Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	882	254.4	12957588	98.0%	12698436	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,500,500	A→G	V167A (GTC→GCC)	yi41 ←	IS4 hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,500,500	0	A	G	100.0%	911.1 / NA	266	V167A (GTC→GCC)	yi41	IS4 hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (127/139); total (127/139)

Only 100 of 271 total aligned reads displayed.

ATAAGTTGCTCAGCAAGCTCATTTTCGCTGTTCTTCATCGTGCCGAAGGCTGCAGCCGTCAGCAGATGGCTGGTCAGTTCCATCTGGCAGACCATTTTGACCTGCGGGTAGAGCGCCGGGTTCCCGGCATGTGTCTGGCGGGGGAAGGCTGCATCGTTCTCTGGTGTATCCGGTGTGCGC  >  NC_000913/4500410‑4500589

 taAGTTGCTCAGCAAGCTCATTTTCGCTGTTCTTCATCGTGCCGAAGGCTGCAGCCGTCAGCAGATGGCTGGTCAGTTCCATCTGGCAGGCCa                                                                                        <  2:2576711/93‑1 (MQ=255)

  aaGTTGCTCAGCAAGCTCATTTTCGCTGTTCTTCATCGTGCCGAAGGCTGCAGCCGTCAGCAGATGGCTGGTCAGTTCCATCTGGCAGGCCAt                                                                                       >  1:4247886/1‑93 (MQ=255)

  aaGTTGCTCAGCAAGCTCATTTTCGCTGTTCTTCATCGTGCCGAAGGCTGCAGCCGTCAGCAGATGGCTGGTCAGTTCCATCTGGCAGGCCAt                                                                                       >  2:707801/1‑93 (MQ=255)

   aGTTGCTCAGCAAGCTCATTTTCGCTGTTCTTCATCGTGCCGAAGGCTGCAGCCGTCAGCAGATGGCTGGTCAGTTCCATCTGGCAGGCCAtt                                                                                      >  1:4292108/1‑93 (MQ=255)

   aGTTGCTCAGCAAGCTCATTTTCGCTGTTCTTCATCGTGCCGAAGGCTGCAGCCGTCAGCAGATGGCTGGTCAGTTCCATCTGGCAGGCCAtt                                                                                      >  1:1548462/1‑93 (MQ=255)

    gTTGCTCAGCAAGCTCATTTTCGCTGTTCTTCATCGTGCCGAAGGCTGCAGCCGTCAGCAGATGGCTGGTCAGTTCCATCTGGCAGGCCAttt                                                                                     <  2:5338272/93‑1 (MQ=255)

     ttGCTCAGCAAGCTCATTTTCGCTGTTCTTCATCGTGCCGAAGGCTGCAGCCGTCAGCAGATGGCTGGTCAGTTCCATCTGGCAGGCCAtttt                                                                                    >  2:2274615/1‑93 (MQ=255)

        cTCAGCAAGCTCATTTTCGCTGTTCTTCATCGTGCCGAAGGCTGCAGCCGTCAGCAGATGGCTGGTCAGTTCCATCTGGCAGGCCATTTTGAc                                                                                 <  2:4415093/93‑1 (MQ=255)

        cTCAGCAAGCTCATTTTCGCTGTTCTTCATCGTGCCGAAGGCTGCAGCCGTCAGCAGATGGCTGGTCAGTTCCATCTGGCAGGCCATTTTGAc                                                                                 >  2:3984134/1‑93 (MQ=255)

         tCAGCAAGCTCATTTTCGCTGTTCTTCATCGTGCCGAAGGCTGCAGCCGTCAGCAGATGGCTGGTCAGTTCCATCTGGCAGGCCATTTTGAcc                                                                                <  1:3456048/93‑1 (MQ=255)

         tCAGCAAGCTCATTTTCGCTGTTCTTCATCGTGCCGAAGGCTGCAGCCGTCAGCAGATGGCTGGTCAGTTCCATCTGGCAGGCCATTTTGAcc                                                                                <  2:558244/93‑1 (MQ=255)

          cAGCAAGCTCATTTTCGCTGTTCTTCATCGTGCCGAAGGCTGCAGCCGTCAGCAGATGGCTGGTCAGTTCCATCTGGCAGGCCATTTTGACCt                                                                               >  1:891544/1‑93 (MQ=255)

           aGCAAGCTCATTTTCGCTGTTCTTCATCGTGCCGAAGGCTGCAGCCGTCAGCAGATGGCTGGTCAGTTCCATCTGGCAGGCCATTTTGACCTg                                                                              <  1:3818072/93‑1 (MQ=255)

           aGCAAGCTCATTTTCGCTGTTCTTCATCGTGCCGAAGGCTGCAGCCGTCAGCAGATGGCTGGTCAGTTCCATCTGGCAGGCCATTTTGACCTg                                                                              <  2:1345820/93‑1 (MQ=255)

            gCAAGCTCATTTTCGCTGTTCTTCATCGTGCCGAAGGCTGCAGCCGTCAGCAGATGGCTGGTCAGTTCCATCTGGCAGGCCATTTTGACCTGc                                                                             >  2:2495208/1‑93 (MQ=255)

            gCAAGCTCATTTTCGCTGTTCTTCATCGTGCCGAAGGCTGCAGCCGTCAGCAGATGGCTGGTCAGTTCCATCTGGCAGGCCATTTTGACCTGc                                                                             >  2:3119773/1‑93 (MQ=255)

             cAAGCTCATTTTCGCTGTTCTTCATCGTGCCGAAGGCTGCAGCCGTCAGCAGATGGCTGGTCAGTTCCATCTGGCAGGCCATTTTGACCTGCg                                                                            <  2:2579450/93‑1 (MQ=255)

                gCTCATTTTCGCTGTTCTTCATCGTGCCGAAGGCTGCAGCCGTCAGCAGATGGCTGGTCAGTTCCATCTGGCAGGCCATTTTGACCTGCGGGt                                                                         <  1:630284/93‑1 (MQ=255)

                 cTCATTTTCGCTGTTCTTCATCGTGCCGAAGGCTGCAGCCGTCAGCAGATGGCTGGTCAGTTCCATCTGGCAGGCCATTTTGACCTGCGGGTa                                                                        <  2:2964618/93‑1 (MQ=255)

                 cTCATTTTCGCTGTTCTTCATCGTGCCGAAGGCTGCAGCCGTCAGCAGATGGCTGGTCAGTTCCATCTGGCAGGCCATTTTGACCTGCGGGTa                                                                        >  1:127638/1‑93 (MQ=255)

                 cTCATTTTCGCTGTTCTTCATCGTGCCGAAGGCTGCAGCCGTCAGCAGATGGCTGGTCAGTTCCATCTGGCAGGCCATTTTGACCTGCGGGTa                                                                        >  2:1492417/1‑93 (MQ=255)

                   cATTTTCGCTGTTCTTCATCGTGCCGAAGGCTGCAGCCGTCAGCAGATGGCTGGTCAGTTCCATCTGGCAGGCCATTTTGACCTGCGGGTaga                                                                      <  2:4992334/93‑1 (MQ=255)

                    aTTTTCGCTGTTCTTCATCGTGCCGAAGGCTGCAGCCGTCAGCAGATGGCTGGTCAGTTCCATCTGGCAGGCCATTTTGACCTGCGGGTagag                                                                     <  1:5546338/93‑1 (MQ=255)

                      tttCGCTGTTCTTCATCGTGCCGAAGGCTGCAGCCGTCAGCAGATGGCTGGTCAGTTCCATCTGGCAGGCCATTTTGACCTGCGGGTagagag                                                                   >  1:1238662/1‑91 (MQ=255)

                      tttCGCTGTTCTTCATCGTGCCGAAGGCTGCAGCCGTCAGCAGATGGCTGGTCAGTTCCATCTGGCAGGCCATTTTGACCTGCGGGTAGAgcg                                                                   >  1:2593642/1‑93 (MQ=255)

                      tttCGCTGTTCTTCATCGTGCCGAAGGCTGCAGCCGTCAGCAGATGGCTGGTCAGTTCCATCTGGCAGGCCATTTTGACCTGCGGGTAGAgcg                                                                   <  2:2057658/93‑1 (MQ=255)

                      tttCGCTGTTCTTCATCGTGCCGAAGGCTGCAGCCGTCAGCAGATGGCTGGTCAGTTCCATCTGGCAGGCCATTTTGACCTGCGGGTAGAgcg                                                                   >  1:1609333/1‑93 (MQ=255)

                        tCGCTGTTCTTCATCGTGCCGAAGGCTGCAGCCGTCAGCAGATGGCTGGTCAGTTCCATCTGGCAGGCCATTTTGACCTGCGGGTAGAGCGcc                                                                 <  2:540582/93‑1 (MQ=255)

                        tCGCTGTTCTTCATCGTGCCGAAGGCTGCAGCCGTCAGCAGATGGCTGGTCAGTTCCATCTGGCAGGCCATTTTGACCTGCGGGTAGAGCGcc                                                                 <  1:5058575/93‑1 (MQ=255)

