Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	1061	294.1	15065038	98.6%	14854127	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,781,020	+G	intergenic (‑144/+65)	ypjA ← / ← b2648	putative ATP‑binding component of a transport system/hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,781,019	1	.	G	99.6%	781.1 / ‑3.9	256	intergenic (‑143/+66)	ypjA/b2648	putative ATP‑binding component of a transport system/hypothetical protein
Reads supporting (aligned to +/- strand): ref base . (1/0); new base G (115/140); total (116/140)
Fisher's exact test for biased strand distribution p-value = 4.53e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.81e-01

Only 100 of 282 total aligned reads displayed.

GGCATTTTTTAAACGCAGTGCGCTGAAGTGTGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGA‑GCCGTTGCCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAACTTTGAGATCG  >  NC_000913/2780927‑2781106

ggCATTTTTTAAACGCAGTGCGCTGAAGTGTGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGa                                                                                          <  1:6521418/93‑1 (MQ=255)

ggCATTTTTTAAACGCAGTGCGCTGAAGTGTGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGa                                                                                          <  1:7394702/93‑1 (MQ=255)

 gCATTTTTTAAACGCAGTGCGCTGAAGTGTGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGA‑g                                                                                        >  1:6722570/1‑93 (MQ=255)

 gCATTTTTTAAACGCAGTGCGCTGAAGTGTGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGA‑g                                                                                        <  2:5085217/93‑1 (MQ=255)

 gCATTTTTTAAACGCAGTGCGCTGAAGTGTGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGA‑g                                                                                        >  2:575444/1‑93 (MQ=255)

   aTTTTTTAAACGCAGTGCGCTGAAGTGTGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGA‑ggc                                                                                      <  2:6822824/93‑3 (MQ=255)

        ttAAACGCAGTGCGCTGAAGTGTGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTg                                                                                  >  2:4959850/1‑93 (MQ=255)

        ttAAACGCAGTGCGCTGAAGTGTGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTg                                                                                  <  1:7073989/93‑1 (MQ=255)

         tAAACGCAGTGCGCTGAAGTGTGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTgg                                                                                 <  1:6962021/93‑2 (MQ=255)

              gCAGTGCGCTGAAGTGTGGTTGGATAAAAAAGTCAATCCATTCATGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCaaaa                                                                            >  1:4717845/1‑93 (MQ=25)

              gCAGTGCGCTGAAGTGTGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCaaaa                                                                            >  1:1317271/1‑93 (MQ=255)

              gCAGTGCGCTGAAGTGTGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCaaaa                                                                            <  2:4375483/93‑1 (MQ=37)

              gCAGTGCGCTGAAGTGTGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCaaaa                                                                            >  1:7235857/1‑93 (MQ=255)

              gCAGTGCGCTGAAGTGTGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCaaaa                                                                            <  1:389030/93‑1 (MQ=25)

                  tGCGCTGAAGTGTGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCaaaataaa                                                                        <  1:2279439/93‑1 (MQ=255)

                   gcgcTGAAGTGTGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCaaaataaaa                                                                       >  1:2795456/1‑93 (MQ=255)

                   gcgcTGAAGTGTGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCaaaataaaa                                                                       >  1:2686668/1‑93 (MQ=255)

                      cTGAAGTGTGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGa                                                                    >  1:6063818/1‑93 (MQ=255)

                      cTGAAGTGTGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGa                                                                    <  1:2540144/93‑1 (MQ=25)

                       tGAAGTGTGGGTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGAt                                                                   <  1:5526631/93‑1 (MQ=25)

                        gAAGTGTGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGAtt                                                                  >  2:72345/1‑93 (MQ=255)

                         aaGTGTGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGAttt                                                                 >  2:6456645/1‑93 (MQ=255)

                          aGTGTGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTa                                                                <  2:2986372/93‑1 (MQ=37)

                          aGTGTGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTa                                                                <  2:2412404/93‑1 (MQ=37)

                          aGTGTGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTa                                                                <  1:953890/93‑1 (MQ=37)

                            tgtgGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACa                                                              <  1:3943126/93‑1 (MQ=37)

