Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 882 254.4 12957588 98.0% 12698436 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,635,312 (A)9→8 coding (1048/1425 nt) creC → CreC

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,635,3040A.96.9% 1011.2 / 22.4 224coding (1040/1425 nt)creCCreC
Reads supporting (aligned to +/- strand):  ref base A (3/4);  new base . (94/123);  total (97/127)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.03e-01

Only 100 of 262 total aligned reads displayed.

ACTGGAGAATCGTCAGGAAGTCGTTCTGACTGCTGTTGATGTGGCGGCATTATTCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAACGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGAAATTTACTG  >  NC_000913/4635212‑4635392
                                                                                            |                                                                                        
 cTGGAGAATCGTCAGGAAGTCGTTCTGACTGCTGTTGATGTGGCGGCATTATTCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGaa                                                                                         <  2:4536187/93‑1 (MQ=255)
  tGGAGAATCGTCAGGAAGTCGTTCTGACTGCTGTTGATGTGGCGGCATTATTCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTTGCAGaaa                                                                                        >  1:2995558/1‑93 (MQ=255)
   ggAGAATCGTCAGGAAGTTGTTCTGACTGCTGTTGATGTGGCGGCATTATTCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGaaaa                                                                                       <  2:3168264/93‑1 (MQ=255)
   ggAGAATCGTCAGGAAGTCGTTCTGACTGCTGTTGATGTGGCGGCATTATTCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGaaaa                                                                                       <  1:36665/93‑1 (MQ=255)
     agaATCGTCAGGAAGTCGTTCTGACTGCTGTTGATGTGGCGGCATTATTCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGaaaaaa                                                                                     <  2:5141890/93‑1 (MQ=255)
       aaTCGTCAGGAAGTCGTTCTGACTGCTGTTGATGTGGCGGCATTATTCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGaaaaaaaa                                                                                   >  1:2043218/1‑93 (MQ=255)
         tCGTCAGGAAGTCGTTCTGACTGCTGTTGATGTGGCGGCATTATTCCGCCGCGTCAGCGAAGCGGGCACCGTGCAGTTGGCAGaaaaaaaatc                                                                                 >  1:2080535/1‑91 (MQ=255)
         tCGTCAGGAAGTCGTTCTGACTGCTGTTGATGTGGCGGCATTATTCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGaaaaaaaatc                                                                                 >  2:3929422/1‑91 (MQ=255)
         tCGTCAGGAAGTCGTTCTGACTGCTGTTGATGTGGCGGCATTATTCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGaaaaaaaatc                                                                                 <  1:2167197/93‑3 (MQ=255)
         tCGTCAGGAAGTCGTTCTGACTGCTGTTGATGTGGCGGCATTATTCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGaaaaaaaatc                                                                                 <  1:5876892/93‑3 (MQ=255)
          cGTCAGGAAGTCGTTCTGACTGCTGTTGATGTGGCGGCATTATTCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGaaaaaaaatca                                                                                <  2:588866/93‑4 (MQ=255)
          cGTCAGGAAGTCGTTCTGACTGCTGTTGATGTGGCGGCATTATTCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGaaaaaaaatca                                                                                <  2:1458455/93‑4 (MQ=255)
           gTCAGGAAGTCGTTCTGACTGCTGTTGATGTGGCGGCATTATTCCGGCGCGTCCGCGAAGCGCGCACCGTGGAGTTGGCAGaaaaaaaatcac                                                                               >  1:1954392/1‑89 (MQ=255)
           gTCAGGAAGTCGTTCTGACTGCTGTTGATGTGGCGGCATTATTCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGaaaaaaaatcac                                                                               <  1:1287786/93‑5 (MQ=255)
           gTCAGGAAGTCGTTCTGACTGCTGTTGATGTGGCGGCATTATTCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGaaaaaaaatcac                                                                               <  2:4326221/93‑5 (MQ=255)
             cAGGAAGTCGTTCTGACTGCTGTTGATGTGGCGGCATTATTCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAG‑AAAAAAAATCActc                                                                            <  2:2933445/93‑1 (MQ=255)
             cAGGAAGTCGTTCTGACTGCTGTTGATGTGGCGGCATTATTCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAG‑AAAAAAAATCActc                                                                            <  1:2800460/93‑1 (MQ=255)
              aGGAAGTCGTTCTGACTGCTGTTGATGTGGCGGCATTATTCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAG‑AAAAAAAATCActct                                                                           >  1:1666528/1‑93 (MQ=255)
              aGGAAGTCGTTCTGACTGCTGTTGATGTGGCGGCATTATTCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAG‑AAAAAAAATCActct                                                                           <  1:5456642/93‑1 (MQ=255)
              aGGAAGTCGTTCTGACTGCTGTTGATGTGGCGGCATTATTCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAG‑AAAAAAAATCActct                                                                           >  1:3336620/1‑93 (MQ=255)
              aGGAAGTCGTTCTGACTGCTGTTGATGTGGCGGCATTATTCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAG‑AAAAAAAATCActct                                                                           <  1:6402374/93‑1 (MQ=255)
              aGGAAGTCGTTCTGACTGCTGTTGATGTGGCGGCATTATTCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAG‑AAAAAAAATCActct                                                                           <  1:2613047/93‑1 (MQ=255)
              aGGAAGTCGTTCTGACTGCTGTTGATGTGGCGGCATTATTCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAG‑AAAAAAAATCActct                                                                           <  1:3841404/93‑1 (MQ=255)
               ggAAGTCGTTCTGACTGCTGTTGATGTGGCGGCATTATTCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAG‑AAAAAAAATCACTCTg                                                                          >  2:4794263/1‑93 (MQ=255)
               ggAAGTCGTTCTGACTGCTGTTGATGTGGCGGCATTATTCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAG‑AAAAAAAATCACTCTg                                                                          >  1:784063/1‑93 (MQ=255)
                 aaGTCGTTCTGACTGCTGTTGATGTGGCGGCATTATTCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAG‑AAAAAAAATCCCTCTGCa                                                                        <  2:3486418/93‑1 (MQ=255)
                  aGTCGTTCTGACTGCTGTTGATGTGGCGGCATTATTCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAG‑AAAAAAAATCACTCTGCAt                                                                       <  2:6450213/93‑1 (MQ=255)
                  aGTCGTTCTGACTGCTGTTGATGTGGCGGCATTATTCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAG‑AAAAAAAATCACTCTGCAt                                                                       <  2:6361280/93‑1 (MQ=255)
                  aGTCGTTCTGACTGCTGTTGATGTGGCGGCATTATTCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAG‑AAAAAAAATCACTCTGCAt                                                                       >  1:6091251/1‑93 (MQ=255)
