Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1061 294.1 15065038 98.6% 14854127 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,711,990 C→T L359L (CTG→TTG)  srmB → ATP‑dependent RNA helicase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,711,9900CT87.9% 650.2 / 51.6 225L359L (CTG→TTG) srmBATP‑dependent RNA helicase
Reads supporting (aligned to +/- strand):  ref base C (12/15);  new base T (96/100);  total (109/116)
Fisher's exact test for biased strand distribution p-value = 6.87e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.56e-01

Only 100 of 237 total aligned reads displayed.

GTGGCGATACTTATTTGCACCGTATCGGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCTGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTACGCCCGAAAACGCGTGCGCCAAGCGAA  >  NC_000913/2711898‑2712079
                                                                                            |                                                                                         
gTGGCGATACTTATTTGCACCGTATCGGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATctgc                                                                                           <  1:440123/93‑1 (MQ=255)
   gCGATACTTATTTGCACCGTATCGGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATctgttgc                                                                                        <  2:1793882/93‑1 (MQ=255)
   gCGATACTTATTTGCACCGTATCGGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATctgttgc                                                                                        <  1:3544831/93‑1 (MQ=255)
    cGATACTTATTTGCACCGTATCGGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATctgttgct                                                                                       >  2:4815024/1‑93 (MQ=255)
     gATACTTATTTGCACCGTATCGGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATctgttgctg                                                                                      >  2:109545/1‑93 (MQ=255)
     gATACTTATTTGCACCGTATCGGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATctgttgctg                                                                                      <  1:2957273/93‑1 (MQ=255)
     gATACTTATTTGCACCGTATCGGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATctgttgctg                                                                                      <  2:6211673/93‑1 (MQ=255)
      aTACTTATTTGCACCGTATCGGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCTGCTgg                                                                                     <  2:6775305/93‑1 (MQ=255)
        aCTTATTTGCACCGTATCGGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGTTGCTGGGt                                                                                   >  1:2352283/1‑93 (MQ=255)
        aCTTATTTGCACCGTATCGGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCTGCTGGGt                                                                                   >  1:5340834/1‑93 (MQ=255)
        aCTTATTTGCACCGTATCGGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCTGCTGGGt                                                                                   <  2:2066450/93‑1 (MQ=255)
         cTTATTTGCACCGTATCGGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGTTGCTGGGTa                                                                                  >  1:109020/1‑93 (MQ=255)
          ttATTTGCACCGTATCGGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGTTGCTGGGTaa                                                                                 >  2:1957341/1‑93 (MQ=255)
          ttATTTGCACCGTAGCGGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGTTGCTGGGTaa                                                                                 >  2:6451137/1‑93 (MQ=255)
             tttGCACCGTATCGGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGTTGCTGGGTAAAGt                                                                              <  2:1127611/93‑1 (MQ=255)
             tttGCACCGTATCGGACGTACCGCGCGCCCCGGGCGTAAAAGCCCCGCAATTTCGCTGGGTGAAGCCCATGACCAACTGGTTGTGGGTAAAGt                                                                              <  1:4146104/93‑1 (MQ=255)
              ttGCACCGTATCGGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGTTGCTGGGTAAAGTa                                                                             <  1:1898941/93‑1 (MQ=255)
                gCACCGTATCGGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGTTGCTGGGTAAAGTAgg                                                                           >  2:3246319/1‑93 (MQ=255)
                gCACCGTATCGGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGTTGCTGGGTAAAGTAgg                                                                           >  2:3861338/1‑93 (MQ=255)
                gCACCGTATCGGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGTTGCTGGGTAAAGTAgg                                                                           <  2:4298455/93‑1 (MQ=255)
                  aCCGTATCGGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGTTGCTGGGTAAAGTAGGcc                                                                         >  1:6069075/1‑93 (MQ=255)
                   ccGTATCGGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGTTGCTGGGTAAAGTAGGCCg                                                                        >  1:4923566/1‑93 (MQ=255)
                   ccGTATCGGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGTTGCTGGGTAAAGTAGGCCg                                                                        >  2:1484765/1‑93 (MQ=255)
                   ccGTATCGGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCTGCTGGGTAAAGTAGGCCg                                                                        >  1:4995063/1‑93 (MQ=255)
                    cGTATCGGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGTTGCTGGGTAAAGTAGGCCGc                                                                       >  2:561251/1‑93 (MQ=255)
                    cGTATCGGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCACATGACCATCTGCTGCTGGGTAAAGTAGGCCGc                                                                       >  2:6252544/1‑93 (MQ=255)
                      tATCGGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGTTGCTGGGTAAAGTAGGCCGCta                                                                     <  1:797818/93‑1 (MQ=255)
                      tATCGGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGTTGCTGGGTAAAGTAGGCCGCta                                                                     <  1:2029788/93‑1 (MQ=255)
