Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 882 254.4 12957588 98.0% 12698436 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,464,261 T→C D187G (GAC→GGC)  treR ← TreR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,464,2610TC97.5% 677.8 / 3.1 205D187G (GAC→GGC) treRTreR
Reads supporting (aligned to +/- strand):  ref base T (4/1);  new base C (106/93);  total (111/94)
Fisher's exact test for biased strand distribution p-value = 3.77e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.80e-01

Only 100 of 218 total aligned reads displayed.

TAGCAAGCCCTGGCAGGGCGGCAACGGGATGCAGTTTATGCGCTTTGCAGAACGCCAGGTAGGCTTCGTGACGTCGCTTACCGGTTGTCACGTCACTGTGCGGCACGCCGAGATAACTGATATTACGATGCCCCTGGTCATACAGCCGTTGCATCAGGATTTTGATTGCCCCTTCGTCGTCATAA  >  NC_000913/4464169‑4464353
                                                                                            |                                                                                            
   cAAGCCCTGGCAGGGCGGCAACGGGATGCAGTTTATGCGCTTTGCAGAACGCCAGGTAGGCTTCGTGACGTCGCTTACCGGTTggcacgccac                                                                                           <  1:1860822/93‑1 (MQ=255)
     aGCCCTGGCAGGGCGGCAACGGGATGCAGTTTATGCGCTTTGCAGAACGCCAGGTAGGCTTCGTGACGTCGCTTACCGGTTGTCACGCCACtg                                                                                         >  1:3585085/1‑93 (MQ=255)
     aGCCCTGGCAGGGCGGCAACGGGATGCAGTTTATGCGCTTTGCAGAACGCCAGGTAGGCTTCGTGACGTCGCTTACCGGTTGTCACGCCACtg                                                                                         >  2:4072477/1‑93 (MQ=255)
      gCCCTGGCAGGGCGGCAACGGGATGCAGTTTATGCGCTTTGCAGAACGCCAGGTAGGCTTCGTGACGTCGCTTACCGGTTGTCACGCCACtgt                                                                                        >  1:5735639/1‑93 (MQ=255)
      gCCCTGGCAGGGCGGCAACGGGATGCAGTTTATGCGCTTTGCAGAACGCCAGGTAGGCTTCGTGACGTCGCTTACCGGTTGTCACGCCACtgt                                                                                        >  1:4144688/1‑93 (MQ=255)
      gCCCTGGCAGGGCGGCAACGGGATGCAGTTTATGCGCTTTGCAGAACGCCAGGTAGGCTTCGTGACGTCGCTTACCGGTTGTCACGCCACtgt                                                                                        <  1:2734769/93‑1 (MQ=255)
        ccTGGCAGGGCGGCAACGGGATGCAGTTTATGCGCTTTGCAGAACGCCAGGTAGGCTTCGTGACGTCGCTTACCGGTTGTCACGCCACTGTGc                                                                                      >  2:423075/1‑93 (MQ=255)
        ccTGGCAGGGCGGCAACGGGATGCAGTTTATGCGCTTTGCAGAACGCCAGGTAGGCTTCGTGACGTCGCTTACCGGTTGTCACGCCACTGTGc                                                                                      >  2:6297877/1‑93 (MQ=255)
        ccTGGCAGGGCGGCAACGGGATGCAGTTTATGCGCTTTGCAGAACGCCAGGTAGGCTTCGTGACGTCGCTTACCGGTTGTCACGCCACTGTGc                                                                                      <  1:77465/93‑1 (MQ=255)
        ccTGGCAGGGCGGCAACGGGATGCAGTTTATGCGCTTTGCAGAACGCCAGGTAGGCTTCGTGACGTCGCTTACCGGTTGTCACGCCACTGTGc                                                                                      >  1:4349912/1‑93 (MQ=255)
         cTGGCAGGGCGGCAACGGGATGCAGTTTATGCGCTTTGCAGAACGCCAGGTAGGCTTCGTGACGTCGCTTACCGGTTGTCACGCCACTGTGCg                                                                                     >  2:4244773/1‑93 (MQ=255)
         cTGGCAGGGCGGCAACGGGATGCAGTTTATGCGCTTTGCAGAACGCCAGGTAGGCTTCGTGACGTCGCTTACCGGTTGTCACGCCACTGTGCg                                                                                     >  2:56722/1‑93 (MQ=255)
          tGGCAGGGCGGCAACGGGATGCAGTTTATGCGCTTTGCAGAACGCCAGGTAGGCTTCGTGACGTCGCTTACCGGTTGTCACGTCACTGTGCgg                                                                                    >  2:5991783/1‑93 (MQ=255)
          tGGCAGGGCGGCAACGGGATGCAGTTTATGCGCTTTGCAGAACGCCAGGTAGGCTTCGTGACGTCGCTTACCGGTTGTCACGCCACTGTGCgg                                                                                    >  1:4385520/1‑93 (MQ=255)
          tGGCAGGGCGGCAACGGGATGCAGTTTATGCGCTTTGCAGAACGCCAGGTAGGCTTCGTGACGTCGCTTACCGGTTGTCACGCCACTGTGCgg                                                                                    <  2:4520565/93‑1 (MQ=255)
           ggCAGGGCGGCAACGGGATGCAGTTTATGCGCTTTGCAGAACGCCAGGTAGGCTTCGTGACGTCGCTTACCGGTTGTCACGCCACTGTGCGGc                                                                                   <  1:933614/93‑1 (MQ=255)
              aGGGCGGCAACGGGATGCAGTTTATGCGCTTTGCAGAACGCCAGGTAGGCTTCGTGACGTCGCTTACCGGTTGTCACGCCACTGTGCGGCACg                                                                                <  1:1449496/93‑1 (MQ=255)
               gggCGGCAACGGGATGCAGTTTATGCGCTTTGCAGAACGCCAGGTAGGCTTCGTGACGTCGCTTACCGGTTGTCACGCCACTGTGCGGCACGc                                                                               >  2:6061647/1‑93 (MQ=255)
               gggCGGCAACGGGATGCAGTTTATGCGCTTTGCAGAACGCCAGGTAGGCTTCGTGACGTCGCTTACCGGTTGTCACGCCACTGTGCGGCACGc                                                                               >  2:477955/1‑93 (MQ=255)
                 gcggcAACGGGATGCAGTTTATGCGCTTTGCAGAACGCCAGGTAGGCTTCGTGACGTCGCTTACCGGTTGTCACGCCACTGTGCGGCACGCCg                                                                             >  2:5484180/1‑93 (MQ=255)
                  cggcAACGGGATGCAGTTTATGCGCTTTGCAGAACGCCAGGTAGGCTTCGTGACGTCGCTTACCGGTTGTCACGCCACTGTGCGGCACGCCga                                                                            <  2:3670238/93‑1 (MQ=255)
                  cggcAACGGGATGCAGTTTATGCGCTTTGCAGAACGCCAGGTAGGCTTCGTGACGTCGCTTACCGGTTGTCACGCCACTGTGCGGCACGCCga                                                                            <  2:1014592/93‑1 (MQ=255)
                   ggcAACGGGATGCAGTTTATGCGCTTTGCAGAACGCCAGGTAGGCTTCGTGACGTCGCTTACCGGTTGTCACGCCACTGTGCGGCACGCCgag                                                                           >  2:4954642/1‑93 (MQ=255)
                   ggcAACGGGATGCAGTTTATGCGCTTTGCAGAACGCCAGGTAGGCTTCGTGACGTCGCTTACCGGTTGTCACGCCACTGTGCGGCACGCCgag                                                                           <  2:2727312/93‑1 (MQ=255)
