Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1061 294.1 15065038 98.6% 14854127 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,812,859 Δ1 bp intergenic (‑105/+46) ygaG ← / ← gshA hypothetical protein/gamma‑glutamate‑cysteine ligase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,812,8580C.99.6% 1249.8 / ‑3.8 272intergenic (‑104/+47)ygaG/gshAhypothetical protein/gamma‑glutamate‑cysteine ligase
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base . (162/109);  total (163/109)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01

Only 100 of 283 total aligned reads displayed.

CGGTAATTTTTTTAAAAATTTTCTGAACTCTTTCTTCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATTCCAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGTTTTTCCAGCCACACCGCAAACGGTTCGGTATC  >  NC_000913/2812766‑2812946
                                                                                            |                                                                                        
 ggTAATTTTTTTAAAAATTTTCTGAACTCTTTCTTCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATTca                                                                                         >  2:348814/1‑92 (MQ=255)
  nnaaTTTTTTTAAAAATTTTCTGAACTCTTTCTTCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATTcag                                                                                        <  2:1444748/91‑3 (MQ=255)
  gTAATTTTTTTAAAAATTTTCTGAACTCTTTCTTCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATTcag                                                                                        >  1:1588894/1‑91 (MQ=255)
   tAATTTTTTTAAAAATTTTCTGAACTCTTTCTTCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATTcaga                                                                                       <  2:7049510/93‑4 (MQ=255)
   tAATTTTTTTAAAAATTTTCTGAACTCTTTCTTCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATTcaga                                                                                       >  2:2563815/1‑90 (MQ=255)
     aTTTTTTTAAAAATTTTCTGAACTCTTTCTTCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATT‑CAgaga                                                                                    <  2:6502094/93‑1 (MQ=255)
     aTTTTTTTAAAAATTTTCTGAACTCTTTCTTCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATT‑CAgaga                                                                                    <  1:408979/93‑1 (MQ=255)
         ttttAAAAATTTTCTGAACTCTTTCTTCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAA‑TCCAGAGATGaa                                                                                >  1:3150959/1‑93 (MQ=255)
          tttAAAAATTTTCTGAACTCTTTCTTCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATT‑CAGAGATGaaa                                                                               >  1:2536829/1‑93 (MQ=255)
            tAAAAATTTTCTGAACTCTTTCTTCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATT‑CAGAGATGAAAtt                                                                             >  1:1155653/1‑93 (MQ=255)
             aaaaaTTTTCTGAACTCTTTCTTCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATT‑CAGAGATGAAAttt                                                                            <  1:7023307/93‑1 (MQ=255)
              aaaaTTTTCTGAACTCTTTCTTCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATT‑CAGAGATGAAAtttt                                                                           >  1:2381519/1‑93 (MQ=255)
              aaaaTTTTCTGAACTCTTTCTTCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATT‑CAGAGATGAAAtttt                                                                           <  1:4317639/93‑1 (MQ=255)
                aaTTTTCTGAACTCTTTCTTCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATT‑CAGAGATGAAATTTTgg                                                                         <  2:1071854/93‑1 (MQ=255)
                 aTTTTCTGAACTCTTTCTTCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATT‑CAGAGATGAAATTTTGGc                                                                        <  1:677160/93‑1 (MQ=255)
                 aTTTTCTGAACTCTTTCTTCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATT‑CAGAGATGAAATTTTGGc                                                                        >  1:5119841/1‑93 (MQ=255)
                 aTTTTCTGAACTCTTTCTTCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATT‑CAGAGATGAAATTTTGGc                                                                        >  2:5735066/1‑93 (MQ=255)
                  ttttCTGAACTCTTTCTTCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATT‑CAGAGATGAAATTTTGGcc                                                                       >  1:6103090/1‑93 (MQ=255)
                       tGAACTCTTTCTTCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATT‑CAGAGATGAAATTTTGGCCACTCa                                                                  >  2:4240693/1‑93 (MQ=255)
                        gAACTCTTTCTTCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATT‑CAGAGATGAAATTTTGGCCACTCAc                                                                 >  2:2423318/1‑93 (MQ=255)
                        gAACTCTTTCTTCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATT‑CAGAGATGAAATTTTGGCCACTCAc                                                                 >  1:6288296/1‑93 (MQ=255)
                        gAACTCTTTCTTCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATT‑CAGAGATGAAATTTTGGCCACTCAc                                                                 >  2:1836389/1‑93 (MQ=255)