                   gTCGTTCTGACTGCTGTTGATGTGGCGGCATTATTCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAG‑AAAAAAAATCACTCTGCATg                                                                      >  2:6200167/1‑93 (MQ=255)
                   gTCGTTCTGACTGCTGTTGATGTGGCGGCATTATTCCGCCGCGTCAGCGAAGCGCGCACCGCGCAGTTGGCAG‑GAAAAAAATCACTCTGCAtt                                                                      >  1:3943815/1‑92 (MQ=255)
                     cGTTCTGACTGCTGTTGATGTGGCGGCATTATTCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAG‑AAAAAAAATCACTCTGCATGtt                                                                    <  1:550635/93‑1 (MQ=255)
                       ttCTGACTGCTGTTGATGTGGCGGCATTATTCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAAAATCACTCTGCATGTTa                                                                   >  2:3060525/1‑93 (MQ=255)
                       ttCTGACTGCTGTTGATGTGGCGGCATTATTCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAG‑AAAAAAAATCACTCTGCATGTTAc                                                                  >  1:4648702/1‑93 (MQ=255)
                       ttCTGACTGCTGTTGATGTGGCGGCATTATTCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAG‑AAAAAAAATCACTCTGCATGTTAc                                                                  >  2:2234737/1‑93 (MQ=255)
                         cTGACTGCTGTTGATGTGGCGGCATTATTCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAG‑AAAAAAAATCACTCTGCATGTTACGc                                                                >  2:2381626/1‑93 (MQ=255)
                          tGACTGCTGTTGATGTGGCGGCATTATTCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAG‑AAAAAAAATCACTCTGCATGTTACGcc                                                               <  1:3311749/93‑1 (MQ=255)
                          tGACTGCTGTTGATGTGGCGGCATTATTCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAG‑AAAAAAAATCACTCTGCATGTTACGcc                                                               >  2:3984966/1‑93 (MQ=255)
                           gACTTCTGTTGATGTGGCGGCATTATTCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAG‑AAAAAAAATCACTCTGCATGTTACGccc                                                              <  2:1595772/93‑1 (MQ=255)
                              tgctgTTGATGTGGCGGCGTTATTCCGCAGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAG‑AAAAAAAATCACTCTGCTTGTTACGCCCAca                                                           >  2:4152265/1‑92 (MQ=255)
                              tgctgTTGATGTGGCGGCATTATTCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAG‑AAAAAAAATCACTCTGCATGTTACGCCCAcc                                                           <  1:4112433/93‑1 (MQ=255)
                               gctgTTGATGTGGCGGCATTATTCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAG‑AAAAAAAATCACTCTGCATGTTACGCCCACCg                                                          <  2:2531551/93‑1 (MQ=255)
                                 tgTTGATGTGGCGGCATTATTCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAG‑AAAAAAAATCACTCTGCATGTTACGCCCACCGAg                                                        >  1:3562752/1‑93 (MQ=255)
                                  gTTGATGTGGCGGCATTATTCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAG‑AAAAAAAATCACTCTGCATGTTACGCCCACCGAgg                                                       <  2:6117735/93‑1 (MQ=255)
                                    tGATGTGGCGGCATTATTCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAG‑AAAAAAAATCACTCTGCATGTTACGCCCACCGAGGtt                                                     <  1:1931439/93‑1 (MQ=255)
                                     gATGTGGCGGCATTATTCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAG‑AAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTa                                                    <  1:4448077/93‑1 (MQ=255)
                                       tgtgGCGGCATTATTCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAG‑AAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAAc                                                  <  1:6174278/93‑1 (MQ=255)
                                         tgGCGGCATTATTCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAG‑AAAAAAAATCGCTCTGCATGTTACGCCCACCGAGGTTAACGt                                                <  1:3426612/93‑1 (MQ=255)
                                         tgGCGGCATTATTCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAG‑AAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAACGt                                                >  2:4466855/1‑93 (MQ=255)
                                           gcggcATTATTCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAG‑AAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAACGTtg                                              <  2:1995989/93‑1 (MQ=255)
                                            cggcATTATTCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAG‑AAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAACGTtgc                                             <  1:2228516/93‑1 (MQ=255)
                                            cggcATTATTCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAG‑AAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAACGTtgc                                             <  1:3147805/93‑1 (MQ=255)
                                            cggcATTATTCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAG‑AAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAACGTtgc                                             <  2:5009223/93‑1 (MQ=255)
                                            cggcATTATTCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAG‑AAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAACGTtgc                                             <  1:3189163/93‑1 (MQ=255)
                                             ggcATTATTCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAG‑AAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAACGTtgct                                            >  1:5073112/1‑93 (MQ=255)
                                              gcATTATTCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAG‑AAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAACGTtgctg                                           >  1:2892139/1‑93 (MQ=255)
                                               cATTATTCCGCCGCGTCAGCGAAGTGCGCACCGTGCAGTTGGCAG‑AAAAAAAATTACTCTGCATGCTACGCCCACCGAGGTTAACGAtgctgc                                          >  2:3805231/1‑93 (MQ=255)
                                               cATTATTCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAG‑AAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAACGTtgctgc                                          <  2:3258644/93‑1 (MQ=255)
                                                attattCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAG‑AAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAACGTTgctgct                                         >  1:1487606/1‑93 (MQ=255)
                                                 ttattCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAG‑AAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAACGTTGctgctg                                        <  1:1730884/93‑1 (MQ=255)