                      aaCGGGATGCAGTTTATGCGCTTTGCAGAACGCCAGGTAGGCTTCGTGACGTCGCTTACCGGTTGTCACGCCACTGTGCGGCACGCCGAGATa                                                                        >  2:2374853/1‑93 (MQ=255)
                      aaCGGGATGCAGTTTATGCGCTTTGCAGAACGCCAGGTAGGCTTCGTGACGTCGCTTACCGGTTGTCACGCCACTGTGCGGCACGCCGAGATa                                                                        <  1:3013023/93‑1 (MQ=255)
                       aCGGGATGCAGTTTATGCGCTTTGCAGAACGCCAGGTAGGCTTCGTGACGTCGCTTACCGGTTGTCACGCCACTGTGCGGCACGCCGAGATaa                                                                       >  1:2166220/1‑93 (MQ=255)
                       aCGGGATGCAGTTTATGCGCTTTGCAGAACGCCAGGTAGGCTTCGTGACGTCGCTTACCGGTTGTCACGCCACTGTGCGGCACGCCGAGATaa                                                                       <  1:1265709/93‑1 (MQ=255)
                        cGGGATGCAGTTTATGCGCTTTGCAGAACGCCAGGTAGGCTTCGTGACGTCGCTTACCGGTTGTCACGCCACTGTGCGGCACGCCGAGATAAc                                                                      <  2:2765355/93‑1 (MQ=255)
                         gggATGCAGTTTATGCGCTTTGCAGAACGCCAGGTAGGCTTCGTGACGTCGCTTACCGGTTGTCACGCCACTGTGCGGCACGCCGAGATAACt                                                                     >  2:211286/1‑93 (MQ=255)
                         gggATGCAGTTTATGCGCTTTGCAGAACGCCAGGTAGGCTTCGTGACGTCGCTTACCGGTTGTCACGCCACTGTGCGGCACGCCGAGATAACt                                                                     >  2:3486273/1‑93 (MQ=255)
                           gATGCAGTTTATGCGCTTTGCAGAACGCCAGGTAGGCTTCGTGACGTCGCTTACCGGTTGTCACGCCACTGTGCGGCACGCCGAGATAACTGa                                                                   >  1:1374295/1‑93 (MQ=255)
                           gATGCAGTTTATGCGCTTTGCAGAACGCCAGGTAGGCTTCGTGACGTCGCTTACCGGTTGTCACGCCACTGTGCGGCACGCCGAGATAACTGa                                                                   <  1:1218503/93‑1 (MQ=255)
                           gATGCAGTTTATGACCTCTGCAGAACGCCAGGTAGGCCTCGTGACGGCGCTTACCGGTTGACACGGCACCGTGCGGGACGGCGTGAGAACTgt                                                                   >  2:4391951/1‑92 (MQ=255)
                            aTGCAGTTTATGCGCTTTGCAGAACGCCAGGTAGGCTTCGTGTCGTCGCTTACCGGTTGTCACGCCACTGTGCGGCACGCCGAGATAACTGat                                                                  >  1:3461990/1‑93 (MQ=255)
                             tGCAGTTTATGCGCTTTGCAGAACGCCAGGTAGGCTTCGTGACGTCGCTTACCGGTTGTCACGCCACTGTGCGGCACGCCGAGATAACTGata                                                                 >  2:5172844/1‑93 (MQ=255)
                                 gTTTATGCGCTTTGCAGAACGCCAGGTAGGCTTCGTGACGTCGCTTACCGGTTGTCACGCCACTGTGCGGCACGCCGAGATAACTGATATTAc                                                             <  2:3469860/93‑1 (MQ=255)
                                   ttATGCGCTTTGCAGAACGCCAGGTAGGCTTCGTGACGTCGCTTACCGGTTGTCACGCCACTGTGCGGCACGCCGAGATAACTGATATTACGa                                                           <  1:1141195/93‑1 (MQ=255)
                                    tATGCGCTTTGCAGAACGCCAGGTAGGCTTCGTGACGTCGCTTACCGGTTGTCACGCCACTGTGCGGCACGCCGAGATAACTGATATTACGAt                                                          <  1:5374094/93‑1 (MQ=255)
                                    tATGCGCTTTGCAGAACGCCAGGTAGGCTTCGTGACGTCGCTTACCGGTTGTCACGCCACTGTGCGGCACGCCGAGATAACTGATATTACGAt                                                          >  1:3352659/1‑93 (MQ=255)
                                     aTGCGCTTTGCAGAACGCCAGGTAGGCTTCGTGACGTCGCTTACCGGTTGTCACGCCACTGTGCGGCACGCCGAGATAACTGATATTACGATg                                                         >  1:2436803/1‑93 (MQ=255)
                                     aTGCGCTTTGCAGAACGACAGGTAGGCTTCGTGACGTCGCTTACCGGTTGTCACGCCACTGGGCGGCACGCCGCGATAACTGATATTACGATg                                                         >  1:1066972/1‑93 (MQ=255)
                                        cgcTTTGCAGAACGCCAGGTAGGCTTCGTGACGTCGCTTACCGGTTGTCACGCCACTGTGCGGCACGCCGAGATAACTGATATTACGATGccc                                                      <  2:3555080/93‑1 (MQ=255)
                                         gcTTTGCAGAACGCCAGGTAGGCTTCGTGACGTCGCTTACCGGTTGTCACGCCACTGTGCGGCACGCCGAGATAACTGATATTACGATGcccc                                                     <  1:1227886/93‑1 (MQ=255)
                                          cTTTGCAGAACGCCAGGTAGGCTTCGTGACGTCGCTTACCGGTTGTCACGCCACTGTGCGGCACGCCGAGATAACTGATATTACGATGCCCCt                                                    >  1:4893474/1‑93 (MQ=255)
                                          cTTTGCAGAACGCCAGGTAGGCTTCGTGACGTCGCTTACCGGTTGTCACGCCACTGTGCGGCACGCCGAGATAACTGATATTACGATGCCCCt                                                    <  2:5056453/93‑1 (MQ=255)
                                          cTTTGCAGAACGCCAGGTAGGCTTCGTGACGTCGCTTACCGGTTGTCACGCCACTGTGCGGCACGCCGAGATAACTGATATTACGATGCCCCt                                                    >  1:2047720/1‑93 (MQ=255)
                                           tttGCAGAACGCCAGGTAGGCTTCGTGACGTCGCTTACCGGTTGTCACGTCACTGTGCGGCACGCCGAGATAACTGATATTACGATGCCCCTg                                                   >  1:6263608/1‑93 (MQ=255)
                                           tttGCAGAACGCCAGGTAGGCTTCGTGACGTCGCTTACCGGTTGTCACGCCACTGTGCGGCACGCCGAGATAACTGATATTACGATGCCCCTg                                                   <  2:426004/93‑1 (MQ=255)
                                           tttGCAGAACGCCAGGTAGGCTTCGTGACGTCGCTTACCGGTTGTCACGCCACTGTGCGGCACGCCGAGATAACTGATATTACGATGCCCCTg                                                   <  2:1497764/93‑1 (MQ=255)
                                           tttGCAGAACGCCAGGTAGGCTTCGTGACGTCGCTTACCGGTTGTCACGCCACTGTGCGGCACGCCGAGATAACTGATATTACGATGCCCCTg                                                   >  2:1808665/1‑93 (MQ=255)
                                             tGCAGAACGCCAGGTAGGCTTCGTGACGTCGCTTACCGGTTGTCACGCCACTGTGCGGCACGCCGAGATAACTGATATTACGATGCCCCTGGt                                                 <  2:1551605/93‑1 (MQ=255)
