Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 882 254.4 12957588 98.0% 12698436 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,316,203 T→A Q47L (CAG→CTG)  phnK ← ATP‑binding component of phosphonate transport

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,316,2030TA100.0% 852.1 / NA 238Q47L (CAG→CTG) phnKATP‑binding component of phosphonate transport
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (118/120);  total (118/120)

Only 100 of 252 total aligned reads displayed.

GGTCGGCCTCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCTGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACATCGCTAAAGCCTTTGCCC  >  NC_000913/4316114‑4316292
                                                                                         |                                                                                         
ggTCGGCCTCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCg                                                                                        <  1:4157974/93‑1 (MQ=255)
ggTCGGCCTCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCg                                                                                        >  1:1183909/1‑93 (MQ=255)
ggTCGGCCTCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCg                                                                                        <  2:3500313/93‑1 (MQ=255)
ggTCGGCCTCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCg                                                                                        >  2:4753242/1‑93 (MQ=255)
ggTCGGCCTCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCg                                                                                        <  2:247390/93‑1 (MQ=255)
 gTCGGCCTCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGt                                                                                       >  2:5722797/1‑93 (MQ=255)
 gTCGGCCTCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGt                                                                                       <  2:2981891/93‑1 (MQ=255)
  tCGGCCTCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTg                                                                                      <  1:1016263/93‑1 (MQ=255)
  tCGGCCTCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTg                                                                                      >  2:2378108/1‑93 (MQ=255)
    ggCCTCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGt                                                                                    >  1:2685778/1‑93 (MQ=255)
      ccTCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCt                                                                                  >  1:3329265/1‑93 (MQ=255)
        tCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTc                                                                                >  1:650880/1‑93 (MQ=255)
        tCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTc                                                                                >  1:6036167/1‑93 (MQ=255)
        tCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTc                                                                                <  1:4499111/93‑1 (MQ=255)
         cGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTcc                                                                               >  1:2497238/1‑93 (MQ=255)
         cGCTCATTGCATACAGCGAACGGGTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTcc                                                                               >  2:3190934/1‑93 (MQ=255)
          gCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTccc                                                                              <  2:1460236/93‑1 (MQ=255)
          gCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGCGGTCTTccc                                                                              >  1:2672205/1‑93 (MQ=255)
           cTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCg                                                                             >  1:1577250/1‑93 (MQ=255)
            tCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCgg                                                                            >  2:1185507/1‑93 (MQ=255)
             cATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGa                                                                           <  2:5131805/93‑1 (MQ=255)
              aTTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAg                                                                          >  2:398483/1‑93 (MQ=255)
               ttGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGc                                                                         <  2:1891489/93‑1 (MQ=255)
                tGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGcc                                                                        <  2:4449928/93‑1 (MQ=255)
                 gCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCg                                                                       <  1:5594475/93‑1 (MQ=255)
                 gCATACAGCGAACGGTTCTCGTAGTGAATTCCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCg                                                                       <  1:4832967/93‑1 (MQ=255)
                  cATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCgg                                                                      >  1:3278925/1‑93 (MQ=255)
                   aTACAGCGAACGGTTCTCGTAGTGAATTTTCCCCTGCTGCGGCGTCAGGCGCGCGGAGGTCGACTTCAGCAGCGTGGTCTTCACGGCGCCggt                                                                     >  1:2261530/1‑92 (MQ=255)
                   aTACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGa                                                                     >  2:5660914/1‑93 (MQ=255)
                   aTACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGa                                                                     >  1:1645684/1‑93 (MQ=255)
                   aTACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGa                                                                     >  2:3623511/1‑93 (MQ=255)
                     aCAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGAtt                                                                   <  1:794193/93‑1 (MQ=255)
                       aGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTcc                                                                 >  1:2806566/1‑93 (MQ=255)
                        gCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTccc                                                                <  2:6353888/93‑1 (MQ=255)
                         cGAACGGTTGTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTcccc                                                               <  1:5978604/93‑1 (MQ=255)
                         cGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTcccc                                                               <  2:2613695/93‑1 (MQ=255)
                          gAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCg                                                              >  2:6170187/1‑93 (MQ=255)
                            aCGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGAc                                                            >  1:6415929/1‑93 (MQ=255)
                               gTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAAt                                                         >  2:3766526/1‑93 (MQ=255)
