Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 882 254.4 12957588 98.0% 12698436 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,479,401 T→C T669A (ACG→GCG)  valS ← valine tRNA synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,479,4010TC95.8% 688.7 / 10.9 216T669A (ACG→GCG) valSvaline tRNA synthetase
Reads supporting (aligned to +/- strand):  ref base T (4/5);  new base C (112/95);  total (116/100)
Fisher's exact test for biased strand distribution p-value = 7.36e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01

Only 100 of 229 total aligned reads displayed.

ATCGAAGCGGAAGCTGTCCAGCGCTTCGCGGTACGCTTTGATGGTCTGGTTGAACTCCGCCAGAATCCAGCGGTCCGCCAGCGACAGCGTCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCTTCTGTGTTCATCAGCACAAAGCGGCTGGCGTTCCACAGCTTGTTACAGAAGTTACGG  >  NC_000913/4479312‑4479492
                                                                                         |                                                                                           
atCGAAGCGGAAGCTGTCCAGCGCTTCGCGGTACGCTTTGATGGTCTGGTTGAACTCCGCCAGAATCCAGCGGTCCGCCAGCGACAGCGCCAt                                                                                          >  1:4576742/1‑93 (MQ=255)
 tCGAAGCGGAAGCTGTCCAGCGCTTCGCGGTACGCTTTGATGGTCTGGTTGAACTCCGCCAGAATCCAGCGGTCCGCCAGCGACAGCGCCAtt                                                                                         >  2:3706747/1‑93 (MQ=255)
 tCGAAGCGGAAGCTGTCCAGCGCTTCGCGGTACGCTTTGATGGTCTGGTTGAACTCCGCCAGAATCCAGCGGTCCGCCAGCGACAGCGCCAtt                                                                                         <  1:4929896/93‑1 (MQ=255)
   gAAGCGGAAGCTGTCCAGCGCTTCGCGGTACGCTTTGATGGTCTGGTTGAACTCCGCCAGAATCCAGCGGTCCGCCAGCGACAGCGCCATTTc                                                                                       >  1:5115020/1‑93 (MQ=255)
    aaGCGGAAGCTGTCCAGCGCTTCGCGGTACGCTTTGATGGTCTGGTTGAACTCCGCCAGAATCCAGCGGTCCGCCAGCGACAGCGCCATTTcg                                                                                      >  2:2714525/1‑93 (MQ=255)
     aGCGGAAGCTGTCCAGCGCTTCGCGGTACGCTTTGATGGTCTGGTTGAACTCCGCCAGAATCCAGCGGTCCGCCAGCGACAGCGCCATTTcgc                                                                                     >  1:4161964/1‑93 (MQ=255)
      gCGGAAGCTGTCCAGCGCTTCGCGGTACGCTTTGATGGTCTGGTTGAACTCCGCCAGAATCCAGCGGTCCGCCAGCGACAGCGCCATTTcgcc                                                                                    >  2:5303796/1‑93 (MQ=255)
        ggAAGCTGTCCAGCGCTTCGCGGTACGCTTTGATGGTCTGGTTGAACTCCGCCAGAATCCAGCGGTCCGCCAGCGACAGCGCCATTTcgccgc                                                                                  >  2:6124714/1‑93 (MQ=255)
         gAAGCTGTCCAGCGCTTCGCGGTACGCTTTGATGGTCTGGTTGAACTCCGCCAGAATCCAGCGGTCCGCCAGCGACAGCGCCATTTCgccgcc                                                                                 >  2:817948/1‑93 (MQ=255)
         gAAGCTGTCCAGCGCTTCGCGGTACGCTTTGATGGTCTGGTTGAACTCCGCCAGAATCCAGCGGTCCGCCAGCGACAGCGCCATTTCgccgcc                                                                                 <  2:4177923/93‑1 (MQ=255)
           aGCTGTCCAGCGCTTCGCGGTACGCTTTGATGGTCTGGTTGAACTCCGCCAGAATCCAGCGGTCCGCCAGCGACAGCGCCATTTCGCCGCCGt                                                                               >  1:3472029/1‑93 (MQ=255)
            gCTGTCCAGCGCTTCGCGGTACGCTTTGATGGTCTGGTTGAACTCCGCCAGAATCCAGCGGTCCGCCAGCGACAGCGCCATTTCGCCGCCGtt                                                                              >  2:5650727/1‑93 (MQ=255)
              tttCCAGCGCTTCGCGGTACGCTTTGATGGTCTGGTTGAACTCCGCCAGAATCCAGCGGTCCGCCAGCGACAGCGCCATTTCGCCGCCGTTGa                                                                            <  1:3705647/91‑1 (MQ=255)
              tGTCCAGCGCTTCGCGGTACGCTTTGATGGTCTGGTTGAACTCCGCCAGAATCCAGCGGTCCGCCAGCGACAGCGCCATTTCGCCGCCGTTGa                                                                            <  2:1790363/93‑1 (MQ=255)
              tGTCCAGCGCTTCGCGGTACGCTTTGATGGTCTGGTTGAACTCCGCCAGAATCCAGCGGTCCGCCAGCGACAGCGCCATTTCGCCGCCGTTGa                                                                            <  2:442712/93‑1 (MQ=255)
                tCCAGCGCTTCGCGGTACGCTTTGATGGTCTGGTTGAACTCCGCCAGAATCCAGCGGTCCGCCAGCGACAGCGCCATTTCGCCGCCGTTGAAg                                                                          >  2:1971904/1‑93 (MQ=255)
                 ccAGCGCTTCGCGGTACGCTTTGATGGTCTGGTTGAACTCCGCCAGAATCCAGCGGTCCGCCAGCGACAGCGCCATTTCGCCGCCGTTGAAGc                                                                         <  2:1058253/93‑1 (MQ=255)
                 ccAGCGCTTCGCGGTACGCTTTGATGGTCTGGTTGAACTCCGCCAGAATCCAGCGGTACGCCAGCGACAGCGCCATTTCGCCGCCGTTGAAGc                                                                         <  2:3539941/93‑1 (MQ=255)
                  cAGCGCTTCGCGGTACGCTTTGATGGTCTGGTTGAACTCCGCCAGAATCCAGCGGTCCGCCAGCGACAGCGCCATTTCGCCGCCGTTGAAGcc                                                                        >  2:3639401/1‑93 (MQ=255)
                   aGCGCTTCGCGGTACGCTTTGATGGTCTGGTTGAACTCCGCCAGAATCCAGCGGTCCGCCAGCGACAGCGCCATTTCGCCGCCGTTGAAGCCg                                                                       >  2:3386061/1‑93 (MQ=255)
                    gcgcTTCGCGGTACGCTTTGATGGTCTGGTTGAACTCCGCCAGAATCCAGCGGTCCGCCAGCGACAGCGCCATTTCGCCGCCGTTGAAGCCGc                                                                      <  1:4302230/93‑1 (MQ=255)
                     cgcTTCGCGGTACGCTTTGATGGTCTGGTTGAACTCCGCCAGAATCCAGCGGTCCGCCAGCGACAGCGCCATTTCGCCGCCGTTGAAGCCGCa                                                                     >  1:1689779/1‑93 (MQ=255)
                       cTTCGCGGTACGCTTTGATGGTCTGGTTGAACTCCGCCAGAATCCAGCGGTCCGCCAGCGACAGCGCCATTTCGCCGCCGTTGAAGCCGCAAt                                                                   <  1:3897045/93‑1 (MQ=255)
                          cgcgGTACGCTTTGATGGTCTGGTTGAACTCCGCCAGAATCCAGCGGTCCGCCAGCGACAGCGCCATTTCGCCGCCGTTGAAGCCGCAATCCt                                                                <  1:5916416/93‑1 (MQ=255)
                          cgcgGTACGCTTTGATGGTCTGGTTGAACTCCGCCAGAATCCAGCGGTCCGCCAGCGACAGCGCCATTTCGCCGCCGTTGAAGCCGCAATCCt                                                                <  2:5722469/93‑1 (MQ=255)
                           gcgGTACGCTTTGATGGTCTGGTTGAACTCCGCCAGAATCCAGCGGTCCGCCAGCGACAGCGCCATTTCGCCGCCGTTGAAGCCGCAATCCTg                                                               >  2:1592346/1‑93 (MQ=255)
                           gcgGTACGCTTTGATGGTCTGGTTGAACTCCGCCAGAATCCAGCGGTCCGCCAGCGACAGCGCCATTTCGCCGCCGTTGAAGCCGCAATCCTg                                                               >  1:4360299/1‑93 (MQ=255)
