Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	882	254.4	12957588	98.0%	12698436	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,484,334	A→G	A183A (GCA→GCG)	yjgP →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,484,334	0	A	G	100.0%	772.1 / NA	222	A183A (GCA→GCG)	yjgP	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (115/107); total (115/107)

Only 100 of 231 total aligned reads displayed.

TAATGGCAGCTCGGTGCTGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCACGTCCTTCTGTGGTGGTGGCCGATTCCGGACATTTAACCCAGCTGCGCGACGGCTCCCAGGTCGTCACTCTCAACCAGGGAACGCGCTT  >  NC_000913/4484244‑4484423

tAATGGCAGCTCGGTGCTGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGcgcg                                                                                         >  1:3382605/1‑93 (MQ=255)

 aaTGGCAGCTCGGTGCTGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCGCGt                                                                                        >  1:4291102/1‑93 (MQ=255)

  aTGGCAGCTCGGTGCTGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCGCGTc                                                                                       <  2:3441502/93‑1 (MQ=255)

  aTGGCAGCTCGGTGCTGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCGCGTc                                                                                       >  2:1930418/1‑93 (MQ=255)

   tGGCAGCTCGGTGCTGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCGCGTcc                                                                                      <  2:367249/93‑1 (MQ=255)

     gCAGCTCGGTGCTGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCGCGTCCtt                                                                                    <  2:228036/93‑1 (MQ=255)

     gCAGCTCGGTGCTGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCGCGTCCtt                                                                                    <  1:1929948/93‑1 (MQ=255)

     gCAGCTCGGTGCTGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCGCGTCCtt                                                                                    >  2:406472/1‑93 (MQ=255)

      cAGCTCGGTGCTGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCGCGTCCTTc                                                                                   <  2:2669886/93‑1 (MQ=255)

      cAGCTCGGTGCTGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCGCGTCCTTc                                                                                   <  2:5774663/93‑1 (MQ=255)

       aGCTCGGTGCTGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCGCGTCCTTCt                                                                                  <  2:5484415/93‑1 (MQ=255)

       aGCTCGGTGCTGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCGCGTCCTTCt                                                                                  <  1:4962524/93‑1 (MQ=255)

        gCTCGGTGCTGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCGCGTCCTTCtg                                                                                 <  1:2573534/93‑1 (MQ=255)

          tCGGTGCTGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCGCGTCCTTCtgtg                                                                               >  1:4787296/1‑93 (MQ=255)

             gTGCTGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCGCGTCCTTCTgtggtg                                                                            >  2:2514355/1‑93 (MQ=255)

              tGCTGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCGCGTCCTTCTgtggtgg                                                                           <  1:5325197/93‑1 (MQ=255)

               gCTGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCGCGTCCTTCTgtggtggt                                                                          <  2:628907/93‑1 (MQ=255)

               gCTGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCGCGTCCTTCTgtggtggt                                                                          <  1:5181549/93‑1 (MQ=255)

               gCTGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCGCGTCCTTCTgtggtggt                                                                          >  1:3066001/1‑93 (MQ=255)

                cTGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCGCGTCCTTCTgtggtggtg                                                                         <  2:5766518/93‑1 (MQ=255)

                cTGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCGCGTCCTTCTgtggtggtg                                                                         >  2:454183/1‑93 (MQ=255)

                 tGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCGCGTCCTTCTGtggtggtgg                                                                        <  1:590809/93‑1 (MQ=255)

                  gTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCGCGTCCTTCTGTGGTGGTGGc                                                                       >  1:6066735/1‑93 (MQ=255)

                  gTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCGCGTCCTTCTGTGGTGGTGGc                                                                       <  1:1821103/93‑1 (MQ=255)

                   ttCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCGCGTCCTTCTGTGGTGGTGGcc                                                                      >  1:4613221/1‑93 (MQ=255)

                   ttCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCGCGTCCTTCTGTGGTGGTGGcc                                                                      >  1:145026/1‑93 (MQ=255)

                    tCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCGCGTCCTTCTGTGGTGGTGGCCg                                                                     <  2:2614119/93‑1 (MQ=255)

                     cATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCGCGTCCTTCTGTGGTGGTGGCCGa                                                                    <  2:3905821/93‑1 (MQ=255)

                      aTCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCGCGTCCTTCTGTGGTGGTGGCCGAt                                                                   <  1:701854/93‑1 (MQ=255)

                       tCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCGCGTCCTTCTGTGGTGGTGGCCGAtt                                                                  >  2:6104156/1‑93 (MQ=255)

                       tCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCGCGTCCTTCTGTGGTGGTGGCCGAtt                                                                  >  2:1310563/1‑93 (MQ=255)

                       tCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCGCGTCCTTCTGTGGTGGTGGCCGAtt                                                                  >  1:4737980/1‑93 (MQ=255)

                       tCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCGCGTCCTTCTGTGGTGGTGGCCGAtt                                                                  >  2:59864/1‑93 (MQ=255)

                         gAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCGCGTCCTTCTGTGGTGGTGGCCGATTcc                                                                <  2:2806027/93‑1 (MQ=255)

                          aaaGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCGCGTCCTTCTGTGGTGGTGGCCGATTCCg                                                               <  2:2353578/93‑1 (MQ=255)

                          aaaGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCGCGTCCTTCTGTGGTGGTGGCCGATTCCg                                                               >  1:3465035/1‑93 (MQ=255)

                           aaGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCGCGTCCTTCTGTGGTGGTGGCCGATTCCgg                                                              >  2:3099258/1‑93 (MQ=255)

                            aGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCGCGTCCTTCTGTGGTGGTGGCCGATTCCGGa                                                             <  1:5471654/93‑1 (MQ=255)

                            aGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCGCGTCCTTCTGTGGTGGTGGCCGATTCCGGa                                                             >  2:1414985/1‑93 (MQ=255)

