Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	882	254.4	12957588	98.0%	12698436	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,233,673	C→T	G63G (GGC→GGT)	yjbE →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,233,673	0	C	T	96.1%	772.7 / 8.2	229	G63G (GGC→GGT)	yjbE	hypothetical protein
Reads supporting (aligned to +/- strand): ref base C (2/7); new base T (86/134); total (88/141)
Fisher's exact test for biased strand distribution p-value = 4.88e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.78e-01

Only 100 of 243 total aligned reads displayed.

GCAACGTCGGTTTCGGCGGGGAGTTCCTCCGCGACCAGCGTCAGCACCGTAAGCTCGGCGGTGGGTGTCGCGCTTGCGGCAA‑CCGGTGGCGGTGATGGTTCTAATACCGGGACCACCACAACCACGACCACCAGTACCCAGTAATAAAGTATGTATCCCCAAAATAATTCGAGTCATTG  >  NC_000913/4233584‑4233762

gcaACGTCGGTTTCGGCGGGGAGTTCCTCCGCGACCAGCGTCAGCACCGTAAGCTCGGCGGTGGGTGTCGCGCTTGCGGCAA‑CCGGtggtggg                                                                                        >  2:4270046/1‑92 (MQ=255)

  aaCGTCGGTTTCGGCGGGGAGTTCCTCCGCGACCAGCGTCAGCACCGTAAGCTCGGCGGTGGGTGTCGCGCTTGCGGCAA‑CCGGTGGTGGTGa                                                                                      >  1:4986433/1‑93 (MQ=255)

  aaCGTCGGTTTCGGCGGGGAGTTCCTCCGCGACCAGCGTCAGCACCGTAAGCTCGGCGGTGGGTGTCGCGCTTGCGGCAA‑CCGGTGGTGGTGa                                                                                      <  2:3751671/93‑1 (MQ=255)

   aCGTCGGTTTCGGCGGGGAGTTCCTCCGCGACCAGCGTCAGCACCGTAAGCTCGGCGGTGGGTGTCGCGCTTGCGGCAA‑CCGGTGGTGGTGAt                                                                                     <  1:3601337/93‑1 (MQ=255)

   aCGTCGGTTTCGGCGGGGAGTTCCTCCGCGACCAGCGTCAGCACCGTAAGCTCGGCGGTGGGTGTCGCGCTTGCGGCAA‑CCGGTGGTGGTGAt                                                                                     <  2:390427/93‑1 (MQ=255)

     gTCGGTTTCGGCGGGGAGTTCCTCCGCGACCAGCGTCAGCACCGTAAGCTCGGCGGTGGGTGTCGCGCTTGCGGCAA‑CCGGTGGTGGTGATgg                                                                                   <  2:2830288/93‑1 (MQ=255)

     gTCGGTTTCGGCGGGGAGTTCCTCCGCGACCAGCGTCAGCACCGTAAGCTCGGCGGTGGGTGTCGCGCTTGCGGCAA‑CCGGTGGTGGTGATgg                                                                                   <  2:5332029/93‑1 (MQ=255)

      tCGGTTTCGGCGGGGAGTTCCTCCGCGACCAGCGTCAGCACCGTAAGCTCGGCGGTGGGTGTCGCGCTTGCGGCAA‑CCGGTGGTGGTGATGGt                                                                                  >  2:2902906/1‑93 (MQ=255)

      tCGGTTTCGGCGGGGAGTTCCTCCGCGACCAGCGTCAGCACCGTAAGCTCGGCGGTGGGTGTCGCGCTTGCGGCAA‑CCGGTGGTGGTGATGGt                                                                                  >  1:5721404/1‑93 (MQ=255)

        ggTTTCGGCGGGGAGTTCCTCCGCGACCAGCGTCAGCACCGTAAGCTCGGCGGTGGGTGTCGCGCTTGCGGCAA‑CCGGTGGTGGTGATGGTTc                                                                                <  1:423781/93‑1 (MQ=255)

        ggTTTCGGCGGGGAGTTCCTCCGCGACCAGCGTCAGCACCGTAAGCTCGGCGGTGGGTGTCGCGCTTGCGGCAA‑CCGGTGGTGGTGATGGTTc                                                                                <  2:601167/93‑1 (MQ=255)

        ggTTTCGGCGGGGAGTTCCTCCGCGACCAGCGTCAGCACCGTAAGCTCGGCGGTGGGTGTCGCGCTTGCGGCAA‑CCGGTGGTGGTGATGGTTc                                                                                >  2:3430248/1‑93 (MQ=255)

         gTTTCGGCGGGGAGTTCCTCCGCGACCAGCGTCAGCACCGTAAGCTCGGCGGTGGGTGTCGCGCTTGCGGCAA‑CCGGTGGTGGTGATGGTTCt                                                                               >  1:3245336/1‑93 (MQ=255)

          tttCGGCGGGGAGTTCCTCCGCGACCAGCGTCAGCACCGTAAGCTCGGCGGTGGGTGTCGCGCTTGCGGCAA‑CCGGTGGCGGTGATGGTTCTa                                                                              >  1:3847852/1‑93 (MQ=255)

          tttCGGCGGGGAGTTCCTCCGCGACCAGCGTCAGCACCGTAAGCTCGGCGGGGGGTGTCGCGCTTGCGGCAA‑CCGGTGGTGGGGATGGTTCTa                                                                              >  1:5278720/1‑93 (MQ=255)

           ttCGGCGGGGAGTTCCTCCGCGACCAGCGTCAGCACCGTAAGCTCGGCGGTGGGTGTCGCGCTTGCGGCAA‑CCGGTGGTGGTGATGGTTCTaa                                                                             <  2:5068178/93‑1 (MQ=255)

            tCGGCGGGGAGTTCCTCCGCGACCAGCGTCAGCACCGTAAGCTCGGCGGTGGGTGTCGCGCTTGCGGCAA‑CCGGTGGTGGTGATGGGTCTAAt                                                                            >  2:3616640/1‑93 (MQ=255)

              ggcggGGAGTTCCTCCGCGACCAGCGTCAGCACCGTAAGCTCGGCGGTGGGTGTCGCGCTTGCGGCAA‑CCGGTGGTGGTGATGGTTCTAATAc                                                                          <  1:2975285/93‑1 (MQ=255)

              ggcggGGAGTTCCTCCGCGACCAGCGTCAGCACCGTAAGCTCGGCGGTGGGTGTCGCGCTTGCGGCAA‑CCGGTGGTGGTGATGGTTCTAATAc                                                                          <  2:2437373/93‑1 (MQ=255)

              ggcggGGAGTTCCTCCGCGACCAGCGTCAGCACCGTAAGCTCGGCGGTGGGTGTCGCGCTTGCGGCAA‑CCGGTGGTGGTGATGGTTCTAATAc                                                                          >  2:3110149/1‑93 (MQ=255)

                cggGGAGTTCCTCCGCGACCAGCGTCAGCACCGTAAGCTCGGCGGTGGGTGTCGCGCTTGCGGCAA‑CCGGTGGTGGTGATGGTTCTAATACCg                                                                        <  2:3323537/93‑1 (MQ=255)

                cggGGAGTGCCTCCGCGACCAGCGTCAGCACCGTAAGCTCGGCGGTGGGTGTCGCGCTTGCGGCAA‑CCGGTGGTGGTGATGGTTCTAATACCg                                                                        <  1:2338619/93‑1 (MQ=255)

                 gggtAGTGCATCCGCGACCAGCGTCAGCACCGTAAGCTCGGCGGTGGGTGTCGCGCTTGCGGCAA‑CCGGTGGTGGTGATGGTTCTAATACCgg                                                                       <  1:2861619/93‑1 (MQ=255)

                 ggggAGTTCCTCCGCGACCAGCGTCAGCACCGTAAGCTCGGCGGTGGGTGTCGCGCTTGCGGCAA‑CCGGTGGTGGTGATGGTTCTAATACCgg                                                                       >  2:2183905/1‑93 (MQ=255)