                        gAACTCTTTCTTCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATT‑CAGAGATGAAATTTTGGCCACTCAc                                                                 >  2:2493626/1‑93 (MQ=255)
                         aaCTCTTTCTTCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATT‑CAGAGATGAAATTTTGGCCACTCACg                                                                >  2:4174379/1‑93 (MQ=255)
                          aCTCTTTCTTCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATT‑CAGAGATGAAATTTTGGCCACTCACGa                                                               <  2:2285148/93‑1 (MQ=255)
                          aCTCTTTCTTCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATT‑CAGAGATGAAATTTTGGCCACTCACGa                                                               <  2:3274623/93‑1 (MQ=255)
                          aCTCTTTCTTCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATT‑CAGAGATGAAATTTTGGCCACTCACGa                                                               <  1:4761808/93‑1 (MQ=255)
                           ctctTTCTTCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATT‑CAGAGATGAAATTTTGGCCACTCACGAg                                                              >  1:6363457/1‑93 (MQ=255)
                            tctttcttCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATT‑CAGAGATGAAATTTTGGCCACTCACGAGt                                                             <  1:3415325/93‑1 (MQ=255)
                            tctttcttCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATT‑CAGAGATGAAATTTTGGCCACTCACGAGt                                                             >  1:2064036/1‑93 (MQ=255)
                              tttcttCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATT‑CAGAGATGAAATTTTGGCCACTCACGAGTgg                                                           >  1:4404658/1‑93 (MQ=255)
                              tttcttCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATT‑CAGAGATGAAATTTTGGCCACTCACGAGTgg                                                           >  1:4219899/1‑93 (MQ=255)
                               ttcttcCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATT‑CAGAGATGAAATTTTGGCCACTCACGAGTGGc                                                          >  1:462350/1‑93 (MQ=255)
                                tcttcCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATT‑CAGAGATGAAATTTTGGCCACTCACGAGTGGcc                                                         <  2:4464725/93‑1 (MQ=255)
                                tcttcCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATT‑CAGAGATGAAATTTTGGCCACTCACGAGTGGcc                                                         >  2:2916716/1‑93 (MQ=255)
                                tcttcCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATT‑CAGAGATGAAATTTTGGCCACTCACGAGTGGcc                                                         >  2:2209977/1‑93 (MQ=255)
                                 cttcCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATT‑CAGAGATGAAATTTTGGCCACTCACGAGTGGCCt                                                        >  1:7428429/1‑93 (MQ=255)
                                  ttcCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATT‑CAGAGATGAAATTTTGGCCACTCACGAGTGGCCtt                                                       >  2:4676512/1‑93 (MQ=255)
                                   tcCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATT‑CAGAGATGAAATTTTGGCCACTCACGAGTGGCCttt                                                      <  1:134450/93‑1 (MQ=255)
                                    cccAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATT‑CAGAGATGAAATTTTGGCCACTCACGAGTGGCCtttt                                                     >  1:5339767/1‑93 (MQ=255)
                                     ccAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATT‑CAGAGATGAAATTTTGGCCACTCACGAGTGGCCttttt                                                    <  2:6961020/93‑1 (MQ=255)
                                     ccAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATT‑CAGAGATGAAATTTTGGCCACTCACGAGTGGCCttttt                                                    >  2:5643759/1‑93 (MQ=255)
                                      cAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATT‑CAGAGATGAAATTTTGGCCACTCACGAGTGGCCTttttc                                                   >  2:7223039/1‑93 (MQ=255)
                                        ggCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATT‑CAGAGATGAAATTTTGGCCACTCACGAGTGGCCTttttctt                                                 <  1:5602656/93‑1 (MQ=255)
                                        ggCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATT‑CAGAGATGAAATTTTGGCCACTCACGAGTGGCCTttttctt                                                 <  1:2837224/93‑1 (MQ=255)
                                        ggCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATT‑CAGAGATGAAATTTTGGCCACTCACGAGTGGCCTttttctt                                                 >  2:6298809/1‑93 (MQ=255)
                                        ggCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATT‑CAGAGATGAAATTTTGGCCACTCACGAGTGGCCTttttctt                                                 >  2:731726/1‑93 (MQ=255)
                                        ggCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATT‑CAGAGATGAAATTTTGGCCACTCACGAGTGGCCTttttctt                                                 >  2:5849386/1‑93 (MQ=255)