                           gcgGTACGCTTGGATGGTCTGGTTGAACTCCGCCAGAATCCAGCGGTCCGCCAGCGACAGCGCCATTTCGCCGCCGTTGAAGCCGCAATCCTg                                                               <  2:2540069/93‑1 (MQ=255)
                            cgGTACGCTTTGATGGTCTGGTTGAACTCCGCCAGAATCCAGCGGTCCGCCAGCGACAGCGTCATTTCGCCGCCGTTGAAGCCGCAATCCTGa                                                              <  1:5259901/93‑1 (MQ=255)
                             ggTACGCTTTGATGGTCTGGTTGAACTCCGCCAGAATCCAGCGGTCCGCCAGCGACAGCGCCATTTCGCCGCCGTTGAAGCCGCAATCCTGAc                                                             <  2:5156263/93‑1 (MQ=255)
                             ggTACGCTTTGATGGTCTGGTTGAACTCCGCCAGAATCCAGCGGTCCGCCAGCGACAGCGCCATTTCGCCGCCGTTGAAGCCGCAATCCTGAc                                                             >  1:2149788/1‑93 (MQ=255)
                             ggTACGCTTTGATGGTCTGGTTGAACTCCGCCAGAATCCAGCGGTCCGCCAGCGACAGCGCCATTTCGCCGCCGTTGAAGCCGCAATCCTGAc                                                             >  2:685585/1‑93 (MQ=255)
                               tACGCTTTGATGGTCTGGTTGAACTCCGCCAGAATCCAGCGGTCCGCCAGCGACAGCGCCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCt                                                           >  1:2047242/1‑93 (MQ=255)
                                 cGCTTTGATGGTCTGTTTGAACTCCGCCAGAATCCAGCGGTCCGCCAGCGACAGCGCCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCTTc                                                         <  2:3762337/93‑1 (MQ=255)
                                 cGCTTTGATGGTCTGGTTGAACTCCGCCAGAATCCAGCGGTCCGCCAGCGACAGCGCCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCTTc                                                         >  2:1358187/1‑93 (MQ=255)
                                 cGCTTTGATGGTCTGGTTGAACTCCGCCAGAATCCAGCGGTCCGCCAGCGACAGCGCCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCTTc                                                         <  1:2004275/93‑1 (MQ=255)
                                 cGCTTTGATGGTCTGGTTGAACTCCGCCAGAATCCAGCGGTCCGCCAGCGACAGCGCCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCTTc                                                         <  2:1101312/93‑1 (MQ=255)
                                 cGCTTTGATGGTCTGGTTGAACTCCGCCAGAATCCAGCGGTCCGCCAGCGACAGCGCCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCTTc                                                         <  2:1645866/93‑1 (MQ=255)
                                  gCTTTGATGGTCTGGTTGAACTCCGCCAGAATCCAGCGGTCCGCCAGCGACAGCGTCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCTTCt                                                        >  1:4016060/1‑93 (MQ=255)
                                    tttGATGGTCTGGTTGAACTCCGCCAGAATCCAGCGGTCCGCCAGCGACAGCGCCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCTTCtgt                                                      <  1:3564745/93‑1 (MQ=255)
                                      tGATGGTCTGGTTGACCTCCGCCAGAATCCAGCGGTCCGCCAGCGACAGCGCCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCTTCTgtgt                                                    <  2:2299043/93‑1 (MQ=255)
                                      tGATGGTCTGGTTGAACTCCGCCAGAATCCAGCGGTCCGCCAGCGACAGCGCCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCTTCTgtgt                                                    <  2:2054808/93‑1 (MQ=255)
                                        aTGGTCTGGTTGAACTCCGCCAGAATCCAGCGGTCCGCCAGCGACAGCGCCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCTTCTGTGTtc                                                  >  1:6182897/1‑93 (MQ=255)
                                         tGGTCTGGTTGAACTCCGCCAGAATCCAGCGGTCCGCCAGCGACAGCGCCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCTTCTGTGTtca                                                 >  1:3737535/1‑93 (MQ=255)
                                         tGGTCTGGTTGAACTCCGCCAGAATCCAGCGGTCCGCCAGCGACAGCGCCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCTTCTGTGTtca                                                 <  1:812183/93‑1 (MQ=255)
                                          ggTCTGGTTGAACTCCGCCAGAATCCAGCGGTCCGCCAGCGACAGCGCCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCTTCTGTGTtcat                                                >  1:3068756/1‑93 (MQ=255)
                                            tCTGTTTGAACTCCGCCAGAATCCAGCGGTCCGCCAGCGACAGCGCCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCTTCTGTGTtcatca                                              <  2:223588/93‑1 (MQ=255)
                                            tCTGGTTGAACTCCGCCAGAATCCAGCGGTCCGCCAGCGACAGCGCCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCTTCTGTGTtcatca                                              >  2:6169423/1‑93 (MQ=255)
                                            tCTGGTTGAACTCCGCCAGAATCCAGCGGTCCGCCAGCGACAGCGCCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCTTCTGTGTtcatca                                              <  2:527628/93‑1 (MQ=255)
                                             cTGGTTGACCCCCGCCAGAATCCAGCGGTCCGCCAGCGACAGCGCCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCTTCTGTGTTCATCAg                                             <  1:1773681/93‑1 (MQ=255)
                                               ggTTGAACTCCGCCAGAATCCAGCGGTCCGCCAGCGACAGCGCCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCTTCTGTGTTCATCAGca                                           <  2:4944929/93‑1 (MQ=255)
                                                gTTGAACTCCGCCAGAATCCAGCGGTCCGCCAGCGACAGCGCCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCTTCTGTGTTCATCAGcac                                          >  2:4283632/1‑93 (MQ=255)
                                                gTTGAACTCCGCCAGAATCCAGCGGTCCGCCAGCGACAGCGCCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCTTCTGTGTTCATCAGcac                                          >  2:4672418/1‑93 (MQ=255)
                                                  tGAACTCCGCCAGAATCCAGCGGTCCGCCAGCGACAGCGCCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCTTCTGTGTTCATCAGCACaa                                        >  2:632537/1‑93 (MQ=255)
                                                  tGAACTCCGCCAGAATCCAGCGGTCCGCCAGCGACAGCGCCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCTTCTGTGTTCATCAGCACaa                                        >  1:2881492/1‑93 (MQ=255)
