Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1061 294.1 15065038 98.6% 14854127 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,723,253 C→T G172R (GGA→AGA)  kgtP ← alpha‑ketoglutarate permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,723,2530CT86.5% 791.8 / 76.0 273G172R (GGA→AGA) kgtPalpha‑ketoglutarate permease
Reads supporting (aligned to +/- strand):  ref base C (20/17);  new base T (119/117);  total (139/134)
Fisher's exact test for biased strand distribution p-value = 7.26e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.11e-01

Only 100 of 287 total aligned reads displayed.

CGCGAAAGGAATACGCCATCCCCACTCTCTGAGTGCAGCGTCTTCCATGGTGTGTTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTCCGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGCCATATT  >  NC_000913/2723161‑2723345
                                                                                            |                                                                                            
  cgAAAGGAATACGCCATCCCCACTCTCTGAGTGCAGCGTCTTCCATGGTGTGTTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTctgc                                                                                            <  2:530835/93‑1 (MQ=255)
  cgAAAGGAATACGCCATCCCCACTCTCTGAGTGCAGCGTCTTCCATGGTGTGTTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTctgc                                                                                            >  2:5512704/1‑93 (MQ=255)
   gAAAGGAATACGCCATCCCCACTCTCTGAGTGCAGCGTCTTCCATGGTGTGTTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTccgcc                                                                                           >  2:5708192/1‑93 (MQ=255)
    aaaGGAATACGCCATCCCCACTCTCTGAGTGCAGCGTCTTCCATGGTGTGTTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTctgccg                                                                                          >  2:3312620/1‑93 (MQ=255)
    aaaGGAATACGCCATCCCCACTCTCTGAGTGCAGCGTCTTCCATGGTGTGTTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTctgccg                                                                                          >  2:5334369/1‑93 (MQ=255)
     aaGGAATACGCCATCCCCACTCTCTGAGTGCAGCGTCTTCCATGGTGTGTTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTCTGCCGa                                                                                         <  1:6777012/93‑1 (MQ=255)
      aGGAATACGCCATCCCCACTCTCTGAGTGCAGCGTCTTCCATGGTGTGTTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTCTGCCGAt                                                                                        <  1:6622711/93‑1 (MQ=255)
         aaTACGCCATCCCCACTCTCTGAGTGCAGCGTCTTCCATGGTGTGTTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTCTGCCGATCaa                                                                                     >  1:2121121/1‑93 (MQ=255)
           tACGCCATCCCCACTCTCTGAGTGCAGCGTCTTCCATGGTGTGTTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTCTGCCGATCAACg                                                                                   >  1:2899149/1‑93 (MQ=255)
            aCGCCATCCCCACTCTCTGAGTGCAGCGTCTTCCATGGTGTGTTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTCTGCCGATCAACGt                                                                                  <  1:6586121/93‑1 (MQ=255)
            aCGCCATCCCCACTCTCTGAGTGCAGCGTCTTCCATGGTGTGTTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTCTGCCGATCAACGt                                                                                  <  1:2374527/93‑1 (MQ=255)
             cGCCATCCCCACTCTCTGAGTGCAGCGTCTTCCATGGTGTGTTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTCTGCCGATCAACGTc                                                                                 <  2:1507415/93‑1 (MQ=255)
             cGCCATCCCCACTCTCTGAGTGCAGCGTCTTCCATGGTGTGTTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTCTGCCGATCAACGTc                                                                                 >  2:2957468/1‑93 (MQ=255)
               ccATCCCCACTCTCTGAGTGCAGCGTCTTCCATGGTGTGTTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTCTGCCGATCAACGTcac                                                                               >  2:4356346/1‑93 (MQ=255)
                ccatcccACTCTCTGAGTGCAGCGTCTTCCATGGTGTGTTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTCTGCCGATCAACGTcaca                                                                              <  2:6856661/89‑1 (MQ=255)
                 aTCCCCACTCTCTGAGTGCAGCGTCTTCCATGGTGTGTTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTCTGCCGATCAACGTCACAt                                                                             >  1:7221939/1‑93 (MQ=255)
                  tCCCCACTCTCTGAGTGCAGCGTCTTCCATGGTGTGTTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTCTGCCGATCAACGTCACATa                                                                            >  1:3955201/1‑93 (MQ=255)
                    cccACTCTCTGAGTGCAGCGTCTTCCATGGTGTGTTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTCTGCCGATCAACGTCACATACt                                                                          <  2:3901743/93‑1 (MQ=255)
                     ccACTCTCTGAGTGCAGCGTCTTCCATGGTGTGTTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTCTGCCGATCAACGTCACATACTg                                                                         >  2:6011334/1‑93 (MQ=255)
                      cACTCTCTGAGTGCAGCGTCTTCCATGGTGTGTTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTCTGCCGATCAACGTCACATACTGa                                                                        >  1:827696/1‑93 (MQ=255)
                      cACTCTCTGAGTGCAGCGTCTTCCATGGTGTGTTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTCTGCCGATCAACGTCACATACTGa                                                                        <  1:640889/93‑1 (MQ=255)
                      cACTCTCTGAGTGCAGCGTCTTCCATGGTGTGTTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTCTGCCGATCAACGTCACATACTGa                                                                        >  1:1306210/1‑93 (MQ=255)
                       aCTCTCTGAGTGCAGCGTCTTCCATGGTGTGTTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTCTGCCGATCAACGTCACATACTGaa                                                                       >  2:1476276/1‑93 (MQ=255)
                       aCTCTCTGAGTGCAGCGTCTTCCATGGTGTGTTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTCTGCCGATCAACGTCACATACTGaa                                                                       >  2:5004701/1‑93 (MQ=255)
                         tctctGTGTGCAGCGTCTTCCATGGTGTGTTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTCTGCCGATCAACGTCACATACTGAAat                                                                     >  2:4906259/1‑93 (MQ=255)
                           tctGAGTGCAGCGTCTTCCATGGTGTGTTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTCTGCCGATCAACGTCACATACTGAAatga                                                                   >  2:1330416/1‑93 (MQ=255)
                           tctGAGTGCAGCGTCTTCCATGGTGTGTTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTCTGCCGATCAACGTCACATACTGAAatga                                                                   >  1:1049239/1‑93 (MQ=255)
                             tGAGTGCAGCGTCTTCCATGGTGTGTTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTCTGCCGATCAACGTCACATACTGAAatgatg                                                                 >  1:4321352/1‑93 (MQ=255)