                                              gCAGAACGCCAGGTAGGCTTCGTGACGTCGCTTACCGGTTGTCACGCCACTGTGCGGCACGCCGAGATAACTGATATTACGATGCCCCTGGTc                                                >  2:5345679/1‑93 (MQ=255)
                                               cAGAACGCCAGGTAGGCTTCGTGACGTCGCTTACCGGTTGTCACGCCACTGTGCGGCACGCCGAGATAACTGATATTACGATGCCCCTGGTCa                                               <  2:5131651/93‑1 (MQ=255)
                                                aGAACGCCAGGTAGGCTTCGTGACGTCGCTTACCGGTTGTCACGCCACTGTGCGGCACGCCGAGATAACTGATATTACGATGCCCCTGGTCAt                                              >  1:6332306/1‑93 (MQ=255)
                                                     gCCAGGTAGGCTTCGTGACGTCGCTTACCGGTTGTCACGCCACTGTGCGGCACGCCGAGATAACTGATATTACGATGCCCCTGGTCATACAGc                                         <  1:3405571/93‑1 (MQ=255)
                                                     gCCAGGTAGGCTTCGTGACGTCGCTTACCGGTTGTCACGCCACTGTGCGGCACGCCGAGATAACTGATATTACGATGCCCCTGGTCATACAGc                                         >  2:30187/1‑93 (MQ=255)
                                                      ccAGGTAGGCTTCGTGACGTCGCTTACCGGTTGTCACGCCACTGTGCGGCACGCCGAGATAACTGATATTACGATGCCCCTGGTCATACAGcc                                        >  2:3769906/1‑93 (MQ=255)
                                                        aGGTAGGCTTCGTGACGTCGCTTACCGGTTGTCACGCCACTGTGCGGCACGCCGAGATAACTGATATTACGATGCCCCTGGTCATACAGCCGt                                      >  2:4495480/1‑93 (MQ=255)
                                                         ggTAGGCTTCGTGACGTCGCTTACCGGTTGTCACGCCACTGTGCGGCACGCCGAGATAACTGATATTACGATGCCCCTGGTCATACAGCCGtt                                     >  1:2817352/1‑93 (MQ=255)
                                                           tAGGCTTCGTGACGTCGCTTACCGGTTGTCACGCCACTGTGCGGCACGCCGAGATAACTGATATTACGATGCCCCTGGTCATACAGCCGTTGc                                   >  1:3980293/1‑93 (MQ=255)
                                                            aGGCTTCGTGACGTCGCTTACCGGTTGTCACGCCACTGTGCGGCACGCCGAGATAACTGATATTACGATGCCCCTGGTCATACAGCCGTTGCa                                  <  1:3100606/93‑1 (MQ=255)
                                                            aGGCTTCGTGACGTCGCTTACCGGTTGTCACGCCACTGTGCGGCACGCCGAGATAACTGATATTACGATGCCCCTGGTCATACAGCCGTTGCa                                  >  1:6466039/1‑93 (MQ=255)
                                                             ggCTTCGTGACGTCGCTTACCGGTTGTCACGCCACTGTGCGGCACGCCGAGATAACTGATATTACGATGCCCCTGGTCATACAGCCGTTGCAt                                 <  1:1967243/93‑1 (MQ=255)
                                                             ggCTTCGTGACGTCGCTTACCGGTTGTCACGCCACTGTGCGGCACGCCGAGATAACTGATATTACGATGCCCCTGGTCATACAGCCGTTGCAt                                 <  2:817723/93‑1 (MQ=255)
                                                              gCTTCGTGACGTCGCTTACCGGTTGTCACGCCACTGTGCGGCACGCCGAGATAACTGATATTACGATGCCCCTGGTCATACAGCCGTTGCATc                                <  2:931314/93‑1 (MQ=255)
                                                               cTTCGTGACGTCGCTTACCGGTTGTCACGCCACTGTGCGGCACGCCGAGATAACTGATATTACGATGCCCCTGGTCATACAGCCGTTGCATCa                               <  2:650914/93‑1 (MQ=255)
                                                               cTTCGTGACGTCGCTTACCGGTTGTCACGCCACTGTGCGGCACGCCGAGATAACTGATATTACGATGCCCCTGGTCATACAGCCGTTGCATCa                               <  2:3090132/93‑1 (MQ=255)
                                                                ttCGTGACGTCGCTTACCGGTTGTCACGCCACTGTGCGGCACGCCGAGATAACTGATATTACGATGCCCCTGGTCATACAGCCGTTGCATCAg                              >  1:5401216/1‑93 (MQ=255)
                                                                ttCGTGACGTCGCTTACCGGTTGTCACGCCACTGTGCGGCACGCCGAGATAACTGATATTACGATGCCCCTGGTCATACAGCCGTTGCATCAg                              >  1:5321910/1‑93 (MQ=255)
                                                                ttCGTGACGTCGCTTACCGGTTGTCACGCCACTGTGCGGCACGCCGAGATAACTGATATTACGATGCCCCTGGTCATACAGCCGTTGCATCAg                              >  1:3035919/1‑93 (MQ=255)
                                                                 tCGTGACGTCGCTTACCGGTTGTCACGCCACTGTGCGGCACGCCGAGATAACTGATATTACGATGCCCCTGGTCATACAGCCGTTGCATCAgg                             >  1:349410/1‑93 (MQ=255)
                                                                  cGTGACGTCGCTTACTGGTTGTCACGCCACTGTGCGGCACGCCGAGATAACTGATATTACGATGCCCCTGGTCATACAGCCGTTGCATCAGGa                            <  2:2847514/93‑1 (MQ=255)
                                                                  cGTGACGTCGCTTACCGGTTGTCACGCCACTGTGCGGCACGCCGAGATAACTGATATTACGATGCCCCTGGTCATACAGCCGTTGCATCAGGa                            <  2:873779/93‑1 (MQ=255)
                                                                   gTGACGTCGCTTACCGGTTGTCACGCCACTGTGCGGCACGCCGAGATAACTGATATTACGATGCCCCTGGTCATACAGCCGTTGCATCAGGAt                           >  2:5277927/1‑93 (MQ=255)
                                                                   gTGACGTCGCTTACCGGTTGTCACGCCACTGTGCGGCACGCCGAGATAACTGATATTACGATGCCCCTGGTCATACAGCCGTTGCATCAGGAt                           >  1:330725/1‑93 (MQ=255)
                                                                   gTGACGTCGCTTACCGGTTGTCACGCCACTGTGCGGCACGCCGAGATAACTGATATTACGATGCCCCTGGTCATACAGCCGTTGCATCAGGAt                           <  1:4694188/93‑1 (MQ=255)
                                                                   gTGACGTCGCTTACCGGTTGTCACGCCACTGTGCGGCACGCCGAGATAACTGATATTACGATGCCCCTGGTCATACAGCCGTTGCATCAGGAt                           <  1:2253355/93‑1 (MQ=255)
                                                                    tGACGTCGCTTACCGGTTGTCACGCCACTGTGCGGCACGCCGAGATAACTGATATTACGATGCCCCTGGTCATACAGCCGTTGCATCAGGAtt                          >  1:5438008/1‑93 (MQ=255)
                                                                    tGACGTCGCTTACCGGTTGTCACGCCACTGTGCGGCACGCCGAGATAACTGATATTACGATGCCCCTGGTCATACAGCCGTTGCATCAGGAtt                          >  1:2400224/1‑93 (MQ=255)