                         cGCTGTTCTTCATCGTGCCGAAGGCTGCAGCCGTCAGCAGATGGCTGGTCAGTTCCATCTGGCAGGCCATTTTGACCTGCGGGTAGAGCGCCg                                                                <  1:4922970/93‑1 (MQ=255)

                          gCTGTTCTTCATCGTGCCGAAGGCTGCAGCCGTCAGCAGATGGCTGGTCAGTTCCATCTGGCAGGCCATTTTGACCTGCGGGTAGAGCGCCgg                                                               >  1:5452309/1‑93 (MQ=255)

                          gCTGTTCTTCATCGTGCCGAAGGCTGCAGCCGTCAGCAGATGGCTGGTCAGTTCCATCTGGCAGGCCATTTTGACCTGCGGGTAGAGCGCCgg                                                               >  2:399848/1‑93 (MQ=255)

                           cTGTTCTTCATCGTGCCGAAGGCTGCAGCCGTCAGCAGATGGCTGGTCAGTTCCATCTGGCAGGCCATTTTGACCTGCGGGTAGAGCGCCggg                                                              >  1:2980100/1‑93 (MQ=255)

                            tGTTCTTCATCGTGCCGAAGGCTGCAGCCGTCAGCAGATGGCTGGTCAGTTCCATCTGGCAGGCCATTTTGACCTGCGGGTAGAGCGCCGGGt                                                             <  2:5833392/93‑1 (MQ=255)

                             gTTCTTCATCGTGCCGAAGGCTGCAGCCGTCAGCAGATGGCTGGTCAGTTCCATCTGGCAGGCCATTTTGACCTGCGGGTAGAGCGCCGGGtt                                                            >  1:2279169/1‑93 (MQ=255)

                             gTTCTTCATCGTGCCGAAGGCTGCAGCCGTCAGCAGATGGCTGGTCAGTTCCATCTGGCAGGCCATTTTGACCTGCGGGTAGAGCGCCGGGtt                                                            >  1:5252376/1‑93 (MQ=255)

                              ttcttcATCGTGCCGAAGGCTGCAGCCGTCAGCAGATGGCTGGTCAGTTCCATCTGGCAGGCCATTTTGACCTGCGGGTAGAGCGCCGGGTTc                                                           >  2:5259252/1‑93 (MQ=255)

                              ttcttcATCGTGCCGAAGGCTGCAGCCGTCAGCAGATGGCTGGTCAGTTCCATCTGGCAGGCCATTTTGACCTGCGGGTAGAGCGCCGGGTTc                                                           >  2:677272/1‑93 (MQ=255)

                              ttcttcATCGTGCCGAAGGCTGCAGCCGTCAGCAGATGGCTGGTCAGTTCCATCTGGCAGGCCATTTTGACCTGCGGGTAGAGCGCCGGGTTc                                                           >  2:1412455/1‑93 (MQ=255)

                               tcttcATCGTGCCGAAGGCTGCAGCCGTCAGCAGATGGCTGGTCAGTTCCATCTGGCAGGCCATTTTGACCTGCGGGTAGAGCGCCGGGTTcc                                                          <  1:6457878/93‑1 (MQ=255)

                               tcttcATCGTGCCGAAGGCTGCAGCCGTCAGCAGATGGCTGGTCAGTTCCATCTGGCAGGCCATTTTGACCTGCGGGTAGAGCGCCGGGTTcc                                                          >  2:760317/1‑93 (MQ=255)

                                cttcATCGTGCCGAAGGCTGCAGCCGTCAGCAGATGGCTGGTCAGTTCCATCTGGCAGGCCATTTTGACCTGCGGGTAGAGCGCCGGGTTccc                                                         <  2:2336596/93‑1 (MQ=255)

                                     tCGTGCCGAAGGCTGCAGCCGTCAGCAGATGGCTGGTCAGTTCCATCTGGCAGGCCATTTTGACCTGCGGGTAGAGCGCCGGGTTGCCGGCAt                                                    >  2:3231806/1‑93 (MQ=255)

                                     tCGTGCCGAAGGCTGCAGCCGTCAGCAGATGGCTGGTCAGTTCCATCTGGCAGGCCATTTTGACCTGCGGGTAGAGCGCCGGGTTCCCGGCAt                                                    <  1:6254478/93‑1 (MQ=255)

                                      cGTGCCGAAGGCTGCAGCCGTCAGCAGATGGCTGGTCAGTTCCATCTGGCAGGCCATTTTGACCTGCGGGTAGAGCGCCGGGTTCCCGGCAtg                                                   <  2:608797/93‑1 (MQ=255)

                                       gTGCCGAAGTCTGCAGCCGTCAGCAGATGGCTGGTCAGTTCCATCTGGCAGGCCATTTTGACCTGCGGGTAGAGCGCCGGGTTCCCGGCAtgt                                                  <  2:5943047/93‑1 (MQ=255)

                                        tGCCGAAGGCTGCAGCCGTCAGCAGATGGCTGGTCAGTTCCATCTGGCAGGCCATTTTGACCTGCGGGTAGAGCGCCGGGTTCCCGGCAtgtg                                                 <  2:842877/93‑1 (MQ=255)

                                        tGCCGAAGGCTGCAGCCGTCAGCAGATGGCTGGTCAGTTCCATCTGGCAGGCCATTTTGACCTGCGGGTAGAGCGCCGGGTTCCCGGCAtgtg                                                 >  1:206187/1‑93 (MQ=255)

                                         gCCGAAGGCGGCAGCCGTCAGCAGATGGCTGGTCAGTTCCATCTGGCAGGCCATTTTGACCTGCGGGTAGAGCGCCGGGTTCCCGGCATgtgt                                                <  1:1201262/93‑1 (MQ=255)

                                          ccGAAGGCTGCAGCCGTCAGCAGATGGCTGGTCAGTTCCATCTGGCAGGCCATTTTGACCTGCGGGTAGAGCGCCGGGTTCCCGGCATGTGTc                                               <  2:4601770/93‑1 (MQ=255)

                                           cGAAGGCTGCAGCCGTCAGCAGATGGCTGGTCAGTTCCATCTGGCAGGCCATGTTGACCTGCGGGTAGAGCGCCGGGTTCCCGGCATGTGTCt                                              <  1:3030091/93‑1 (MQ=255)

                                            gAAGGCTGCAGCCGTCAGCAGATGGCTGTTCAGTTCCATCTGGCAGGCCATTTTGACCTGCGGGTAGAGCGCCGGGTTCCCGGCATGTGTCTg                                             <  2:1350250/93‑1 (MQ=255)

                                            gAAGGCTGCAGCCGTCAGCAGATGGCTGGTCAGTTCCATCTGGCAGGCCATTTTGACCTGCGGGTAGAGCGCCGGGTTCCTGGCATGTGTCTg                                             >  1:1040183/1‑93 (MQ=255)

                                             aaGGCTGCAGCCGTCAGCAGATGGCTGGTCAGTTCCATCTGGCAGGCCATTTTGACCTGCGGGTAGAGCGCCGGGTTCCCGGCATGTGTCTgg                                            <  2:1655116/93‑1 (MQ=255)

                                              aGGCTGCAGCCGTCAGCAGATGGCTGGTCAGTTCCATCTGGCAGGCCATTTTGACCTGCGGGTAGAGCGCCGGGTTCCCGGCATGTGTCTGGc                                           >  1:5602616/1‑93 (MQ=255)

                                              aGGCTGCAGCCGTCAGCAGATGGCTGGTCAGTTCCATCTGGCAGGCCATTTTGACCTGCGGGTAGAGCGCCGGGTTCCCGGCATGTGTCTGGc                                           <  2:2528348/93‑1 (MQ=255)

                                               ggCTGCAGCCGTCAGCAGATGGCTGGTCAGTTCCATCTGGCAGGCCATTTTGACCTGCGGGTAGAGCGCCGGGGTCCCGGCATGTGTGTGGCg                                          >  1:5737600/1‑93 (MQ=255)

                                                gCTGCAGCCGTCAGCAGATGGCTGGTCAGTTCCATCTGGCAGGCCATTTTGACCTGCGGGTAGAGCGCCGGGTTCCAGGCATGTGTCTGGCgg                                         <  2:3464547/93‑1 (MQ=255)

                                                 cTGCAGCCGTCAGCAGATGGCTGGTCAGTTCCATCTGGCAGGCCATTTTGACCTGCGGGTAGAGCGCCGGGTTCCCGGCATGTGTCTGGCggg                                        >  2:4366275/1‑93 (MQ=255)

                                                 cTGCAGCCGTCAGCAGATGGCTGGTCAGTTCCATCTGGCAGGCCATTTTGACCTGCGGGTAGAGCGCCGGGTTCCCGGCATGTGTCTGGCggg                                        >  1:3497460/1‑93 (MQ=255)

                                                  tGCAGCCGTCAGCAGATGGCTGGTCAGTTCCATCTGGCAGGCCATTTTGACCTGCGGGTAGAGCGCCGGGTTCCCGGCATGTGTCTGGCgggg                                       >  1:191855/1‑93 (MQ=255)

                                                      gCCGTCAGCAGATGGCTGGTCAGTTCCATCTGGCAGGCCATTTTGACCTGCGGGTAGAGCGCCGGGTTCCCGGCATGTGTCTGGCGGGGGAAg                                   <  2:2172567/93‑1 (MQ=255)

