Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	882	254.4	12957588	98.0%	12698436	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,375,270	T→C	T11A (ACA→GCA)	blc ←	outer membrane lipoprotein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,375,270	0	T	C	96.4%	892.2 / 15.7	275	T11A (ACA→GCA)	blc	outer membrane lipoprotein
Reads supporting (aligned to +/- strand): ref base T (4/6); new base C (104/161); total (108/167)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.66e-01

Only 100 of 280 total aligned reads displayed.

GAGATAACGTTTGGCGTCGAAATTATTTACTACGGTCACGCCACGCGGCGGCGTAGGAGAACTGCAGGCAACGACCAGAAATGCAGCTGTCGCTGCGGCAACGAGAGGGAGCAGGCGCATAAATGTTTCCTTACTGGTTTTTTTCTAAGTGTAGATGACAGCAAGGAAAAGCGGAGAAA  >  NC_000913/4375181‑4375359

gagaTAACGTTTGGCGTCGAAATTATTTACTACGGTCACGCCACGCGGCGGCGTAGGAGAACTGCAGGCAACGACCAGAAATGCAGCTGCCGc                                                                                        >  1:5134709/1‑93 (MQ=255)

gagaTAACGTTGGGCGTCGAAATTATTTACTACGGTCACGCCACGCGGCGGCGTAGGAGAACTGCAGGCAACGACCAGAAATGCAGCTGCCGc                                                                                        <  2:1687846/93‑1 (MQ=255)

 agaTAACGTTTGGCGTCGAAATTATTTACTACGGTCACGCCACGCGGCGGCGTAGGAGAACTGCAGGCAACGACCAGAAATGCAGCTGCCGCt                                                                                       <  2:2665783/93‑1 (MQ=255)

 agaTAACGTTTGGCGTCGAAATTATTTACTACGGTCACGCCACGCGGCGGCGTAGGAGAACTGCAGGCAACGACCAGAAATGCAGCTGCCGCt                                                                                       >  1:1145129/1‑93 (MQ=255)

 agaTAACGTTTGGCGTCGAAATTATTTACTACGGTCACGCCACGCGGCGGCGTAGGAGAACTGCAGGCAACGACCAGAAATGCAGCTGCCGCt                                                                                       <  1:5974449/93‑1 (MQ=255)

 agaTAACGTTTGGCGTCGAAATTATTTACTACGGTCACGCCACGCGGCGGCGTAGGAGAACTGCAGGCAACGACCAGAAATGCAGCTGCCGCt                                                                                       <  2:2492084/93‑1 (MQ=255)

 agaTAACGCTTGGCGTCGAAATTATTTACTACGGTCACGCCACGCGGCGGCGTAGGAGAACTGCAGGCAACGACCAGAAATGCAGCTGCCGCt                                                                                       >  2:6368473/1‑93 (MQ=255)

   aTAACGTTTGGCGTCGAAATTATTTACTACGGTCACGCCACGCGGCGGCGTAGGAGAACTGCAGGCAACGACCAGAAATGCAGCTGCCGCTGc                                                                                     >  1:3213276/1‑93 (MQ=255)

    tAACGTTTGGCGTCGAAATTATTTACTACGGTCACGCCACGCGGCGGCGTAGGAGAACTGCAGGCAACGACCAGAAATGCAGCTGCCGCTGCg                                                                                    >  2:2921401/1‑93 (MQ=255)

    tAACGTTTGGCGTCGAAATTATTTACTACGGTCACGCCACGCGGCGGCGTAGGAGAACTGCAGGCAACGACCAGAAATGCAGCTGCCGCTGCg                                                                                    >  1:2139468/1‑93 (MQ=255)

     aaCGTTTGGCGTCGAAATTATTTACTACGGTCACGCCACGCGGCGGCGTAGGAGAACTGCAGGCAACGACCAGAAATGCAGCTGTCGCTGCgg                                                                                   >  2:1463445/1‑93 (MQ=255)

     aaCGTTTGGCGTCGAAATTATTTACTACGGTCACGCCACGCGGCGGCGTAGGAGAACTGCAGGCAACGACCAGAAATGCAGCTGCCGCTGCgg                                                                                   <  1:241588/93‑1 (MQ=255)

     aaCGTTTGGCGTCGAAATTATTTACTACGGTCACGCCACGCGGCGGCGTAGGAGAACTGCAGGCAACGACCAGAAATGCAGCTGCCGCTGCgg                                                                                   >  2:2251730/1‑93 (MQ=255)

      aCGTTTGGCGTCGAAATTATTTACTACGGTCACGCCACGCGGCGGCGTAGGAGAACTGCAGGCAACGACCAGAAATGCAGCTGCCGCTGCGGc                                                                                  <  1:1808063/93‑1 (MQ=255)

       cGTTTGGCGTCGAAATTATTTACTACGGTCACGCCACGCGGCGGCGTAGGAGAACTGCAGGCAACGACCAGAAATGCAGCTGCCGCTGCGGCa                                                                                 >  1:5059658/1‑93 (MQ=255)

       cGTTTGGCGTCGAAATTATTTACTACGGTCACGCCACGCGGCGGCGTAGGAGAACTGCAGGCAACGACCAGAAATGCAGCTGCCGCTGCGGCa                                                                                 >  2:3311592/1‑93 (MQ=255)

        gTTTGGCGTCGAAATTATTTACTACGGTCACGCCACGCGGCGGCGTAGGAGAACTGCAGGCAACGACCAGAAATGCAGCTGCCGCTGCGGCaa                                                                                <  1:1008572/93‑1 (MQ=255)

        gTTTGGCGTCGAAATTATTTACTACGGTCACGCCACGCGGCGGCGTAGGAGAACTGCAGGCAACGACCAGAAATGCAGCTGCCGCTGCGGCaa                                                                                >  1:3374394/1‑93 (MQ=255)

        gTTTGGCGTCGAAATTATTTACTACGGTCACGCCACGCGGCGGCGTAGGAGAACTGCAGGCAACGACCAGAAATGCAGCTGCCGCTGCGGCaa                                                                                <  2:5385368/93‑1 (MQ=255)

         tttGGCGTCGAAATTATTTACTACGGTCACGCCACGCGGCGGCGTAGGAGAACTGCAGGCAACGACCAGAAATGCAGCTGCCGCTGCGGCAAc                                                                               >  1:5308906/1‑93 (MQ=255)

         tttGGCGTCGAAATTATTTACTACGGTCACGCCACGCGGCGGCGTAGGAGAACTGCAGGCAACGACCAGAAATGCAGCTGCCGCTGCGGCAAc                                                                               <  1:3755720/93‑1 (MQ=255)

           tGGCGTCGAAATTATTTACTACGGTCACGCCACGCGGCGGCGTAGGAGAACTGCAGGCAACGACCAGAAATGCAGCTGCCGCTGCGGCAACga                                                                             <  1:3007060/93‑1 (MQ=255)

           tGGCGTCGAAATTATTTACTACGGTCACGCCACGCGGCGGCGTAGGAGAACTGCAGGCAACGACCAGAAATGCAGCTGCCGCTGCGGCAACga                                                                             >  2:1414758/1‑93 (MQ=255)

            ggCGTCGAAATTATTTACTACGGTCACGCCACGCGGCGGCGTAGGAGAACTGCAGGCAACGACCAGAAATGCAGCTGCCGCTGCGGCAACgag                                                                            >  2:263478/1‑93 (MQ=255)

            ggCGTCGAAATTATTTACTACGGTCACGCCACGCGGCGGCGTAGGAGAACTGCAGGCAACGACCAGAAATGCAGCTGCCGCTGCGGCAACgag                                                                            >  2:341389/1‑93 (MQ=255)

            ggCGTCGAAATTATTTACTACGGTCACGCCACGCGGCGGCGTAGGAGAACTGCAGGCAACGACCAGAAATGCAGCTGCCGCTGCGGCAACgag                                                                            >  1:3668943/1‑93 (MQ=255)

              cGTCGAAATTATTTACTACGGTCACGCCACGCGGCGGCGTAGGAGAACTGCAGGCAACGACCAGAAATGCAGCTGCCGCTGCGGCAACGagag                                                                          <  2:5678384/93‑1 (MQ=255)

               gTCGAAATTATTTACTACGGTCACGCCACGCGGCGGCGTAGGAGAACTGCAGGCAACGACCAGAAATGCAGCTGCTGCTGCGGCAACGAGAgg                                                                         <  2:6339423/93‑1 (MQ=255)

                tCGAAATTATTTACTACGGTCACGCCACGCGGCGGCGTAGGAGAACTGCAGGCAACGACCAGAAATGCAGCTGCCGCTGCGGCAACGAGAggg                                                                        <  1:3898319/93‑1 (MQ=255)

                tCGAAATTATTTACTACGGTCACGCCACGCGGCGGCGTAGGAGAACTGCAGGCAACGACCAGAAATGCAGCTGCCGCTGCGGCAACGAGAggg                                                                        <  2:4188421/93‑1 (MQ=255)

                tCGAAATTATTTACTACGGTCACGCCACGCGGCGGCGTAGGAGAACTGCAGGCAACGACCAGAAATGCAGCTGCCGCTGCGGCAACGAGAggg                                                                        <  1:1722994/93‑1 (MQ=255)

