Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 882 254.4 12957588 98.0% 12698436 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,593,336 +G intergenic (‑23/‑208) yjjM ← / → yjjN hypothetical protein/putative oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,593,3351.G99.6% 813.4 / ‑4.2 233intergenic (‑22/‑209)yjjM/yjjNhypothetical protein/putative oxidoreductase
Reads supporting (aligned to +/- strand):  ref base . (0/1);  new base G (116/116);  total (116/117)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 258 total aligned reads displayed.

TGCGTCAAGACCGCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGA‑T‑GAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAAATTTTGTGAACGACTCATAT  >  NC_000913/4593243‑4593422
                                                                                              |                                                                                       
tGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGA‑t                                                                                          <  1:963242/93‑1 (MQ=255)
tGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGA‑t                                                                                          >  1:1842892/1‑93 (MQ=255)
 gCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGA‑T‑g                                                                                        <  1:4485017/93‑1 (MQ=255)
 gCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGA‑T‑g                                                                                        >  1:4179552/1‑93 (MQ=255)
   gTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGA‑T‑gga                                                                                      <  2:1394174/93‑3 (MQ=255)
   gTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGA‑T‑gga                                                                                      <  1:5777752/93‑3 (MQ=255)
    tCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGA‑T‑ggaa                                                                                     >  2:4343298/1‑90 (MQ=255)
    tCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGA‑T‑ggaa                                                                                     >  2:2946660/1‑90 (MQ=255)
    tCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGA‑T‑ggaa                                                                                     >  1:4990346/1‑90 (MQ=255)
     cAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGA‑T‑ggaag                                                                                    <  1:4519484/93‑5 (MQ=255)
          cgccgcATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGA‑TGGAAGTgggg                                                                                <  2:5385053/90‑1 (MQ=255)
           gccgcATTCGCGTAAGTGGCTGAGAATATGTCGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGA‑TGGAAGTGGGGa                                                                               >  2:5324137/3‑93 (MQ=255)
           gccgcATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGA‑TGGAAGTGGGGa                                                                               >  2:1138690/3‑93 (MQ=255)
            ccgcATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGA‑TGGAAGTGGGGaa                                                                              <  2:5896687/92‑1 (MQ=255)
            ccgcATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGA‑TGGAAGTGGGGaa                                                                              >  1:3212692/2‑93 (MQ=255)
             cgcATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGA‑TGGAAGTGGGGAAg                                                                             <  2:4442659/93‑1 (MQ=255)
              gcATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGA‑TGGAAGTGGGGAAgg                                                                            <  2:1050142/93‑1 (MQ=255)
              gcATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGA‑TGGAAGTGGGGAAgg                                                                            <  1:5225673/93‑1 (MQ=255)
              gcATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGA‑TGGAAGTGGGGAAgg                                                                            <  2:4477947/93‑1 (MQ=255)
              acATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGA‑TGGAAGTGGGGAAgg                                                                            >  2:6205204/2‑93 (MQ=255)
               cATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGA‑TGGAAGTGGGGAAggg                                                                           >  1:2847236/1‑93 (MQ=255)
                aTTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGA‑TGGAAGTGGGGAAGGGa                                                                          <  2:6243542/93‑1 (MQ=255)
                  tCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGA‑TGGAAGTGGGGAAGGGata                                                                        <  2:2514189/93‑1 (MQ=255)
                   cgcgTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGA‑TGGAAGTGGGGAAGGGatat                                                                       <  2:2171972/93‑1 (MQ=255)
                     cgTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGA‑TGGAAGTGGGGAAGGGATATGa                                                                     <  2:3476640/93‑1 (MQ=255)
                       tAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGA‑TGGAAGTGGGGAAGGGATATGAcc                                                                   <  2:5511854/93‑1 (MQ=255)
                       tAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGA‑TGGAAGTGGGGAAGGGATATGAcc                                                                   >  2:4180595/1‑93 (MQ=255)
                       tAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGA‑TGGAAGTGGGGAAGGGATATGAcc                                                                   >  1:3972419/1‑93 (MQ=255)
                        aaGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGA‑TGGAAGTGGGGAAGGGATATGAccc                                                                  <  2:3686080/93‑1 (MQ=255)
                        aaGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGA‑TGGAAGTGGGGAAGGGATATGAccc                                                                  <  2:2223129/93‑1 (MQ=255)
                           tGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGA‑TGGAAGTGGGGAAGGGATATGACCCcgc                                                               <  1:856443/93‑1 (MQ=255)
