Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 882 254.4 12957588 98.0% 12698436 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,474,826 +A intergenic (+1/+44) yjgL → / ← argI hypothetical protein/ornithine carbamoyltransferase 1

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,474,8231.A100.0% 755.4 / NA 210*617K (TAA→AAA) yjgLhypothetical protein
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base A (115/95);  total (115/95)

Only 100 of 228 total aligned reads displayed.

TTAATGAACAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCAT‑AAATAATGTGATGCCGGGATGGTTTCCGCATCTTATAGCGATCAGATTATTTACTGAGCGTCGCGACCATCACCGCTTTGATAGTAT  >  NC_000913/4474731‑4474910
                                                                                             |                                                                                       
ttAATGAACAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCat                                                                                          >  1:3691954/1‑93 (MQ=255)
ttAATGAACAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCat                                                                                          >  1:1342606/1‑93 (MQ=255)
ttAATGAACAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCat                                                                                          >  2:59381/1‑93 (MQ=255)
ttAATGAACAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATAACTGCat                                                                                          >  1:2479712/1‑93 (MQ=255)
    tGAACAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCat‑aaaa                                                                                     >  2:5616141/1‑92 (MQ=255)
    tGAACAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCat‑aaaa                                                                                     <  2:2869433/93‑2 (MQ=255)
    tGAACAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCat‑aaaa                                                                                     >  2:2413335/1‑92 (MQ=255)
    tGAACAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCat‑aaaa                                                                                     >  2:6281286/1‑92 (MQ=255)
     gAACAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCat‑aaaat                                                                                    >  1:1058694/1‑91 (MQ=255)
     gAACAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCat‑aaaat                                                                                    >  1:2878249/1‑91 (MQ=255)
      aaCAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCat‑aaaata                                                                                   >  2:3671772/1‑90 (MQ=255)
         aGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAAtg                                                                                 <  2:6314973/93‑1 (MQ=255)
         aGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAAtg                                                                                 >  1:1482430/1‑93 (MQ=255)
         aGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAAtg                                                                                 >  2:2898503/1‑93 (MQ=255)
         aGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAAtg                                                                                 >  1:2929945/1‑93 (MQ=255)
          gCGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAAtgt                                                                                >  2:5116540/1‑93 (MQ=255)
           cGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAAtgtg                                                                               <  1:1454483/93‑1 (MQ=255)
            gTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGa                                                                              <  2:665471/93‑1 (MQ=255)
            gTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGa                                                                              <  1:3000856/93‑1 (MQ=255)
             tttAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGAt                                                                             >  1:1529964/1‑93 (MQ=255)
             tttAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGAt                                                                             >  1:934472/1‑93 (MQ=255)
             tttAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGAt                                                                             >  1:3358074/1‑93 (MQ=255)
              ttAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATg                                                                            >  2:12393/1‑93 (MQ=255)
              ttAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATg                                                                            <  1:3206376/93‑1 (MQ=255)
                aaataaaCTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGcc                                                                          >  2:3368160/1‑93 (MQ=255)
                  ataaaCTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCgg                                                                        >  1:3730047/1‑93 (MQ=255)
                   taaaCTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCggg                                                                       >  1:3669862/1‑93 (MQ=255)
                    aaaCTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGa                                                                      >  2:4378472/1‑93 (MQ=255)