                 cGAAATTATTTACTACGGTCACGCCACGCGGCGGCGTAGGAGAACTGCAGGCAACGACCAGAAATGCAGCTGCCGCTGCGGCAACGAGAGGGa                                                                       >  1:4509627/1‑93 (MQ=255)

                  gAAATTATTTACTACGGTCCCGCCACGCGGCGGCGTAGGAGAACTGCAGGCAACGACCAGAAATGCAGCTGCCGCTGCGGCAACGAGAGGGAg                                                                      <  2:3127223/93‑1 (MQ=255)

                  gAAATTATTTACTACGGTCACGCCACGCGGCGGCGTAGGAGAACTGCAGGCAACGACCAGAAATGCAGCTGCCGCTGCGGCAACGAGAGGGAg                                                                      >  1:4250369/1‑93 (MQ=255)

                   aaaTTATTTACTACGGTCACGCCACGCGGCGGCGTAGGAGAACTGCAGGCAACGACCAGAAATGCAGCTGCCGCTGCGGCAACGAGAGGGAGc                                                                     >  1:5424861/1‑93 (MQ=255)

                   aaaTTATTTACTACGGTCACCCCACGCGGCGGCGTAGGAGAACTGCAGGCAACGACCAGAAATGCAGCTGCCGCTGCGGCAACGAGAGGGAGc                                                                     <  2:4688097/93‑1 (MQ=255)

                     attattTACTACGGTCACGCCACGCGGCGGCGTAGGAGAACTGCAGGCAACGACCAGAAATGCAGCTGCCGCTGCGGCAACGAGAGGGAGCAg                                                                   <  2:5960207/93‑1 (MQ=255)

                     attattTACTACGGTCACGCCACGCGGCGGCGTAGGAGAACTGCAGGCAACGACCAGAAATGCAGCTGCCGCTGCGGCAACGAGAGGGAGCAg                                                                   <  2:4477410/93‑1 (MQ=255)

                      ttattTACTACGGTCACGCCACGCGGCGGCGTAGGAGAACTGCAGGCAACGACCAGAAATGCAGCTGCCGCTGCGGCAACGAGAGGGAGCAgg                                                                  <  2:4839173/93‑1 (MQ=255)

                         tttACTACGGTCACGCCACGCGGCGGCGTAGGAGAACTGCAGGCAACGACCAGAAATGCAGCTGCCGCTGCGGCAACGAGAGGGAGCAGgcgc                                                               <  2:364119/93‑1 (MQ=255)

                         tttACTACGGTCACGCCACGCGGCGGCGTAGGAGAACTGCAGGCAACGACCAGAAATGCAGCTGCCGCTGCGGCAACGAGAGGGAGCAGgcgc                                                               >  1:1022391/1‑93 (MQ=255)

                          ttACTACGGTCACGCCACGCGGCGGCGTAGGAGAACTGCAGGCAACGACCAGAAATGCAGCTGCCGCTGCGGCAACGAGAGGGAGCAGGCGCa                                                              >  2:679164/1‑93 (MQ=255)

                          ttACTACGGTCACGCCACGCGGCGGCGTAGGAGAACTGCAGGCAACGACCAGAAATGCAGCTGCCGCTGCGGCAACGAGAGGGAGCAGGCGCa                                                              <  2:3828450/93‑1 (MQ=255)

                              tacGGTCACGCCACGCGGCGGCGTATGAGAACTGCAGGCAACGACCAGAAATGCAGCTGCCGCTGCGGCAACGAGAGGGAGCAGGCGCATaat                                                          >  1:3434223/1‑92 (MQ=255)

                              tacGGTCACGCCACGCGGCGGCGTAGGAGAACTGCAGGCAACGACCAGAAATGCAGCTGCCGCTGCGGCAACGAGAGGGAGCAGGCGCATaaa                                                          >  1:4707460/1‑93 (MQ=255)

                              tacGGTCACGCCACGCGGCGGCGTAGGAGAACTGCAGGCAACGACCAGAAATGCAGCTGCCGCTGCGGAAACGAGAGGGAGCAGGCGCGTaaa                                                          >  2:3602132/1‑93 (MQ=255)

                                cGGTCACGCCACGCGGCGGCGTAGGAGAACTGCAGGCAACGACCAGAAATGCAGCTGCCGCTGCGGCAACGAGAGGGAGCAGGCGCATAAATg                                                        >  1:5548600/1‑93 (MQ=255)

                                cGGTCACGCCACGCGGCGGCGTAGGAGAACTGCAGGCAACGACCAGAAATGCAGCTGCCGCTGCGGCAACGAGAGGGAGCAGGCGCATAAATg                                                        <  2:3740835/93‑1 (MQ=255)

                                 ggTCACGCCACGCGGCGGCGTAGGAGAACTGCAGGCAACGACCAGAAATGCAGCTGCCGCTGCGGCAACGAGAGGGAGCAGGCGCATAAATGt                                                       <  1:5902935/93‑1 (MQ=255)

                                   tCACGCCACGCGGCGGCGTAGGAGAACTGCAGGCAACGACCAGAAATGCAGCTGCCGCTGCGGCAACGAGAGGGAGCAGGCGCATAAATGttt                                                     <  1:6346688/93‑1 (MQ=255)

                                     acgccacgcGGCGGCGTAGGAGAACTGCAGGCAACGACCAGAAATGCAGCTGCCGCTGCGGCAACGAGAGGGAGCAGGCGCATAAATGTTTcc                                                   >  2:3219004/1‑93 (MQ=255)

                                          acgcGGCGGCGTAGGAGAACTGCAGGCAACGACCAGAAATGCAGCTGCCGCTGCGGCAACGAGAGGGAGCAGGCGCATAAATGTTTCCTTACt                                              <  1:5590832/93‑1 (MQ=255)

                                           cgcgGCGGCGTAGGAGAACTGCAGGCAACGACCAGAAATGCAGCTGCCGCTGCGGCAACGAGAGGGAGCAGGCGCATAAATGTTTCCTTACTg                                             >  1:1249400/1‑93 (MQ=255)

                                            gcggcggcgTAGGAGAACTGCAGGCAACGACCAGAAATGCAGCTGCCGCTGCGGCAACGAGAGGGAGCAGGCGCATAAATGTTTCCTTACTgg                                            <  1:3624062/93‑1 (MQ=255)

                                             cggcggcgTAGGAGAACTGCAGGCAACGACCAGAAATGCAGCTGCCGCTGCGGCAACGAGAGGGAGCAGGCGCATAAATGTTTCCTTACTGGt                                           >  2:2112333/1‑93 (MQ=255)

                                             cggcggcgTAGGAGAACTGCAGGCAACGACCAGAAATGCAGCTGCCGCTGCGGCAACGAGAGGGAGCAGGCGCATAAATGTTTCCTTACTGGt                                           >  2:2218342/1‑93 (MQ=255)

                                              ggcggcgTAGGAGAACTGCAGGCAACGACCAGAAATGCAGCTGCCGCTGCGGCAACGAGAGGGAGCAGGCGCATAAATGTTTCCTTACTGGtt                                          <  1:196796/93‑1 (MQ=255)

                                               gcggcgTAGGAGAACTGCAGGCAACGACCAGAAATGCAGCTGCCGCTGCGGCAACGAGAGGGAGCAGGCGCATAAATGTTTCCTTACTGGttt                                         <  1:331516/93‑1 (MQ=255)

                                               gcggcgTAGGAGAACTGCAGGCAACGACCAGAAATGCAGCTGCCGCTGCGGCAACGAGAGGGAGCAGGCGCATAAATGTTTCCTTACTGGttt                                         <  2:2711939/93‑1 (MQ=255)

                                                 ggcgTAGGAGAACTGCAGGCAACGACCAGAAATGCAGCTGCCGCTGCGGCAACGAGAGGGAGCAGGCGCATAAATGTTTCCTTACTGGttttt                                       <  1:602444/93‑1 (MQ=255)

                                                  gcgTAGGAGAACTGCAGGCAACGACCAGAAATGCAGCTGCCGCTGCGGCAACGAGAGGGAGCAGGCGCATAAATGTTTCCTTACTGGtttttt                                      >  1:955977/1‑93 (MQ=255)

                                                  gcgTAGGAGAACTGCAGGCAACGACCAGAAATGCAGCTGCCGCTGCGGCAACGAGAGGGAGCAGGCGCATAAATGTTTCCTTACTGGtttttt                                      <  2:2860323/93‑1 (MQ=255)

                                                  gcgTAGGAGAACTGCAGGCAACGACCAGAAATGCAGCTGCCGCTGCGGCAACGAGAGGGAGCAGGCGCATAAATGTTTCCTTACTGGtttttt                                      >  2:6232819/1‑93 (MQ=255)

                                                    gTAGGAGAACTGCAGGCAACGACCAGAAATGCAGCTGTCGCTGCGGCAACGAGAGGGAGCAGGCGCATAAATGTTTCCTTACTGGTTTTTTTc                                    <  2:4898696/93‑1 (MQ=255)

                                                    gTAGGAGAACTGCAGGCAACGACCAGAAATGCAGCTGCCGCTGCGGCAACGAGAGGGAGCAGGCGCATAAATGTTTCCTTACTGGTTTTTTTc                                    >  1:5630418/1‑93 (MQ=255)