                                ttCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATg                                                        >  1:4870258/1‑93 (MQ=255)
                                  ctcGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGcc                                                      >  1:5412606/1‑93 (MQ=255)
                                  ctcGTAGTGAATTTCCCCCCGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGcc                                                      >  2:6326618/1‑93 (MQ=255)
                                   tcGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGccc                                                     <  1:471384/93‑1 (MQ=255)
                                    cGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCa                                                    <  1:2334496/93‑1 (MQ=255)
                                     gTAGTGAATTTCCCCCTGATGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAg                                                   <  1:3442535/93‑1 (MQ=255)
                                       aGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCa                                                 <  1:1998283/93‑1 (MQ=255)
                                        gTGAATTTACCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCAc                                                <  2:5547457/93‑1 (MQ=255)
                                         tGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACt                                               >  2:5048473/1‑93 (MQ=255)
                                         tGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCCGAGCCGGATTCCACGACACTGCCCAGCACt                                               >  2:406846/1‑93 (MQ=255)
                                          gAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACtt                                              <  1:3189232/93‑1 (MQ=255)
                                            aTTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTcc                                            <  2:4186769/93‑1 (MQ=255)
                                             tttCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTccc                                           >  1:5134319/1‑93 (MQ=255)
                                             atgcccccTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTccc                                           <  1:3874897/90‑1 (MQ=255)
                                               tCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTccccc                                         >  2:2226601/1‑93 (MQ=255)
                                               tCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTccacc                                         <  1:1324456/93‑1 (MQ=255)
                                                cccccTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCg                                        >  2:2724038/1‑93 (MQ=255)
                                                  cccTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGc                                      >  1:3840312/1‑93 (MQ=255)
                                                   ccTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGcc                                     <  1:3963209/93‑1 (MQ=255)
                                                   ccTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGcc                                     >  2:2877999/1‑93 (MQ=255)
                                                   ccTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGGCTTCCCGGAGCCGGCTTCCCCGACAATGCCCAGCACTTCCCCCTGcc                                     >  2:4424210/1‑93 (MQ=255)
                                                     tgctgcGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCAt                                   >  2:3529676/1‑93 (MQ=255)
                                                      gctgcGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATa                                  <  2:4724082/93‑1 (MQ=255)
                                                      gctgcGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATa                                  <  1:2925240/93‑1 (MQ=255)
                                                      gctgcGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATa                                  <  2:3114238/93‑1 (MQ=255)
                                                       ctgcGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATaa                                 >  2:1710734/1‑93 (MQ=255)
                                                       ctgcGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATaa                                 <  1:3214683/93‑1 (MQ=255)
                                                         gcggcgTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAAt                               <  2:1089836/93‑1 (MQ=255)
                                                              gtaaGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCaaaa                          >  1:2580384/4‑93 (MQ=255)
                                                              gTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCaaaa                          >  1:1358529/1‑93 (MQ=255)
                                                                cAGGCGCTCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAaga                        <  2:5475102/93‑1 (MQ=255)
                                                                cAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAaga                        <  2:4664399/93‑1 (MQ=255)
                                                                cAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAaga                        <  1:5174021/93‑1 (MQ=255)
                                                                cAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGAATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAaga                        <  2:802076/93‑1 (MQ=255)
                                                                 aGGCGCGCTGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAagag                       >  2:6297484/1‑93 (MQ=255)
                                                                 aGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAagag                       <  2:458690/93‑1 (MQ=255)
                                                                  ggCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAgaga                      >  2:901502/1‑93 (MQ=255)
                                                                  ggCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAgaga                      >  2:5798773/1‑93 (MQ=255)
                                                                  ggCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAgaga                      <  2:5391295/93‑1 (MQ=255)
                                                                  ggCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAgaga                      >  1:1274845/1‑93 (MQ=255)
                                                                  ggCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAgaga                      <  1:2040617/93‑1 (MQ=255)
                                                                    cgcgcgGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACa                    <  1:2769720/93‑1 (MQ=255)
                                                                     gcgcgGAGATCGACTTCAGCAGCGTGGTCTCCCCGGAGCCGGATCCCCCGACAATGCCCAGCTCTTCCCCCGGCCATAAATCAAAAGAGACAt                   <  2:5568534/93‑1 (MQ=255)