                             tGAGTGCAGCGTCTTCCATGGTGTGTTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTCTGCCGATCAACGTCACATACTGAAatgatg                                                                 <  1:54941/93‑1 (MQ=255)
                             tGAGTGCAGCGTCTTCCATGGTGTGTTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTCTGCCGATCAACGTCACATACTGAAatgatg                                                                 <  1:1127907/93‑1 (MQ=255)
                              gAGTGCAGCGTCTTCCATGGTGTGTTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTCTGCCGATCAACGTCACATACTGAAATGATGc                                                                <  2:1559831/93‑1 (MQ=255)
                              gAGTGCAGCGTCTTCCATGGTGTGTTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTCTGCCGATCAACGTCACATACTGAAATGATGc                                                                >  1:7123855/1‑93 (MQ=255)
                               aGTGCAGCGTCTTCCATGGTGTGTTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTCCGCCGATCAACGTCACATACTGAAATGATGCg                                                               >  2:5984304/1‑93 (MQ=255)
                                   cAGCGTCTTCCATGGTGTGTTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTCCGCCGATCAACGTCACATACTGAAATGATGCGTaaa                                                           <  1:4458615/93‑1 (MQ=255)
                                    aGCGTCTTCCATGGTGTGTTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTCCGCCGATCAACGTCACATACTGAAATGATGCGTaaaa                                                          >  2:903275/1‑93 (MQ=255)
                                     gCGTCTTCCATGGTGTGTTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTCTGCCGATCAACGTCACATACTGAAATGATGCGTaaaaa                                                         <  2:3669261/93‑1 (MQ=255)
                                      cGTCTTCCATGGTGTGTTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTCTGCCGATCAACGTCACATACTGAAATGATGCGTAAAAAc                                                        >  1:2585922/1‑93 (MQ=255)
                                       gTCTTCCATGGTGTGTTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTCTGCCGATCAACGTCACATACTGAAATGATGCGTAAAAAcc                                                       >  2:6380049/1‑93 (MQ=255)
                                        tCTTCCATGGTGTGTTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTCTGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCt                                                      >  2:6982649/1‑93 (MQ=255)
                                         cTTCCATGGTGTGTTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTCTGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCtt                                                     <  2:2658758/93‑1 (MQ=255)
                                         cTTCCATGGTGTGTTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTCTGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCtt                                                     <  1:5777565/93‑1 (MQ=255)
                                         cTTCCATGGTGTGTTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTCTGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCtt                                                     >  2:5051307/1‑93 (MQ=255)
                                          ttCCATGGTGTGTTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTCTGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCttt                                                    <  1:566845/93‑1 (MQ=255)
                                          ttCCATGGTGTGTTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTCTGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCttt                                                    >  2:3150053/1‑93 (MQ=255)
                                           tCCATGGTGTGTTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTCTGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTg                                                   <  2:1871584/93‑1 (MQ=255)
                                            ccATGGTGTGTTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTCTGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTgc                                                  <  1:6437035/93‑1 (MQ=255)
                                              aTGGTGTGTTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTCTGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTgcgc                                                <  1:5963906/93‑1 (MQ=255)
                                              aTGGTGTGTTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTCTGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTgcgc                                                >  1:922139/1‑93 (MQ=255)
                                              aTGGTGTGTTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTCCGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTgcgc                                                >  2:754493/1‑93 (MQ=255)
                                                ggTGTGTTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTCTGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGccc                                              >  1:4199706/1‑93 (MQ=255)
                                                ggTGTGTTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTCTGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGccc                                              <  1:2149107/93‑1 (MQ=255)
                                                 gtgtgtTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTCTGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCt                                             <  2:4299371/93‑1 (MQ=255)
                                                 gtgtgtTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTCTGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCt                                             >  2:3077398/1‑93 (MQ=255)
                                                 gtgtgtTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTCTGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCt                                             <  2:6818055/93‑1 (MQ=255)
                                                 gtgtgtTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTCCGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCt                                             <  2:2752110/93‑1 (MQ=255)
                                                  tgtgtTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTCTGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCtt                                            >  1:5883562/1‑93 (MQ=255)
                                                   gtgtTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTCTGTCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTc                                           <  1:3724044/93‑1 (MQ=255)
                                                     gttgtAAAACCACGACAACCAGTAGGGCTAGCAGTTGTCTGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCaa                                         <  2:7203194/93‑1 (MQ=255)
                                                     gttgtAAAACCACGACAACCAGTAGGGCTAGCAGTTGTCCGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCaa                                         <  1:4945080/93‑1 (MQ=255)
                                                      ttgtAAAACCACGACAACCAGTAGGGCTAGCAGTTGTCTGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAAc                                        >  1:783984/1‑93 (MQ=255)