                                         gCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATT‑CAGAGATGAAATTTTGGCCACTCACGAGTGGCCTttttcttt                                                >  2:3804077/1‑93 (MQ=255)
                                           gAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATT‑CAGAGATGAAATTTTGGCCACTCACGAGTGGCCTttttcttttc                                              <  2:504757/93‑1 (MQ=255)
                                           gAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATT‑CAGAGATGAAATTTTGGCCACTCACGAGTGGCCTttttcttttc                                              >  2:5396126/1‑93 (MQ=255)
                                             gTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATT‑CAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTg                                            >  2:5381792/1‑93 (MQ=255)
                                             gTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATT‑CAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTg                                            <  1:1031867/93‑1 (MQ=255)
                                                tGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATT‑CAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCa                                         <  1:180256/93‑1 (MQ=255)
                                                tGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATT‑CAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCa                                         >  2:1007952/1‑93 (MQ=255)
                                                tGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATT‑CAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCa                                         <  1:2880084/93‑1 (MQ=255)
                                                tGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATT‑CAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCa                                         >  1:3903943/1‑93 (MQ=255)
                                                 gAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATT‑CAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAg                                        >  1:3258583/1‑93 (MQ=255)
                                                 gAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATT‑CAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAg                                        <  2:6001295/93‑1 (MQ=255)
                                                  aGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATT‑CAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAgg                                       <  2:1595901/93‑1 (MQ=255)
                                                  aGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATT‑CAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAgg                                       >  1:5916028/1‑93 (MQ=255)
                                                  aGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATT‑CAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAgg                                       <  2:4780173/93‑1 (MQ=255)
                                                  aGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATT‑CAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAgg                                       >  1:6899809/1‑93 (MQ=255)
                                                   gTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATT‑CAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGc                                      <  1:5379370/93‑1 (MQ=255)
                                                      tatGAAAGACGCGCATTTGTTATCATCATCCCTGAATT‑CAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCgtg                                   >  2:6632987/1‑93 (MQ=255)
                                                      tatGAAAGACGCGCATTTGTTATCATCATCCCTGAATT‑CAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCgtg                                   >  1:1370901/1‑93 (MQ=255)
                                                        tGAAAGACGCGCATTTGTTATCATCATCCCTGAATT‑CAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGtt                                 >  1:7052401/1‑93 (MQ=255)
                                                        tGAAAGACGCGCATTTGTTATCATCATCCCTGAATT‑CAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGtt                                 >  1:7249370/1‑93 (MQ=255)
                                                        tGAAAGACGCGCATTTGTTATCATCATCCCTGAATT‑CAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGtt                                 >  1:2469529/1‑93 (MQ=255)
                                                         gAAAGACGCGCATTTGTTATCATCATCCCTGAATT‑CAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGttt                                >  2:186822/1‑93 (MQ=255)
                                                          aaaGACGCGCATTTGTTATCATCATCCCTGAATT‑CAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGtttt                               >  2:2149707/1‑93 (MQ=255)
                                                           aaGACGCGCATTTGTTATCATCATCCCTGAATT‑CAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGttttt                              <  1:1705892/93‑1 (MQ=255)
                                                           aaGACGCGCATTTGTTATCATCATCCCTGAATT‑CAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGttttt                              <  1:6507985/93‑1 (MQ=255)