                                                     tAGGAGAACTGCAGGCAACGACCAGAAATGCAGCTGTCGCTGCGGCAACGAGAGGGAGCAGGCGCATAAATGTTTCCTTACTGGTTTTTTTCt                                   <  2:755603/93‑1 (MQ=255)

                                                     tAGGAGAACTGCAGGCAACGACCAGAAATGCAGCTGCCGCTGCGGCAACGAGAGGGAGCAGGCGCATAAATGTTTCCTTACTGGTTTTTTTCt                                   >  1:1646695/1‑93 (MQ=255)

                                                     tAGGAGAACTGCAGGCAACGACCAGAAATGCAGCTGCCGCTGAGGCAACGAGAGGGAGCAGGCGCATAAATGTTTCCTTACTGGTTTTTTTCt                                   >  1:3466289/1‑93 (MQ=255)

                                                       ggAGAACTGCAGGCAACGACCAGAAATGCAGCTGCCGCTGCGGCAACGAGAGGGAGCAGGCGCATAAATGTTTCCTTACTGGTTTTTTTCTaa                                 <  1:516663/93‑1 (MQ=255)

                                                       ggAGAACTGCAGGCAACGACCAGAAATGCAGCTGCCGCTGCGGCAACGAGAGGGAGCAGGCGCATAAATGTTTCCTTACTGGTTTTTTTCTaa                                 >  1:1508186/1‑93 (MQ=255)

                                                       ggAGAACTGCAGGCAACGACCAGAAATGCAGCTGCCGCTGCGGCAACGAGAGGGAGCAGGCGCATAAATGTTTCCTTACTGGTTTTTTTCTaa                                 <  2:4079233/93‑1 (MQ=255)

                                                        gagaACTGCAGGCAACGACCAGAAATGCAGCTGCCGCTGCGGCAACGAGAGGGAGCAGGCGCATAAATGTTTCCTTACTGGTTTTTTTCTAAg                                <  2:1078694/93‑1 (MQ=255)

                                                        gagaACTGCAGGCAACGACCAGAAATGCAGCTGCCGCTGCGGCAACGAGAGGGAGCAGGCGCATAAATGTTTCCTTACTGGTTTTTTTCTAAg                                >  2:4395268/1‑93 (MQ=255)

                                                         agaACTGCAGGCAACGACCAGAAATGCAGCTGCCGCTGCGGCAACGAGAGGGAGCAGGCGCATAAATGTTTCCTTACTGGTTTTTTTCTAAgt                               <  1:2613601/93‑1 (MQ=255)

                                                         agaACTGCAGGCAACGACCAGAAATGCAGCTGCCGCTGCGGCAACGAGAGGGAGCAGGCGCATAAATGTTTCCTTACTGGTTTTTTTCTAAgt                               <  1:4961390/93‑1 (MQ=255)

                                                           aaCTGCAGGCAACGACCAGAAATGCAGCTGCCGCTGCGGCAACGAGAGGGAGCAGGCGCATAAATGTTTCCTTACTGGTTTTTTTCTAAgtgt                             <  1:4161615/93‑1 (MQ=255)

                                                            aCTGCAGGCAACGACCAGAAATGCAGCTGCCGCTGCGGCAACGAGAGGGAGCAGGCGCATAAATGTTTCCTTACTGGTTTTTTTCTAAGTGTa                            <  2:2557144/93‑1 (MQ=255)

                                                              tGCAGGCAACGACCAGAAATGCAGCTGCCGCTGCGGCAACGAGAGGGAGCAGGCGCATAAATGTTTCCTTACTGGTTTTTTTCTAAGTGTAGa                          <  2:5022657/93‑1 (MQ=255)

                                                              tGCAGGCAACGACCAGAAATGCAGCTGCCGCTGCGGCAACGAGAGGGAGCAGGCGCATAAATGTTTCCTTACTGGTTTTTTTCTAAGTGTAGa                          >  2:4137210/1‑93 (MQ=255)

                                                                 aGGCAACGACCAGAAATGCAGCTGCCGCTGCGGCAACGAGAGGGAGCAGGCGCATAAATGTTTCCTTACTGGTTTTTTTCTAAGTGTAGATGa                       >  1:2226581/1‑93 (MQ=255)

                                                                 aGGCAACGACCAGAAATGCAGCTGCCGCTGCGGCAACGAGAGGGAGCAGGCGCATAAATGTTTCCTTACTGGTTTTTTTCTAAGTGTAGATGa                       <  1:5810093/93‑1 (MQ=255)

                                                                 aGGCAACGACCAGAAATGCAGCTGCCGCTGCGGCAACGAGAGGGAGCAGGCGCATAAATGTTTCCTTACTGGTTTTTTTCTAAGTGTAGATGa                       <  2:5584847/93‑1 (MQ=255)

                                                                  ggCAACGACCAGAAATGCAGCTGCCGCTGCGGCAACGAGAGGGAGCAGGCGCATAAATGTTTCCTTACTGGTTTTTTTCTAAGTGTAGATGAc                      <  2:1361369/93‑1 (MQ=255)

                                                                  ggCAACGACCAGAAATGCAGCTGCCGCTACGGCAACGAGAGGGAGCAGGCGCATAAATGTTTCCTTACTGGTTTTTTTCTAAGTGTAGATGAc                      <  1:6219646/93‑1 (MQ=255)

                                                                       cGACCAGAAATGCAGCTGCCGCTGCGGCAACGAGAGGGAGCAGGCGCATAAATGTTTCCTTACTGGTTTTTTTCTAAGTGTAGATGACAGCaa                 <  1:4375396/93‑1 (MQ=255)

                                                                       cGACCAGAAATGCAGCTGCCGCTGCGGCAACGAGAGGGAGCAGGCGCATAAATGTTTCCTTACTGGTTTTTTTCTAAGTGTAGATGACAGCaa                 <  1:742555/93‑1 (MQ=255)

                                                                        gACCAGAAATGCAGCTGCCGCTGCGGCAACGAGAGGGAGCAGGCGCATAAATGTTTCCTTACTGGTTTTTTTCTAAGTGTAGATGACAGCAAg                <  2:4309082/93‑1 (MQ=255)

                                                                         aCCAGAAATGCAGCTGCCGCTGCGGCAACGAGAGGGAGCAGGCGCATAAATGTTTCCTTACTGGTTTTTTTCTAAGTGTAGATGACAGCAAgg               <  2:847156/93‑1 (MQ=255)

                                                                         aCCAGAAATGCAGCTGCCGCTGCGGCAACGAGAGGGAGCAGGCGCATAAATGTTTCCTTACTGGTTTTTTTCTAAGTGTAGATGACAGCAAgg               <  2:1241547/93‑1 (MQ=255)

                                                                         aCCAGAAATGCAGCTGCCGCTGCGGCAACGAGAGGGAGCAGGCGCATAAATGTTTCCTTACTGGTTTTTTTCTAAGTGTAGATGACAGCAAgg               <  1:915256/93‑1 (MQ=255)

                                                                             gAAATGCAGCTGCCGCTGCGGCAACGAGAGGGAGCAGGCGCATAAATGTTTCCTTACTGGTTTTTTTCTAAGTGTAGATGACAGCAAGGaaaa           >  1:3040054/1‑93 (MQ=255)

                                                                              aaaTGCAGCTGCCGCTGCGGCAACGAGAGGGAGCAGGCGCATAAATGTTTCCTTACTGGTTTTTTTCTAAGTGTAGATGACAGCAAGGAAAAg          <  1:5750706/93‑1 (MQ=255)

                                                                              aaaTGCAGCTGCCGCTGCGGCAACGAGAGGGAGCAGGCGCATAAATGTTTCCTTACTGGTTTTTTTCTAAGTGTAGATGACAGCAAGGAAAAg          >  1:4634463/1‑93 (MQ=255)

                                                                                aTGCAGCTGCCGCTGCGGCAACGAGAGGGAGCAGGCGCATAAATGTTTCCTTACTGGTTTTTTTCTAAGTGTAGATGACAGCAAGGAAAAGCg        <  2:3459033/93‑1 (MQ=255)

                                                                                aTGCAGCTGCCGCTGCGGCAACGAGAGGGAGCAGGCGCATAAATGTTTCCTTACTGGTTTTTTTCTAAGTGTAGATGACAGCAAGGAAAAGCg        >  2:1888391/1‑93 (MQ=255)

                                                                                  gCAGCTNCCGCTGCGGCAACGAGAGGGAGCAGGCGCATAAATGTTTCCTTACTGGTTTTTTTCTAAGTGTAGATGACAGCAAGGAAAAGCGga      <  1:1449969/93‑1 (MQ=255)

                                                                                   cAGCTGCCGCTGCGGCAACGAGAGGGAGCAGGCGCATAAATGTTTCCTTACTGGTTTTTTTCTAAGTGTAGATGACAGCAAGGAAAAGCGgag     <  2:5879903/93‑1 (MQ=255)

                                                                                   cAGCTGCCGCTGCGGCAACGAGAGGGAGCAGGCGCATAAATGTTTCCTTACTGGTTTTTTTCTAAGTGTAGATGACAGCAAGGAAAAGCGgag     <  1:5225824/93‑1 (MQ=255)