                       cTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATgg                                                                   >  2:839634/1‑93 (MQ=255)
                        tAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGt                                                                  >  2:5558090/1‑93 (MQ=255)
                          aaTCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGttt                                                                >  1:3991663/1‑93 (MQ=255)
                          aaTCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGttt                                                                <  1:4799243/93‑1 (MQ=255)
                          aaTCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGttt                                                                >  2:3733153/1‑93 (MQ=255)
                          aaTCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGttt                                                                >  2:3314179/1‑93 (MQ=255)
                             ccACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgta                                                             <  2:3260445‑M1/93‑4 (MQ=255)
                             ccACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgta                                                             <  2:493496‑M1/93‑4 (MQ=255)
                              cACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtat                                                            >  2:4584880‑M1/1‑89 (MQ=255)
                              cACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtat                                                            >  1:2646666‑M1/1‑89 (MQ=255)
                                ccGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtattt                                                          >  2:1225106‑M1/1‑87 (MQ=255)
                                  ggAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcc                                                        <  1:5275681‑M1/93‑9 (MQ=255)
                                   gAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttccc                                                       <  2:1800765‑M1/93‑10 (MQ=255)
                                   gAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttccc                                                       <  1:6154571‑M1/93‑10 (MQ=255)
                                   gAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttccc                                                       >  1:5611268‑M1/1‑84 (MQ=255)
                                    aaaaTTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccg                                                      >  2:5640542‑M1/1‑83 (MQ=255)
                                      aaTTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggc                                                    >  1:4868150‑M1/1‑81 (MQ=255)
                                       aTTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggca                                                   <  2:2205477‑M1/93‑14 (MQ=255)
                                        tttACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcat                                                  <  1:277037‑M1/93‑15 (MQ=255)
                                        tttACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcat                                                  >  1:4856030‑M1/1‑79 (MQ=255)
                                             gTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatcttta                                             >  2:1297076‑M1/1‑74 (MQ=255)
                                              tataGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttat                                            >  2:6122902‑M1/1‑73 (MQ=255)
                                              tataGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttat                                            >  1:6204594‑M1/1‑73 (MQ=255)
                                              tataGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttat                                            >  2:2627200‑M1/1‑73 (MQ=255)
                                              tataGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttat                                            <  2:1705557‑M1/93‑21 (MQ=255)
                                              tataGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttat                                            >  2:1454842‑M1/1‑73 (MQ=255)
                                              tataGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttat                                            <  1:2289551‑M1/93‑21 (MQ=255)
                                               ataGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttata                                           <  1:1146192‑M1/93‑22 (MQ=255)
                                                taGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatag                                          <  2:4454420‑M1/93‑23 (MQ=255)
                                                taGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatag                                          >  1:1804389‑M1/1‑71 (MQ=255)
                                                taGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatag                                          <  2:355753‑M1/93‑23 (MQ=255)
                                                  gCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcg                                        >  2:3208110‑M1/1‑69 (MQ=255)
                                                   cAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcga                                       <  2:1624753‑M1/93‑26 (MQ=255)
                                                   cAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcga                                       <  1:4883096‑M1/93‑26 (MQ=255)
