCATCGGCGCATTTGCCGTGACTGGCGGGCTGGAAGAGGACGCACTGGCTGATGCCTTTGAAGCGCAG                            <  SRR4302537.3036697/94‑1 (MQ=60)
                                                                    CCGAAGCTTTCTGGTAAAGCAGATTGCATCGGCGCATTTGCCGTGACTGGCGGGCTGGAAGAGGACGCACTGGCTGATGCCTTTGAAGCGCAGC                           >  SRR4302537.996244/1‑94 (MQ=60)
                                                                     CGAAGCTTTCTGGTAAAGCAGATTGCATCGGCGCATTTGCCGTGACTGGCGGGCTGGAAGAGGACGCACTGGCTGATGCCTTTGAAGCGCAGCA                          <  SRR4302537.70877/94‑1 (MQ=60)
                                                                     CGAAGCTTTCTGGTAAAGCAGATTGCATCGGCGCATTTGCCGTGACTGGCGGGCTGGAAGAGGACGCACTGGCTGATGCCTTTGAAGCGCAGCA                          <  SRR4302537.5747294/94‑1 (MQ=60)
                                                                      GAAGCTTTCTGGTAAAGCAGATTGCATCGGCGCATTTGCCGTGACTGGCGGGCTGGAAGAGGACGCACTGGCTGATGCCTTTGAAGCGCAGCAC                         >  SRR4302537.3615098/1‑94 (MQ=60)
                                                                      GAAGCTTTCTGGTAAAGCAGATTGCATCGGCGCATTTGCCGTGACTGGCGGGCTGGAAGAGGACGCACTGGCTGATGCCTTTGAAGCGCAGCAC                         >  SRR4302537.372423/1‑94 (MQ=60)
                                                                       AAGCTTTCTGGTAAAGCAGATTGCATCGGCGCATTTGCCGTGACTGGCGGGCTGGAAGAGGACGCACTGGCTGATGCCTTTGAAGCGCAGCACG                        <  SRR4302537.3568908/94‑1 (MQ=60)
                                                                       AAGCTTTCTGGTAAAGCAGATTGCATCGGCGCATTTGCCGTGACTGGCGGGCTGGAAGAGGACGCACTGGCTGATGCCTTTGAAGCGCAGCACG                        >  SRR4302537.1525199/1‑94 (MQ=60)
                                                                       AAGCTTTCTGGTAAAGCAGATTGCATCGGCGCATTTGCCGTGACTGGCGGGCTGGAAGAGGACGCACTGGCTGATGCCTTTGAAGCGCAGCACG                        >  SRR4302537.3295434/1‑94 (MQ=60)
                                                                       AAGCTTTCTGGTAAAGCAGATTGCATCGGCGCATTTGCCGTGACTAGCGGGCTGGAAGAGGACGCACTGGCTGATGCCTTTGAAGCGCAGCACG                        >  SRR4302537.4417913/1‑94 (MQ=60)
                                                                         GCTTTCTGGTAAAGCAGATTGCATCGGCGCATTTGCCGTGACTGGCGGGCTGGAAGAGGACGCACTGGCTGATGCCTTTGAAGCGCAGCACGAT                      >  SRR4302537.4154933/1‑94 (MQ=60)
                                                                         GCTTTCTGGTAAAGCAGATTGCATCGGCGCATTTGCCGTGACTGGCGGGCTGGAAGAGGACGCACTGGCTGATGCCTTTGAAGCGCAGCACGAT                      <  SRR4302537.1215714/94‑1 (MQ=60)
                                                                         GCTTTCTGGTAAAGCAGATTGCATCGGCGCATTTGCCGTGACTGGCGGGCTGGAAGAGGACGCACTGGCTGATGCCTTTGAAGCGCAGCACGAT                      >  SRR4302537.3458870/1‑94 (MQ=60)
                                                                          CTTTCTGGTAAAGCAGATTGCATCGGCGCATTTGCCGTGACTGGCGGGCTGGAAGAGGACGCACTGGCTGATGCCTTTGAAGCGCAGCACGATG                     >  SRR4302537.2222771/1‑94 (MQ=60)
                                                                          CTTTCTGGTAAAGCAGATTGCATCGGCGCATTTGCCGTGACTGGCGGGCTGGAAGAGGACGCACTGGCTGATGCCTTTGAAGCGCAGCACGATG                     <  SRR4302537.274778/94‑1 (MQ=60)