                              cTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGA‑TGGAAGTGGGGAAGGGATATGACCCCGCGcc                                                            <  1:3735786/93‑1 (MQ=255)
                              cTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGA‑TGGAAGTGGGGAAGGGATATGACCCCGCGcc                                                            >  1:337956/1‑93 (MQ=255)
                               tGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGA‑TGGAAGTGGGGAAGGGATATGACCCCGCGCCa                                                           <  1:672330/93‑1 (MQ=255)
                                gagaATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGA‑TGGAAGTGGGGAAGGGATATGACCCCGCGCCat                                                          >  1:1234098/1‑93 (MQ=255)
                                gagaATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGA‑TGGAAGTGGGGAAGGGATATGACCCCGCGCCat                                                          >  2:5614851/1‑93 (MQ=255)
                                gagaATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGA‑TGGAAGTGGGGAAGGGATATGACCCCGCGCCat                                                          >  2:3639039/1‑93 (MQ=255)
                                 agaATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGA‑TGGAAGTGGGGAAGGGATATGACCCCGCGCCata                                                         >  1:6205354/1‑93 (MQ=255)
                                 agaATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGA‑TGGAAGTGGGGAAGGGATATGACCCCGCGCCata                                                         >  1:5869639/1‑93 (MQ=255)
                                 agaATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGA‑TGGAAGTGGGGAAGGGATATGACCCCGCGCCata                                                         >  2:4220713/1‑93 (MQ=255)
                                  gaATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGA‑TGGAAGTGGGGAAGGGATATGACCCCGCGCCatat                                                        >  1:1179108/1‑93 (MQ=255)
                                  aaaTATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGA‑TGGAAGTGGGGAAGGGATATGACCCCGCGCCatat                                                        <  2:5294056/92‑1 (MQ=255)
                                    atatGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGA‑TGGAAGTGGGGAAGGGATATGACCCCGCGCCATatca                                                      <  1:1006535/93‑1 (MQ=255)
                                    atatGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGA‑TGGAAGTGGGGAAGGGATATGACCCCGCGCCATatca                                                      <  1:6004330/93‑1 (MQ=255)
                                    atatGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGA‑TGGAAGTGGGGAAGGGATATGACCCCGCGCCATatca                                                      <  1:1978275/93‑1 (MQ=255)
                                    atatGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGA‑TGGAAGTGGGGAAGGGATATGACCCCGCGCCATatca                                                      <  2:5992326/93‑1 (MQ=255)
                                     tatGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGA‑TGGAAGTGGGGAAGGGATATGACCCCGCGCCATatcat                                                     <  2:2660186/93‑1 (MQ=255)
                                     tatGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGA‑TGGAAGTGGGGAAGGGATATGACCCCGCGCCATatcat                                                     >  1:4819667/1‑93 (MQ=255)
                                     tatGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGA‑TGGAAGTGGGGAAGGGATATGACCCCGCGCCATatcat                                                     <  2:5114433/93‑1 (MQ=255)
                                      atGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGA‑TGGAAGTGGGGAAGGGATATGACCCCGCGCCATatcatc                                                    <  1:4739120/93‑1 (MQ=255)
                                      atGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGA‑TGGAAGTGGGGAAGGGATATGACCCCGCGCCATatcatc                                                    <  2:1644125/93‑1 (MQ=255)
                                       tGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGA‑TGGAAGTGGGGAAGGGATATGACCCCGCGCCATAtcatca                                                   <  2:4636889/93‑1 (MQ=255)
                                         aCGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGA‑TGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAAt                                                 <  1:2705263/93‑1 (MQ=255)
                                          cGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGA‑TGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATg                                                <  1:2793539/93‑1 (MQ=255)
                                           gCACTGTGGTGCGTCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGA‑TGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGa                                               <  1:2042004/93‑1 (MQ=255)
                                           gCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGA‑TGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGa                                               <  1:4001055/93‑1 (MQ=255)
                                            cACTGTGGTGCGTCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGA‑TGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGAc                                              <  2:1720696/93‑1 (MQ=255)
                                             aCTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGA‑TGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGAcc                                             <  2:2998744/93‑1 (MQ=255)
                                              cTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGA‑TGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCt                                            >  1:6097402/1‑93 (MQ=255)
                                                gtggtgCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGA‑TGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTgg                                          <  2:4280189/93‑1 (MQ=255)
                                                gtggtgCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGA‑TGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTgg                                          >  1:40365/1‑93 (MQ=255)
                                                 tggtgCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGA‑TGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGt                                         >  2:4799339/1‑93 (MQ=255)