                                                                      cgcgGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGCGACATc                  >  2:2167943/1‑93 (MQ=255)
                                                                        cgGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACATCGc                <  1:6397826/93‑1 (MQ=255)
                                                                        cgGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACATCGc                <  1:3647106/93‑1 (MQ=255)
                                                                          gagaTCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACATCGCTa              >  1:3093629/1‑93 (MQ=255)
                                                                           agaTCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACATCGCTaa             >  2:731368/1‑93 (MQ=255)
                                                                            gaTCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACATCGCTaaa            >  1:4767687/1‑93 (MQ=255)
                                                                             aTCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACATCGCTAAAg           <  1:1132317/93‑1 (MQ=255)
                                                                              tCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACATCGCTAAAGc          >  1:2987169/1‑93 (MQ=255)
                                                                              tCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACATCGCTAAAGc          <  2:6051192/93‑1 (MQ=255)
                                                                                gACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACATCGCTCAAGCCt        >  1:1059878/1‑93 (MQ=255)
                                                                                gACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACATCGCTAAAGCCt        <  1:130273/93‑1 (MQ=255)
                                                                                gACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACATCGCTAAAGCCt        <  1:888255/93‑1 (MQ=255)
                                                                                 aCTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACATCGCTAAAGCCtt       <  2:5875723/93‑1 (MQ=255)
                                                                                 aCTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACATCGCTAAAGCCtt       <  1:3416971/93‑1 (MQ=255)
                                                                                  cTTCAGCAGGGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACATCGCTAAAGCCttt      <  2:6120434/93‑1 (MQ=255)
                                                                                  cTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACATCGCTAAAGCCttt      <  1:4597042/93‑1 (MQ=255)
                                                                                    tCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACATCGCTAAAGCCTTTGc    <  1:4312126/93‑1 (MQ=255)
                                                                                      agcagcGTGGTCTTCCCGGAGCCGGATACCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACATCGCTAAAGCCTTTGccc  <  2:2048797/93‑1 (MQ=255)
                                                                                         |                                                                                         
GGTCGGCCTCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCTGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACATCGCTAAAGCCTTTGCCC  >  NC_000913/4316114‑4316292

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CGGCGGTCGGCCTCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCTGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACATCGCTAAAGCCTTTGCCCGGC  >  NC_000913/4316110‑4316295
                                                                                             |                                                                                            
CGGCGGTCGGCCTCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCA                                                                                              <  SRR4302537.268851/94‑1 (MQ=60)
 GGCGGTCGGCCTCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAG                                                                                             >  SRR4302537.1735194/1‑94 (MQ=60)
   CGGTCGGCCTCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCG                                                                                           >  SRR4302537.1192140/1‑94 (MQ=60)
   CGGTCGGCCTCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCG                                                                                           >  SRR4302537.2719613/1‑94 (MQ=60)
    GGTCGGCCTCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGT                                                                                          <  SRR4302537.4187011/94‑1 (MQ=60)
    GGTCGGCCTCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGT                                                                                          >  SRR4302537.262153/1‑94 (MQ=60)
     GTCGGCCTCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTG                                                                                         <  SRR4302537.4286368/94‑1 (MQ=60)
     GTCGGCCTCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTG                                                                                         <  SRR4302537.1092522/94‑1 (MQ=60)
      TCGGCCTCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGG                                                                                        <  SRR4302537.1023262/94‑1 (MQ=60)
      TCGGCCTCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGG                                                                                        <  SRR4302537.4695866/94‑1 (MQ=60)
       CGGCCTCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGT                                                                                       >  SRR4302537.2704523/1‑94 (MQ=60)
        GGCCTCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTC                                                                                      >  SRR4302537.2791767/1‑94 (MQ=60)
        GGCCTCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTC                                                                                      <  SRR4302537.253290/94‑1 (MQ=60)
         GCCTCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCT                                                                                     >  SRR4302537.3352822/1‑94 (MQ=60)
         GCCTCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCT                                                                                     <  SRR4302537.982211/94‑1 (MQ=60)
          CCTCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTT                                                                                    >  SRR4302537.1587832/1‑94 (MQ=60)
           CTCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTC                                                                                   >  SRR4302537.3689391/1‑94 (MQ=60)
           CTCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTC                                                                                   >  SRR4302537.655314/1‑94 (MQ=60)
           CTCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTC                                                                                   >  SRR4302537.6077640/1‑94 (MQ=60)
           CTCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGGNGTCTTC                                                                                   >  SRR4302537.269133/1‑94 (MQ=60)
            TCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCC                                                                                  <  SRR4302537.4530228/94‑1 (MQ=60)
            TCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCC                                                                                  >  SRR4302537.2514814/1‑94 (MQ=60)
             CGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCC                                                                                 <  SRR4302537.2035480/94‑1 (MQ=60)
             CGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGCGGTCTTCCC                                                                                 >  SRR4302537.2690870/1‑94 (MQ=60)
              GCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCG                                                                                <  SRR4302537.294301/94‑1 (MQ=60)
              GCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCG                                                                                >  SRR4302537.1588249/1‑94 (MQ=60)
              GCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCG                                                                                >  SRR4302537.1497592/1‑94 (MQ=60)
               CTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGG                                                                               <  SRR4302537.4464272/94‑1 (MQ=60)
               CTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGG                                                                               >  SRR4302537.3050823/1‑94 (MQ=60)
                   TTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCC                                                                           <  SRR4302537.2404962/94‑1 (MQ=60)
                    TGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCG                                                                          >  SRR4302537.4125905/1‑94 (MQ=60)
                     GCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGG                                                                         >  SRR4302537.3302072/1‑94 (MQ=60)
                     GCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGG                                                                         <  SRR4302537.5632725/94‑1 (MQ=60)
                     GCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGG                                                                         >  SRR4302537.6121164/1‑94 (MQ=60)
                     GCATACAGCGAACGGTTCTCGTAGTGAATTCCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGG                                                                         <  SRR4302537.4866340/94‑1 (MQ=60)
                      CATACAGCGAACGGTTCTCGTAGTGAATTTTCCCCTGCTGCGGCGTCAGGCGCGCGGAGGTCGACTTCAGCAGCGTGGTCTTCACGGCGCCGGT                                                                        >  SRR4302537.2277489/1‑94 (MQ=60)
                      CATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGA                                                                        >  SRR4302537.1657213/1‑94 (MQ=60)
                       ATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGAT                                                                       >  SRR4302537.2273817/1‑94 (MQ=60)
                        TANANCNAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATT                                                                      <  SRR4302537.5665145/94‑1 (MQ=60)
                        TACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATT                                                                      <  SRR4302537.1315552/94‑1 (MQ=60)
                        TACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATT                                                                      <  SRR4302537.2221888/94‑1 (MQ=60)
                         ACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTC                                                                     <  SRR4302537.799644/94‑1 (MQ=60)
                         ACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTC                                                                     >  SRR4302537.3562507/1‑94 (MQ=60)
                         ACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTC                                                                     <  SRR4302537.2753836/94‑1 (MQ=60)
                          CAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCC                                                                    >  SRR4302537.2826185/1‑94 (MQ=60)
                           AGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCC                                                                   >  SRR4302537.4618140/1‑94 (MQ=60)
                             CGAACGGTTGTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCG                                                                 <  SRR4302537.6019643/94‑1 (MQ=60)
                             CGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCG                                                                 <  SRR4302537.1613152/94‑1 (MQ=60)
                              GAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGA                                                                >  SRR4302537.2329830/1‑94 (MQ=60)
                              GAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGA                                                                >  SRR4302537.6447823/1‑94 (MQ=60)
                              GAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGA                                                                >  SRR4302537.5016402/1‑94 (MQ=60)
                               AACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGAC                                                               <  SRR4302537.555793/94‑1 (MQ=60)
                               AACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGAC                                                               >  SRR4302537.6026461/1‑94 (MQ=60)
                               AACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGAC                                                               >  SRR4302537.6459565/1‑94 (MQ=60)
                                 CGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAA                                                             >  SRR4302537.3392657/1‑94 (MQ=60)
                                 CGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAA                                                             >  SRR4302537.2458534/1‑94 (MQ=60)
                                  GGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAAT                                                            >  SRR4302537.3124700/1‑94 (MQ=60)
                                   GTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATG                                                           >  SRR4302537.4903872/1‑94 (MQ=60)
                                     TCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCC                                                         >  SRR4302537.5449511/1‑94 (MQ=60)
                                     TCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCC                                                         >  SRR4302537.6467160/1‑94 (MQ=60)