                                                                                   cAGCTGCCGCTGCGGCAACGAGAGGGAGCAGGCGCATAAATGTTTCCTTACTGGTTTTTTTCTAAGTGTAGATGACAGCAAGGAAAAGCGgag     >  1:2018322/1‑93 (MQ=255)

                                                                                      cTGCCGCTGCGGCAACGAGAGGGAGCAGGCGCATAAATGTTTCCTTACTGGTTTTTTTCTAAGTGTAGATGACAGCAAGGAAAAGCGGAGaaa  >  2:29846/1‑93 (MQ=255)

GAGATAACGTTTGGCGTCGAAATTATTTACTACGGTCACGCCACGCGGCGGCGTAGGAGAACTGCAGGCAACGACCAGAAATGCAGCTGTCGCTGCGGCAACGAGAGGGAGCAGGCGCATAAATGTTTCCTTACTGGTTTTTTTCTAAGTGTAGATGACAGCAAGGAAAAGCGGAGAAA  >  NC_000913/4375181‑4375359

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

TACCGAGATAACGTTTGGCGTCGAAATTATTTACTACGGTCACGCCACGCGGCGGCGTAGGAGAACTGCAGGCAACGACCAGAAATGCAGCTGTCGCTGCGGCAACGAGAGGGAGCAGGCGCATAAATGTTTCCTTACTGGTTTTTTTCTAAGTGTAGATGACAGCAAGGAAAAGCGGAGAAAAGGT  >  NC_000913/4375177‑4375363

gacaGAGATAACGTTTGGCGTCGAAATTATTTACTACGGTCACGCCACGCGGCTGCGTAGGAGAACTGCAGGCAACGACCAGAAATGCAGCTGC                                                                                               <  SRR4302537.185037/90‑1 (MQ=60)

 ACCGAGATAACGTTTGGCGTCGAAATTATTTACTACGGTCACGCCACGCGGCGGCGTAGGAGAACTGCAGGCAACGACCAGAAATGCAGCTGCC                                                                                              >  SRR4302537.3527410/1‑94 (MQ=60)

 ACCGAGATAACGTTTGGCGTCGAAATTATTTACTACGGTCACGCCACGCGGCGGCGTAGGAGAACTGCAGGCAACGACCAGAAATGCAGCTGCC                                                                                              <  SRR4302537.3045982/94‑1 (MQ=60)

 ACCGAGATAACGTTTGGCGTCGAAATTATTTACTACGGTCACGCCACGCGGCGGCGTAGGAGAACTGCAGGCAACGACCAGAAATGCAGCTGCC                                                                                              <  SRR4302537.1358504/94‑1 (MQ=60)

   CGAGATAACGTTTGGCGTCGAAATTATTTACTGCGGTCACGCCACGCGGCGGCGTAGGAGAACTGCAGGCAACGACCAGAAATGCAGCTGCCGC                                                                                            >  SRR4302537.4338739/1‑94 (MQ=60)

   CGAGATAACGTTTGGCGTCGAAATTATTTACTACGGTCACGCCACGCGGCGGCGTAGGAGAACTGCAGGCAACGACCAGAAATGCAGCTGCCGC                                                                                            >  SRR4302537.1865692/1‑94 (MQ=60)

   CGAGATAACGTTTGGCGTCGAAATTATTTACTACGGTCACGCCACGCGGCGGCGTAGGAGAACTGCAGGCAACGACCAGAAATGCAGCTGCCGC                                                                                            >  SRR4302537.5170089/1‑94 (MQ=60)

    GAGATAACGTTTGGCGTCGAAATTATTTACTACGGTCACGCCACGCGGCGGCGTAGGAGAACTGCAGGCAACGACCAGAAATGCAGCTGCCGCT                                                                                           <  SRR4302537.5593418/94‑1 (MQ=60)

    GAGATAACGTTTGGCGTCGAAATTATTTACTACGGTCACGCCACGCGGCGGCGTAGGAGAACTGCAGGCAACGACCAGAAATGCAGCTGCCGCT                                                                                           >  SRR4302537.2242771/1‑94 (MQ=60)

    GAGATAACGTTTGGCGTCGAAATTATTTACTACGGTCACGCCACGCGGCGGCGTAGGAGAACTGCAGGCAACGACCAGAAATGCAGCTGCCGCT                                                                                           >  SRR4302537.5595075/1‑94 (MQ=60)

    GAGATAACGTTTGGCGTCGAAATTATTTACTACGGTCACGCCACGCGGCGGCGTAGGAGAACTGCAGGCAACGACCAGAAATGCAGCTGCCGCT                                                                                           >  SRR4302537.1153092/1‑94 (MQ=60)

    GAGATAACGTTTGGCGTCGAAATTATTTACTACGGTCACGCCACGCGGCGGCGTAGGAGAACTGCAGGCAACGACCAGAAATGCAGCTGCCGCT                                                                                           >  SRR4302537.4371784/1‑94 (MQ=60)

     AGATAACGTTTGGCGTCGAAATTATTTACTACGGTCACGCCACGCGGCGGCGTAGGAGAACTGCAGGCAACGACCAGAAATGCAGCTGTCGCTG                                                                                          <  SRR4302537.1732011/94‑1 (MQ=60)

     AGATAACGTTTGGCGTCGAAATTATTTACTACGGTCACGCCACGCGGCGGCGTAGGAGAACTGCAGGCAACGACCAGAAATGCAGCTGCCGCTG                                                                                          >  SRR4302537.3137547/1‑94 (MQ=60)

     AGATAACGTTTGGCGTCGAAATTATTTACTACGGTCACGCCACGCGGCGGCGTAGGAGAACTGCAGGCAACGACCAGAAATGCAGCTGCCGCTG                                                                                          <  SRR4302537.6015461/94‑1 (MQ=60)

      GATAACGTTTGGCGTCGAAATTATTTACTACGGTCACGCCACGCGGCGGCGTAGGAGAACTGCAGGCAACGACCAGAAATGCAGCTGCCGCTGC                                                                                         >  SRR4302537.3235887/1‑94 (MQ=60)

      GATAACGTTTGGCGTCGAAATTATTTACTACGGTCACGCCACGCGGCGGCGTAGGAGAACTGCAGGCAACGACCAGAAATGCAGCTGCCGCTGC                                                                                         >  SRR4302537.650611/1‑94 (MQ=60)

      GATAACGTTTGGCGTCGAAATTATTTACTACGGTCACGCCACGCGGCGGCGTAGGAGAACTGCAGGCAACGACCAGAAATGCAGCTGCCGCTGC                                                                                         <  SRR4302537.5941264/94‑1 (MQ=60)

       ATAACGTTTGGCGTCGAAATTATTTACTACGGTCACGCCACGCGGCGGCGTAGGAGAACTGCAGGCAACGACCAGAAATGCAGCTGCCGCTGCG                                                                                        >  SRR4302537.2520390/1‑94 (MQ=60)

       ATAACGTTTGGCGTCGAAATTATTTACTACGGTCACGCCACGCGGCGGCGTAGGAGAACTGCAGGCAACGACCAGAAATGCAGCTGCCGCTGCG                                                                                        >  SRR4302537.2154528/1‑94 (MQ=60)

         AACGTTTGGCGTCGAAATTATTTACTACGGTCACGCCACGCGGCGGCGTAGGAGAACTGCAGGCAACGACCAGAAATGCAGCTGCCGCTGCGGC                                                                                      <  SRR4302537.243300/94‑1 (MQ=60)

          ACGTTTGGCGTCGAAATTATTTACTACGGTCACGCCACGCGGCGGCGTAGGAGAACTGCAGGCAACGACCAGAAATGCAGCTGCCGCTGCGGCA                                                                                     >  SRR4302537.5094490/1‑94 (MQ=60)

          ACGTTTGGCGTCGAAATTATTTACTACGGTCACGCCACGCGGCGGCGTAGGAGAACTGCAGGCAACGACCAGAAATGCAGCTGCCGCTGCGGCA                                                                                     <  SRR4302537.1820678/94‑1 (MQ=60)

           CGTTTGGCGTCGAAATTATTTACTACGGTCACGCCACGCGGCGGCGTAGGAGAACTGCAGGCAACGACCAGAAATGCAGCTGCCGCTGCGGCAA                                                                                    >  SRR4302537.3398307/1‑94 (MQ=60)

            GTTTGGCGTCGAAATTATTTACTACGGTCACGCCACGCGGCGGCGTAGGAGAACTGCAGGCAACGACCAGAAATGCAGCTGCCGCTGCGGCAAC                                                                                   <  SRR4302537.1015528/94‑1 (MQ=60)

            GTTTGGCGTCGAAATTATTTACTACGGTCACGCCACGCGGCGGCGTAGGAGAACTGCAGGCAACGACCAGAAATGCAGCTGCCGCTGCGGCAAC                                                                                   >  SRR4302537.5345380/1‑94 (MQ=60)

            GTTTGGCGTCGAAATTATTTACTACGGTCACGCCACGCGGCGGCGTAGGAGAACTGCAGGCAACGACCAGAAATGCAGCTGCCGCTGCGGCAAC                                                                                   <  SRR4302537.5945754/94‑1 (MQ=60)