                                                 gggtgCGGCTGATGTTGTACATTCCCGCAAGCGCACTTTGCGAGG‑GAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGt                                         <  1:1486586/92‑1 (MQ=255)
                                                  ggtgCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGA‑TGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGtt                                        >  1:4874945/1‑93 (MQ=255)
                                                    tgCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGA‑TGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTaa                                      <  2:4370193/93‑1 (MQ=255)
                                                     gCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGA‑TGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAAt                                     <  2:1234098/93‑1 (MQ=255)
                                                       ggCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGA‑TGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATca                                   >  2:2678818/1‑93 (MQ=255)
                                                         cTGATGTTGTACATTTCCGCCAGCGCACTTTGCGA‑TGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATcaca                                 >  1:3415585/1‑93 (MQ=255)
                                                          tGATGTTGTACATTTCCGCCAGCGCACTTTGCGA‑TGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACAt                                >  2:6403467/1‑93 (MQ=255)
                                                          tGATGTTGTACATTTCCGCCAGCGCACTTTGCGA‑TGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACAt                                >  1:231678/1‑93 (MQ=255)
                                                          tGATGTTGTACATTTCCGCCAGCGCACTTTGCGA‑TGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACAt                                <  1:5648144/93‑1 (MQ=255)
                                                            aTGTTGTACATTTCCGCCAGCGCACTTTGCGA‑TGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATtg                              >  2:677740/1‑93 (MQ=255)
                                                             tgttgtACATTTCCGCCAGCGCACTTTGCGA‑TGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATtgt                             <  1:1375782/93‑1 (MQ=255)
                                                              gttgtACATTTCCGCCAGCGCACTTTGCGA‑TGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATtgtg                            >  2:1415832/1‑93 (MQ=255)
                                                               ttgtACATTTCCGCCAGCGCACTTTGCGA‑TGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTgg                           <  2:5551975/93‑1 (MQ=255)
                                                               ttgtACATTTCCGCCAGCGCACTTTGCGA‑TGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTgg                           >  2:2028504/1‑93 (MQ=255)
                                                               ttgtACATTTCCGCCAGCGCACTTTGCGA‑TGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTgg                           >  1:6435606/1‑93 (MQ=255)
                                                               ttgtACATTTCCGCCAGCGCACTTTGCGA‑TGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTgg                           <  1:5526290/93‑1 (MQ=255)
                                                               ttgtACATTTCCGCCAGCGCACTTTGCGA‑TGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTgg                           <  1:4132326/93‑1 (MQ=255)
                                                                tgtACATTTCCGCCAGCGCACTTTGCGA‑TGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGc                          >  1:4688801/1‑93 (MQ=255)
                                                                 gtACATTTCCGCCAGCGCACTTTGCGA‑TGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCg                         >  2:3733536/1‑93 (MQ=255)
                                                                 gtACATTTCCGCCAGCGCACTTTGCGA‑TGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCg                         >  1:2721595/1‑93 (MQ=255)
                                                                    cATTTCCGCCAGCGCACTTTGCGA‑TGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTaa                      >  1:3890105/1‑93 (MQ=255)
                                                                    cATTTCCGCCAGCGCACTTTGCGA‑TGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTaa                      <  1:2056685/93‑1 (MQ=255)
                                                                      tttCCGCCAGCGCACTTTGCGA‑TGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAAAt                    >  2:1782625/1‑93 (MQ=255)
                                                                      tttCCGCCAGCGCACTTTGCGA‑TGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAAAt                    <  1:3257924/93‑1 (MQ=255)
                                                                      tttCCGCCAGCGCACTTTGCGA‑TGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAAAt                    >  1:1634463/1‑93 (MQ=255)
                                                                        tCCGCCAGCGCACTTTGCGA‑TGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAAAttt                  <  2:5869187/93‑1 (MQ=255)
                                                                        tCCGCCAGCGCACTTTGCGA‑TGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAAAttt                  <  2:5206746/93‑1 (MQ=255)
                                                                          cGCCAGCGCACTTTGCGA‑TGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAAATTTtg                >  1:1034715/1‑93 (MQ=255)
                                                                          cGCCAGCGCACTTTGCGA‑TGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAAATTTtg                <  1:2156016/93‑1 (MQ=255)
                                                                           gCCAGCGCACTTTGCGA‑TGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAAATTTtgt               >  2:4591345/1‑93 (MQ=255)
                                                                             cAGCGCACTTTGCGA‑TGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAAATTTTGTGa             >  2:5735703/1‑93 (MQ=255)
                                                                               tcgcACTTTGCGA‑TGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAAATTTTGTGAAc           <  1:768194/92‑1 (MQ=255)
                                                                               gcgcACTTTGCGA‑TGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAACCACATTGTGGCGTAAATTTTGTGAAc           <  2:3816340/93‑1 (MQ=255)