                            tgtgGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACa                                                              >  2:5856580/1‑93 (MQ=255)

                            tgtgGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACa                                                              <  1:4888797/93‑1 (MQ=37)

                             gtgGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACAt                                                             <  2:1148768/93‑1 (MQ=37)

                              tgGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATa                                                            >  2:4118500/1‑93 (MQ=255)

                              tgGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATa                                                            <  1:2625773/93‑1 (MQ=37)

                                gTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAAt                                                          <  2:144805/93‑1 (MQ=37)

                                gTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAAt                                                          <  1:6140167/93‑1 (MQ=37)

                                  tGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCg                                                        >  2:1964527/1‑93 (MQ=255)

                                   ggATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGt                                                       <  1:1833650/93‑1 (MQ=38)

                                    gATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGtt                                                      <  2:3255453/93‑1 (MQ=38)

                                     aTAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGttt                                                     <  2:1822658/93‑1 (MQ=38)

                                      tAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTc                                                    <  2:3481514/93‑1 (MQ=38)

                                      tAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTc                                                    <  1:3705622/93‑1 (MQ=38)

                                           aaGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCtgatg                                               >  1:1074025/1‑93 (MQ=255)

                                            aGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCtgatga                                              <  1:3394792/93‑1 (MQ=37)

                                             gTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGaa                                             >  1:6280173/1‑93 (MQ=255)

                                             gTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGaa                                             <  1:2980143/93‑1 (MQ=37)

                                                aaTCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAatat                                          <  2:161979/93‑1 (MQ=37)

                                                  tCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATAtct                                        <  1:5803134/93‑1 (MQ=25)

                                                    cATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATAtcttc                                      <  1:5930810/93‑1 (MQ=25)

                                                    cATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATAtcttc                                      <  1:4169650/93‑1 (MQ=25)

                                                    cATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATAtcttc                                      <  1:2438403/93‑1 (MQ=25)

                                                    cATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATAtcttc                                      <  1:2584909/93‑1 (MQ=25)

                                                    cATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATAtcttc                                      >  2:2769393/1‑93 (MQ=255)

                                                     aTTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATAtcttct                                     >  2:3171383/1‑93 (MQ=255)

                                                     aTTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATAtcttct                                     >  1:3452702/1‑93 (MQ=255)

                                                     aTTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATAtcttct                                     >  2:3843282/1‑93 (MQ=255)

                                                     aTTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATAtcttct                                     >  2:6539760/1‑93 (MQ=255)

                                                         aGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTc                                 >  2:5379182/1‑93 (MQ=255)

                                                         aGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTc                                 >  1:5592129/1‑93 (MQ=255)

                                                          ggAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTca                                <  2:2304747/93‑1 (MQ=21)

                                                            aaaTACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTcaca                              >  2:2179800/1‑93 (MQ=21)

                                                             aaTACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATCTCTTCTGCTCACAt                             <  2:5190939/93‑1 (MQ=21)

                                                             aaTACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACAt                             >  2:1216520/1‑93 (MQ=18)

                                                              aTACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATa                            <  2:3354421/93‑1 (MQ=21)

                                                              aTACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATa                            <  2:6995390/93‑1 (MQ=21)

                                                              aTACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATa                            <  1:217909/93‑1 (MQ=21)

                                                                aCGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATaaa                          <  2:447987/93‑1 (MQ=18)

                                                                 cGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATaaaa                         >  1:4848075/1‑93 (MQ=255)

                                                                   ggCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAAt                       <  1:3338456/93‑1 (MQ=18)

                                                                    gCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATc                      >  1:7179431/1‑93 (MQ=17)

                                                                       tATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATcaca                   >  1:1468588/1‑93 (MQ=18)

                                                                       tATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATcaca                   >  2:5493746/1‑93 (MQ=18)

                                                                       tATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATcaca                   >  2:7050942/1‑93 (MQ=18)