                                                        gtAAAACCACGACAACCAGTAGGGCTAGCAGTTGTCTGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACgg                                      >  2:5191671/1‑93 (MQ=255)
                                                        gtAAAACCACGACAACCAGTAGGGCTAGCAGTTGTCTGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACgg                                      <  2:4574312/93‑1 (MQ=255)
                                                         tAAAACCACGACAACCAGTAGGGCTAGCAGTTGTCTGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGc                                     >  2:4488717/1‑93 (MQ=255)
                                                         tAAAACCACGACAACCAGTAGGGCTAGCAGTTGTCTGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGc                                     <  1:6221055/93‑1 (MQ=255)
                                                           aaaCCACGACAACCAGTAGGGCTAGCAGTTGTCTGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCaa                                   <  2:2093793/93‑1 (MQ=255)
                                                           aaaCCACGACAACCAGTAGGGCTAGCAGTTGTCTGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCaa                                   <  1:146983/93‑1 (MQ=255)
                                                           aaaCCACGACAACCAGTAGGGCTAGCAGTTGTCTGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCaa                                   <  2:4707442/93‑1 (MQ=255)
                                                            aaCCACGACAACCAGTAGGGCTAGCAGTTGTCTGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAAc                                  >  2:5966737/1‑93 (MQ=255)
                                                               cACGACAACCAGTAGGGCTAGCAGTTGTCTGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTc                               >  1:1670407/1‑93 (MQ=255)
                                                                aCGACAACCAGTAGGGCTAGCAGTTGTCTGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCa                              >  1:3828904/1‑93 (MQ=255)
                                                                   aCAACCAGTAGGGCTAGCAGTTGTCTGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTc                           >  1:2770146/1‑93 (MQ=255)
                                                                   aCAACCAGTAGGGCTAGCAGTTGTCCGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTc                           >  1:2800725/1‑93 (MQ=255)
                                                                    cAACCAGTAGGGCTAGCAGTTGTCTGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCa                          <  1:7442448/93‑1 (MQ=255)
                                                                    cAACCAGTAGGGCTAGCAGTTGTCCGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCa                          <  2:6138902/93‑1 (MQ=255)
                                                                     aaCCAGTAGGGCTAGCAGTTGTCTGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCat                         >  1:1907553/1‑93 (MQ=255)
                                                                     aaCCAGTAGGGCTAGCAGTTGTCTGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCat                         >  1:4535552/1‑93 (MQ=255)
                                                                     aaCCAGTAGGGCTAGCAGTTGTCTGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCat                         >  1:7014762/1‑93 (MQ=255)
                                                                      aCCAGTAGGGCTAGCAGTTGTCTGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCata                        >  1:5214357/1‑93 (MQ=255)
                                                                       ccAGTAGGGCTAGCAGTTGTCTGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCatat                       <  1:117811/93‑1 (MQ=255)
                                                                       ccAGTAGGGCTAGCAGTTGTCTGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCatat                       >  1:6008253/1‑93 (MQ=255)
                                                                        cAGTAGGGCTAGCAGTTGTCTGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCAtata                      <  1:2139556/93‑1 (MQ=255)
                                                                        cAGTAGGGCTAGCAGTTGTCTGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCAtata                      <  1:7313538/93‑1 (MQ=255)
                                                                        cAGTAGGGCTAGCAGTTGTCTGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCAtata                      <  2:2372089/93‑1 (MQ=255)
                                                                        cAGTAGGGCTAGCAGTTGTCTGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCAtata                      >  2:6677019/1‑93 (MQ=255)
                                                                         aGTAGGGCTAGCAGTTGTCCGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAg                     >  1:6253982/1‑93 (MQ=255)
                                                                          gTAGGGCTAGCAGTTGTCTGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAgg                    >  2:2177183/1‑93 (MQ=255)
                                                                           tAGGGCTAGCAGTTGTCTGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGt                   <  2:5817885/93‑1 (MQ=255)
                                                                           tAGGGCTAGCAGTTGTCTGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGt                   >  1:6823987/1‑93 (MQ=255)
                                                                           tAGGGCTAGCAGTTGTCTGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGt                   <  1:4850574/93‑1 (MQ=255)
                                                                            aGGGCTAGCAGTTGTCTGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTg                  >  2:4645838/1‑93 (MQ=255)
                                                                            aGGGCTAGCAGTTGTCTGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTg                  <  2:1262767/93‑1 (MQ=255)
                                                                              ggCTAGCAGTTGTCTGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGgc                >  1:5058859/1‑93 (MQ=255)
                                                                                cTAGCAGTTGTCTGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGgcgc              >  1:3159124/1‑93 (MQ=255)
                                                                                 tAGCAGTTGTCTGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCt             >  1:3358037/1‑93 (MQ=255)
                                                                                  aGCAGTTGTCTGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTg            >  2:6498805/1‑93 (MQ=255)
                                                                                  aGCAGTTGTCTGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTg            <  2:7411022/93‑1 (MQ=255)
                                                                                    cAGTTGTCTGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGt          <  2:2475730/93‑1 (MQ=255)
                                                                                      gTTGTCTGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGc        >  2:7240534/1‑93 (MQ=255)