                                                       ccGTCAGCAGATGGCTGGTCAGTTCCATCTGGCAGGCCATTTTGACCTGCGGGTAGAGCGCCGGGTTCCCGGCATGTGTCTGGCGGGGGAAgg                                  <  2:2937499/93‑1 (MQ=255)

                                                        cGTCAGCAGATGGCTGGTCAGTTCCATCTGGCAGGCCATTTTGACCTGCGGGTAGAGCGCCGGGTTCCCGGCATGTGTCTGGCGGGGGAAGGc                                 <  2:4783351/93‑1 (MQ=255)

                                                        cGTCAGCAGATGGCTGGTCAGTTCCATCTGGCAGGCCATTTTGACCTGCGGGTAGAGCGCCGGGTTCCCGGCATGTGTCTGGCGGGGGAAGGc                                 >  2:3645138/1‑93 (MQ=255)

                                                           cagcagATGGCTGGTCAGTTCCATCTGGCAGGCCATTTTGACCTGCGGGTAGAGCGCCGGGTTCCCGGCATGTGTCTGGCGGGGGAAGGCTGc                              <  1:2773811/93‑1 (MQ=255)

                                                            agcagATGGCTGGTCAGTTCCATCTGGCAGGCCATTTTGACCTGCGGGTAGAGCGCCGGGTTCCCGGCATGTGTCTGGCGGGGGAAGGCTGCa                             >  1:2931686/1‑93 (MQ=255)

                                                             gcagATGGCTGGTCAGTTCCATCTGGCAGGCCATTTTGACCTGCGGGTAGAGCGCCGGGTTCCCGGCATGTGTCTGGCGGGGGAAGGCTGCAt                            >  2:543436/1‑93 (MQ=255)

                                                                  tGGCTGGTCAGTTCCATCTGGCAGGCCATTTTGACCTGCGGGTAGAGGGCCGGGTTCCCGGCATGTGGCTGGCGGGGGAAGGGTGCATCGTtc                       >  1:4610644/1‑93 (MQ=255)

                                                                  tGGCTGGTCAGTTCCATCTGGCAGGCCATTTTGACCTGCGGGTAGAGCGCCGGGTTCCCGGCATGTGTCTGGCGGGGGAAGGCTGCATCGTtc                       <  2:3906106/93‑1 (MQ=255)

                                                                  tGGCTGGTCAGTTCCATCTGGCAGGCCATTTTGACCTGCGGGTAGAGCGCCGGGTTCCCGGCATGTGTCTGGCGGGGGAAGGCTGCATCGTtc                       >  1:2202661/1‑93 (MQ=255)

                                                                  tGGCTGGTCAGTTCCATCTGGCAGGCCATTTTGACCTGCGGGTAGAGCGCCGGGTTCCCGGCATGTGTCTGGCGGGGGAAGGCTGCATCGTtc                       >  1:775104/1‑93 (MQ=255)

                                                                   ggCTGGTCAGTTCCATCTGGCAGGCCATTTTGACCTGCGGGTAGAGCGCCGGGTTCCCGGCATGTGTCTGGCGGGGGACGGCTGCATCATtct                      >  2:2875164/1‑93 (MQ=255)

                                                                    gCTGGTCAGTTCCATCTGGCAGGCCATTTTGACCTGCGGGTAGAGCGCCGGGTTCCCGGCATGTGTCTGGCGGGGGAAGGCTGCATCGTtctc                     <  2:1045483/93‑1 (MQ=255)

                                                                     cTGGTCAGTTCCATCTGGCAGGCCATTTTGACCTGCGGGTAGAGCGCCGGGTTCCCGGCATGTGTCTGGCGGGGGAAGGCTGCATCGTTctct                    <  1:5765137/93‑1 (MQ=255)

                                                                        gTCAGTTCCATCTGGCAGGCCATTTTGACCTGCGGGTAGAGCGCCGGGTTCCCGGCATGTGTCTGGCGGGGGAAGGCTGCATCGTTCTCTGgt                 <  2:1882006/93‑1 (MQ=255)

                                                                        gTCAGTTCCATCTGGCAGGCCATTTTGACCTGCGGGTAGAGCGCCGGGTTCCCGGCATGTGTCTGGCGGGGGAAGGCTGCATCGTTCTCTGgt                 >  1:790765/1‑93 (MQ=255)

                                                                        gTCAGTTCCATCTGGCAGGCCATTTTGACCTGCGGGTAGAGCGCCGGGTTCCCGGCATGTGTCTGGCGGGGGAAGGCTGCATCGTTCTCTGgt                 <  2:4457635/93‑1 (MQ=255)

                                                                          cAGTTCCATCTGGCAGGCCATTTTGACCTGCGGGTAGAGCGCCGGGTTCCCGGCATGTGTCTGGCGGGGGAAGGCTGCATCGTTCTCTGgtgt               >  1:4859789/1‑93 (MQ=255)

                                                                          cAGTTCCATCTGGCAGGCCATTTTGACCTGCGGGTAGAGCGCCGGGTTCCCGGCATGTGTCTGGCGGGGGAAGGCTGCATCGTTCTCTGgtgt               >  2:6340245/1‑93 (MQ=255)

                                                                           aGTTCCATTTGGCAGGCCATTTTGACCTGCGGGTAGAGCGCCGGGTTCCCGGCATGTGTCTGGCGGGGGAAGGCTGCATCGTTCTCTGGTGTa              >  2:6282711/1‑93 (MQ=255)

                                                                           aGTTCCATCTGGCAGGCCATTTTGACCTGCGGGTAGAGCGCCGGGTTCCCGGCATGTGTCTGGCTGGGGAAGGCTGCATCGTTCTCTGGTGTa              <  2:1774135/93‑1 (MQ=255)

                                                                           aGTTCCATCTGGCAGGCCATTTTGACCTGCGGGTAGAGCGCCGGGTTCCCGGCATGTGTCTGGCGGGGGAAGGCTGCATCGTTCTCTGGTGTa              <  1:5977455/93‑1 (MQ=255)

                                                                           aGTTCCATCTGGCAGGCCATTTTGACCTGCGGGTAGAGCGCCGGGTTCCCGGCATGTGTCTGGCGGGGGAAGGCTGCATCGTTCTCTGGTGTa              <  1:174956/93‑1 (MQ=255)

                                                                            gTTCCATCTGGCAGGCCATTTTGACCTGCGGGTAGAGCGCCGGGTTCCCGGCATGTGTCTGGCGGGGGAAGGCTGCATCGTTCTCTGGTGTAt             >  1:4162126/1‑93 (MQ=255)

                                                                             ttCCATCTGTCAGGCCATTTTGACCTGCGGGTAGAGCGCCGGGTTCCCGGCATGTGTCTGGCGGGGGAAGGCTGCATCGTTCTCTGGTGTATc            <  1:3126079/93‑1 (MQ=255)

                                                                             ttCCATCTGGCAGGCCATTTTGACCTGCGGGTAGAGCGCCGGGTTCCCGGCATGTGTCTGGCGGGGGACGGCTGCATCATTCTCTGGTGTATc            >  1:1358097/1‑93 (MQ=255)

                                                                             ttCCATCTGGCAGGCCATTTTGACCTGCGGGTAGAGCGCCGGGTTCCCGGCATGTGTCTGGCGGGGGAAGGCTGCATCGTTCTCTGGTGTATc            <  1:217194/93‑1 (MQ=255)

                                                                             ttCCATCTGGCAGGCCATTTTGACCTGCGGGTAGAGCGCCGGGTTCCCGGCATGTGTCTGGCGGGGGAAGGCTGCATCGTTCTCTGGTGTATc            <  2:5172271/93‑1 (MQ=255)

                                                                             ttCCATCTGGCAGGCCATTTTGACCTGCGGGTAGAGCGCCGGGTTCCCGGCATGTGTCTGGCGGGGGAAGGCTGCATCGTTCTCTGGTGTATc            >  2:3829116/1‑93 (MQ=255)

                                                                               ccATCTGGCAGGCCATTTTGACCTGCGGGTAGAGCGCCGGGTTCCCGGCATGTGTCTGGCGGGGGAAGGCTGCATCGTTCTCTGGTGTATCCg          >  1:3257070/1‑93 (MQ=255)

                                                                                 aTCTGGCAGGCCATTTTGACCTGCGGGTAGAGCGCCGGGTTCCCGGCATGTGTCTGGCGGGGGAAGGCTGCATCGTTCTCTGGTGTATCCGgt        >  1:109321/1‑93 (MQ=255)

                                                                                 aTCTGGCAGGCCATTTTGACCTGCGGGTAGAGCGCCGGGTTCCCGGCATGTGTCTGGCGGGGGAAGGCTGCATCGTTCTCTGGTGTATCCGgt        <  1:4891575/93‑1 (MQ=255)

                                                                                   cTGGCAGGCCATTTTGACCTGCGGGTAGAGCGCCGGGTTCCCGGCATGTGTCTGGCGGGGGAAGGCTGCATCGTTCTCTGGTGTATCCGgtgt      <  2:4122556/93‑1 (MQ=255)