             TTTGGCGTCGAAATTATTTACTACGGTCACGCCACGCGGCGGCGTAGGAGAACTGCAGGCAACGACCAGAAATGCAGCTGCCGCTGCGGCAACG                                                                                  <  SRR4302537.3782092/94‑1 (MQ=60)

              TTGGCGTCGAAATTATTTACTACGGTCACGCCACGCGGCGGCGTAGGAGAACTGCAGGCAACGACCAGAAATGCAGCTGTCGCTGCGGCAACGA                                                                                 <  SRR4302537.6248854/94‑1 (MQ=60)

              TTGGCGTCGAAATTATTTACTACGGTCACGCCACGCGGCGGCGTAGGAGAACTGCAGGCAACGACCAGAAATGCAGCTGCCGCTGCGGCAACGA                                                                                 <  SRR4302537.3809167/94‑1 (MQ=60)

               TGGCGTCGAAATTATTTACTACGGTCACGCCACGCGGCGGCGTAGGAGAACTGCAGGCAACGACCAGAAATGCAGCTGCCGCTGCGGCAACGAG                                                                                <  SRR4302537.3027997/94‑1 (MQ=60)

               TGGCGTCGAAATTATTTACTACGGTCACGCCACGCGGCGGCGTAGGAGAACTGCAGGCAACGACCAGAAATGCAGCTGCCGCTGCGGCAACGAG                                                                                <  SRR4302537.1513220/94‑1 (MQ=60)

               TGGCGTCGAAATTATTTACTACGGTCACGCCACGCGGCGGCGTAGGAGAACTGCAGGCAACGACCAGAAATGCAGCTGCCGCTGCGGCAACGAG                                                                                >  SRR4302537.3694758/1‑94 (MQ=60)

                GGCGTCAAAATTATTTACTACGGTCACGCCACGCGGCGGCGTAGGAGAACTGCAGGCAACGACCAGAAATGCAGCTGCCGCTGCGGCAACGAGA                                                                               <  SRR4302537.4730804/94‑1 (MQ=60)

                  GGTCGAAATTATTTACTACGGTCACGCCACGCGGCGGCGTAGGAGAACTGCAGGCAACGACCAGAAATGCAGCTGCCGCTGCGGCAACGAGAGG                                                                             <  SRR4302537.4625275/94‑1 (MQ=60)

                  CGTCGAAATTATTTACTACGGTCACGCCACGCGGCGGCGTAGGAGAACTGCAGGCAACGACCAGAAATGCAGCTGCCGCTGCGGCAACGAGAGG                                                                             >  SRR4302537.2167774/1‑94 (MQ=60)

                    TCGAAATTATTTACTACGGTCACGCCACGCGGCGGCGTAGGAGAACTGCAGGCAACGACCAGAAATGCAGCTGCCGCTGCGGCAACGAGAGGGA                                                                           >  SRR4302537.4540809/1‑94 (MQ=60)

                    TCGAAATTATTTACTACGGTCACGCCACGCGGCGGCGTAGGAGAACTGCAGGCAACGACCAGAAATGCAGCTGCCGCTGCGGCAACGAGAGGGA                                                                           <  SRR4302537.3925571/94‑1 (MQ=60)

                    TCGAAATTATTTACTACGGTCACGCCACGCGGCGGCGTAGGAGAACTGCAGGCAACGACCAGAAATGCAGCTGCCGCTGCGGCAACGAGAGGGA                                                                           <  SRR4302537.1735036/94‑1 (MQ=60)

                    GCGAAATTATTTACTACGGTCACGCCGCGCGGCGGCGTAGGAGAACTGCAGGCAACGACCAGAAATGCAGCTGCCGCTGCGGCAACGAGAGGGA                                                                           <  SRR4302537.2013192/94‑1 (MQ=60)

                     CGAAATTATTTACTACGGTCACGCCACGCGGCGGCGTAGGAGAACTGCAGGCAACGACCAGAAATGCAGCTGCCGCTGCGGCAACGAGAGGGAG                                                                          <  SRR4302537.5037158/94‑1 (MQ=60)

                     CGAAATTATTTACTACGGTCACGCCACGCGGCGGCGTAGGAGAACTGCAGGCAACGACCAGAAATGCAGCTGCCGCTGCGGCAACGAGAGGGAG                                                                          <  SRR4302537.4249136/94‑1 (MQ=60)

                     CGAAATTATTTACTACGGTCACGCCACGCGGCGGCGTAGGAGAACTGCAGGCAACGACCAGAAATGCAGCTGCCGCTGCGGCAACGAGAGGGAG                                                                          >  SRR4302537.4279909/1‑94 (MQ=60)

                      GAAATTATTTACTACGGTCACGCCACGCGGCGGCGTAGGAGAACTGCAGGCAACGACCAGAAATGCAGCTGCCGCTGCGGCAACGAGAGGGAGC                                                                         >  SRR4302537.3393262/1‑94 (MQ=60)

                      GAAATTATTTACTACGGTCACGCCACGCGGCGGCGTAGGAGAACTGCAGGCAACGACCAGAAATGCAGCTGCCGCTGCGGCAACGAGAGGGAGC                                                                         >  SRR4302537.5461836/1‑94 (MQ=60)

                       AAATTATTTACTACGGTCACGCCACGCGGCGGTGTAGGAGAACTGCAGGCAACGACCAGAAATGCAGCTGCCGCTGCGGCAACGAGAGGGAGCA                                                                        <  SRR4302537.5200787/94‑1 (MQ=60)

                       AAATTATTTACTACGGTCACGCCACGCGGCGGCGTAGGAGAACTGCAGGCAACGACCAGAAATGCAGCTGCCGCTGCGGCAACGAGAGGGAGCA                                                                        >  SRR4302537.3797192/1‑94 (MQ=60)

                        AATTATTTACTACGGTCACGCCACGCGGCGGCGTAGGAGAACTGCAGGCAACGACCAGAAATGCAGCTGCCGCTGCGGCAACGAGAGGGAGCAG                                                                       <  SRR4302537.5977543/94‑1 (MQ=60)

                           TATTTACTACGGTCACGCCACGCGGCGGCGTAGGAGAACTGCAGGCAACGACCAGAAATGCAGCTGCCGCTGCGGCAACGAGAGGGAGCAGGCG                                                                    >  SRR4302537.6281898/1‑94 (MQ=60)

                            ATTTACTACGGTCACGCCACGCGGCGGCGTAGGAGAACTGCAGGCAACGACCAGAAATGCAGCTGCCGCTGCGGCAACGAGAGGGAGCAGGCGC                                                                   >  SRR4302537.1029431/1‑94 (MQ=60)

                              TTACTACGGTCACGCCACGCGGCGGCGTAGGAGAACTGCAGGCAACGACCAGAAATGCAGCTGCCGCTGCGGCAACGAGAGGGAGCAGGCGCAT                                                                 <  SRR4302537.6155091/94‑1 (MQ=60)

                                ACTACGGTCACGCCACGCGGCGGCGTAGGAGAACTGCAGGCAACGACCAGAAATGCAGCTGCCGCTGCGGCAACGAGAGGGAGCAGGCGCATAA                                                               >  SRR4302537.5577791/1‑94 (MQ=60)

                                ACTACGGTCACGCCACGCGGCGGCGTAGGAGAACTGCAGGCAACGACCAGAAATGCAGCTGCCGCTGCGGCAACGAGAGGGAGCAGGCGCATAA                                                               <  SRR4302537.1190958/94‑1 (MQ=60)

                                ACTACGGTCACGCCACGCGGCGGCGTAGGAGAACTGCAGGCAACGACCAGAAATGCAGCTGCCGCTGCGGCAACGAGAGGGAGCAGGCGCATAA                                                               <  SRR4302537.2142832/94‑1 (MQ=60)

                                 CTACGGTCACGCCACGCGGCGGCGTATGAGAACTGCAGGCAACGACCAGAAATGCAGCTGCCGCTGCGGCAACGAGAGGGAGCAGGCGCATAAT                                                              >  SRR4302537.3458530/1‑94 (MQ=60)

                                 CTACGGTCACGCCACGCGGCGGCGTAGGAGAACTGCAGGCAACGACCAGAAATGCAGCTGCCGCTGCGGCAACGAGAGGGAGCAGGCGCATAAA                                                              >  SRR4302537.4740028/1‑94 (MQ=60)

                                  TACGGTCACGCCACGCGGCGGCGTAGGAGAACTGCAGGCAACGACCAGAAATGCAGCTGCCGCTGCGGCAACGAGAGGGAGCAGGCGCATAAAT                                                             <  SRR4302537.1150177/94‑1 (MQ=60)

                                   ACGGTCACGCCACGCGGCGGCGTAGGAGAACTGCAGGCAACGACCAGAAATGCAGCTGCCGCTGCGGCAACGAGAGGGAGCAGGCGCATAAATG                                                            <  SRR4302537.4793422/94‑1 (MQ=60)

                                   ACGGTCACGCCACGCGGCGGCGTAGGAGAACTGCAGGCAACGACCAGAAATGCAGCTGCCGCTGCGGCAACGAGAGGGAGCAGGCGCATAAATG                                                            <  SRR4302537.4192081/94‑1 (MQ=60)