                                                                       tATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATcaca                   >  2:7361811/1‑93 (MQ=18)

                                                                        aTTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATcacac                  >  1:425155/1‑93 (MQ=17)

                                                                        aTTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATcacac                  <  2:1264884/93‑1 (MQ=17)

                                                                         ttcttttcttTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATcacaca                 >  2:6155391/1‑93 (MQ=17)

                                                                         ttcttttcttTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATcacaca                 <  1:5373711/93‑1 (MQ=17)

                                                                         ttcttttcttTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATcacaca                 <  1:7526083/93‑1 (MQ=17)

                                                                          tcttttctttCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACaa                <  2:5429850/93‑1 (MQ=17)

                                                                          tcttttctttCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACaa                >  1:4052465/1‑93 (MQ=17)

                                                                           cttttctttCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAAt               >  1:1132893/1‑93 (MQ=17)

                                                                           cttttctttCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAAt               >  1:4991610/1‑93 (MQ=14)

                                                                              ttctttcGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAAc            >  2:3586749/1‑93 (MQ=255)

                                                                              ttctttcGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAAc            <  2:2596576/93‑1 (MQ=255)

                                                                               tctttcGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAACt           >  2:4873352/1‑93 (MQ=17)

                                                                                ctttcGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAACtt          <  2:7490709/93‑1 (MQ=17)

                                                                                ctttcGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAACtt          <  2:3753267/93‑1 (MQ=17)

                                                                                ctttcGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAACtt          <  2:2877816/93‑1 (MQ=255)

                                                                                ctttcGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAACtt          <  2:1683969/93‑1 (MQ=255)

                                                                                ctttcGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAACtt          >  1:2040991/1‑93 (MQ=17)

                                                                                 tttcGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAACttt         <  2:4534698/93‑1 (MQ=17)

                                                                                 tttcGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAACttt         >  2:1579652/1‑93 (MQ=17)

                                                                                   tcGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAACTTTga       <  1:166868/93‑1 (MQ=255)

                                                                                   tcGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAACTTTga       <  1:6879974/93‑1 (MQ=255)

                                                                                   tcGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAACTTTga       <  1:6092420/93‑1 (MQ=255)

                                                                                    cGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAACTTTgag      <  1:4577329/93‑1 (MQ=255)

                                                                                     gACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAACTTTgaga     >  2:798739/1‑93 (MQ=18)

                                                                                     gACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAACTTTgaga     >  2:6209044/1‑93 (MQ=18)

                                                                                     gACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAACTTTgaga     >  2:6403739/1‑93 (MQ=18)

                                                                                     gACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAACTTTgaga     <  2:3972283/93‑1 (MQ=255)

                                                                                       cAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAACTTTGAGATc   >  1:5838953/1‑93 (MQ=18)

                                                                                        aaTGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAACTTTGAGATCg  >  2:4700834/1‑93 (MQ=18)

GGCATTTTTTAAACGCAGTGCGCTGAAGTGTGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGA‑GCCGTTGCCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAACTTTGAGATCG  >  NC_000913/2780927‑2781106

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

GGCATTTTTTAAACGCAGTGCGCTGAAGTGTGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGA‑GCCGTTGCCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAACTTTGAGATCGCAGATT  >  NC_000913/2780927‑2781112

GGCATTTTTTAAACGCAGTGCGCTGAAGTGTGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGA‑G                                                                                              <  SRR4302542.3966773/94‑1 (MQ=60)

GGCATTTTTTAAACGCAGTGCGCTGAAGTGTGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGA‑G                                                                                              <  SRR4302542.6156971/94‑1 (MQ=60)

GGCATTTTTTAAACGCAGTGCGCTGAAGTGTGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGA‑G                                                                                              <  SRR4302542.6585679/94‑1 (MQ=60)

GGCATTTTTTAAACGCAGTGCGCTGAAGTGTGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGA‑G                                                                                              >  SRR4302542.6788765/1‑94 (MQ=60)

GGCATTTTTTAAACGCAGTGCGCTGAAGTGTGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGA‑G                                                                                              <  SRR4302542.7467079/94‑1 (MQ=60)