                                                                                 gcACTTTGCGA‑TGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAAATTTTGTGAACGa         >  1:1801829/1‑93 (MQ=255)
                                                                                 gcACTGTGCGA‑TGGAAGTGAGGAAGGTATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAAATTTTGTGAACGa         <  2:108132/93‑1 (MQ=255)
                                                                                   aCTTTGCGA‑TGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAAATTTTGTGAACGACt       >  1:2965949/1‑93 (MQ=255)
                                                                                    cTTTGCGA‑TGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAAATTTTGTGAACGACTc      >  2:191872/1‑93 (MQ=255)
                                                                                      ttGCGA‑TGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAAATTTTGTGAACGACTCat    >  2:834057/1‑93 (MQ=255)
                                                                                              |                                                                                       
TGCGTCAAGACCGCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGA‑T‑GAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAAATTTTGTGAACGACTCATAT  >  NC_000913/4593243‑4593422

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TGCGTCAAGACCGCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGA‑T‑GAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAAATTTTGTGAACGACTCATATTTTCCTC  >  NC_000913/4593243‑4593429
                                                                                               |                                                                                             
TGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGA‑T‑G                                                                                               <  SRR4302537.969890/94‑1 (MQ=60)
TGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGA‑T‑G                                                                                               >  SRR4302537.4208726/1‑94 (MQ=60)
 GCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGA‑T‑GG                                                                                              <  SRR4302537.4516043/94‑1 (MQ=60)
 GCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGA‑T‑GG                                                                                              <  SRR4302537.775805/94‑1 (MQ=60)
  CGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGA‑T‑GGA                                                                                             >  SRR4302537.5858814/1‑94 (MQ=60)
  CGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGA‑T‑GGA                                                                                             <  SRR4302537.4892105/94‑1 (MQ=60)
  CGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGA‑T‑GGA                                                                                             <  SRR4302537.5011532/94‑1 (MQ=60)
   GTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGA‑T‑Ggaa                                                                                            <  SRR4302537.5817357/94‑4 (MQ=60)
   GTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGA‑T‑Ggaa                                                                                            >  SRR4302537.5024739/1‑91 (MQ=60)
   GTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGA‑T‑Ggaa                                                                                            <  SRR4302537.1315085/94‑4 (MQ=60)
    TCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGA‑TGGAAG                                                                                            <  SRR4302537.3355006/94‑1 (MQ=60)
     CAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGA‑TGGAAGT                                                                                           <  SRR4302537.4550743/94‑1 (MQ=60)
      AAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGA‑TGGAAGTG                                                                                          <  SRR4302537.5798955/94‑1 (MQ=60)
      AAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGA‑TGGAAGTG                                                                                          >  SRR4302537.421280/1‑94 (MQ=60)
      AAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGA‑TGGAAGTG                                                                                          <  SRR4302537.635526/94‑1 (MQ=60)
      AAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGA‑TGGAAGTG                                                                                          >  SRR4302537.6501006/1‑94 (MQ=60)
       AGACTCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGA‑TGGAAGTGG                                                                                         <  SRR4302537.2780343/94‑1 (MQ=60)
       AGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGA‑TGGAAGTGG                                                                                         >  SRR4302537.1671167/1‑94 (MQ=60)
       AGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGA‑TGGAAGTGG                                                                                         >  SRR4302537.4950087/1‑94 (MQ=60)
        gacgcCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGA‑TGGAAGTGGG                                                                                        <  SRR4302537.707682/89‑1 (MQ=60)
        gacgcCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGA‑TGGAAGTGGG                                                                                        <  SRR4302537.4929947/89‑1 (MQ=60)
         acgcCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGA‑TGGAAGTGGGG                                                                                       <  SRR4302537.3419010/90‑1 (MQ=60)
           gcCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGA‑TGGAAGTGGGGAA                                                                                     >  SRR4302537.3235293/3‑94 (MQ=60)
           gcCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGA‑TGGAAGTGGGGAA                                                                                     <  SRR4302537.272805/92‑1 (MQ=60)
            CCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGA‑TGGAAGTGGGGAAG                                                                                    <  SRR4302537.2200233/94‑1 (MQ=60)
              GCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGA‑TGGAAGTGGGGAAGGG                                                                                  >  SRR4302537.2867169/1‑94 (MQ=60)
              GCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGA‑TGGAAGTGGGGAAGGG                                                                                  <  SRR4302537.5261608/94‑1 (MQ=60)
              GCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGA‑TGGAAGTGGGGAAGGG                                                                                  >  SRR4302537.920870/1‑94 (MQ=60)
                ATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGA‑TGGAAGTGGGGAAGGGAT                                                                                <  SRR4302537.6063748/94‑1 (MQ=60)
                ATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGA‑TGGAAGTGGGGAAGGGAT                                                                                <  SRR4302537.4801350/94‑1 (MQ=60)
                    GCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGA‑TGGAAGTGGGGAAGGGATATGA                                                                            >  SRR4302537.465503/1‑94 (MQ=60)
                      GTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGA‑TGGAAGTGGGGAAGGGATATGACC                                                                          >  SRR4302537.4000225/1‑94 (MQ=60)
                      GTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGA‑TGGAAGTGGGGAAGGGATATGACC                                                                          >  SRR4302537.4000226/1‑94 (MQ=60)
                      GTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGA‑TGGAAGTGGGGAAGGGATATGACC                                                                          >  SRR4302537.1521452/1‑94 (MQ=60)
                        AAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGA‑TGGAAGTGGGGAAGGGATATGACCCC                                                                        <  SRR4302537.1198353/94‑1 (MQ=60)
                          GTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGA‑TGGAAGTGGGGAAGGGATATGACCCCGC                                                                      >  SRR4302537.3848006/1‑94 (MQ=60)
                           TGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGA‑TGGAAGTGGGGAAGGGATATGACCCCGCG                                                                     >  SRR4302537.1282929/1‑94 (MQ=60)
                           TGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGA‑TGGAAGTGGGGAAGGGATATGACCCCGCG                                                                     <  SRR4302537.862321/94‑1 (MQ=60)
                           TGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGA‑TGGAAGTGGGGAAGGGATATGACCCCGCG                                                                     >  SRR4302537.5657793/1‑94 (MQ=60)
                             GCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGA‑TGGAAGTGGGGAAGGGATATGACCCCGCGCC                                                                   >  SRR4302537.340298/1‑94 (MQ=60)
                              CTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGA‑TGGAAGTGGGGAAGGGATATGACCCCGCGCCA                                                                  <  SRR4302537.3762024/94‑1 (MQ=60)
                               TGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGA‑TGGCAGTGGGGAAGGGATATGACCCCGCGCCAT                                                                 >  SRR4302537.467158/1‑94 (MQ=60)
                               TGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGA‑TGGAAGTGGGGAAGGGATATGACCCCGCGCCAT                                                                 <  SRR4302537.676932/94‑1 (MQ=60)
                               TGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGA‑TGGAAGTGGGGAAGGGATATGACCCCGCGCCAT                                                                 >  SRR4302537.1242679/1‑94 (MQ=60)
                                GAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGA‑TGGAAGTGGGGAAGGGATATGACCCCGCGCCATA                                                                >  SRR4302537.6248117/1‑94 (MQ=60)
                                GAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGA‑TGGAAGTGGGGAAGGGATATGACCCCGCGCCATA                                                                >  SRR4302537.5909903/1‑94 (MQ=60)
                                 AGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGA‑TGGAAGTGGGGAAGGGATATGACCCCGCGCCATAT                                                               >  SRR4302537.1187317/1‑94 (MQ=60)
                                 AGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGA‑TGGAAGTGGGGAAGGGATATGACCCCGCGCCATAT                                                               <  SRR4302537.2769974/94‑1 (MQ=60)
                                 AGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGA‑TGGAAGTGGGGAAGGGATATGACCCCGCGCCATAT                                                               <  SRR4302537.1962063/94‑1 (MQ=60)
                                  GAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGA‑TGGAAGTGGGGAAGGGATATGACCCCGCGCCATATC                                                              >  SRR4302537.5214865/1‑94 (MQ=60)
                                   AATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGA‑TGGAAGTGGGGAAGGGATATGACCCCGCGCCATCTCA                                                             >  SRR4302537.2056299/1‑94 (MQ=60)
                                    ATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGA‑TGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCAT                                                            <  SRR4302537.1013482/94‑1 (MQ=60)
                                    ATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGA‑TGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCAT                                                            <  SRR4302537.1992022/94‑1 (MQ=60)
                                    ATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGA‑TGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCAT                                                            >  SRR4302537.4852943/1‑94 (MQ=60)
                                    ATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGA‑TGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCAT                                                            <  SRR4302537.6045579/94‑1 (MQ=60)
                                     TATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGA‑TGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATC                                                           <  SRR4302537.4725582/94‑1 (MQ=60)