                                                   gaccTCCGCCAGAATCCAGCGGTCCCCCAGCGACAGCGCCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCTTCTGTGTTCATCAGCACaaa                                       <  1:4734213/90‑1 (MQ=255)
                                                    accTCCGCCAGAATCCAGCGGTCCGCCAGCGACAGCGCCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCTTCTGTGTTCATCAGCACAAAg                                      <  2:966584/91‑1 (MQ=255)
                                                    aaCTCCGCCAGAATCCAGCGGTCCGCCAGCGACAGCGCCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCTTCTGTGTTCATCAGCACAAAg                                      <  2:6022932/93‑1 (MQ=255)
                                                     aCTCCGCCAGAATCCAGCGGTCCGCCAGCGACAGCGCCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCTTCTGTGTTCATCAGCACAAAGc                                     >  1:695612/1‑93 (MQ=255)
                                                     aCTCCGCCAGAATCCAGCGGTCCGCCAGCGACAGCGCCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCTTCTGTGTTCATCAGCACAAAGc                                     >  1:5038090/1‑93 (MQ=255)
                                                     aCTCCGCCAGAATCCAGCGGTCCGCCAGCGACAGCGCCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCTTCTGTGTTCATCAGCACAAAGc                                     >  2:3133281/1‑93 (MQ=255)
                                                     aCTCCGCCAGAATCCAGCGGTCCGCCAGCGACAGCGCCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCTTCTGTGTTCATCAGCACAAAGc                                     >  2:1162462/1‑93 (MQ=255)
                                                     aCTCCGCCAGAATCCAGCGGTCCGCCAGCGACAGCGCCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCTTCTGTGTTCATCAGCACAAAGc                                     >  1:1461856/1‑93 (MQ=255)
                                                      cTCCGCCAGAATCCAGCGGTCCGCCAGCGACAGCGCCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCTTCTGTGTTCATCAGCACAAAGCg                                    >  2:3224348/1‑93 (MQ=255)
                                                       tCCGCCAGAATCCAGCGGTCCGCCAGCGACAGCGCCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCTTCTGTGTTCATCAGCACAAAGCgg                                   >  1:2676385/1‑93 (MQ=255)
                                                        ccGCCAGAATCCAGCGGTCCGCCAGCGACAGCGCCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCTTCTGTGTTCATCAGCACAAAGCGGc                                  <  1:931828/93‑1 (MQ=255)
                                                        ccGCCAGAATCCAGCGGTCCGCCAGCGACAGCGCCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCTTCTGTGTTCATCAGCACAAAGCGGc                                  <  1:6236344/93‑1 (MQ=255)
                                                        ccGCCAGAATCCAGCGGTCCGCCAGCGACAGCGCCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCTTCTGTGTTCATCAGCACAAAGCGGc                                  <  2:5214066/93‑1 (MQ=255)
                                                          gCCAGAATCCAGCGGTCCGCCAGCGACAGCGCCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCTTCTGTGTTCATCAGCACAAAGCGGCTg                                <  1:29344/93‑1 (MQ=255)
                                                           ccAGAATCCAGCGGTCCGCCAGCGACAGCGCCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCTTCTGTGTTCATCAGCACACAGCGGCTgg                               >  2:2858616/1‑93 (MQ=255)
                                                            cAGAATCCAGCGGTCCGCCAGCGACAGCGCCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCTTCTGTGTTCATCAGCACAAAGCGGCTGGc                              <  1:277611/93‑1 (MQ=255)
                                                            cAGAATCCAGCGGTCCGCCAGCGACAGCGCCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCTTCTGTGTTCATCAGCACAAAGCGGCTGGc                              <  2:4881361/93‑1 (MQ=255)
                                                             aGAATCCAGCGGTCCGCCAGCGACAGCGCCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCTTCTGTGTTCATCAGCACAAAGCGGCTGGCg                             >  2:5799202/1‑93 (MQ=255)
                                                              gAATCCAGCGGTCCGCCAGCGACAGCGCCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCTTCTGTGTTCATCAGCACAAAGCGGCTGGCGt                            >  1:2373840/1‑93 (MQ=255)
                                                               aaTCCAGCGGTCCGCCAGCGACAGCGCCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCTTCTGTGTTCATCAGCACAAAGCGGCTGGCCtt                           >  1:1282168/1‑93 (MQ=255)
                                                                aTCCAGCGGTCCGCCAGCGACAGCGCCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCTTCTGTGTTCATCAGCACAAAGCGGCTGGCGTTc                          <  2:885053/93‑1 (MQ=255)
                                                                aTCCAGCGGTCCGCCAGCGACAGCGCCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCTTCTGTGTTCATCAGCACAAAGCGGCTGGCGTTc                          >  2:4191917/1‑93 (MQ=255)
                                                                 tCCAGCGGTCCGCCAGCGACAGCGCCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCTTCTGTGTTCATCAGCACAAAGCGGCTGGCGTTcc                         >  1:3548441/1‑93 (MQ=255)
                                                                  ccAGCGGTCCGCCAGCGACAGCGCCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCTTCTGTGTTCATCAGCACAAAGCGGCTGGCGTTCca                        >  1:5656028/1‑93 (MQ=255)
                                                                   cAGCGGTCCGCCAGCGACAGCGCCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCTTCTGTGTTCATCAGCACAAAGCGGCTGGGGTTCcac                       >  1:5566148/1‑93 (MQ=255)
                                                                   cAGCGGTCCGCCAGCGACAGCGCCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCTTCTGTGTTCATCAGCACAAAGCGGCTGGCGTTCcac                       <  1:904409/93‑1 (MQ=255)
                                                                     gCGGTCCGCCAGCGACAGCGCCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCTTCTGTGTTCATCAGCACAAAGCGGCTGGCGTTCCACAg                     >  1:3778190/1‑93 (MQ=255)
                                                                     gCGGTCCGCCAGCGACAGCGCCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCTTCTGTGTTCATCAGCACAAAGCGGCTGGCGTTCCACAg                     <  2:148391/93‑1 (MQ=255)
                                                                     gCGGTCCGCCAGCGACAGCGCCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCTTCTGTGTTCATCAGCACAAAGCGGCTGGCGTTCCACAg                     >  2:2285599/1‑93 (MQ=255)