                                     TCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCC                                                         <  SRR4302537.3008600/94‑1 (MQ=60)
                                     TCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCC                                                         <  SRR4302537.5847363/94‑1 (MQ=60)
                                       TCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCA                                                       <  SRR4302537.474614/94‑1 (MQ=60)
                                        CGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAG                                                      <  SRR4302537.3966878/94‑1 (MQ=60)
                                        CGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAG                                                      >  SRR4302537.4802404/1‑94 (MQ=60)
                                        CGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAG                                                      <  SRR4302537.2350949/94‑1 (MQ=60)
                                         GTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGATATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGC                                                     <  SRR4302537.4076338/94‑1 (MQ=60)
                                         GTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGGCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGC                                                     >  SRR4302537.6477080/1‑94 (MQ=60)
                                         GTAGTGAATTTCCCCCTGATGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGC                                                     <  SRR4302537.3466892/94‑1 (MQ=60)
                                          TAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCA                                                    >  SRR4302537.2664514/1‑94 (MQ=60)
                                           AGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCAC                                                   <  SRR4302537.2012179/94‑1 (MQ=60)
                                            GTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACT                                                  >  SRR4302537.3216231/1‑94 (MQ=60)
                                            GTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACT                                                  >  SRR4302537.57034/1‑94 (MQ=60)
                                              GAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTC                                                <  SRR4302537.3211662/94‑1 (MQ=60)
                                              GAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTC                                                >  SRR4302537.1052609/1‑94 (MQ=60)
                                               AATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCC                                               <  SRR4302537.4873635/94‑1 (MQ=60)
                                                ATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCC                                              >  SRR4302537.4831265/1‑94 (MQ=60)
                                                ATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCC                                              >  SRR4302537.5169693/1‑94 (MQ=60)
                                                ATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCC                                              >  SRR4302537.3894331/1‑94 (MQ=60)
                                                 atgCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCC                                             <  SRR4302537.3902002/91‑1 (MQ=60)
                                                  TTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCC                                            >  SRR4302537.3895997/1‑94 (MQ=60)
                                                   TCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCG                                           <  SRR4302537.5360392/94‑1 (MQ=60)
                                                   TCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCACCG                                           <  SRR4302537.1333691/94‑1 (MQ=60)
                                                     CCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGC                                         >  SRR4302537.3867215/1‑94 (MQ=60)
                                                      CCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCC                                        >  SRR4302537.1196931/1‑94 (MQ=60)
                                                       CCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCA                                       <  SRR4302537.3990929/94‑1 (MQ=60)
                                                        CTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCAT                                      >  SRR4302537.851500/1‑94 (MQ=60)
                                                         TGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATA                                     <  SRR4302537.5413085/94‑1 (MQ=60)
                                                          GCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAA                                    <  SRR4302537.2945785/94‑1 (MQ=60)
                                                           CTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAA                                   <  SRR4302537.6425683/94‑1 (MQ=60)
                                                           CTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAA                                   <  SRR4302537.3237306/94‑1 (MQ=60)
                                                           CTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAA                                   <  SRR4302537.3441308/94‑1 (MQ=60)
                                                              CGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATGA                                >  SRR4302537.3378221/1‑94 (MQ=60)
                                                                GCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAA                              <  SRR4302537.1359398/94‑1 (MQ=60)
                                                                 CGTCAGGCGCGCGGAGATCGGCTTCAGCAGCGTGGTCTTCCCGATGCCGGTTTCCCCGACAATGCCAAGCACTTCCCCCGGCCATAAATCAAAA                             >  SRR4302537.4317298/1‑94 (MQ=60)
                                                                 CGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAA                             >  SRR4302537.1367988/1‑94 (MQ=60)
                                                                 CGTAAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAA                             >  SRR4302537.2598448/1‑94 (MQ=60)
                                                                  GTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTGCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAAGCAAAAG                            <  SRR4302537.714092/94‑1 (MQ=60)
                                                                    CAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAG                          <  SRR4302537.5209635/94‑1 (MQ=60)
                                                                     AGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGA                         >  SRR4302537.1283711/1‑94 (MQ=60)
                                                                     AGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGA                         >  SRR4302537.3301814/1‑94 (MQ=60)