                             gCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCGCGTCCTTCTGTGGTGGTGGCCGATTCCGGAc                                                            >  2:1900582/1‑93 (MQ=255)

                               gTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCGCGTCCTTCTGTGGTGGTGGCCGATTCCGGACAt                                                          >  2:735441/1‑93 (MQ=255)

                               gTTGACGGCAGCGATCTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCGCGTCCTTCTGTGGTGGTGGCCGATTCCGGACAt                                                          >  1:4111770/1‑93 (MQ=255)

                                   aCGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCGCGTCCTTCTGTGGTGGTGGCCGATTCCGGACATTTaa                                                      <  1:4431647/93‑1 (MQ=255)

                                    cGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCGCGTCCTTCTGTGGTGGTGGCCGATTCCGGACATTTAAc                                                     <  1:5617974/93‑1 (MQ=255)

                                    cGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCGCGTCCTTCTGTGGTGGTGGCCGATTCCGGACATTTAAc                                                     >  2:1821089/1‑93 (MQ=255)

                                     ggCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCGCGTCCTTCTGTGGTGGTGGCCGATTCCGGACATTTAAcc                                                    >  2:3627743/1‑93 (MQ=255)

                                      gCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCGCGTCCTTCTGTGGTGGTGGCCGATTCCGGACATTTAAccc                                                   >  2:439136/1‑93 (MQ=255)

                                      gCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCGCGTCCTTCTGTGGTGGTGGCCGATTCCGGACATTTAAccc                                                   >  1:1358658/1‑93 (MQ=255)

                                      gCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCGCGTCCTTCTGTGGTGGTGGCCGATTCCGGACATTTAAccc                                                   >  1:4860754/1‑93 (MQ=255)

                                       cAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGCCCAAAAGGTAATGCGCGTCCTTCTGTGGTGGTGGCCGATTCCGGACATTTAACCCa                                                  <  1:76742/93‑1 (MQ=255)

                                       cAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCGCGTCCTTCTGTGGTGGTGGCCGATTCCGGACATTTAACCCa                                                  <  1:2941472/93‑1 (MQ=255)

                                       cAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCGCGTCCTTCTGTGGTGGTGGCCGATTCCGGACATTTAACCCa                                                  <  1:2841101/93‑1 (MQ=255)

                                           gATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCGCGTCCTTCTGTGGTGGTGGCCGATTCCGGACATTTAACCCAGCTg                                              <  2:3496064/93‑1 (MQ=255)

                                            aTTTCAAAGATGTGTTCCTCGCGCAAGTTCGACCAAAAGTTAATGCGCGTCCTTCTGTGGTGGTGGCCGATTCCGGACATTTAACCCAGCTgc                                             <  2:652776/93‑1 (MQ=255)

                                            aTTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCGCGTCCTTCTGTGGTGGTGGCCGATTCCGGACATTTAACCCAGCTgc                                             >  1:4207716/1‑93 (MQ=255)

                                            aTTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCGCGTCCTTCTGTGGTGGTGGCCGATTCCGGACATTTAACCCAGCTgc                                             >  2:5160968/1‑93 (MQ=255)

                                             tttCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCGCGTCCTTCTGTGGTGGTGGCCGATTCCGGACATTTAACCCAGCTgcg                                            >  2:1086527/1‑93 (MQ=255)

                                             tttCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCGCGTCCTTCTGTGGTGGTGGCCGATTCCGGACATTTAACCCAGCTgcg                                            >  1:5526870/1‑93 (MQ=255)

                                                cAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCGCGTCCTTCTGTGGTGGTGGCCGATTCCGGACATTTAACCCAGCTGCGCGa                                         >  1:371334/1‑93 (MQ=255)

                                                cAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCGCGTCCTTCTGTGGTGGTGGCCGATTCCGGACATTTAACCCAGCTGCGCGa                                         <  1:2747334/93‑1 (MQ=255)

                                                 aaaGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCGCGTCCTTCTGTGGTGGTGGCCGATTCCGGACATTTAACCCAGCTGCGCGAc                                        >  1:5965756/1‑93 (MQ=255)

                                                   aGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCGCGTCCTTCTGTGGTGGTGGCCGATTCCGGACATTTAACCCAGCTGCGCGACgg                                      >  1:1305080/1‑93 (MQ=255)

                                                   aGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCGCGTCCTTCTGTGGTGGTGGCCGATTCCGGACATTTAACCCAGCTGCGCGACgg                                      <  2:4859294/93‑1 (MQ=255)

                                                    gATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCGCGTCCTTCTGTGGTGGTGGCCGATTCCGGACATTTAACCCAGCTGCGCGACGGc                                     >  2:4680302/1‑93 (MQ=255)

                                                     aTGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCGCGTCCTTCTGTGGTGGTGGCCGATTCCGGACATTTAACCCAGCTGCGCGACGGCt                                    <  2:6219811/93‑1 (MQ=255)

                                                        tgtTCCTCGCGCAAATTCGACCAAAAGGTAATGAGCGTCCTTCTGTGGTGGTGGCCGATTCCGGACATTTAACCCAGCTGCGCGATGGCTccc                                 >  1:4161954/1‑93 (MQ=255)

                                                         gtTCCTCGCGCAAATTCGACCAAAAGGTAATGCGCGTCCTTCTGTGGTGGTGGCCGATTCCGGACATTTAACCCAGCTGCGCGACGGCTCCCa                                >  1:1004556/1‑93 (MQ=255)

                                                         gtTCCTCGCGCAAATTCGACCAAAAGGTAATGCGCGTCCTTCTGTGGTGGTGGCCGATTCCGGACATTTAACCCAGCTGCGCGACGGCTCCCa                                >  2:6259126/1‑93 (MQ=255)

                                                         gtTCCTCGCGCAAATTCGACCAAAAGGTAATGCGCGTCCCTCTGTGGTGGTGGCCGATTCCGGACATTTAACCCAGCTGCGCGACGGCTCCCa                                <  1:6173693/93‑1 (MQ=255)

