Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 882 254.4 12957588 98.0% 12698436 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,384,262 T→C E896G (GAG→GGG)  yjeP ← putative periplasmic binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,384,2620TC100.0% 809.4 / NA 231E896G (GAG→GGG) yjePputative periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (119/112);  total (119/112)

Only 100 of 238 total aligned reads displayed.

AGACCAGAGATAAAGTTGGCGAAAATTTCCTGCAAACCAAAACCGAGACCAACACCGAGCGCGGCAACCAGCCACTGCAATTTCGACCACTCAATACCAATCATTGAGAAGCCGACCAGCCCGCCAATCAGCATCAGCAGATATTTGGTGATGGTGGTGATGGCGTAACCCGTACCCGGC  >  NC_000913/4384172‑4384351
                                                                                          |                                                                                         
aGACCAGAGATAAAGTTGGCGAAAATTTCCTGCAAACCAAAACCGAGACCAACACCGAGCGCGGCAACCAGCCACTGCAATTTCGACCACCCa                                                                                         >  2:4560516/1‑93 (MQ=255)
 gACCAGAGATAAAGTTGGCGAAAATTTCCTGCAAACCAAAACCGAGACCAACCCCGAGCGCGGCAACCAGCCACTGCAATTTCGACCACCCaa                                                                                        <  2:207789/93‑1 (MQ=255)
  acgagagATAAGGTTGGCGAAAATTTCCTGCAAACCAAAACCGAGACCAACACCGAGCGCGGCAACCAGCCACTGCAATTTCGGCCACCCAAt                                                                                       <  2:1876823/90‑1 (MQ=255)
     agagATAAAGTTGGCGAAAATTTCCTGCAAACCAAAACCGAGACCAACACCGAGCGCGGCAACCAGCCACTGCAATTTCGACCACCCAATAcc                                                                                    <  1:5915524/93‑1 (MQ=255)
      gagaTAAAGTTGGCGAAAATTTCCTGCAAACCAAAACCGAGACCAACACCGAGCGCGGCAACCAGCCACTGCAATTTCGACCACCCAATACCa                                                                                   <  2:1409525/93‑1 (MQ=255)
      gagaTAAAGTTGGCGAAAATTTCCTGCAAACCAAAACCGAGACCAACACCGAGCGCGGCAACCAGCCACTGCAATTTAGACCACCCAATACCa                                                                                   >  2:3002780/1‑93 (MQ=255)
       agaTAAAGTTGGCGAAAATTTCCTGCAAACCAAAACCGAGACCAACACCGAGCGCGGCAACCAGCCACTGCAATTTCGACCACCCAATACCaa                                                                                  <  1:3890269/93‑1 (MQ=255)
       agaTAAAGTTGGCGAAAATTTCCTGCAAACCAAAACCGAGACCAACACCGAGCGCGGCAACCAGCCACTGCAATTTCGACCAACCAATACCaa                                                                                  >  2:2817852/1‑93 (MQ=255)
        gaTAAAGTTGGCGAAAATTTCCTGCAAACCAAAACCGAGACCAACACCGAGCGCGGCAACCAGCCACTGCAATTTCGACCACCCAATACCAAt                                                                                 >  2:726660/1‑93 (MQ=255)
        gaTAAAGTTGGCGAAAATTTCCTGCAAACCAAAACAGAGACCAACACCGAGCGCGGCAACCAGCCACTGCCATTTCGACCACCCAATACCAAt                                                                                 >  2:3847010/1‑93 (MQ=255)
         aTAAAGTTGGCGAAAATTTCCTGCAAACCAAAACCGAGACCAACACCGAGCGCGGCAACCAGCCACTGCAATTTCGACCACCCAATACCAATc                                                                                <  1:6131356/93‑1 (MQ=255)
         aTAAAGTTGGCGAAAATTTCCTGCAAACCAAAACCGAGACCAACACCGAGCGCGGCAACCAGCCACTGCAATTTCGACCACCCAATACCAATc                                                                                <  2:3136294/93‑1 (MQ=255)
         aTAAAGTTGGCGAAAATTTCCTGCAAACCAAAACCGAGACCAACACCGAGCGCGGCAACCAGCCACTGCAATTTCGACCACCCAATACCAATc                                                                                >  1:18966/1‑93 (MQ=255)
          tAAAGTTGGCGAAAATTTCCTGCAAACCAAAACCGAGACCAACACCGAGCGCGGCAACCAGCCACTGCAATTTCGACCACCCAATACCAATCa                                                                               >  2:5673369/1‑93 (MQ=255)
           aaaGTTGTCGAAAATTTCCTGCAAACCAAAACCGAGACCAACACCGAGCGCGGCAACCAGCCACTGCAATTTCGACCACCCAATACCAATTAt                                                                              <  2:2063664/93‑1 (MQ=255)
            aaGTTGGCGAAAATTTCCTGCAAACCAAAACCGAGACCAACACCGAGCGCGGCAACCAGCCACTGCAATTTCGACCACCCAATACCAATCAtt                                                                             >  2:5796339/1‑93 (MQ=255)
              gTTGGCGAAAATTTCCTGCAAACCAAAACCGAGACCAACACCGAGCGCGGCAACCAGCCACTGCAATTTCGACCACCCAATACCAATCATTga                                                                           >  1:1693127/1‑93 (MQ=255)
              gTTGGCGAAAATTTCCTGCAAACCAAAACCGAGACCAACACCGAGCGCGGCAACCAGCCACTGCAATTTCGACCACCCAATACCAATCATTga                                                                           <  1:4800868/93‑1 (MQ=255)
               ttGGCGAAAATTTCCTGCAAACCAAAACCGAGACCAACACCGAGCGCGGCAACCAGCCACTGCAATTTCGACCACCCAATACCAATCATTgag                                                                          >  2:2222062/1‑93 (MQ=255)
               ttGGCGAAAATTTCCTGCAAACCAAAACCGAGACCAACACCGAGCGCGGCAACCAGCCACTGCAATTTCGACCACCCAATACCAATCATTgag                                                                          <  2:4779518/93‑1 (MQ=255)
               ttGGCGAAAATTTCCTGCAAACCAAAACCGAGACCAACACCGAGCGCGGCAACCAGCCACTGCAATTTCGACCACCCAATACCAATCATTgag                                                                          >  1:1476305/1‑93 (MQ=255)
                  gCGAAAATTTCCTGCAAACCAAAACCGAGACCAACACCGAGCGCGGCAACCAGCCACTGCAATTTCGACCACCCAATACCAATCATTGAGAAg                                                                       <  2:5567053/93‑1 (MQ=255)
                  gCGAAAATTTCCTGCAAACCAAAACCGAGACCAACACCGAGCGCGGCAACCAGCCACTGCAATTTCGACCACCCAATACCAATCATTGAGAAg                                                                       <  1:4311003/93‑1 (MQ=255)
                   cGAAAATTTCCTGCAAACCAAACCCGAGACCAACACCGAGCGCGGCAACCAGCCACTGCAATTTCGACCACCCAATACCAATCATTGAGAAGc                                                                      <  2:370313/93‑1 (MQ=255)
                   cGAAAATTTCCTGCAAACCAAAACCGAGACCAACACCGAGCGCGGCAACCAGCCACTGCAATTTCGACCACCCAATACCAATCATTGAGAAGc                                                                      <  2:5218585/93‑1 (MQ=255)
                    gAAAATTTCCTGCAAACCAAAACCGAGACCAACACCGAGCGCGGCAACCAGCCACTGCAATTTCGACCACCCAATACCAATCATTGAGAAGcc                                                                     >  2:4017506/1‑93 (MQ=255)
                    gAAAATTTCCTGCAAACCAAAACCGAGACCAACACCGAGCGCGGCAACCAGCCACTGCAATTTCGACCACCCAATACCAATCATTGAGAAGcc                                                                     >  1:2049205/1‑93 (MQ=255)