   ATTTTTTAAACGCAGTGCGCTGAAGTGTGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGA‑Ggcc                                                                                           >  SRR4302542.4679768/1‑91 (MQ=60)

   ATTTTTTAAACGCAGTGCGCTGAAGTGTGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGA‑Ggcc                                                                                           <  SRR4302542.6135231/94‑4 (MQ=60)

   ATTTTTTAAACGCAGTGCGCTGAAGTGTGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGA‑Ggcc                                                                                           <  SRR4302542.2966603/94‑4 (MQ=60)

      TTTTAAACGCAGTGCGCTGAAGTGTGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTT                                                                                         >  SRR4302542.4189625/1‑94 (MQ=60)

      TTTTAAACGCAGTGCGCTGAAGTGTGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTT                                                                                         <  SRR4302542.4077796/94‑1 (MQ=60)

       TTTAAACGCAGTGCGCTGAAGTGTGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTG                                                                                        >  SRR4302542.7122164/1‑94 (MQ=60)

        TTAAACGCAGTGCGCTGAAGTGTGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGA‑Ggccgttgg                                                                                      <  SRR4302542.7143415/94‑9 (MQ=60)

        TTAAACGCAGTGCGCTGAAGTGTGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGA‑Ggccgttgg                                                                                      <  SRR4302542.2251012/94‑9 (MQ=60)

         TAAACGCAGTGCGCTGAAGTGTGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGC                                                                                      <  SRR4302542.7030423/94‑1 (MQ=60)

         TAAACGAAGTGGGCTGAAGGGTGGTTGAATAAAAAAGTCAATCCATTCAGGAAATCCGGGCTTATTCTTTTCTTTCGACAATGAGGCCGTTGGC                                                                                      <  SRR4302542.2373281/94‑1 (MQ=60)

             CGCAGTGCGCTGAAGTGTGGTTGGATAAAAAAGTCAATCCATTCATGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAA                                                                                  >  SRR4302542.4763837/1‑94 (MQ=60)

             CGCAGTGCGCTGAAGTGTGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAA                                                                                  >  SRR4302542.1328623/1‑94 (MQ=60)

             CGCAGTGCGCTGAAGTGTGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAA                                                                                  >  SRR4302542.7306839/1‑94 (MQ=60)

              GCAGTGCGCTGAAGTGTGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAAT                                                                                 >  SRR4302542.275174/1‑94 (MQ=60)

              GCAGTGCGCTGAAGTGTGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAAT                                                                                 >  SRR4302542.3820649/1‑94 (MQ=60)

              GCAGTGCGCTGAAGTGTGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAAT                                                                                 <  SRR4302542.392399/94‑1 (MQ=60)

              GCAGTGCGCTGAAGTGTGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAAT                                                                                 <  SRR4302542.1398105/94‑1 (MQ=60)

                  TGCGCTGAAGTGTGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAA                                                                             <  SRR4302542.2299029/94‑1 (MQ=60)

                  TGCGCTGAAGTGTGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAA                                                                             >  SRR4302542.2712572/1‑94 (MQ=60)

                  TGCGCTGAAGTGTGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAA                                                                             >  SRR4302542.2822353/1‑94 (MQ=60)

                  TGCGCTGAAGTGTGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAA                                                                             <  SRR4302542.265528/94‑1 (MQ=60)

                   GCGCTGAAGTGTGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAAT                                                                            <  SRR4302542.7131097/94‑1 (MQ=60)

                    CGCTGAAGTGTGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATG                                                                           <  SRR4302542.423353/94‑1 (MQ=60)

                     GCTGAAGTGTGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGA                                                                          <  SRR4302542.5285038/94‑1 (MQ=60)

                     GCTGAAGTGTGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGA                                                                          >  SRR4302542.6122552/1‑94 (MQ=60)

                     GCTGAAGTGTGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGA                                                                          >  SRR4302542.7571475/1‑94 (MQ=60)

                      CTGAAGTGTGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGAT                                                                         <  SRR4302542.2564772/94‑1 (MQ=60)