                                                          ttCCTCGCGCAAATTCGACCAAAAGGTAATGCGCGTCCTTCTGTGGTGGTGGCCGATTCCGGACATTTAACCCAGCTGCGCGACGGCTCCCAg                               >  2:3532401/1‑93 (MQ=255)

                                                           tCCTCGCGCAAATTCGACCAAAAGGTAATGCGCGTCCTTCTGTGGTGGTGGCCGATTCCGGACATTTAACCCAGCTGCGCGACGGCTCCCAgg                              <  1:3243858/93‑1 (MQ=255)

                                                              tCGCGCAAATTCGACCAAAAGGTAATGCGCGTCCTTCTGTGGTGGTGGCCGATTCCGGACATTTAACCCAGCTGCGCGACGGCTCCCAGgtcg                           >  2:3931879/1‑93 (MQ=255)

                                                              tCGCGCAAATTCGACCAAAAGGTAATGCGCGTCCTTCTGTGGTGGTGGCCGATTCCGGACATTTAACCCAGCTGCGCGACGGCTCCCAGgccg                           >  1:5216843/1‑93 (MQ=255)

                                                                      aTTCGACCAAAAGGTAATGCGCGTCCTTCTGTGGTGGTGGCCGATTCCGGACATTTAACCCAGCTGCGCGACGGCTCCCAGGTCGTCACtctc                   >  1:1106201/1‑93 (MQ=255)

                                                                          gACCAAAAGGTAATGCGCGTCCTTCTGTGGTGGTGGCCGATTCCGGACATTTAACCCAGCTGCGCGACGGCTCCCAGGTCGTCACTCTCAAcc               >  1:6287177/1‑93 (MQ=255)

                                                                          gACCAAAAGGTAATGCGCGTCCTTCTGTGGTGGTGGCCGATTCCGGACATTTAACCCAGCTGCGCGACGGCTCCCAGGTCGTCACTCTCAAcc               <  1:4359228/93‑1 (MQ=255)

                                                                           aCCAAAAGGTAATGCGCGTCCTTCTGTGGTGGTGGCCGATTCCGGACATTTAACCCAGCTGCGCGACGGCTCCCAGGTCGTCACTCTCAACCa              >  2:5448410/1‑93 (MQ=255)

                                                                            ccAAAAGGTAATGCGCGTCCTTCTGTGGTGGTGGCCGATTCCGGACATTTAACCCAGCTGCGCGACGGCTCCCAGGTCGTCACTCTCAACCAg             <  2:445354/93‑1 (MQ=255)

                                                                            ccAAAAGGTAATGCGCGTCCTTCTGTGGTGGTGGCCGATTCCGGACATTTAACCCAGCTGCGCGACGGCTCCCAGGTCGTCACTCTCAACCAg             >  2:2109548/1‑93 (MQ=255)

                                                                             cAAAAGGTAATGCGCGTCCTTCTGTGGTGGTGGCCGATTCCGGACATTTAACCCAGCTGCGCGACGGCTCCCAGGTCGTCACTCTCAACCAgg            >  1:5940162/1‑93 (MQ=255)

                                                                             cAAAAGGTAATGCGCGTCCTTCTGTGGTGGTGGCCGATTCCGGACATTTAACCCAGCTGCGCGACGGCTCCCAGGTCGTCACTCTCAACCAgg            >  2:5820420/1‑93 (MQ=255)

                                                                             cAAAAGGTAATGCGCGTCCTTCTGTGGTGGTGGCCGATTCCGGACATTTAACCCAGCTGCGCGACGGCTCCCAGGTCGTCACTCTCAACCAgg            >  1:116765/1‑93 (MQ=255)

                                                                              aaaaGGTAATGCGCGTCCTTCTGTGGTGGTGGCCGATTCCGGACATTTAACCCAGCTGCGCGACGGCTCCCAGGTCGTCACTCTCAACCAggg           <  1:3962573/93‑1 (MQ=255)

                                                                              aaaaGGTAATGCGCGTCCTTCTGTGGTGGTGGCCGATTCCGGACATTTAACCCAGCTGCGCGACGGCTCCCAGGTCGTCACTCTCAACCAggg           <  2:2896247/93‑1 (MQ=255)

                                                                               aaaGGTAATGCGCGTCCTTCTGTGGTGGTGGCCGATTCCGGACATTTAACCCAGCTGCGCGACGGCTCCCAGGTCGTCACTCTCAACCAGGGa          >  2:4015604/1‑93 (MQ=255)

                                                                                aaGGTAATGCGCGTCCTTCTGTGGTGGTGGCCGATTCCGGACATTTAACCCAGCTGCGCGACGGCTCCCAGGTCGTCACTCTCAACCAGGGaa         <  2:4106382/93‑1 (MQ=255)

                                                                                aaGGTAATGCGCGTCCTTCTGTGGTGGTGGCCGATTCCGGACATTTAACCCAGCTGCGCGACGGCTCCCAGGTCGTCACTCTCAACCAGGGaa         <  1:2917596/93‑1 (MQ=255)

                                                                                 aGGTAATGCGCGTCCTTCTGTGGTGGTGGCCGATTCCGGACATTTAACCCAGCTGCGCGACGGCTCCCAGGTCGTCACTCTCAACCAGGGAAc        <  1:2202173/93‑1 (MQ=255)

                                                                                 aGGTAATGCGCGGCCTTCTGTGGTTGTGGCCGATTCCGGACATTTAACCCAGCTGCGCGACGGCTCCCAGGTCGTCACTCTCAACCAGGGAAc        <  1:1059681/93‑1 (MQ=255)

                                                                                  ggTAATGCGCGTCCTTCTGTGGTGGTGGCCGATTCCGGACATTTAACCCAGCTGCGCGACGGCTCCCAGGTCGTCACTCTCAACCAGGGAAcg       <  2:3235974/93‑1 (MQ=255)