                                                                                      gTTGTCTGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGc        <  1:3185173/93‑1 (MQ=255)
                                                                                        tGTCTGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGCCa      <  2:709866/93‑1 (MQ=255)
                                                                                            |                                                                                            
CGCGAAAGGAATACGCCATCCCCACTCTCTGAGTGCAGCGTCTTCCATGGTGTGTTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTCCGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGCCATATT  >  NC_000913/2723161‑2723345

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ACGCGAAAGGAATACGCCATCCCCACTCTCTGAGTGCAGCGTCTTCCATGGTGTGTTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTCCGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGCCATATTC  >  NC_000913/2723160‑2723346
                                                                                             |                                                                                             
ACGCGAAAGGAATACGCCATCCCCACTCTCTGAGTGCAGCGTCTTCCATGGTGTGTTGTAAAACCACTACAACCAGTAGGGCTAGCAGTTGTCT                                                                                               <  SRR4302542.4685399/94‑1 (MQ=60)
 CGCGAAAGGAATACGCCATCCCCACTCTCTGAGTGCAGCGTCTTCCATGGTGTGTTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTCTG                                                                                              >  SRR4302542.3842402/1‑94 (MQ=60)
 CGCGAAAGGAATACGCCATCCCCACTCTCTGAGTGCAGCGTCTTCCATGGTGTGTTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTCCA                                                                                              <  SRR4302542.6849980/94‑1 (MQ=60)
   CGAAAGGAATACGCCATCCCCACTCTCTGAGTGCAGNNNCTNCCATGGTGTGTTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTCNNCC                                                                                            >  SRR4302542.5456852/1‑94 (MQ=60)
   CGAAAGGAATACGCCATCCCCACTCTCTGAGTGCAGCGTCTTCCATGGTGTGTTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTCTGCC                                                                                            >  SRR4302542.6295381/1‑94 (MQ=60)
    GAAAGGAATACGCCATCCCCACTCTCTGAGTGCAGCGTCTTCCATGGTGTGTTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTCTGCCG                                                                                           <  SRR4302542.4696824/94‑1 (MQ=60)
    GAAAGGAATACGCCATCCCCACTCTCTGAGTGCAGCGTCTTCCATGGTGTGTTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTCTGCCG                                                                                           >  SRR4302542.6887776/1‑94 (MQ=60)
      AAGGAATACGCCATCCCCACTCTCTGAGTGCAGCGTCTTCCATGGTGTGTTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTCTGCCGAT                                                                                         <  SRR4302542.6843680/94‑1 (MQ=60)
       AGGAATACGCCATCCCCACTCTCTGAGTGCAGCGTCTTCCATGGTGTGTTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTCTGCCGATC                                                                                        <  SRR4302542.290396/94‑1 (MQ=60)
       AGGAATACGCCATCCCCACTCTCTGAGTGCAGCGTCTTCCATGGTGTGTTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTCTGCCGATC                                                                                        <  SRR4302542.6688007/94‑1 (MQ=60)
         GAATACGCCATCCCCACTCTCTGAGTGCAGCGTCTTCCATGGTGTGTTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTCTGCCGATCAA                                                                                      >  SRR4302542.2139425/1‑94 (MQ=60)
          AATACGCCATCCCCACTCTCTGAGTGCAGCGTCTTCCATGGTGTGTTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTCCGCCGATCAAC                                                                                     >  SRR4302542.2806390/1‑94 (MQ=60)
           ATACGCCATCCCCACTCTCTGAGTGCAGCGTCTTCCATGGTGTGTTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTCTGCCGATCAACG                                                                                    <  SRR4302542.5920432/94‑1 (MQ=60)
           ATACGCCATCCCCACTCTCTGAGTGCAGCGTCTTCCATGGTGTGTTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTCTGCCGATCAACG                                                                                    >  SRR4302542.2926973/1‑94 (MQ=60)
             ACGCCATCCCCACTCTCTGAGTGCAGCGTCTTCCATGGTGTGTTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTCTGCCGATCAACGTC                                                                                  <  SRR4302542.2395648/94‑1 (MQ=60)
             ACGCCATCCCCACTCTCTGAGTGCAGCGTCTTCCATGGTGTGTTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTCTGCCGATCAACGTC                                                                                  <  SRR4302542.6651010/94‑1 (MQ=60)
              CGCCATCCCCACTCTCTGAGTGCAGCGTCTTCCATGGTGTGTTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTCTGCCGATCAACGTCA                                                                                 >  SRR4302542.1158086/1‑94 (MQ=60)
                CCATGCCCACTCTCTGAGTGCAGCGTCTTCCATGGTGTGTTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTCTGCCGATCAACGTCACA                                                                               >  SRR4302542.7037239/1‑94 (MQ=60)
                CCATCCCCACTCTCTGAGTGCAGCGTCTTCCATGGTGTGTTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTCTGCCGATCAACGTCACA                                                                               <  SRR4302542.5559600/94‑1 (MQ=60)
                CCATCCCCACTCTCTGAGTGCAGCGTCTTCCATGGTGTGTTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTCTGCCGATCAACGTCACA                                                                               >  SRR4302542.7192389/1‑94 (MQ=60)
                 CATCCCCACTCTCTGAGTGCAGCGTCTTCCATGGTGTGTTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTCTGCCGATCAACGTCACAT                                                                              <  SRR4302542.6633729/94‑1 (MQ=60)
                 CATCCCCACTCTCTGAGTGCAGCGTCTTCCATGGTGTGTTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTCTGCCGATCAACGTCACAT                                                                              >  SRR4302542.6047301/1‑94 (MQ=60)
                 CATCCCCACTCTCTGAGTGCAGCGTCTTCCATGGTGTGTTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTCTGCCGATCAACGTCACAT                                                                              >  SRR4302542.7292781/1‑94 (MQ=60)
                 CATCCCCACTCTCTGAGTGCAGCGTCTTCCATGGTGTGTTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTCCGCCGATCAACGTCACAT                                                                              >  SRR4302542.4898916/1‑94 (MQ=60)
                  ATCCCCACTCTCTGAGTGCAGCGTCTTCCATGGTGTGTTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTCTGCCGATCAACGTCACATA                                                                             >  SRR4302542.4948784/1‑94 (MQ=60)
                  ATCCCCACTCTCTGAGTGCAGCGTCTTCCATGGTGTGTTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTCTGCCGATCAACGTCACATA                                                                             <  SRR4302542.193958/94‑1 (MQ=60)