                                                  tattCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAG‑AAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAACGTTGCTGCTGa                                       >  2:5397217/1‑93 (MQ=255)
                                                  tattCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAG‑AAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAACGTTGCTGCTGa                                       <  2:2760028/93‑1 (MQ=255)
                                                   attCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAG‑AAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAACGTTGCTGCTGaa                                      <  2:1713426/93‑1 (MQ=255)
                                                   attCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAG‑AAAAAAAATCACTCTGCATGTTACGCCCACCGAGGATAACGTTGCTGCTGaa                                      >  2:3030051/1‑93 (MQ=255)
                                                        gccgcGTCAGCGAAGCGCGCACCGTGCAGTTGGCAG‑AAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAACGTTGCTGCTGAACCGGc                                 >  2:2166397/1‑93 (MQ=255)
                                                           gcgTCAGCGAAGCGCGCACCGTGCAGTTGGCAG‑AAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAACGTTGCTGCTGAACCGGCGtt                              >  1:3729750/1‑93 (MQ=255)
                                                           gcgTCAGCGAAGCGCGCACCGTGCAGTTGGCAG‑AAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAACGTTGCTGCTGAACCGGCGtt                              >  1:4706824/1‑93 (MQ=255)
                                                             gTCAGCGAAGCGCGCACCGTGCAGTTGGCAG‑AAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAACGTTGCTGCTGAACCGGCGTTAc                            >  2:798125/1‑93 (MQ=255)
                                                             gTCAGCGAAGCGCGCACCGTGCAGTTGGCAG‑AAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAACGTTGCTGCTGAACCGGCGTTAc                            >  1:4015542/1‑93 (MQ=255)
                                                             gTCAGCGAAGCGCGCACCGTGCAGTTGGCAG‑AAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAACGTTGCTGCTGAACCGGCGTTAc                            <  1:5284531/93‑1 (MQ=255)
                                                              tCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAACGTTGCTGCTGAACCGGCGTTAc                            >  1:3985589/1‑93 (MQ=255)
                                                              tCAGCGAAGCGCGCACCGTGCAGTTGGCAG‑AAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAACGTTGCTGCTGAACCGGCGTTACt                           <  2:2672431/93‑1 (MQ=255)
                                                              tCAGCGAAGCGCGCACCGTGCAGTTGGCAG‑AAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAACGTTGCTGCTGAACCGGCGTTACt                           >  2:2497912/1‑93 (MQ=255)
                                                               cAGCGAAGCGCGCACCGTGCAGTTGGCAG‑AAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAACGTTGCTGCTGCACCGGCGTTACTg                          <  2:6207549/93‑1 (MQ=255)
                                                                aGCGAAGCGCGCACCGTGCAGTTGGCAG‑AAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAACGTTGCTGCTGAACCGGCGTTACTgg                         <  2:5817428/93‑1 (MQ=255)
                                                                aGCGAAGCGCGCACCGTGCAGTTGGCAG‑AAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAACGTTGCTGCTGAACCGGCGTTACTgg                         <  2:1849325/93‑1 (MQ=255)
                                                                aGCGAAGCGCGCACCGTGCAGTTGGCAG‑AAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAACGTTGCTGCTGAACCGGCGTTACTgg                         >  1:5915226/1‑93 (MQ=255)
                                                                 gCGAAGCGCGCACCGTGCAGTTGGCAG‑AAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAACGTTGCTGCTGAACCGGCGTTACTGGa                        >  2:2034477/1‑93 (MQ=255)
                                                                 gCGAAGCGCGCACCGTGCAGTTGGCAG‑AAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAACGTTGCTGCTGAACCGGCGTTACTGGa                        <  2:525955/93‑1 (MQ=255)
                                                                 gCGAAGCGCGCACCGTGCAGTTGGCAG‑AAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAACGTTGCTGCTGAACCGGCGTTACTGGa                        <  2:3126892/93‑1 (MQ=255)
                                                                   gAAGCGCGCACCGTGCAGTTGGCAG‑AAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAACGTTGCTGCTGAACCGGCGTTACTGGAGc                      >  1:53588/1‑93 (MQ=255)
                                                                    aaGCGCGCACCGTGCAGTTGGCAG‑AAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAACGTTGCTGCTGAACCGGCGTTACTGGAGCa                     <  2:3317426/93‑1 (MQ=255)
                                                                    aaGCGCGCACCGTGCAGTTGGCAG‑AAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAACGTTGCTGCTGAACCGGCGTTACTGGAGCa                     <  2:2584750/93‑1 (MQ=255)
                                                                     aGCGCGCCCCGTGCAGTTGGCAG‑AAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAACGTTGCTGCTGAACCGGCGTTACTGGAGCAg                    >  2:2792793/1‑93 (MQ=255)
                                                                          gcACCGTGCAGTTGGCAG‑AAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAACGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCt               >  1:3925/1‑93 (MQ=255)
                                                                          gcACCGTGCAGTTGGCAG‑AAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAACGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCt               >  2:1371614/1‑93 (MQ=255)
                                                                          gcACCGTGCAGTTGGCAG‑AAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAACGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCt               >  2:5636147/1‑93 (MQ=255)
                                                                          gcACCGTGCAGTTGGCAG‑AAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAACGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCt               >  2:5463507/1‑93 (MQ=255)
                                                                           cACCGTGCAGTTGGCAG‑AAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAACGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTg              <  1:5172475/93‑1 (MQ=255)
                                                                            aCCGTGCAGTTGGCAG‑AAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAACGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTgg             >  2:1666422/1‑93 (MQ=255)
                                                                             ccGTGCAGTTGGCAG‑AAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAACGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTggg            <  2:5127150/93‑1 (MQ=255)
                                                                             ccGTGCAGTTGGCAG‑AAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAACGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTggg            >  1:5676336/1‑93 (MQ=255)