                                                                                   gTAATGCGCGTCCTTCTGTGGTGGTGGCCGATTCCGGACATTTAACCCAGCTGCGCGACGGCTCCCAGGTCGTCACTCTCAACCAGGGAAcgc      >  2:5341404/1‑93 (MQ=255)

                                                                                   gTAATGCGCGTCCTTCTGTGGTGGTGGCCGATTCCGGACATTTAACCCAGCTGCGCGACGGCTCCCAGGTCGTCACTCTCAACCAGGGAAcgc      <  2:6125023/93‑1 (MQ=255)

                                                                                   gTAATGCGCGTCCTTCTGTGGTGGTGGCCGATTCCGGACATTTAACCCAGCTGCGCGACGGCTCCCAGGTCGTCACTCTCAACCAGGGAAcgc      <  1:6086271/93‑1 (MQ=255)

                                                                                   gTAATGCGCGTCCTTCTGTGGTGGTGGCCGATTCCGGACATTTAACCCAGCTGCGCGACGGCTCCCAGGTCGTCACTCTCAACCAGGGAAcgc      <  1:5056682/93‑1 (MQ=255)

                                                                                     aaTGCGCGTCCTTCTGTGGTGGTGGCCGATTCCGGACATTTAACCCAGCTGCGCGACGGCTCCCAGGTCGTCACTCTCAACCAGGGAACgcgc    <  1:38922/93‑1 (MQ=255)

                                                                                     aaTGCGCGTCCTTCTGTGGTGGTGGCCGATTCCGGACATTTAACCCAGCTGCGCGACGGCTCCCAGGTCGTCACTCTCAACCAGGGAACgcgc    <  1:2839137/93‑1 (MQ=255)

                                                                                     aaTGCGCGTCCTTCTGTGGTGGTGGCCGATTCCGGACATTTAACCCAGCTGCGCGACGGCTCCCAGGTCGTCACTCTCAACCAGGGAACgcgc    <  2:819860/93‑1 (MQ=255)

                                                                                       tGCGCGTCCTTCTGTGGTGGTGGCCGATTCCGGACATTTAACCCAGCTGCGCGACGGCTCCCAGGTCGTCACTCTCAACCAGGGAACGCGCtt  <  2:5493613/93‑1 (MQ=255)

                                                                                       tGCGCGTCCTTCTGTGGTGGTGGCCGATTCCGGACATTTAACCCAGCTGCGCGACGGCTCCCAGGTCGTCACTCTCAACCAGGGAACGCGCtt  <  2:1362792/93‑1 (MQ=255)

                                                                                       tGCGCGTCCTTCTGTGGTGGTGGCCGATTCCGGACATTTAACCCAGCTGCGCGACGGCTCCCAGGTCGTCACTCTCAACCAGGGAACGCGCtt  <  1:2597630/93‑1 (MQ=255)

TAATGGCAGCTCGGTGCTGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCACGTCCTTCTGTGGTGGTGGCCGATTCCGGACATTTAACCCAGCTGCGCGACGGCTCCCAGGTCGTCACTCTCAACCAGGGAACGCGCTT  >  NC_000913/4484244‑4484423

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

GACTAATGGCAGCTCGGTGCTGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCACGTCCTTCTGTGGTGGTGGCCGATTCCGGACATTTAACCCAGCTGCGCGACGGCTCCCAGGTCGTCACTCTCAACCAGGGAACGCGCTTCGA  >  NC_000913/4484241‑4484426

GACTAATGGCAGCTCGGTGCTGTTCATCGAAAGCGTTGACGGCAGCGCTTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGTTAATGCC                                                                                              >  SRR4302537.4883423/1‑94 (MQ=60)

GACTAATGGCAGCTCGGTGCTGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCG                                                                                              <  SRR4302537.1645557/94‑1 (MQ=60)

 ACTAATGGCAGCTCGGTGCTGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCGC                                                                                             >  SRR4302537.4978608/1‑94 (MQ=60)

 ACTAATGGCAGCTCGGTGCTGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCGC                                                                                             >  SRR4302537.2937136/1‑94 (MQ=60)

  CTAATGGCAGCTCGGTGCTGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCGCG                                                                                            >  SRR4302537.3406570/1‑94 (MQ=60)

  CTAATGGCAGCTCGGTGCTGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCGCG                                                                                            >  SRR4302537.524443/1‑94 (MQ=60)

  CTAATGGCAGCTCGGTGCTGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCGCG                                                                                            >  SRR4302537.5971580/1‑94 (MQ=60)

   TAATGGCAGCTCGGTGCTGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCGCGT                                                                                           >  SRR4302537.4320923/1‑94 (MQ=60)

     ATGGCAGCTCGGTGCTGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCGCGTCC                                                                                         >  SRR4302537.641556/1‑94 (MQ=60)

     ATGGCAGCTCGGTGCTGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCGCGTCC                                                                                         >  SRR4302537.1817834/1‑94 (MQ=60)

      TGGCAGCTCGGTGCTGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCGCGTCCT                                                                                        <  SRR4302537.4261001/94‑1 (MQ=60)

      TGGCAGCTCGGTGCTGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCGCGTCCT                                                                                        <  SRR4302537.5749093/94‑1 (MQ=60)

       GGCAGCTCTGTGCTGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCGCGTCCTT                                                                                       <  SRR4302537.4892065/94‑1 (MQ=60)

        GCAGCTCGGTGCTGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCGCGTCCTTC                                                                                      <  SRR4302537.3753544/94‑1 (MQ=60)

        GCAGCTCGGTGCTGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCGCGTCCTTC                                                                                      <  SRR4302537.1943343/94‑1 (MQ=60)

          AGCTCGGTGCTGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCGCGTCCTTCTG                                                                                    <  SRR4302537.4996731/94‑1 (MQ=60)

           GCTCGGTGCTGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCGCGTCCTTCTGT                                                                                   <  SRR4302537.2591554/94‑1 (MQ=60)

           GCTCGGTGCTGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCGCGTCCTTCTGT                                                                                   <  SRR4302537.396642/94‑1 (MQ=60)

            CTCGGTGCTGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCGCGTCCTTCTGTG                                                                                  >  SRR4302537.4820339/1‑94 (MQ=60)

            CTCGGTGCTGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCGCGTCCTTCTGTG                                                                                  <  SRR4302537.3419839/94‑1 (MQ=60)

               GGTGCTGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCGCGTCCTTCTGTGGTG                                                                               <  SRR4302537.4796084/94‑1 (MQ=60)

                 TGCTGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCGCGTCCTTCTGTGGTGGT                                                                             >  SRR4302537.3087364/1‑94 (MQ=60)

                 TGCTGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCGCGTCCTTCTGTGGTGGT                                                                             >  SRR4302537.5400134/1‑94 (MQ=60)

                 TGCTGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCGCGTCCTTCTGTGGTGGT                                                                             <  SRR4302537.5361739/94‑1 (MQ=60)

                 TGCTGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCGCGTCCTTCTGTGGTGGT                                                                             <  SRR4302537.129787/94‑1 (MQ=60)

                  GCTGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCGCGTCCTTCTGTGGTGGTG                                                                            <  SRR4302537.5217204/94‑1 (MQ=60)

                    TGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCGCGTCCTTCTGTGGTGGTGGC                                                                          >  SRR4302537.6108412/1‑94 (MQ=60)

                    TGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCGCGTCCTTCTGTGGTGGTGGC                                                                          <  SRR4302537.594850/94‑1 (MQ=60)

                    TGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCGCGTCCTTCTGTGGTGGTGGC                                                                          >  SRR4302537.507087/1‑94 (MQ=60)

                     GTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCGCGTCCTTCTGTGGTGGTGGCC                                                                         >  SRR4302537.2898478/1‑94 (MQ=60)

                     GTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCGCGTCCTTCTGTGGTGGTGGCC                                                                         <  SRR4302537.4197570/94‑1 (MQ=60)

                     GTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCGCGTCCTTCTGTGGTGGTGGCC                                                                         >  SRR4302537.146060/1‑94 (MQ=60)

                     GTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCGCGTCCTTCTGTGGTGGTGGCC                                                                         <  SRR4302537.1833797/94‑1 (MQ=60)

                     GTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCGCGTCCTTCTGTGGTGGTGGCC                                                                         >  SRR4302537.4645167/1‑94 (MQ=60)

                      TTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCGCGTCCTTCTGTGGTGGTGGCCG                                                                        >  SRR4302537.4952940/1‑94 (MQ=60)

                      TTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCGCGTCCTTCTGTGGTGGGGGCCG                                                                        >  SRR4302537.4622701/1‑94 (MQ=60)

                       TCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCGCGTCCTTCTGTGGTGGTGGCCGA                                                                       >  SRR4302537.5143446/1‑94 (MQ=60)

                        CATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCGCGTCCTTCTGTGGTGGTGGCCGAT                                                                      <  SRR4302537.6001319/94‑1 (MQ=60)

                         ATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCGCGTCCTTCTGTGGTGGTGGCCGGTT                                                                     >  SRR4302537.5522919/1‑94 (MQ=60)

                         ATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCGCGTCCTTCTGTGGTGGTGGCCGATT                                                                     <  SRR4302537.2860359/94‑1 (MQ=60)

                         ATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCGCGTCCTTCTGTGGTGGTGGCCGATT                                                                     >  SRR4302537.4770728/1‑94 (MQ=60)

                         ATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCGCGTCCTTCTGTGGTGGTGGCCGATT                                                                     <  SRR4302537.706661/94‑1 (MQ=60)

                         ATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCGCGTCCTTCTGTGGTGGTGGCCGATT                                                                     >  SRR4302537.731500/1‑94 (MQ=60)

                           tgctgaaccgctcttccgatctattccgtATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCGCGTCCTTCTGTGGTGGTGGCCGATTCC                                                                   <  SRR4302537.6024832/65‑1 (MQ=60)

                            GAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCGCGTCCTTCTGTGGTGGTGGCCGATTCCG                                                                  >  SRR4302537.3489536/1‑94 (MQ=60)

                             AAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCGCGTCCTTCTGTGGTGGTGGCCGATTCCGG                                                                 >  SRR4302537.2852216/1‑94 (MQ=60)

                               AGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCGCGTCCTTCTGTGGTGGTGGCCGATTCCGGAC                                                               <  SRR4302537.5508986/94‑1 (MQ=60)

                                 CGTTGACGGCAGCGATCTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCGCGTCCTTCTGTGGTGGTGGCCGATTCCGGACAT                                                             >  SRR4302537.4140490/1‑94 (MQ=60)

                                  GTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCGCGTCCTTCTGTGGTGGTGGCCGATTCCGGACATT                                                            >  SRR4302537.2146324/1‑94 (MQ=60)

                                     GACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCGCGTCCTTCTGTGGTGGTGGCCGATTCCGGACATTTAA                                                         <  SRR4302537.2611620/94‑1 (MQ=60)

                                     GACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCGCGTCCTTCTGTGGTGGTGGCCGATTCCGGACATTTAA                                                         <  SRR4302537.1911389/94‑1 (MQ=60)

                                      ACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCGCGTCCTTCTGTGGTGGTGGCCGATTCCGGACATTTAAC                                                        <  SRR4302537.4462251/94‑1 (MQ=60)

                                       CGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCGCGTCCTTCTGTGGTGGTGGCCGATTCCGGACATTTAACC                                                       <  SRR4302537.2740517/94‑1 (MQ=60)

                                       CGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCGCGTCCTTCTGTGGTGGTGGCCGATTCCGGACATTTAACC                                                       <  SRR4302537.5656390/94‑1 (MQ=60)