                                                    aaTAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgat                                      >  2:6449547‑M1/1‑67 (MQ=255)
                                                    aaTAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgat                                      >  2:4341753‑M1/1‑67 (MQ=255)
                                                     aTAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgata                                     <  2:309667‑M1/93‑28 (MQ=255)
                                                        gAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgatagca                                  >  1:5158546‑M1/1‑63 (MQ=255)
                                                         aaCACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgatagcaa                                 >  1:4700836‑M1/1‑62 (MQ=255)
                                                          acacTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgatagcaat                                <  1:687049‑M1/93‑33 (MQ=255)
                                                           cacTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgatagcaatt                               >  1:4507268‑M1/1‑60 (MQ=255)
                                                             cTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgatagcaattat                             <  1:1180264‑M1/93‑36 (MQ=255)
                                                              tttGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgatagcaattatt                            <  2:2314506‑M1/93‑37 (MQ=255)
                                                               ttGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgatagcaattattt                           <  1:3118770‑M1/93‑38 (MQ=255)
                                                               ttGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgatagcaattattt                           <  2:4211720‑M1/93‑38 (MQ=255)
                                                                  ggTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgatagcaattatttact                        <  1:5236722‑M1/93‑41 (MQ=255)
                                                                  ggTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgatagcaattatttact                        <  1:244372‑M1/93‑41 (MQ=255)
                                                                   gTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgatagcaattatttactg                       <  2:4693051‑M1/93‑42 (MQ=255)
                                                                   gTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgatagcaattatttactg                       >  1:4263424‑M1/1‑52 (MQ=255)
                                                                    tGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgatagcaattatttactca                      <  2:4072448‑M1/93‑43 (MQ=255)
                                                                       aataatagACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgatagcaattatttactgagcg                   <  1:4056861‑M1/90‑46 (MQ=255)
                                                                       aagaaTAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgatagcaattatttactgagcg                   >  1:3276922‑M1/1‑48 (MQ=255)
                                                                        agaaTAGACCTATCACTGCATAAAATAGTGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgatagcaattatttactgagcgt                  >  1:3048244‑M1/1‑47 (MQ=255)
                                                                        agaaTAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgatagcaattatttactgagcgt                  >  2:4881670‑M1/1‑47 (MQ=255)
                                                                         gaaTAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgatagcaattatttactgagcgtc                 <  2:524770‑M1/93‑48 (MQ=255)
                                                                         gaaTAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgatagcaattatttactgagcgtc                 >  2:3875952‑M1/1‑46 (MQ=255)
                                                                         gaaTAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgatagcaattatttactgagcgtc                 <  1:2957927‑M1/93‑48 (MQ=255)
                                                                         gaaTAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgatagcaattatttactgagcgtc                 >  1:5054685‑M1/1‑46 (MQ=255)
                                                                          aaTAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgatagcaattatttactgagcgtcg                >  2:2819341‑M1/1‑45 (MQ=255)
                                                                           aTAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgatagcaattatttactgagcgtcgc               <  2:4024854‑M1/93‑50 (MQ=255)
                                                                            tAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgatagcaattatttactgagcgtcgcg              >  1:4311585‑M1/1‑43 (MQ=255)
                                                                            tAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgatagcaattatttactgagcgtcgcg              >  1:5502666‑M1/1‑43 (MQ=255)
                                                                             aGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgatagcaattatttactgagcgtcgcga             >  1:5310964‑M1/1‑42 (MQ=255)
                                                                             aGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgatagcaattatttactgagcgtcgcga             >  1:1634795‑M1/1‑42 (MQ=255)