                  ATCCCCACTCTCTGAGTGCAGCGTCTTCCATGGTGTGTTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTCTGCCGATCAACGTCACATA                                                                             >  SRR4302542.2896640/1‑94 (MQ=60)
                  ATCCCCACTCTCTGAGTGCAGCGTCTTCCATGGTGTGTTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTCTGCCGATCAACGTCACATA                                                                             >  SRR4302542.7597102/1‑94 (MQ=60)
                  ATCCCCACTCTCTGAGTGCAGCGTCTTCCATGGTGTGTTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTCTGCCGATCAACGTCACATA                                                                             >  SRR4302542.3994247/1‑94 (MQ=60)
                   TCCCCACTCTCTGAGTGCAGCGTCTTCCATGGTGTGTTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTCTGCCGATCAACGTCACATAC                                                                            <  SRR4302542.6725609/94‑1 (MQ=60)
                   TCCCCACTCTCTGAGTGCAGCGTCTTCCATGGTGTGTTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTCCGCCGATCAACGTCACATAC                                                                            <  SRR4302542.7208892/94‑1 (MQ=60)
                    CCCCACTCTCTGAGTGCAGCGTCTTCCATGGTGTGTTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTCTGCCGATCAACGTCACATACT                                                                           <  SRR4302542.6011045/94‑1 (MQ=60)
                    CCCCACTCTCTGAGTGCAGCGTCTTCCATGGTGTGTTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTCTGCCGATCAACGTCACATACT                                                                           <  SRR4302542.1799632/94‑1 (MQ=60)
                    CCCCACTCTCTGAGTGCAGCGTCTTCCATGGTGTGTTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTCTGCCGATCAACGTCACATACT                                                                           >  SRR4302542.5352809/1‑94 (MQ=60)
                    CCCCACTCTCTGAGTGCAGCGTCTTCCATGGTGTGTTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTCTGCCGATCAACGTCACATACT                                                                           <  SRR4302542.2163860/94‑1 (MQ=60)
                    CCCCACTCTCTGAGTGCAGCGTCTTCCATGGTGTGTTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTCTGCCGATCAACGTCACATACT                                                                           >  SRR4302542.666224/1‑94 (MQ=60)
                    CCCCACTCTCTGAGTGCAGCGTCTTCCATGGTGTGTTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTCCGCCGATCAACGTCACATACT                                                                           <  SRR4302542.858972/94‑1 (MQ=60)
                    CCCCACTCTCTGAGTGCAGCGTCTTCCATGGTGTGTTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTCCGCCGATCAACGTCACATACT                                                                           >  SRR4302542.5714101/1‑94 (MQ=60)
                      CCACTCTCTGAGTGCAGCGTCTTCCATGGTGTGTTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTCTGCCGATCAACGTCACATACTGA                                                                         >  SRR4302542.834849/1‑94 (MQ=60)
                      CCACTCTCTGAGTGCAGCGTCTTCCATGGTGTGTTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTCTGCCGATCAACGTCACATACTGA                                                                         >  SRR4302542.1317470/1‑94 (MQ=60)
                      CCACTCTCTGAGTGCAGCGTCTTCCATGGTGTGTTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTCTGCCGATCAACGTCACATACTGA                                                                         >  SRR4302542.6006071/1‑94 (MQ=60)
                      CCACTCTCTGAGTGCAGCGTCTTCCATGGTGTGTTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTCTGCCGATCAACGTCACATACTGA                                                                         >  SRR4302542.1544133/1‑94 (MQ=60)
                       CACTCTCTGAGTGCAGCGTCTTCCATGGTGTGTTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTCTGCCGATCAACGTCACATACTGAA                                                                        <  SRR4302542.646403/94‑1 (MQ=60)
                        ACTCTCTGAGTGCAGCGTCTTCCATGGTGTGTTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTCTGCCGATCAACGTCACATACTGAAA                                                                       >  SRR4302542.2627828/1‑94 (MQ=60)
                          TCTCTGAGTGCAGCGTCTTCCATGGTGTGTTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTCTGCCGATCAACGTCACATACTGAAATG                                                                     >  SRR4302542.3326346/1‑94 (MQ=60)
                          TCTCTGAGTGCAGCGTCTTCCATGGTGTGTTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTCTGCCGATCAACGTCACATACTGAAATG                                                                     <  SRR4302542.5195834/94‑1 (MQ=60)
                           CTCTGAGTGCAGCGTCTTCCATGGTGTGTTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTCTGCCGATCAACGTCGCATACTGAAATGA                                                                    <  SRR4302542.1469940/94‑1 (MQ=60)
                           CTCTGAGTGCAGCGTCTTCCATGGTGTGTTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTCTGCCGATCAACGTCACATACTGAAATGA                                                                    >  SRR4302542.6393013/1‑94 (MQ=60)
                           CTCTGAGTGCAGCGTCTTCCATGGTGTGTTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTCTGCCGATCAACGTCACATACTGAAATGA                                                                    >  SRR4302542.1058266/1‑94 (MQ=60)
                            TCTGAGTGCAGCGTCTTCCATGGTGTGTTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTCTGCCGATCAACGTCACATACTGAAATGAT                                                                   >  SRR4302542.1143828/1‑94 (MQ=60)
                             CTGAGTGCAGCGTCTTCCATGGTGTGTTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTCTGCCGATCAACGTCACATACTGAAATGATG                                                                  >  SRR4302542.4363836/1‑94 (MQ=60)
                             CTGAGTGCAGCGTCTTCCATGGTGTGTTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTCTGCCGATCAACGTCACATACTGAAATGATG                                                                  >  SRR4302542.3803568/1‑94 (MQ=60)
                             CTGAGTGCAGCGTCTTCCATGGTGTGTTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTCCGCCGATCAACGTCACATACTGAAATGATG                                                                  >  SRR4302542.6670430/1‑94 (MQ=60)
                             CTGAGTGCAGCGTCTTCCATGGTGTGTTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTCCGCCGATCAACGTCACATACTGAAATGATG                                                                  <  SRR4302542.6498867/94‑1 (MQ=60)
                              TGAGTGCAGCGTCTTCCATGGTGTGTTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTCTGCCGATCAACGTCACATACTGAAATGATGC                                                                 >  SRR4302542.7193777/1‑94 (MQ=60)
                              TGAGTGCAGCGTCTTCCATGGTGTGTTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTCTGCCGATCAACGTCACATACTGAAATGATGC                                                                 <  SRR4302542.55389/94‑1 (MQ=60)
                              TGAGTGCAGCGTCTTCCATGGTGTGTTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTCTGCCGATCAACGTCACATACTGAAATGATGC                                                                 <  SRR4302542.1137533/94‑1 (MQ=60)