                                                                          CTTTCTGGTAAAGCAGATTGCATCGGCGCATTTGCCGTGACTGGCGGGCTGGAAGAGGACGCACTGGCTGATGCCTTTGAAGCGCAGCACGATG                     >  SRR4302537.3711698/1‑94 (MQ=60)
                                                                           TTTCTGGTAAAGCAGATTGCATCGGCGCATTTGCCGTGACTGGCGGGCTGGAAGAGGACGCACTGGCTGATGCCTTTGAAGCGCAGCACGATGA                    >  SRR4302537.3999426/1‑94 (MQ=60)
                                                                           TTTCTGGTAAAGCAGATTGCATCGGCGCATTTGCCGTGACTGGCGGGCTGGAAGAGGACGCACTGGCTGATGCCTTTGAAGCGCAGCACGATGA                    >  SRR4302537.815817/1‑94 (MQ=60)
                                                                            TTCTGGTAAAGCAGATTGCATCGGCGCATTTGCCGTGACTGGCGGGCTGGAAGAGGACGCACTGGCTGATGCCTTTGAAGCGCAGCACGATGAT                   <  SRR4302537.4120522/94‑1 (MQ=60)
                                                                              CTGGTAAAGCAGATTGCATCGGCGCATTTGCCGTGCCTGGCGGGCTGGAAGAGGACGCACTGGCTGATGCCTTTGAAGCGCAGCACGATGATTA                 <  SRR4302537.6266338/94‑1 (MQ=60)
                                                                              CTGGTAAAGCAGATTGCATCGGCGCATTTGCCGTGACTGGCGGGCTGGAAGAGGACGCACTGGCTGATGCCTTTGAAGCGCAGCACGATGATTA                 <  SRR4302537.4400363/94‑1 (MQ=60)
                                                                              CTGGTAAAGCAGATTGCATCGGCGCATTTGCCGTGACTGGCGGGCTGGAAGAGGACGCACTGGCTGATGCCTTTGAAGCGCAGCACGATGATTA                 >  SRR4302537.4716042/1‑94 (MQ=60)
                                                                              CTGGTAAAGCAGATTGCATCGGCGCATTTGCCGTGACTGGCGGGCTGGAAGAGGACGCACTGGCTGATGCCTTTGAAGCGCAGCACGATGATTA                 <  SRR4302537.2183319/94‑1 (MQ=60)
                                                                                 GTAAAGCAGATTGCATCGGCGCATTTGCCGTGACTGGCGGGCTGGAAGAGGACGCACTGGCTGATGCCTTTGAAGCGCAGCACGATGATTACAA              <  SRR4302537.5548091/94‑1 (MQ=60)
                                                                                   AAAGCAGATTGCATCGTCGCATTTGCCGTGACTGGCGGGCTGGAAGAGGACGCACTGGCTGATGCCTTTGAAGCGCAGCACGATGATTACAACA            <  SRR4302537.5821420/94‑1 (MQ=60)
                                                                                   AAAGCAGATTGCATCGGCGCATTTGCCGTGACTGGCGGGCTGGAAGAGGACGCACTGGCTGATGCCTTTGAAGCGCAGCACGATGATTACAACA            <  SRR4302537.5890276/94‑1 (MQ=60)
                                                                                   AAAGCAGATTGCATCGGCGCATTTGCCGTGACTGGCGGGCTGGAAGAGGACGCACTGGCTGATGCCTTTGAAGCGCAGCACGATGATTACAACA            >  SRR4302537.1862561/1‑94 (MQ=60)
                                                                                   AAAGCAGATTGCATCGGCGCATTTGCCGTGACTGGCGGGCTGGAAGAGGACGCACTGGCTGATGCCTTTGAAGCGCAGCACGATGATTACAACA            <  SRR4302537.2398940/94‑1 (MQ=60)
                                                                                       CAGATTGCATCGGCGCATTTGCCGTGACTGGCGGGCTGGAAGAGGACGCACTGGCTGATGCCTTTGAAGCGCAGCACGATGATTACAACAAAAT        <  SRR4302537.482048/94‑1 (MQ=60)
                                                                                       CAGATTGCATCGGCGCATTTGCCGTGACTGGCGGGCTGGAAGAGGACGCACTGGCTGATGCCTTTGAAGCGCAGCACGATGATTACAACAAAAT        <  SRR4302537.2064119/94‑1 (MQ=60)