                                                                             ccGTGCAGTTGGCAG‑AAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAACGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTggg            >  1:923667/1‑93 (MQ=255)
                                                                              cGTGCAGTTGGCAG‑AAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAACGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGa           >  2:4661039/1‑93 (MQ=255)
                                                                              cGTGCAGTTGGCAG‑AAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAACGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGa           >  2:3555636/1‑93 (MQ=255)
                                                                                  cAGTTGGCAG‑AAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAACGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGAAAtt       >  1:100340/1‑93 (MQ=255)
                                                                                  cAGTTGGCAG‑AAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAACGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGAAAtt       <  1:666091/93‑1 (MQ=255)
                                                                                   aGTTGGCAG‑AAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAACGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGACAttt      >  2:6439283/1‑93 (MQ=255)
                                                                                   aGTTGGCAG‑AAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAACGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGAAAttt      >  1:2364677/1‑93 (MQ=255)
                                                                                       ggCAG‑AAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAACGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGAAATTTACTg  >  1:4968777/1‑93 (MQ=255)
                                                                                            |                                                                                        
ACTGGAGAATCGTCAGGAAGTCGTTCTGACTGCTGTTGATGTGGCGGCATTATTCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAACGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGAAATTTACTG  >  NC_000913/4635212‑4635392

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AATCGTCAGGAAGTCGTTCTGACTGCTGTTGATGTGGCGGCATTATTCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAACGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGAAATTTACTGGATAACGCCATC  >  NC_000913/4635219‑4635404
                                                                                             |                                                                                            
AATCGTCAGGAAGTCGTTCTGACTGCTGTTGATGTGGCGGCATTATTCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAAAT                                                                                              >  SRR4302537.4571419/1‑94 (MQ=60)
 ATCGTCAGGAAGTCGTTCTGACTGCTGTTGATGTGGCGGCATTATTCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAAAAT                                                                                             >  SRR4302537.4810960/1‑94 (MQ=60)
  TCGTCAGGAAGTCGTTCTGACTGCTGTTGATGTGGCGGCATTATTCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAG‑AAAAAAAATCA                                                                                           <  SRR4302537.2182460/94‑1 (MQ=60)
  TCGTCAGGAAGTCGTTCTGACTGCTGTTGATGTGGCGGCATTATTCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAG‑AAAAAAAATCA                                                                                           <  SRR4302537.1711947/94‑1 (MQ=60)
  TCGTCAGGAAGTCGTTCTGACTGCTGTTGATGTGGCGGCATTATTCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAG‑AAAAAAAATCA                                                                                           <  SRR4302537.5917213/94‑1 (MQ=60)
   CGTCAGGAAGTCGTTCTGACTGCTGTTGATGTGGCGGCATTATTCCGGCGCGTCCGCGAAGCGCGCACCGTGGAGTTGGCAG‑AAAAAAAATCAC                                                                                          >  SRR4302537.1967986/1‑94 (MQ=60)
   CGTCAGGAAGTCGTTCTGACTGCTGTTGATGTGGCGGCATTATTCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAG‑AAAAAAAATCAC                                                                                          >  SRR4302537.3282887/1‑94 (MQ=60)
    GTCAGGAAGTCGTTCTGACTGCTGTTGATGTGGCGGCATTATTCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAG‑AAAAAAAATCACT                                                                                         <  SRR4302537.1296744/94‑1 (MQ=60)
    GTCAGGAAGTCGTTCTGACTGCTGTTGATGTGGCGGCATTATTCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAG‑AAAAAAAATCACT                                                                                         >  SRR4302537.3187498/1‑94 (MQ=60)
     TCAGGAAGTCGTTCTGACTGCTGTTGATGTGGCGGCATTATTCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAG‑AAAAAAAATCACTC                                                                                        <  SRR4302537.2937853/94‑1 (MQ=60)
      CAGGAAGTCGTTCTGACTGCTGTTGATGTGGCGGCATTATTCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAG‑AAAAAAAATCACTCT                                                                                       >  SRR4302537.1678203/1‑94 (MQ=60)
      CAGGAAGTCGTTCTGACTGCTGTTGATGTGGCGGCATTATTCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAG‑AAAAAAAATCACTCT                                                                                       <  SRR4302537.2120467/94‑1 (MQ=60)
      CAGGAAGTCGTTCTGACTGCTGTTGATGTGGCGGCATTATTCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAG‑AAAAAAAATCACTCT                                                                                       >  SRR4302537.3360237/1‑94 (MQ=60)
      CAGGAAGTCGTTCTGACTGCTGTTGATGTGGCGGCATTATTCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAG‑AAAAAAAATCACTCT                                                                                       <  SRR4302537.2820034/94‑1 (MQ=60)
       AGGAAGTCGTTCTGACTGCTGTTGATGTGGCGGCATTATTCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAG‑AAAAAAAATCACTCTG                                                                                      <  SRR4302537.3868311/94‑1 (MQ=60)
       AGGAAGTCGTTCTGACTGCTGTTGATGTGGCGGCATTATTCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAG‑AAAAAAAATCACTCTG                                                                                      >  SRR4302537.1189302/1‑94 (MQ=60)
       AGGAAGTCGTTCTGACTGCTGTTGATGTGGCGGCATTATTCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAG‑AAAAAAAATCACTCTG                                                                                      <  SRR4302537.2631327/94‑1 (MQ=60)
       AGGAAGTCGTTCTGACTGCTGTTGATGTGGCGGCATTATTCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAG‑AAAAAAAATCACTCTG                                                                                      <  SRR4302537.5493860/94‑1 (MQ=60)
       AGGAAGTCGTTCTGACTGCTGTTGATGTGGCGGCATTATTCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAG‑AAAAAAAATCACTCTG                                                                                      <  SRR4302537.6445958/94‑1 (MQ=60)