                               GAGTGCAGCGTCTTCCATGGTGTGTTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTCTGCCGATCAACGTCACATACTGAAATGATGCG                                                                <  SRR4302542.5079454/94‑1 (MQ=60)
                               GAGTGCAGCGTCTTCCATGGTGTGTTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTCTGCCGATCAACGTCACATACTGAAATGATGCG                                                                <  SRR4302542.6031324/94‑1 (MQ=60)
                               GAGTGCAGCGTCTTCCATGGTGTGTTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTCTGCCGATCAACGTCACATACTGAAATGATGCG                                                                >  SRR4302542.6224196/1‑94 (MQ=60)
                                AGTGCAGCGTCTTCCATGGTGTGTTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTCTGCCGATCAACGTCACATACTGAAATGATGCGT                                                               <  SRR4302542.4488114/94‑1 (MQ=60)
                                  TGCAGCGTCTTCCATGGTGTGTTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTCTGCCGATCAACGTCACATACTGAAATGATGCGTAA                                                             >  SRR4302542.2211043/1‑94 (MQ=60)
                                   GCAGCGTCTTCCATGGTGTGTTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTCTGCCGATCAACGTCACATACTGAAATGATGCGTAAA                                                            >  SRR4302542.2118623/1‑94 (MQ=60)
                                    CAGCGTCTTCCATGGTGTGTTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTCTGCCGATCAACGTCACATACTGAAATGATGCGTAAAA                                                           >  SRR4302542.755741/1‑94 (MQ=60)
                                    CAGCGTCTTCCATGGTGTGTTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTCCGCCGATCAACGTCACATACTGAAATGATGCGTAAAA                                                           <  SRR4302542.4502350/94‑1 (MQ=60)
                                     AGCGTCTTCCATGGTGTGTTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTCTGCCGATCAACGTCACATACTGAAATGATGCGTAAAAA                                                          >  SRR4302542.6184969/1‑94 (MQ=60)
                                      GCGTCTTCCATGGTGTGTTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTCTGCCGATCAACGTCACATACTGAAATGATGCGTAAAAAC                                                         >  SRR4302542.2610976/1‑94 (MQ=60)
                                       CGTCTTCCATGGTGTGTTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTCTGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACC                                                        >  SRR4302542.4192309/1‑94 (MQ=60)
                                        GTCTTCCATGGTGTGTTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTCTGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCT                                                       >  SRR4302542.6700224/1‑94 (MQ=60)
                                        GTCTTCCATGGTGTGTTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTCTGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCT                                                       >  SRR4302542.3983276/1‑94 (MQ=60)
                                        GTCTTCCATGGTGTGTTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTCTGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCT                                                       >  SRR4302542.6307642/1‑94 (MQ=60)
                                          CTTCCATGGTGTGTTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTCTGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTT                                                     <  SRR4302542.5833655/94‑1 (MQ=60)
                                          CTTCCATGGTGTGTTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTCTGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTT                                                     >  SRR4302542.1642011/1‑94 (MQ=60)
                                          CTTCCATGGTGTGTTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTCTGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTT                                                     <  SRR4302542.1412339/94‑1 (MQ=60)
                                           TTCCATGGTGTGTTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTCTGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTG                                                    <  SRR4302542.571724/94‑1 (MQ=60)
                                           TTCCATGGTGTGTTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTCTGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTG                                                    >  SRR4302542.5485073/1‑94 (MQ=60)
                                           TTCCATGGTGTGTTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTCTGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTG                                                    <  SRR4302542.837473/94‑1 (MQ=60)
                                             CCATGGTGTGTTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTCTGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCG                                                  <  SRR4302542.1203727/94‑1 (MQ=60)
                                             CCATGGTGTGTTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTCTGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCG                                                  <  SRR4302542.6500441/94‑1 (MQ=60)
                                             CCATGGTGTGTTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTCCGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCG                                                  >  SRR4302542.7365735/1‑94 (MQ=60)
                                              CATGGTGTGTTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTCTGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGC                                                 >  SRR4302542.796719/1‑94 (MQ=60)
                                              CATGGTGTGTTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTCTGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGC                                                 >  SRR4302542.2514133/1‑94 (MQ=60)
                                              CATGGTGTGTTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTCTGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGC                                                 >  SRR4302542.930078/1‑94 (MQ=60)
                                              CATGGTGTGTTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTCCGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGC                                                 >  SRR4302542.4723493/1‑94 (MQ=60)
                                               ATGGTGTGTTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTCTGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCC                                                <  SRR4302542.6021644/94‑1 (MQ=60)
                                               ATGGTGTGTTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTCTGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCC                                                <  SRR4302542.3042038/94‑1 (MQ=60)
                                               ATGGTGTGTTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTCCGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCC                                                >  SRR4302542.2806507/1‑94 (MQ=60)