                      CTGAAGTGTGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGAT                                                                         >  SRR4302542.606562/1‑94 (MQ=60)

                       TGAAGTGTGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATT                                                                        <  SRR4302542.3142462/94‑1 (MQ=60)

                       TGAAGTGTGGGTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATT                                                                        <  SRR4302542.5580348/94‑1 (MQ=60)

                        GAAGTGTGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTT                                                                       <  SRR4302542.611238/94‑1 (MQ=60)

                         AAGTGTGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTA                                                                      <  SRR4302542.5575998/94‑1 (MQ=60)

                          AGTGTGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTAC                                                                     <  SRR4302542.962078/94‑1 (MQ=60)

                           GTGTGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACA                                                                    >  SRR4302542.2597577/1‑94 (MQ=60)

                            TGTGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACAT                                                                   <  SRR4302542.3982075/94‑1 (MQ=60)

                            TGTGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACAT                                                                   <  SRR4302542.4936353/94‑1 (MQ=60)

                            TGTGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACAT                                                                   <  SRR4302542.7260915/94‑1 (MQ=60)

                              TGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAA                                                                 <  SRR4302542.2651143/94‑1 (MQ=60)

                                GTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATC                                                               <  SRR4302542.6199580/94‑1 (MQ=60)

                                GTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATC                                                               >  SRR4302542.5926978/1‑94 (MQ=60)

                                 TTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCG                                                              <  SRR4302542.627384/94‑1 (MQ=60)

                                   GGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTT                                                            <  SRR4302542.7029962/94‑1 (MQ=60)

                                   GGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTT                                                            >  SRR4302542.3027449/1‑94 (MQ=60)

                                   GGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTT                                                            <  SRR4302542.1849531/94‑1 (MQ=60)

                                    GATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTT                                                           <  SRR4302542.904029/94‑1 (MQ=60)

                                     ATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTC                                                          <  SRR4302542.6560345/94‑1 (MQ=60)

                                     ATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTC                                                          <  SRR4302542.5429864/94‑1 (MQ=60)

                                     ATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTC                                                          <  SRR4302542.2688789/94‑1 (MQ=60)

                                      TAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCT                                                         <  SRR4302542.3742291/94‑1 (MQ=60)

                                       AAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTG                                                        >  SRR4302542.5880359/1‑94 (MQ=60)

                                       AAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTG                                                        <  SRR4302542.3466688/94‑1 (MQ=60)

                                         AAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGAT                                                      <  SRR4302542.2613399/94‑1 (MQ=60)

                                         AAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGAT                                                      >  SRR4302542.734667/1‑94 (MQ=60)

                                          AAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATG                                                     >  SRR4302542.1083258/1‑94 (MQ=60)

                                          AAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATG                                                     <  SRR4302542.248598/94‑1 (MQ=60)

                                           AAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGA                                                    <  SRR4302542.5038239/94‑1 (MQ=60)

                                            AGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAA                                                   >  SRR4302542.150022/1‑94 (MQ=60)

                                            AGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAA                                                   >  SRR4302542.6342060/1‑94 (MQ=60)

                                            AGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAA                                                   >  SRR4302542.7271415/1‑94 (MQ=60)

                                            AGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAA                                                   <  SRR4302542.3426642/94‑1 (MQ=60)

                                             GTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAAT                                                  <  SRR4302542.1108072/94‑1 (MQ=60)

                                             GTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAAT                                                  <  SRR4302542.3008675/94‑1 (MQ=60)

                                               CAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATAT                                                >  SRR4302542.5635135/1‑94 (MQ=60)

                                               CAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATAT                                                >  SRR4302542.5928607/1‑94 (MQ=60)

                                               CAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATAT                                                >  SRR4302542.3926785/1‑94 (MQ=60)

                                                AATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATC                                               >  SRR4302542.6710808/1‑94 (MQ=60)

                                                AATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATC                                               >  SRR4302542.2177674/1‑94 (MQ=60)

                                                  TCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTT                                             <  SRR4302542.5859450/94‑1 (MQ=60)

                                                   CCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTC                                            <  SRR4302542.6883388/94‑1 (MQ=60)