                                                                     gCGGACCGCCAGCGACAGCGCCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCTTCTGTGTTCATCAGCACAAAGCGGCTGGCGTTCCACAg                     >  2:3472862/1‑93 (MQ=255)
                                                                      cGGTCCGCCAGCGACAGCGCCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCTTCTGTGTTCATCAGCACAAAGCGGCTGGCGTTCCACAGc                    <  1:144595/93‑1 (MQ=255)
                                                                       ggTCCGCCAGCGACAGCGCCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCTTCTGTGTTCATCAGCACAAAGCGGCTGGCGTTCCACAGCt                   <  2:1608851/93‑1 (MQ=255)
                                                                        gTCCGCCAGCGACAGCGCCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCTTCTGTGTTCATCAGCACAAAGCGGCTGGCGTTCCACAGCtt                  <  2:5568038/93‑1 (MQ=255)
                                                                        gTCCGCCAGCGACAGCGCCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCTTCTGTGTTCATCAGCACAAAGCGGCTGGCGTTCCACAGCtt                  >  2:715859/1‑93 (MQ=255)
                                                                        gTCCGCCAGCGACAGCGCCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCTTCTGTGTTCATCAGCACAAAGCGGCTGGCGTTCCACAGCtt                  >  1:6380233/1‑93 (MQ=255)
                                                                            gCCAGCGACAGCGCCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCTTCTGTGTTCATCAGCACAAAGCGGCTGGCGTTCCACAGCTTGTTa              >  1:2623597/1‑93 (MQ=255)
                                                                             ccAGCGACAGCGTCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCTTCTGTGTTCATCAGCACAAAGCGGCTGGCGTTCCACAGCTTGTTAc             <  2:3947291/93‑1 (MQ=255)
                                                                              cAGCGACAGCGCCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCTTCTGTGTTCATCAGCACAAAGCGGCTGGCGTTCCACAGCTTGTTACa            >  1:2727648/1‑93 (MQ=255)
                                                                                gCGACAGCGCCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCTTCTGTGTTCATCAGCACAAAGCGGCTGGCGTTCCACAGCTTGTTACAGa          >  1:5991441/1‑93 (MQ=255)
                                                                                gCGACAGCGCCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCTTCTGTGTTCATCAGCACAAAGCGGCTGGCGTTCCACAGCTTGTTACAGa          >  2:4109874/1‑93 (MQ=255)
                                                                                gCGACAGCGCCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCTTCTGTGTTCATCAGCACAAAGCGGCTGGCGTTCCACAGCTTGTTACAGa          >  1:5220634/1‑93 (MQ=255)
                                                                                   aCAGCGCCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCTTCTGTGTTCATCAGCACAAAGCGGCTGGCGTTCCACAGCTTGTTACAGAAGt       >  2:359311/1‑93 (MQ=255)
                                                                                     aGCGCCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCTTCTGTGTTCATCAGCACAAAGCGGCTGGCGTTCCACAGCTTGTTACAGAAGTTa     <  1:1130077/93‑1 (MQ=255)
                                                                                     aGCGCCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCTTCTGTGTTCATCAGCACAAAGCGGCTGGCGTTCCACAGCTTGTTACAGAAGTTa     <  1:1806606/93‑1 (MQ=255)
                                                                                      gcgcCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCTTCTGTGTTCATCAGCACAAAGCGGCTGGCGTTCCACAGCTTGTTACAGAAGTTAc    <  2:2182747/93‑1 (MQ=255)
                                                                                         |                                                                                           
ATCGAAGCGGAAGCTGTCCAGCGCTTCGCGGTACGCTTTGATGGTCTGGTTGAACTCCGCCAGAATCCAGCGGTCCGCCAGCGACAGCGTCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCTTCTGTGTTCATCAGCACAAAGCGGCTGGCGTTCCACAGCTTGTTACAGAAGTTACGG  >  NC_000913/4479312‑4479492

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CGATATCGAAGCGGAAGCTGTCCAGCGCTTCGCGGTACGCTTTGATGGTCTGGTTGAACTCCGCCAGAATCCAGCGGTCCGCCAGCGACAGCGTCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCTTCTGTGTTCATCAGCACAAAGCGGCTGGCGTTCCACAGCTTGTTACAGAAGTTACGGT  >  NC_000913/4479308‑4479493
                                                                                             |                                                                                            
CGATATCGAAGCGGAAGCTGTCCAGCGCTTCGCGGTACGCTTTGATGGTCTGGTTGAACTCCGCCAGAATCCAGCGGTCCGCCAGCGACAGCGC                                                                                              >  SRR4302537.5697593/1‑94 (MQ=60)
CGATATCGAAGCGGAAGCTGTCCAGCGCTTCGCGGTACGCTTTGATGGTCTGGTTGAACTCCGCCAGAATCCAGCGGTCCGCCAGCGACAGCGC                                                                                              >  SRR4302537.5447605/1‑94 (MQ=60)
 GATATCGAAGCGGAAGCTGTCCAGCGCTTCGCGGTACGCTTTGATGGTCTGGTTGAACTCCGCCAGAATCCAGCGGTCCGCCAGCGACAGCGCC                                                                                             >  SRR4302537.4301510/1‑94 (MQ=60)
 GATATCGAAGCGGAAGCTGTCCAGCGCTTCGCGGTACGCTTTGATGGTCTGGTTGAACTCCGCCAGAATCCAGCGGTCCGCCAGCGACAGCGCC                                                                                             >  SRR4302537.6294220/1‑94 (MQ=60)
 GATATCGAAGCGGAAGCTGTCCAGCGCTTCGCGGGACGCTTTGATGGTCTGGTTGAACTCCGCCAGAATCCAGCGGTCCGCCAGCGACAGCGCC                                                                                             >  SRR4302537.1631207/1‑94 (MQ=60)
  ATATCGAAGCGGAAGCTGTCCAGCGCTTAGCGGTACGCCTTGATGGTCTGGTTGAACTCCGCCAGCATCCAGCGGTCCGCCAGCGACAGCGCCA                                                                                            >  SRR4302537.3199165/1‑94 (MQ=60)
   TATCGAAGCGGAAGCTGTCCAGCGCTTCGCGGTACGCTTTGATGGTCTGGTTGAACTCCGCCAGAATCCAGCGGTCCGCCAGCGACAGCGCCAT                                                                                           >  SRR4302537.4608417/1‑94 (MQ=60)
    ATCGAAGCGGAAGCTGTCCAGCGCTTCGCGGTACGCTTTGATGGTCTGGTTGAACTCCGCCAGAATCCAGCGGTCCGCCAGCGACAGCGCCATT                                                                                          >  SRR4302537.2338549/1‑94 (MQ=60)
    ATCGAAGCGGAAGCTGTCCAGCGCTTCGCGGTACGCTTTGATGGTCTGGTTGAACTCCGCCAGAATCCAGCGGTCCGCCAGCGACAGCGCCATT                                                                                          >  SRR4302537.4252048/1‑94 (MQ=60)