                                                                              gACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtctttcccggcatctttatagcgatagcaattatttactgagcgtcgcgac            >  1:2074428‑M1/1‑41 (MQ=255)
                                                                              gACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgatagcaattatttactgagcgtcgcgac            <  1:3840229‑M1/93‑53 (MQ=255)
                                                                                  tATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgatagcaattatttcctgagcgtcgcgaccatc        <  1:1560265‑M1/93‑57 (MQ=255)
                                                                                    tCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgatagcaattatttactgagcgtcgcgaccatcac      >  1:4616223‑M1/1‑35 (MQ=255)
                                                                                     cACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgatagcaattatttactgagcgtcgcgaccatcacc     <  2:2048276‑M1/93‑60 (MQ=255)
                                                                                       cTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgatagcaattatttactgagcgtcgcgaccatcaccgc   <  2:1052167‑M1/93‑62 (MQ=255)
                                                                                       cTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgatagcaattatttactgagcgtcgcgaccatcaccgc   >  1:924289‑M1/1‑32 (MQ=255)
                                                                                        tGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgatagcaattatttactgagcgtcgcgaccatcaccgct  >  1:6313209‑M1/1‑31 (MQ=255)
                                                                                             |                                                                                       
TTAATGAACAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCAT‑AAATAATGTGATGCCGGGATGGTTTCCGCATCTTATAGCGATCAGATTATTTACTGAGCGTCGCGACCATCACCGCTTTGATAGTAT  >  NC_000913/4474731‑4474910

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AATGAACAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCAT‑AAATAATGTGATGCCGGGATGGTTT‑‑‑‑‑‑‑‑CCGCATC‑TTATAGCGAT‑‑CAGATTATTTACTGAGCGTCGCGACCATCACCGCTTTGATAGTA  >  NC_000913/4474733‑4474909
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AATGAACAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCAT‑AAA                                                                                                >  SRR4302537.771556/1‑94 (MQ=60)
 ATGAACAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCAT‑AAAA                                                                                               <  SRR4302537.1623485/94‑1 (MQ=60)
  TGAACAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCAT‑AAAat                                                                                              >  SRR4302537.1065993/1‑92 (MQ=60)
  TGAACAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCAT‑AAAat                                                                                              >  SRR4302537.2898442/1‑92 (MQ=60)
   GAACAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGTAAAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCAT‑AAAata                                                                                             <  SRR4302537.2005603/94‑4 (MQ=60)
    AACAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAA                                                                                             >  SRR4302537.4901401/1‑94 (MQ=60)
     ACAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAAT                                                                                            <  SRR4302537.5821158/94‑1 (MQ=60)
     ACAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAAT                                                                                            >  SRR4302537.2198697/1‑94 (MQ=60)
     ACAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAAT                                                                                            >  SRR4302537.2400948/1‑94 (MQ=60)
      CAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATG                                                                                           >  SRR4302537.490987/1‑94 (MQ=60)
      CAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATG                                                                                           >  SRR4302537.1492741/1‑94 (MQ=60)
      CAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATG                                                                                           >  SRR4302537.2950519/1‑94 (MQ=60)
       AGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGT                                                                                          >  SRR4302537.1136820/1‑94 (MQ=60)
       AGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGT                                                                                          >  SRR4302537.2175785/1‑94 (MQ=60)
         CGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGA                                                                                        <  SRR4302537.1464618/94‑1 (MQ=60)
          GTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTTGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGAT                                                                                       >  SRR4302537.6522226/1‑94 (MQ=60)
          GTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGAT                                                                                       <  SRR4302537.3021768/94‑1 (MQ=60)
          GTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGAT                                                                                       >  SRR4302537.3381861/1‑94 (MQ=60)
          GTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGAT                                                                                       >  SRR4302537.940910/1‑94 (MQ=60)
          GTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGAT                                                                                       >  SRR4302537.1540655/1‑94 (MQ=60)
           TTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATG                                                                                      >  SRR4302537.4340801/1‑94 (MQ=60)
            TTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGC                                                                                     >  SRR4302537.3813448/1‑94 (MQ=60)
            TTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGC                                                                                     <  SRR4302537.3228919/94‑1 (MQ=60)
              AAATAAACTAAATCCACCGGAAAATTTACGTATAGGAATAGAACACTTTGGGGGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCG                                                                                   >  SRR4302537.1562928/1‑94 (MQ=60)
              AAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCG                                                                                   >  SRR4302537.295142/1‑94 (MQ=60)
               AATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGG                                                                                  >  SRR4302537.3756249/1‑94 (MQ=60)
                ATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGG                                                                                 >  SRR4302537.3695682/1‑94 (MQ=60)
                 TAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGA                                                                                >  SRR4302537.1445410/1‑94 (MQ=60)
                  AAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGAT                                                                               >  SRR4302537.3882910/1‑94 (MQ=60)
                  AAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGAT                                                                               >  SRR4302537.2690669/1‑94 (MQ=60)
                  AAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGAT                                                                               >  SRR4302537.147683/1‑94 (MQ=60)
                       AAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTT                                                                          <  SRR4302537.5591104/94‑1 (MQ=60)
                       AAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTT                                                                          >  SRR4302537.329248/1‑94 (MQ=60)
                       AAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTT                                                                          >  SRR4302537.4019614/1‑94 (MQ=60)
                       AAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTT                                                                          <  SRR4302537.4601538/94‑1 (MQ=60)
                        AATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTT‑‑‑‑‑‑‑‑G                                                                 >  SRR4302537.3901645/1‑94 (MQ=60)
                        AATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTT‑‑‑‑‑‑‑‑G                                                                 <  SRR4302537.4832356/94‑1 (MQ=60)
                           CCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtat                                                                      >  SRR4302537.5257751/1‑90 (MQ=60)
                           CCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtat                                                                      >  SRR4302537.2665176/1‑90 (MQ=60)
                            CACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatt                                                                     <  SRR4302537.2633443/94‑6 (MQ=60)
                            CACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatt                                                                     >  SRR4302537.2715291/1‑89 (MQ=60)
                                GGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttccc                                                                 >  SRR4302537.936272/1‑85 (MQ=60)
                                GGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttccc                                                                 >  SRR4302537.5649632/1‑85 (MQ=60)
                                GGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttccc                                                                 <  SRR4302537.5311985/94‑10 (MQ=60)
                                 GAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccg                                                                <  SRR4302537.334184/94‑11 (MQ=60)
                                 GAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccg                                                                <  SRR4302537.6196913/94‑11 (MQ=60)
                                  AAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccgg                                                               >  SRR4302537.6324023/1‑83 (MQ=60)
                                  AAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccgg                                                               >  SRR4302537.2840729/1‑83 (MQ=60)
                                   AAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggc                                                              >  SRR4302537.4901742/1‑82 (MQ=60)