                                                                     gACGTCGCTTACCGGTTGTCACGCCACTGTGCGGCACGCCGAGATAACTGATATTACGATGCCCCTGGTCATACAGCCGTTGCATCAGGAttt                         <  2:2162913/93‑1 (MQ=255)
                                                                     gACGTCGCTTACCGGTTGTCACGCCACTGTGCGGCACGCCGAGATAACTGATATTACGATGCCCCTGGTCATACAGCCGTTGCATCAGGAttt                         <  2:4902390/93‑1 (MQ=255)
                                                                      aCGTCGCTTACCGGTTGTCACGCCACTGTGCGGCACGCCGAGATAACTGATATTACGATGCCCCTGGTCATACAGCCGTTGCATCAGGAtttt                        <  2:1282130/93‑1 (MQ=255)
                                                                      aCGTCGCTTACCGGTTGTCACGCCACTGTGCGGCACGCCGAGATAACTGATATTACGATGCCCCTGGTCATACAGCCGTTGCATCAGGAtttt                        >  1:5237791/1‑93 (MQ=255)
                                                                        gTCGCTTACCGGTTGTCACGCCACTGTGCGGCACGCCGAGATAACTGATATTACGATGCCCCTGGTCATACAGCCGTTGCATCAGGATTTTGa                      <  2:3385344/93‑1 (MQ=255)
                                                                         tCGCTTACCGGTTGTCACGCCACTGTGCGGCACGCCGAGATAACTGATATTACGATGCCCCTGGTCATACAGCCGTTGCATCAGGATTTTGAt                     <  1:4575006/93‑1 (MQ=255)
                                                                           gCTTACCGGTTGTCACGCCACTGTGCGGCACGCCGAGATAACTGATATTACGATGCCCCTGGTCATACAGCCGTTGCATCAGGATTTTGATTg                   <  2:1612535/93‑1 (MQ=255)
                                                                             ttACCGGTTGTCACGCCACTGTGCGGCACGCTGAGATAACTGATATTACGATGCCCCTGGTCATACAGCCGTTGCATCAGGATTTTGATTGcc                 <  2:6053814/93‑1 (MQ=255)
                                                                             ttACCGGTTGTCACGCCACTGTGCGGCACGCCGAGATAACTGATATTACGATGCCCCTGGTCATACAGCCGTTGCATCAGGATTTTGATTGcc                 <  1:2905677/93‑1 (MQ=255)
                                                                               aCCGGTTGTCACGCCACTGTGCGGCACGCCGAGATAACTGATATTACGATGCCCCTGGTCATACAGCCGTTGCATCAGGATTTTGATTGcccc               <  2:2598221/93‑1 (MQ=255)
                                                                               aCCGGTTGTCACGCCACTGTGCGGCACGCCGAGATAACTGATATTACGATGCCCCTGGTCATACAGCCGTTGCATCAGGATTTTGATTGcccc               <  1:4678819/93‑1 (MQ=255)
                                                                                ccGGTTGTCACGCCACTGTGCGGCACGCCGAGATAACTGATATTACGATGCCCCTGGTCATACAGCCGTTGCATCAGGATTTTGATTGCCCCt              <  2:1529603/93‑1 (MQ=255)
                                                                                ccGGTTGTCACGCCACTGTGCGGCACGCCGAGATAACTGATATTACGATGCCCCTGGTCATACAGCCGTTGCATCAGGATTTTGATTGCCCCt              >  2:2459178/1‑93 (MQ=255)
                                                                                 cGGTTGTCACGCCACTGTGCGGCACGCCGAGATAACTGATATTACGATGCCCCTGGTCATACAGCCGTTGCATCAGGATTTTGATTGCCCCtt             <  2:1681869/93‑1 (MQ=255)
                                                                                   gTTGTCACGCCACTGTGCGGCACGCCGAGATAACTGATATTACGATGCCCCTGGTCATACAGCCGTTGCATCAGGATTTTGATTGCCCCTtcg           >  1:946445/1‑93 (MQ=255)
                                                                                    ttGTCACGCCACTGTGCGGCACGCCGAGATAACTGATATTACGATGCCCCTGGTCATACAGCCGTTGCATCAGGATTTTGATTGCCCCTtcgt          <  1:377258/93‑1 (MQ=255)
                                                                                    ttGTCACGCCACTGTGCGGCACGCCGAGATAACTGATATTACGATGCCCCTGGTCATACAGCCGTTGCATCAGGATTTTGATTGCCCCTtcgt          >  2:2531975/1‑93 (MQ=255)
                                                                                     tGTCACGCCACTGTGCGGCACGCCGAGATAACTGATATTACGATGCCCCTGGTCATACAGCCGTTGCATCAGGATTTTGATTGCCCCTtcgtc         <  1:170662/93‑1 (MQ=255)
                                                                                     tGTCACGCCACTGTGCGGCACGCAGAGATAACTGATATTACGATGCCCCTGGTCATACAGCCGTTGCATCAGGATTTTGATTGCCCCTtcgtc         <  2:469367/93‑1 (MQ=255)
                                                                                         acgccacTGTGCGGCACGCCGAGATAACTGATATTACGATGCCCCTGGTCATACAGCCGTTGCATCAGGATTTTGATTGCCCCTTCGTCGTCa     <  2:4341563/93‑1 (MQ=255)
                                                                                            |                                                                                            
TAGCAAGCCCTGGCAGGGCGGCAACGGGATGCAGTTTATGCGCTTTGCAGAACGCCAGGTAGGCTTCGTGACGTCGCTTACCGGTTGTCACGTCACTGTGCGGCACGCCGAGATAACTGATATTACGATGCCCCTGGTCATACAGCCGTTGCATCAGGATTTTGATTGCCCCTTCGTCGTCATAA  >  NC_000913/4464169‑4464353

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TAGCAAGCCCTGGCAGGGCGGCAACGGGATGCAGTTTATGCGCTTTGCAGAACGCCAGGTAGGCTTCGTGACGTCGCTTACCGGTTGTCACGTCACTGTGCGGCACGCCGAGATAACTGATATTACGATGCCCCTGGTCATACAGCCGTTGCATCAGGATTTTGATTGCCCCTTCGTCGTCATAAC  >  NC_000913/4464169‑4464354
                                                                                            |                                                                                             
TAGCAAGCCCTGGCAGGGCGGCAACGGGATGCAGTTTATGCGCTTTGCAGAACGCCAGGTAGGCTTCGTGACGTCGCTTACCGGTTGTCACGTC                                                                                              <  SRR4302537.5459447/94‑1 (MQ=60)
TAGCAAGCCCTGGCAGGGCGGCAACGGGATGCAGTTTATGCGCTTTGCAGAACGCCAGGTAGGCTTCGTGACGTCGCTTACCGGTTGTCACGCC                                                                                              <  SRR4302537.83704/94‑1 (MQ=60)
TAGCAAGCCCTGGCAGGGCGGCAACGGGATGCAGTTTATGCGCTTTGCAGAACGCCAGGTAGGCTTCGTGACGTCGCTTACCGGTTGTCACGCC                                                                                              <  SRR4302537.2129435/94‑1 (MQ=60)