                                   ACGGTCACGCCACGCGGCGGCGTAGGAGAACTGCAGGCAACGACCAGAAATGCAGCTGCCGCTGCGGCAACGAGAGGGAGCAGGCGCATAAATG                                                            >  SRR4302537.84719/1‑94 (MQ=60)

                                   ACGGTCACGCCACGCGGCGGCGTAGGAGAACTGCAGGCAACGACCAGAAATGCAGCTGCCGCTGCGGCAACGAGAGGGAGCAGGCGCATAAATG                                                            >  SRR4302537.5586487/1‑94 (MQ=60)

                                    CGGTCACGCCACGCGGCGGCGTAGGAGAACTGCAGGCAACGACCAGAAATGCAGCTGCCGCTGCGGCAACGAGAGGGAGCAGGCGCATAAATGT                                                           <  SRR4302537.5834661/94‑1 (MQ=60)

                                     GGTCACGCCACGCGGCGGCGTAGGAGAACTGCAGGCAACGACCAGAAATGCAGCTGCCGCTGCGGCAACGAGAGGGAGCAGGCGCATAAATGTT                                                          <  SRR4302537.5943441/94‑1 (MQ=60)

                                      GTCACGCCACGCGGCGGCGTAGGAGAACTGCAGGCAACGACCAGAAATGCAGCTGCCGCTGCGGCAACGAGAGGGAGCAGGCGCATAAATGTTT                                                         <  SRR4302537.5822532/94‑1 (MQ=60)

                                       TCACGCCACGCGGCGGCGTAGGAGAACTGCAGGCAACGACCAGAAATGCAGCTGCCGCTGCGGCAACGAGAGGGAGCAGGCGCATAAATGTTTC                                                        <  SRR4302537.6390037/94‑1 (MQ=60)

                                        CACGCCACGCGGCGGCGTAGGAGAACTGCAGGCAACGACCAGAAATGCAGCTGCCGCTGCGGCAACGAGAGGGAGCAGGCGCATAAATGTTTCC                                                       <  SRR4302537.3279918/94‑1 (MQ=60)

                                        CACGCCACGCGGCGGCGTAGGAGAACTGCAGGCAACGACCAGAAATGCAGCTGCCGCTGCGGCAACGAGAGGGAGCAGGCGCATAAATGTTTCC                                                       >  SRR4302537.155116/1‑94 (MQ=60)

                                        CACGCCACGCGGAGGCGTAGGAGAACTGCAGGCAACGACCAGAAATGCAGCTGCCGCTGCGGCAACGAGAGGGAGCAGGCGCATAAATGTTTCC                                                       <  SRR4302537.3760921/94‑1 (MQ=60)

                                          CGCCACGCGGCGGCGTAGGAGAACTGCAGGCAACGACCAGAAATGCAGCTGCCGCTGCGGCAACGAGAGGGAGCAGGCGCATAAATGTTTCCTT                                                     <  SRR4302537.1928552/94‑1 (MQ=60)

                                           GCCACGCGGCGGCGTAGGAGAACTGCAGGCAACGACCAGAAATGCAGCTGCCGCTGCGGCAACGAGAGGGAGCAGGCGCATAAATGTTTCCTTA                                                    <  SRR4302537.4530317/94‑1 (MQ=60)

                                           GCCACGCGGCGGCGTAGGAGAACTGCAGGCAACGACCAGAAATGCAGCTGCCGCTGCGGCAACGAGAGGGAGCAGGCGCATAAATGTTTCCTTA                                                    >  SRR4302537.5437022/1‑94 (MQ=60)

                                            CCACGCGGCGGCGTAGGAGAACTGCAGGCAACGACCAGAAATGCAGCTGCCGCTGCGGCAACGAGAGGGGGCAGGGGCATAAATGTTTCCTTAC                                                   >  SRR4302537.1750219/1‑94 (MQ=60)

                                            CCACGCGGCGGCGTAGGAGAACTGCAGGCAACGACCAGAAATGCAGCTGCCGCTGCGGCAACGAGAGGGAGCAGGCGCATAAATGTTTCCTTAC                                                   >  SRR4302537.1409544/1‑94 (MQ=60)

                                             canggnncngctTAGGAGANCTNNAGGCAACGACCAGAAATGCAGNTGCCGCTGCGGCAACGAGAGGGAGCAGGCGCATAAATGTTTCCTTACT                                                  <  SRR4302537.4796240/82‑1 (MQ=60)

                                             CACGCGGCGGCGTAGGAGAACTGCAGGCAACGACCAGAAATGCAGCTGCCGCTGCGGCAACGAGAGGGAGCAGGCGCATAAATGTTTCCTTACT                                                  >  SRR4302537.6413892/1‑94 (MQ=60)

                                             CACGCGGCGGCGTAGGAGAACTGCAGGCAACGACCAGAAATGCAGCTGCCGCTGCGGCAACGAGAGGGAGCAGGCGCATAAATGTTTCCTTACT                                                  <  SRR4302537.3442568/94‑1 (MQ=60)

                                             CACGCGGCGGCGTAGGAGAACTGCAGGCAACGACCAGAAATGCAGCTGCCGCTGCGGCAACGAGAGGGAGCAGGCGCATAAATGTTTCCTTACT                                                  <  SRR4302537.1986200/94‑1 (MQ=60)

                                              ACGCGGCGGCGTAGGAGAACTGCAGGCAACGACCAGAAATGCAGCTGCCGCTGCGGCAACGAGAGGGAGCAGGCGCATAAATGTTTCCTTACTG                                                 >  SRR4302537.1258100/1‑94 (MQ=60)

                                              ACGCGGCGGCGTAGGAGAACTGCAGGCAACGACCAGAAATGCAGCTGCCGCTGCGGCAACGAGAGGGAGCAGGCGCATAAATGTTTCCTTACTG                                                 <  SRR4302537.5629053/94‑1 (MQ=60)

                                                GCGGCGGCGTAGGAGAACTGCAGGCAACGACCAGAAATGCAGCTGCCGCTGCGGCAACGAGAGGGAGCAGGCGCATAAATGTTTCCTTACTGGT                                               <  SRR4302537.3649604/94‑1 (MQ=60)

                                                GCGGCGGCGTAGGAGAACTGCAGGCAACGACCAGAAATGCAGCTGCCGCTGCGGCAACGAGAGGGAGCAGGAGCATAAATGTTTCCTTACTGGT                                               >  SRR4302537.5311197/1‑94 (MQ=60)

                                                 GGGCGGCGTAGGAAAACTGCAGGCAACGACCAGAAATGCAGCTGCCGCTGCGGCAACGAGAGGGAGCAGGCGCATAAATGTTTCCTTACTGGTT                                              <  SRR4302537.2573942/94‑1 (MQ=60)

                                                 CGGCGGCGTAGGAGAACTGCAGGCAACGACCAGAAATGCAGCTGCCGCTGCGGCAACGAGAGGGAGCAGGCGCATAAATGTTTCCTTACTGGTT                                              <  SRR4302537.366893/94‑1 (MQ=60)

                                                 CGGCGGCGTAGGAGAACTGCAGGCAACGACCAGAAATGCAGCTGCCGCTGCGGCAACGAGAGGGAGCAGGCGCATAAATGTTTCCTTACTGGTT                                              <  SRR4302537.3381638/94‑1 (MQ=60)

                                                  GGCGGCGTAGGAGAACTGCAGGCAACGACCAGAAATGCAGCTGCCGCTGCGGCAACGAGAGGGAGCAGGCGCATAAATGTTTCCTTACTGGTTT                                             <  SRR4302537.198200/94‑1 (MQ=60)

                                                   GCGGCGTAGGAGAACTGCAGGCAACGACCAGAAATGCAGCTGCCGCTGCGGCAACGAGAGGGAGCAGGCGCATAAATGTTTCCTTACTGGTTTT                                            <  SRR4302537.333812/94‑1 (MQ=60)

                                                     GGCGTAGGAGAACTGCAGGCAACGACCAGAAATGCAGCTGCCGCTGCGGCAACGGGCGGGAGCAGGCGCATAAATGTTTCCTTACTGGTGTTTT                                          >  SRR4302537.5783433/1‑94 (MQ=60)

                                                     GGCGTAGGAGAACTGCAGGCAACGACCAGAAATGCAGCTGCCGCTGCGGCAACGAGAGGGAGCAGGCGCATAAATGTTTCCTTACTGGTTTTTT                                          >  SRR4302537.962570/1‑94 (MQ=60)

                                                     GGCGTAGGAGAACTGCAGGCAACGACCAGAAATGCAGCTGCCGCTGCGGCAACGAGAGGGAGCAGGCGCATAAATGTTTCCTTACTGGTTTTTT                                          >  SRR4302537.5238322/1‑94 (MQ=60)

                                                     GGCGTAGGAGAACTGCAGGCAACGACCAGAAATGCAGCTGCCGCTGCGGCAACGAGAGGGAGCAGGCGCATAAATGTTTCCTTACTGGTTTTTT                                          <  SRR4302537.606565/94‑1 (MQ=60)

                                                     GGCGTAGGAGAACTGCAGGCAACGACCAGAAATGCAGCTGCCGCTGCGGCAACGAGAGGGAGCAGGCGCATAAATGTTTCCTTACTGGTTTTTT                                          >  SRR4302537.3102922/1‑94 (MQ=60)