                    gAAAATTTCCTGCAAACCAAAACCGAGACCAACACCGAGCGCGGCAACCAGCCACTGCAATTTCGACCACCCAATACCAATCATTGAGAAGcc                                                                     <  1:437799/93‑1 (MQ=255)
                    gAAAATTTCCTGCAAACCAAAACCGAGACCAACACCGAGCGCGGCAACCAGACACTGCAATTTCGACCACCCAATACCAATCATTGAGAAccc                                                                     >  2:2632562/1‑93 (MQ=255)
                        aTTTCCTGCAAACCAAAACCGAGACCAACACCGAGCGCGGCAACCAGCCACTGCAATTTCGACCACCCAATGCCAATCATTGAGAAGCCGAcc                                                                 >  2:2355661/1‑93 (MQ=255)
                         tttCCTGCAAACCAAAACCGAGACCAACACCGAGCGCGGCAACCAGCCACTGCAATTTCGACCACCCAATACCAATCATTGAGAAGCCGACCa                                                                >  1:5507848/1‑93 (MQ=255)
                          ttCCTGCAAACCAAAACCGAGACCAACACCGAGCGCGGCAACCAGCCACTGCAATTTCGACCACCCAATACCAATCATTGAGAAGCCGACCAg                                                               <  1:3511599/93‑1 (MQ=255)
                              tGCAAACCAAAACCGAGACCAACACCGAGCGCGGCAACCAGCCACTGCAATTTCGACCACCCAATACCAATCATTGAGAAGCCGACCAGCCCg                                                           >  1:3419195/1‑93 (MQ=255)
                              tGCAAACCAAAACCGAGACCAACACCGAGCGCGGCAACCAGCCACTGCAATTTCGACCACCCAATACCAATCATTGAGAAGCCGACCAGCCCg                                                           >  2:2291703/1‑93 (MQ=255)
                               gCAAACCAAAACCGAGACCAACACCGAGCGCGGCAACCAGCCACTGCAATTTCGACCACCCAATACCAATCATTGAGAAGCCGACCAGCCCGc                                                          <  2:4153627/93‑1 (MQ=255)
                                  aaCCAAAACCGAGACCAACACCGAGCGCGGCAACCAGCCACTGCAATTTCGACCACCCAATACCAATCATTGAGAAGCCGACCAGCCCGCCaa                                                       <  1:699837/93‑1 (MQ=255)
                                   aCCAAAACCGAGACCAACACCGAGCGCGGCAACCAGCCACTGCAATTTCGACCACCCAATACCAATCATTGAGAAGCCGACCAGCCCGCCAat                                                      >  2:327415/1‑93 (MQ=255)
                                   aCCAAAACCGACACCACCACCATGCGCGCCACCCAGCCACTGCAATTTCGACCACCCAATACCAATCATTGAGAAGCCGACCAGCCCGCCAat                                                      <  1:6338151/93‑1 (MQ=255)
                                    ccAAAACCGAGACCAACACCGAGCGCGGCAACCAGCCACTGCAATTTCGACCACCCAATACCAATCATTGAGAAGCCGACCAGCCCGCCAatc                                                     >  1:4966656/1‑93 (MQ=255)
                                    ccAAAACCGAGACCAACACCGAGCGCGGCAACCAGCCACTGCAATTTCGACCACCCAATACCAATCATTGAGAAGCCGACCAGCCCGCCAatc                                                     <  1:40296/93‑1 (MQ=255)
                                    ccAAAACCGAGACCAACACCGAGCGCGGCAACCAGCCACTGCAATTTCGACCACCCAATACCAATCATTGAGAAGCCGACCAGCCCGCCAatc                                                     <  2:620232/93‑1 (MQ=255)
                                      aaaaCCGAGACCAACACCGAGCGCGGCAACCAGCCACTGCAATTTCGACCACCCAATACCAATCATTGAGAAGCCGACCAGCCCGCCAatcag                                                   <  1:842606/93‑1 (MQ=255)
                                      aaaaCCGAGACCAACACCGAGCGCGGCAACCAGCCACTGCAATTTCGACCACCCAATACCAATCATTGAGAAGCCGACCAGCCCGCCAatcag                                                   <  1:1056504/93‑1 (MQ=255)
                                       aaaCCGAGACCACCCCCGAGCGCGGCACCCAGCCACTGCAATTTCGACCACCCAATACCAATCATTGAGAAGCCGACCAGCCCGCCAatcagc                                                  <  1:3722770/93‑1 (MQ=255)
                                        aaCCGAGACCAACACCGAGCGCGGCAACCAGCCACTGCAATTTCGACCACCCAATACCAATCATTGAGAAGCCGACCAGCCCGCCAatcagca                                                 <  1:6145161/93‑1 (MQ=255)
                                        aaCCGAGACCAACACCGAGCGCGGCAACCAGCCACTGCAATTTCGACCACCCAATACCAATCATTGAGAAGCCGACCAGCCCGCCAatcagca                                                 >  1:3063253/1‑93 (MQ=255)
                                        aaCCGAGACCAACACCGAGCGCGGCAACCAGCCACTGCAATTTCGACCACCCAATACCAATCATTGAGAAGCCGACCAGCCCGCCAatcagca                                                 <  2:3560862/93‑1 (MQ=255)
                                         aCCGAGACCAACACCGAGCGCGGCAACCAGCCACTGCAATTTCGACCACCCAATACCAATCATTGAGAAGCCGACCAGCCCGCCAatcagcat                                                >  1:5204036/1‑93 (MQ=255)
                                         aCCGAGACCAACACCGAGCGCGGCAACCAGCCACTGCAATTTCGACCACCCAATACCAATCATTGAGAAGCCGACCAGCCCGCCAatcagcat                                                >  2:2462858/1‑93 (MQ=255)
                                         aCCGAGACCAACACCGAGCGCGGCAACCAGCCACTGCAATTTCGACCACCCAATACCAATCATTGAGAAGCCGACCAGCCCGCCAatcagcat                                                >  1:5776399/1‑93 (MQ=255)
                                         aCCGAGACCAACACCGAGCGCGGCAACCAGCCACTGCAATTTCGACCACCCAATACCAATCATTGAGAAGCCGACCAGCCCGCCAatcagcat                                                >  1:5860486/1‑93 (MQ=255)
                                           cGAGACCAACACCGAGCGCGGCAACCAGCCACTGCAATTTCGACCACCCAATACCAATCATTGAGAAGCCGACCAGCCCGCCAatcaacatca                                              >  1:2209188/1‑93 (MQ=255)
                                            gagaCCAACACCGAGCGCGGCAACCAGCCACTGCAATTTCGACCACCCAATACCAATCATTGAGAAGCCGACCAGCCCGCCAatcagcatcag                                             >  2:5916050/1‑93 (MQ=255)
                                             agaCCAACACCGAGCGCGGCAACCAGCCACTGCAATTTCGACCACCCAATACCAATCAATGAGAAGACGAACAGCCCGCCAatcagcatcagc                                            >  1:240131/1‑93 (MQ=255)
                                              gaCCAACACGGAGCGCGGCAACCAGCCACTGCAATTTCGACCACCCAATACCAATCATTGAGAAGCCGACCAGCCCGCCAAtcagcatcagca                                           <  1:2889087/93‑1 (MQ=255)
                                              gaCCAACACCGAGCGCGGCCACCAGCCACTGCAATTTCGACCACCCAATACCAATCATTGAGAAGCCGACCAGCCCCCCAAtcagcatcagca                                           >  2:5738383/1‑93 (MQ=255)
                                              gaCCAACACCGAGCGCGGCAACCAGCCACTGCAATTTCGACCACCCAATACCAATCATTGAGAAGCCGACCAGCCCGCCAAtcagcatcagca                                           >  2:2506592/1‑93 (MQ=255)
                                                ccAACCCCGAGCGCGGCAACCAGCCACTGCAATTTCGACCACCCAATACCAATCATTGAGAAGCCGACCAGCCCGCCAATCAGCATCAGCAGa                                         <  2:4478860/93‑1 (MQ=255)