 AGCAAGCCCTGGCAGGGCGGCAACGGGATGCAGTTTATGCGCTTTGCAGAACGCCAGGGAGGCTTCGTGACGTCGCTTACCGGTTGTCACGCCA                                                                                             >  SRR4302537.986913/1‑94 (MQ=60)
   CAAGCCCTGGCAGGGCGGCAACGGGATGCAGTTTATGCGCTTTGCAGAACGCCAGGTAGGCTTCGTGACGTCGCTTACCGGTTGTCACGCCACT                                                                                           <  SRR4302537.3012722/94‑1 (MQ=60)
   CAAGCCCTGGCAGGGCGGCAACGGGATGCAGTTTATGCGCTTTGCAGAACGCCAGGTAGGCTTCGTGACGTCGCTTACCGGTTGGCACGCCACT                                                                                           <  SRR4302537.1873779/94‑1 (MQ=60)
    AAGCCCTGGCAGGGCGGCAACGGGATGCAGTTTATGCGCTTTGCAGAACGCCAGGTAGGCTTCGTGACGTCGCTTACCGGTTGTCACGCCACTG                                                                                          <  SRR4302537.4515115/94‑1 (MQ=60)
    AAGCCCTGGCAGGGCGGCAACGGGATGCAGTTTATGCGCTTTGCAGAACGCCAGGTAGGCTTCGTGACGTCGCTTACCGGTTGTCACGCCACTG                                                                                          >  SRR4302537.3610359/1‑94 (MQ=60)
     AGCCCTGGCAGGGCGGCAACGGGATGCAGTTTATGCGCTTTGCAGAACGCCAGGTAGGCTTCGTGACGTCGCTTACCGGTTGTCACGCCACTGT                                                                                         >  SRR4302537.5774910/1‑94 (MQ=60)
     AGCCCTGGCAGGGCGGCAACGGGATGCAGTTTATGCGCTTTGCAGAACGCCAGGTAGGCTTCGTGACGTCGCTTACCGGTTGTCACGCCACTGT                                                                                         >  SRR4302537.4173602/1‑94 (MQ=60)
      GCCCTGGCAGGGCGGCAACGGGATGCAGTTTATGCGCTTTGCAGAACGCCAGGTAGGCTTCGTGACGTCGCTTACCGGTTGTCACGCCACTGTG                                                                                        <  SRR4302537.2587668/94‑1 (MQ=60)
      GCCCTGGCAGGGCGGCAACGGGATGCAGTTTATGCGCTTTGCAGAACGCCAGGTAGGCTTCGTGACGTCGCTTACCGGTTGTCACGCCACTGTG                                                                                        <  SRR4302537.2753852/94‑1 (MQ=60)
       CCCTGGCAGGGCGGCAACGGGATGCAGTTTATGCGCTTTGCAGAACGCCAGGTAGGCTTCGTGACGTCGCTTACCGGTTGTCACGCCACTGTGC                                                                                       >  SRR4302537.4380050/1‑94 (MQ=60)
        CCTGGCAGGGCGGCAACGGGATGCAGTTTATGCGCTTTGCAGAACGCCAGGTAGGCTTCGTGACGTCGCTTACCGGTTGTCACGCCACTGTGCG                                                                                      <  SRR4302537.2137926/94‑1 (MQ=60)
        CCTGGCAGGGCGGCAACGGGATGCAGTTTATGCGCTTTGCAGAACGCCAGGTAGGCTTCGTGACGTCGCTTACCGGTTGTCACGCCACTGTGCG                                                                                      >  SRR4302537.2012382/1‑94 (MQ=60)
        CCTGGCAGGGCGGCAACGGGATGCAGTTTATGCGCTTTGCAGAACGCCAGGTAGGCTTCGTGACGTCGCTTACCGGTTGTCACGCCACTGTGCG                                                                                      >  SRR4302537.6008361/1‑94 (MQ=60)
        CCTGGCAGGGCGGCAACGGGATGCAGTTTATGCGCTTTGCAGAACGCCAGGTAGGCTTCGTGACGTCGCTTACCGGTTGTCACGCCACTGTGCG                                                                                      <  SRR4302537.78032/94‑1 (MQ=60)
        CCTGGCAGGGCGGCAACGGGATGCAGTTTATGCGCTTTGCAGAACGCCAGGTAGGCTTCGTGACGTCGCTTACCGGTTGTCACGACACTGTGCG                                                                                      >  SRR4302537.5413368/1‑94 (MQ=60)
         CTGGCAGGGCGGCAACGGGATGCAGTTTATGCGCTTTGCAGAACGCCAGGTAGGCTTCGTGACGTCGCTTACCGGTTGTCACGCCACTGTGCGG                                                                                     >  SRR4302537.4415782/1‑94 (MQ=60)
         CTGGCAGGGCGGCAACGGGATGCAGTTTATGCGCTTTGCAGAACGCCAGGTAGGCTTCGTGACGTCGCTTACCGGTTGTCACGCCACTGTGCGG                                                                                     >  SRR4302537.1228152/1‑94 (MQ=60)
         CTGGCAGGGCGGCAACGGGATGCAGTTTATGCGCTTTGCAGAACGCCAGGTAGGCTTCGTGACGTCGCTTACCGGTTGTCACGCCACTGTGCGG                                                                                     >  SRR4302537.2431280/1‑94 (MQ=60)
          TGGCAGGGCGGCAACGGGATGCAGTTTATGCGCTTTGCAGAACGCCAGGTAGGCTTCGTGACGTCGCTTACCGGTTGTCACGCCACTGTGCGGC                                                                                    >  SRR4302537.3086247/1‑94 (MQ=60)
          TGGCAGGGCGGCAACGGGATGCAGTTTATGCGCTTTGCAGAACGCCAGGTAGGCTTCGTGACGTCGCTTACCGGTTGTCACGCCACTGTGCGGC                                                                                    >  SRR4302537.4251695/1‑94 (MQ=60)
          TGGCAGGGCGGCAACGGGATGCAGTTTATGCGCTTTGCAGAACGCCAGGTAGGCTTCGTGACGTCGCTTACCGGTTGTCACGCCACTGTGCGGC                                                                                    >  SRR4302537.1106806/1‑94 (MQ=60)
           GGCAGGGCGGCAACGGGATGCAGTTTATGCGCTTTGCAGAACGCCAGGTAGGCTTCGTGACGTCGCTTACCGGTTGTCACGCCACTGTGCGGCA                                                                                   <  SRR4302537.940047/94‑1 (MQ=60)
             CAGGGCGGCAACGGGATGCAGTTTATGCGCTTTGCAGAACGCCAGGTAGGCTTCGTGACGTCGCTTACCGGTTGTCACGCCACTGTGCGGCACG                                                                                 >  SRR4302537.935710/1‑94 (MQ=60)
              AGGGCGGCAACGGGATGCAGTTTATGCGCTTTGCAGAACGCCAGGTAGGCTTCGTGACGTCGCTTACCGGTTGTCACGCCACTGTGCGGCACGC                                                                                >  SRR4302537.4620174/1‑94 (MQ=60)
              AGGGCGGCAACGGGATGCAGTTTATGCGCTTTGCAGAACGCCAGGTAGGCTTCGTGACGTCGCTTACCGGTTGTCACGCCACTGTGCGGCACGC                                                                                <  SRR4302537.1459595/94‑1 (MQ=60)
                 GCGGCAACGGGAGGCAGTTTATGCGCTTTGCAGAACGCCAGGTAGGCTTCGTGACGTCGCTTACCGGTTGTCACGCCACTGTGCGGCACGCCGA                                                                             <  SRR4302537.1293623/94‑1 (MQ=60)
                      AACGGGATGCAGTTTATGCGCTTTGCAGAACGCCAGGTAGGCTTCGTGACGTCGCTTACCGGTTGTCACGCCACTGTGCGGCACGCCGAGATAA                                                                        <  SRR4302537.3691716/94‑1 (MQ=60)