                      tATCGGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGTTGCTGGGTAAAGTAGGCCGCta                                                                     >  2:2781442/1‑93 (MQ=255)
                      tATCGGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCTGCTGGGTAAAGTAGGCCGCta                                                                     <  1:7454247/93‑1 (MQ=255)
                        tCGGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGTTGCTGGGTAAAGTAGGCCGCtata                                                                   <  2:6518620/93‑1 (MQ=255)
                        tCGGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGTTGCTGGGTAAAGTAGGCCGCtata                                                                   >  1:5999405/1‑93 (MQ=255)
                          ggACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGTTGCTGGGTAAAGTAGGCCGCTATAtt                                                                 <  1:5568148/93‑1 (MQ=255)
                           gACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGTTGCTGGGTAAAGTAGGCCGCTATATTg                                                                >  2:4585433/1‑93 (MQ=255)
                           gACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGTTGCTGGGTAAAGTAGGCCGCTATATTg                                                                >  1:5248329/1‑93 (MQ=255)
                             cGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGTTGCTGGGTAAAGTAGGCCGCTATATTGaa                                                              <  2:4925599/93‑1 (MQ=255)
                              gTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGTTGCTGGGTAAAGTAGGCCGCTATATTGAag                                                             >  1:2641630/1‑93 (MQ=255)
                               tACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGTTGCTGGGTAAAGTAGGCCGCTATATTGAaga                                                            >  1:6313764/1‑93 (MQ=255)
                               tACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGTTGCTGGGTAAAGTAGGCCGCTATATTGAaga                                                            <  2:5841553/93‑1 (MQ=255)
                               tACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGTTGCTGGGTAAAGTAGGCCGCTATATTGAaga                                                            >  1:5184498/1‑93 (MQ=255)
                               tACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGCAAGCCCATGACCATCTGCTGCTGGGTAAAGTAGGCCGCTATATTGAaga                                                            <  1:7300825/93‑1 (MQ=255)
                                aCCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGTTGCTGGGTAAAGTAGGCCGCTATATTGAagag                                                           >  2:3693156/1‑93 (MQ=255)
                                  cgcgcgcgCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGTTGCTGGGTAAAGTAGGCCGCTATATTGAAGAGcc                                                         >  2:725703/1‑93 (MQ=255)
                                  cgcgcgcgCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGTTGCTGGGTAAAGTAGGCCGCTATATTGAAGAGcc                                                         <  2:5518823/93‑1 (MQ=255)
                                   gcgcgcgcCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGTTGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCa                                                        <  2:6284900/93‑1 (MQ=255)
                                   gcgcgcgcCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGTTGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCa                                                        >  1:1144410/1‑93 (MQ=255)
                                    cgcgcgcCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGTTGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCaa                                                       <  2:3652669/93‑1 (MQ=255)
                                     gcgcgcCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGTTGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAAt                                                      <  1:5786807/93‑1 (MQ=255)
                                      cgcgcCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGTTGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAAtt                                                     <  2:5358649/93‑1 (MQ=255)
                                        cgcCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGTTGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTaa                                                   <  1:7267746/93‑1 (MQ=255)
                                         gcCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGTTGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTaaa                                                  <  1:3386074/93‑1 (MQ=255)
                                           cGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGTTGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGc                                                >  1:7156743/1‑93 (MQ=255)
                                           cGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGTTGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGc                                                <  2:3722386/93‑1 (MQ=255)
                                           cGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCTGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGc                                                <  1:905990/93‑1 (MQ=255)
                                            ggTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGTTGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCt                                               >  1:6334915/1‑93 (MQ=255)
                                             gTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGTTGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTc                                              >  2:878125/1‑93 (MQ=255)
                                              tCGTAAAAGCACCGCAATTTCGCTGGGGGAAGCCCATGACCATCTGGTGCTGGGTAAAGCAGGCCGCCATATTGAAAAGCCAATTAAAGCtgg                                             >  1:3676286/1‑91 (MQ=255)
                                                gTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGTTGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTcgcg                                           >  1:6888089/1‑93 (MQ=255)