                                     ATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcat                                                            >  SRR4302537.4992989/1‑80 (MQ=60)
                                     ATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcat                                                            >  SRR4302537.373131/1‑80 (MQ=60)
                                     ATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcat                                                            <  SRR4302537.4772763/94‑15 (MQ=60)
                                     ATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcat                                                            >  SRR4302537.4889535/1‑80 (MQ=60)
                                      TTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatc                                                           >  SRR4302537.5296700/1‑79 (MQ=60)
                                      TTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatc                                                           >  SRR4302537.499338/1‑79 (MQ=60)
                                      TTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatc                                                           <  SRR4302537.278975/94‑16 (MQ=60)
                                      TTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatc                                                           <  SRR4302537.1698824/94‑16 (MQ=60)
                                          CGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatcttta                                                       >  SRR4302537.2274416/1‑75 (MQ=60)
                                           GTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttat                                                      >  SRR4302537.4195336/1‑74 (MQ=60)
                                           GTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttat                                                      >  SRR4302537.6247355/1‑74 (MQ=60)
                                            TATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttata                                                     <  SRR4302537.2305717/94‑22 (MQ=60)
                                            TATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttata                                                     >  SRR4302537.2968721/1‑73 (MQ=60)
                                             ATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatag                                                    <  SRR4302537.1154164/94‑23 (MQ=60)
                                             ATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatag                                                    >  SRR4302537.1816982/1‑72 (MQ=60)
                                               AGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcg                                                  <  SRR4302537.1409928/94‑25 (MQ=60)
                                               AGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcg                                                  <  SRR4302537.4423520/94‑25 (MQ=60)
                                                GCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcga                                                 >  SRR4302537.3204513/1‑69 (MQ=60)
                                                 CAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgat                                                <  SRR4302537.117783/94‑27 (MQ=60)
                                                 CAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgat                                                <  SRR4302537.1593508/94‑27 (MQ=60)
                                                 CAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgat                                                <  SRR4302537.4916823/94‑27 (MQ=60)
                                                     AGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgatagca                                            >  SRR4302537.5194093/1‑64 (MQ=60)
                                                     AGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgatagca                                            >  SRR4302537.2344945/1‑64 (MQ=60)
                                                      GAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgatagcaa                                           >  SRR4302537.4733354/1‑63 (MQ=60)
                                                       AACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgatagcaat                                          <  SRR4302537.3658940/94‑33 (MQ=60)
                                                       AACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgatagcaat                                          <  SRR4302537.1514398/94‑33 (MQ=60)
                                                        ACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgatagcaatt                                         >  SRR4302537.4538435/1‑61 (MQ=60)
                                                        ACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgatagcaatt                                         <  SRR4302537.691759/94‑34 (MQ=60)
                                                          ACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgatagcaattat                                       >  SRR4302537.2927503/1‑59 (MQ=60)
                                                          ACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgatagcaattat                                       <  SRR4302537.368184/94‑36 (MQ=60)
                                                           CTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgatagcaattatt                                      >  SRR4302537.6401024/1‑58 (MQ=60)