                 ggggAGTTCCTCCGCGACCAGCGTCAGCACCGTAAGCTCGGCGGTGGGTGTCGCGCTTGCGGCAA‑CCGGTGGTGGTGATGGTTCTAATACCgg                                                                       <  2:3131568/93‑1 (MQ=255)

                  gggAGTTCCTCCGCGACCAGCGTCAGCACCGTAAGCTCGGCGGTGGGTGTCGCGCTTGCGGCAA‑CCGGTGGTGGTGATGGTTCTAATACCggg                                                                      >  1:2222589/1‑93 (MQ=255)

                   ggAGTTCCTCCGCGACCAGCGTCAGCACCGTAAGCTCGGCGGTGGGTGTCGCGCTTGCGGCAA‑CCGGTGGTGGTGATGGTTCTAATACCGGGa                                                                     >  1:1892794/1‑93 (MQ=255)

                      gTTCCTCCGCGACCAGCGTCAGCACCGTAAGCTCGGCGGTGGGTGTCGCGCTTGCGGCAA‑CCGGTGGTGGTGATGGTTCTAATACCGGGacca                                                                  >  2:3686431/1‑93 (MQ=255)

                      gTTCCTCCGCGACCAGCGTCAGCACCGTAAGCTCGGCGGTGGGTGTCGCGCTTGCGGCAA‑CCGGTGGTGGTGATGGTTATAATACCGGGacca                                                                  >  2:5302050/1‑93 (MQ=255)

                       ttCCTCCGCGACCAGCGTCAGCACCGTAAGCTCGGCGGTGGGTGTCGCGCTTGCGGCAA‑CCGGTGGTGGTGATGGTTCTAATACCGGGaccac                                                                 >  1:6014287/1‑93 (MQ=255)

                       ttCCTCCGCGACCAGCGTCAGCACCGTAAGCTCGGCGGTGGGTGTCGCGCTTGCGGCAA‑CCGGTGGCGGTGATGGTTCTAATACCGGGaccac                                                                 <  2:4753051/93‑1 (MQ=255)

                        tcctccGCGACCAGCGTCAGCACCGTAAGCTCGGCGGTGGGTGTCGCGCTTGCGGCAA‑CCGGTGGTGGTGATGGTTCTAATACCGGGaccacc                                                                >  1:631212/1‑93 (MQ=255)

                         cctccGCGACCAGCGTCAGCACCGTAAGCTCGGCGGTGGGTGTCGCGCTTGCGGCAA‑CCGGTGGTGGTGATGGTTCTAATACCGGGAccacca                                                               <  1:452708/93‑1 (MQ=255)

                          ctccGCGACCAGCGTCAGCACCGTAAGCTCGGCGGTGGGTGTCGCGCTTGCGGCAA‑CCGGTGGTGGTGATGGTTCTAATACCGGGACcaccac                                                              <  2:4980189/93‑1 (MQ=255)

                             cgcgACCAGCGTCAGCACCGTAAGCTCGGCGGTGGGTGTCGCGCTTGCGGCAA‑CCGGTGGTGGTGATGGTTCTAATACCGGGACCACCACAAc                                                           >  1:5094840/1‑93 (MQ=255)

                               cgACCAGCGTCAGCACCGTAAGCTCGGCGGTGGGTGTCGCGCTTGCGGCAA‑CCGGTGGTGGTGATGGTTCTAATACCGGGACCACCACAACCa                                                         >  2:4608430/1‑93 (MQ=255)

                               cgACCAGCGTCAGCACCGTAAGCTCGGCGGTGGGTGTCGCGCTTGCGGCAA‑CCGGTGGTGGTGATGGTTCTAATACCGGGACCACCACAACCa                                                         <  2:5648527/93‑1 (MQ=255)

                                gACCAGCGTCAGCACCGTAAGCTCGGCGGTGGGTGTCGCGCTTGCGGCAA‑CCGGTGGTGGTGATGGTTCTAATACCGGGACCACCACAACCAc                                                        <  1:3161714/93‑1 (MQ=255)

                                gACCAGCGTCAGCACCGTAAGCTCGGCGGTGGGTGTCGCGCTTGCGGCAA‑CCGGTGGTGGTGATGGTTCTAATACCGGGACCACCACAACCAc                                                        >  2:4324406/1‑93 (MQ=255)

                                gACCAGCGTCAGCACCGTAAGCTCGGCGGTGGGTGTCGCGCTTGCGGCAA‑CCGGTGGTGGTGATGGTTCTAATACCGGGACCACCACAACCAc                                                        >  2:1649243/1‑93 (MQ=255)

                                 aCCAGCGTCAGCACCGTAAGCTCGGCGGTGGGTGTCGCGCTTGCGGCAA‑CCGGTGGTGGTGATGGTTCTAATACCGGGACCACCACAACCACg                                                       >  2:6431613/1‑93 (MQ=255)

                                 aCCAGCGTCAGCACCGTAAGCTCGGCGGTGGGTGTCGCGCTTGCGGCAA‑CCGGTGGCGGTGATGGTTCTAATACCGGGACCACCACAACCACg                                                       <  1:3678686/93‑1 (MQ=255)

                                   cAGCGTCAGCACCGTAAGCTCGGCGGTGGGTGTCGCGCTTGCGGCAA‑CCGGTGGTGGTGATGGTTCTAATACCGGGACCACCACAACCACGac                                                     <  2:1999837/93‑1 (MQ=255)

                                    aGCGTCAGCACCGTAAGCTCGGCGGTGGGTGTCGCGCTTGCGGCAA‑CCGGTGGTGGTGATGGTTCTAATACCGGGACCACCACAACCACGacc                                                    <  1:1598764/93‑1 (MQ=255)

                                     gCGTCAGCACCGTAAGCTCGGCGGTGGGTGTCGCGCTTGCGGCAA‑CCGGTGGTGGTGATGGTTCTAATACCGGGACCACCACAACCACGacca                                                   <  1:57769/93‑1 (MQ=255)

                                     gCGTCAGCACCGTAAGCTCGGCGGTGGGTGTCGCGCTTGCGGCAA‑CCGGTGGTGGTGATGGTTCTAATACCGGGACCACCACAACCACGacca                                                   >  2:5797872/1‑93 (MQ=255)

                                     gCGTCAGCACCGTAAGCTCGGCGGTGGGTGTCGCGCTTGCGGCAA‑CCGGTGGTGGTGATGGTTCTAATACCGGGACCACCACAACCACGacca                                                   >  2:4442038/1‑93 (MQ=255)

                                          aGCACCGTAAGCTCGGCGGTGGGTGTCGCGCTTGCGGCAA‑CCGGTGGTGGTGATGGTTCTAATACCGGGACCACCACAACCACGACCACCAGt                                              >  1:2676177/1‑93 (MQ=255)

                                           gCACCGTAAGCTCGGCGGTGGGTGTCGCGCTTGCGGCAA‑CCGGTGGTGGTGATGGTTCTAATACCGGGACCACCACAACCACGACCACCAGTa                                             <  2:5559443/93‑1 (MQ=255)

                                            cACCGTAAGCTCGGCGGTGGGTGTCGCGCTTGCGGCAA‑CCGGTGGTGGTGATGGTTCTAATACCGGGACCACCACAACCACGACCACCAGTAc                                            <  1:1425218/93‑1 (MQ=255)

                                            cACCGTAAGCTCGGCGGTGGGTGTCGCGCTTGCGGCAA‑CCGGTGGTGGTGATGGTTCTAATACCGGGACCACCACAACCACGACCACCAGTAc                                            >  2:1559196/1‑93 (MQ=255)

                                             aCCGTAAGCTCGGCGGTGGGTGTCGCGCTTGCGGCAA‑CCGGTGGTGGTGATGGTTCTAATACCGGGACCACCACAACCACGACCACCAGTAcc                                           <  1:791799/93‑1 (MQ=255)

                                              ccGTAAGCTCGGCGGTGGGTGTCGCGCTTGCGGCAA‑CCGGTGGTGGTGATGGTTCTAATACCGGGACCACCACAACCACGACCACCAGTAccc                                          >  1:3667261/1‑93 (MQ=255)