                                                                     AGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCAGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGA                         <  SRR4302537.3270074/94‑1 (MQ=60)
                                                                      GGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGAC                        <  SRR4302537.2054865/94‑1 (MQ=60)
                                                                        GGCGCGGAGATCGACTTCAGCGGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAAGGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACAT                      <  SRR4302537.1835449/94‑1 (MQ=60)
                                                                        CGCGCGGAGATCGTCTTCAGGAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACAT                      <  SRR4302537.4919954/94‑1 (MQ=60)
                                                                        CGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACAT                      <  SRR4302537.2789073/94‑1 (MQ=60)
                                                                        CGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACAT                      <  SRR4302537.1182231/94‑1 (MQ=60)
                                                                            CGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACATCGCT                  <  SRR4302537.6441388/94‑1 (MQ=60)
                                                                            CGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACATCGCT                  <  SRR4302537.3672778/94‑1 (MQ=60)
                                                                             GGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACATCGCTA                 >  SRR4302537.1286878/1‑94 (MQ=60)
                                                                             GGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACATCGCTA                 <  SRR4302537.749493/94‑1 (MQ=60)
                                                                             GGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACATCGCTA                 <  SRR4302537.1257003/94‑1 (MQ=60)
                                                                             GGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACATCGCTA                 >  SRR4302537.3115208/1‑94 (MQ=60)
                                                                              GAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACATCGCTAA                >  SRR4302537.2924946/1‑94 (MQ=60)
                                                                               AGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACATCGCTAAA               >  SRR4302537.4800610/1‑94 (MQ=60)
                                                                               AGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACATCGCTAAA               <  SRR4302537.983667/94‑1 (MQ=60)
                                                                               AGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACATCGCTAAA               >  SRR4302537.1904244/1‑94 (MQ=60)
                                                                               AGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACATCGCTAAA               <  SRR4302537.1325916/94‑1 (MQ=60)
                                                                               AGATCGACTTCAGCAGCGTGGGCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACATCTCTAAA               >  SRR4302537.3506134/1‑94 (MQ=60)
                                                                                GATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACATCGCTAAAG              >  SRR4302537.1084079/1‑94 (MQ=60)
                                                                                 ATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACATCGCTAAAGC             <  SRR4302537.1140177/94‑1 (MQ=60)
                                                                                 ATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACATCGCTAAAGC             >  SRR4302537.3008037/1‑94 (MQ=60)
                                                                                 ATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACATCGCTAAAGC             >  SRR4302537.3233201/1‑94 (MQ=60)
                                                                                  TCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACATCGCTAAAGCC            <  SRR4302537.5204214/94‑1 (MQ=60)
                                                                                   CGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACATCGCTCAAGCCT           >  SRR4302537.1067186/1‑94 (MQ=60)
                                                                                   CGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACATCGCTAAAGCCT           <  SRR4302537.2969439/94‑1 (MQ=60)
                                                                                   CGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACATCGCTAAAGCCT           <  SRR4302537.4774791/94‑1 (MQ=60)
                                                                                    GACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACATCGCTAAAGCCTT          <  SRR4302537.131196/94‑1 (MQ=60)
                                                                                    GACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACATCGCTAAAGCCTT          <  SRR4302537.894364/94‑1 (MQ=60)
                                                                                     ACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACATCGCTAAAGCCTTT         <  SRR4302537.3441162/94‑1 (MQ=60)
                                                                                      CTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACATCGCTAAAGCCTTTG        >  SRR4302537.4437818/1‑94 (MQ=60)
                                                                                      CTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACATCGCTAAAGCCTTTG        <  SRR4302537.4628857/94‑1 (MQ=60)
                                                                                        TCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACATCGCTAAAGCCTTTGCC      <  SRR4302537.4342093/94‑1 (MQ=60)
                                                                                          AGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAATAGACATCGCTAAAGCCTTTGCCCG    <  SRR4302537.331106/94‑1 (MQ=60)
                                                                                          AGCAGCGTGGGCTTCCCGGACCCGGATCCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACATCGCTAAAGCCTTTGCCCG    <  SRR4302537.4690879/94‑1 (MQ=60)
                                                                                           GCAGCGTGGTCTTCCCGGAGCCGCATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACATCGCTAAAGCCTTTGCCCGG   <  SRR4302537.1020434/94‑1 (MQ=60)
                                                                                            CAGCGTGGTCTGCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACATCGCTAAAGCCTTTGCCCGGC  <  SRR4302537.6471635/94‑1 (MQ=60)
                                                                                             |                                                                                            
CGGCGGTCGGCCTCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCTGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACATCGCTAAAGCCTTTGCCCGGC  >  NC_000913/4316110‑4316295

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