                                                           CTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgatagcaattatt                                      <  SRR4302537.1188477/94‑37 (MQ=60)
                                                            TTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgatagcaattattt                                     <  SRR4302537.5158113/94‑38 (MQ=60)
                                                            TTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgatagcaattattt                                     <  SRR4302537.5287698/94‑38 (MQ=60)
                                                             TTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgatagcaattattta                                    <  SRR4302537.3140564/94‑39 (MQ=60)
                                                                GGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgatagcaattatttactg                                 <  SRR4302537.246103/94‑42 (MQ=60)
                                                                GGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgatagcaattatttactg                                 <  SRR4302537.5272750/94‑42 (MQ=60)
                                                                GGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgatagcaattatttactg                                 <  SRR4302537.2988032/94‑42 (MQ=60)
                                                                GGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgatagcaattatttactg                                 >  SRR4302537.4293048/1‑53 (MQ=60)
                                                                  TGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgatagcaattatttactgag                               >  SRR4302537.5531225/1‑51 (MQ=60)
                                                                  TGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgatagcaattatttactgag                               <  SRR4302537.3075369/94‑44 (MQ=60)
                                                                   GGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgatagcaattatttactgagc                              <  SRR4302537.4441572/94‑45 (MQ=60)
                                                                   GGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgatagcaattatttactgagc                              <  SRR4302537.4687780/94‑45 (MQ=60)
                                                                   GGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgatagcaattatttactgagc                              <  SRR4302537.1244007/94‑45 (MQ=60)
                                                                   GGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgatagcaattatttactgagc                              >  SRR4302537.6156831/1‑50 (MQ=60)
                                                                    GAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgatagcaattatttactgagcg                             <  SRR4302537.3697607/94‑46 (MQ=60)
                                                                    GAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgatagcaattatttactgagcg                             >  SRR4302537.3300048/1‑49 (MQ=60)
                                                                     AATAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgatagcaattatttactgagcgt                            <  SRR4302537.4085210/94‑47 (MQ=60)
                                                                     AAGAATAGACCTATCACTGCATAAAATAGTGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgatagcaattatttactgagcgt                            >  SRR4302537.3069483/1‑48 (MQ=60)
                                                                      AGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgatagcaattatttactgagcgtc                           >  SRR4302537.5089483/1‑47 (MQ=60)
                                                                       GAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTGTATTTCCCGGCATCTTTATAGCGATAGCA‑ATTATTTACTGAGCGTCG                         <  SRR4302537.2978680/94‑1 (MQ=30)
                                                                       GAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTGTATTTCCCGGCATCTTTATAGCGATAGCA‑ATTATTTACTGAGCGTCG                         >  SRR4302537.621295/1‑94 (MQ=30)
                                                                       GAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTGTATTTCCCGGCATCTTTATAGCGATAGCA‑ATTATTTACTGAGCGTCG                         >  SRR4302537.247759/1‑94 (MQ=30)
                                                                       GAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTGTATTTCCCGGCATCTTTATAGCGATAGCA‑ATTATTTACTGAGCGTCG                         <  SRR4302537.4759440/94‑1 (MQ=30)
                                                                         ATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTGTATTTCCCGGCATCTTTATAGCGATAGCA‑ATTATTTACTGAGCGTCGCG                       >  SRR4302537.5540239/1‑94 (MQ=26)
                                                                         ATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTGTATTTCCCGGCATCTTTATAGCGATAGCA‑ATTATTTACTGAGCGTCGCG                       >  SRR4302537.4341550/1‑94 (MQ=26)
                                                                         ATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTGTATTTCCCGGCATCTTTATAGCGATAGCA‑ATTATTTACTGAGCGTCGCG                       <  SRR4302537.4029834/94‑1 (MQ=26)
                                                                          TAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTGTATTTCCCGGCATCTTTATAGCGATAGCA‑ATTATTTACTGAGCGTCGCGA                      >  SRR4302537.5347447/1‑94 (MQ=25)
                                                                          TAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTGTATTTCCCGGCATCTTTATAGCGATAGCA‑ATTATTTACTGAGCGTCGCGA                      >  SRR4302537.1646246/1‑94 (MQ=25)