                                                 cAACACCGAGCGCGGCAACCAGCCACTGCAATTTCGACCACCCAATACCAATCATTGAGAAGCCGACCAGCCCGCCAATCAGCATCAGCAGat                                        >  1:5024510/1‑93 (MQ=255)
                                                  aaCACCGAGCGCGGCAACCAGCCACTGCAATTTCGACCACCCAATACCAATCATTGAGAAGCCGACCAGCCCGCCAATCAGCATCAGCAGata                                       <  1:3293460/93‑1 (MQ=255)
                                                  aaCACCGAGCGCGGCAACCAGCCACTGCAATTTCGACCACCCAATACCAATCATTGAGAAGCCGACCAGCCCGCCAATCAGCATCAGCAGata                                       >  1:4568219/1‑93 (MQ=255)
                                                  aaCACCGAGCGCGGCAACCAGCCACTGCAATTTCGACCACCCAATACCAATCATTGAGAAGCCGACCAGCCCGCCAATCAGCATCAGCAGata                                       >  1:932799/1‑93 (MQ=255)
                                                    cacCGAGCGCGGCAACCAGCCACTGCAATTTCGACCACCCAATACCAATCATTGAGAAGCCGACCAGCCCGCCAATCAGCATCAGCAGATAtt                                     >  2:2041144/1‑93 (MQ=255)
                                                     cccGAGCGCGGCAACCAGCCACTGCAATTTCGACCACCCAATACCAATCATTGAGAAGCCGACCAGCCCGCCAATCAGCATCAGCAGATAttt                                    <  1:5409715/92‑1 (MQ=255)
                                                     acCGAGCGCGGCAACCAGCCACTGCAATTTCGACCACCCAATACCAATCATTGAGAAGCCGACCAGCCCGCCAATCAGCATCAGCAGATAttt                                    <  1:2881246/93‑1 (MQ=255)
                                                      ccGAGCGCGGCAACCAGCCACTGCAATTTCGACCACCCAATACCAATCATTGAGAAGCCGACCAGCCCGCCAATCAGCATCAGCAGATATTtg                                   <  2:490098/93‑1 (MQ=255)
                                                      ccGAGCGCGGCAACCAGCCACTGCAATTTCGACCACCCAATACCAATCATTGAGAAGCCGACCAGCCCGCCAATCAGCATCAGCAGATATTtg                                   >  2:762717/1‑93 (MQ=255)
                                                       cGAGCGCGGCAACCAGCCACTGCAATTTCGACCACCCAATACCAATCATTGAGAAGCCGACCAGCCCGCCAATCAGCATCAGCAGATATTtgg                                  <  1:6031568/93‑1 (MQ=255)
                                                        gAGCGCGGCAACCAGCCACTGCAATTTCGACCACCCAATACCAATCATTGAGAAGCCGACCAGCCCGCCAATCAGCATCAGCAGATATTtggt                                 >  1:103108/1‑93 (MQ=255)
                                                         ggcgcGGCACCCAGCCACTGCAATTTCGACCACCCAATACCAATCATTGAGAAGCCGACCAGCCCGCCAATCAGCATCAGCAGATATTtggtg                                <  2:6048/92‑1 (MQ=255)
                                                           cgcgGCAACCAGCCACTGCAATTTCGACCACCCAATACCAATCATTGAGAAGCCGACCAGCCCGCCAATCAGCATCAGCAGATATTtggtgat                              <  2:1141542/93‑1 (MQ=255)
                                                           cgcgGCAACCAGCCACTGCAATTTCGACCACCCAATACCAATCATTGAGAAGCCGACCAGCCCGCCAATCAGCATCAGCAGATATTtggtgat                              <  1:6468081/93‑1 (MQ=255)
                                                            gcgGCAACCAGCCACTGCAATTTCGACCACCCAATACCAATCATTGAGAAGCCGACCAGCCCGCCAATCAGCATCAGCAGATATTtggtgatg                             >  1:3760791/1‑93 (MQ=255)
                                                             cgGCAACCAGCCACTGCAATTTCGACCACCCAATACCAATCATTGAGAAGCCGACCAGCCCGCCAATCAGCATCAGCAGATATTtggtgatgg                            <  2:1359031/93‑1 (MQ=255)
                                                              ggCAACCAGCCACTGCAATTTCGACCACCCAATACCAATCATTGAGAAGCCGACCAGCCCGCCAATCAGCATCAGCAGATATTtggtgatggt                           <  1:6250026/93‑1 (MQ=255)
                                                              cgCAACCAGCCACTTCAATTTCGACCCCCCAATACCAATCATTGAGAAGCCGACCAGCCCGCCAATCAGCATCAGCAGATATTtggtgatggt                           <  2:1062598/92‑1 (MQ=255)
                                                                cAACCAGCCACTGCAATTTCGACCACCCAATACCAATCATTGAGAAGCCGACCAGCCCGCCAATCAGCATCAGCAGATATTtggtgatggtgg                         >  1:4156541/1‑93 (MQ=255)
                                                                 acccAGCCACTGCAATTTCGACCACCCAATACCAATCATTGAGAAGCCGACCAGCCCGCCAATCAGCATCAGCAGATATTtggtgatggtggt                        <  1:2620234/91‑1 (MQ=255)
                                                                 aaCCAGCCACTGCAATTTCGACCACCCAATACCAATCATTGAGAAGCCGACCAGCCCGCCAATCAGCATCAGCAGATATTtggtgatggtggt                        <  1:5723226/93‑1 (MQ=255)
                                                                  aCCAGCCACTGCAATTTCGACCACCCAATACCAATCATTGAGAAGCCGACCAGCCCGCCAATCAGCATCAGCAGATATTtggtgatggtggtg                       >  2:5092436/1‑93 (MQ=255)
                                                                   ccAGCCACTGCAATTTCGACCACCCAATACCAATCATTGAGAAGCCGACCAGCCCGCCAATCAGCATCAGCAGATATTtggtgatggtggtga                      >  1:661249/1‑93 (MQ=255)
                                                                    cAGCCACTGCAATTTCGACCACCCAATACCAATCATTGAGAAGCCGACCAGCCCGCCAATCAGCATCAGCAGATATTtggtgatggtggtgat                     >  1:6176978/1‑93 (MQ=255)
                                                                    cAGCCACTGCAATTTCGACCACCCAATACCAATCATTGAGAAGCCGACCAGCCCGCCAATCAGCATCAGCAGATATTtggtgatggtggtgat                     <  1:4740877/93‑1 (MQ=255)
                                                                     aGCCACTGCAATTTCGACCACCCAATACCAATCATTGAGAAGCCGACCAGCCCGCCAATCAGCATCAGCAGATATTtggtgatggtggtgatg                    >  1:163832/1‑93 (MQ=255)
                                                                     aGCCACTGCAATTTCGACCACCCAATACCAATCATTGAGAAGCCGACCAGCCCGCCAATCAGCATCAGCAGATATTtggtgatggtggtgatg                    <  2:2711578/93‑1 (MQ=255)
                                                                     aGCCACTGCAATTTCGACCACCCAATACCAATCATTGAGAAGCCGACCAGCCCGCCAATCAGCATCAGCAGATATTtggtgatggtggtgatg                    <  2:5472415/93‑1 (MQ=255)
                                                                     aGCCACTGCAATTTCGACCACCCAATACCAATCATTGAGAAGCCGACCAGCCCGCCAATCAGCATCAGCAGATATTtggtgatggtggtgatg                    <  2:5775782/93‑1 (MQ=255)
                                                                     aGCCACGGCAATTTCGACCACCCAATACCAATCATTGAGAAGCCGACCAGCCCGCCAATCAGCATCAGCAGATATTtggtgatggtggtgatg                    <  2:4435594/93‑1 (MQ=255)
                                                                      gCCACTGCAATTTCGACCACCCAATACCAATCATTGAGAAGCCGACCAGCCCGCCAATCAGCATCAGCAGATATTtggtgatggtggtgatgg                   >  1:51137/1‑93 (MQ=255)