                                      ATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGA‑TGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCA                                                          <  SRR4302537.4771873/94‑1 (MQ=60)
                                      ATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGA‑TGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCA                                                          <  SRR4302537.6265657/94‑1 (MQ=60)
                                        GACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGA‑TGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAAT                                                        <  SRR4302537.1701932/94‑1 (MQ=60)
                                        GACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGA‑TGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAAT                                                        >  SRR4302537.2874297/1‑94 (MQ=60)
                                         ACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGA‑TGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATG                                                       <  SRR4302537.2724145/94‑1 (MQ=60)
                                         ACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGA‑TGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATG                                                       <  SRR4302537.4134760/94‑1 (MQ=60)
                                         ACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTGTGCGA‑TGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATG                                                       <  SRR4302537.3019207/94‑1 (MQ=60)
                                          CGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGA‑TGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGA                                                      >  SRR4302537.1716453/1‑94 (MQ=60)
                                          CGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGA‑TGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGA                                                      <  SRR4302537.2813065/94‑1 (MQ=60)
                                          CGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGA‑TGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGA                                                      >  SRR4302537.5776876/1‑94 (MQ=60)
                                           GCACTGTGGTGCGTCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGA‑TGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGAC                                                     <  SRR4302537.2056279/94‑1 (MQ=60)
                                           GCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGA‑TGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGAC                                                     <  SRR4302537.4029065/94‑1 (MQ=60)
                                            CACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGA‑TGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACC                                                    >  SRR4302537.2951354/1‑94 (MQ=60)
                                            CACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGA‑TGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACC                                                    >  SRR4302537.3444426/1‑94 (MQ=60)
                                             ACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGA‑TGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCT                                                   <  SRR4302537.49042/94‑1 (MQ=60)
                                             ACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGA‑TGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCT                                                   <  SRR4302537.702793/94‑1 (MQ=60)
                                             ACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGA‑TGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCT                                                   >  SRR4302537.3571289/1‑94 (MQ=60)
                                             ACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGA‑TGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCT                                                   >  SRR4302537.6139302/1‑94 (MQ=60)
                                               TGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGA‑TGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGG                                                 >  SRR4302537.40670/1‑94 (MQ=60)
                                               TGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGA‑TGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGG                                                 <  SRR4302537.5440211/94‑1 (MQ=60)
                                                GTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGA‑TGGAAGTGGGGAAGGGATATGGCCCCGCGCCATATCATCAATGACCTGGT                                                >  SRR4302537.1888866/1‑94 (MQ=60)
                                                GTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGA‑TGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGT                                                <  SRR4302537.2295632/94‑1 (MQ=60)
                                                 TGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGA‑TGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTT                                               <  SRR4302537.5982064/94‑1 (MQ=60)
                                                 TGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGA‑TGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTT                                               >  SRR4302537.4908592/1‑94 (MQ=60)
                                                 GGGTGCGGCTGATGTTGTACATTCCCGCAAGCGCACTTTGCGAGG‑GAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTT                                               <  SRR4302537.1496932/94‑1 (MQ=60)
                                                  GGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGA‑TGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTA                                              <  SRR4302537.4532289/94‑1 (MQ=60)
                                                    TGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGA‑TGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAAT                                            <  SRR4302537.2763048/94‑1 (MQ=60)
                                                    TGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGA‑TGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAAT                                            >  SRR4302537.3938258/1‑94 (MQ=60)