                                                                                        AGATTGCATCGGCGCATTTGCCGTGACTGGCGGGCTGGAAGAGGACGCACTGGCTGATGCCTTTGAAGCGCAGCACGATGATTACAACAAAATC       >  SRR4302537.2666796/1‑94 (MQ=60)
                                                                                        AGATTGCATCGGCGCATTTGCCGTGACTGGCGGGCTGGAAGAGGACGCACTGGCTGATGCCTTTGAAGCGCAGCACGATGATTACAACAAAATC       >  SRR4302537.5470359/1‑94 (MQ=60)
                                                                                        AGATTGCATCGGCGCATTTGCCGTGACTGGCGGGCTGGAAGAGGACGCACTGGCTGATGCCTTTGAAGCGCAGCACGATGATTACAACAAAATA       >  SRR4302537.4389658/1‑94 (MQ=60)
                                                                                          ATTGCATCGGCGCATTTGCCGTGACTGGCGGGCTGGAAGAGGACGCACTGGCTGATGCCTTTGAAGCGCAGCACGATGATTACAACAAAATCAT     >  SRR4302537.4755450/1‑94 (MQ=60)
                                                                                          ATTGCATCGGCGCATTTGCCGTGACTGGCGGGCTGGAAGAGGACGCACTGGCTGATGCCTTTGAAGCGCAGCACGATGATTACAACAAAATCAT     <  SRR4302537.5725314/94‑1 (MQ=60)
                                                                                          ATTGCATCGGCGCATTTGCCGTGACTGGCGGGCTGGAAGAGGACGCACTGGCTGATGCCTTTGAAGCGCAGCACGATGATTACAACAAAATCAT     >  SRR4302537.2283295/1‑94 (MQ=60)
                                                                                           TTGCATCGGCGCATTTGCCGTGACTGGCGGGCTGGAAGAGGACGCACTGGCTGATGCCTTTGAAGCGCAGCACGATGATTACAACAAAATCATG    >  SRR4302537.4588668/1‑94 (MQ=60)
                                                                                           TTGCATCGGCGCATTTGCCGTGACTGGCGGGCTGGAAGAGGACGCACTGGCTGATGCCTTTGAAGCGCAGCACGATGATTACAACAAAATCATG    <  SRR4302537.6307051/94‑1 (MQ=60)
                                                                                           TTGCATCGGCGCATTTGCCGTGACTGGCGGGCTGGAAGAGGACGCACTGGCTGATGCCTTTGAAGCGCAGCACGATGATTACAACAAAATCATG    <  SRR4302537.4585590/94‑1 (MQ=60)
                                                                                           TTGCATCGGCGCATTTGCCGTGACTGGCGGGCTGGAAGAGGACGCACTGGCTGATGCCTTTGAAGCGCAGCACGATGATTACAACAAAATCATG    <  SRR4302537.3398222/94‑1 (MQ=60)
                                                                                            TGCATCGGCGCATTTGCCGTGACTGGCGGGCTGGAAGAGGACGCACTGGCTGATGCCTTTGAAGCGCAGCACGATGATTACAACAAAATCATGG   >  SRR4302537.269769/1‑94 (MQ=60)
                                                                                             TCATCGGCGCATTTGCCGTGACTGGCGGGCTGGAAGAGGACGCACTGGCTGATGCCTTTGAAGCGCAGCACGATGATTACAACAAAATCATGGT  <  SRR4302537.5968175/94‑1 (MQ=60)
                                                                                             GCATCGGCGCATTTGCCGTGACTGGCGGGCTGGAAGAGGACGCACTGGCTGATGCCTTTGAAGCGCAGCACGATGATTACAACAAAATCATGGT  <  SRR4302537.5317504/94‑1 (MQ=60)
                                                                                             |                                                                                             
GCCACCATCTGCGTCAACAGACCGAAAAAACAGGCTTCGCTAACTACTGTCTCGCTGACTTCGTTGCGCCGAAGCTTTCTGGTAAAGCAGATTACATCGGCGCATTTGCCGTGACTGGCGGGCTGGAAGAGGACGCACTGGCTGATGCCTTTGAAGCGCAGCACGATGATTACAACAAAATCATGGT  >  NC_000913/4224483‑4224669

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