                                                gTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGTTGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTcgcg                                           >  2:2302617/1‑93 (MQ=255)
                                                 tAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGTTGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGt                                          >  1:5895575/1‑93 (MQ=255)
                                                 tAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGTTGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGt                                          >  1:4880156/1‑93 (MQ=255)
                                                 tAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGTTGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGt                                          >  1:6570296/1‑93 (MQ=255)
                                                  aaaGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGTTGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGtt                                         <  1:6733515/93‑1 (MQ=255)
                                                  aaaGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGTTGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGtt                                         >  2:527918/1‑93 (MQ=255)
                                                  aaaGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGTTGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGtt                                         <  2:826051/93‑1 (MQ=255)
                                                  aaaGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCTGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGtt                                         <  2:6858711/93‑1 (MQ=255)
                                                   aaGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGTTGCTGGGCAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTa                                        >  2:2568421/1‑93 (MQ=255)
                                                    aGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGTTGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTAt                                       <  2:5046248/93‑1 (MQ=255)
                                                    aGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCTGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTAt                                       <  1:25352/93‑1 (MQ=255)
                                                    aGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCTGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTAt                                       <  2:5259510/93‑1 (MQ=255)
                                                     ggCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGTTGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTAtt                                      <  2:7048736/93‑1 (MQ=255)
                                                      gCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGTTGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATtg                                     <  1:4226186/93‑1 (MQ=255)
                                                      gCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCTGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATtg                                     <  1:3033174/93‑1 (MQ=255)
                                                        aCCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGTTGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATtgat                                   <  1:1359821/93‑1 (MQ=255)
                                                        aCCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGTTGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATtgat                                   >  1:1459384/1‑93 (MQ=255)
                                                         ccGCAATTTCGCTGGTGGAAGCCCATGACCATCTGTTGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATtgatg                                  >  1:5131679/1‑93 (MQ=255)
                                                           gCAATTTCGCTGGTGGAAGCCCATGACCATCTGTTGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATTGATGAg                                <  2:421281/93‑1 (MQ=255)
                                                           gCAATTTCGCTGGTGGAAGCCCATGACCATCTGTTGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATTGATGAg                                <  2:3317952/93‑1 (MQ=255)
                                                            cAATTTCGCTGGTGGAAGCCCATGACCATCTGTTGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATTGATGAGt                               <  1:2819825/93‑1 (MQ=255)
                                                             aaTTTCGCTGGTGGAAGCCCATGACCATCTGCTGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATTGATGAGtt                              >  2:6640/1‑93 (MQ=255)
                                                              aTTTCGCTGGTGGAAGCCCATGACCATCTGTTGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTa                             >  2:2349801/1‑93 (MQ=255)
                                                              aTTTCGCTGGTGGAAGCCCATGACCATCTGTTGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTa                             <  1:3087028/93‑1 (MQ=255)
                                                              aTTTCGCTGGTGGAAGCCCATGACCATCTGTTGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTa                             <  1:1694348/93‑1 (MQ=255)
                                                               tttCGCTGGTGGAAGCCCATGACCATCTGTTGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTAc                            <  2:2895831/93‑1 (MQ=255)
                                                                   gCTGGTGGAAGCCCATGACCATCTGTTGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTACGccc                        <  1:1014280/93‑1 (MQ=255)
                                                                     tggtggAAGCCCATGACCATCTGTTGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTACGCCCGa                      >  2:127330/1‑93 (MQ=255)
                                                                      ggtggAAGCCCATGACCATCTGCTGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTACGCCCGaa                     >  1:6652357/1‑93 (MQ=255)
                                                                        tggAAGCCCATGACCATCTGTTGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTACGCCCGaaaa                   >  1:216878/1‑93 (MQ=255)