                                                                                    tGGCAGGCCATTTTGACCTGCGGGTAGAGCGCCGGGTTCCCGGCATGTGTCTGGCGGGGGAAGGCTGCATCGTTCTCTGGTGTATCCGGtgtg     <  1:455950/93‑1 (MQ=255)

                                                                                     ggCAGGCCATTTTGACCTGCGGGTAGAGCGCCGGGTTCCCGGCATGTGTCTGGCGGGGGAAGGCTGCATCGTTCTCTGGTGTATCCGGTGTgc    <  1:2891586/93‑1 (MQ=255)

                                                                                      gCAGGCCATTTTGACCTGCGGGTAGAGCGCCGGGTTCCCGGCATGTGTCTGGCGGGGGAAGGCTGCATCGTTCTCTGGTGTATCCGGTGTgcg   >  1:2372975/1‑93 (MQ=255)

                                                                                      gCAGGCCATTTTGACCTGCGGGTAGAGCGCCGGGTTCCCGGCATGTGTCTGGCGGGGGAAGGCTGCATCGTTCTCTGGTGTATCCGGTGTgcg   <  1:370610/93‑1 (MQ=255)

                                                                                       cAGGCCATTTTGACCTGCGGGTAGAGCGCCGGGTTCCCGGCATGTGTCTGGCGGGGGAAGGCTGCGTCGTTCTCTGGTGTATCCGGTGTgcgc  >  1:1679690/1‑93 (MQ=255)

                                                                                       cAGGCCATTTTGACCTGCGGGTAGAGCCCCCGGTTCCCGGCATGTGTCTGGCGGGGCGCGGCTGCATCGTTCTCTGGCGTATCCCgcgcgcgc  >  2:2390035/1‑93 (MQ=255)

ATAAGTTGCTCAGCAAGCTCATTTTCGCTGTTCTTCATCGTGCCGAAGGCTGCAGCCGTCAGCAGATGGCTGGTCAGTTCCATCTGGCAGACCATTTTGACCTGCGGGTAGAGCGCCGGGTTCCCGGCATGTGTCTGGCGGGGGAAGGCTGCATCGTTCTCTGGTGTATCCGGTGTGCGC  >  NC_000913/4500410‑4500589

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

TCTATAAGTTGCTCAGCAAGCTCATTTTCGCTGTTCTTCATCGTGCCGAAGGCTGCAGCCGTCAGCAGATGGCTGGTCAGTTCCATCTGGCAGACCATTTTGACCTGCGGGTAGAGCGCCGGGTTCCCGGCATGTGTCTGGCGGGGGAAGGCTGCATCGTTCTCTGGTGTATCCGGTGTGCGCCAG  >  NC_000913/4500407‑4500592

TCTATAAGTTGCTCAGCAAGCTCATTTTCGCTGTTCTTCATCGTGCCGAAGGCTGCAGCCGTCAGCAGATGGCTGGTCAGTTCCATCTGGCAGG                                                                                              >  SRR4302537.6325850/1‑94 (MQ=60)

 CTATAAGTTGCTCAGCAAGCTCATTTTCGCTGTTCTTCATCGTGCCGAAGGCTGCAGCCGTCAGCAGATGGCTGGTCAGTTCCATCTGGCAGGC                                                                                             >  SRR4302537.1755252/1‑94 (MQ=60)

 CTATAAGTTGCTCAGCAAGCTCATTTTCGCTGTTCTTCATCGTGCCGAAGGCTGCAGCCGTCAGCAGATGGCTGGTCAGTTCCATCTGGCAGGC                                                                                             <  SRR4302537.3032234/94‑1 (MQ=60)

   ATAAGTTGCTCAGCAAGCTCATTTTCGCTGTTCTTCATCGTGCCGAAGGCTGCAGCCGTCAGCAGATGGCTGGTCAGTTCCATCTGGCAGGCCA                                                                                           >  SRR4302537.6399369/1‑94 (MQ=60)

    TAAGTTGCTCAGCAAGCTCATTTTCGCTGTTCTTCATCGTGCCGAAGGCTGCAGCCGTCAGCAGATGGCTGGTCAGTTCCATCTGGCAGGCCAT                                                                                          >  SRR4302537.4277406/1‑94 (MQ=60)

     AAGTTGCTCAGCAAGCTCATTTTCGCTGTTCTTCATCGTGCCGAAGGCTGCAGCCGTCAGCAGATGGCTGGTCAGTTCCATCTGGCAGGCCATT                                                                                         >  SRR4302537.4321937/1‑94 (MQ=60)

     AAGTTGCTCAGCAAGCTCATTTTCGCTGTTCTTCATCGTGCCGAAGGCTGCAGCCGTCAGCAGATGGCTGGTCAGTTCCATCTGGCAGGCCATT                                                                                         <  SRR4302537.1914038/94‑1 (MQ=60)

     AAGTTGCTCAGCAAGCTCATTTTCGCTGTTCTTCATCGTGCCGAAGGCTGCAGCCGTCAGCAGATGGCTGGTCAGTTCCATCTGGCAGGCCATT                                                                                         >  SRR4302537.1559269/1‑94 (MQ=60)

      AGTTGCTCAGCAAGCTCATTTTCGCTGTTCTTCATCGTGCCGAAGGCTGCAGCCGTCAGCAGATGGCTGGTCAGTTCCATCTGGCAGGCCATTT                                                                                        <  SRR4302537.794406/94‑1 (MQ=60)

          GCTCAGCAAGCTCATTTTCGCTGTTCTTCATCGTGCCGAAGGCTGCAGCCGTCAGCAGATGGCTGGTCAGTTCCATCTGGCAGGCCATTTTGAC                                                                                    >  SRR4302537.217126/1‑94 (MQ=60)

           CTCAGCAAGCTCATTTTCGCTGTTCTTCATCGTGCCGAAGGCTGCAGCCGTCAGCAGATGGCTGGTCAGTTCCATCTGGCAGGCCATTTTGACC                                                                                   >  SRR4302537.4822061/1‑94 (MQ=60)

           CTCAGCAAGCTCATTTTCGCTGTTCTTCATCGTGCCGAAGGCTGCAGCCGTCAGCAGATGGCTGGTCAGTTCCATCTGGCAGGCCATTTTGACC                                                                                   <  SRR4302537.4904098/94‑1 (MQ=60)

           CTCAGCAAGCTCATTTTCGCTGTTCTTCATCGTGCCGAAGGCTGCAGCCGTCAGCAGATGGCTGGTCAGTTCCATCTGGAAGGCCATTTTGACC                                                                                   >  SRR4302537.4270966/1‑94 (MQ=60)

            TCAGCAAGCTCATTTTCGCTGTTCTTCATCGTGCCGAAGGCTGCAGCCGTCAGCAGATGGCTGGTCAGTTCCATCTGGCAGGCCATTTTGACCT                                                                                  >  SRR4302537.897687/1‑94 (MQ=60)

            TCAGCAAGCTCATTTTCGCTGTTCTTCATCGTGCCGAAGGCTGCAGCCGTCAGCAGATGGCTGGTCAGTTCCATCTGGCAGGCCATTTTGACCT                                                                                  <  SRR4302537.3480486/94‑1 (MQ=60)

             CAGCAAGCTCATTTTCGCTGTTCTTCATCGTGCCGAAGGCTGCAGCCGTCAGCAGATGGCTGGTCAGTTCCATCTGGCAGGCCATTTTGACCTG                                                                                 >  SRR4302537.5820957/1‑94 (MQ=60)

              AGCAAGCTCATTTTCGCTGTTCTTCATCGTGCCGAAGGCTGCAGCCGTCAGCAGATGGCTGGTCAGTTCCATCTGGCAGGCCATTTTGACCTGC                                                                                <  SRR4302537.3844845/94‑1 (MQ=60)

                   GCTCATTTTCGCTGTTCTTCATCGTGCCGAAGGCTGCAGCCGTCAGCAGATGGCTGGTCAGTTCCATCTGGCAGGCCATTTTGACCTGCGGGTA                                                                           <  SRR4302537.634568/94‑1 (MQ=60)

                   GCTCATTTTCGCTGTTCTTCATCGTGCCGAAGGCTGCAGCCGTCAGCAGATGGCTGGTCAGTTCCATCTGGCAGGCCATTTTGACCTGCGGGTA                                                                           >  SRR4302537.128551/1‑94 (MQ=60)

                   ACTCATTTTCGCTGTTCTTCATCGTGCCGAAGGCTGCATCCGTCAGCAGATGGCTGGTCAGTTCCATCTGGCAGGCCATTTTGACCTGCGGGTA                                                                           <  SRR4302537.5752402/94‑1 (MQ=60)

                    CTCATTTTCGCTGTTCTTCATCGTGCCGAAGGCTGCAGCCGTCAGCAGATGGCTGGTCAGTTCCATCTGGCAGGCCATTTTGACCTGCGGGTAG                                                                          >  SRR4302537.4211404/1‑94 (MQ=60)

                    CTCATTTTCGCTGTTCTTCATCGTGCCGAAGGCTGCAGCCGTCAGCAGATGGCTGGTCAGTTCCATCTGGCAGGCCATTTTGACCTGCGGGTAG                                                                          <  SRR4302537.3921489/94‑1 (MQ=60)