                                        GGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCGCGTCCTTCTGTGGTGGTGGCCGATTCCGGACATTTAACCC                                                      >  SRR4302537.4894289/1‑94 (MQ=60)

                                        GGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCGCGTCCTTCTGTGGTGGTGGCCGATTCCGGACATTTAACCC                                                      >  SRR4302537.1368118/1‑94 (MQ=60)

                                          CAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGCCCAAAAGGTAATGCGCGTCCTTCTGTGGTGGTGGCCGATTCCGGACATTTAACCCAG                                                    <  SRR4302537.77305/94‑1 (MQ=60)

                                          CAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCGCGTCCTTCTGTGGTGGTGGCCGATTCCGGACATTTAACCCAG                                                    <  SRR4302537.2962123/94‑1 (MQ=60)

                                          CAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCGCGTCCTTCTGTGGTGGTGGCCGATTCCGGACATTTAACCCAG                                                    <  SRR4302537.2860983/94‑1 (MQ=60)

                                           AGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCGCGTCCTTCTGTGGTGGTGGCCGATTCCGGACATTTAACCCAGC                                                   >  SRR4302537.4330018/1‑94 (MQ=60)

                                              GATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCGCGTCCTTCTGTGGTGGTGGCCGATTCCGGACATTTAACCCAGCTGC                                                <  SRR4302537.1065793/94‑1 (MQ=60)

                                              GATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCGCGTCCTTCTGTGGTGGTGGCCGATTCCGGACATTTAACCCAGCTGC                                                >  SRR4302537.4237073/1‑94 (MQ=60)

                                              GATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCGCGTCCTTCTGTGGTGGTGGCCGATTCCGGACATTTAACCCAGCTGC                                                >  SRR4302537.2298387/1‑94 (MQ=60)

                                               ATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCGCGTCCTTCTGTGGTGGTGGCCGATTCCGGACATTTAACCCAGCTGCG                                               >  SRR4302537.951160/1‑94 (MQ=60)

                                               ATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCGCGTCCTTCTGTGGTGGTGGCCGATTCCGGACATTTAACCCAGCTGCG                                               >  SRR4302537.5197327/1‑94 (MQ=60)

                                               ATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCGCGTCCTTCTGTGGTGGTGGCCGATTCCGGACATTTAACCCAGCTGCG                                               >  SRR4302537.5564596/1‑94 (MQ=60)

                                                TTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCGCGTCCTTCTGTGGTGGTGGCCGATTCCGGACATTTAACCCAGCTGCGC                                              <  SRR4302537.1112815/94‑1 (MQ=60)

                                                 TTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCGCGTCCTTCTGTGGTGGTGGCCGATTCCGGACATTTAACCCAGCTGCGCG                                             >  SRR4302537.3017808/1‑94 (MQ=60)

                                                 TTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCGCGTCCTTCTGTGGTGGTGGCCGATTCCGGACATTTAACCCAGCTGCGCG                                             >  SRR4302537.2840863/1‑94 (MQ=60)

                                                  TCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCGCGTCCTTCTGTGGTGGTGGCCGATTCCGGACATTTAACCCAGCTGCGCGC                                            >  SRR4302537.4869345/1‑94 (MQ=60)

                                                  TCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCGCGTCCTTCTGTGGTGGTGGCCGATTCCGGACATTTAACCCAGCTGCGCGA                                            >  SRR4302537.373907/1‑94 (MQ=60)

                                                   CAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCGCGTCCTTCTGTGGTGGTGGCCGATTCCGGACATTTAACCCAGCTGCGCGAC                                           >  SRR4302537.6006699/1‑94 (MQ=60)

                                                   CAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCGCGTCCTTCTGTGGTGGTGGCCGATTCCGGACATTTAACCCAGCTGCGCGAC                                           <  SRR4302537.2766495/94‑1 (MQ=60)

                                                    AAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCGCGTCCTTCTGTGGTGGTGGCCGATTCCGGACATTTAACCCAGCTGCGCGACG                                          >  SRR4302537.1768585/1‑94 (MQ=60)

                                                    AAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCGCGTCCTTCTGTGGTGGTGGCCGATTCCGGACATTTAACCCAGCTGCGCGACG                                          >  SRR4302537.1122134/1‑94 (MQ=60)

                                                    AAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCGCGTCCTTCTGTGGTGGTGGCCGATTCCGGACATTTAACCCAGCTGCGCGACG                                          >  SRR4302537.422730/1‑94 (MQ=60)

                                                     AAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCGCGTCCTTCTGTGGTGGTGGCCGATTCCGGACATTTAACCCAGCTGCGCGACGG                                         >  SRR4302537.1314152/1‑94 (MQ=60)

                                                       GATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCGCGTCCTTCTGTGGTGGTGGCCGATTCCGGACATTTAACCCAGCTGCGCGACGGCT                                       <  SRR4302537.6122246/94‑1 (MQ=60)

                                                       GATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCGCGTCCTTCTGTGGTGGTGGCCGATTCCGGACATTTAACCCAGCTGCGCGACGGCT                                       >  SRR4302537.616967/1‑94 (MQ=60)

                                                        ATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCGCGTCCTTCTGTGGTGGTGGCCGATTCCGGACATTTAACCCAGCTGCGCGACGGCTC                                      >  SRR4302537.312123/1‑94 (MQ=60)

                                                         TGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCGCGTCCTTCTGTGGTGGTGGCCGATTCCGGACATTTAACCCAGCTGCGCGACGGCTCC                                     >  SRR4302537.5601303/1‑94 (MQ=60)

                                                          GTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGAGCGTCCTTCTGTGGTGGTGGCCGATTCCGGACATTTAACCCAGCTGCGCGATGGCTCCC                                    >  SRR4302537.4191021/1‑94 (MQ=60)

                                                           TGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCGCGTCCTTCTGTGGTGGTGGCCGATTCCGGACATTTAACCCAGCTGCGCGACGGCTCCCA                                   >  SRR4302537.1011489/1‑94 (MQ=60)