                                                                         ggAAGCCCATGACCATCTGTTGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTACGCCCGAAAAc                  >  2:6018401/1‑93 (MQ=255)
                                                                         ggAAGCCCATGACCATCTGTTGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTACGCCCGAAAAc                  <  1:383007/93‑1 (MQ=255)
                                                                           aaGCCCATGACCATCTGTTGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTACGCCCGAAAAcgc                <  2:3993143/93‑1 (MQ=255)
                                                                             gCCCATGACCATCTGTTGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTACGCCCGAAAACGCGt              <  1:6818215/93‑1 (MQ=255)
                                                                              cccATGACCATCTGTTGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTACGCCCGAAAACGCGTg             <  1:6052432/93‑1 (MQ=255)
                                                                               ccATGACCATCTGTTGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTACGCCCGAAAACGCGTgc            >  1:2214170/1‑93 (MQ=255)
                                                                                  tGACCATCTGTTGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTACGCCCGAAAACGCGTGCGcc         <  2:237533/93‑1 (MQ=255)
                                                                                      cATCTGCTGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTACGCCCGAAAACGCGTGCGCCAAGc     >  2:3372801/1‑93 (MQ=255)
                                                                                       aTCTGTTGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTACGCCCGAAAACGCGTGCGCCAAGCg    >  2:1683638/1‑93 (MQ=255)
                                                                                        tCTGTTGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTACGCCCGAAAACGCGTGCGCCAAGCGa   <  2:5995994/93‑1 (MQ=255)
                                                                                        tCTGCTGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTACGCCCGAAAACGCGTGCGCCAAGCGa   >  2:7322375/1‑93 (MQ=255)
                                                                                         ctgttgctgGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTACGCCCGAAAACGCGTGCGCCAAGCGaa  >  1:4240206/1‑93 (MQ=255)
                                                                                            |                                                                                         
GTGGCGATACTTATTTGCACCGTATCGGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCTGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTACGCCCGAAAACGCGTGCGCCAAGCGAA  >  NC_000913/2711898‑2712079

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AGTGGCGATACTTATTTGCACCGTATCGGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCTGCTGGGTAAAGTAGGCCGCTATATTG‑AAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTACGCCCGAAAACGCGTGCGCCAAGCGAAAAGC  >  NC_000913/2711897‑2712083
                                                                                             |                                                                                              
AGTGGCGATACTTATTTGCACCGTATCGGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGT                                                                                                >  SRR4302542.2770463/1‑94 (MQ=60)
AGTGGCGATACTTATTTGCACCGTATCGGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGT                                                                                                >  SRR4302542.2529919/1‑94 (MQ=60)
 GTGGCGATACTTATTTGCACCGTATCGGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGTT                                                                                               <  SRR4302542.1027316/94‑1 (MQ=60)
 GTGGCGATACTTATTTGCACCGTATCGGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGTT                                                                                               >  SRR4302542.6723849/1‑94 (MQ=60)
 GTGGCGATACTTATTTGCACCGTATCGGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCT                                                                                               <  SRR4302542.443939/94‑1 (MQ=60)
  TGGCGATACTTATTTGCACCGTATCGGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGTTG                                                                                              <  SRR4302542.3885965/94‑1 (MQ=60)
   GGCGATACTTATTTGCACCGTATCGGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGTTGC                                                                                             >  SRR4302542.6484575/1‑94 (MQ=60)
    GCGATACTTATTTGCACCGTATCGGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGTTGCT                                                                                            <  SRR4302542.3577940/94‑1 (MQ=60)
    GCGATACTTATTTGCACCGTATCGGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCTGCT                                                                                            <  SRR4302542.4965350/94‑1 (MQ=60)
     CGATACTTATTTGCACCGTATCGGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGTTGCTG                                                                                           >  SRR4302542.6120573/1‑94 (MQ=60)
      GATACTTATTTGCACCGTATCGGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGTTGCTGG                                                                                          <  SRR4302542.2985618/94‑1 (MQ=60)
      GATACTTATTTGCACCGTATCGGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGTTGCTGG                                                                                          >  SRR4302542.7536026/1‑94 (MQ=60)
        TACTTATTTGCACCGTATCGGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGTTGCTGGGT                                                                                        >  SRR4302542.597190/1‑94 (MQ=60)
        TACTTATTTGCACCGTATCGGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGTTGCTGGGT                                                                                        >  SRR4302542.2372482/1‑94 (MQ=60)
        TACTTATTTGCACCGTATCGGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCTGCTGGGT                                                                                        >  SRR4302542.5392721/1‑94 (MQ=60)
         ACTTATTTGCACCGTATCGGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGTTGCTGGGTA                                                                                       >  SRR4302542.109913/1‑94 (MQ=60)
          CTTATTTGCACCGTATCGGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGTTGCTGGGTAA                                                                                      >  SRR4302542.2888125/1‑94 (MQ=60)
          CTTA‑TTGCACCGTATCGGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGTTGCTGGGTAAA                                                                                     <  SRR4302542.2717804/94‑1 (MQ=60)
           TTATTTGCACCGTATCGGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGTTGCTGGGTAAA                                                                                     >  SRR4302542.7278153/1‑94 (MQ=60)
            TATTTGCACCGTATCGGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGTTGCTGGGTAAAG                                                                                    <  SRR4302542.6392044/94‑1 (MQ=60)
            TATTTGCACCGTATCGGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCTGCTGGGTAAAG                                                                                    <  SRR4302542.5824763/94‑1 (MQ=60)
              TTTGCACCGTATCGGACGTACCGCGCGCCCCGGGCGTAAAAGCCCCGCAATTTCGCTGGGTGAAGCCCATGACCAACTGGTTGTGGGTAAAGTA                                                                                  <  SRR4302542.4186947/94‑1 (MQ=44)
               TTGCACCGTATCGGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGTTGCTGGGTAAAGTAG                                                                                 <  SRR4302542.6811351/94‑1 (MQ=60)
               TTGCACCGTATCGGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGTTGCTGGGTAAAGTAG                                                                                 <  SRR4302542.1915364/94‑1 (MQ=60)
                 GCCCCGTATCGGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGTTGCTGGGTAAAGTAGGC                                                                               <  SRR4302542.3701565/94‑1 (MQ=60)
                 GCACCGTATCGGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGTTGCTGGGTAAAGTAGGC                                                                               <  SRR4302542.3657134/94‑1 (MQ=60)
                 GCACCGTATCGGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGTTGCTGGGTAAAGTAGGC                                                                               <  SRR4302542.289691/94‑1 (MQ=60)
                  CACCGTATCGGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGTTGCTGGGTAAAGTAGGCC                                                                              >  SRR4302542.3341690/1‑94 (MQ=60)
                  CACCGTATCGGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGTTGCTGGGTAAAGTAGGCC                                                                              >  SRR4302542.6127855/1‑94 (MQ=60)
                  CACCGTATCGGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGTTGCTGGGTAAAGTAGGCC                                                                              <  SRR4302542.5332862/94‑1 (MQ=60)
                  CACCGTATCGGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGTTGCTGGGTAAAGTAGGCC                                                                              <  SRR4302542.158126/94‑1 (MQ=60)
                  CACCGTATCGGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGTTGCTGGGTAAAGTAGGCC                                                                              >  SRR4302542.1949745/1‑94 (MQ=60)
                   ACCGTATCGGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGTTGCTGGGTAAAGTAGGCCG                                                                             >  SRR4302542.4971428/1‑94 (MQ=60)
                   ACCGTATCGGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGTTGCTGGGTAAAGTAGGCCG                                                                             >  SRR4302542.6934904/1‑94 (MQ=60)
                   ACCGTATCGGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCTGCTGGGTAAAGTAGGCCG                                                                             >  SRR4302542.5043589/1‑94 (MQ=60)
                    CCGTATCGGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGTTGCTGGGTAAAGTAGGCCGC                                                                            >  SRR4302542.259530/1‑94 (MQ=60)
                    CCGTATCGGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGTTGCTGGGTAAAGTAGGCCGC                                                                            >  SRR4302542.4979721/1‑94 (MQ=60)
                      GTATCGGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGTTGCTGGGTAAAGTAGGCCGCTA                                                                          >  SRR4302542.5170600/1‑94 (MQ=60)
                       TATCGGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGTTGCTGGGTAAAGTAGGCCGCTAT                                                                         <  SRR4302542.804682/94‑1 (MQ=60)
                       TATCGGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGTTGCTGGGTAAAGTAGGCCGCTAT                                                                         <  SRR4302542.7398875/94‑1 (MQ=60)
                       TATCGGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGTTGCTGGGTAAAGTAGGCCGCTAT                                                                         <  SRR4302542.2047350/94‑1 (MQ=60)
                       TATCGGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGTTGCTGGGTAAAGTAGGCCGCTAT                                                                         <  SRR4302542.7304833/94‑1 (MQ=60)
                       TATCGGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCTGCTGGGTAAAGTAGGCCGCTAT                                                                         <  SRR4302542.7527241/94‑1 (MQ=60)
                        ATCGGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGTTGCTGGGTAAAGTAGGCCGCTATA                                                                        <  SRR4302542.6718717/94‑1 (MQ=60)
                        ATCGGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGTTGCTGGGTAAAGTAGGCCGCTATA                                                                        >  SRR4302542.6057512/1‑94 (MQ=60)
                          CGGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGTTGCTGGGTAAAGTAGGCCGCTATATT                                                                      >  SRR4302542.4246549/1‑94 (MQ=60)