                    CTCATTTTCGCTGTTCTTCATCGTGCCGAAGGCTGCAGCCGTCAGCAGATGGCTGGTCAGTTCCATCTGGCAGGCCATTTTGACCTGCGGGTAG                                                                          >  SRR4302537.220214/1‑94 (MQ=60)

                    CTCATTTTCGCTGTTCTTCATCGTGCCGAAGGCTGCAGCCGTCAGCAGATGGCTGGTCAGTTCCATCTGGCAGGCCATTTTGACCTGCGGGTAG                                                                          <  SRR4302537.1240159/94‑1 (MQ=60)

                     TCATTTTCGCTGTTCTTCATCGTGCCGAAGGCTGCAGCCGTCAGCAGATGGCTGGTCAGTTCCATCTGGCAGGCCATTTTGACCTGCGGGTAGA                                                                         >  SRR4302537.3192728/1‑94 (MQ=60)

                     TCATTTTCGCTGTTCTTCATCGTGCCGAAGGCTGCAGCCGTCAGCAGATGGCTGGTCAGTTCCATCTGGCAGGCCATTTTGACCTGCGGGGAGA                                                                         >  SRR4302537.4605824/1‑94 (MQ=60)

                      CATTTTCGCTGTTCTTCATCGTGCCGAAGGCTGCAGCCGTCAGCAGATGGCTGGTCAGTTCCATCTGGCAGGCCATTTTGACCTGCGGGTAGAG                                                                        <  SRR4302537.1710199/94‑1 (MQ=60)

                      CATTTTCGCTGTTCTTCATCGTGCCGAAGGCTGCAGCCGTCAGCAGATGGCTGGTCAGTTCCATCTGGCAGGCCATTTTGACCTGCGGGTAGAG                                                                        >  SRR4302537.5236304/1‑94 (MQ=60)

                       ATTTTCGCTGTTCTTCATCGTGCCGAAGGCTGCAGCCGTCAGCAGATGGCTGGTCAGTTCCATCTGGCAGGCCATTTTGCCCTGCGGGGAGAGC                                                                       >  SRR4302537.3164352/1‑94 (MQ=60)

                       ATTTTCGCTGTTCTTCATCGTGCCGAAGGCTGCAGCCGTCAGCAGATGGCTGGTCAGTTCCATCTGGCAGGCCATTTTGACCTGCGGGTAGAGC                                                                       <  SRR4302537.5584210/94‑1 (MQ=60)

                       ATTTTCGCTGTTCTTCATCGTGCCGAAGGCTGCAGCCGTCAGCAGATGGCTGGTCAGTTCCATCTGGCAGGCCATTTTGACCTGCGGGTAGAGC                                                                       >  SRR4302537.803728/1‑94 (MQ=60)

                        TTTTCGCTGTTCTTCATCGTGCCGAAGGCTGCAGCCGTCAGCAGATGGCTGGTCAGTTCCATCTGGCAGGCCATTTTGACCTGCGGGTAGAGCG                                                                      >  SRR4302537.1620584/1‑94 (MQ=60)

                        TTTTCGCTGTTCTTCATCGTGCCGAAGGCTGCAGCCGTCAGCAGATGGCTGGTCAGTTCCATCTGGCAGGCCATTTTGACCTGCGGGTAGAGCG                                                                      >  SRR4302537.2611785/1‑94 (MQ=60)

                        TTTTCGCTGTTCTTCATCGTGCCGAAGGCTGCAGCCGTCAGCAGATGGCTGGTCAGTTCCATCTGGCAGGCCATTTTGACCTGCGGGTAGAGCG                                                                      <  SRR4302537.337063/94‑1 (MQ=60)

                        TTTTCGCTGTTCTTCATCGTGCCGAAGGCTGCAGCCGTCAGCAGATGGCTGGTCAGTTCCATCTGGCAGGCCATTTTGACCTGCGGGTAGAGCG                                                                      >  SRR4302537.313628/1‑94 (MQ=60)

                        TTTTCGCTGTTCTTCATCGTGCCGAAGGCTGCAGCCGTCAGCAGATGGCTGGTCAGTTCCATCTGGCAGGCCATTTTGACCTGCGGGTAGAGAG                                                                      >  SRR4302537.1247277/1‑94 (MQ=60)

                           TCGCTGTTCTTCATCGTGCCGAAGGCTGCAGCCGTCAGCAGATGGCTGGTCAGTTCCATCTGGCAGGCCATTTTGACCTGCGGGTAGAGCGCCG                                                                   <  SRR4302537.5093396/94‑1 (MQ=60)

                           TCGCTGTTCTTCATCGTGCCGAAGGCTGCAGCCGTCAGCAGATGGCTGGTCAGTTCCATCTGGCAGGCCATTTTGACCTGCGGGTAGAGCGCCG                                                                   <  SRR4302537.1481535/94‑1 (MQ=60)

                            CGCTGTTCTTCATCGTGCCGAAGGCTGCAGCCGTCAGCAGATGGCTGGTCAGTTCCATCTGGCAGGCCATTTTGACCTGCGGGTAGAGCGCCGG                                                                  <  SRR4302537.6134281/94‑1 (MQ=60)

                            CGCTGTTCTTCATCGTGCCGAAGGCTGCAGCCGTCAGCAGATGGCTGGTCAGTTCCATCTGGCAGGCCATTTTGACCTGCGGGTAGAGCGCCGG                                                                  <  SRR4302537.4956921/94‑1 (MQ=60)

                            CGCTGTTCTTCATCGTGCCGAAGGCTGCAGCCGTCAGCAGATGGCTGGTCAGTTCCATCTGGCAGGCCATTTTGACCTGCGGGTAGAGCGCCGG                                                                  >  SRR4302537.5489498/1‑94 (MQ=60)

                             GCTGTTCTTCATCGTGCCGAAGGCTGCAGCCGTCAGCAGATGGCTGGTCAGTTCCATCTGGCAGGCCATTTTGACCTGCGGGTAGAGCGCCGGG                                                                 <  SRR4302537.4301372/94‑1 (MQ=60)

                             GCTGTTCTTCATCGTGCCGAAGGCTGCAGCCGTCAGCAGATGGCTGGTCAGTTCCATCTGGCAGGCCATTTTGACCTGCGGGTAGAGCGCCGGG                                                                 >  SRR4302537.3000948/1‑94 (MQ=60)

                              CTGTTCTTCATCGTGCCGAAGGCTGCAGCCGTCAGCAGATGGCTGGTCAGTTCCATCTGGCAGGCCATTTTGACCTGCGGGTAGAGCGCCGGGT                                                                >  SRR4302537.4286592/1‑94 (MQ=60)

                               TGTTCTTCATCGTGCCGAAGGCTGCAGCCGTCAGCAGATGGCTGGTCAGTTCCATCTGGCAGGCCATTTTGACCTGCGGGTAGAGCGCCGGGTT                                                               >  SRR4302537.5288517/1‑94 (MQ=60)

                               TGTTCTTCATCGTGCCGAAGGCTGCAGCCGTCAGCAGATGGCTGGTCAGTTCCATCTGGCAGGCCATTTTGACCTGCGGGTAGAGCGCCGGGTT                                                               >  SRR4302537.2295259/1‑94 (MQ=60)

                               TGTTCTTCATCGTGCCGAAGGCTGCAGCCGTCAGCAGATGGCTGGTCAGTTCCATCTGGCAGGCCATTTTGACCTGCGGGTAGAGCGCCGGGTT                                                               <  SRR4302537.2948928/94‑1 (MQ=60)

                                GTTCTTCATCGTGCCGAAGGCTGCAGCCGTCAGCAGATGGCTGGTCAGTTCCATCTGGCAGGCCATTTTGACCTGCGGGTAGAGCGCAGGGTTC                                                              >  SRR4302537.1232659/1‑94 (MQ=60)

                                GTTCTTCATCGTGCCGAAGGCTGCAGCCGTCAGCAGATGGCTGCTCAGTTCCATCTGGCAGGCCATTTTGACCTGCGGGTAGAGCGCCGGGTTC                                                              >  SRR4302537.3481380/1‑94 (MQ=60)

                                 TTCTTCATCGTGCCGAAGGCTGCAGCCGTCAGCAGATGGCTGGTCAGTTCCATCTGGCAGGCCATTTTGACCTGCGGGTAGAGCGCCGGGTTCC                                                             >  SRR4302537.1559342/1‑94 (MQ=60)

                                  TCTTCATCGTGCCGAAGGCTGCAGCCGTCAGCAGATGGCTGGTCAGTTCCATCTGGCAGGCCATTTTGACCTGCGGGTAGAGCGCCGGGTTCCC                                                            <  SRR4302537.6501646/94‑1 (MQ=60)

                                   CTTCATCGTGCCGAAGGCTGCAGCCGTCAGCAGATGGCTGGTCAGTTCCATCTGGCAGGCCATTTTGACCTGCGGGTAGAGCGCCGGGTTCCCG                                                           <  SRR4302537.5081481/94‑1 (MQ=60)