    ATCGAAGCGGAAGCTGTCCAGCGCTTCGCGGTACGCTTTGATGGTCTGGTTGAACTCCGCCAGAATCCAGCGGTCCGCCAGCGACAGCGCCATT                                                                                          >  SRR4302537.4017302/1‑94 (MQ=60)
    ATCGAAGCGGAAGCTGTCCAGCGCTTCGCGGTACGCTTTGATGGTCTGGTTGAACTCCGCCAGAATCCAGCGGTCCGCCAGCGACAGCGCCATT                                                                                          <  SRR4302537.3528027/94‑1 (MQ=60)
     TCGAAGCGGAAGCTGTCCAGCGCTTCGCGGTACGCTTTGATGGTCTGGTTGAACTCCGCCAGAATCCAGCGGTCCGCCAGCGACAGCGCCATTT                                                                                         <  SRR4302537.4963878/94‑1 (MQ=60)
      CGAAGCGGAAGCTGTCCAGCGCTTCGCGGTACGCTTTGATGGTCTGGTTGAACTCCGCCAGAATCCAGCGGTCCGCCAGCGACAGCGCCATTTC                                                                                        >  SRR4302537.5150276/1‑94 (MQ=60)
      CGAAGCGGAAGCTGTCCAGAGCTTCGCGGTACGCTTTGATGGTCTGGTTGAACTCCGCCAGAATCCAGCGGTCCGCCAGCGACAGCGCCATTTC                                                                                        <  SRR4302537.4554838/94‑1 (MQ=60)
       GAAGCGGAAGCTGTCGAGCGCTTCGCGGTACGCTTTGATGGTCTGGTTGAACTCCGCCAGAATCCAGCGGTCCGCCAGCGACAGCGCCATTTCG                                                                                       <  SRR4302537.202123/94‑1 (MQ=60)
        AAGCGGAAGCTGTCCAGCGCTTCGCGGTACGCTTTGATGGTCTGGTTGAACTCCGCCAGAATCCAGCGGTCCGCCAGCGACAGCGCCATTTCGC                                                                                      >  SRR4302537.4191031/1‑94 (MQ=60)
         AGCGGAAGCTGTCCAGCGCTTCGCGGTACGCTTTGATGGTCTGGTTGAACTCCGCCAGAATCCAGCGGTCCGCCAGCGACAGCGCCATTTCGCC                                                                                     <  SRR4302537.892266/94‑1 (MQ=60)
          GCGGAAGCTGTCCAGCGCTTCGCGGTACGCTTTGATGGTCTGGTTGAACTCCGCCAGAATCCAGCGGTCCGCCAGCGACAGCGCCATTTCGCCG                                                                                    <  SRR4302537.5243953/94‑1 (MQ=60)
           CGGAAGCTGTCCAGCGCTTCGCGGTACGCTTTGATGGTCTGGTTGAACTCCGCCAGAATCCAGCGGTCCGCCAGCGACAGCGCCATTTCGCCGC                                                                                   >  SRR4302537.2923848/1‑94 (MQ=60)
            GGAAGCTGTCCAGCGCTTCGCGGTACGCTTTGATGGTCTGGTTGAACTCCGCCAGAATCCAGCGGTCCGCCAGCGACAGCGCCATTTCGCCGCC                                                                                  >  SRR4302537.3642539/1‑94 (MQ=60)
            GGAAGCTGTCCAGCGCTTCGCGGTACGCTTTGATGGTCTGGTTGAACTCCGCCAGAATCCAGCGGTCCGCCAGCGACAGCGCCATTTCGCCGCC                                                                                  >  SRR4302537.887776/1‑94 (MQ=60)
              AAGCTGTCCAGCGCTTCGCGGTACGCTTTGATGGTCTGGTTGAACTCCGCCAGAATCCAGCGGTCCGCCAGCGACAGCGCCATTTCGCCGCCGT                                                                                >  SRR4302537.3496599/1‑94 (MQ=60)
                  TTTCCAGCGCTTCGCGGTACGCTTTGATGGTCTGGTTGAACTCCGCCAGAATCCAGCGGTCCGCCAGCGACAGCGCCATTTCGCCGCCGTTGAA                                                                            <  SRR4302537.3731706/94‑1 (MQ=60)
                   GTCCAGCGCTTCGCGGTACGCTTTGATGGTCTGGTTGAACTCCGCCAGAATCCAGCGGTCCGCCAGCGACAGCGCCATTTCGCCGCCGTTGAAG                                                                           >  SRR4302537.2849933/1‑94 (MQ=60)
                   GTCCAGCGCTTCGCGGTACGCTTTGATGGTCTGGTTGAACTCCGCCAGAATCCAGCGGTCCGCCAGCGACAGCGCCATTTCGCCGCCGTTGAAG                                                                           >  SRR4302537.2630777/1‑94 (MQ=60)
                   GTCCAGCGCTTCGCGGTACGCTTTGATGGTCTGGTTGAACTCCGCCAGAATCCAGCGGTCCGCCAGCGACAGCGCCATTTCGCCGCCGTTGAAG                                                                           >  SRR4302537.913254/1‑94 (MQ=60)
                    TCCAGCGCTTCGCGGTACGCTTTGATGGTCTGGTTGAACTCCGCCAGAATCCAGCGGTCCGCCAGCGACAGCGCCATTTCGCCGCCGTTGAAGC                                                                          >  SRR4302537.3019132/1‑94 (MQ=60)
                     CCAGCGCTTCGCGGTACGCTTTGATGGTCTGGTTGAACTCCGCCAGAATCCAGCGGTCCGCCAGCGACAGCGCCATTTCGCCGCCGTTGAAGCC                                                                         <  SRR4302537.528329/94‑1 (MQ=60)
                        GCGCTTCGCGGTACGCTTTGATGGTCTGGTTGAACTCCGCCAGAATCCAGCGGTCCGCCAGCGACAGCGCCATTTCGCCGCCGTTGAAGCCGCA                                                                      >  SRR4302537.1701608/1‑94 (MQ=60)
                        GCGCTTCGCGGTACGCTTTGATGGTCTGGTTGAACTCCGCCAGAATCCAGCGGTCCGCCAGCGACAGCGCCATTTCGCCGCCGTTGAAGCCGCA                                                                      <  SRR4302537.4332127/94‑1 (MQ=60)
                           CTTCGCGGTACGCTTTGATGGTCTGGTTGAACTCCGCCAGAATCCAGCGGTCCGCCAGCGACAGCGCCATTTCGCCGCCGTTGAAGCCGCAATC                                                                   <  SRR4302537.3924290/94‑1 (MQ=60)
                           CTNCNCNGTACGCTTTGATGGTCGGGTTGAGCTCCGCCAGAATCCAGCGTTCCGCCAGCGCCAGCGCCATTTCGCCGCCGTTGAAGCCGCAATC                                                                   <  SRR4302537.2163027/94‑1 (MQ=60)
                             TCGCGTTACGCTTTGATGGTCTGGTTGAACTCCGCCAGAATCCAGCGGTCCGCCAGCGACAGCGCCATTTCGCCGCCGTTGAAGCCGCAATCCT                                                                 <  SRR4302537.4000499/94‑1 (MQ=60)
                             TCGCGGTACGCTTTGATGGTCTGGTTGAACTCCGCCAGAATCCAGCGGTCCGCCAGCGACAGCGCCATTTCGCCGCCGTTGAAGCCGCAATCCT                                                                 <  SRR4302537.5752339/94‑1 (MQ=60)
                              CGCGGTACGCTTTGATGGTCTGGTTGAACTCCGCCAGAATCCAGCGGTCCGCCAGCGACAGCGCCATTTCGCCGCCGTTGAAGCCGCAATCCTG                                                                <  SRR4302537.5957012/94‑1 (MQ=60)
                              CGCGGTACGCTTTGATGGTCTGGTTGAACTCCGCCAGAATCCAGCGGTCCGCCAGCGACAGCGCCATTTCGCCGCCGTTGAAGCCGCAATCCTG                                                                <  SRR4302537.5868095/94‑1 (MQ=60)
                              CGCGGTACGCTTTGATGGTCTGGTTGAACTCCGCCAGAATCCAGCGGTCCGCCAGCGACAGCGCCATTTCGCCGCCGTTGAAGCCGCAATCCTG                                                                <  SRR4302537.5271665/94‑1 (MQ=60)
                              CGCGGTACGCTTTGATGGTCTGGTTGAACTCCGCCAGAATCCAGCGGTCCGCCAGCGACAGCGCCATTTCGCCGCCGTTGAAGCCGCAATCCTG                                                                >  SRR4302537.4390472/1‑94 (MQ=60)