                          CGGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGTTGCTGGGTAAAGTAGGCCGCTATATT                                                                      >  SRR4302542.6103832/1‑94 (MQ=60)
                           GGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGTTGCTGGGTAAAGTAGGCCGCTATATTG                                                                     <  SRR4302542.6887159/94‑1 (MQ=60)
                           GGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGTTGCTGGGTAAAGTAGGCCGCTATATTG                                                                     <  SRR4302542.4392023/94‑1 (MQ=60)
                           GGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGTTGCTGGGTAAAGTAGGCCGCTATATTG                                                                     >  SRR4302542.5299344/1‑94 (MQ=60)
                           GGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGTTGCTGGGTAAAGTAGGCCGCTATATTG                                                                     <  SRR4302542.5622251/94‑1 (MQ=60)
                             ACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGTTGCTGGGTAAAGTAGGCCGCTATATTG‑AA                                                                  <  SRR4302542.6645615/94‑1 (MQ=60)
                              CGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGTTGCTGGGTAAAGTAGGCCGCTATATTG‑AAG                                                                 >  SRR4302542.2667143/1‑94 (MQ=60)
                               GTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGTTGCTGGGTAAAGTAGGCCGCTATATTG‑AAGA                                                                >  SRR4302542.5297929/1‑94 (MQ=60)
                               GTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGTTGCTGGGTAAAGTAGGCCGCTATATTG‑AAGA                                                                >  SRR4302542.5234873/1‑94 (MQ=60)
                               GTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGTTGCTGGGTAAAGTAGGCCGCTATATTG‑AAGA                                                                >  SRR4302542.6376004/1‑94 (MQ=60)
                                TACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCTGCTGGGTAAAGTAGGCCGCTATATTG‑AAGAG                                                               <  SRR4302542.2203845/94‑1 (MQ=60)
                                TACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGCAAGCCCATGACCATCTGCTGCTGGGTAAAGTAGGCCGCTATATTG‑AAGAG                                                               <  SRR4302542.7372364/94‑1 (MQ=60)
                                 ACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGTTGCTGGGTAAAGTAGGCCGCTATATTG‑AAGAGC                                                              >  SRR4302542.6706104/1‑94 (MQ=60)
                                   CGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGTTGCTGGGTAAAGTAGGCCGCTATATTG‑AAGAGCCA                                                            >  SRR4302542.1154214/1‑94 (MQ=60)
                                     CGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGTTGCTGGGTAAAGTAGGCCGCTATATTG‑AAGAGCCAAT                                                          <  SRR4302542.2484547/94‑1 (MQ=60)
                                     CGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGTTGCTGGGTAAAGTAGGCCGCTATATTG‑AAGAGCCAAT                                                          >  SRR4302542.2052476/1‑94 (MQ=60)
                                      GCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGTTGCTGGGTAAAGTAGGCCGCTATATTG‑AAGAGCCAATT                                                         <  SRR4302542.5842987/94‑1 (MQ=60)
                                      GCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGTTGCTGGGTAAAGTAGGCCGCTATATTG‑AAGAGCCAATT                                                         <  SRR4302542.7369758/94‑1 (MQ=60)
                                         CGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGTTGCTGGGTAAAGTAGGCCGCTATATTG‑AAGAGCCAATTAAA                                                      <  SRR4302542.7338989/94‑1 (MQ=60)
                                          GCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGTTGCTGGGTAAAGTAGGCCGCTATATTG‑AAGAGCCAATTAAAG                                                     <  SRR4302542.3417864/94‑1 (MQ=60)
                                           CCNNNNGNAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCANCTGTTGCTGGGTAAAGTAGGCCGCTATATTG‑AAGAGCCAATTAAAGC                                                    <  SRR4302542.6477603/94‑1 (MQ=60)
                                           CCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGTTGCTGGGTAAAGTAGGCCGCTATATTG‑AAGAGCCAATTAAAGC                                                    <  SRR4302542.1146163/94‑1 (MQ=60)
                                           CCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGTTGCTGGGTAAAGTAGGCCGCTATATTG‑AAGAGCCAATTAAAGC                                                    >  SRR4302542.7226955/1‑94 (MQ=60)
                                           CCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGTTGCTGGGTAAAGTAGGCCGCTATATTG‑AAGAGCCAATTAAAGC                                                    <  SRR4302542.5297379/94‑1 (MQ=60)
                                           CCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGTTGCTGGGTAAAGTAGGCCGCTATATTG‑AAGAGCCAATTAAAGC                                                    <  SRR4302542.5152970/94‑1 (MQ=60)
                                            CGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGTTGCTGGGTAAAGTAGGCCGCTATATTG‑AAGAGCCAATTAAAGCT                                                   >  SRR4302542.6397331/1‑94 (MQ=60)
                                            CGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGTTGCTGGGTAAAGTAGGCCGCTATATTG‑AAGAGCCAATTAAAGCT                                                   <  SRR4302542.1720984/94‑1 (MQ=60)
                                            CGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCTGCTGGGTAAAGTAGGCCGCTATATTG‑AAGAGCCAATTAAAGCT                                                   <  SRR4302542.913815/94‑1 (MQ=60)
                                             GGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCTGCTGGGTAAAGTAGGCCGCTATATTG‑AAGAGCCAATTAAAGCTC                                                  >  SRR4302542.7108227/1‑94 (MQ=60)