                      AACGGGATGCAGTTTATGCGCTTTGCAGAACGCCAGGTAGGCTTCGTGACGTCGCTTACCGGTTGTCACGCCACTGTGCGGCACGCCGAGATAA                                                                        <  SRR4302537.3034030/94‑1 (MQ=60)
                      AACGGGATGCAGTTTATGCGCTTTGCAGAACGCCAGGTAGGCTTCGTGACGTCGCTTACCGGTTGTCACGCCACTGTGCGGCACGCCGAGATAA                                                                        >  SRR4302537.2181479/1‑94 (MQ=60)
                       ACGGGATGCAGTTTATGCGCTTTGCAGAACGCCAGGTAGGCTTCGTGACGTCGCTTACCGGTTGTCACGCCACTGTGCGGCACGCCGAGATAAC                                                                       <  SRR4302537.1274510/94‑1 (MQ=60)
                         GGGATGCAGTTTATGCGCTTTGCAGAACGCCAGGTAGGCTTCGTGACGTCGCTTACCGGTTGTCACGCCACTGTGCGGCACGCCGAGATAACTG                                                                     <  SRR4302537.4380856/94‑1 (MQ=60)
                          GGATGCAGTTTATGCGCTTTGCAGAACGCCAGGTAGGCTTCGTGACGTCGCTTACCGGTTGTCACGCCACTGTGCGGCACGCCGAGATAACTGA                                                                    >  SRR4302537.1383890/1‑94 (MQ=60)
                           GATGCAGTTTATGCGCTTTGCAGAACGCCAGGTAGGCTTCGTGTCGTCGCTTACCGGTTGTCACGCCACTGTGCGGCACGCCGAGATAACTGAT                                                                   >  SRR4302537.3486464/1‑94 (MQ=60)
                           GATGCAGTTTATGCGCTTTGCAGAACGCCAGGTAGGCTTCGTGACGTCGCTTACCGGTTGTCACGCCACTGTGCGGCACGCCGAGATAACTGAT                                                                   <  SRR4302537.1226978/94‑1 (MQ=60)
                            ATGCAGTTTATGCGCTTTGCAGAACGCCAGGTAGGCTTCGTGACGTCGCTTACCGGTTGTCACGCCACTGTGCGGCACGCCGAGATAACTGATA                                                                  >  SRR4302537.1405752/1‑94 (MQ=60)
                            ATGCAGTTTATGCGCTTTGCAGAACGCCAGGTAGGCTTCGTGACGTCGCTTACCGGTTGTCACGCCACTGTGCGGCACGCCGAGATAACTGATA                                                                  >  SRR4302537.1937326/1‑94 (MQ=60)
                              GCAGTTTATGCGCTTTGCAGAACGCCAGGTAGGCTTCGTGACGTCGCTTACCGGTTGTCACGCCACTGTGCGGCACGCCGAGATAACTGATATT                                                                <  SRR4302537.2856276/94‑1 (MQ=60)
                                AGTTTATGCGCTTTGCAGAACGCCAGGTAGGCTTCGTGACGTCGCTTACCGGTTGTCACGCCACTGTGCGGCACGCCGAGATAACTGATATTAC                                                              <  SRR4302537.3345763/94‑1 (MQ=60)
                                AGTTTATGCGCTTTGCAGAACGCCAGGTAGGCTTCGTGACGTCGCTTACCGGTTGTCACGCCACTGTGCGGCACGCCGAGATAACTGATATTAC                                                              <  SRR4302537.4776985/94‑1 (MQ=60)
                                AGTTTATGCGCTTTGCAGAACGCCAGGTAGGCTTCGTGACGTCGCTTACCGGTTGTCACGCCACTGTGCGGCACGCCGAGATAACTGATATTAC                                                              <  SRR4302537.3053290/94‑1 (MQ=60)
                                  TTTATGCGCTTTGCAGAACGCCAGGTAGGCTTCGTGACGTCGCTTACCGGTTGTCACGCCACTGTGCGGCACGCCGAGATAACTGATATTACGA                                                            <  SRR4302537.224918/94‑1 (MQ=60)
                                   TTATGCGCTTTGCAGAACGCCAGGTAGGCTTCGTGACGTCGCTTACCGGTTGTCACGCCACTGTGCGGCACGCCGAGATAACTGATATTACGAT                                                           >  SRR4302537.3199164/1‑94 (MQ=60)
                                   TTATGCGCTTTGCAGAACGCCAGGTAGGCTTCGTGACGTCGCTTACCGGTTGTCACGCCACTGTGCGGCACGCCGAGATAACTGATATTACGAT                                                           >  SRR4302537.3376407/1‑94 (MQ=60)
                                   TTATGCGCTTTGCAGAACGCCAGGTAGGCTTCGTGACGTCGCTTACCGGTTGTCACGCCACTGTGCGGCACGCCGAGATAACTGATATTACGAT                                                           <  SRR4302537.1149123/94‑1 (MQ=60)
                                    TATGCGCTTTGCAGAACGCCAGGTAGGCTTCGTGACGTCGCTTACCGGTTGTCACGCCACTGTGCGGCACGCCGAGATAACTGATATTACGATG                                                          <  SRR4302537.5410844/94‑1 (MQ=60)
                                    TATGCGCTTTGCAGAACGCCAGGTAGGCTTCGTGACGTCGCTTACCGGTTGTCACGCCACTGTGCGGCACGCCGAGATAACTGATATTACGATG                                                          >  SRR4302537.2453968/1‑94 (MQ=60)
                                    TATGCGCTTTGCAGAACGACAGGTAGGCTTCGTGACGTCGCTTACCGGTTGTCACGCCACTGGGCGGCACGCCGCGATAACTGATATTACGATG                                                          >  SRR4302537.1074322/1‑94 (MQ=60)
                                     ATGCGCTTTGCAGAACGCCAGGTAGGCTTCGTGACGTCGCTTACCGGTTGTCACGCCACTGTGCGGCACGCCGAGATAACTGATATTACGATGC                                                         >  SRR4302537.712092/1‑94 (MQ=60)
                                       GCGCTTTGCAGAACGCCAGGTAGGCTTCGTGACGTCGCTTACCGGTTGTCACGCCACTGTGCGGCACGCCGAGATAACTGATATTACGATGCCC                                                       <  SRR4302537.3886205/94‑1 (MQ=60)
                                        CGCTTTGCAGAACGCCAGGTAGGCTTCGTGACGTCGCTTACCGGTTGTCACGTCACTGTGCGGCACGCCGAGATAACTGATATTACGATGCCCC                                                      >  SRR4302537.5435309/1‑94 (MQ=60)
                                         GCTTTGCAGAACGCCAGGTAGGCTTCGTGACGTCGCTTACCGGTTGTCACGCCACTGTGCGGCACGCCGAGATAACTGATATTACGATGCCCCT                                                     <  SRR4302537.1414911/94‑1 (MQ=60)
                                         GCTTTGCAGAACGCCAGGTAGGCTTCGTGACGTCGCTTACCGGTTGTCACGCCACTGTGCGGCACGCCGAGATAACTGATATTACGATGCCCCT                                                     >  SRR4302537.4927265/1‑94 (MQ=60)
                                         GCTTTGCAGAACGCCAGGTAGGCTTCGTGACGTCGCTTACCGGTTGTCACGCCACTGTGCGGCACGCCGAGATAACTGATATTACGATGCCCCT                                                     <  SRR4302537.1236426/94‑1 (MQ=60)
                                         GCTTTGCAGAACGCCAGGTAGGCTTCGTGACGTCGCTTACCGGTTGTCACGCCACTGTGCGGCACGCCGAGATAACTGATATTACGATGCCCCT                                                     >  SRR4302537.1731244/1‑94 (MQ=60)