                                CGGTACGCTTTGATGGTCTGGTTGAACTCCGCCAGAATCCAGCGGTCCGCCAGCGACAGCGTCATTTCGCCGCCGTTGAAGCCGCAATCCTGAC                                                              <  SRR4302537.5296092/94‑1 (MQ=60)
                                CGGTACGCTTTGATGGTCTGGTTGAACTCCGCCAGAATCCAGCGGTCCGCCAGCGACAGCGCCATTTCGCCGCCGTTGAAGCCGCAATCCTGAC                                                              >  SRR4302537.2164940/1‑94 (MQ=60)
                                  GTACGCTTTGATGGTCTGGTTGAACTCCGCCAGAATCCAGCGGTCCGCCAGCGACAGCGCCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCT                                                            >  SRR4302537.2061596/1‑94 (MQ=60)
                                  GTACGCTTTGATGGTCTGGTTGAACTCCGCCAGAATCCAGCGGTCCGCCAGCGACAGCGCCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCT                                                            <  SRR4302537.2635893/94‑1 (MQ=60)
                                   TACGCTTTGATGGTCTGGTTGAACTCCGCCAGAATCCAGCGGTCCGCCAGCGACAGCGCCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCTT                                                           >  SRR4302537.636134/1‑94 (MQ=60)
                                     CGTTTTGATGGTCTGGTGGAACTCCGCCAGAATCCAGCGGTCCGCCAGCGACAGCGCCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCTTCT                                                         <  SRR4302537.4655051/94‑1 (MQ=60)
                                     CGCTTTGATGGTCTGGTTGAACTCCGCCAGAATCCAGCGGTCCGCCAGCGACAGCGTCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCTTCT                                                         >  SRR4302537.4044178/1‑94 (MQ=60)
                                     CGCTTTGATGGTCTGGTTGAACTCCGCCAGAATCCAGCGGTCCGCCAGCGACAGCGCCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCTTCT                                                         <  SRR4302537.1719904/94‑1 (MQ=60)
                                     CGCTTTGATGGTCTGGTTGAACTCCGCCAGAATCCAGCGGTCCGCCAGCGACAGCGCCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCTTCT                                                         <  SRR4302537.2018212/94‑1 (MQ=60)
                                     CGCTTTGATGGTCTGGTTGAACTCCGCCAGAATCCAGCGGTCCGCCAGCGACAGCGCCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCTTCT                                                         <  SRR4302537.5688158/94‑1 (MQ=60)
                                       CTTTGATGGTCTGGTTGAACTCCGCCAGAATCCAGCGGTCCGCCAGCGACAGCGCCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCTTCTGT                                                       <  SRR4302537.2790983/94‑1 (MQ=60)
                                        TTTGATGGTCTGGTTGAACTCCGCCAGAATCCAGCGGTCCGCCAGCGACAGCGCCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCTTCTGTG                                                      <  SRR4302537.3589888/94‑1 (MQ=60)
                                         TTGATGGTCTGGTTGACCTCCGCCAGAATCCAGCGGTCCGCCAGCGACAGCGCCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCTTCTGTGT                                                     <  SRR4302537.1143402/94‑1 (MQ=60)
                                          TGATGGTCTGGTTGAACTCCGCCAGAATCCAGCGGTCCGCCAGCGACAGCGCCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCTTCTGTGTT                                                    <  SRR4302537.3290355/94‑1 (MQ=60)
                                           GATGGTCTGGTTGAACTCCGCCAGAATCCAGCGGTCCGCCAGCGACAGCGCCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCTTCTGTGTTC                                                   >  SRR4302537.6225484/1‑94 (MQ=60)
                                           GATGGTCTGGTTGAACTCCGCCAGAATCCAGCGGTCCGCCAGCGACAGCGCCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCTTCTGTGTTC                                                   >  SRR4302537.6225485/1‑94 (MQ=60)
                                            ATGGTCTGGTTGAACTCCGCCAGAATCCAGCGGTCCGCCAGCGACAGCGCCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCTTCTGTGTTCA                                                  >  SRR4302537.6383369/1‑94 (MQ=60)
                                            ATGGTCTGGTTGAACTCCGCCAGAATCCAGCGGTCCGCCAGCGACAGCGCCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCTTCTGTGTTCA                                                  >  SRR4302537.3763779/1‑94 (MQ=60)
                                             TGGTCTGGTTGAACTCCGCCAGAATCCAGCGGTCCGCCAGCGACAGCGCCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCTTCTGTGTTCAT                                                 <  SRR4302537.817748/94‑1 (MQ=60)
                                             TGGTCTGGTTGAACTCCGCCAGAATCCAGCGGTCCGCCAGCGACAGCGCCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCTTCTGTGTTCAT                                                 >  SRR4302537.3090139/1‑94 (MQ=60)
                                               GTCTGGTTGAACTCCGCCAGAATCCAGCGGTCCGCCAGCGACAGCGCCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCTTCTGTGTTCATCA                                               >  SRR4302537.5594990/1‑94 (MQ=60)
                                                 CTGGTTGACCCCCGCCAGAATCCAGCGGTCCGCCAGCGACAGCGCCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCTTCTGTGTTCATCAGC                                             <  SRR4302537.1786034/94‑1 (MQ=60)
                                                    GTTGAACTCCGCCAGAATCCAGCGGTCCGCCAGCGACAGCGCCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCTTCTGTGTTCATCAGCACA                                          <  SRR4302537.1978052/94‑1 (MQ=60)
                                                     TTGAACTCCGCCAGAATCCAGCGGTCCGCCAGCGACAGCGCCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCTTCTGTGTTCATCAGCACAA                                         >  SRR4302537.2901717/1‑94 (MQ=60)
                                                      TGAACTCCGCCAGAATCCAGCGGTCCGCCAGCGACAGCGCAATTTCGCCGCCGTTGAAGCCGCAATCCTGACCTTCTGTGTTCATCAGCACAAA                                        >  SRR4302537.4153683/1‑94 (MQ=60)
                                                       TAACTCCGCCAGAATCTGGCGGTCCGCCAGCGGCAGCGCCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCTTCTGTGTTCATCAGCACAAAG                                       <  SRR4302537.1816434/94‑1 (MQ=60)
                                                       GACCTCCGCCAGAATCCAGCGGTCCCCCAGCGACAGCGCCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCTTCTGTGTTCATCAGCACAAAG                                       <  SRR4302537.4766928/94‑1 (MQ=60)