                                              GTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGTTGCTGGGTAAAGTAGGCCGCTATATTG‑AAGAGCCAATTAAAGCTCG                                                 <  SRR4302542.6299825/94‑1 (MQ=60)
                                              GTCGTAAAAGCACCGCAATTTCGCTGGGGGAAGCCCATGACCATCTGGTGCTGGGTAAAGCAGGCCGCCATATTG‑AAAAGCCAATTAAAGCTGG                                                 >  SRR4302542.3711982/1‑94 (MQ=60)
                                                CGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGTTGCTGGGTAAAGTAGGCCGCTATATTG‑AAGAGCCAATTAAAGCTCGCG                                               >  SRR4302542.762864/1‑94 (MQ=60)
                                                CGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGTTGCTGGGTAAAGTAGGCCGCTATATTG‑AAGAGCCAATTAAAGCTCGCG                                               <  SRR4302542.3106597/94‑1 (MQ=60)
                                                CGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGTTGCTGGGTAAAGTAGGCCGCTATATTG‑AAGAGCCAATTAAAGCTCGCG                                               >  SRR4302542.6955742/1‑94 (MQ=60)
                                                CGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGTTGCTGGGTAAAGTAGGCCGCTATATTG‑AAGAGCCAATTAAAGCTCGCG                                               >  SRR4302542.2468045/1‑94 (MQ=60)
                                                 GTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGTTGCTGGGTAAAGTAGGCCGCTATATTG‑AAGAGCCAATTAAAGCTCGCGT                                              >  SRR4302542.4927648/1‑94 (MQ=60)
                                                 GTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGTTGCTGGGTAAAGTAGGCCGCTATATTG‑AAGAGCCAATTAAAGCTCGCGT                                              >  SRR4302542.6635040/1‑94 (MQ=60)
                                                 GTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGTTGCTGGGTAAAGTAGGCCGCTATATTG‑AAGAGCCAATTAAAGCTCGCGT                                              >  SRR4302542.5952711/1‑94 (MQ=60)
                                                   AAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGTTGCTGGGTAAAGTAGGCCGCTATATTG‑AAGAGCCAATTAAAGCTCGCGTTA                                            >  SRR4302542.5197809/1‑94 (MQ=60)
                                                   AAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGTTGCTGGGTAAAGTAGGCCGCTATATTG‑AAGAGCCAATTAAAGCTCGCGTTA                                            <  SRR4302542.6799794/94‑1 (MQ=60)
                                                    AAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGTTGCTGGGTAAAGTAGGCCGCTATATTG‑AAGAGCCAATTAAAGCTCGCGTTAT                                           >  SRR4302542.4268216/1‑94 (MQ=60)
                                                     AGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCTGCTGGGTAAAGTAGGCCGCTATATTG‑AAGAGCCAATTAAAGCTCGCGTTATT                                          <  SRR4302542.25574/94‑1 (MQ=60)
                                                       GCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGTTGCTGGGTAAAGTAGGCCGCTATATTG‑AAGAGCCAATTAAAGCTCGCGTTATTGA                                        <  SRR4302542.4267820/94‑1 (MQ=60)
                                                       GCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCTGCTGGGTAAAGTAGGCCGCTATATTG‑AAGAGCCAATTAAAGCTCGCGTTATTGA                                        <  SRR4302542.3062112/94‑1 (MQ=60)
                                                        CACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGTTGCTGGGTAAAGTAGGCCGCTATATTG‑AAGAGCCAATTAAAGCTCGCGTTATTGAT                                       <  SRR4302542.5443754/94‑1 (MQ=60)
                                                        CACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGTTGCTGGGTAAAGTAGGCCGCTATATTG‑AAGAGCCAATTAAAGCTCGCGTTATTGAT                                       >  SRR4302542.1471983/1‑94 (MQ=60)
                                                         ACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGTTGCTGGGTAAAGTAGGCCGCTATATTG‑AAGAGCCAATTAAAGCTCGCGTTATTGATG                                      <  SRR4302542.1371581/94‑1 (MQ=60)
                                                         ACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGTTGCTGGGTAAAGTAGGCCGCTATATTG‑AAGAGCCAATTAAAGCTCGCGTTATTGATG                                      >  SRR4302542.5181520/1‑94 (MQ=60)
                                                            GCAATTTCGCTGGTGGAAGCCCATGACCATCTGTTGCTGGGTAAAGTAGGCCGCTATATTG‑AAGAGCCAATTAAAGCTCGCGTTATTGATGAGT                                   <  SRR4302542.1341199/94‑1 (MQ=60)
                                                             CAATTTCGCTGGTGGAAGCCCATGACCATCTGTTGCTGGGTAAAGTAGGCCGCTATATTG‑AAGAGCCAATTAAAGCTCGCGTTATTGATGAGTT                                  <  SRR4302542.2846942/94‑1 (MQ=60)
                                                              AATTTCGCTGGTGGAAGCCCATGACCATCTGTTGCTGGGTAAAGTAGGCCGCTATATTG‑AAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTA                                 >  SRR4302542.3516355/1‑94 (MQ=60)
                                                               ATTTCGCTGGTGGAAGCCCATGACCATCTGTTGCTGGGTAAAGTAGGCCGCTATATTG‑AAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTAC                                <  SRR4302542.3116411/94‑1 (MQ=60)
                                                               ATTTCGCTGGTGGAAGCCCATGACCATCTGTTGCTGGGTAAAGTAGGCCGCTATATTG‑AAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTAC                                <  SRR4302542.1709029/94‑1 (MQ=60)
                                                                TTTCGCTGGTGGAAGCCCATGACCATCTGTTGCTGGGTAAAGTAGGCCGCTATATTG‑AAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTACG                               <  SRR4302542.864104/94‑1 (MQ=60)
                                                                TTTCGCTGGTGGAAGCCCATGACCATCTGTTGCTGGGTAAAGTAGGCCGCTATATTG‑AAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTACG                               <  SRR4302542.6063397/94‑1 (MQ=60)
                                                                  TCGCTGGTGGAAGCCCATGACCATCTGTTGCTGGGTAAAGTAGGCCGCTATATTG‑AAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTACGCC                             <  SRR4302542.1079608/94‑1 (MQ=60)
                                                                    GCTGGTGGAAGCCCATGACCATCTGTTGCTGGGTAAAGTAGGCCGCTATATTG‑AAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTACGCCCG                           <  SRR4302542.1023018/94‑1 (MQ=60)