                                         GCTTTGCAGAACGCCAGGTAGGCTTCGTGACGTCGCTTACCGGTTGTCACGCCACTGTGCGGCACGCCGAGATAACTGATATTACGATGCCCCT                                                     >  SRR4302537.2062076/1‑94 (MQ=60)
                                          CTTTGCAGAACGCCAGGTAGGCTTCGTGACGTCGCTTACCGGTTGTCACGTCACTGTGCGGCACGCCGAGATAACTGATATTACGATGCCCCTG                                                    >  SRR4302537.6306605/1‑94 (MQ=60)
                                          CTTTGCAGAACGCCAGGTAGGCTTCGTGACGTCGCTTACCGGTTGTCACGTCACTGTGCGGCACGCCGAGATAACTGATATTACGATGCCCCTG                                                    >  SRR4302537.4722465/1‑94 (MQ=60)
                                           TTTGCAGAACGCCAGGTAGGCTTCGTGACGTCGCTTACCGGTTGTCACGCCACTGTGCGGCACGCCGAGATAACTGATATTACGATGCCCCTGG                                                   <  SRR4302537.538207/94‑1 (MQ=60)
                                              GCAGAACGCCAGGTAGGCTTCGTGACGTCGCTTACCGGTTGTCACGCCACTGTGCGGCACGCCGTGATAACTGATATTACGATGCCCCTGGTCA                                                <  SRR4302537.564008/94‑1 (MQ=60)
                                               CAGAACGCCAGGTAGGCTTCGTGACGTCGCTTACCGGTTGTCACGCCACTGTGCGGCACGCCGAGATAACTGATATTACGATGCCCCTGGTCAT                                               >  SRR4302537.6375607/1‑94 (MQ=60)
                                                 GAACGCCAGGTAGGCTTCGTGACGTCGCTTACCGGTTGTCACGCCACTGTGCGGCACGCCGAGATAACTGATATTACGATGCCCCTGGTCATAC                                             <  SRR4302537.4154877/94‑1 (MQ=60)
                                                  AACGCCAGGTAGGCTTCGTGACGTCGCTTACCGGTTGTCACGCCACTGTGCGGCACGCCGAGATAACTGATATTACGATGCCCCTGGTCATACA                                            <  SRR4302537.2766534/94‑1 (MQ=60)
                                                    CGCCAGGTAGGCTTCGTGACGTCGCTTACCGGTTGTCACGCCACTGTGCGGCACGCCGAGATAACTGATATTACGATCCCCCTGGCCATACAGC                                          >  SRR4302537.4711421/1‑94 (MQ=60)
                                                     GCCAGGTAGGCTTCGTGACGTCGCTTACCGGTTGTCACGCCACTGTGCGGCACGCCGAGATAACTGATATTACGATGCCCCTGGTCATACAGCC                                         <  SRR4302537.3802074/94‑1 (MQ=60)
                                                     GCCAGGTAGGCTTCGTGACGTCGCTTACCGGTTGTCACGCCACTGTGCGGCACGCCGAGATAACTGATATTACGATGCCCCTGGTCATACAGCC                                         >  SRR4302537.6482460/1‑94 (MQ=60)
                                                     GCCAGGTAGGCTTCGTGACGTCGCTTACCGGTTGTCACGCCACTGTGCGGCACGCCGAGATAACTGATATTACGATGCCCCTGGTCATACAGCC                                         <  SRR4302537.3429691/94‑1 (MQ=60)
                                                       CAGGTAGGCTTCGTGACGTCGCTTACCGGTTGTCACGCCACTGTGCGGCACGCCGAGATAACTGATATTACGATGCCCCTGGTCATACAGCCGT                                       >  SRR4302537.2228936/1‑94 (MQ=60)
                                                        AGGTAGGCTTCGTGACGTCGCTTACCGGTTGTCACGCCACTGTGCGGCACGCCGAGATAACTGATATTACGATGCCCCTGGTCATACAGCCGTT                                      >  SRR4302537.2837052/1‑94 (MQ=60)
                                                         GGTAGGCTTCGTGACGTCGCTTACCGGTTGTCACGCCACTGTGCGGCACGCCGAGATAACTGATATTACGATGCCCCTGGTCATACAGCCGTTG                                     >  SRR4302537.6365620/1‑94 (MQ=60)
                                                          GTAGGCTTCGTGACGTCGCTTACCGGTTGTCACGCCACTGTGCGGCACGCCGAGATAACTGATATTACGATGCCCCTGGTCATACAGCCGTTGC                                    >  SRR4302537.4008151/1‑94 (MQ=60)
                                                           TAGGCTTCGTGACGTCGCTTACCGGTTGTCACGCCACTGTGCGGCACGCCGAGATAACTGATATTACGATGCCCCTGGTCATACAGCCGTTGCA                                   >  SRR4302537.6509839/1‑94 (MQ=60)
                                                            AGGCTTCGTGACGTCGCTTACCGGTTGTCACGCCACTGTGCGGCACGCCGAGATAACTGATATTACGATGCCCCTGGTCATACAGCCGTTGCAT                                  <  SRR4302537.3122250/94‑1 (MQ=60)
                                                            AGGCTTCGTGACGTCGCTTACCGGTTGTCACGCCACTGTGCGGCACGCCGAGATAACTGATATTACGATGCCCCTGGTCATACAGCCGTTGCAT                                  >  SRR4302537.1258486/1‑94 (MQ=60)
                                                             GGCTTCGTGACGTCGCTTACCGGTTGTCACGCCACTGTGCGGCACGCCGAGATAACTGATATTACGATGCCCCTGGTCATACAGCCGTTGCATC                                 <  SRR4302537.1980923/94‑1 (MQ=60)
                                                             GGCTTCGTGACGTCGCTTACCGGTTGTCACGCCACTGTGCGGCACGCCGAGATAACTGATATTACGATGCCCCTGGTCATACAGCCGTTGCATC                                 <  SRR4302537.3557135/94‑1 (MQ=60)
                                                               CTTCGTGACGTCGCTTACCGGTTGTCACGCCACTGTGCGGCACGCCGAGATAACTGATATTACGATGCCCCTGGTCATACAGCCGTTGCATCAG                               >  SRR4302537.946416/1‑94 (MQ=60)
                                                               CTTCGTGACGTCGCTTACCGGTTGTCACGCCACTGTGCGGCACGCCGAGATAACTGATATTACGATGCCCCTGGTCATACAGCCGTTGCATCAG                               >  SRR4302537.5438079/1‑94 (MQ=60)
                                                               CTTCGTGACGTCGCTTACCGGTTGTCACGCCACTGTGCGGCACGCCGAGATAACTGATATTACGATGCCCCTGGTCATACAGCCGTTGCATCAG                               <  SRR4302537.5346694/94‑1 (MQ=60)
                                                               CTTCGTGACGTCGCTTACCGGTTGTCACGCCACTGTGCGGCACGCCGAGATAACTGATATTACGATGCCCCTGGTCATACAGCCGTTGCATCAG                               >  SRR4302537.5358435/1‑94 (MQ=60)
                                                               CTTCGTGACGTCGCTTACCGGTTGTCACGCCACTGTGCGGCACGCCGAGATAACTGATATTACGATGCCCCTGGTCATACAGCCGTTGCATCAG                               >  SRR4302537.3057084/1‑94 (MQ=60)
                                                                TTCGTGACGTCGCTTACCGGTTGTCACGCCACTGTGCGGCACGCCGAGATAACTGATATTACGATGCCCCTGGTCATACAGCCGTTGCATCAGG                              >  SRR4302537.351842/1‑94 (MQ=60)