                                                                      gCCACTGCAATTTCGACCACCCAATACCAATCATTGAGAAGCCGACCAGCCCGCCAATCAGCATCAGCAGATATTtggtgatggtggtgatgg                   <  2:3061035/93‑1 (MQ=255)
                                                                      gCCACTGCAATTTCGACCACCCAATACCAATCATTGAGAAGCCGACCAGCCCGCCAATCAGCATCAGCAGATATTtggtgatggtggtgatgg                   >  2:1845948/1‑93 (MQ=255)
                                                                          cTGCAATTTCGCCCCCCCAATCCCAATCATTGAGAAGCCGACCAGCCCGCCAATCAGCATCAGCAGATATTTGGTGATGGTGGTGATGGCGTa               <  2:4425750/93‑1 (MQ=255)
                                                                           tGCAATTTCGACCACCCAATACCAATCATTGAGAAGCCGACCAGCCCGCCAATCAGCATCAGCAGATATTTGGTGATGGTGGTGATGGCGTaa              >  2:4609612/1‑93 (MQ=255)
                                                                            gCAATTTCGACCACCCAATACCAATCATTGAGAAGCCGACCAGCCCGCCAATCAGCATCAGCAGATATTTGGTGATGGTGGTGATTGCGTAAc             >  2:141953/1‑93 (MQ=255)
                                                                             cAATTTCGACCACCCAATACCAATCATTGAGAAGCCGACCAGCCCGCCAATCAGCATCAGCAGATATTTGGTGATGGTGGTGATGGCGTAAcc            <  2:2654740/93‑1 (MQ=255)
                                                                              agtttCGACCACCCAATACCAGTCATTGAGAAGCCGACCAGCCCGCCAATCAGCATCAGCAGATATTTGGTGATGGTGGTGATGGCGTAAccc           <  1:3230305/91‑1 (MQ=255)
                                                                              aaTTTCGACCACCCAATACCAATCATTGAGAAGCCGACCAGCCCGCCAATCAGCATCAGCAGATATTTGGTGATGGTGGTGATGGCGTAAccc           >  2:1246169/1‑93 (MQ=255)
                                                                               aTTTCGACCACCCAATACCAATCATTGAGAAGCCGACCAGCCCGCCAATCAGCATCAGCAGATATTTGGTGATGGTGGTGATGGCGTAACCCg          >  2:3579893/1‑93 (MQ=255)
                                                                               aTTTCGACCACCCAATACCAATCATTGAGAAGCCGACCAGCCCGCCAATCAGCATCAGCAGATATTTGGTGATGGTGGTGATGGCGTAACCCg          >  1:1089177/1‑93 (MQ=255)
                                                                                       cACCCAATACCAATCATTGAGAAGCCGACCAGCCCGCCAATCAGCATCAGCAGATATTTGGTGATGGTGGTGATGGCGTAACCCGTACCCGGc  >  1:2455765/1‑93 (MQ=255)
                                                                                          |                                                                                         
AGACCAGAGATAAAGTTGGCGAAAATTTCCTGCAAACCAAAACCGAGACCAACACCGAGCGCGGCAACCAGCCACTGCAATTTCGACCACTCAATACCAATCATTGAGAAGCCGACCAGCCCGCCAATCAGCATCAGCAGATATTTGGTGATGGTGGTGATGGCGTAACCCGTACCCGGC  >  NC_000913/4384172‑4384351

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ATCAGACCAGAGATAAAGTTGGCGAAAATTTCCTGCAAACCAAAACCGAGACCAACACCGAGCGCGGCAACCAGCCACTGCAATTTCGACCACTCAATACCAATCATTGAGAAGCCGACCAGCCCGCCAATCAGCATCAGCAGATATTTGGTGATGGTGGTGATGGCGTAACCCGTACCCGGCGTTA  >  NC_000913/4384169‑4384355
                                                                                             |                                                                                             
ATCAGACCAGAGATAAAGTTGGCGAAAATTTCCTGCAAACCAAAACCGAGACCAACACCGAGCGCGGCAACCAGCCACTGCAATTTCGACCACC                                                                                               >  SRR4302537.3314647/1‑94 (MQ=60)
 TCAGACCAGAGATAAAGTTGGCGAAAATTTCCTGCAAACCAAAACCGAGACCAACACCGAGCGCGGCAACCAGCCACTGCAATTTCGACCACCC                                                                                              >  SRR4302537.4954259/1‑94 (MQ=60)
 TCAGACCAGAGATAAAGTTGGCGAAAATTTCCTGCAAACCAAAACCGAGACCAACACCGAGCGCGGCAACCAGCCACTGCAATTTCGACCACCC                                                                                              <  SRR4302537.3325324/94‑1 (MQ=60)
   AGACCAGAGATAAAGTTGGCGAAAATTTCCTGCAAACCAAAACCGAGACCAACACCGAGCGCGGCAACCAGCCACTGCAATTTCGACCACCCAA                                                                                            >  SRR4302537.974030/1‑94 (MQ=60)
      CCAGAGATAAAGTTGGCGAAAATTTCCTGCAAACCAAAACCGAGACCAACACCGAGCGCGGCAACCAGCCACTGCAATTTCGACCACCCAATAC                                                                                         <  SRR4302537.3696611/94‑1 (MQ=60)
      CCAGAGATAAAGTTGGCGAAAATTTCCTGCAAACCAAAACCGAGACCAACACCGAGCGCGGCAACCAGCCACTGCAATTTCGACCACCCAATAC                                                                                         <  SRR4302537.1077913/94‑1 (MQ=60)
      CCAGAGATAAAGTTGGCGAAAATTTCCTGCAAACCAAAACCGAGACCAACACCGAGAGCGGCAACCAGCCACTGCAATTTCGACCACCCAATAC                                                                                         >  SRR4302537.1292880/1‑94 (MQ=60)
        AGAGATAAAGTTGGCGAAAATTTCCTGCAAACCAAAACCGAGACCAACACCGAGCGCGGCAACCAGCCACTGCAATTTCGACCACCCAATACCA                                                                                       <  SRR4302537.5956119/94‑1 (MQ=60)
          AGATAAAGTTGGCGAAAATTTCCTGCAAACCAAAACCGAGACCAACACCGAGCGCGGCAACCAGCCACTGCAATTTCGACCACCCAATACCAAT                                                                                     <  SRR4302537.6193441/94‑1 (MQ=60)
          AGATAAAGTTGGCGAAAATTTCCTGCAAACCAAAACCGAGACCAACACCGAGCGCGGCAACCAGCCACTGCAATTTCGACCACCCAATACCAAT                                                                                     <  SRR4302537.3917476/94‑1 (MQ=60)
           GATAAAGTTGGCGAAAATTTCCTGCAAACCAAAACCGAGACCAACACCGAGCGCGGCAACCAGCCACTGCAATTTCGACCACCCAATACCAATC                                                                                    >  SRR4302537.19114/1‑94 (MQ=60)
           GATAAAGTTGGCGAAAATTTCCTGCAAACCAAAACCGAGACCAACACCGAGCGCGGCAACCAGCCACTGCAATTTCGACCACCCAATACCAATC                                                                                    >  SRR4302537.4240576/1‑94 (MQ=60)
            ATAAAGTTGGCGAAAATTTCCTGCAAACCAAAACCGAGACCAACACCGAGCGCGGCAACCAGCCACTGCAATTTCGACCACCCAATACCAATCA                                                                                   >  SRR4302537.3429127/1‑94 (MQ=60)
            ATAAAGTTGGCGAAAATTTCCTGCAAACCAAAACCGAGACCAACACCGAGCGCGGCAACCAGCCACTGCAATTTCGACCACCCAATACCAATCA                                                                                   <  SRR4302537.6173502/94‑1 (MQ=60)
            ATAAAGTTGGCGAAAATTTCCTGCAAACCAAAACCGAGACCAACACCGAGCGCGGCAACCAGCCACTGCAATTTCGACCACCCAATACCAATCA                                                                                   >  SRR4302537.3776434/1‑94 (MQ=60)