                                                   CCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTC                                            <  SRR4302542.2200074/94‑1 (MQ=60)

                                                   CCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTC                                            <  SRR4302542.6537749/94‑1 (MQ=60)

                                                    CATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCT                                           >  SRR4302542.3485001/1‑94 (MQ=60)

                                                    CATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCT                                           <  SRR4302542.3373206/94‑1 (MQ=60)

                                                    CATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCT                                           <  SRR4302542.5988269/94‑1 (MQ=60)

                                                    CATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCT                                           <  SRR4302542.2609951/94‑1 (MQ=60)

                                                    CATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCT                                           <  SRR4302542.2462130/94‑1 (MQ=60)

                                                    CATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCT                                           <  SRR4302542.4210774/94‑1 (MQ=60)

                                                    CATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCT                                           <  SRR4302542.287853/94‑1 (MQ=60)

                                                     ATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTG                                          <  SRR4302542.1745342/94‑1 (MQ=60)

                                                     ATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTG                                          <  SRR4302542.2058420/94‑1 (MQ=60)

                                                      TTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGC                                         >  SRR4302542.4505853/1‑94 (MQ=60)

                                                      TTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGC                                         <  SRR4302542.694955/94‑1 (MQ=60)

                                                        CAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTC                                       >  SRR4302542.5646467/1‑94 (MQ=60)

                                                          GGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCAC                                     <  SRR4302542.4774776/94‑1 (MQ=60)

                                                           GAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACA                                    >  SRR4302542.3735575/1‑94 (MQ=60)

                                                            AAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACAT                                   <  SRR4302542.1575064/94‑1 (MQ=60)

                                                            AAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACAT                                   <  SRR4302542.4233194/94‑1 (MQ=60)

                                                              ATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAA                                 <  SRR4302542.1716263/94‑1 (MQ=60)

                                                              ATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAA                                 >  SRR4302542.6195296/1‑94 (MQ=60)

                                                              ATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAA                                 <  SRR4302542.219789/94‑1 (MQ=60)

                                                               TACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAA                                <  SRR4302542.4069756/94‑1 (MQ=60)

                                                                ACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAA                               >  SRR4302542.4895281/1‑94 (MQ=60)

                                                                ACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAA                               <  SRR4302542.925917/94‑1 (MQ=60)

                                                                 CGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAA                              <  SRR4302542.4414120/94‑1 (MQ=60)

                                                                  GGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAAT                             <  SRR4302542.2430387/94‑1 (MQ=60)

                                                                  GGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAAT                             <  SRR4302542.4712974/94‑1 (MQ=60)

                                                                   GGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATC                            <  SRR4302542.4887875/94‑1 (MQ=60)

                                                                   GGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATC                            >  SRR4302542.575034/1‑94 (MQ=60)

                                                                   GGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATC                            <  SRR4302542.3369877/94‑1 (MQ=60)

                                                                   GGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATC                            >  SRR4302542.7249849/1‑94 (MQ=60)

                                                                    GCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCA                           <  SRR4302542.1149656/94‑1 (MQ=60)

                                                                      GTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACA                         <  SRR4302542.3988440/94‑1 (MQ=60)

                                                                      GTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACA                         >  SRR4302542.1481266/1‑94 (MQ=60)

                                                                       TATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACAC                        >  SRR4302542.428797/1‑94 (MQ=59)

                                                                        ATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACA                       >  SRR4302542.6132442/1‑94 (MQ=56)

                                                                         TTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAA                      <  SRR4302542.7600074/94‑1 (MQ=54)

                                                                         TTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAA                      <  SRR4302542.5425927/94‑1 (MQ=54)

                                                                         TTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAA                      >  SRR4302542.4092430/1‑94 (MQ=54)

                                                                         TTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAA                      <  SRR4302542.7145259/94‑1 (MQ=54)

                                                                          TCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAAT                     <  SRR4302542.3451218/94‑1 (MQ=52)

                                                                          TCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAAT                     >  SRR4302542.5040094/1‑94 (MQ=52)