                                                                  CGTGACGTCGCTTACCGGTTGTCACGCCACTGTGCGGCACGCCGAGATAACTGATATTACGATGCCCCTGGTCATACAGCCGTTGCATCAGGAT                            >  SRR4302537.333019/1‑94 (MQ=60)
                                                                   GTGACGTCGCTTACCGGTTGTCACGCCACTGTGCGGCACGCCGAGATAACTGATATTACGATGCCCCTGGTCATACAGCCGTTGCATCAGGATT                           <  SRR4302537.2269258/94‑1 (MQ=60)
                                                                   GTGACGTCGCTTACCGGTTGTCACGCCACTGTGCGGCACGCCGAGATAACTGATATTACGATGCCCCTGGTCATACAGCCGTTGCATCAGGATT                           >  SRR4302537.5475106/1‑94 (MQ=60)
                                                                   GTGACGTCGCTTACCGGTTGTCACGCCACTGTGCGGCACGCCGAGATAACTGATATTACGATGCCCCTGGTCATACAGCCGTTGCATCAGGATT                           <  SRR4302537.4726661/94‑1 (MQ=60)
                                                                   GTGACGTCGCTTACCGGTTGTCACGCCACTGTGCGGCACGCCGAGATAACTGATATTACGATGCCCCTGGTCATACAGCCGTTGCATCAGGATT                           >  SRR4302537.2417129/1‑94 (MQ=60)
                                                                     GACGTCGCTTACCGGTTGTCACGCCACTGTGCGGCACGCCGAGATAACTGATATTACGATGCCCCTGGTCATACAGCCGTTGCATCAGGATTTT                         >  SRR4302537.5273829/1‑94 (MQ=60)
                                                                         TCGCTTACCGGTTGTCACGTCACTGTGCGGCACGCCGAGATAACTGATATTACGATGCCCCTGGTCATACAGCCGTTGCATCAGGATTTTGATT                     <  SRR4302537.3442450/94‑1 (MQ=60)
                                                                         TCGCTTACCGGTTGTCACGCCACTGTGCGGCACGCCGAGATAACTGATATTACGATGCCCCTGGTCATACAGCCGTTGCATCAGGATTTTGATT                     <  SRR4302537.4606669/94‑1 (MQ=60)
                                                                          CGCTTACCGGTTGTCACGCCACTGTGCGGCACGCCGAGATAACTGATATTACGATGCCCCTGGTCATACAGCCGTTGCATCAGGATTTTGATTG                    <  SRR4302537.2372766/94‑1 (MQ=60)
                                                                          CGCTTACCGGTTGTCACGCCACTGTGCGGCACGCCGAGATAACTGATATTACGATGCCCCTGGTCATACAGCCGTTGCATCAGGATTTTGATTG                    >  SRR4302537.340994/1‑94 (MQ=60)
                                                                             TTACCGGTTGTCACGCCACTGTGCGGCACGCCGAGATAACTGATATTACGATGCCCCTGGTCATACAGCCGTTGCATCAGGATTTTGATTGCCC                 <  SRR4302537.289800/94‑1 (MQ=60)
                                                                             TTACCGGTTGTCACGCCACTGTGCGGCACGCCGAGATAACTGATATTACGATGCCCCTGGTCATACAGCCGTTGCATCAGGATTTTGATTGCCC                 <  SRR4302537.2926089/94‑1 (MQ=60)
                                                                               ACCGGTTGTCACGCCACTGTGCGGCACGCCGAGATAACTGATATTACGATGCCCCTGGTCATACAGCCGTTGCATCAGGATTTTGATTGCCCCT               <  SRR4302537.4711189/94‑1 (MQ=60)
                                                                               ACCGGTTGTCACGCCACTGTGCGGCACGCCGAGATAACTGATATTACGATGCCCCTGGTCATACAGCCGTTGCATCAGGATTTTGATTGCCCCT               >  SRR4302537.350221/1‑94 (MQ=60)
                                                                                 CGGTTGTCACGCCACTGTGCGGCACGCCGAGATAACTGATATTACGATGCCCCTGGTCATACAGCCGTTGCATCAGGATTTTGATTGCCCCTTC             >  SRR4302537.1582554/1‑94 (MQ=60)
                                                                                  GTTTGTCACGCCACTGTGCGGCACGCCGAGATAACTGATATTACGATGCCCCTGGTCATACAGCCGTTGCATCAGGATTTTGATTGCCCCTTCG            <  SRR4302537.4952417/94‑1 (MQ=60)
                                                                                  GGTTGTCACGCCACTGTGCGGCACGCCGAGATAACTGATATTACGATGCCCCTGGTCATACAGCCGTTGCATCAGGATTTTGATTGCCCCTTCG            <  SRR4302537.749027/94‑1 (MQ=60)
                                                                                  GGTTGTCACGCCACTGTGCGGCACGCCGAGATAACTGATATTACGATGCCCCTGGTCATACAGCCGTTGCATCAGGATTTTGATTGCCCCTTCG            >  SRR4302537.3378189/1‑94 (MQ=60)
                                                                                  GGTTGTCACGCCACTGTGCGGCACGCCGAGATAACTGATATTACGATGCCCCTGGTCATACAGCCGTTGCATCAGGATTTTGATTGCCCCTTCG            >  SRR4302537.952986/1‑94 (MQ=60)
                                                                                   GTTGTCACGCCACTGTGCGGCACGCCGAGATAACTGATATTACGATGCCCCTGGTCATACAGCCGTTGCATCAGGATTTTGATTGCCCCTTCGT           >  SRR4302537.1220508/1‑94 (MQ=60)
                                                                                    TTGTCACGCCACTGTGCGGCACGCCGAGATAACTGATATTACGATGCCCCTGGTCATACAGCCGTTGCATCAGGATTTTGATTGCCCCTTCGTC          <  SRR4302537.379873/94‑1 (MQ=60)
                                                                                     TGTCACGCCACTGTGCGGCACGCCGAGATAACTGATATTACGATGCCCCTGGTCATACAGCCGTTGCATCAGGATTTTGATTGCCCCTTCGTCG         <  SRR4302537.171877/94‑1 (MQ=60)
                                                                                           GCCACTGTGCGGCACGCCGAGATAACTGATATTACGATGCCCCTGGTCATACAGCCGTTGCATCAGGATTTTGATTGCCCCTTCGTCGTCATAA   <  SRR4302537.2767924/94‑1 (MQ=60)
                                                                                            CCACTGTGCGGCACGCCGAGATAACTGATATTACGATGCCCCTGGTCATACAGCCGTTGCATCAGGATTTTGATTGCCCCTTCGTCGTCATAAC  <  SRR4302537.716723/94‑1 (MQ=60)
                                                                                            CCACTGTGCGGCACGCCGAGATAACTGATATTACGATGCCCCTGGTCATACAGCCGTTGCATCAGGATTTTGATTGCCCCTTCGTCGTCATAAC  <  SRR4302537.461510/94‑1 (MQ=60)
                                                                                            |                                                                                             
TAGCAAGCCCTGGCAGGGCGGCAACGGGATGCAGTTTATGCGCTTTGCAGAACGCCAGGTAGGCTTCGTGACGTCGCTTACCGGTTGTCACGTCACTGTGCGGCACGCCGAGATAACTGATATTACGATGCCCCTGGTCATACAGCCGTTGCATCAGGATTTTGATTGCCCCTTCGTCGTCATAAC  >  NC_000913/4464169‑4464354

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