                                                        ACCTCCGCCAGAATCCAGCGGTCCGCCAGCGACAGCGCCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCTTCTGTGTTCATCAGCACAAAGC                                      <  SRR4302537.1626667/94‑1 (MQ=60)
                                                        AACTCCGCCAGAATCCAGCGGTCCGCCAGCGACAGCGCCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCTTCTGTGTTCATCAGCACAAAGC                                      >  SRR4302537.1472027/1‑94 (MQ=60)
                                                        AACTCCGCCAGAATCCAGCGGTCCGCCAGCGACAGCGCCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCTTCTGTGTTCATCAGCACAAAGC                                      >  SRR4302537.700382/1‑94 (MQ=60)
                                                        AACTCCGCCAGAATCCAGCGGTCCGCCAGCGACAGCGCCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCTTCTGTGTTCATCAGCACAAAGC                                      >  SRR4302537.3870817/1‑94 (MQ=60)
                                                        AACTCCGCCAGAATCCAGCGGTCCGCCAGCGACAGCGCCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCTTCTGTGTTCATCAGCACAAAGC                                      >  SRR4302537.5072773/1‑94 (MQ=60)
                                                         cgTCCGCCAGAATCCAGCGGTCCGCCAGCGACAGCGCCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCTTCTGTGTTCATCAGCACAAAGCG                                     <  SRR4302537.3800962/92‑1 (MQ=60)
                                                         ACTCCGCCAGAATCCAGCGGTCCGCCAGCGACAGCGCCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCTTCTGTGTTCATCAGCACAAAGCG                                     >  SRR4302537.4339606/1‑94 (MQ=60)
                                                          CTCCGCCAGAATCCAGCGGTCCGCCAGCGACAGCGCCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCTTCTGTGTTCATCAGCACAAAGCGG                                    >  SRR4302537.2695074/1‑94 (MQ=60)
                                                          CTCCGCCAGAATCCAGCGGTCCGCCAGCGACAGCGCCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCTTCTGTGTTCATCAGCACAAAGCGG                                    >  SRR4302537.3747169/1‑94 (MQ=60)
                                                          CTCCGCCAGAATCCAGCGGTCCGCCAGCGACAGCGCCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCTTCTGTGTTCATCAGCACAAAGCGG                                    <  SRR4302537.481182/94‑1 (MQ=60)
                                                            CCGCCAGAATCCAGCGGTCCGCCAGCGACAGCGCCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCTTCTGTGTTCATCAGCACAAAGCGGCT                                  <  SRR4302537.6279231/94‑1 (MQ=60)
                                                            CCGCCAGAATCCAGCGGTCCGCCAGCGACAGCGCCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCTTCTGTGTTCATCAGCACAAAGCGGCT                                  <  SRR4302537.938254/94‑1 (MQ=60)
                                                            CCCCCAGAATCCAGCGGTCCGCCAGCGACAGCGCCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCTTCTGTGTTCATCAGCACAAAGCGGCT                                  <  SRR4302537.3579080/94‑1 (MQ=60)
                                                              GCCAGAATCCAGCGGTCCGCCAGCGACAGCGCCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCTTCTGTGTTCATCAGCACAAAGCGGCTGG                                <  SRR4302537.29582/94‑1 (MQ=60)
                                                               CCAGAATCCAGCGGTCCGCCAGCGACAGCGCCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCTTCTGTGTTCATCAGCACAAAGCGGCTGGC                               <  SRR4302537.3282217/94‑1 (MQ=60)
                                                                CAGAATCCAGCGGTCCGCCAGCGACAGCGCCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCTTCTGTGTTCATCAGCACAAAGCGGCTGGCG                              >  SRR4302537.74174/1‑94 (MQ=60)
                                                                CAGAATCCAGCGGTCCGCCAGCGACAGCGCCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCTTCTGTGTTCATCAGCACAAAGCGGCTGGCG                              <  SRR4302537.279552/94‑1 (MQ=60)
                                                                 AGAATCCAGCGGTCCGCCAGCGACAGCGCCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCTTCTGTGTTCATCAGCACAAAGCGGCTGGCGT                             <  SRR4302537.1366969/94‑1 (MQ=60)
                                                                 AGAATCCAGCGGTCCGCCAGCGACAGCGCCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCTTCTGTGTTCATCAGCACAAAGCGGCTGGCGT                             >  SRR4302537.2390565/1‑94 (MQ=60)
                                                                 AGAATCCAGCGGTCCGCCAGCGACAGCGCCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCTTCTGTGTTCATCAGCACAAAGCGGCTGGCGT                             >  SRR4302537.6043251/1‑94 (MQ=60)
                                                                 AGAATCCAGCGGTCCGCCAGCGACAGCGCCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCTTCTGTGTTCATCAGCACAAAGCGGCTGGCGT                             >  SRR4302537.1581418/1‑94 (MQ=60)
                                                                  GAATCCAGCGGTCCGCCAGCGACAGCGCCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCTTCTGTGTTCATCAGCACAAAGCGGCTGGCCTT                            >  SRR4302537.1291081/1‑94 (MQ=60)
                                                                   AATCCAGCGGTCCGCCAGCGACAGCGCCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCTTCTGTGTTCATCAGCACAAAGCGGCTGGCGTTC                           >  SRR4302537.2686266/1‑94 (MQ=60)
                                                                    ATCCAGCGGTCCGCCAGCGACAGCGCCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCTTCTGTGTTCATCAGCACAAAGCGGCTGGCGTTCC                          >  SRR4302537.3573508/1‑94 (MQ=60)
                                                                     TCCAGCGGTCCGCCAGCGACAGCGCCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCTTCTGTGTTCATCAGCACAAAGCGGCTGGCGTTCCA                         >  SRR4302537.5694732/1‑94 (MQ=60)
                                                                      CCAGCGGTCCGCCAGCGACAGCGCCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCTTCTGTGTTCATCAGCACAAAGCGGCTGGGGTTCCAC                        >  SRR4302537.5604192/1‑94 (MQ=60)
                                                                       CAGCGGTCCGCCAGCGACAGCGCCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCTTCTGTGTTCATCAGCACAAAGCGGCTGGCGTTCCACA                       <  SRR4302537.910652/94‑1 (MQ=60)