       AGGAAGTCGTTCTGACTGCTGTTGATGTGGCGGCATTATTCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAG‑AAAAAAAATCACTCTG                                                                                      <  SRR4302537.690608/94‑1 (MQ=60)
       AGGAAGTCGTTCTGACTGCTGTTGATGTGGCGGCATTATTCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAG‑AAAAAAAATCACTCTG                                                                                      >  SRR4302537.789443/1‑94 (MQ=60)
         GAAGTCGTTCTGACTGCTGTTGATGTGGCGGCATTATTCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAG‑AAAAAAAATCACTCTGCA                                                                                    >  SRR4302537.2847274/1‑94 (MQ=60)
          AAGTCGTTCTGACTGCTGTTGATGTGGCGGCATTATTCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAG‑AAAAAAAATCACTCTGCAT                                                                                   >  SRR4302537.6133102/1‑94 (MQ=60)
           AGTCGTTCTGACTGCTGTTGATGTGGCGGCATTATTCCGCCGCGTCAGCGAAGCGCGCACCGCGCAGTTGGCAG‑GAAAAAAATCACTCTGCATT                                                                                  >  SRR4302537.3971399/1‑94 (MQ=60)
           AGGCGTTCTGACTGCTGTTGATGTGGCGGCATTATTCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTAGCAG‑AAAAAAAATCTCTCTGCATG                                                                                  <  SRR4302537.1718415/94‑1 (MQ=60)
              CGTTCTGACTGCTGTTGATGTGGCGGCATTATTCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAG‑AAAAAAAATCACTCTGCATGTTA                                                                               <  SRR4302537.554424/94‑1 (MQ=60)
              CGTTCTGACTGCTGTTGATGTGGCGGCATTATTCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAG‑AAAAAAAATCACTCTGCATGTTA                                                                               >  SRR4302537.4027034/1‑94 (MQ=60)
              CGTTCTGACTGCTGTTGATGTGGCGGCATTATTCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAG‑AAAAAAAATCACTCTGCATGTTA                                                                               <  SRR4302537.5935478/94‑1 (MQ=60)
              CGTTCTGACTGCTGTTGATGTGGCGGCATTATTCCGACGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAG‑AAAAAAAATCACTCTGCATGTTA                                                                               <  SRR4302537.6337088/94‑1 (MQ=60)
               GTTCTGACTGCTGTTGATGTGGCGGCATTATTCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAG‑AAAAAAAATCACTCTGCATGTTAC                                                                              >  SRR4302537.4680840/1‑94 (MQ=60)
               GTTCTGACTGCTGTTGATGTGGCGGCATTATTCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAG‑AAAAAAAATCACTCTGCATGTTAC                                                                              <  SRR4302537.5698446/94‑1 (MQ=60)
               GTTCTGACTGCTGTTGATGTGGCGGCATTATTCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAG‑AAAAAAAAGCACTCTGCATGTTAC                                                                              >  SRR4302537.5276286/1‑94 (MQ=60)
                TTCTGACTGCTGTTGATGTGGCGGCATTATTCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCCG‑AAAAAAAATCACTCTGCATGTTACG                                                                             >  SRR4302537.3958115/1‑94 (MQ=60)
                TTCTGACTGCTGTTGATGTGGCGGCATTATTCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAG‑AAAAAAAATCACTCTGCATGTTACG                                                                             <  SRR4302537.4782584/94‑1 (MQ=60)
                   TGACTGCTGTTGATGTGGCGGCATTATTCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAG‑AAAAAAAATCACTCTGCATGTTACGCCC                                                                          >  SRR4302537.6136286/1‑94 (MQ=60)
                   TGACTGCTGTTGATGTGGCGGCATTATTCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAG‑AAAAAAAATCACTCTGCATGTTACGCCC                                                                          <  SRR4302537.3335179/94‑1 (MQ=60)
                   TGACTGCTGTTGATGTGGCGGCATTATTCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAG‑AAAAAAAATCACTCTGCATGTTACGCCC                                                                          <  SRR4302537.2114629/94‑1 (MQ=60)
                   TGACTGCTGTTGATGTGGCGGCATTATTCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAG‑AAAAAAAATCACTCTGCATGTTACGCCC                                                                          >  SRR4302537.1857317/1‑94 (MQ=60)
                   TGACTGCTGTTGATGTGGCGGCATTATTCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAG‑AAAAAAAATCACTCTGCATGTTACGCCC                                                                          <  SRR4302537.5366614/94‑1 (MQ=60)
                       TGCTGTTGATGTGGCGGCATTATTCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAG‑AAAAAAAATCACTCTGCATGTTACGCCCACCG                                                                      <  SRR4302537.4141161/94‑1 (MQ=60)
                        GCTGTTGATGTGGCGGCATTATTCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAG‑AAAAAAAATCACTCTGCATGTTACGCCCACCGA                                                                     >  SRR4302537.5805404/1‑94 (MQ=60)
                         CTGTTGATGTGGCGGCATTATTCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAG‑AAAAAAAATCACTCTGCATGTTACGCCCACCGAG                                                                    >  SRR4302537.3587881/1‑94 (MQ=60)
                          TGTTGATGTGGCGGCATTATTCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAG‑AAAAAAAATCACTCTGCATGTTACGCCCACCGAGG                                                                   <  SRR4302537.4733705/94‑1 (MQ=60)
                          TGTTGATGTGGCGGCATTATTCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAG‑AAAAAAAATCACTCTGCATGTTACGCCCACCGAGG                                                                   >  SRR4302537.2343894/1‑94 (MQ=60)
                           GTTGATGTGGCGGCATTATTCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAG‑AAAAAAAATCACTCTGCATGTTACGCCCACCGAGGT                                                                  <  SRR4302537.5225911/94‑1 (MQ=60)
                           GTTGATGTGGCGGCATTATTCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAG‑AAAAAAAATCACTCTGCATGTTACGCCCACCGAGGT                                                                  <  SRR4302537.5464550/94‑1 (MQ=60)
                            TTGATGTGGCGGCATTATTCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAG‑AAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTT                                                                 >  SRR4302537.3384633/1‑94 (MQ=60)
                            TTGATGTGGCGGCATTATTCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAG‑AAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTT                                                                 <  SRR4302537.5102335/94‑1 (MQ=60)
                             TGATGTGGCGGCATTATTCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAG‑AAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTA                                                                <  SRR4302537.1944875/94‑1 (MQ=60)
                             TGATGTGGCGGCATTATTCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAG‑AAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTA                                                                <  SRR4302537.3199766/94‑1 (MQ=60)
                             TGATGTGGCGGCATTATTCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAG‑AAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTA                                                                >  SRR4302537.5848816/1‑94 (MQ=60)
                              GATGTGGCGGCATTATTCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAG‑AAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAA                                                               <  SRR4302537.4478795/94‑1 (MQ=60)
                                TGTGGCGGCATTATTCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAG‑AAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAACG                                                             <  SRR4302537.6216800/94‑1 (MQ=60)
                                  TGGCGGCATTATTCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAG‑AAAAAAAATCGCTCTGCATGTTACGCCCACCGAGGTTAACGTT                                                           <  SRR4302537.3450867/94‑1 (MQ=60)
                                  TGGCGGCATTATTCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAG‑AAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAACGTT                                                           <  SRR4302537.5993671/94‑1 (MQ=60)
                                     CGGCATTATTCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAG‑AAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAACGTTGCT                                                        <  SRR4302537.3211593/94‑1 (MQ=60)
                                     CGGCATTATTCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAG‑AAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAACGTTGCT                                                        <  SRR4302537.3169885/94‑1 (MQ=60)
                                     CGGCATTATTCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAG‑AAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAACGTTGCT                                                        >  SRR4302537.1519380/1‑94 (MQ=60)
                                     CGGCATTATTCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAG‑AAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAACGTTGCT                                                        >  SRR4302537.5108039/1‑94 (MQ=60)
                                     CGGCATTATTCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAG‑AAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAACGTTGCT                                                        <  SRR4302537.2244230/94‑1 (MQ=60)
                                      GGCATTATTCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAG‑AAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAACGTTGCTG                                                       >  SRR4302537.2912442/1‑94 (MQ=60)
                                        CATTATTCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAG‑AAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAACGTTGCTGCT                                                     <  SRR4302537.6147700/94‑1 (MQ=60)
                                        CATTATTCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAG‑AAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAACGTTGCTGCT                                                     >  SRR4302537.5527603/1‑94 (MQ=60)
                                        CATTATTCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAG‑AAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAACGTTGCTGCT                                                     >  SRR4302537.1497957/1‑94 (MQ=60)
                                        CATTATTCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAG‑AAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAACGTTGCTGCT                                                     <  SRR4302537.3252849/94‑1 (MQ=60)
                                         CTTATTCCGCCGCGTCAGCGAAGCGCGCACCGTGCGGTTGGCAGAAAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAACGTTGCTGCT                                                     <  SRR4302537.741872/94‑1 (MQ=60)
                                          TTATTCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAG‑AAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAACGTTGCTGCTGA                                                   <  SRR4302537.1742965/94‑1 (MQ=60)
                                           TATTCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAG‑AAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAACGTTGCTGCTGAA                                                  <  SRR4302537.3456446/94‑1 (MQ=60)
                                              TCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAG‑AAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAACGTTGCTGCTGAACCG                                               <  SRR4302537.402280/94‑1 (MQ=60)
                                                CGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAG‑AAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAACGTTGCTGCTGAACCGGC                                             >  SRR4302537.3696000/1‑94 (MQ=60)
                                                 GCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAG‑AAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAACGTTGCTGCTGAACCGGCG                                            >  SRR4302537.5495341/1‑94 (MQ=60)
                                                 GCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAG‑AAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAACGTTGCTGCTGAACCGGCG                                            >  SRR4302537.4399007/1‑94 (MQ=60)
                                                  CCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAG‑AAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAACGTTGCTGCTGAACCGGCGT                                           >  SRR4302537.3642308/1‑94 (MQ=60)
                                                   CGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAG‑AAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAACGTTGCTGCTGAACCGGCGTT                                          >  SRR4302537.3755952/1‑94 (MQ=60)
                                                   CGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAG‑AAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAACGTTGCTGCTGAACCGGCGTT                                          >  SRR4302537.4739388/1‑94 (MQ=60)
                                                    GCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAG‑AAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAACGTTGCTGCTGAACCGGCGTTA                                         >  SRR4302537.6454378/1‑94 (MQ=60)
                                                    GCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAG‑AAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAACGTTGCTGCTGAACCGGCGTTA                                         <  SRR4302537.5072545/94‑1 (MQ=60)
                                                     CGTCAGCGAAGCGCGCACCGTGCAGTTGGCAG‑AAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAACGTTGCTGCTGAACCGGCGTTAC                                        >  SRR4302537.4043658/1‑94 (MQ=60)