                                                           TGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCGCGTCCTTCTGTGGTGGTGGCCGATTCCGGACATTTAACCCAGCTGCGCGACGGCTCCCA                                   <  SRR4302537.408600/94‑1 (MQ=60)

                                                            GTTCCTCGCGCAAGTTCGACCAAAAGGTAATGCGCGTCCTTCTGTGGTGGTGGCCGATTCCGGACATTTAACCCAGCTGCGCGACGGCTCCCAG                                  <  SRR4302537.6110489/94‑1 (MQ=60)

                                                            GTTCCTCGCGCAAATTCGACCAAAAGGTAATGCGCGTCCTTCTGTGGTGGTGGCCGATTCCGGACATTTAACCCAGCTGCGCGACGGCTCCCAG                                  <  SRR4302537.3058564/94‑1 (MQ=60)

                                                            GTTCCTCGCGCAAATTCGACCAAAAGGTAATGCGCGTCCTTCTGTGGTGGTGGCCGATTCCGGACATTTAACCCAGCTGCGCGACGGCTCCCAG                                  <  SRR4302537.2381543/94‑1 (MQ=60)

                                                            GTTCCTCGCGCAAATTCGACCAAAAGGTAATGCGCGTCCCTCTGTGGTGGTGGCCGATTCCGGACATTTAACCCAGCTGCGCGACGGCTCCCAG                                  <  SRR4302537.6216213/94‑1 (MQ=60)

                                                              TCCTCGCGCAAATTCGACCAAAAGGTAATGCGCGTCCTTCTGTGGTGGTGGCCGATTCCGGACATTTAACCCAGCTGCGCGACGGCTCCCAGGT                                <  SRR4302537.3266721/94‑1 (MQ=60)

                                                               CCTCGCGCAAATTCGACCAAAAGGTAATGCGCGTCCTTCTGTGGTGGTGGCCGATTCCGGACATTTAACCCAGCTGCGCGACGGCTCCCAGGTC                               >  SRR4302537.5887169/1‑94 (MQ=60)

                                                               CCTCGCGCAAATTCGACCAAAAGGTAATGCGCGTCCTTCTGTGGTGGTGGCCGATTCCGGACATTTAACCCAGCTGCGCGACGGCTCCCAGGTC                               <  SRR4302537.3272258/94‑1 (MQ=60)

                                                                CTCGCGCAAATTCGACCAAAAGGTAATGCGCGTCCTTCTGTGGTGGTGGCCGATTCCGGACATTTAACCCAGCTGCGCGACGGCTCCCAGGCCG                              >  SRR4302537.5252719/1‑94 (MQ=60)

                                                                 TCGCGCAAATTCGACCAAAAGGTAATGCGCGTCCTTCTGTGGTGGTGGCCGATTCCGGACATTTAACCCAGCTGCGCGACGGCTCCCAGGTCGT                             <  SRR4302537.1404179/94‑1 (MQ=60)

                                                                 TCGCGCAAATTCGACCAAAAGGTAATGCGCGTCCTTCTGTGGTGGTGGCCGATTCCGGACATTTAACCCAGCTGCGCGACGGCTCCCAGGTCGT                             >  SRR4302537.4734059/1‑94 (MQ=60)

                                                                   GCGCAAATTCGACCAAAAGGTAATGCGCGTCCTTCTGTGGTGGTGGCCGATTCCGGACATTTAACCCAGCTGCGCGACGGCTCCCAGGTCGTCA                           <  SRR4302537.3433995/94‑1 (MQ=60)

                                                                      CAAATTCGACCAAAAGGTAATGCGCGTCCTTCTGTGGTGGTGGCCGATTCCGGACATTTAACCCAGCTGCGCGACGGCTCCCAGGTCGTCACTC                        >  SRR4302537.4499870/1‑94 (MQ=60)

                                                                       AAATTCGACCAAAAGGTAATGCGCGTCCTTCTGTGGTGGTGGCCGATTCCGGACATTTAACCCAGCTGCGCGACGGCTCCCAGGTCGTCACTCT                       >  SRR4302537.5413150/1‑94 (MQ=60)

                                                                       AAATTCGACCAAAAGGTAATGCGCGTCCTTCTGTGGTGGTGGCCGATTCCGGACATTTAACCCAGCTGCGCGACGGCTCCCAGGTCGTCACTCT                       >  SRR4302537.2958001/1‑94 (MQ=60)

                                                                       AAATTCGACCAAAAGGTAATGCGCGTCCTTCTGTGGTGGTGGCCGATTCCGGACATTTAACCCAGCTGCGCGACGGCTCCCAGGTCGTCACTCT                       <  SRR4302537.1343915/94‑1 (MQ=60)

                                                                        AATTCGACCAAAAGGTAATGCGCGTCCTTCTGTGGTGGTGGCCGATTCCGGACATTTAACCCAGCTGCGCGACGGCTCCCAGGTCGTCACTCTC                      >  SRR4302537.1113840/1‑94 (MQ=60)

                                                                            CGACCAAAAGGTAATGCGCGTCCTTCTGTGGTGGTGGCCGATTCCGGACATTTAACCCAGCTGCGCGACGGCTCCCAGGTCGTCACTCTCAACC                  >  SRR4302537.6330275/1‑94 (MQ=60)

                                                                             GACCAAAAGGTAATGCGCGTCCTTCTGTGGTGGTGGCCGATTCCGGACATTTAACCCAGCTGCGCGACGGCTCCCAGGTCGTCACTCTCAACCA                 <  SRR4302537.6248202/94‑1 (MQ=60)

                                                                             GACCAAAAGGTAATGCGCGTCCTTCTGTGGTGGTGGCCGATTCCGGACATTTAACCCAGCTGCGCGACGGCTCCCAGGTCGTCACTCTCAACCA                 <  SRR4302537.4389394/94‑1 (MQ=60)