                                               cGTAAGCTCGGCGGTGGGTGTCGCGCTTGCGGCAA‑CCGGTGGTGGTGATGGTTCTAATCCCGGGACCACCACAACCACGACCACCAGTACCCa                                         <  1:687066/93‑1 (MQ=255)

                                               cGTAAGCTCGGCGGTGGGTGTCGCGCTTGCGGCAA‑CCGGTGGTGGTGATGGTTCTAATACCGGGACCACCACAACCACGACCACCAGTACCCa                                         <  1:6221018/93‑1 (MQ=255)

                                               cGTAAGCTCGGCGGTGGGTGTCGCGCTTGCGGCAA‑CCGGTGGTGGTGATGGTTCTAATACCGGGACCACCACAACCACGACCACCAGTACCCa                                         <  1:6082346/93‑1 (MQ=255)

                                               cGTAAGCTCGGCGGTGGGTGTCGCGCTTGCGGCAA‑CCGGTGGTGGTGATGGTTCTAATACCGGGACCACCACAACCACGACCACCAGTACCCa                                         <  1:4433713/93‑1 (MQ=255)

                                                gTAAGCTCGGCGGTGGGTGTCGCGCTTGCGGCAA‑CCGGTGGTGGTGATGGTTCTAATACCGGGACCACCACAACCACGACCACCAGTACCCAg                                        <  2:2139686/93‑1 (MQ=255)

                                                  aaGCTCGGCGGTGGGTGTCGCGCTTGCGGCAA‑CCGGTGGTGGTGATGGTTCTAATACCGGGACCACCACAACCACGACCACCAGTACCCAGta                                      <  1:2526808/93‑1 (MQ=255)

                                                   aGCTCGGCGGTGGGTGTCGCGCTTGCGGCAA‑CCGGTGGTGGTGATGGTTCTAATACCGGGACCACCACAACCACGACCACCAGTACCCAGtaa                                     <  1:5310800/93‑1 (MQ=255)

                                                    gCTCGGCGGTGGGTGTCGCGCTTGCGGCAA‑CCGGTGGTGGTGATGGTTCTAATACCGGGACCACCACAACCACGACCACCAGTACCCAGtaat                                    <  2:1397835/93‑1 (MQ=255)

                                                     cTCGGCGGTGGGTGTCGCGCTTGCGGCAA‑CCGGTGGTGGTGATGGTTCTAATACCGGGACCACCACAACCACGACCACCAGTACCCAGtaata                                   <  2:544393/93‑1 (MQ=255)

                                                     cTCGGCGGTGGGTGTCGCGCTTGCGGCAA‑CCGGTGGTGCTGATGGTTCTAATACCGGGACCACCACAACCACGACCACCAGTACCCAGtaata                                   <  2:931853/93‑1 (MQ=255)

                                                      tCGGCGGTGGGTGTCGCGCTTGCGGCAA‑CCGGTGGTGGTGATGGTTCTAATACCGGGACCACCACAACCACGACCACCAGTACCCAGtaataa                                  >  2:3076987/1‑93 (MQ=255)

                                                       cggcggTGGGTGTCGCGCTTGCGGCAA‑CCGGTGGTGGTGATGGTTCTAATACCGGGACCACCACAACCACGACCACCAGTACCCAGTAATaaa                                 <  2:6148029/93‑1 (MQ=255)

                                                        ggcggTGGGTGTCGCGCTTGCGGCAA‑CCGGTGGTGGTGATGGTTCTAATACCGGGACCACCACAACCACGACCACCAGTACCCAGTAATAAAg                                <  1:4537943/93‑1 (MQ=255)

                                                         gcggTGGGTGTCGCGCTTGCGGCAA‑CCGGTGGTGGTGATGGTTCTAATACCGGGACCACCACAACCACGACCACCAGTACCCAGTAATAAAgt                               >  1:3377862/1‑93 (MQ=255)

                                                         gcggTGGGTGTCGCGCTTGCGGCAA‑CCGGTGGTGGTGATGGTTCTAATACCGGGACCACCACAACCACGACCACCAGTACCCAGTAATAAAgt                               <  2:2331450/93‑1 (MQ=255)

                                                         gcggTGGGTGTCGCGCTTGCGGCAA‑CCGGTGGTGGTGATGGTTCTAATACCGGGACCACCACAACCACGACCACCAGTACCCAGTAATAAAgt                               <  1:2713558/93‑1 (MQ=255)

                                                         gcggTGGGTGTCGCGCTTGCGGCAA‑CCGGTGGTGGTGATGGTTCTAATACAGGGACCACCACAACACCGACTACCAGTACCCAGTAATAAAgt                               >  1:5560157/1‑93 (MQ=255)

                                                          cggTGGGTGTCGCGCTTGCGGCAA‑CCGGTGGTGGTGATGGTTCTAATACCGGGACCACCACAACCACGACCACCAGTACCCAGTAATAAAgta                              <  2:39853/93‑1 (MQ=255)

                                                           ggtgggtgGCGCGATTG‑GGCAAGCCGTTGTTGGTGATGGTTATAATACCGGGACCACCACAACCACGACCACCAGTACCCAGTAATAAAgtat                             <  1:877448/93‑1 (MQ=255)

                                                            gtgggtgTCGCGCTTGCGGCAA‑CCGGTGGTGGTGATGGTTCTAATACCGGGACCACCACAACCACGACCACCAGTACCCAGTAATAAAgtatg                            <  1:970649/93‑1 (MQ=255)

                                                             tggttgTCGCGCTTGCGGCAA‑CCGGTGGCGGTGATGGTTCTAATACCGGGACCACCACAACCACGACCACCAGTACCCAGTAATAAAgtatgt                           <  2:4845141/93‑1 (MQ=255)

                                                             tgggtgTCGCGCTTGCGGCAA‑CCGGTGGTGGTGATGGTTCTAATACCGGGACCACCACAACCACGACCACCAGTACCCAGTAATAAAgtatgt                           <  2:5241680/93‑1 (MQ=255)

                                                               ggtgTCGCGCTTGCGGCAA‑CCGGTGGTGGTGATGGTTCTAATACCGGGACCACCACAACCACGACCACCAGTACCCAGTAATAAAgtatgtat                         <  1:3120619/93‑1 (MQ=255)

                                                               ggtgTCGCGCTTGCGGCAA‑CCGGTGGTGGTGATGGTTCTAATACCGGGACCACCACAACCACGACCACCAGTACCCAGTAATAAAgtatgtat                         >  1:291829/1‑93 (MQ=255)

                                                               ggtgTCGCGCTTGCGGCAA‑CCGGTGGTGGTGATGGTTCTAATACCGGGACCACCACAACCACGACCACCAGTACCCAGTAATAAAgtatgtat                         <  1:4198375/93‑1 (MQ=255)

                                                                gtgtCGCGCTTGCGGCAA‑CCGGTGGTGGTGATGGTTCTAATACCGGGACCACCACAACCACGACCACCAGTACCCAGTAATAAAGTATGTATc                        <  2:3453815/93‑1 (MQ=255)

                                                                     gcgcTTGCGGCAA‑CCGGTGGTGGTGATGGTTCTAATACCGGGACCACCACAACCACGACCACCAGTACCCAGTAATAAAGTATGTATCCCCaa                   >  2:2628609/1‑93 (MQ=255)

                                                                      cgcTTGCGGCAA‑CCGGTGGTGGTGATGGTTCTAATACCGGGACCACCACAACCACGACCACCAGTACCCAGTAATAAAGTATGTATCCCCaaa                  >  2:492364/1‑93 (MQ=255)

                                                                       gcTTGCGGCAA‑CCGGTGGTGGTGATGGTTCTAATACCGGGACCACCACAACCACGACCACCAGTACCCAGTAATAAAGTATGTATCCCCaaaa                 <  2:6280388/93‑1 (MQ=255)

                                                                        cTTGCGGCAA‑CCGGTGGTGGTGATGGTTCTAATACCGGGACCACCACCACCACGACCACCAGTACCCAGTAATAAAGTATGTATCCCCAAaat                >  2:541589/1‑93 (MQ=255)