                                                       CGTAGGAGAACTGCAGGCAACGACCAGAAATGCAGCTGCCGCTGCGGCAACGAGAGGGAGCAGGCGCATAAATGTTTCCTTACTGGTTTTTTTC                                        >  SRR4302537.5668938/1‑94 (MQ=60)

                                                        GTAGGAGAACTGCAGGCAACGACCAGAAATGCAGCTGCCGCTGCGGCAACGAGAGGGAGCAGGCGCATAAATGTTTCCTTACTGGTTTTTTTCT                                       <  SRR4302537.1672169/94‑1 (MQ=60)

                                                        GTAGGAGAACTGCAGGCAACGACCAGAAATGCAGCTGCCGCTGCGGCAACGAGAGGGAGCAGGCGCATAAATGTTTCCTTACTGGTTTTTTTCT                                       >  SRR4302537.1658229/1‑94 (MQ=60)

                                                        GTAGGAGAACTGCAGGCAACGACCAGAAATGCAGCTGCCGCTGAGGCAACGAGAGGGAGCAGGCGCATAAATGTTTCCTTACTGGTTTTTTTCT                                       >  SRR4302537.3490803/1‑94 (MQ=60)

                                                         TAGGAGAACTGCAGGCAACGACCAGAAATGCAGCTGCCGCTGCGGCAACGAGAGGGAGCAGGCGCATAAATGTTTCCTTACTGGTTTTTTTCTA                                      <  SRR4302537.5200947/94‑1 (MQ=60)

                                                          AGGAGAACTGCAGGCAACGACCAGAAATGCAGCTGCCGCTGCGGCAACGAGAGGGAGCAGGCGCATAAATGTTTCCTTACTGGTTTTTTTCTAA                                     >  SRR4302537.1518734/1‑94 (MQ=60)

                                                           GGAGAACTGCAGGCAACGACCAGAAATGCAGCTGCCGCTGCGGCAACGAGAGGGAGCAGGCGCATAAATGTTTCCTTACTGGTTTTTTTCTAAG                                    <  SRR4302537.520218/94‑1 (MQ=60)

                                                             AGAACTGCAGGCAACGACCAGAAATGCAGCTGTCGCTGCGGCAACGAGAGGGAGCAGGCGCATAAATGTTTCCTTACTGGTTTTTTTCTAAGTG                                  >  SRR4302537.4058643/1‑94 (MQ=60)

                                                             AGAACTGCAGGCAACGACCAGAAATGCAGCTGTCGCTGCGGCAACGAGAGGGAGCAGGCGCATAAATGTTTCCTTACTGGTTTTTTTCTAAGTG                                  <  SRR4302537.1494926/94‑1 (MQ=60)

                                                             AGAACTGCAGGCAACGACCAGAAATGCAGCTGCCGCTGCGGCAACGAGAGGGAGCAGGCGCATAAATGTTTCCTTACTGGTTTTTTTCTAAGTG                                  <  SRR4302537.2631884/94‑1 (MQ=60)

                                                             AGAACTGCAGGCAACGACCAGAAATGCAGCTGCCGCTGCGGCAACGAGAGGGAGCAGGCGCATAAATGTTTCCTTACTGGTTTTTTTCTAAGTG                                  <  SRR4302537.4995590/94‑1 (MQ=60)

                                                             AGAACTGCAGGCAACGACCAGAAATCCAGCTGCCGCTGCGGCAACGAGAGGGAGCAGGCGCATAAATGTTTCCTTACTGGTTTTTTTCTAACTG                                  <  SRR4302537.6291750/94‑1 (MQ=60)

                                                               AACTGCAGGCAACGACCAGAAATGCAGCTGCCGCTGCGGCAACGAGAGGGAGCAGGCGCATAAATGTTTCCTTACTGGTTTTTTTCTAAGTGTA                                >  SRR4302537.4602658/1‑94 (MQ=60)

                                                               AACTGCAGGCAACGACCAGAAATGCAGCTGCCGCTGCGGCAACGAGAGGGAGCAGGCGCATAAATGTTTCCTTACTGGTTTTTTTCTAAGTGTA                                <  SRR4302537.3323792/94‑1 (MQ=60)

                                                               AACTGCAGGCAACGACCAGAAATGCAGCTGCCGCTGCGGCAACGAGAGGGAGCAGGCGCATAAATGTTTCCTTACTGGTTTTTTTCTAAGTGTA                                >  SRR4302537.5362308/1‑94 (MQ=60)

                                                               AACTGCAGGCAACGACCAGAAATGCAGCTGCCGCTGCGGCAACGAGAGGGAGCAGGCGCATAAATGTTTCCTTACTGGTTTTTTTCTAAGTGTA                                <  SRR4302537.4190679/94‑1 (MQ=60)

                                                               AACTGCAGGCAACGACCAGAAATGCAGCTGCCGCTGCGGCAACGAGAGGGAGCAGGCGCATAAATGTTTCCTTACTGGTTTTTTTCTAAGTGTA                                <  SRR4302537.5132304/94‑1 (MQ=60)

                                                                tctgcaggcaccgccatGAAATGCAGCTGCCGCTGCGGCAGCGAGAGGGAGCAGGCGCATAAATGTTTCGTTACTGGTTTTTTTCTAAGTGTAG                               <  SRR4302537.1232105/77‑1 (MQ=60)

                                                                ACTGCAGGCAACGACCAGAAATGCAGCTGCCGCTGCGGCAACGAGAGGGAGCAGGCGCATAAATGTTTCCTTACTGGTTTTTTTCTAAGTGTAG                               <  SRR4302537.1767802/94‑1 (MQ=60)

                                                                ACTGCAGGCAACGACCAGAAATGCAGCTGCCGCTGCGGCAACGAGAGGGAGCAGGCGCATAAATGTTTCCTTACTGGTTTTTTTCTAAGTGTAG                               >  SRR4302537.3579279/1‑94 (MQ=60)

                                                                ACTGCAGGCAACGACCAGAAATGCAGCTGCCGCTGCGGCAACGAGAGGGAGCAGGCGCATAAATGTTTCCTTACTGGTTTTTTTCTAAGTGTAG                               >  SRR4302537.5041502/1‑94 (MQ=60)

                                                                 CTGCAGGCAACGACCAGAAATGCAGCTGCCGCTGCGGCAACGAGAGGGAGCAGGCGCATAAATGTTTCCTTACTGGTTTTTTTCTAAGTGTAGA                              <  SRR4302537.1134013/94‑1 (MQ=60)

                                                                  TGCAGGCAGCGCCTAGAAATGCAGCTGCCGCTGCGGCAACGAGAGGGAGCAGGCGCATAAATGTTTCCTTACTGGTTTTTTTCTAAGTGTAGAT                             <  SRR4302537.3500374/94‑1 (MQ=60)

                                                                  TGCAGGCAACGACCAGAAATGCAGCTGCCGCTGCGGCAACGAGAGGGAGCAGGCGCATAAATGTTTCCTTACTGGTTTTTTTCTAAGTGTAGAT                             <  SRR4302537.2317489/94‑1 (MQ=60)

                                                                   GCAGGCAACGACCAGAAATGCAGCTGCCGCTGCGGCAACGAGAGGGAGCAGGCGCATAAATGTTTCCTTACTGGTTTTTTTCTAAGTGTAGATG                            <  SRR4302537.348905/94‑1 (MQ=60)

                                                                    CAGGCAACGACCAGAAATGCAGCTGCCGCTGCGGCAACGAGAGGGAGCAGGCGCATAAATGTTTCCTTACTGGTTTTTTTCTAAGTGTAGATGA                           >  SRR4302537.2242285/1‑94 (MQ=60)

                                                                     AGGCAACGACCAGAAATGCAGCTGCCGCTGCGGCAACGAGAGGGAGCAGGCGCATAAATGTTTCCTTACTGGTTTTTTTCTAAGTGTAGATGAC                          >  SRR4302537.1168309/1‑94 (MQ=60)

                                                                     AGGCAACGACCAGAAATGCAGCTGCCGCTGCGGCAACGAGAGGGAGCAGGCGCATAAATGTTTCCTTACTGGTTTTTTTCTAAGTGTAGATGAC                          <  SRR4302537.5849926/94‑1 (MQ=60)

                                                                      GGCAACGACCAGAAATGCAGCTGCCGCTGCGGCAACGAGAGGGAGCAGGCGCATAAATGTTTCCTTACTGGTTTTTTTCTAAGTGTAGATGACA                         <  SRR4302537.5132854/94‑1 (MQ=60)

                                                                      GGCAACGACCAGAAATGCAGCTGCCGCTGCGGCAACGAGAGGGAGCAGGCGCATAAATGTTTCCTTACTGGTTTTTTTCTAAGTGTAGATGACA                         <  SRR4302537.2704216/94‑1 (MQ=60)

                                                                      GGCAACGACCAGAAATGCAGCTGCCGCTGCGGCAACGAGAGGGAGCAGGCGCATAAATGTTTCCTTACTGGTTTTTTTCTAAGTGTAGATGACA                         <  SRR4302537.6055232/94‑1 (MQ=60)