                                                     CGTCAGCGAAGCGCGCACCGTGCAGTTGGCAG‑AAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAACGTTGCTGCTGAACCGGCGTTAC                                        <  SRR4302537.2002776/94‑1 (MQ=60)
                                                     CGTCAGCGAAGCGCGCACCGTGCAGTTGGCAG‑AAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAACGTTGCTGCTGAACCGGCGTTAC                                        <  SRR4302537.1962330/94‑1 (MQ=60)
                                                      GTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAACGTTGCTGCTGAACCGGCGTTAC                                        >  SRR4302537.4013494/1‑94 (MQ=60)
                                                      GTCAGCGAAGCGCGCACCGTGCAGTTGGCAG‑AAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAACGTTGCTGCTGAACCGGCGTTACT                                       >  SRR4302537.4468199/1‑94 (MQ=60)
                                                      GTCAGCGAAGCGCGCACCGTGCAGTTGGCAG‑AAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAACGTTGCTGCTGAACCGGCGTTACT                                       >  SRR4302537.219394/1‑94 (MQ=60)
                                                      GTCAGCGAAGCGCGCACCGTGCAGTTGGCAG‑AAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAACGTTGCTGCTGAACCGGCGTTACT                                       >  SRR4302537.2804362/1‑94 (MQ=60)
                                                      GTCAGCGAAGCGCGCACCGTGCAGTTGGCAG‑AAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAACGTTGCTGCTGAACCGGCGTTACT                                       <  SRR4302537.2769990/94‑1 (MQ=60)
                                                      GTCAGCGAAGCGCGCACCGTGCAGTTGGCAG‑AAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAACGTTGCTGCTGAACCGGCGTTACT                                       <  SRR4302537.5320871/94‑1 (MQ=60)
                                                       TCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAACGTTGCTGCTGAACCGGCGTTACT                                       <  SRR4302537.3346898/94‑1 (MQ=60)
                                                       TCAGCGAAGCGCGCACCGTGCAGTTGGCAG‑AAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAACGTTGCTGCTGAACCGGCGTTACTG                                      <  SRR4302537.3841987/94‑1 (MQ=60)
                                                        CATCGAACCGCGCACCGTGCAGTTGGCAG‑AAAAAAAATCACTCTGCATGTTTCGCCCACCGAGGTTAACGTTGCTGCTGAACCGGCGTTACTGG                                     <  SRR4302537.4050982/94‑1 (MQ=60)
                                                        CAGCGAAGCGCGCACCGTGCAGTTGGCAG‑AAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAACGTTGCTGCTGAACCGGCGTTACTGG                                     >  SRR4302537.5955819/1‑94 (MQ=60)
                                                         AGCGAAGCGCGCACCGTGCAGTTGGCAG‑AAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAACGTTGCTGCTGAACCGGCGTTACTGGA                                    >  SRR4302537.2080401/1‑94 (MQ=60)
                                                           CGAAGCGCGCACCGTGCAGTTGGCAG‑AAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAACGTTGCTGCTGAACCGGCGTTACTGGAGC                                  >  SRR4302537.53982/1‑94 (MQ=60)
                                                           CGAAGCGCGCACCGTGCAGTTGGCAG‑AAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAACGTTGCTGCTGAACCGGCGTTACTGGAGC                                  <  SRR4302537.6454815/94‑1 (MQ=60)
                                                            GAAGCGCGCACCGTGCAGTTGGCAG‑AAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAACGTTGCTGCTGAACCGGCGTTACTGGAGCG                                 >  SRR4302537.1373602/1‑94 (MQ=60)
                                                            GAAGCGCGCACCGTGCAGTTGGCAG‑AAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAACGTTGCTGCTGAACCGGCGTTACTGGAGCA                                 >  SRR4302537.3530198/1‑94 (MQ=60)
                                                             AAGCGCGCACCGTGCAGTTGGCAG‑AAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAACGTTGCTGCTGAACCGGCGTTACTGGAGCAG                                >  SRR4302537.456736/1‑94 (MQ=60)
                                                             AAGCGCGCACCGTGCAGTTGGCAG‑AAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAACGTTGCTGCTGAACCGGCGTTACTGGAGCAG                                <  SRR4302537.546733/94‑1 (MQ=60)
                                                             AAGCGCGCACCGTGCAGTTGGCAG‑AAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAACGTTGCTGCTGAACCGGCGTTACTGGAGCAG                                <  SRR4302537.3020739/94‑1 (MQ=60)
                                                              AGCGCGCACCGTGCAGTTGGCAGAAAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAACGTTGCTGCTGAACCGGCGTTACTGGAGCAG                                <  SRR4302537.5160500/94‑1 (MQ=60)
                                                              AGCGCGCACCGTGCAGTTGGCAG‑AAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAACGTTGCTGCTGAACCGGCGTTACTGGAGCAGG                               <  SRR4302537.1894244/94‑1 (MQ=60)
                                                              AGCGCGCACCGTGCAGTTGGCAG‑AAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAACGTTGCTGCTGAACCGGCGTTACTGGAGCAGG                               <  SRR4302537.2607806/94‑1 (MQ=60)
                                                                CGCGCACCGTGCAGTTGGCAG‑AAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAACGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCG                             <  SRR4302537.3007189/94‑1 (MQ=60)
                                                                  CGCACCGTGCAGTTGGCAG‑AAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAACGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCT                           >  SRR4302537.3959/1‑94 (MQ=60)
                                                                  CGCACCGTGCAGTTGGCAG‑AAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAACGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCT                           <  SRR4302537.3945179/94‑1 (MQ=60)
                                                                    CACCGTGCAGTTGGCAG‑AAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAACGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGG                         >  SRR4302537.2385915/1‑94 (MQ=60)
                                                                    CACCGTGCAGTTGGCAG‑AAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAACGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGG                         <  SRR4302537.5208082/94‑1 (MQ=60)
                                                                    CACCGTGCAGTGGGCAG‑AAAAAAGATCACTCTGCATGTTACGCCCACCGAGGTTAACGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGG                         <  SRR4302537.1999683/94‑1 (MQ=60)
                                                                     ACCGTGCAGTTGGCAG‑AAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAACGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGG                        >  SRR4302537.930042/1‑94 (MQ=60)