                                                                        cTTGCGGCAA‑CCGGTGGTGGTGATGGTTCTAATACCGGGACCACCACAACCACGACCACCAGTACCCAGTAATAAAGTATGTATCCCCAAaat                >  1:4095860/1‑93 (MQ=255)

                                                                          tGCGGCAA‑CCGGTGGTGGTGATGGTTCTAATACCGGGACCACCACAACCACGACCACCAGTACCCAGTAATAAAGTATGTATCCCCAAaataa              >  2:6058908/1‑93 (MQ=255)

                                                                            cGGCAA‑CCGGTGGTGGTGATGGTTCTAATACCGGGACCACCACAACCACGACCACCAGTACCCAGTAATAAAGTATGTATCCCCAAAATAAtt            <  1:2265947/93‑1 (MQ=255)

                                                                            cGGCAA‑CCGGTGGTGGTGATGGTTCTAATACCGGGACCACCACAACCACGACCACCAGTACCCAGTAATAAAGTATGTATCCCCAAAATAAtt            <  2:5918621/93‑1 (MQ=255)

                                                                             ggCAA‑CCGGTGGTGGTGATGGTTCTAATACCGGGACCACCACAACCACGACCACCAGTACCCAGTAATAAAGTATGTATCCCCAAAATAATTc           <  2:4709027/93‑1 (MQ=255)

                                                                              gCAA‑CCGGTGGTGGTGATGGTTCTAATACCGGGACCACCACAACCACGACCACCAGTACCCAGTAATAAAGTATGTATCCCCAAAATAATTCg          >  2:1235049/1‑93 (MQ=255)

                                                                              gCAA‑CCGGTGGTGGTGATGGTTCTAATACCGGGACCACCACAACCACGACCACCAGTACCCAGTAATAAAGTATGTATCCCCAAAATAATTCg          >  2:2761130/1‑93 (MQ=255)

                                                                               cAA‑CCGGTGGTGGTGATGGTTCTAATACCGGGACCACCACACCCACGACCACCAGTACCCAGTAATAAAGTATGTATCCCCAAAATAATTCGa         <  2:3988629/93‑1 (MQ=255)

                                                                                aa‑CCGGTGGTGGTGATGGTTCTAATACCGGGACCACCACAACCACGACCACCAGTACCCAGTAATAAAGTATGTATCCCCAAAATAATTCGAg        <  2:5391895/93‑1 (MQ=255)

                                                                                aa‑CCGGTGGTGGTGATGGTTCTAATACCGGGACCACCACAACCACGACCACCAGTACCCAGTAATAAAGTATGTATCCCCAAAATAATTCGAg        >  1:1711836/1‑93 (MQ=255)

                                                                                aa‑CCGGTGGTGGTGATGGTTCTAATACCGGGACCACCACAACCACGACCACCAGTACCCAGTAATAAAGTATGTATCCCCAAAATAATTCGAg        >  1:1045094/1‑93 (MQ=255)

                                                                                 a‑CCGGTGGTGGTGATGGTTCTAATACCGGGACCACCACAACCACGACCACCAGTACCCAGTAATAAAGTATGTATCCCCAAAATAATTCGAGt       >  1:6426548/1‑93 (MQ=255)

                                                                                   ccGGTGGTGGTGATGGTTCTAATACCGGGACCACCACAACCACGACCACCAGTACCCAGTAATAAAGTATGTATCCCCAAAATAATTCGAGTc      <  1:1775681/93‑1 (MQ=255)

                                                                                     ggtggtggtGATGGTTCTAATACCGGGACCACCACAACCACGACCACCAGTACCCAGTAATAAAGTATGTATCCCCAAAATAATTCGAGTCAt    <  1:1256427/93‑1 (MQ=255)

                                                                                       tggtggTGATGGTTCTAATACCGGGACCACCACAACCACGACCACCAGTACCCAGTAATAAAGTATGTATCCCCAAAATAATTCGAGTCATTg  >  2:3296003/1‑93 (MQ=255)

                                                                                       tggtggTGATGGTTCTAATACCGGGACCACCACAACCACGACCACCAGTACCCAGTAATAAAGTATGTATCCCCAAAATAATTCGAGTCATTg  <  1:2182410/93‑1 (MQ=255)

                                                                                       tggtggTGATGGTTCTAATACCGGGACCACCACAACCACGACCACCAGTACCCAGTAATAAAGTATGTATCCCCAAAATAATTCGAATCATTg  >  2:4236589/1‑93 (MQ=255)

GCAACGTCGGTTTCGGCGGGGAGTTCCTCCGCGACCAGCGTCAGCACCGTAAGCTCGGCGGTGGGTGTCGCGCTTGCGGCAA‑CCGGTGGCGGTGATGGTTCTAATACCGGGACCACCACAACCACGACCACCAGTACCCAGTAATAAAGTATGTATCCCCAAAATAATTCGAGTCATTG  >  NC_000913/4233584‑4233762

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

GGCAGCAACGTCGGTTTCGGCGGGGAGTTCCTCCGCGACCAGCGTCAGCACCGTAAGCTCGGCGGTGGGTGTCGCGCTTGCGGCAACCGGTGGCGGTGATGGTTCTAATACCGGGACCACCACAACCACGACCACCAGTACCCAGTAATAAAGTATGTATCCCCAAAATAATTCGAGTCATTGCATC  >  NC_000913/4233580‑4233766

GGCAGCAACGTCGGTTTCGGCGGGGAGTTCCTCCGCGACCAGCGTCAGCACCGTAAGCTCGGCGGTGGGTGTCGCGCTTGCGGCAACCGGTGGG                                                                                               >  SRR4302537.1340294/1‑94 (MQ=60)

 GCAGCAACGTCGGTTTCGGCGGGGAGTTCCTCCGCGACCAGCGTCAGCACCGTAAGCTCGGCGGTGGGTGTCGCGCTTGCGGCAACCGGTGGTG                                                                                              <  SRR4302537.983387/94‑1 (MQ=60)

  CAGCAACGTCGGTTTCGGCGGGGAGTTCCTCCGCGACCAGCGTCAGCCCCGTAAGCTCGGCGGTGGGGGTCGCGCTTGGGGCAACCGGTGGGGG                                                                                             >  SRR4302537.585892/1‑94 (MQ=60)

  CAGCAACGTCGGTTTCGGCGGGGAGTTCCTCCGCGACCAGCGTCAGCACCGTAAGCTCGGCGGTGGGTGTCGCGCTTGCGGCAACCGGTGGTGG                                                                                             <  SRR4302537.3816257/94‑1 (MQ=60)

   AGCAACGTCGGTTTCGGCGGGGAGTTCCTCCGCGACCAGCGTCAGCACCGTAAGCTCGGCGGTGGGTGTCGCGCTTGCGGCCACCGGTGGCGGG                                                                                            >  SRR4302537.2979026/1‑94 (MQ=60)

     CAACGTCGGTTTCGGCGGGGAGTTCCTCCGCGACCAGCGTCAGCACCGTAAGCTCGGCGGTGGGTGTCGCGCTTGCGGCAACCGGTGGTGGTGA                                                                                          >  SRR4302537.5020799/1‑94 (MQ=60)

       ACGTCGGTTTCGGCGGGGAGTTCCTCCGCGACCAGCGTCAGCACCGTAAGCTCGGCGGTGGGTGTCGCGCTTGCGGCAACCGGTGGTGGTGATG                                                                                        <  SRR4302537.3626720/94‑1 (MQ=60)

         GTCGGTTTCGGCGGGGAGTTCCTCCGCGACCAGCGTCAGCACCGTAAGCTCGGCGGTGGGTGTCGCGCTTGCGGCAACCGGTGGTGGTGATGGT                                                                                      >  SRR4302537.5760572/1‑94 (MQ=60)

         GTCGGTTTCGGCGGGGAGGTCCTCCGCGACCAGCGTCAGCACCGTAAGCTCGGCGGTGGGTGTCGCGCTTGCGGCAACCGGTGGTGGTGATGGT                                                                                      <  SRR4302537.3915742/94‑1 (MQ=60)