                                                                      GGCAACGACCAGAAATGCAGCTGCCGCTACGGCAACGAGAGGGAGCAGGCGCATAAATGTTTCCTTACTGGTTTTTTTCTAAGTGTAGATGACA                         <  SRR4302537.6262467/94‑1 (MQ=60)

                                                                       GCAACGACCAGAAATGCAGCTGCCGCTGCGGCAACGAGAGGGAGCAGGCGCATAAATGTTTCCTTACTGGTTTTTTTCTAAGTGTAGATGACAG                        <  SRR4302537.5720759/94‑1 (MQ=60)

                                                                        CAACGACCAGAAATGCAGCTGCCGCTGCGGCAACGAGAGGGAGCAGGCGCATAAATGTTTCCTTACTGGTTTTTTTCTAAGTGTAGATGACAGC                       >  SRR4302537.3052507/1‑94 (MQ=60)

                                                                        CAACGACCAGAAATGCAGCTGCCGCTGCGGCAACGAGAGGGAGCAGGCGCATAAATGTTTCCTTACTGGTTTTTTTCTAAGTGTAGATGACAGC                       >  SRR4302537.3408487/1‑94 (MQ=60)

                                                                         AACGACCAGAAATGCAGCTGCCGCTGCGGCAACGAGAGGGAGCAGGCGCATAAATGTTTCCTTACTGGTTTTTTTCTAAGTGTAGATGACAGCA                      <  SRR4302537.5919075/94‑1 (MQ=60)

                                                                           CGACCAGAAATGCAGCTGCCGCTGCGGCAACGAGAGGGAGCAGGCGCATAAATGTTTCCTTACTGGTTTTTTTCTAAGTGTAGATGACAGCAAG                    <  SRR4302537.4405615/94‑1 (MQ=60)

                                                                           CGACCAGAAATGCAGCTGCCGCTGCGGCAACGAGAGGGAGCAGGCGCATAAATGTTTCCTTACTGGTTTTTTTCTAAGTGTAGATGACAGCAAG                    <  SRR4302537.747620/94‑1 (MQ=60)

                                                                            GACCAGAAATGCAGCTGCCGCTGCGGCAACGAGAGGGAGCAGGCGCATAAATGTTTCCTTACTGGTTTTTTTCTAAGTGTAGATGACAGCAAGG                   >  SRR4302537.1021119/1‑94 (MQ=60)

                                                                            GACCAGAAATGCAGCTGCCGCTGCGGCAACGAGAGGGAGCAGGCGCATAAATGTTTCCTTACTGGTTTTTTTCTAAGTGTAGATGACAGCAAGG                   <  SRR4302537.880709/94‑1 (MQ=60)

                                                                             ACCAGAAATGCAGCTGCCGCTGCGGCAACGAGAGGGAGCAGGCGCATAAATGTTTCCTTACTGGTTTTTTTCTAAGTGTAGATGACAGCAAGGA                  <  SRR4302537.921577/94‑1 (MQ=60)

                                                                             ACCAGAAATGCAGCTGCCGCTGCGGCAACGAGAGGGAGCAGGCGCATAAATGTTTCCTTACTGGTTTTTTTCTAAGTGTAGATGACAGCAAGGA                  >  SRR4302537.5633136/1‑94 (MQ=60)

                                                                              CCAGAAATGCAGCTGCCGCTGCGGCAACGAGAGGGAGCAGGCGCATAAATGTTTCCTTACTGGTTTTTTTCTAAGTGTAGATGACAGCAAGGAA                 <  SRR4302537.529252/94‑1 (MQ=60)

                                                                              CCAGAAAGGCAGCTGCCGCTGCGGCAACGAGAGGGAGCAGGCGCATAAATGTTTCCTTACTGGTTTTTTTCTAAGTGTAGATGACAGCAAGGAA                 <  SRR4302537.4842804/94‑1 (MQ=60)

                                                                                AGAAATGCAGCTGCCGCTGCGGCAACGAGAGGGAGCAGGCGCATAAATGTTTCCTTACTGGTTTTTTTCTAAGTGTAGATGACAGCAAGGAAAA               >  SRR4302537.3061238/1‑94 (MQ=60)

                                                                                 GAAATGCAGCTGCCGCTGCGGCAACGAGAGGGAGCAGGCGCATAAATGTTTCCTTACTGGTTTTTTTCTAAGTGTAGATGACAGCAAGGAAAAG              >  SRR4302537.4666520/1‑94 (MQ=60)

                                                                                 GAAATGCAGCTGCCGCTGCGGCAACGAGAGGGAGCAGGCGCATAAATGTTTCCTTACTGGTTTTTTTCTAAGTGTAGATGACAGCAAGGAAAAG              <  SRR4302537.960661/94‑1 (MQ=60)

                                                                                 GAAATGCAGCTGCCGCTGCGGCAACGAGAGGGAGCAGGCGCATAAATGTTTCCTTACTGGTTTTTTTCTAAGTGTAGATGACAGCAAGGAAAAG              <  SRR4302537.3650257/94‑1 (MQ=60)

                                                                                  AAATGCAGCTGCCGCTGCGGCAACGAGAGGGAGCAGGCGCATAAATGTTTCCTTACTGGTTTTTTTCTAAGTGTAGATGACAGCAAGGAAAAGC             <  SRR4302537.5790098/94‑1 (MQ=60)

                                                                                      GCAGCTNCCGCTGCGGCAACGAGAGGGAGCAGGCGCATAAATGTTTCCTTACTGGTTTTTTTCTAAGTGTAGATGACAGCAAGGAAAAGCGGAG         <  SRR4302537.1460072/94‑1 (MQ=60)

                                                                                      GCAGCTGCCGCTGCGGCAACGAGAGGGAGCAGGCGCATAAATGTTTCCTTACTGGTTTTTTTCTAAGTGTAGATGACAGCAAGGAAAAGCGGAG         <  SRR4302537.5526075/94‑1 (MQ=60)

                                                                                      GCAGCTGCCGCTGCGGCAACGAGAGGGAGCAGGCGCATAAATGTTTCCTTACTGGTTTTTTTCTAAGTGTAGATGACAGCAAGGAAAAGCGGAG         >  SRR4302537.2032393/1‑94 (MQ=60)

                                                                                       CAGCTGTCGCTGCGGCAACGAGAGGGAGCAGGCGCATAAATGTTTCCTTACTGGTTTTTTTCTAAGTGTAGATGACAGCAAGGAAAAGCGGAGA        >  SRR4302537.1287928/1‑94 (MQ=60)

                                                                                       CAGCTGCCGCTGCGGCAACGAGAGGGAGCAGGCGCATAAATGTTTCCTTACTGGTTTTTTTCTAAGTGTAGATGACAGCAAGGAAAAGCGGAGA        >  SRR4302537.2234451/1‑94 (MQ=60)

                                                                                       CAGCTGCCGCTGCGGCAACGAGAGGGAGCAGGCGCATAAATGTTTCCTTACTGGTTTTTTTCTAAGTGTAGATGACAGCAAGGAAAAGCGGAGA        <  SRR4302537.5261761/94‑1 (MQ=60)

                                                                                       CAGCTGCCGCTGCGGCAACGAGAGGGAGCAGGCGCATAAATGTTTCCTTACTGGTTTTTTTCTAAGTGTAGATGACAGCAAGGAAAAGCGGAGA        <  SRR4302537.4428313/94‑1 (MQ=60)

                                                                                         GCTGCCGCTGCGGCAACGAGAGGGAGCAGGCGCATAAATGTTTCCTTACTGGTTTTTTTCTAAGTGTAGATGACAGCAAGGAAAAGCGGAGAAA      <  SRR4302537.3760221/94‑1 (MQ=60)

                                                                                         GCTGCCGCTGCGGCAACGAGAGGGAGCAGGCGCATAAATGTTTCCTTACTGGTTTTTTTCTAAGTGTAGATGACAGCAAGGAAAAGCGGAGAAA      <  SRR4302537.2705924/94‑1 (MQ=60)

                                                                                             CCGCTGCGGCAACGAGAGGGAGCAGGCGCATAAATGTTTCCTTACTGGTTTTTTTCTAAGTGTAGATGACAGCAAGGAAAAGCGGAGAAAAGGT  <  SRR4302537.4837941/94‑1 (MQ=60)

                                                                                             CCGCTGCGGCAACGAGAGGGAGCAGGCGCATAAATGTTTCCTTACTGGTTTTTTTCTAAGTGTAGATGACAGCAAGGAAAAGCGGAGAAAAGGT  >  SRR4302537.5674917/1‑94 (MQ=60)

                                                                                             CCGCTGCGGCAACGAGAGGGAGCAGGCGCATAAATGTTTCCTTACTGGTTTTTTTCTAAGTGTAGATGACAGCAAGGAAAAGCGGAGAAAAGGT  <  SRR4302537.1837215/94‑1 (MQ=60)

TACCGAGATAACGTTTGGCGTCGAAATTATTTACTACGGTCACGCCACGCGGCGGCGTAGGAGAACTGCAGGCAACGACCAGAAATGCAGCTGTCGCTGCGGCAACGAGAGGGAGCAGGCGCATAAATGTTTCCTTACTGGTTTTTTTCTAAGTGTAGATGACAGCAAGGAAAAGCGGAGAAAAGGT  >  NC_000913/4375177‑4375363

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 9 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`