                                                                     ACCGTGCAGTTGGCAG‑AAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAACGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGG                        >  SRR4302537.5715174/1‑94 (MQ=60)
                                                                        GTGCAGTTGGCAG‑AAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAACGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGAAA                     >  SRR4302537.134880/1‑94 (MQ=60)
                                                                          GCAGTTGGCAG‑AAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAACGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGAAATT                   >  SRR4302537.101062/1‑94 (MQ=60)
                                                                           CAGTTGGCAG‑AAAAAAAATCACTCTGCATGTTACGGCCACCGAGGTTAACGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGAAATTT                  >  SRR4302537.5430474/1‑94 (MQ=60)
                                                                           CAGTTGGCAG‑AAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAACGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGAAATTT                  >  SRR4302537.2381350/1‑94 (MQ=60)
                                                                           CAGTTGGCAG‑AAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAACGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGAAATTT                  <  SRR4302537.670631/94‑1 (MQ=60)
                                                                            AGTTGGCAG‑AAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAACGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGAAATTTA                 >  SRR4302537.5926196/1‑94 (MQ=60)
                                                                             GTTGGCAG‑AAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAACGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGAAATTTAC                <  SRR4302537.2242715/94‑1 (MQ=60)
                                                                              TTGGCAG‑AAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAACGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGAAATTTACT               >  SRR4302537.3991926/1‑94 (MQ=60)
                                                                               TGGCAG‑AAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAACGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGAAATTTACTG              >  SRR4302537.5003019/1‑94 (MQ=60)
                                                                               TGGCAG‑AAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAACGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGAAATTTACTG              <  SRR4302537.799763/94‑1 (MQ=60)
                                                                                GGCAG‑AAAAAAAATCACTCTGCATGTTCCGCCCCCCGAGGTTAACGTTGCTGCTGAACCGGCGTTACTGGAGCAGCCGCTGGGAAATTTACTGT             >  SRR4302537.2227772/1‑94 (MQ=60)
                                                                                GGCAG‑AAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAACGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGAAATTTACTGG             <  SRR4302537.3741174/94‑1 (MQ=60)
                                                                                 GCAG‑AAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAACGTTTCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGTAAATTTACTGGA            >  SRR4302537.5203337/1‑94 (MQ=60)
                                                                                 GCAG‑AAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAACGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGAAATTTACTGGA            <  SRR4302537.3717294/94‑1 (MQ=60)
                                                                                  CAG‑AAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAACGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGAAATTTACTGGAT           <  SRR4302537.1743248/94‑1 (MQ=60)
                                                                                    agAAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAACGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGAAATTTACTGGATA          >  SRR4302537.2154843/3‑94 (MQ=60)
                                                                                     GAAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAACGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGAAATTTACTGGATAA         >  SRR4302537.6456559/1‑94 (MQ=60)
                                                                                        AAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAACGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGAAATTTACTGGATAACGC      >  SRR4302537.1987233/1‑94 (MQ=60)
                                                                                          AAAATCACTCTGCATGTTACGCCCACCGAGGTTAACGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGAAATTTACTGGATAACGCCA    >  SRR4302537.4249654/1‑94 (MQ=60)
                                                                                          AAAATCACTCTGCATGTTACGCCCACCGAGGTTAACGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGAAATTTACTGGATAACGCCA    <  SRR4302537.2371455/94‑1 (MQ=60)
                                                                                           AAATCACTCTGCATGTTACGCCCACCGAGGTTAACGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGAAATTTACTGGATAACGCCAT   <  SRR4302537.4827351/94‑1 (MQ=60)
                                                                                           AAATCACTCTGCATGTTACGCCCACCGAGGTTAACGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGAAATTTACTGGATAACGCCAT   <  SRR4302537.5110342/94‑1 (MQ=60)
                                                                                            AATCACTCTGCATGTTACGCCCACCGAGGTTAACGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGAAATTTACTGGATAACGCCATC  >  SRR4302537.5065802/1‑94 (MQ=60)
                                                                                            AATCACTCTGCATGTTACGCCCACCGAGGTTAACGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGAAATTTACTGGATAACGCCATC  <  SRR4302537.4012866/94‑1 (MQ=60)
                                                                                            AATCACTCTGCATGTTACGCCCACCGAGGTTAACGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGAAATTTACTGGATAACGCCATC  <  SRR4302537.12960/94‑1 (MQ=60)
                                                                                            AATCACTCTGCATGTTACGCCCACCGAGGTTAACGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGAAATTTACTGGATAACGCCATC  <  SRR4302537.2453600/94‑1 (MQ=60)
                                                                                            AATCACTCTGCATGTTACGCCCACCGAGGTTAACGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGAAATTTACTGGATAACGCCATC  >  SRR4302537.2049489/1‑94 (MQ=60)
                                                                                             |                                                                                            
AATCGTCAGGAAGTCGTTCTGACTGCTGTTGATGTGGCGGCATTATTCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAACGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGAAATTTACTGGATAACGCCATC  >  NC_000913/4635219‑4635404

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