                                                                        AGCGGTCCGCCAGCGACAGCGTCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCTTCTGTGTTCATCAGCACAAAGCGGCTGGCGTTCCACAG                      >  SRR4302537.693546/1‑94 (MQ=60)
                                                                        AGCGGTCCGCCAGCGACAGCGCCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCTTCTGTGTTCATCAGCACAAAGCGGCTGGCGTTCCACAG                      >  SRR4302537.3804721/1‑94 (MQ=60)
                                                                          CGGTCCGCCAGCGACAGCGCCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCTTCTGTGTTCATCAGCACAAAGCGGCTGGCGTTCCACAGCT                    <  SRR4302537.145625/94‑1 (MQ=60)
                                                                           GGTCCGCCAGCGACAGCGCCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCTTCTGTGTTCATCAGCACAAAGCGGCTGGCGTTCCACAGCTT                   <  SRR4302537.4370843/94‑1 (MQ=60)
                                                                           GGTCCGCCAGCGACAGCGCCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCTTCTGTGTTCATCAGCACAAAGCGGCTGGCGTTCCACAGCTT                   >  SRR4302537.6423733/1‑94 (MQ=60)
                                                                               CGCCAGCGACAGCGCCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCTTCTGTGTTCATCAGCACAAAGCGGCTGGCGTTCCACAGCTTGTTA               >  SRR4302537.2641985/1‑94 (MQ=60)
                                                                                GCCAGCGACAGCGCCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCTTCTGTGTTCATCAGCACAAAGCGGCTGGCGTTCCACAGCTTGTTAC              >  SRR4302537.2721658/1‑94 (MQ=60)
                                                                                GCCAGCGACAGCGCCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCTTCTGTGTTCATCAGCACAAAGCGGCTGGCGTTCCACAGCTTGTTAC              >  SRR4302537.1921064/1‑94 (MQ=60)
                                                                                 CCAGCGACAGCGCCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCTTCTGTGTTCATCAGCACAAAGCGGCTGGCGTTCCACAGCTTGTTACA             >  SRR4302537.2746692/1‑94 (MQ=60)
                                                                                 CCAGCGACAGCGCCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCTTCTGTGTTCATCAGCACAAAGCGGCTGGCGTTCCACAGCTTGTTACA             >  SRR4302537.2145023/1‑94 (MQ=60)
                                                                                  CAGCGACAGCGCCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCTTCTGTGTTCATCAGCACAAAGCGGCTGGCGTTCCACAGCTTGTTACAG            >  SRR4302537.6014683/1‑94 (MQ=60)
                                                                                   AGCGACAGCGCCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCTTCTGTGTTCATCAGCACAAAGCGGCTGGCGTTCCACAGCTTGTTACAGA           >  SRR4302537.6032589/1‑94 (MQ=60)
                                                                                   AGCGACAGCGCCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCTTCTGTGTTCATCAGCACAAAGCGGCTGGCGTTCCACAGCTTGTTACAGA           >  SRR4302537.5256536/1‑94 (MQ=60)
                                                                                    GCGACAGCGCCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCTTCTGTGTTCATCAGCACAAAGCGGCTGGCGTTCCACAGCTTGTTACAGAA          <  SRR4302537.3727614/94‑1 (MQ=60)
                                                                                     CGACAGCGTCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCTTCTGTGTTCATCAGCACAAAGCGGCTGGCGTTCCACAGCTTGTTACAGAAG         <  SRR4302537.1466997/94‑1 (MQ=60)
                                                                                        CAGCGCCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCTTCTGTGTTCATCAGCACAAAGCGGCTGGCGTTCCACAGCTTGTTACAGAAGTTA      <  SRR4302537.5129765/94‑1 (MQ=60)
                                                                                         AGCGCCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCTTCTGTGTTCATCAGCACAAAGCGGCTGGCGTTCCACAGCTTGTTACAGAAGTTAC     <  SRR4302537.1819211/94‑1 (MQ=60)
                                                                                         AGCGCCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCTTCTGTGTTCATCAGCACAAAGCGGCTGGCGTTCCACAGCTTGTTACAGAAGTTAC     <  SRR4302537.1137917/94‑1 (MQ=60)
                                                                                         AGCGCCATTTCGCCGCCGTGGAAGCCGCAATCCTGACCTTCTGTGTTCATCAGCACAAAGCGGCTGGCGTTCCACAGCTTGTTACAGAAGTTAC     <  SRR4302537.4969588/94‑1 (MQ=60)
                                                                                          GCGCCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCTTCTGTGTTCATCAGCACAAAGCGGCTGGCGTTCCACAGCTTGTTACAGAAGTTACG    >  SRR4302537.1781700/1‑94 (MQ=60)
                                                                                          GCGCCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCTTCTGTGTTCATCAGCACAAAGCGGCTGGCGTTCCACAGCTTGTTACAGAAGTTACG    <  SRR4302537.2793429/94‑1 (MQ=60)
                                                                                          GCGCCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCTTCTGTGTTCATCAGCACAAAGCGGCTGGCGTTCCACAGCTTGTTACAGAAGTTACG    <  SRR4302537.4978325/94‑1 (MQ=60)
                                                                                           CGCCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCTTCTGTGTTCATCAGCACAAAGCGGCTGGCGTTCCACAGCTTGTTACAGAAGTTACGG   >  SRR4302537.1098534/1‑94 (MQ=60)
                                                                                            GCCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCTTCTGTGTTCATCAGCACAAAGCGGCTGGCGTTCCACAGCTTGTTACAGAAGTTACTGT  >  SRR4302537.4893043/1‑94 (MQ=60)
                                                                                            GCCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCTTCTGTGTTCATCAGCACAAAGCGGCTGGCGTTCCACAGCTTGTTACAGAAGTTACGGT  <  SRR4302537.5622467/94‑1 (MQ=60)
                                                                                             |                                                                                            
CGATATCGAAGCGGAAGCTGTCCAGCGCTTCGCGGTACGCTTTGATGGTCTGGTTGAACTCCGCCAGAATCCAGCGGTCCGCCAGCGACAGCGTCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCTTCTGTGTTCATCAGCACAAAGCGGCTGGCGTTCCACAGCTTGTTACAGAAGTTACGGT  >  NC_000913/4479308‑4479493

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