                                                                          TCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAAT                     >  SRR4302542.1142590/1‑94 (MQ=52)

                                                                          TCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAAT                     >  SRR4302542.7344881/1‑94 (MQ=52)

                                                                            TTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAA                   >  SRR4302542.2489367/1‑94 (MQ=47)

                                                                             TTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAAC                  >  SRR4302542.3942018/1‑94 (MQ=47)

                                                                               TCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAACTT                >  SRR4302542.2058639/1‑94 (MQ=47)

                                                                                CTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAACTTT               <  SRR4302542.5731804/94‑1 (MQ=49)

                                                                                CTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAACTTT               <  SRR4302542.7524901/94‑1 (MQ=49)

                                                                                 TTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAACTTTG              <  SRR4302542.1263854/94‑1 (MQ=52)

                                                                                   TCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAACTTTGAG            <  SRR4302542.168283/94‑1 (MQ=56)

                                                                                   TCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAACTTTGAG            <  SRR4302542.6947553/94‑1 (MQ=56)

                                                                                   TCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAACTTTGAG            <  SRR4302542.6151396/94‑1 (MQ=56)

                                                                                    CGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAACTTTGAGA           <  SRR4302542.4622110/94‑1 (MQ=59)

                                                                                     GACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAACTTTGAGAT          >  SRR4302542.2150331/1‑94 (MQ=60)

                                                                                     GACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAACTTTGAGAT          >  SRR4302542.1348410/1‑94 (MQ=60)

                                                                                      ACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAACTTTGAGATC         <  SRR4302542.2609466/94‑1 (MQ=60)

                                                                                      ACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAACTTTGAGATC         >  SRR4302542.5895597/1‑94 (MQ=60)

                                                                                      ACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAACTTTGAGATC         <  SRR4302542.6831447/94‑1 (MQ=60)

                                                                                      ACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAACTTTGAGATC         <  SRR4302542.4935215/94‑1 (MQ=60)

                                                                                      ACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAACTTTGAGATC         <  SRR4302542.5608320/94‑1 (MQ=60)

                                                                                       CAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAACTTTGAGATCG        >  SRR4302542.6670650/1‑94 (MQ=60)

                                                                                       CAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAACTTTGAGATCG        <  SRR4302542.4965886/94‑1 (MQ=60)

                                                                                       CAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAACTTTGAGATCG        >  SRR4302542.2767627/1‑94 (MQ=60)

                                                                                       CAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAACTTTGAGATCG        >  SRR4302542.3599490/1‑94 (MQ=60)

                                                                                        AATGAGGCCGTTGGCAAAATAAAATGGTTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAACTTTGAGATCGC       >  SRR4302542.1880507/1‑94 (MQ=60)

                                                                                         ATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAACTTTGAGATCGCA      <  SRR4302542.271322/94‑1 (MQ=60)

                                                                                          TGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAACTTTGAGATCGCAG     >  SRR4302542.1649390/1‑94 (MQ=60)

                                                                                          TGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAACTTTGAGATCGCAG     >  SRR4302542.1905695/1‑94 (MQ=60)

                                                                                           gagGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAACTTTGAGATCGCAGA    >  SRR4302542.2555983/4‑94 (MQ=60)

                                                                                           gagGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAACTTTGAGATCGCAGA    >  SRR4302542.6675327/4‑94 (MQ=60)

                                                                                           gagGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAACTTTGAGATCGCAGA    >  SRR4302542.4663713/4‑94 (MQ=60)

                                                                                            agGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAACTTTGAGATCGCAGAT   <  SRR4302542.6287359/92‑1 (MQ=60)

                                                                                            agGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAACTTTGAGATCGCAGAT   <  SRR4302542.2172895/92‑1 (MQ=60)

                                                                                            G‑GCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAACTTTGAGATCGCAGATT  >  SRR4302542.3981880/1‑94 (MQ=60)

GGCATTTTTTAAACGCAGTGCGCTGAAGTGTGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGA‑GCCGTTGCCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAACTTTGAGATCGCAGATT  >  NC_000913/2780927‑2781112

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`