            GGTTTCGGCGGGGAGTTCCTCCGCGACCAGCGTCAGCACCGTAAGCTCGGCGGTGGGTGTCGCGCTTGCGGCAACCGGTGGTGGTGATGGTTCT                                                                                   <  SRR4302537.426722/94‑1 (MQ=60)

            GGTTTCGGCGGGGAGTTCCTCCGCGACCAGCGTCAGCACCGTAAGCTCGGCGGTGGGTGTCGCGCTTGCGGCAACCGGTGGTGGTGATGGTTCT                                                                                   >  SRR4302537.3268206/1‑94 (MQ=60)

             GTTTCGGCGGGGAGTTCCTCCGCGACCAGCGTCAGCACCGTAAGCTCGGCGGTGGGTGTCGCGCTTGCGGCAACCGGTGGTGGTGATGGTTCTA                                                                                  >  SRR4302537.5455584/1‑94 (MQ=60)

             GTTTCGGCGGGGAGTTCCTCCGCGACCAGCGTCAGCACCGTAAGCTCGGCGGTGGGTGTCGCGCTTGCGGCAACCGGTGGCGGTGATGGTTCTA                                                                                  >  SRR4302537.3874793/1‑94 (MQ=60)

             GTTTCGGCGGGGAGTTCCTCCGCGACCAGCGTCAGCACCGTAAGCTCGGCGGGGGGTGTCGCGCTTGCGGCAACCGGTGGTGGGGATGGTTCTA                                                                                  >  SRR4302537.5315036/1‑94 (MQ=60)

              TTTCGGCGGGGAGTTCCTCCGCGACCAGCGTCAGCACCGTAAGCTCGGCGGTGGGTGTCGCGCTTGCGGCAACCGGTGGTGGTGATGGTTCTAA                                                                                 >  SRR4302537.2244808/1‑94 (MQ=60)

                 CGGCGGGGAGTTCCTCCGCGACCAGCGTCAGCACCGTAAGCTCGGCGGTGGGTGTCGCGCTTGCGGCAACCGGTGGTGGTGATGGTTCTAATAC                                                                              <  SRR4302537.4478434/94‑1 (MQ=60)

                 CGGCGGGGAGTTCCTCCGCGACCAGCGTCAGCACCGTAAGCTCGGCGGTGGGTGTCGCGCTTGCGGCAACCGGTGGTGGTGATGGTTCTAATAC                                                                              <  SRR4302537.2179685/94‑1 (MQ=60)

                  GGCGGGGAGTTCCTCCGCGACCAGCGTCAGCACCGTAAGCTCGGCGGTGGGTGTCGCGCTTGCGGCAACCGGTGGTGGTGATGGTTCTAATACC                                                                             <  SRR4302537.2996117/94‑1 (MQ=60)

                  GGCGGGGAGTTCCTCCGCGACCAGCGTCAGCACCGTAAGCTCGGCGGTGGGTGTCGCGCTTGCGGCAACCGGTGGTGGTGATGGTTCTAATACC                                                                             <  SRR4302537.1931032/94‑1 (MQ=60)

                  GGCGGGGAGTTCCTCCGCGACCAGCGTCAGCACCGTAAGCTCGGCGGTGGGTGTCGCGCTTGCGGCAACCGGTGGTGGTGATGGTTCTAATACC                                                                             >  SRR4302537.3627289/1‑94 (MQ=60)

                   GCGGGGAGTTCCTCCGCGACCAGCGTCAGCACCGTAAGCTCGGCGGTGGGTGTCGCGCTTGCGGCAACCGGTGGTGGTGATGGTTCTAATACCG                                                                            >  SRR4302537.594519/1‑94 (MQ=60)

                    CGGGGAGTTCCTCCGCGACCAGCGTCAGCACCGTAAGCTCGGCGGTGGGTGTCGCGCTTGCGGCAACCGGTGGTGGTGATGGTTCTAATACCGG                                                                           >  SRR4302537.2571696/1‑94 (MQ=60)

                    CGGGGAGTTCCTCCGCGACCAGCGTCAGCACCGTAAGCTCGGCGGTGGGTGTCGCGCTTGCGGCAACCGGTGGTGGTGATGGTTCTAATACCGG                                                                           <  SRR4302537.532005/94‑1 (MQ=60)

                    CGGGGAGTTCCTCCGCGACCAGCGTCAGCACCGTAAGCTCGGCGGTGGGTGTCGCGCTTGCGGCAACCGGTGGTGGTGATGGTTCTAATACCGG                                                                           >  SRR4302537.2472252/1‑94 (MQ=60)

                    CGGGGAGTGCCTCCGCGACCAGCGTCAGCACCGTAAGCTCGGCGGTGGGTGTCGCGCTTGCGGCAACCGGTGGTGGTGATGGTTCTAATACCGG                                                                           <  SRR4302537.2355105/94‑1 (MQ=60)

                     gggtagtgcaTCCGCGACCAGCGTCAGCACCGTAAGCTCGGCGGTGGGTGTCGCGCTTGCGGCAACCGGTGGTGGTGATGGTTCTAATACCGGG                                                                          <  SRR4302537.2881684/84‑1 (MQ=60)

                     GGGGAGTTCCTCCGCGACCAGCGTCAGCACCGTAAGCTCGGCGGTGGGTGTCGCGCTTGCGGCAACCGGTGGTGGTGATGGTTCTAATACCGGG                                                                          >  SRR4302537.867313/1‑94 (MQ=60)

                     GGGGAGTTCCTCCGCGACCAGCGTCAGCACCGTAAGCTCGGCGGTGGGTGTCGCGCTTGCGGCAACCGGTGGTGGTGATGGTTCTAATACCGGG                                                                          <  SRR4302537.6141126/94‑1 (MQ=60)

                     GGGGAGTTCCTCCGCGACCAGCGTCAGCACCGTAAGCTCGGCGGTGGGTGTCGCGCTTGCGGCAACCGGTGGTGGTGATGGTTCTAATACCGGG                                                                          >  SRR4302537.2238247/1‑94 (MQ=60)

                      GGGAGTTCCTCCGCGACCAGCGTCAGCACCGTAAGCTCGGCGGTGGGTGTCGCGCTTGCGGCAACCGGTGGTGGTGATGGTTCTAATACCGGGA                                                                         >  SRR4302537.1905950/1‑94 (MQ=60)

                          GTTCCTCCGCGACCAGCGTCAGCACCGTAAGCTCGGCGGTGGGTGTCGCGCTTGCGGCAACCGGTGGTGGTGATGGTTCTAATACCGGGACCAC                                                                     >  SRR4302537.6055607/1‑94 (MQ=60)

                           TTCCTCCGCGACCAGCGTCAGCACCGTAAGCTCGGCGGTGGGTGTCGCGCTTGCGGCAACCGGTGGTGGTGATGGTTCTAATACCGGGACCACC                                                                    >  SRR4302537.635504/1‑94 (MQ=60)

                             CCTCCGCGACCAGCGTCAGCACCGTAAGCTCGGCGGTGGGTGTCGCGCTTGCGGCAACCGGTGGTGGTGATGGTTCTAATACCGGGACCACCAC                                                                  <  SRR4302537.455840/94‑1 (MQ=60)

                               TCCGCGACCAGCGTCAGCACCGTAAGCTCGGCGGAGGGTGTCGCGCTTGCGGCAACCGGTGGTGGTCGTGGTTCTAATACCGGGcacacaacaa                                                                >  SRR4302537.2884781/1‑84 (MQ=60)

                                CCGCGACCAGCGTCAGCACCGTAAGCTCGGCGGTGGGTGTCGCGCTTGCGGCAACCGGTGGTGGTGATGGTTCTAATACCGGGACCACCACAAC                                                               >  SRR4302537.5129925/1‑94 (MQ=60)

                                CCGCGACCAGCGTCAGCACCGTAAGCTCGGCGGTGGGTGTCGCGCTTGCGGCAACCGGTGGTGGTGATGGTTCTAATACCGGGACCACCACAAC                                                               >  SRR4302537.3154723/1‑94 (MQ=60)