                                                                           AGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTGTCTTTCCCGGCATCTTTATAGCGATAGCA‑ATTATTTACTGAGCGTCGCGAC                     >  SRR4302537.2089035/1‑94 (MQ=23)
                                                                            GACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTGTATTTCCCGGCATCTTTATAGCGATAGCA‑ATTATTTACTGAGCGTCGCGACC                    <  SRR4302537.3867132/94‑1 (MQ=23)
                                                                               CTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTGTATTTCCCGGCATCTTTATAGCGATAGCA‑ATTATTTACTGAGCGTCGCGACCATC                 >  SRR4302537.487949/1‑94 (MQ=23)
                                                                                 ATCACTGCATAAAATAATGTGATGCCGGGATGGTTTGTATTTCCCGGCATCTTTATAGCGATAGCA‑ATTATTTACTGAGCGTCGCGACCATCAC               >  SRR4302537.4648192/1‑94 (MQ=23)
                                                                                  TCACTGCATAAAATAATGTGATGCCGGGATGGTTTGTATTTCCCGGCATCTTTATAGCGATAGCA‑ATTATTTACTGAGCGTCGCGACCATCACC              <  SRR4302537.3027262/94‑1 (MQ=23)
                                                                                   CACTGCATAAAATAATGTGATGCCGGGATGGTTTGTATTTCCCGGCATCTTTATAGCGATAGCA‑ATTATTTACTGAGCGTCGCGACCATCACCG             <  SRR4302537.3228022/94‑1 (MQ=23)
                                                                                    ACTGCATAAAATAATGTGATGCCGGGATGGTTTGTATTTCCCGGCATCTTTATAGCGATAGCA‑ATTATTTACTGAGCGTCGCGACCATCACCGC            >  SRR4302537.930667/1‑94 (MQ=23)
                                                                                     CTGCATAAAATAATGTGATGCCGGGATGGTTTGTATTTCCCGGCATCTTTATAGCGATAGCA‑ATTATTTACTGAGCGTCGCGACCATCACCGCT           >  SRR4302537.6356421/1‑94 (MQ=25)
                                                                                     CTGCATAAAATAATGTGATGCCGGGATGGTTTGTATTTCCCGGCATCTTTATAGCGATAGCA‑ATTATTTACTGAGCGTCGCGACCATCACCGCT           <  SRR4302537.5318088/94‑1 (MQ=25)
                                                                                       GCATAAAATAATGTGATGCCGGGATGGTTTGTATTTCCCGGCATCTTTATAGCGATAGCA‑ATTATTTACTGAGCGTCGCGACCATCACCGCTTT         <  SRR4302537.1713292/94‑1 (MQ=28)
                                                                                        CATAAAATAATGTGATGCCGGGATGGTTTGTATTTCCCGGCATCTTTATAGCGATAGCA‑ATTATTTACTGAGCGTCGCGACCATCACCGCTTTG        <  SRR4302537.4623827/94‑1 (MQ=30)
                                                                                         ataAAATAATGTGATGCCGGGATGGTTTGTATTTCCCGGCATCTTTATAGCGATAGCA‑ATTATTTACTGAGCGTCGCGACCATCACCGCTTTGA       >  SRR4302537.6180365/4‑94 (MQ=33)
                                                                                         ataAAATAATGTGATGCCGGGATGGTTTGTATTTCCCGGCATCTTTATAGCGATAGCA‑ATTATTTACTGAGCGTCGCGACCATCACCGCTTTGA       <  SRR4302537.531612/91‑1 (MQ=33)
                                                                                          taAAATAATGTGATGCCGGGATGGTTTGTATTTCCCGGCATCTTTATAGCGATAGCA‑ATTATTTACTGAGCGTCGCGACCATCACCGCTTTGAT      <  SRR4302537.4668219/92‑1 (MQ=34)
                                                                                          A‑AAATAATGTGATGCCGGGATGGTTTGTATTTCCCGGCATCTTTATAGCGATAGCA‑ATTATTTACTGAGCGTCGCGACCATCACCGCTTTGATA     >  SRR4302537.6468030/1‑94 (MQ=36)
                                                                                          A‑AAATAATGTGATGCCGGGATGGTTTGTATTTCCCGGCATCTTTATAGCGATAGCA‑ATTATTTACTGAGCGTCGCGACCATCACCGCTTTGATA     >  SRR4302537.1322193/1‑94 (MQ=36)
                                                                                            AAATAATGTGATGCCGGGATGGTTTGTATTTCCCGGCATCTTTATAGCGATAGCA‑ATTATTTACTGAGCGTCGCGACCATCACCGCTTTGATAG    >  SRR4302537.4567327/1‑94 (MQ=38)
                                                                                            AAATAATGTGATGCCGGGATGGTTTGTATTTCCCGGCATCTTTATAGCGATAGCA‑ATTATTTACTGAGCGTCGCGACCATCACCGCTTTGATAG    <  SRR4302537.2590566/94‑1 (MQ=38)
                                                                                            AAATAATGTGATGCCGGGATGGTTTGTATTTCCCGGCATCTTTATAGCGATAGCA‑ATTATTTACTGAGCGTCGCGACCATCACCGCTTTGATAG    <  SRR4302537.2645603/94‑1 (MQ=38)
                                                                                             AATAATGTGATGCCGGGATGGTTTGTATTTCCCGGCATCTTTATAGCGATAGCA‑ATTATTTACTGAGCGTCGCGACCATCACCGCTTTGATAGT   <  SRR4302537.3885781/94‑1 (MQ=38)
                                                                                              ATAATGTGATGCCGGGATGGTTTGTATTTCCCGGCATCTTTATAGCGATAGCA‑ATTATTTACTGAGCGTCGCGACCATCACCGCTTTGATAGTA  >  SRR4302537.5475734/1‑94 (MQ=38)
                                                                                              ATAATGTGATGCCGGGATGGTTTGTATTTCCCGGCATCTTTATAGCGATAGCA‑ATTATTTACTGAGCGTCGCGACCATCACCGCTTTGATAGTA  >  SRR4302537.2136905/1‑94 (MQ=38)
                                                                                              ATAATGTGATGCCGGGATGGTTTGTATTTCCCGGCATCTTTATAGCGATAGCA‑ATTATTTACTGAGCGTCGCGACCATCACCGCTTTGATAGTA  >  SRR4302537.366057/1‑94 (MQ=38)
                                                                                              ATAATGTGATGCCGGGATGGTTTGTATTTCCCGGCATCTTTATAGCGATAGCA‑ATTATTTACTGAGCGTCGCGACCATCACCGCTTTGATAGTA  >  SRR4302537.3692845/1‑94 (MQ=38)
                                                                                              |                                                                                              
AATGAACAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCAT‑AAATAATGTGATGCCGGGATGGTTT‑‑‑‑‑‑‑‑CCGCATC‑TTATAGCGAT‑‑CAGATTATTTACTGAGCGTCGCGACCATCACCGCTTTGATAGTA  >  NC_000913/4474733‑4474909

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