             TAAAGTTGGCGAAAATTTCCTGCAAACCAAAACCGAGACCAACACCGAGCGCGGCAACCAGCCACTGCAATTTCGACCACCCAATACCAATCAT                                                                                  >  SRR4302537.3173586/1‑94 (MQ=60)
               AAGTTGGCGAAAATTTCCTGCACACCAAAACCGAGACCAACACCGAGCGCGGCAACCAGCCACTGCAATTTCGACCACCCAATACCAATCATTG                                                                                <  SRR4302537.5239536/94‑1 (MQ=60)
               AAGTTGGCGAAAATTTCCTGCAAACCAAAACCGAGACCAACACCGAGCGCGGCAACCAGCCACTGCAATTTCGACCACCCAATACCAATCATTG                                                                                <  SRR4302537.3041662/94‑1 (MQ=60)
                AGTTGGCGAAAATTTCCTGCAAACCAAAACCGAGACCAACACCGAGCGCGGCAACCAGCCACTGCAATTTCGACCACCCAATACCAATCATTGA                                                                               >  SRR4302537.1704977/1‑94 (MQ=60)
                 GTTGGCGAAAATTTCCTGCAAACCAAAACCGAGACCAACACCGAGCGCGGCAACCAGCCACTGCAATTTCGACCACCCAATACCAATCATTGAG                                                                              <  SRR4302537.4833988/94‑1 (MQ=60)
                 GTTGGCGAAAATTTCCTGCAAACCAAAACCGAGACCAACACCGAGCGCGGCAACCAGCCACTGCAATTTCGACCACCCAATACCAATCATTGAG                                                                              <  SRR4302537.5587827/94‑1 (MQ=60)
                 GTTGGCGAAAATTTCCTGCAAACCAAAACCGAGACCAACACCGAGCGCGGCAACCAGCCACTGCAATTTCGACCACCCAATACCAATCATTGAG                                                                              >  SRR4302537.1486564/1‑94 (MQ=60)
                   TGGCGAAAATTTCCTGCAAACCAAAACCGAGACCAACACCGAGCGCGGCAACCAGCCACTGCAATTTCGACCACCCAATACCAATCATTGAGAA                                                                            <  SRR4302537.5823539/94‑1 (MQ=60)
                   TGGCGAAAATTCCCTGCAAACCAAAACCGAGACCAACACCGAGCGCGGCAACCAGCCACTGCAATTTCGACCACCCAATACCAATCATTGAGAA                                                                            <  SRR4302537.205716/94‑1 (MQ=60)
                    GGCGAAAATTTCCTGCAAACCAAAACCGAGACCAACACCGAGCGCGGCAACCAGCCACTGCAATTTCGACCACCCAATACCAATCATTGAGAAG                                                                           <  SRR4302537.1834496/94‑1 (MQ=60)
                     GCGAAAATTTCCTGCAAACCAAAACCGAGACCAACACCGAGCGCGGCAACCAGCCACTGCAATTTCGACCACCCAATACCAATCATTGAGAAGC                                                                          <  SRR4302537.4340962/94‑1 (MQ=60)
                      CGAAATTCTCCTACAAACCAAAACCGAGACCAACCCCGAGCCCGGCAACCAGCCACTGCAATTTCGACCGCCCAATACCAATCATTGAGAAGCC                                                                         <  SRR4302537.3009216/94‑1 (MQ=60)
                      CGAAAATTTCCTGCAAACCAAAACCGAGACCAACACCGAGCGCGGCAACCAGCCACTGCAATTTCGACCACCCAATACCAATCATTGAGAAGCC                                                                         >  SRR4302537.2566194/1‑94 (MQ=60)
                      CGAAAATTTCCTGCAAACCAAAACCGAGACCAACACCGAGCGCGGCAACCAGCCACTGCAATTTCGACCACCCAATACCAATCATTGAGAAGCC                                                                         >  SRR4302537.2063576/1‑94 (MQ=60)
                      CGAAAATTTCCTGCAAACCAAAACCGAGACCAACACCGAGCGCGGCAACCAGCCACTGCAATTTCGACCACCCAATACCAATCATTGAGAAGCC                                                                         <  SRR4302537.6261779/94‑1 (MQ=60)
                       GAAAATTTCCTGCAAACCAAAACCGAGACCAACACCGAGCGCGGCACCCAGCCACTGCAATTTCGACCACCCAATACCAATCATTGAGAAGCCG                                                                        <  SRR4302537.4351015/94‑1 (MQ=60)
                       GAAAATTTCCTGCAAACCAAAACCGAGACCAACACCGAGCGCGGCAACCAGCCACTGCAATTTCGACCACCCAATACCAATCATTGAGAAGCCG                                                                        >  SRR4302537.6061357/1‑94 (MQ=60)
                       GAAAATTTCCTGCAAACCAAAACCGAGACCAACACCGAGCGCGGCAACCAGCCACTGCAATTTCGACCACCCAATACCAATCATTGAGAAGCCG                                                                        <  SRR4302537.440849/94‑1 (MQ=60)
                        AAAATTTCCTGCAAACCAAAACCGAGACCAACACCGAGCGCGGCAACCAGCCACTGCAATTTCGACCACCCAATACCAATCATTGAGAAGCCGA                                                                       <  SRR4302537.846199/94‑1 (MQ=60)
                           ATTTCCTGCAAACCAAAACCGAGACCAACACCGAGCGCGGCAACCAGCCACTGCAATTTCGACCACCCAATACCAATCATTGAGAAGCCGACCA                                                                    >  SRR4302537.5545465/1‑94 (MQ=60)
                           ATTTCCTGCAAACCAAAACCGAGACCAACACCGAGCGCGGCAACCAGCCACTGCAATTTCGACCACCCAATACCAATCATTGAGAAGCCGACCA                                                                    >  SRR4302537.6460075/1‑94 (MQ=60)
                           ATTTCCTGCAAACCAAAACCGAGACCAACACCGAGCGCGGCAACCAGCCACTGCAATTTCGACCACCCAATACCAATCATTGAGAAGCCGACCA                                                                    >  SRR4302537.6186996/1‑94 (MQ=60)
                            TTTCCTGCAAACCAAAACCGAGACCAACACCGAGCGCGGCAACCAGCCACTGCAATTTCGACCACCCAATACCAATCATTGAGAAGCCGACCAG                                                                   <  SRR4302537.3287248/94‑1 (MQ=60)
                             TTCCTGCAAACCAAAACCGAGACCAACCCCGAGCGCGCCAACCAGCCACTGCAATTTCGACCACCCAATACCAATCATTGAGAAGCCGACCAGC                                                                  <  SRR4302537.756045/94‑1 (MQ=60)
                             TTCCTGCAAACCAAAACCGAGACCAACACCGAGCGCGGCAACCAGCCACTGCAATTTCGACCACCCAATACCAATCATTGAGAAGCCGACCAGC                                                                  <  SRR4302537.3536439/94‑1 (MQ=60)
                               CCTGCAAACCAAAACCGAGACCAACACCGAGCGCGGCAACCAGCCACTGCAATTTCGACCACCCAATACCAATCATTGAGAAGCCGACCAGCCC                                                                <  SRR4302537.6150782/94‑1 (MQ=60)
                               CCTGCAAACCAAAACCGAGACCAACACCGAGCGCGGCAACCAGCCACTGCAATTTCGACCACCCAATACCAATCATTGAGAAGCCGACCAGCCC                                                                <  SRR4302537.4240729/94‑1 (MQ=60)
                                CTGCAAACCAAAACCGAGACCAACACCGAGCGCGGCAACCAGCCACTGCAATTTCGACCACCCAATACCAATCATTGAGAAGCCGACCAGCCCG                                                               >  SRR4302537.3443397/1‑94 (MQ=60)