                                                     GCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGA‑TGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATC                                           <  SRR4302537.985860/94‑1 (MQ=60)
                                                     GCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGA‑TGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATC                                           <  SRR4302537.2141583/94‑1 (MQ=60)
                                                     GCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGA‑TGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATC                                           <  SRR4302537.118287/94‑1 (MQ=60)
                                                     GCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGA‑TGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATC                                           <  SRR4302537.3984064/94‑1 (MQ=60)
                                                        GCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGA‑TGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACA                                        >  SRR4302537.4008196/1‑94 (MQ=60)
                                                        GCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGA‑TGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACA                                        >  SRR4302537.3439770/1‑94 (MQ=60)
                                                         CTGATGTTGTACATTTCCGCCAGCGCACTTTGCGA‑TGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACAT                                       >  SRR4302537.1827056/1‑94 (MQ=60)
                                                         CTGATGTTGTACATTTCCGCCAGCGCACTTTGCGA‑TGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACAT                                       <  SRR4302537.5301058/94‑1 (MQ=60)
                                                         CTGATGTTGTACATTTCCGCCAGCGCACTTTGCGA‑TGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACAT                                       >  SRR4302537.233311/1‑94 (MQ=60)
                                                         CTGATGTTGTACATTTCCGCCAGCGCACTTTGCGA‑TGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACAT                                       >  SRR4302537.2606525/1‑94 (MQ=60)
                                                          TGATGTTGTACATTTCCGCCAGCGCACTTTGCGA‑TGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATT                                      <  SRR4302537.5686801/94‑1 (MQ=60)
                                                           GATGTTGTACATTTCCGCCAGCGCACTTTGCGA‑TGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTG                                     <  SRR4302537.6512991/94‑1 (MQ=60)
                                                            ATGTTGTACATTTCCGCCAGCGCACTTTGCGA‑TGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGT                                    >  SRR4302537.2821897/1‑94 (MQ=60)
                                                             TGTTGTACATTTCCGCCAGCGCACTTTGCGA‑TGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTG                                   <  SRR4302537.1385387/94‑1 (MQ=60)
                                                             TGTTGTACATTTCCGCCAGCGCACTTTGCGA‑TGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTG                                   >  SRR4302537.5161992/1‑94 (MQ=60)
                                                              GTTGTACATTTCCGCCAGCGCACTTTGCGA‑TGGAAGTGGGGAAGGTATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGG                                  <  SRR4302537.967056/94‑1 (MQ=60)
                                                              GTTGTACATTTCCGCCAGCGCACTTTGCGA‑TGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGG                                  >  SRR4302537.6479305/1‑94 (MQ=60)
                                                               TTGTACATTTCCGCTAGCGCACTTTGCGA‑TGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGC                                 <  SRR4302537.197250/94‑1 (MQ=60)
                                                               TTGTACATTTCCGCCAGCGCACTTTGCGA‑TGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGC                                 >  SRR4302537.4721236/1‑94 (MQ=60)
                                                               TTGTACATTTCCGCCAGCGCACTTTGCGA‑TGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGC                                 <  SRR4302537.4161166/94‑1 (MQ=60)
                                                               TTGTACATTTCCGCCAGCGCACTTTGCGA‑TGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGC                                 <  SRR4302537.5564010/94‑1 (MQ=60)
                                                                TGTACATTTCCGCCAGCGCACTTTGCGA‑TGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCG                                >  SRR4302537.2740599/1‑94 (MQ=60)
                                                                TGTACATTTCCGCCAGCGCACTTTGCGA‑TGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCG                                <  SRR4302537.5721822/94‑1 (MQ=60)
                                                                TGTACATTTCCGCCAGCGCACTTTGCGA‑TGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCG                                >  SRR4302537.4102576/1‑94 (MQ=60)
                                                                TGTACATTTCCGCCAGCGCACTTTGCGA‑TGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCG                                <  SRR4302537.3276752/94‑1 (MQ=60)
                                                                 GTACATTTCCGCCAGCGCACTTTGCGA‑TGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATTACCTGGTTAATCACATTGTGGCGT                               >  SRR4302537.4500930/1‑94 (MQ=60)
                                                                 GTACATTTCCGCCAGCGCACTTTGCGA‑TGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGT                               >  SRR4302537.118228/1‑94 (MQ=60)
                                                                  TACATGTCCGCCAGCTCGCGTTGCGA‑TGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTA                              <  SRR4302537.3057904/94‑1 (MQ=60)
                                                                   ACATTTCCGCCAGCGCACTTTGCGA‑TGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAA                             >  SRR4302537.3917312/1‑94 (MQ=60)
                                                                   ACATTTCCGCCAGCGCACTTTGCGA‑TGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAA                             >  SRR4302537.1922196/1‑94 (MQ=60)