                                                            aGACGCGCATTTGTTATCATCATCCCTGAATT‑CAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGTTTTTc                             <  1:1480473/93‑1 (MQ=255)
                                                             gACGCGCATTTGTTATCATCATCCCTGAATT‑CAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGTTTTTcc                            <  1:953788/93‑1 (MQ=255)
                                                              aCGCGCATTTGTTATCATCATCCCTGAATT‑CAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGTTTTTCCa                           >  1:2540548/1‑93 (MQ=255)
                                                                gcgcATTTGTTATCATCATCCCTGAATT‑CAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGTTTTTCCAGc                         >  1:501478/1‑93 (MQ=255)
                                                                  gcATTTGTTATCATCATCCCTGAATT‑CAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGTTTTTCCAGCca                       >  1:790359/1‑93 (MQ=255)
                                                                   cATTTGTTATCATCATCCCTGAATT‑CAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGTTTTTCCAGCcac                      >  2:1281394/1‑93 (MQ=255)
                                                                   cATTTGTTATCATCATCCCTGAATT‑CAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGTTTTTCCAGCcac                      >  1:2968846/1‑93 (MQ=255)
                                                                   cATTTGTTATCATCATCCCTGAATT‑CAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGTTTTTCCAGCcac                      >  2:4943485/1‑93 (MQ=255)
                                                                    aTTTGTTATCATCATCCCTGAATT‑CAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGTTTTTCCAGCcaca                     >  1:6654138/1‑93 (MQ=255)
                                                                     tttGTTATCATCATCCCTGAATT‑CAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGTTTTTCCAGCCacac                    <  2:4040680/93‑1 (MQ=255)
                                                                     tttGTTATCATCATCCCTGAATT‑CAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGTTTTTCCAGCCacac                    <  2:3731850/93‑1 (MQ=255)
                                                                        gTTATCATCATCCCTGAATT‑CAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGTTTTTCCAGCCACACCGc                 >  2:7003243/1‑93 (MQ=255)
                                                                          tATCATCATCCCTGAATT‑CAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGTTTTTCCAGCCACACCGCaa               >  2:2738615/1‑93 (MQ=255)
                                                                           atcatcatcCCTGAATT‑CAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGTTTTTCCAGCCACACCGCaaa              <  2:6103090/93‑1 (MQ=255)
                                                                           atcatcatcCCTGAATT‑CAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGTTTTTCCAGCCACACCGCaaa              >  1:5851838/1‑93 (MQ=255)
                                                                            tcatcatcCCTGAATT‑CAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGTTTTTCCAGCCACACCGCAAAc             >  1:3928688/1‑93 (MQ=255)
                                                                             catcatcCCTGAATT‑CAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGTTTTTCCAGCCACACCGCAAACg            <  2:4683009/93‑1 (MQ=255)
                                                                             catcatcCCTGAATT‑CAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGTTTTTCCAGCCACACCGCAAACg            <  1:5397733/93‑1 (MQ=255)
                                                                                catcCCTGAATT‑CAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGTTTTTCCAGCCACACCGCAAACGGtt         >  2:5552832/1‑93 (MQ=255)
                                                                                catcCCTGAATT‑CAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGTTTTTCCAGCCACACCGCAAACGGtt         <  2:76406/93‑1 (MQ=255)
                                                                                  tcCCTGAATT‑CAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGTTTTTCCAGCCACACCGCAAACGGTTCg       <  2:6372100/93‑1 (MQ=255)
                                                                                  tcCCTGAATT‑CAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGTTTTTCCAGCCACACCGCAAACGGTTCg       >  1:4938111/1‑93 (MQ=255)
                                                                                   cccTGAATT‑CAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGTTTTTCCAGCCACACCGCAAACGGTTCgg      >  1:3694864/1‑93 (MQ=255)
                                                                                    ccTGAATT‑CAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGTTTTTCCAGCCACAACGCAAACGGTTCGGt     >  1:3764747/1‑93 (MQ=255)
                                                                                     cTGAATT‑CAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGTTTTTCCAGCCACACCGCAAACGGTTCGGTa    >  1:4804802/1‑93 (MQ=255)
                                                                                       gAATT‑CAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGTTTTTCCAGCCACACCGCAAACGGTTCGGTATc  <  1:3530580/93‑1 (MQ=255)
                                                                                       gAATT‑CAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGTTTTTCCAGCCACACCGCAAACGGTTCGGTATc  >  2:837632/1‑93 (MQ=255)