                                                TGGTGTGTTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTCTGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCC                                               <  SRR4302542.2160612/94‑1 (MQ=60)
                                                TGGTGTGTTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTCTGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCC                                               >  SRR4302542.4241113/1‑94 (MQ=60)
                                                TGGTGTGTTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTCTGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCC                                               <  SRR4302542.2984971/94‑1 (MQ=60)
                                                 GGTGTGTTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTCTGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCT                                              <  SRR4302542.2167633/94‑1 (MQ=60)
                                                 GGTGTGTTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTCTGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCT                                              <  SRR4302542.2614323/94‑1 (MQ=60)
                                                  GTGTGTTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTCTGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTT                                             >  SRR4302542.3790333/1‑94 (MQ=60)
                                                  GTGTGTTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTCTGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTT                                             >  SRR4302542.5940595/1‑94 (MQ=60)
                                                   TGTGTTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTCCGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTC                                            >  SRR4302542.5785385/1‑94 (MQ=60)
                                                    GTGTTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTCTGTCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCA                                           <  SRR4302542.3760922/94‑1 (MQ=60)
                                                    GTGTTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTCCGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCA                                           <  SRR4302542.6481924/94‑1 (MQ=60)
                                                    GTGTTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTCCGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCA                                           >  SRR4302542.7189964/1‑94 (MQ=60)
                                                     TGTTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTCTGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAA                                          >  SRR4302542.4288145/1‑94 (MQ=60)
                                                      GTTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTCTGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAAC                                         >  SRR4302542.790748/1‑94 (MQ=60)
                                                      GTTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTCTGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAAC                                         <  SRR4302542.21524/94‑1 (MQ=60)
                                                      GTTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTCCGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAAC                                         <  SRR4302542.4993129/94‑1 (MQ=60)
                                                       TTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTCTGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACG                                        <  SRR4302542.3393197/94‑1 (MQ=60)
                                                        TGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTCTGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGG                                       >  SRR4302542.7438948/1‑94 (MQ=60)
                                                        TGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTCTGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGG                                       >  SRR4302542.3200295/1‑94 (MQ=60)
                                                         GTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTCTGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGC                                      >  SRR4302542.6993041/1‑94 (MQ=60)
                                                          TAAAACCACGACAACCAGTAGGGCTAGCAGTTGTCTGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCA                                     <  SRR4302542.426972/94‑1 (MQ=60)
                                                          TAAAACCACGACAACCAGTAGGGCTAGCAGTTGTCTGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCA                                     <  SRR4302542.6282322/94‑1 (MQ=60)
                                                            AAACCACGACAACCAGTAGGGCTAGCAGTTGTCTGCCGATCAACGTCACATTCTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAAC                                   <  SRR4302542.4210651/94‑1 (MQ=60)
                                                            AAACCACGACAACCAGTAGGGCTAGCAGTTGTCTGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAAC                                   <  SRR4302542.148216/94‑1 (MQ=60)
                                                             AACCACGACAACCAGTAGGGCTAGCAGTTGTCTGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACT                                  <  SRR4302542.7359037/94‑1 (MQ=60)
                                                             AACCACGACAACCAGTAGGGCTAGCAGTTGTCTGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACT                                  >  SRR4302542.819484/1‑94 (MQ=60)
                                                               CCACGACAACCAGTAGGGCTAGCAGTTGTCTGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTC                                >  SRR4302542.2228822/1‑94 (MQ=60)
                                                               CCACGACAACCAGTAGGGCTAGCAGTTGTCTGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTC                                >  SRR4302542.1684845/1‑94 (MQ=60)
                                                                CACGACAACCAGTAGGGCTAGCAGTTGTCTGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCA                               >  SRR4302542.2428040/1‑94 (MQ=60)
                                                                CACGACAACCAGTAGGGCTAGCAGTTGTCTGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCA                               >  SRR4302542.3866778/1‑94 (MQ=60)
                                                                CACGACAACCAGTAGGGCTAGCAGTTGTCTGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCA                               <  SRR4302542.1308444/94‑1 (MQ=60)
                                                                   GACAACCAGTAGGGCTAGCAGTTGTCTGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTC                            >  SRR4302542.2796838/1‑94 (MQ=60)
                                                                   GACAACCAGTAGGGCTAGCAGTTGTCCGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTC                            >  SRR4302542.2827697/1‑94 (MQ=60)
                                                                     CAACCAGTAGGGCTAGCAGTTGTCTGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCAT                          <  SRR4302542.7515317/94‑1 (MQ=60)