                                                                    CATTTCCGCCAGCGCACTTTGCGA‑TGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAAA                            <  SRR4302537.3393294/94‑1 (MQ=60)
                                                                    CATTTCCGCCAGCGCACTTTGCGA‑TGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAAA                            >  SRR4302537.1584740/1‑94 (MQ=60)
                                                                    CATTTCCGCCAGCGCACTTTGCGA‑TGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAAA                            >  SRR4302537.6043648/1‑94 (MQ=60)
                                                                    CATTTCCGCCAGCGCACTTTGCGA‑TGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAAA                            <  SRR4302537.2071121/94‑1 (MQ=60)
                                                                     ATTTCCGCCAGCGCACTTTGCGA‑TGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAAAT                           >  SRR4302537.1645912/1‑94 (MQ=60)
                                                                      TTTCCGCCAGCGCACTTTGCGA‑TGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAAATT                          <  SRR4302537.3280893/94‑1 (MQ=60)
                                                                        TCCGCCAGCGCACTTTGCGA‑TGGCAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAAATTTT                        >  SRR4302537.4766732/1‑94 (MQ=60)
                                                                        TCCGCCAGCGCACTTTGCGA‑TGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAAATTTT                        >  SRR4302537.6153072/1‑94 (MQ=60)
                                                                        TCCGCCAGCGCACTTTGCGA‑TGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAAATTTT                        <  SRR4302537.2049071/94‑1 (MQ=60)
                                                                         CCGCCAGCGCACTTTGCGA‑TGGAAGTGGGGAAGGGATATGATCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAAATTCTG                       >  SRR4302537.1039557/1‑94 (MQ=60)
                                                                         CCGCCAGCGCACTTTGCGA‑TGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAAATTTTG                       >  SRR4302537.1041844/1‑94 (MQ=60)
                                                                          CGCCAGCGCACTTTGCGA‑TGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAAATTTTGT                      <  SRR4302537.2171212/94‑1 (MQ=60)
                                                                          CGCCAGCGCACTTTGCGA‑TGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAAATTTTGT                      >  SRR4302537.766505/1‑94 (MQ=60)
                                                                           GCCAGCGCACTTTGCGA‑TGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAAATTTTGTG                     >  SRR4302537.6298588/1‑94 (MQ=60)
                                                                            CCAGCGCACTTTGCGA‑TGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAAATTTTGTGA                    <  SRR4302537.6184566/94‑1 (MQ=60)
                                                                               TCGCACTTTGCGA‑TGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAAATTTTGTGAACG                 <  SRR4302537.773459/94‑1 (MQ=60)
                                                                                CGCACTTTGCGA‑TGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAAATTTTGTGAACGA                >  SRR4302537.1814398/1‑94 (MQ=60)
                                                                                CGCACTTTGCGA‑TGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAAATTTTGTGAACGA                >  SRR4302537.4033478/1‑94 (MQ=60)
                                                                                  CACTTTGCGA‑TGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAAATTTTGTGAACGACT              >  SRR4302537.2986755/1‑94 (MQ=60)
                                                                                   ACTTTGCGA‑TGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAAATTTTGTGAACGACTC             >  SRR4302537.4169802/1‑94 (MQ=60)
                                                                                    CTTTGCGA‑TGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAAATTTTGTGAACGACTCA            >  SRR4302537.2061491/1‑94 (MQ=60)
                                                                                       TGCGA‑TGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAAATTTTGTGAACGACTCATAT         <  SRR4302537.4406546/94‑1 (MQ=60)
                                                                                         CGA‑TGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAAATTTTGTGAACGACTCATATTT       >  SRR4302537.4114070/1‑94 (MQ=60)
                                                                                         CGA‑TGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAAATTTTGTGAACGACTCATATTT       <  SRR4302537.2757827/94‑1 (MQ=60)
                                                                                          GA‑TGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCGCATTGTGGCGTAAATTTTGTGAACGACTCATATTTT      <  SRR4302537.5050690/94‑1 (MQ=60)
                                                                                          GA‑TGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAAATTTTGTGAACGACTCATATTTT      >  SRR4302537.938608/1‑94 (MQ=60)
                                                                                             tgGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAAATTTTGTGAACGACTCATATTTTCC    <  SRR4302537.3399668/92‑1 (MQ=60)
                                                                                             tgGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAAATTTTGTGAACGACTCATATTTTCC    <  SRR4302537.323720/92‑1 (MQ=60)
                                                                                               GAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAAATTTTGTGAACGACTCATATTTTCCTC  <  SRR4302537.3572681/94‑1 (MQ=60)
                                                                                               GAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAAATTTTGTGAACGACTCATATTTTCCTC  <  SRR4302537.6433403/94‑1 (MQ=60)
                                                                                               |                                                                                             
TGCGTCAAGACCGCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGA‑T‑GAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAAATTTTGTGAACGACTCATATTTTCCTC  >  NC_000913/4593243‑4593429

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