                                    TTCATCGTGCCGAAGGCTGCAGCCGTCAGCAGATGGCTGGTCAGTTCCATCTGGCAGGCCATTTTGACCTGCGGGTAGAGCGCCGGGTTCCCGG                                                          <  SRR4302537.1316149/94‑1 (MQ=60)

                                      CATCGTGCCGAAGGCTGCAGCCGTCAGCAGATGGCTGGTCAGTTCCATCTGGCAGGCCATTTTGTCCTGCGGGTAGAGCGCCGGGGTCCCGGCT                                                        >  SRR4302537.1175639/1‑94 (MQ=60)

                                      CATCGTGCCGAAGGCTGCAGCCGTCAGCAGATGGCTGGTCAGTTCCATCTGGCAGGCCATTTTGACCTGCGGGTAGAGCGCCGGGTTCCCGGCA                                                        >  SRR4302537.4498655/1‑94 (MQ=60)

                                       ATCGTGCCGAAGGCTGCAGCCGTCAGCAGATGGCTGGTCAGTTCCATCTGGCAGGCCATTTTGACCTGCGGGTAGAGCGCCGGGTTCCCGGCAT                                                       <  SRR4302537.1099972/94‑1 (MQ=60)

                                        TCGTGCCGAAGGCTGCAGCCGTCAGCAGATGGCTGGTCAGTTCCATCTGGCAGGCCATTTTGACCTGCGGGTAGAGCGCCGGGTTCCCGGCATG                                                      <  SRR4302537.6297443/94‑1 (MQ=60)

                                          GTGCCGAAGGGTGCAGCCGTCAGCAGATGGCTGGTCAGTTCCATCTGGCAGGCCATTTTGACCTGCGGGTAGAGCGCCGGGTTCCCGGCATGTG                                                    <  SRR4302537.6332822/94‑1 (MQ=60)

                                          GTGCCGAAGGCTGCAGCCGTCAGCAGATGGCTGGTCAGTTCCATCTGGCAGGCCATTTTGACCTGCGGGTAGAGCGCCGGGTTCCCGGCATGTG                                                    >  SRR4302537.207665/1‑94 (MQ=60)

                                           TGCCGAAGGCTGCAGCCGTCAGCAGATGGCTGGTCAGTTCCATCTGGCAGGCCATTTTGACCTGCGGGTAGAGCGCCGGGTTCCCGGCATGTGT                                                   <  SRR4302537.437037/94‑1 (MQ=60)

                                           TGCCGAAGGCTGCAGCCGTCAGCAGATGGCTGGTCAGTTCCATCTGGCAGGCCATTTTGACCTGCGGGTAGAGCGCCGGGTTCCCGGCATGTGT                                                   >  SRR4302537.3090856/1‑94 (MQ=60)

                                            GCCGAAGGCTGCAGCCGTCAGCAGATGGCTGGTCAGTTCCATCTGGCAGGCCATTTTGACCTGCGGGTAGAGCGCCGGGTTCCCGGCATGTGTC                                                  >  SRR4302537.5342028/1‑94 (MQ=60)

                                            GCCGAAGGCGGCAGCCGTCAGCAGATGGCTGGTCAGTTCCATCTGGCAGGCCATTTTGACCTGCGGGTAGAGCGCCGGGTTCCCGGCATGTGTC                                                  <  SRR4302537.1209614/94‑1 (MQ=60)

                                             CCGAAGGCTGCAGCCGTCAGCAGATGGCTGGTCAGTTCCATCTGGCAGGCCATTTTGACCTGCGGGTAGAGCGCCGGGTTCCCGGCATGTGTCT                                                 <  SRR4302537.721435/94‑1 (MQ=60)

                                             CCGAAGGCTGCAGCCGTCAGCAGATGGCTGGTCAGTTCCATCTGGCAGGCCATTTTGACCTGCGGGTAGAGCGACGGGTTCCCGGCATGTGTCT                                                 >  SRR4302537.5529749/1‑94 (MQ=60)

                                              CGAAGGCTGCAGCCGTCAGCAGATGGCTGGTCAGTTCCATCTGGCAGGCCATTTTGACCTGCGGGTAGAGCGCCGGGTTCCTGGCATGTGTCTG                                                >  SRR4302537.1047342/1‑94 (MQ=60)

                                              CGAAGGCTGCAGCCGTCAGCAGATGGCTGGTCAGTTCCATCTGGCAGGCCATTTTGACCTGCGGGTAGAGCGCCGGGTTCCCGGCATGTGTCTG                                                >  SRR4302537.2857771/1‑94 (MQ=60)

                                              CGAAGGCTGCAGCCGTCAGCAGATGGCTGGTCAGTTCCATCTGGCAGGCCATTTTGACCTGCGGGTAGAGCGCCGGGTTCCCGGCATGTGTCTG                                                <  SRR4302537.709755/94‑1 (MQ=60)

                                              CGAAGGCTGCAGCCGTCAGCAGATGGCTGGTCAGTTCCATCTGGCAGGCCATTTTGACCTGCGGGTAGAGCGCCGGGTTCCCGGCATGTGTCTG                                                >  SRR4302537.2682366/1‑94 (MQ=60)

                                              CGAAGGCTGCAGCCGTCAGCAGATGGCTGGTCAGTTCCATCTGGCAGGCCATGTTGACCTGCGGGTAGAGCGCCGGGTTCCCGGCATGTGTCTG                                                <  SRR4302537.3051218/94‑1 (MQ=60)

                                                AAGGCTGCAGCCGTCAGCAGATGGCTGGTCAGTTCCATCTGGCAGGCCATTTTGACCTGCGGGTAGAGCGCCGGGTTCCCGGCATGTGTCTGGC                                              >  SRR4302537.5640919/1‑94 (MQ=60)

                                                 AGGCTGCAGCCGTCAGCAGATGGCTGGTCAGTTCCATCTGGCAGGCCATTTTGACCTGCGGGTAGAGCGCCGGGGTCCCGGCATGTGTGTGGCG                                             >  SRR4302537.5776883/1‑94 (MQ=60)

                                                  GGCTGCAGCCGTCAGCAGATGGCTGGTCAGTTCCATCTGGCAGGCCATTTTGACCTGCGGGTAGAGCGCCGGGTTCCCGGCATGTGTCTGGCGG                                            >  SRR4302537.237313/1‑94 (MQ=60)

                                                   GCTGCAGCCGTCAGCAGATGGCTGGTCAGTTCCATCTGGCAGGCCATTTTGACCTGCGGGTAGAGCGCCGGGTTCCCGGCATGTGTCTGGCGGG                                           <  SRR4302537.1656931/94‑1 (MQ=60)

                                                   GCTGCAGCCGTCAGCAGATGGCTGGTCAGTTCCATCTGGCAGGCCATTTTGACCTGCGGGTAGAGCGCCGGGTTCCCGGCATGTGTCTGGCGGG                                           <  SRR4302537.2978467/94‑1 (MQ=60)

                                                   GCTGCAGCCGTCAGCAGATGGCTGGTCAGTTCCATCTGGCAGGCCATTTTGACCTGCGGGTAGAGCGCCGGGTTCCCGGCATGTGTCTGGCGGG                                           >  SRR4302537.3522203/1‑94 (MQ=60)

                                                    CTGCAGCCGTCAGCAGATGGCTGGTCAGTTCCATCTGGCAGGCCATTTTGACCTGCGGGTAGAGCGCCGGGTTCCCGGCATGTGTCTGGCGGGG                                          >  SRR4302537.5788824/1‑94 (MQ=60)

                                                    CTGCAGCCGTCAGCAGATGGCTGGTCAGTTCCATCTGGCAGGCCATTTTGACCTGCGGGTAGAGCGCCGGGTTCCCGGCATGTGTCTGGCGGGG                                          >  SRR4302537.193216/1‑94 (MQ=60)

                                                      GCAGCCGTCAGCAGATGGCTGGTCAGTTCCATCTGGCAGGCCATTTTGACCTGCGGGTAGAGCGCCGGGTTCCCGGCATGTGTCTGGCGGGGGA                                        <  SRR4302537.1116017/94‑1 (MQ=60)

                                                      GCAGCCGTCAGCAGATGGCTGGTCAGTTCCATCTGGCAGGCCATTTTGACCTGCGGGTAGAGCGCCGGGTTCCCGGCATGTGTCTGGCGGGGGA                                        >  SRR4302537.1683145/1‑94 (MQ=60)

                                                      GCAGCCGTCAGCAGATGGCTGGTCAGTTCCATCTGGCAGGCCATTTTGACCTGCGGGTAGAGCGCCGGGTTCCCGGCATGTGTCTGGCGGGGGA                                        <  SRR4302537.4935973/94‑1 (MQ=60)

                                                      GCAGCCGTCAGCAGATGGCTGGTCAGTTCCATCTGGCAGGCCATTTTGACCTGCGGGTAGAGCGCCGGGTTCCCGGCATGTGTCTGGCGGGGGA                                        >  SRR4302537.5621910/1‑94 (MQ=60)

                                                        AGCCGTCAGCAGATGGCTGGTCAGTTCCATCTGGCAGGCCATTTTGACCTGCGGGTAGAGCGCCGGGTTCCCGGCATGTGTCTGGCGGGGGAAG                                      >  SRR4302537.198381/1‑94 (MQ=60)