                                                                     CAACCAGTAGGGCTAGCAGTTGTCTGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCAT                          <  SRR4302542.5002010/94‑1 (MQ=60)
                                                                     CAACCAGTAGGGCTAGCAGTTGTCTGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCAT                          >  SRR4302542.7083657/1‑94 (MQ=60)
                                                                     CAACCAGTAGGGCTAGCAGTTGTCTGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCAT                          >  SRR4302542.4579969/1‑94 (MQ=60)
                                                                     CAACCAGTAGGGCTAGCAGTTGTCTGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCAT                          >  SRR4302542.1924037/1‑94 (MQ=60)
                                                                      AACCAGTAGGGCTAGCAGTTGTCTGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATA                         >  SRR4302542.5265065/1‑94 (MQ=60)
                                                                       ACCAGTAGGGCTAGCAGTTGTCTGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATAT                        >  SRR4302542.6066443/1‑94 (MQ=60)
                                                                        CCAGTAGGGCTAGCAGTTGTCTGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATA                       <  SRR4302542.118783/94‑1 (MQ=60)
                                                                         CAGTAGGGCTAGCAGTTGTCTGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAG                      <  SRR4302542.4461564/94‑1 (MQ=60)
                                                                         CAGTAGGGCTAGCAGTTGTCTGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAG                      <  SRR4302542.4527256/94‑1 (MQ=60)
                                                                         CAGTAGGGCTAGCAGTTGTCTGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAG                      <  SRR4302542.2158018/94‑1 (MQ=60)
                                                                         CAGTAGGGCTAGCAGTTGTCTGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAG                      <  SRR4302542.7385171/94‑1 (MQ=60)
                                                                         CAGTAGGGCTAGCAGTTGTCCGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAG                      >  SRR4302542.6315605/1‑94 (MQ=60)
                                                                          AGTAGGGCTAGCAGTTGTCTGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGG                     >  SRR4302542.3169415/1‑94 (MQ=60)
                                                                          AGTAGGGCTAGCAGTTGTCTGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGG                     <  SRR4302542.1785982/94‑1 (MQ=60)
                                                                           GTAGGGCTAGCAGTTGTCTGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGT                    >  SRR4302542.6891058/1‑94 (MQ=60)
                                                                            TAGGGCTAGCAGTTGTCTGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTG                   <  SRR4302542.4897806/94‑1 (MQ=60)
                                                                             AGGGCTAGCAGTTGTCTGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGG                  >  SRR4302542.3729209/1‑94 (MQ=60)
                                                                              GGGCTAGCAGTTGTCTGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGC                 >  SRR4302542.5107981/1‑94 (MQ=60)
                                                                                GCTAGCAGTTGTCTGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGC               >  SRR4302542.3189112/1‑94 (MQ=60)
                                                                                 CTAGCAGTTGTCTGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCT              >  SRR4302542.6350614/1‑94 (MQ=60)
                                                                                 CTAGCAGTTGTCTGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCT              >  SRR4302542.3389607/1‑94 (MQ=60)
                                                                                  TAGCAGTTGTCTGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTG             >  SRR4302542.7245764/1‑94 (MQ=60)
                                                                                    GCAGTTGTCTGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGT           >  SRR4302542.5922062/1‑94 (MQ=60)
                                                                                       GTTGTCTGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGCC        <  SRR4302542.3215345/94‑1 (MQ=60)
                                                                                       GTTGTCCGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGCC        >  SRR4302542.5113175/1‑94 (MQ=60)
                                                                                          GTCTGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGCCATA     >  SRR4302542.7594579/1‑94 (MQ=60)
                                                                                           TCTGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGCCATAT    <  SRR4302542.1980146/94‑1 (MQ=60)
                                                                                           TCTGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGCCATAT    >  SRR4302542.2720982/1‑94 (MQ=60)
                                                                                           TCCGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGCCATAT    <  SRR4302542.3235722/94‑1 (MQ=60)
                                                                                           TCCGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGCCATAT    <  SRR4302542.1109203/94‑1 (MQ=60)
                                                                                            CTGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGCCATATT   >  SRR4302542.7341500/1‑94 (MQ=60)
                                                                                            CTGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGCCATATT   <  SRR4302542.3194718/94‑1 (MQ=60)
                                                                                            CCGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGCCATATT   >  SRR4302542.3909751/1‑94 (MQ=60)
                                                                                             TGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGCCATATTC  <  SRR4302542.1870334/94‑1 (MQ=60)
                                                                                             TGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGCCATATTC  >  SRR4302542.5692611/1‑94 (MQ=60)
                                                                                             |                                                                                             
ACGCGAAAGGAATACGCCATCCCCACTCTCTGAGTGCAGCGTCTTCCATGGTGTGTTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTCCGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGCCATATTC  >  NC_000913/2723160‑2723346

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