                                  GCAAACCAAAACCGAGACCAACACCGAGCGCGGCAACCAGCCACTGCAATTTCGACCACCCAATACCAATCATTGAGAAGCCGACCAGCCCGCC                                                             <  SRR4302537.170253/94‑1 (MQ=60)
                                   CAAACCAAAACCGAGACCAACACCGAGCGCGGCAACCAGCCACTGCAATTTCGACCACCCAATACCAATCATTGAGAAGCCGACCAGCCCGCCA                                                            >  SRR4302537.5916950/1‑94 (MQ=60)
                                    AAACCAAAACCGAGACCAACACCGAGCGCGGCAACCAGCCACTGCAATTTCGACCACCCAATACCAATCATTGAGAAGCCGACCAGCCCGCCAC                                                           >  SRR4302537.1038447/1‑94 (MQ=60)
                                    AAACCAAAACCGAGACCAACACCGAGCGCGGCAACCAGCCACTGCAATTTCGACCACCCAATACCAATCATTGAGAAGCCGACCAGCCCGCCAA                                                           >  SRR4302537.1825312/1‑94 (MQ=60)
                                     AACCAAAACCGAGACCAACACCGAGCGCGGCAACCAGCCACTGCAATTTCGACCACCCAATACCAATCATTGAGAAGCCGACCAGCCCGCCAAT                                                          <  SRR4302537.5234937/94‑1 (MQ=60)
                                     AACCAAAACCGAGACCAACACCGAGCGCGGCAACCAGCCACTGCAATTTCGACCACCCAATACCAATCATTGAGAAGCCGACCAGCCCGCCAAT                                                          <  SRR4302537.704623/94‑1 (MQ=60)
                                      ACCAAAACCGAGACCAACACCGAGCGCGGCAACCAGCCACTGCAATTTCGACCACCCAATACCAATCATTGAGAAGCCGACCAGCCCGCCAATC                                                         >  SRR4302537.2078062/1‑94 (MQ=60)
                                      ACCAAAACCGAGACCAACACCGAGCGCGGCAACCAGCCACTGCAATTTCGACCACCCAATACCAATCATTGAGAAGCCGACCAGCCCGCCAATC                                                         >  SRR4302537.5000887/1‑94 (MQ=60)
                                      ACCAAAACCGACACCACCACCATGCGCGCCACCCAGCCACTGCAATTTCGACCACCCAATACCAATCATTGAGAAGCCGACCAGCCCGCCAATC                                                         <  SRR4302537.6381467/94‑1 (MQ=60)
                                       CCAAAACCGAGACCAACACCGAGCGCGGCAACCAGCCACTGCAATTTCGACCACCCAATACCAATCATTGAGAAGCCGACCAGCCCGCCAATCA                                                        >  SRR4302537.2569853/1‑94 (MQ=60)
                                       CCAAAACCGAGACCAACACCGAGCGCGGCAACCAGCCACTGCAATTTCGACCACCCAATACCAATCATTGAGAAGCCGACCAGCCCGCCAATCA                                                        <  SRR4302537.40601/94‑1 (MQ=60)
                                        CAAAACCGAGACCAACACCGAGCGCGGCAACCAGCCACTGCAATTTCGACCACCCAATACCAATCATTGAGAAGCCGACCAGCCCGCCAATCAG                                                       >  SRR4302537.4032090/1‑94 (MQ=60)
                                        CAAAACCGAGACCAACACCGAGCGCGGCAACCAGCCACTGCAATTTCGACCACCCAATACCAATCATTGAGAAGCCGACCAGCCCGCCAATCAG                                                       >  SRR4302537.6087604/1‑94 (MQ=60)
                                         AAAACCGAGACCAACACCGAGCGCGGCAACCAGCCACTGCAATTTCGACCACCCAATACCAATCATTGAGAAGCCGACCAGCCCGCCAATCAGC                                                      <  SRR4302537.848380/94‑1 (MQ=60)
                                         AAAACCGAGACCAACACCGAGCGCGGCAACCAGCCACTGCAATTTCGACCACCCAATACCAATCATTGAGAAGCCGACCAGCCCGCCAATCAGC                                                      <  SRR4302537.1063793/94‑1 (MQ=60)
                                          AAACCGAGACCACCCCCGAGCGCGGCACCCAGCCACTGCAATTTCGACCACCCAATACCAATCATTGAGAAGCCGACCAGCCCGCCAATCAGCA                                                     <  SRR4302537.3748928/94‑1 (MQ=60)
                                          AAACCGAGACCAACACCGAGCGCGGCAACCAGCCACTGCAATTTCGACCACCCAATACCAATCATTGAGAAGCCGACCAGCCCGCCAATCAGCA                                                     >  SRR4302537.3084596/1‑94 (MQ=60)
                                           AACCGAGACCAACACCGAGCGCGGCAACCAGCCACTGCAATTTCGACCACCCAATACCAATCATTGAGAAGCCGACCAGCCCGCCAATCAGCAT                                                    <  SRR4302537.6187424/94‑1 (MQ=60)
                                           AACCGAGACCAACACCGAGCGCGGCAACCAGCCACTGCAATTTCGACCACCCAATACCAATCATTGAGAAGCCGACCAGCCCGCCAATCAGCAT                                                    >  SRR4302537.5815995/1‑94 (MQ=60)
                                           AACCGAGACCAACACCGAGCGCGGCAACCAGCCACTGCAATTTCGACCACCCAATACCAATCATTGAGAAGCCGACCAGCCCGCCAATCAGCAT                                                    >  SRR4302537.5900673/1‑94 (MQ=60)
                                           AACCGAGACCAACACCGAGCGCGGCAACCAGCCACTGCAATTTCGACCACCCAATACCAATCATTGAGAAGCCGACCAGCCCGCCAATCAGCAT                                                    >  SRR4302537.5534275/1‑94 (MQ=60)
                                           AACCGAGACCAACACCGAGCGCGGCAACCAGCCACTGCAATTTCGACCACCCAATACCAATCATTGAGAAGCCGACCAGCCCGCCAATCAGCAT                                                    >  SRR4302537.5239833/1‑94 (MQ=60)
                                           AACCGAGACCAACACCGAGCGCGGCAACCAGCCACTGCAATTTCGACCACCCAATACCAATCATTGAGAAGCCGACCAGCCCGCCAATCAGCAT                                                    <  SRR4302537.4947280/94‑1 (MQ=60)
                                             CCGAGACCAACACCGAGCGCGGCAACCAGCCACTGCAATTTCGACCACCCAATACCAATCATTGAGAAGCCGACCAGCCCGCCAATCAACATCA                                                  >  SRR4302537.2224767/1‑94 (MQ=60)
                                              CGAGCCCAACACCGAGCCCGCCACCCAGCCACTGCAATTTCGACCCCCCAATACCAATCATTGAGAAGCCGACCAGCCCGCCAATCAGCATCAG                                                 <  SRR4302537.5581103/94‑1 (MQ=60)
                                               GAGACCAACACCGAGCGCGGCAACCAGCCACTGCAATTTCGACCACCCAATACCAATCAATGAGAAGACGAACAGCCCGCCAATCAGCATCAGC                                                >  SRR4302537.241832/1‑94 (MQ=60)
                                               GAGACCAACACCGAGCGCGCCAACCAGCCACTGCAATTTCGACCACCCAATACCAATCATTGAGAAGCCGACCAGCCCGCCAATCAGCATCAGC                                                <  SRR4302537.703283/94‑1 (MQ=60)
                                                AGACCAACACCGAGCGCGGCAACCAGCCACTGCAATTTCGACCACCCAATACCAATCATTGAGAAGCCGACCAGCCCGCCAATCAGCATCAGCA                                               >  SRR4302537.3880665/1‑94 (MQ=60)
                                                 GACCAACACGGAGCGCGGCAACCAGCCACTGCAATTTCGACCACCCAATACCAATCATTGAGAAGCCGACCAGCCCGCCAATCAGCATCAGCAG                                              <  SRR4302537.2909363/94‑1 (MQ=60)