                                                        AGCCGTCAGCAGATGGCTGGTCAGTTCCATCTGGCAGGCCATTTTGACCTGCGGGTAGAGCGCCGGGTTCCCGGCATGTGTCTGGCGGGGGAAG                                      >  SRR4302537.2684816/1‑94 (MQ=60)

                                                         GCCGTCAGCAGATGGCTGGTCAGTTCCATCTGGCAGGCCATTTTGACCTGCGGGTAGAGCGCCGGGTTCCCGGCATGTGTCTGGCGGGGGAAGG                                     <  SRR4302537.215017/94‑1 (MQ=60)

                                                            GTCAGCAGATGGCTGGTCAGTTCCATCTGGCAGGCCATTTTGACCTGCGGGTAGAGCGCCGGGTTCCCGGCATGTGTCTGGCGGGGGAAGGCTG                                  >  SRR4302537.2247014/1‑94 (MQ=60)

                                                            GTCAGCAGATGGCTGGTCAGTTCCATCTGGCAGGCCATTTTGACCTGCGGGTAGAGCGCCGGGTTCCCGGCATGTGTCTGGCGGGGGAAGGCTG                                  >  SRR4302537.2192639/1‑94 (MQ=60)

                                                              CAGCAGATGGCTGGTCAGTTCCATCTGGCAGGCCATTTTGACCTGCGGGTAGAGCGCCGGGTTCCCGGCATGTGTCTGGCGGGGGAAGGCTGCA                                >  SRR4302537.2952270/1‑94 (MQ=60)

                                                              CAGCAGATGGCTGGTCAGTTCCATCTGGCAGGCCATTTTGACCTGCGGGTAGAGCGCCGGGTTCCCGGCATGTGTCTGGCGGGGGAAGGCTGCA                                <  SRR4302537.2793199/94‑1 (MQ=60)

                                                               AGCAGATGGCTGGTCAGTTCCATCTGGCAGGCCATTTTGACCTGCGGGTAGAGCGCCGGGTTCCCGGCATGTGTCTGGCGGGGGAAGGCTGCAT                               >  SRR4302537.3799683/1‑94 (MQ=60)

                                                               AGCAGATGGCTGGTCAGTTCCATCTGGCAGGCCATTTTGACCTGCGGGTAGAGCGCCGGGTTCCCGGCATGTGTCTGGCGGGGGAAGGCTGCAT                               >  SRR4302537.1887676/1‑94 (MQ=60)

                                                                 CAGATGGCTGGTCAGTTCCATCTGGCAGGCCATTTTGACCTGCTGGTAGAGCGCCGGGTTCCCGGCATGTGTCTGGCGGGGGAAGGCTGCATCG                             <  SRR4302537.3728401/94‑1 (MQ=60)

                                                                 CAGATGGCTGGTCAGTTCCATCTGGCAGGCCATTTTGACCTGCGGGTAGAGCGCCGGGTTCCCGGCATGTGTCTGGCGGGGGGAGGCTGCATCG                             >  SRR4302537.5547187/1‑94 (MQ=60)

                                                                 CAGATGGCGGGTCAGTTCCATCTGGCAGGCCATTTTGACCTGCTGGTAGAGCGCCGGGTTCCCGGCATGTGTCTGGCGGGGGAAGGCTGCATCG                             <  SRR4302537.4648131/94‑1 (MQ=60)

                                                                    ATGGCTGGTCAGTTCCATCTGGCAGGCCATTTTGACCTGCGGGTAGAGGGCCGGGTTCCCGGCATGTGGCTGGCGGGGGAAGGGTGCATCGTTC                          >  SRR4302537.4642571/1‑94 (MQ=60)

                                                                    ATGGCTGGTCAGTTCCATCTGGCAGGCCATTTTGACCTGCGGGTAGAGCGCCGGGTTCCCGGCATGTGTCTGGCGGGGGAAGGCTGCATCGTTC                          >  SRR4302537.2218199/1‑94 (MQ=60)

                                                                    ATGGCTGGTCAGTTCCATCTGGCAGGCCATTTTGACCTGCGGGTAGAGCGCCGGGTTCCCGGCATGTGTCTGGCGGGGGAAGGCTGCATCGTTC                          >  SRR4302537.780415/1‑94 (MQ=60)

                                                                     TGGCTGGTCAGTTCCATCTGGCAGGCCATTTTGACCTGCGGGTAGAGCGCCGGGTTCCCGGCATGTGTCTGGCGGGGGAAGGCTGCATCGTTCT                         <  SRR4302537.2944342/94‑1 (MQ=60)

                                                                     TGGCTGGTCAGTTCCATCTGGCAGGCCATTTTGACCTGCGGGTAGAGCGCCGGGTTCCCGGCATGTGTCTGGCGGGGGAAGGCTGCATCGTTCT                         <  SRR4302537.4910250/94‑1 (MQ=60)

                                                                     TGGCTGGTCAGTTCCATCTGGCAGGCCATTTTGACCTGCGGGTAGAGCGCCGGGTTCCCGGCATGTGTCTGGCGGGGGAAGGCTGCATCGTTCT                         >  SRR4302537.5711295/1‑94 (MQ=60)

                                                                       GCTGGTCAGTTCCATCTGGCAGGCCATTTTGACCTGCGGGTAGAGCGCCGGGTTCCCGGCATGTGTCTGGCGGGGGAAGGCTGCATCGTTCTCT                       <  SRR4302537.5622940/94‑1 (MQ=60)

                                                                        CTGGTCAGTTCCATCTGGCAGGCCATTTTGACCTGCGGGTAGAGCGCCGGGTTCCCGGCATGTGTCTGGCGGGGGAAGGCTGCATCGTTCTCTG                      <  SRR4302537.5804627/94‑1 (MQ=60)

                                                                         TTGTCGGTTCCATCTGGGAGGCCATTTTGACCTGCGGGTAGAGCGCCGGGTTCCCGGCATGTGTCTGGCGGGGGAAGGCTGCATCGTTCTCTGG                     <  SRR4302537.1718025/94‑1 (MQ=60)

                                                                          GGTCAGTTCCATCTGGCAGGCCATTTTGACCTGCGGGTAGAGCGCCGGGTTCCCGGCATGTGTCTGGCGGGGGAAGGCTGCATCGTTCTCTGGT                    <  SRR4302537.3000952/94‑1 (MQ=60)

                                                                          GGTCAGTTCCATCTGGCAGGCCATTTTGACCTGCGGGTAGAGCGCCGGGTTCCCGGCATGTGTCTGGCGGGGGAAGGCTGCATCGTTCTCTGGT                    >  SRR4302537.796194/1‑94 (MQ=60)

                                                                          GGTCAGTTCCATCTGGCAGGCCATTTTGACCTGCGGGTAGAGCGCCGGGTTCCCGGCATGTGTCTGGCGGGGGAAGGCTGCATCGTTCTCTGGT                    <  SRR4302537.6365091/94‑1 (MQ=60)

                                                                           GTCAGTTCCATCTGGCAGGCCATTTTGACCTGCGGGTAGAGCGCCGGGTTCCCGGCATGTGTCTGGCGGGGGAAGGCTGCATCGTTCTCTGGTG                   <  SRR4302537.7863/94‑1 (MQ=60)

                                                                            TCAGTTCCATCTGGCAGGCCATTTTGACCTGCGGGTAGAGCGCCGGGTTCCCGGCATGTGTCTGGCGGGGGAAGGCTGCATCGTTCTCTGGTGT                  >  SRR4302537.4893316/1‑94 (MQ=60)

                                                                            TCAGTTCCATCTGGCAGGCCATTTTGACCTGCGGGTAGAGCGCCGGGTTCCCGGCATGTGTCTGGCGGGGGAAGGCTGCATCGTTCTCTGGTGT                  <  SRR4302537.5018998/94‑1 (MQ=60)

                                                                              AGTTCCATCTGGCAGGCCATTTTGACCTGCGGGTAGAGCGCCGGGTTCCCGGCATGTGTCTGGCGGGGGAAGGCTGCATCGTTCTCTGGTGTAT                <  SRR4302537.176193/94‑1 (MQ=60)

                                                                              AGTTCCATCTGGCAGGCCATTTTGACCTGCGGGTAGAGCGCCGGGTTCCCGGCATGTGTCTGGCGGGGGAAGGCTGCATCGTTCTCTGGTGTAT                >  SRR4302537.4191194/1‑94 (MQ=60)

                                                                              AGTTCCATCTGGCAGGCCATTTTGACCTGCGGGTAGAGCGCCGGGTTCCCGGCATGTGTCTGGCGGGGGAAGGCTGCATCGTTCTCTGGTGTAT                <  SRR4302537.6055587/94‑1 (MQ=60)

                                                                              AGTTCCATCTGGCAGGCCATTTTGACCTGCGGGTAGAGCGCCGGGTTCCCGGCATGTGTCTGGCGGGGGAAGGCTGCATCGTTCTCTGGTGTAT                <  SRR4302537.6018489/94‑1 (MQ=60)