                                                                             GACCAAAAGGTAATGCGCGTCCTTCTGTGGTGGTGGCCGATTCCGGACATTTAACCCAGCTGCGCGACGGCTCCCAGGTCGTCACTCTCAACCA                 <  SRR4302537.4167330/94‑1 (MQ=60)

                                                                              ACCAAAAGGTAATGCGCGTCCTTCTGTGGTGGTGGCCGATTCCGGACATTTAACCCAGCTGCGCGACGGCTCCCAGGTCGTCACTCTCAACCAG                >  SRR4302537.4454315/1‑94 (MQ=60)

                                                                               CCAAAAGGTAATGCGCGTCCTTCTGTGGTGGTGGCCGATTCCGGACATTTAACCCAGCTGCGCGACGGCTCCCAGGTCGTCACTCTCAACCAGG               >  SRR4302537.1265625/1‑94 (MQ=60)

                                                                               CCAAAAGGTAATGCGCGTCCTTCTGTGGTGGTGGCCGATTCCGGACATTTAACCCAGCTGCGCGACGGCTCCCAGGTCGTCACTCTCAACCAGG               >  SRR4302537.5980933/1‑94 (MQ=60)

                                                                               CCAAAAGGTAATGCGCGTCCTTCTGTGGTGGTGGCCGATTCCGGACATTTAACCCAGCTGCGCGACGGCTCCCAGGTCGTCACTCTCAACCAGG               >  SRR4302537.117608/1‑94 (MQ=60)

                                                                               CCAAAAGGTAATGCGCGTCCTTCTGTGGTGGTGGCCGATTCCGGACATTTAACCCAGCTGCGCGACGGCTCCCAGGTCGTCACTCTCAACCAGG               >  SRR4302537.5257182/1‑94 (MQ=60)

                                                                                 AAAAGGTAATGCGCGTCCTTCTGTGGTGGTGGCCGATTCCGGACATTTAACCCAGCTGCGCGACGGCTCCCAGGTCGTCACTCTCAACCAGGGA             <  SRR4302537.3990291/94‑1 (MQ=60)

                                                                                  AAAGGTAATGCGCGTCCTTCTGTGGTGGTGGCCGATTCCGGACATTTAACCCAGCTGCGCGACGGCTCCCAGGTCGTCACTCTCAACCAGGGAA            >  SRR4302537.3705674/1‑94 (MQ=60)

                                                                                  AAAGGTAATGCGCGTCCTTCTGGGGTGGTGGCCGATTCCGGACATTTAACCCAGCTGCGCGACGGCTCCCAGGTCGTCACTCTCAACCAGGGAA            >  SRR4302537.6463509/1‑94 (MQ=60)

                                                                                   AAGGTAATGCGCGTCCTTCTGTGGTGGTGGCCGATTCCGGACATTTAACCCAGCTGCGCGACGGCTCCCAGGTCGTCACTCTCAACCAGGGAAC           <  SRR4302537.2938093/94‑1 (MQ=60)

                                                                                    AGGTAATGCGCGTCCTTCTGTGGTGGTGGCCGATTCCGGACATTTAACCCAGCTGCGCGACGGCTCCCAGGTCGTCACTCTCAACCAGGGAACG          <  SRR4302537.2217703/94‑1 (MQ=60)

                                                                                    AGGTAATGCGCGGCCTTCTGTGGTTGTGGCCGATTCCGGACATTTAACCCAGCTGCGCGACGGCTCCCAGGTCGTCACTCTCAACCAGGGAACG          <  SRR4302537.1066987/94‑1 (MQ=60)

                                                                                      GTAATGCGCGTCCTTCTGTGGTGGTGGCCGATTCCGGACATTTAACCCAGCTGCGCGACGGCTCCCAGGTCGTCACTCTCAACCAGGGAACGCG        <  SRR4302537.6128082/94‑1 (MQ=60)

                                                                                      GTAATGCGCGTCCTTCTGTGGTGGTGGCCGATTCCGGACATTTAACCCAGCTGCGCGACGGCTCCCAGGTCGTCACTCTCAACCAGGGAACGCG        <  SRR4302537.5091492/94‑1 (MQ=60)

                                                                                      GTAATGCGCGTCCTTCTGTGGTGGTGGCCGATTCCGGACATTTAACCCAGCTGCGCGACGGCTCCCAGGTCGTCACTCTCAACCAGGGAACGCG        <  SRR4302537.3968256/94‑1 (MQ=60)

                                                                                        AATGCGCGTCCTTCTGTGGTGGTGGCCGATTCCGGACATTTAACCCAGCTGCGCGACGGCTCCCAGGTCGTCACTCTCAACCAGGGAACGCGCT      <  SRR4302537.39218/94‑1 (MQ=60)

                                                                                        AATGCGCGTCCTTCTGTGGTGGTGGCCGATTCCGGACATTTAACCCAGCTGCGCGACGGCTCCCAGGTCGTCACTCTCAACCAGGGAACGCGCT      <  SRR4302537.2859004/94‑1 (MQ=60)

                                                                                          TGCGCGTCCTTCTGTGGTGGTGGCCGATTCCGGACATTTAACCCAGCTGCGCGACGGCTCCCAGGTCGTCACTCTCAACCAGGGAACGCGCTTC    <  SRR4302537.2615801/94‑1 (MQ=60)

                                                                                            CGCGTCCTTCTGTGGTGGTGGCCGATTCCGGACATTTAACCCAGCTGCGCGACGGCTCCCAGGTCGTCACTCTCAACCAGGGAACGCGCTTCGA  >  SRR4302537.2227271/1‑94 (MQ=60)

GACTAATGGCAGCTCGGTGCTGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCACGTCCTTCTGTGGTGGTGGCCGATTCCGGACATTTAACCCAGCTGCGCGACGGCTCCCAGGTCGTCACTCTCAACCAGGGAACGCGCTTCGA  >  NC_000913/4484241‑4484426

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`