                                                   CCAACACCGAGCGCGGCAACCAGCCACTGCAATTTCGACCACCCAATACCAATCATTGAGAAGCCGACCAGCCCGCCAATCAGCATCAGCAGAT                                            >  SRR4302537.2162648/1‑94 (MQ=60)
                                                   CCAACACCGAGCGCGGCAACCAGCCACTGCAATTTCGACCACCCAATACCAATCATTGAGAAGCCGACCAGCCCGCCAATCAGCATCAGCAGAT                                            >  SRR4302537.5059117/1‑94 (MQ=60)
                                                    CAACACCGAGCGCGGCAACCAGCCACTGCAATTTCGACCACCCAATACCAATCATTGAGAAGCCGACCAGCCCGCCAATCAGCATCAGCAGATA                                           >  SRR4302537.939229/1‑94 (MQ=60)
                                                    CAACACCGAGCGCGGCAACCAGCCACTGCAATTTCGACCACCCAATACCAATCATTGAGAAGCCGACCAGCCCGCCAATCAGCATCAGCAGATA                                           >  SRR4302537.4599834/1‑94 (MQ=60)
                                                     AACACCGAGCGCGGCAACCAGCCACTGCAATTTCGACCACCCAATACCAATCATTGAGAAGCCGACCAGCCCGCCAATCAGCATCAGCAGATAT                                          <  SRR4302537.3316738/94‑1 (MQ=60)
                                                      ACACCGAGGGCGGCAACCAGCCACTGCAATTTCGACCACCCAATACCAATCATTGAGAAGCCGACCAGCCCGCCAATCAGCATCAGCAGATATT                                         <  SRR4302537.3516622/94‑1 (MQ=60)
                                                       CACCGAGCGCGGCACCCAGCCACTGCAATTTCGACCACCCAATACCAATCATTGAGAAGCCGACCAGCCCGCCAATCAGCATCAGCAGATATTT                                        <  SRR4302537.2422291/94‑1 (MQ=60)
                                                       CACCGAGCGCGGCAACCAGCCACTGCAATTTCGACCACCCAATACCAATCATTGAGAAGCCGACCAGCCCGCCAATCAGCATCAGCAGATATTT                                        <  SRR4302537.1448339/94‑1 (MQ=60)
                                                        CCCGAGCGCGGCAACCAGCCACTGCAATTTCGACCACCCAATACCAATCATTGAGAAGCCGACCAGCCCGCCAATCAGCATCAGCAGATATTTG                                       <  SRR4302537.5446607/94‑1 (MQ=60)
                                                        ACCGAGCGCGGCAACCAGCCACTGCAATTTCGACCACCCAATACCAATCATTGAGAAGCCGACCAGCCCGCCAATCAGCATCAGCAGATATTTG                                       <  SRR4302537.2901469/94‑1 (MQ=60)
                                                         CCGAGCGCGGCAACCAGCCACTGCAATTTCGACCACCCAATACCAATCATTGAGAAGCCGACCAGCCCGCCAATCAGCATCAGCAGATATTTGG                                      >  SRR4302537.2823428/1‑94 (MQ=60)
                                                          CGAGCGCGGCAACCAGCCACTGCAATTTCGACCACCCAATACCAATCATTGAGAAGCCGACCAGCCCGCCAATCAGCATCAGCAGATATTTGGT                                     <  SRR4302537.6073011/94‑1 (MQ=60)
                                                          CGAGCGCGGCAACCAGCCACTGCAATTTCGACCACCCAATACCAATCATTGAGAAGCCGACCAGCCCGCCAATCAGCATCAGCAGATATTTGGT                                     >  SRR4302537.103851/1‑94 (MQ=60)
                                                             GCGAGGCTACCATCCACTGCAATTTCGACCTCCCAATACCAAGCATTGAGAAGCCGACCAGCCCGCCAAGCAGCATCAGCAGATATTTGGTGAT                                  <  SRR4302537.3190170/94‑1 (MQ=60)
                                                              CGCGGCAACCAGCCACTGCAATTTCGACCACCCAATACCAATCATTGAGAAGCCGACCAGCCCGCCAATCAGCATCAGCAGATATTTGGTGATG                                 <  SRR4302537.6511910/94‑1 (MQ=60)
                                                              CGCGGCAACCAGCCACTGCAATTTCGACCACCCAATACCAATCATTGAGAAGCCGACCAGCCCGCCAATCAGCATCAGCAGATATTTGGTGATG                                 >  SRR4302537.4778759/1‑94 (MQ=60)
                                                              CGCGGCAACCAGCCACTGCAATTTCGACCACCCAATACCAATCATTGAGAAGCCGACCAGCCCGCCAATCAGCATCAGCAGATATTTGGTGATG                                 >  SRR4302537.2905338/1‑94 (MQ=60)
                                                              CGCGGCAACCAGCCACTGCAATTTCGACCACCCAATACCAATCATTGAGAAGCCGACCAGCCCGCCAATCAGCATCAGCAGATATTTGGTGATG                                 >  SRR4302537.3787192/1‑94 (MQ=60)
                                                                CGGCAACCAGCCACTGCAATTTCGACCACCCAATACCAATCATTGAGAAGCCGACCAGCCCGCCAATCAGCATCAGCAGATATTTGGTGATGGT                               <  SRR4302537.633643/94‑1 (MQ=60)
                                                                CGGCAACCAGCCACTGCAATTTCGACCACCCAATACCAATCATTGAGAAGCCGACCAGCCCGCCAATCAGCATCAGCAGATATTTGGTGATGGT                               >  SRR4302537.1537011/1‑94 (MQ=60)
                                                                 GGCAACCAGCCACTGCAATTTCGACCACCCAATACCAATCATTGAGAAGCCGACCAGCCCGCCAATCAGCATCAGCAGATATTTGGTGATGGTG                              <  SRR4302537.6292962/94‑1 (MQ=60)
                                                                  GCACCCAGCCACTGCAATTTCGACCACCCAATACCAATCATTGAGAAGCCGACCAGCCCGCCAATCAGCATCAGCAGATATTTGGTGATGGTGG                             <  SRR4302537.2358342/94‑1 (MQ=60)
                                                                  GCAACCAGCCACTGCAATTTCGACCACCCAATACCAATCATTGAGAAGCCGACCAGCCCGCCAATCAGCATCAGCAGATATTTGGTGATGGTGG                             >  SRR4302537.4185568/1‑94 (MQ=60)
                                                                    ACCCAGCCACTGCAATTTCGACCACCCAATACCAATCATTGAGAAGCCGACCAGCCCGCCAATCAGCATCAGCAGATATTTGGTGATGGTGGTG                           <  SRR4302537.2638552/94‑1 (MQ=60)
                                                                    AACCAGCCACTGCAATTTCGACCACCCAATACCAATCATTGAGAAGCCGACCAGCCCGCCAATCAGCATCAGCAGATATTTGGTGATGGTGGTG                           >  SRR4302537.6477676/1‑94 (MQ=60)
                                                                    AACCAGCCACTGCAATTTCGACCACCCAATACCAATCATTGAGAAGCCGACCAGCCCGCCAATCAGCATCAGCAGATATTTGGTGATGGTGGTG                           <  SRR4302537.5762402/94‑1 (MQ=60)
                                                                     ACCAGCCACTGCAATTTCGACCACCCAATACCAATCATTGAGAAGCCGACCAGCCCGCCAATCAGCATCAGCAGATATTTGGTGATGGTGGTGA                          <  SRR4302537.3079146/94‑1 (MQ=60)
                                                                     ACCAGCCACTGCAATTTCGACCACCCAATACCAATCATTGAGAAGCCGACCAGCCCGCCAATCAGCATCAGCAGATATTTGGTGATGGTGGTGA                          >  SRR4302537.665750/1‑94 (MQ=60)
                                                                      CCAGCCACTGCAATTTCGACCACCCAATACCAATCATTGAGAAGCCGACCAGCCCGCCAATCAGCATCAGCAGATATTTGGTGATGGTGGTGAT                         >  SRR4302537.6219519/1‑94 (MQ=60)