                                                                      TGGTGGAAGCCCATGACCATCTGCTGCTGGGTAAAGTAGGCCGCTATATTG‑AAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTACGCCCGAA                         >  SRR4302542.6717914/1‑94 (MQ=60)
                                                                        GTGGAAGCCCATGACCATCTGTTGCTGGGTAAAGTAGGCCGCTATATTG‑AAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTACGCCCGAAAA                       >  SRR4302542.218748/1‑94 (MQ=60)
                                                                        GTGGAAGCCCATGACCATCTGTTGCTGGGTAAAGTAGGCCGCTATATTG‑AAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTACGCCCGAAAA                       >  SRR4302542.2292063/1‑94 (MQ=60)
                                                                          GGAAGCCCATGACCATCTGTTGCTGGGTAAAGTAGGCCGCTATATTG‑AAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTACGCCCGAAAACG                     <  SRR4302542.386335/94‑1 (MQ=60)
                                                                              GCCCATGACCATCTGTTGCTGGGTAAAGTAGGCCGCTATATTG‑AAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTACGCCCGAAAACGCGTG                 <  SRR4302542.3874402/94‑1 (MQ=60)
                                                                              GCCCATGACCATCTGTTGCTGGGTAAAGTAGGCCGCTATATTG‑AAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTACGCCCGAAAACGCGTG                 <  SRR4302542.6885241/94‑1 (MQ=60)
                                                                               CCCATGACCATCTGTTGCTGGGTAAAGTAGGCCGCTATATTG‑AAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTACGCCCGAAAACGCGTGC                >  SRR4302542.2233227/1‑94 (MQ=60)
                                                                               CCCATGACCATCTGTTGCTGGGTAAAGTAGGCCGCTATATTG‑AAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTACGCCCGAAAACGCGTGC                >  SRR4302542.579174/1‑94 (MQ=60)
                                                                               CCCATGACCATCTGTTGCTGGGTAAAGTAGGCCGCTATATTG‑AAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTACGCCCGAAAACGCGTGC                <  SRR4302542.6111009/94‑1 (MQ=60)
                                                                                 CATGACCATCTGTTGCTGGGTAAAGTAGGCCGCTATATTG‑AAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTACGCCCGAAAACGCGTGCGC              >  SRR4302542.1198041/1‑94 (MQ=60)
                                                                                 CATGACCATCTGTTGCTGGGTAAAGTAGGCCGCTATATTG‑AAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTACGCCCGAAAACGCGTGCGC              >  SRR4302542.3040582/1‑94 (MQ=60)
                                                                                  ATGACCATCTGTTGCTGGGTAAAGTAGGCCGCTATATTG‑AAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTACGCCCGAAAACGCGTGCGCC             >  SRR4302542.4197041/1‑94 (MQ=60)
                                                                                   TGACCATCTGTTGCTGGGTAAAGTAGGCCGCTATATTG‑AAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTACGCCCGAAAACGCGTGCGCCA            <  SRR4302542.3337997/94‑1 (MQ=60)
                                                                                     ACCATCTGTTGCTGGGTAAAGTAGGCCGCTATATTG‑AAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTACGCCCGAAAACGCGTGCGCCAAG          >  SRR4302542.5856069/1‑94 (MQ=60)
                                                                                     ACCATCTGTTGCTGGGTAAAGTAGGCCGCTATATTG‑AAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTACGCCCGAAAACGCGTGCGCCAAG          >  SRR4302542.1911559/1‑94 (MQ=60)
                                                                                     ACCATCTGTTGCTGGGTAAAGTAGGCCGCTATATTG‑AAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTACGCCCGAAAACGCGTGCGCCAAG          >  SRR4302542.1421166/1‑94 (MQ=60)
                                                                                      CCATCTGTTGCTGGGTAAAGTAGGCCGCTATATTG‑AAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTACGCCCGAAAACGCGTGCGCCAAGC         <  SRR4302542.4487433/94‑1 (MQ=60)
                                                                                       CATCTGTTGCTGGGTAAAGTAGGCCGCTATATTG‑AAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTACGCCCGAAAACGCGTGCGCCAAGCG        <  SRR4302542.2215169/94‑1 (MQ=60)
                                                                                        ATCTGTTGCTGGGTAAAGTAGGCCGCTATATTG‑AAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTACGCCCGAAAACGCGTGCGCCAAGCGA       <  SRR4302542.4586232/94‑1 (MQ=60)
                                                                                        ATCTGTTGCTGGGTAAAGTAGGCCGCTATAATGAAAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTACGCCCGAAAACGCGTGCGCCAAGCG        <  SRR4302542.3756738/94‑1 (MQ=60)
                                                                                         TCTGTTGCTGGGTAAAGTAGGCCGCTATATTG‑AAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTACGCCCGAAAACGCGTGCGCCAAGCGAA      >  SRR4302542.4282003/1‑94 (MQ=60)
                                                                                         TCTGTTGCTGGGTAAAGTAGGCCGCTATATTG‑AAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTACGCCCGAAAACGCGTGCGCCAAGCGAA      <  SRR4302542.6214811/94‑1 (MQ=60)
                                                                                           TGTTGCTGGGTAAAGTAGGCCGCTATATTG‑AAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTACGCCCGAAAACGCGTGCGCCAAGCGAAAA    <  SRR4302542.1741591/94‑1 (MQ=60)
                                                                                            GTTGCTGGGTAAAGTAGGCCGCTATATTG‑AAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTACGCCCGAAAACGCGTGCGCCAAGCGAAAAG   <  SRR4302542.5124931/94‑1 (MQ=60)
                                                                                             CTGCTGGGTAAAGTAGGCCGCTATATTG‑AAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTACGCCCGAAAACGCGTGCGCCAAGCGAAAAGC  >  SRR4302542.4837451/1‑94 (MQ=60)
                                                                                             |                                                                                              
AGTGGCGATACTTATTTGCACCGTATCGGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCTGCTGGGTAAAGTAGGCCGCTATATTG‑AAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTACGCCCGAAAACGCGTGCGCCAAGCGAAAAGC  >  NC_000913/2711897‑2712083

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