                                  GCGACCAGCGTCAGCACCGTAAGCTCGGCGGTGGGTGTCGCGCTTGCGGCAACCGGTGGTGGTGATGGTTCTAATACCGGGACCACCACAACCA                                                             <  SRR4302537.325950/94‑1 (MQ=60)

                                    GACCAGCGTCAGCACCGTAAGCTCGGCGGTGGGTGTCGCGCTTGCGGCAACCGGTGGTGGTGATGGTTCTAATACCGGGACCACCACAACCACG                                                           <  SRR4302537.2319594/94‑1 (MQ=60)

                                    GACCAGCGTCAGCACCGTAAGCTCGGCGGTGGGTGTCGCGCTTGCGGCAACCGGTGGTGGTGATGGTTCTAATACCGGGACCACCACAACCACG                                                           <  SRR4302537.1303650/94‑1 (MQ=60)

                                    GACCAGCGTCAGCACCGTAAGCTCGGCGGTGGGTGTCGCGCTTGCGGCAACCGGTGGTGGTGATGGTTCTAATACCGGGACCACCACAACCACG                                                           <  SRR4302537.3183908/94‑1 (MQ=60)

                                     ACCAGCGTCAGCACCGTAAGCTCGGCGGTGGGTGTCGCGCTTGCGGCAACCGGTGGCGGTGATGGTTCTAATACCGGGACCACCACAACCACGA                                                          <  SRR4302537.3704567/94‑1 (MQ=60)

                                       CAGCGTCAGCACCGTAAGCTCGGCGGTGGGTGTCGCGCTTGCGGCAACCGGTGGTGGTGATGGTTCTAATACCGGGACCACCACAACCACGACC                                                        <  SRR4302537.2923921/94‑1 (MQ=60)

                                        AGCGTCAGCACCGTAAGCTCGGCGGTGGGTGTCGCGCTTGCGGCAACCGGTGGTGGTGATGGTTCTAATACCGGGACCACCACAACCACGACCA                                                       >  SRR4302537.3092337/1‑94 (MQ=60)

                                        AGCGTCAGCACCGTAAGCTCGGCGGTGGGTGTCGCGCTTGCGGCAACCGGTGGTGGTGATGGTTCTAATACCGGGACCACCACAACCACGACCA                                                       <  SRR4302537.1609937/94‑1 (MQ=60)

                                         GCGTCAGCACCGTAAGCTCGGCGGTGGGTGTCGCGCTTGCGGCAACCGGTGGTGGTGATGGTTCTAATACCGGGACCACCACAACCACGACCAC                                                      >  SRR4302537.2869592/1‑94 (MQ=60)

                                         GCGTCAGCACCGTAAGCTCGGCGGTGGGTGTCGCGCTTGCGGCAACCGGTGGTGGTGATGGTTCTAATACCGGGACCACCACAACCACGACCAC                                                      <  SRR4302537.58195/94‑1 (MQ=60)

                                          CGTCAGCACCGTAAGCTCGGCGGTGGGTGTCGCGCTTGCGGCAACCGGTGGTGGTGATGGTTCTAATACCGGGACCACCACAACCACGACCACC                                                     >  SRR4302537.517165/1‑94 (MQ=60)

                                           GTCAGCACCGTAAGCTCGGCGGTGGGTGTCGCGCTTGCGGCAACCGGTGGTGGTGATGGTTCTAATACCGGGACCACCACAACCACGACCACCA                                                    >  SRR4302537.5781142/1‑94 (MQ=60)

                                            TCAGCACCGTAAGCTCGGCGGTGGGTGTCGCGCTTGCGGCAACCGGTGGTGGTGATGGTTCTAATACCGGGACCACCACAACCACGACCACCAG                                                   <  SRR4302537.2714737/94‑1 (MQ=60)

                                            TCAGCACCGTAAGCTCGGCGGTGGGTGTCGCGCTTGCGGCAACCGGTGGTGGTGATGGTTCTAATACCGGGACCACCACAACCACGACCACCAG                                                   <  SRR4302537.3535533/94‑1 (MQ=60)

                                             CAGCACCGTAAGCTCGGCGGTGGGTGTCGCGCTTGCGGCAACCGGTGGTGGTGATGGTTCTAATACCGGGACCACCACAACCACGACCACCAGT                                                  >  SRR4302537.2694866/1‑94 (MQ=60)

                                             CAGCACCGTAAGCTCGGCGGTGGGTGTCGCGCTTGCGGCAACCGGTGGTGGTGATGGTTCTAATACCGGGACCACCACAACCACGACCACCAGT                                                  <  SRR4302537.713240/94‑1 (MQ=60)

                                                CACCGTAAGCTCGGCGGTGGGTGTCGCGCTTGCGGCAACCGGTGGTGGTGATGGTTCTAATACCGGGACCACCACAACCACGACCACCAGTACC                                               <  SRR4302537.1435141/94‑1 (MQ=60)

                                                 ACCGTAAGCTCGGCGGTGGGTGTCGCGCTTGCGGCAACCGGTGGTGGTGATGGTTCTAATACCGGGACCACCACAACCACGACCACCAGTACCC                                              >  SRR4302537.1720209/1‑94 (MQ=60)

                                                 ACCGTAAGCTCGGCGGTGGGTGTCGCGCTTGCGGCAACCGGTGGTGGTGATGGTTCTAATACCGGGACCACCACAACCACGACCACCAGTACCC                                              <  SRR4302537.797233/94‑1 (MQ=60)

                                                 ACCGTAAGCTCGGCGGTGGGTGTCGCGCTTGCGGCAACCGGTGGTGGTGATGGTTCTAATACCGGGACCACCACAACCACGACCACCAGTACCC                                              >  SRR4302537.3693069/1‑94 (MQ=60)

                                                   CGTAAGCTCGGCGGTGGGTGTCGCGCTTGCGGCAACCGGTGGTGGTGATGGTTCTAATCCCGGGACCACCACAACCACGACCACCAGTACCCAG                                            <  SRR4302537.691776/94‑1 (MQ=60)

                                                   CGTAAGCTCGGCGGTGGGTGTCGCGCTTGCGGCAACCGGTGGTGGTGATGGTTCTAATACCGGGACCACCACAACCACGACCACCAGTACCCAG                                            <  SRR4302537.4464330/94‑1 (MQ=60)

                                                   CGTAAGCTCGGCGGTGGGTGTCGCGCTTGCGGCAACCGGTGGTGGTGATGGTTCTAATACCGGGACCACCACAACCACGACCACCAGTACCCAG                                            <  SRR4302537.1757458/94‑1 (MQ=60)

                                                   CGTAAGCTCGGCGGTGGGTGTCGCGCTTGCGGCAACCGGTGGTGGTGATGGTTCTAATACCGGGACCACCACAACCACGACCACCAGTACCCAG                                            <  SRR4302537.5879502/94‑1 (MQ=60)

                                                   CGTAAGCTCGGCGGTGGGTGTCGCGCTTGCGGCAACCGGTGGTGGTGATGGTTCTAATACCGGGACCACCACAACCACGACCACCAGTACCCAG                                            <  SRR4302537.6124134/94‑1 (MQ=60)

                                                   CGTAAGCTCGGCGGTGGGTGTCGCGCTTGCGGCAACCGGTGGTGGTGATGGTTCTAATACCGGGACCACCACAACCACGACCACCAGTACCCAG                                            <  SRR4302537.6263845/94‑1 (MQ=60)

                                                    GTAAGCTCGGCGCTGGGTGTCGCGCTTGCGGCAACCGGTGGTGGTGATGGTTCTAATACCGGGACCACCACAACCACGACCACCAGTACCCAGT                                           <  SRR4302537.875753/94‑1 (MQ=60)

                                                     TAAGCTCGGCGGTGGGTGTCGCGCTTGCGGCAACCGGTGGTGGTGATGGTTCTAATACCGGGACCACCACAACCACGACCACCAGTACCCAGTA                                          <  SRR4302537.3078456/94‑1 (MQ=60)