                                                                      CCAGCCACTGCAATTTCGACCACCCAATACCAATCATTGAGAAGCCGACCAGCCCGCCAATCAGCATCAGCAGATATTTGGTGATGGTGGTGAT                         >  SRR4302537.325379/1‑94 (MQ=60)
                                                                       CAGCCACTGCAATTTCGACCACCCAATACCAATCATTGAGAAGCCGACCAGCCCGCCAATCAGCATCAGCAGATATTTGGTGATGGTGGTGATG                        >  SRR4302537.164996/1‑94 (MQ=60)
                                                                       CAGCCACTGCAATTTCGACCACCCAATACCAATCATTGAGAAGCCGACCAGCCCGCCAATCAGCATCAGCAGATATTTGGTGATGGTGGTGATG                        <  SRR4302537.4773642/94‑1 (MQ=60)
                                                                        AGCCACTGCAATTTCGACCACCCAATACCAATCATTGAGAAGCCGACCAGCCCGCCAATCAGCATCAGCAGATATTTGGTGATGGTGGTGATGG                       >  SRR4302537.51515/1‑94 (MQ=60)
                                                                         GCCACTGCAATTTCGACCACCCAATACCAATCATTGAGAAGCCGACCAGCCCGCCAATCAGCATCAGCAGATATTTGGTGATGGTGGTGATGGC                      >  SRR4302537.4968218/1‑94 (MQ=60)
                                                                         GCCACTGCAATTTCGACCACCCAATACCAATCATTGAGAAGCCGACCAGCCCGCCAATCAGCATCAGCAGATATTTGGTGATGGTGGTGATGGC                      >  SRR4302537.4739432/1‑94 (MQ=60)
                                                                           CACTGCAATTTCGACCACCCAATACCAATCATTGAGAAGCCGACCAGCCCGCCAATCAGCATCAGCAGATATTTGGTGATGGTGGTGATGGCGT                    >  SRR4302537.5141889/1‑94 (MQ=60)
                                                                            ACTGCAATTTCGACCACCCAATACCAATCATTGAGAAGCCGACCAGCCCGCCAATCAGCATCAGCAGATATTTGGTGATGGTGGTGATGGCGTA                   >  SRR4302537.626354/1‑94 (MQ=60)
                                                                             CTGCAATTTCGACCACCCAATACCAATCATTGAGAAGCCGACCAGCCCGCCAATCAGCATCAGCAGATATTTGGTGATGGTGGTGATGGCGTAA                  >  SRR4302537.6521141/1‑94 (MQ=60)
                                                                              TGCAATTTCGACCACCCAATACCAATCATTGAGAAGCCGACCAGCCCGCCAATCAGCATCAGCAGATATTTGGTGATGGTGGTGATGGCGTAAC                 >  SRR4302537.4177689/1‑94 (MQ=60)
                                                                              TGCAATTTCGACCACCCAATACCAATCATTGAGAAGCCGACCAGCCCGCCAATCAGCATCAGCAGATATTTGGTGATGGTGGTGATGGCGTAAC                 >  SRR4302537.891126/1‑94 (MQ=60)
                                                                                CAATTTCGACCACCCAATACCAATCATTGAGAAGCCGACCAGCCCGCCAATCAGCATCAGCAGATATTTGGTGATGGTGGTGATGGCGTAACCC               >  SRR4302537.5793595/1‑94 (MQ=60)
                                                                                 AGTTTCGACCACCCAATACCAGTCATTGAGAAGCCGACCAGCCCGCCAATCAGCATCAGCAGATATTTGGTGATGGTGGTGATGGCGTAACCCG              <  SRR4302537.3253072/94‑1 (MQ=60)
                                                                                 AATTTCGACCACCCAATACCAATCATTGAGAAGCCGACCAGCCCGCCAATCAGCATCAGCAGATATTTGGTGATGGTGGTGATGGCGTAACCCG              <  SRR4302537.5826470/94‑1 (MQ=60)
                                                                                 AATTTCGACCACCCAATACCAATCATTGAGAAGCCGACCAGCCCGCCAATCAGCATCAGCAGATATTTGGTGATGGTGGTGATGGCGTAACCCG              >  SRR4302537.1096701/1‑94 (MQ=60)
                                                                                  ATTTCGACCACCCAATACCAATCATTGAGAAGCCGACCAGCCCGCCAATCAGCATCAGCAGATATTTGGTGATGGTGGTGATGGCGTAACCCGT             >  SRR4302537.4688851/1‑94 (MQ=60)
                                                                                  ATTTCGACCACCCAATACCAATCATTGAGAAGCCGACCAGCCCGCCAATCAGCATCAGCAGATATTTGGTGATGGTGGTGATGGCGTAACCCGT             >  SRR4302537.5030565/1‑94 (MQ=60)
                                                                                    TTCGACCACCCAATACCAATCATTGAGAAGCCGACCAGCCCGCCAATCAGCATCAGCAGATATTTGGTGATGGTGGTGATGGGGTAACCCGTAC           <  SRR4302537.4430875/94‑1 (MQ=60)
                                                                                    TTCGACCACCCAATACCAATCATTGAGAAGCCGACCAGCCCGCCAATCAGCATCAGCAGATATTTGGTGATGGTGGTGATGGCGTAACCCGTAC           >  SRR4302537.5303179/1‑94 (MQ=60)
                                                                                      CGACCACCCAATACCAATCATTGAGAAGCCGACCAGCCCGCCAATCAGCATCAGCAGATATTTGGTGATGGTGGTGATGGCGTAACCCGTACCC         <  SRR4302537.4053148/94‑1 (MQ=60)
                                                                                       GACCACCCAATACCAATCATTGAGAAGCCGACCAGCCCGCCAATCAGCATCAGCAGATATTTGGTGATGGTGGTGATGGCGTAACCCGTACCCG        <  SRR4302537.3570668/94‑1 (MQ=60)
                                                                                         CCACCCAATACCAATCATTGAGAAGCCGACCAGCCCGCCAATCAGCATCAGCAGATATTTGGTGATGGTGGTGATGGCGTAACCCGTACCCGGC      >  SRR4302537.2473038/1‑94 (MQ=60)
                                                                                          CACCCAATACCAATCATTGAGAAGCCGACCAGCCCGCCAATCAGCATCAGCAGATATTTGGTGATGGTGGTGATGGCGTAACCCGTACCCGGCG     >  SRR4302537.4494163/1‑94 (MQ=60)
                                                                                            CCCAATACCAATCATTGAGAAGCCGACCAGCCCGCCAATCAGCATCAGCAGATATTTGGTGATGGTGGTGATGGCGTAACCCGTACCCGGCGTT   >  SRR4302537.3192006/1‑94 (MQ=60)
                                                                                             CCAATACCAATCATTGAGAAGCCGACCAGCCCGCCAATCAGCATCAGCAGATATTTGGTGATGGTGGTGATGGCGTAACCCGTACCCGGCGTTA  >  SRR4302537.151946/1‑94 (MQ=60)
                                                                                             CCAATACCAATCATTGAGAAGCCGACCAGCCCGCCAATCAGCATCAGCAGATATTTGGTGATGGTGGTGATGGCGTAACCCGTACCCGGCGTTA  >  SRR4302537.342482/1‑94 (MQ=60)
                                                                                             CCAATACCAATCATTGAGAAGCCGACCAGCCCGCCAATCAGCATCAGCAGATATTTGGTGATGGTGGTGATGGCGTAACCCGTACCCGGCGTTA  <  SRR4302537.2836520/94‑1 (MQ=60)
                                                                                             |                                                                                             
ATCAGACCAGAGATAAAGTTGGCGAAAATTTCCTGCAAACCAAAACCGAGACCAACACCGAGCGCGGCAACCAGCCACTGCAATTTCGACCACTCAATACCAATCATTGAGAAGCCGACCAGCCCGCCAATCAGCATCAGCAGATATTTGGTGATGGTGGTGATGGCGTAACCCGTACCCGGCGTTA  >  NC_000913/4384169‑4384355

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