                                                                               GTTCCATCTGGCAGGCCATTTTGACCTGCGGGTAGAGCGCCGGGTTCCCGGCATGTGTCTGGCGGGGGACGGCTGCATCATTCTCTGGTGTATC               >  SRR4302537.1367554/1‑94 (MQ=60)

                                                                               GTTCCATCTGGCAGGCCATTTTGACCTGCGGGTAGAGCGCCGGGTTCCCGGCATGTGTCTGGCGGGGGAAGGCTGCATCGTTCTCTGGTGTATC               >  SRR4302537.1361435/1‑94 (MQ=60)

                                                                                TTCCATCTGTCAGGCCATTTTGACCTGCGGGTAGAGCGCCGGGTTCCCGGCATGTGTCTGGCGGGGGAAGGCTGCATCGTTCTCTGGTGTATCC              <  SRR4302537.3147981/94‑1 (MQ=60)

                                                                                TTCCATCTGGCAGGCCATTTTGACCTGCGGGTAGAGCGCCGGGTTCCCGGCATGTGTCTGGCGGGGGAAGGCTGCATCGTTCTCTGGTGTATCC              <  SRR4302537.5148465/94‑1 (MQ=60)

                                                                                TTCCATCTGGCAGGCCATTTTGACCTGCGGGTAGAGCGCCGGGTTCCCGGCATGTGTCTGGCGGGGGAAGGCTGCATCGTTCTCTGGTGTATCC              <  SRR4302537.3682809/94‑1 (MQ=60)

                                                                                TTCCATCTGGCAGGCCATTTTGACCTGCGGGTAGAGCGCCGGGTTCCCGGCATGTGTCTGGCGGGGGAAGGCTGCATCGTTCTCTGGTGTATCC              <  SRR4302537.218744/94‑1 (MQ=60)

                                                                                 TCCATCTGGCAGGCCATTTTGACCTGCGGGTAGAGCGCCGGGTTCCCGGCATGTGTCTGGCGGGGGAAGGCTGCATCGTTCTCTGGTGTATCCG             <  SRR4302537.1252776/94‑1 (MQ=60)

                                                                                 TCCATCTGGCAGGCCATTTTGACCTGCGGGTAGAGCGCCGGGTTCCCGGCATGTGTCTGGCGGGGGAAGGCTGCATCGTTCTCTGGTGTATCCG             >  SRR4302537.3280036/1‑94 (MQ=60)

                                                                                 TCCATCTGGCAGGCCATTTTGACCTGCGGGTAGAGCGCCGGGTTCCCGGCATGTGTCTGGCGGGGGAAGGCTGCATCGTTCTCTGGTGTATCCG             >  SRR4302537.2336600/1‑94 (MQ=60)

                                                                                  CCATCTGGCAGGCCATTTTGACCTGCGGGTAGAGCGCCGGGTTCCCGGCATGTGTCTGGCGGGGGAAGGCTGCATCGTTCTCTGGTGTATCCGG            >  SRR4302537.2991978/1‑94 (MQ=60)

                                                                                  CCATCTGGCAGGCCATTTTGACCTGCGGGTAGAGCGCCGGGTTCCCGGCATGTGTCTGGCGGGGGAAGGCTGCATCGTTCTCTGGTGTATCCGG            >  SRR4302537.5897820/1‑94 (MQ=60)

                                                                                  CCATCTGGCAGGCCATTTTGACCTGCGGGTAGAGCGCCGGGTTCCCGGCATGTGTCTGGCGGGGGAAGGCTGCATCGTTCTCTGGTGTATCCGG            <  SRR4302537.3804370/94‑1 (MQ=60)

                                                                                   CATCTGGCAGGCCATTTTGACCTGCGGGTAGAGCGCCGGGTTCCCGGCATGTGTCTGGCGGGGGAAGGCTGCATCGTTCTCTGGTGTATCCGGT           >  SRR4302537.110122/1‑94 (MQ=60)

                                                                                   CATCTGGCAGGCCATTTTGACCTGCGGGTAGAGCGCCGGGTTCCCGGCATGTGTCTGGCGGGGGAAGGCTGCATCGTTCTCTGGTGTATCCGGT           >  SRR4302537.2555017/1‑94 (MQ=60)

                                                                                   CATCTGGCAGGCCATTTTGACCTGCGGGTAGAGCGCCGGGTTCCCGGCATGTGTCTGGCGGGGGAAGGCTGCATCGTTCTCTGGTGTATCCGGT           >  SRR4302537.2402609/1‑94 (MQ=60)

                                                                                    ATCTGGCAGGCCATTTTGACCTGCGGGTAGAGCGCCGGGTTCCCGGCATGTGTCTGGCGGGGGAAGGCTGCATCGTTCTCTGGTGTATCCGGTG          <  SRR4302537.4925352/94‑1 (MQ=60)

                                                                                    ATCTGGCAGGCCATTTTGACCTGCGGGTAGAGCGCCGGGTTCCCGGCATGTGTCTGGCGGGGGAAGGCTGCATCGTTCTCTGGTGTATCCGGTG          <  SRR4302537.1421867/94‑1 (MQ=60)

                                                                                       TGGCAGGCCATTTTGACCTGCGGGTAGAGCGCCGGGTTCCCGGCATGTGTCTGGCGGGGGAAGGCTGCATCGTTCTCTGGTGTATCCGGTGTGC       <  SRR4302537.459095/94‑1 (MQ=60)

                                                                                        GGCAGGCCATTTTGACCTGCGGGTAGAGCGCCGGGTTCCCGGCATGTGTCTGGCGGGGGAAGGCTGCATCGTTCTCTGGTGTATCCGGTGTGCG      >  SRR4302537.2389694/1‑94 (MQ=60)

                                                                                        GGCAGGCCATTTTGACCTGCGGGTAGAGCGCCGGGTTCCCGGCATGTGTCTGGCGGGGGAAGGCTGCATCGTTCTCTGGTGTATCCGGTGTGCG      <  SRR4302537.2911887/94‑1 (MQ=60)

                                                                                         GCAGGCCATTTTGACCTGCGGGTAGAGCGCCGGGTTCCCGGCATGTGTCTGGCGGGGGAAGGCTGCGTCGTTCTCTGGTGTATCCGGTGTGCGC     >  SRR4302537.1691441/1‑94 (MQ=60)

                                                                                         GCAGGCCATTTTGACCTGCGGGTAGAGCGCCGGGTTCCCGGCATGTGTCTGGCGGGGGAAGGCTGCATCGTTCTCTGGTGTATCCGGTGTGCGC     <  SRR4302537.373181/94‑1 (MQ=60)

                                                                                          CAGGCCATTTTGACCTGCGGGTAGAGCGCCGGGTTCCCGGCATGTGTCTGGCGGGGGGAGGCTGCATCGTTCTCTGGTGTATCCGGGGTGCGCC    >  SRR4302537.2284791/1‑94 (MQ=60)

                                                                                          CAGGCCATTTTGACCTGCGGGTAGAGCGCCGGGTTCCCGGCATGTGTCTGGCGGGGGAAGGCTGCATCGTTCTCTGGTGTATCCGGTGTGCGCC    >  SRR4302537.4756478/1‑94 (MQ=60)

                                                                                          CAGGCCATTTTGACCTGCGGGTAGAGCGCCGGGTTCCCGGCATGTGTCTGGCGGGGGAAGGCTGCATCGTTCTCTGGTGTATCCGGTGTGCGCC    <  SRR4302537.3434463/94‑1 (MQ=60)

                                                                                           AGGCCATTTTGACCTGCGGGTAGAGCGCCGGGTTCCCGGCATGTGTCTGGCGGGGGAAGGCTGCATCGTTCTCTGGTGTATCCGGTGTGCGCCA   >  SRR4302537.4175940/1‑94 (MQ=60)

                                                                                           AGGCCATTTTGACCTGCGGGTAGAGCGCCGGGTTCCCGGCATGTGTCTGGCGGGGGAAGGCTGCATCGTTCTCTGGTGTATCCGGTGTGCGCCA   >  SRR4302537.2946859/1‑94 (MQ=60)

                                                                                            GGCCATTTTGACCTGCGGGTAGAGCGCCGGGTTCCCGGCATGTGTCTGGCGGGGGAAGGCTGCATCGTTCTCTGGTGTATCCGGTGTGCGCCAG  <  SRR4302537.2062327/94‑1 (MQ=60)

                                                                                            GGCCATTTTGACCTGCGGGTAGAGCGCCGGGTTCCCGGCATGTGTCTGGCGGGGGAAGGCTGCATCGTTCTCTGGTGTATCCGGTGTGCGCCAG  >  SRR4302537.909121/1‑94 (MQ=60)

TCTATAAGTTGCTCAGCAAGCTCATTTTCGCTGTTCTTCATCGTGCCGAAGGCTGCAGCCGTCAGCAGATGGCTGGTCAGTTCCATCTGGCAGACCATTTTGACCTGCGGGTAGAGCGCCGGGTTCCCGGCATGTGTCTGGCGGGGGAAGGCTGCATCGTTCTCTGGTGTATCCGGTGTGCGCCAG  >  NC_000913/4500407‑4500592

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 6 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`