                                                      AAGCTCGGCGGTGGGTGTCGCGCTTGCGGCAACCGGTGGTGGTGATGGTTCTAATACCGGGACCACCACAACCACGACCACCAGTACCCAGTAA                                         <  SRR4302537.2544538/94‑1 (MQ=60)

                                                       AGCTCGGCGGTGGGTGTCGCGCTTGCGGCAACCGGTGGTGGTGATGGTTCTAATACCGGGACCACCACAACCACGACCACCAGTACCCAGTAAT                                        <  SRR4302537.5347283/94‑1 (MQ=60)

                                                       AGCTCGGCGGTGGGTGTCGCGCTTGCGGCAACCGGTGGTGGTGATGGTTCTAATACCGGGACCACCACAACCACGACCACCAGTACCCAGTAAT                                        >  SRR4302537.1873745/1‑94 (MQ=60)

                                                        GCTCGGCGGTGGGTGTCGCGCTTGCGGCAACCGGTGGTGGTGATGGTTCTAATACCGGGACCACCACAACCACGACCACCAGTACCCAGTAATA                                       <  SRR4302537.3282112/94‑1 (MQ=60)

                                                         CTCGGCGGTGGGTGTCGCGCTTGCGGCAACCGGTGGTGGTGATGGTTCTAATACCGGGACCACCACAACCACGACCACCAGTACCCAGTAATAA                                      <  SRR4302537.659070/94‑1 (MQ=60)

                                                            GGCGGTGGGTGTCGCGCTTGCGGCAACCGGTGGTGGTGATGGTTCTAATACCGGGACCACCACAACCACGACCACCAGTACCCAGTAATAAAGT                                   >  SRR4302537.3401801/1‑94 (MQ=60)

                                                            GGCGGTGGGTGTCGCGCTTGCGGCAACCGGTGGTGGTGATGGTTCTAATACCGGGACCACCACAACCACGACCACCAGTACCCAGTAATAAAGT                                   <  SRR4302537.4569360/94‑1 (MQ=60)

                                                            GGCGGTGGGTGTCGCGCTTGCGGCAACCGGTGGTGGTGATGGTTCTAATACCGGGACCACCACAACCACGACCACCAGTACCCAGTAATAAAGT                                   <  SRR4302537.5147619/94‑1 (MQ=60)

                                                            GGCGGTGGGTGTCGCGCTTGCGGCAACCGGTGGTGGTGATGGTTCTAATACAGGGACCACCACAACACCGACTACCAGTACCCAGTAATAAAGT                                   >  SRR4302537.5598150/1‑94 (MQ=60)

                                                            GGCGGTGGGTGTCGCGCTTGCGGCAACCGGTGGCGGTGATGGTTCTAATACCGGGACCACCACAACCACGACCACCAGTACCCAGTAATAAAGT                                   <  SRR4302537.6125626/94‑1 (MQ=60)

                                                             GCGGTGGGTGTCGCGCTTGCGGCAACCGGTGGTGGTGATGGTTCTAATACCGGGACCACCACAACCACGACCACCAGTACCCAGTAATAAAGTA                                  <  SRR4302537.2732506/94‑1 (MQ=60)

                                                              CGGTGGGTGTCGCGCTTGCGGCAACCGGTGGTGGTGATGGTTCTAATACCGGGACCACCACAACCACGACCACCAGTACCCAGTAATAAAGTAT                                 <  SRR4302537.369923/94‑1 (MQ=60)

                                                              CGGTGGGTGTCGCGCTTGCGGCAACCGGTGGTGGTGATGGTTCTAATACCGGGACCACCACAACCACGACCACCAGTACCCAGTAATAAAGTAT                                 <  SRR4302537.5508381/94‑1 (MQ=60)

                                                              CGGTGGGTGTCGCGCTTGCGGCAACCGGTGGTGGTGATGGTTCTAATACCGGGACCACCACAACCACGACCACCAGTACCCAGTAATAAAGTAT                                 >  SRR4302537.3229999/1‑94 (MQ=60)

                                                               GGTGGGTGTCGCGCTTGCGGCAACCGGTGGTGGTGATGGTTCTAATACCGGGACCACCACAACCACGACCACCAGTACCCAGTAATAAAGTATG                                >  SRR4302537.5054129/1‑94 (MQ=60)

                                                               GGTGGGTGTCGCGCTTGCGGCAACCGGTGGTGGTGATGGTTCTAATACCGGGACCACCACAACCACGACCACCAGTACCCAGTAATAAAGTATG                                >  SRR4302537.5628725/1‑94 (MQ=60)

                                                                GTGGGTGTCGCGCTTGCGGCAACCGGTGGTGGTGATGGTTCTAATACCGGGACCACCACAACCACGACCACCAGTACCCAGTAATAAAGTATGT                               <  SRR4302537.977340/94‑1 (MQ=60)

                                                                GTGGGTGTCGCGCTTGCGGCAACCGGTGGTGGTGATGGTTCTAATACCGGGACCACCACAACCACGACCACCAGTACCCAGTAATAAAGTATGT                               <  SRR4302537.6337033/94‑1 (MQ=60)

                                                                  GGGTGTCGCGCTTGCGGCAACCGGTGGTGGTGATGGTTCTAATACCGGGACCACCACAACCACGACCACCAGTACCCAGTAATAAAGTATGTAT                             >  SRR4302537.293860/1‑94 (MQ=60)

                                                                  GGGTGTCGCGCTTGCGGCAACCGGTGGTGGTGATGGTTCTAATACCGGGACCACCACAACCACGACCACCAGTACCCAGTAATAAAGTATGTAT                             >  SRR4302537.1206774/1‑94 (MQ=60)

                                                                   GGTGTCGCGCTTGCGGCAACCGGTGGTGGTGATGGTTCTAATACCGGGACCACCACAACCACGACCACCAGTACCCAGTAATAAAGTATGTATC                            >  SRR4302537.4156790/1‑94 (MQ=60)

                                                                   GGTGTCGCGCTTGCGGCAACCGGTGGTGGTGATGGTTCTAATACCGGGACCACCACAACCACGACCACCAGTACCCAGTAATAAAGTATGTATC                            <  SRR4302537.4227688/94‑1 (MQ=60)

                                                                   GGTGTCGCGCTTGCGGCAACCGGTGGTGGTGATGGTTCTAATACCGGGACCACCACAACCACGACCACCAGTACCCAGTAATAAAGTATGTATC                            <  SRR4302537.3142440/94‑1 (MQ=60)

                                                                   GGTGTCGCGCTTGCGGCAACCGGTGGTGGTGATGGTTCTAATACCGGGACCACCACAACCACGACCACCAGTACCCAGTAATAAAGTATGTATC                            <  SRR4302537.2218265/94‑1 (MQ=60)

                                                                    GTGTCGCGCTTGCGGCAACCGGTGGTGGTGATGGTTCTAATACCGGGACCACCACAACCACGACCACCAGTACCCAGTAATAAAGTATGTATCC                           <  SRR4302537.1512473/94‑1 (MQ=60)

                                                                     TGTCGCGCTTGCGGCAACCGGTGGTGGTGATGGTTCTAATACCGGGACCCCCACAACCACGACCACCAGTACCCAGTAATAAAGTATGTATCCC                          <  SRR4302537.269303/94‑1 (MQ=60)

                                                                          CGCTTGCGGCAACCGGTGGTGGTGATGGTTCTAATACCGGGACCACCACAACCACGACCACCAGTACCCAGTAATAAAGTATGTATCCCCAAAA                     <  SRR4302537.3833818/94‑1 (MQ=60)

                                                                          CGCTTGCGGCAACCGGTGGTGGTGATGGTTCTAATACCGGGACCACCACAACCACGACCACCAGTACCCAGTAATAAAGTATGTATCCCCAAAA                     >  SRR4302537.1746355/1‑94 (MQ=60)

                                                                          CGCTTGCGGCAACCGGTGGTGGTGATGGTTCTAATACCGGGACCACCACAACCACGACCACCAGTACCCAGTAATAAAGTATGTATCCCCAAAA                     >  SRR4302537.2788035/1‑94 (MQ=60)

                                                                          CGCTTGCGGCAACCGGTGGTGGTGATGGTTCTAATACCGGGACCACCACAACCACGACCACCAGTACCCAGTAATAAAGTATGTATCCCCAAAA                     <  SRR4302537.6435055/94‑1 (MQ=60)

                                                                           GCTTGCGGCAACCGGTGGTGGTGATGGTTCTAATACCGGGACCACCACAACCACGACCACCAGTACCCAGTAATAAAGTATGTATCCCCAAAAT                    >  SRR4302537.4124460/1‑94 (MQ=60)

                                                                            CTTGCGGCAACCGGTGGTGGTGATGGTTCTAATACCGGGACCACCACAACCACGACCACCAGTACCCAGTAATAAAGTATGTATCCCCAAAATA                   >  SRR4302537.927232/1‑94 (MQ=60)

                                                                            CTTGCGGCAACCGGTGGTGGTGATGGTTCTAATACCGGGACCACCACAACCACGACCACCAGTACCCAGTAATAAAGTATGTATCCCCAAAATA                   <  SRR4302537.4875303/94‑1 (MQ=60)

                                                                              TGCGGCAACCGGTGGTGGTGATGGTTCTAATACCGGGACCACCACAACCACGACCACCAGTACCCATTAATAAAGTATGTATCCCCAAAATAAT                 <  SRR4302537.4185505/94‑1 (MQ=60)

                                                                               GCGGCAACCGGTGGTGGTGATGGTTCTAATACCGGGACCACCACAACCACGACCACCAGTACCCAGTAATAAAGTATGTATCCCCAAAATAATT                <  SRR4302537.4964880/94‑1 (MQ=60)

                                                                                CGGCAACCGGTGGTGGTGATGGTTCTAATACCGGGACCACCACAACCACGACCACCAGTACCCAGTAATAAAGTATGTATCCCCAAAATAATTC               <  SRR4302537.2281950/94‑1 (MQ=60)

                                                                                CGGCAACCGGTGGTGGTGATGGTTCTAATACCGGGACCACCACAACCACGACCACCAGTACCCAGTAATAAAGTATGTATCCCCAAAATAATTC               <  SRR4302537.4568702/94‑1 (MQ=60)

                                                                                CGGCAACCGGTGGTGGTGATGGTTCTAATACCGGGACCACCACAACCACGACCACCAGTACCCAGTAATAAAGTATGTATCCCCAAAATAATTC               <  SRR4302537.6398632/94‑1 (MQ=60)

                                                                                 GGCAACCGGTGGTGGTGATGGTTCTAATACCGGGACCACCACAACCACGAGCACCAGTACCCAGTAATAAAGTATGTATCCCCAAAATAATTCG              >  SRR4302537.3725267/1‑94 (MQ=60)

                                                                                 GGCAACCGGTGGTGGTGATGGTTCTAATACCGGGACCACCACAACCACGACCACCAGTACCCAGTAATAAAGTATGTATCCCCAAAATAATTCG              <  SRR4302537.606323/94‑1 (MQ=60)

                                                                                 GGCAACCGGTGGTGGTGATGGTTCTAATACCGGGACCACCACAACCACGACCACCAGTACCCAGTAATAAAGTATGTATCCCCAAAATAATTCG              <  SRR4302537.146969/94‑1 (MQ=60)

                                                                                   CAACCGGTGGTGGTGATGGTTCTAATACCGGGACCACCACAACCACGACCACCAGTACCCAGTAATAAAGTATGTATCCCCAAAATAATTCGAG            >  SRR4302537.1052306/1‑94 (MQ=60)

                                                                                   CAACCGGTGGTGGTGATGGTTCTAATACCGGGACCACCACAACCACGACCACCAGTACCCAGTAATAAAGTATGTATCCCCAAAATAATTCGAG            >  SRR4302537.1723791/1‑94 (MQ=60)

                                                                                    AACCGGTGGTGGTGATGGTTCTAATACCGGGACCACCACAACCACGACCACCAGTACCCAGTAATAAAGTATGTATCCCCAAAATAATTCGAGT           >  SRR4302537.6470218/1‑94 (MQ=60)

                                                                                    AACCGGTGGCGGTGATGGTTCTAATACCGGGACCACCACAACCACGACCACCAGTACCCAGTAATAAAGTATGTATCCCCAAAATAATTCGAGT           <  SRR4302537.4455914/94‑1 (MQ=60)

                                                                                     ACCGGTGGTGGTGATGGTTCTAATACCGGGACCACCACAACCACGACCACCACTACCCAGTAATAAAGTATGTATCCCCAAAATAATTCGAGTC          <  SRR4302537.4339697/94‑1 (MQ=60)

                                                                                      CCGGTGGTGGTGATGGTTCTAATACCGGGACCACCACAACCACGACCACCAGTACCCAGTAATAAAGTATGTATCCCCAAAATAATTCGAGTCA         <  SRR4302537.1788044/94‑1 (MQ=60)

                                                                                      CCGGTGGTGGTGATGGTTCTAATACCGGGACCACCACAACCACGACCACCAGTACCCAGTAATAAAGTATGTATCCCCAAAATAATTCGAGTCA         >  SRR4302537.2310749/1‑94 (MQ=60)

                                                                                        GGTGGTGGTGATGGTTCTAATACCGGGACCACCACAACCACGACCACCAGTACCCAGTAATAAAGTATGTATCCCCAAAATAATTCGAGTCATT       <  SRR4302537.1265170/94‑1 (MQ=60)

                                                                                        GGTGGTGGTGATGGTTCTAATACCGGGACCACCACAACCACGACCACCAGTACCCAGTAATAAAGTATGTATCCCCAAAATAATTCGAGTCATT       <  SRR4302537.4255132/94‑1 (MQ=60)

                                                                                         GTGGTGGTGATGGTTCTAATACCGGGACCACCACAACCACGACCACCAGTACCCAGTAATAAAGTATGTATCCCCAAAATAATTCGAGTCATTG      <  SRR4302537.355548/94‑1 (MQ=60)

                                                                                          TGGTGGTGATGGTTCTAATACCGGGACCACCACAACCACGACCACCAGTACCCAGTAATAAAGTATGTATCCCCAAAATAATTCGAGTCATTGC     <  SRR4302537.2197785/94‑1 (MQ=60)

                                                                                           GGTGGTGATGGTTCTAATACCGGGACCACCACAACCACGACCACCAGTACCCAGTAATAAAGTATGTATCCCCAAAATAATTCGAGTCATTGCA    >  SRR4302537.6411659/1‑94 (MQ=60)

                                                                                           GGTGGTGATGGTTCTAATACCGGGACCACCACAACCACGACCACCAGTACCCAGTAATAAAGTATGTATCCCCAAAATAATTCGAGTCATTGCA    >  SRR4302537.6440700/1‑94 (MQ=60)

                                                                                           GGTGGTGATGGTTCTAATACCGGGACCACCACAACCACGACCACCAGTACCCAGTAATAAAGTATGTATCCCCAAAATAATTCGAGTCATTGCA    <  SRR4302537.3189802/94‑1 (MQ=60)

                                                                                             TGGTGATGGTTCTAATACCGGGACCACCACAACCACGACCACCAGTACCCAGTAATAAAGTATGTATCCCCAAAATAATTCGAGTCATTGCATC  <  SRR4302537.1784603/94‑1 (MQ=60)

                                                                                             TGGTGATGGTTCTAATACCGGGACCACCACAACCACGACCACCAGTACCCAGTAATAAAGTATGTATCCCCAAAATAATTCGAGTCATTGCATC  <  SRR4302537.308780/94‑1 (MQ=60)

GGCAGCAACGTCGGTTTCGGCGGGGAGTTCCTCCGCGACCAGCGTCAGCACCGTAAGCTCGGCGGTGGGTGTCGCGCTTGCGGCAACCGGTGGCGGTGATGGTTCTAATACCGGGACCACCACAACCACGACCACCAGTACCCAGTAATAAAGTATGTATCCCCAAAATAATTCGAGTCATTGCATC  >  NC_000913/4233580‑4233766

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`