                                                                                            |                                                                                        
CGGTAATTTTTTTAAAAATTTTCTGAACTCTTTCTTCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATTCCAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGTTTTTCCAGCCACACCGCAAACGGTTCGGTATC  >  NC_000913/2812766‑2812946

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CGGTAATTTTTTTAAAAATTTTCTGAACTCTTTCTTCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATTCCAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGTTTTTCCAGCCACACCGCAAACGGTTCGGTATCAGCGGC  >  NC_000913/2812766‑2812952
                                                                                             |                                                                                             
CGGTAATTTTTTTAAAAATTTTCTGAACTCTTTCTTCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATTCA                                                                                               <  SRR4302542.7597713/94‑1 (MQ=60)
   TAATTTTTTTAAAAATTTTCTGAACTCTTTCTTCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATT‑CAGAG                                                                                           >  SRR4302542.7307619/1‑94 (MQ=60)
     ATTTTTTTAAAAATTTTCTGAACTCTTTCTTCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATT‑CAGAGAT                                                                                         <  SRR4302542.412509/94‑1 (MQ=60)
      TTTTTTTAAAAATTTTCTGAACTCTTTCTTCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATT‑CAGAGATG                                                                                        >  SRR4302542.2895933/1‑94 (MQ=60)
        TTTTTAAAAATTTTCTGAACTCTTTCTTCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATT‑CAGAGATGAA                                                                                      >  SRR4302542.5690768/1‑94 (MQ=60)
        TTTTTAAAAATTTTCTGAACTCTTTCTTCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATT‑CAGAGATGAA                                                                                      >  SRR4302542.1989094/1‑94 (MQ=60)
        TTTTTAAAAATTTTCTGAACTCTTTCTTCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAA‑TCCAGAGATGAA                                                                                      >  SRR4302542.3180881/1‑94 (MQ=60)
         TTTTAAAAATTTTCTGAACTCTTTCTTCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATT‑CAGAGATGAAA                                                                                     >  SRR4302542.3856695/1‑94 (MQ=60)
         TTTTAAAAATTTTCTGAACTCTTTCTTCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATT‑CAGAGATGAAA                                                                                     >  SRR4302542.2561432/1‑94 (MQ=60)
         TTTTAAAAATTTTCTGAACTCTTTCTTCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATT‑CAGAGATGAAA                                                                                     <  SRR4302542.747784/94‑1 (MQ=60)
           TTAAAAATTTTCTGAACTCTTTCTTCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATT‑CAGAGATGAAATT                                                                                   >  SRR4302542.1165556/1‑94 (MQ=60)
             AAAAATTTTCTGAACTCTTTCTTCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATT‑CAGAGATGAAATTTT                                                                                 >  SRR4302542.2403269/1‑94 (MQ=60)
             AAAAATTTTCTGAACTCTTTCTTCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATT‑CAGAGATGAAATTTT                                                                                 <  SRR4302542.7092319/94‑1 (MQ=60)
             AAAAATTTTCTGAACTCTTTCTTCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATT‑CAGAGATGAAATTTT                                                                                 <  SRR4302542.4769334/94‑1 (MQ=60)
              AAAATTTTCTGAACTCTTTCTTCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATT‑CAGAGATGAAATTTTG                                                                                <  SRR4302542.3233100/94‑1 (MQ=60)
              AAAATTTTCTGAACTCTTTCTTCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATT‑CAGAGATGAAATTTTG                                                                                <  SRR4302542.4221189/94‑1 (MQ=60)
              AAAATTTTCTGAACTCTTTCTTCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATT‑CAGAGATGAAATTTTG                                                                                <  SRR4302542.4360054/94‑1 (MQ=60)
              AAAATTTTCTGAACTCTTTCTTCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATT‑CAGAGATGAAATTTTG                                                                                >  SRR4302542.683285/1‑94 (MQ=60)
                AATTTTCTGAACTCTTTCTTCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATT‑CAGAGATGAAATTTTGGC                                                                              >  SRR4302542.5169558/1‑94 (MQ=60)
                 ATTTTCTGAACTCTTTCTTCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATT‑CAGAGATGAAATTTTGGCC                                                                             <  SRR4302542.683007/94‑1 (MQ=60)
                 ATTTTCTGAACTCTTTCTTCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATT‑CAGAGATGAAATTTTGGCC                                                                             >  SRR4302542.939692/1‑94 (MQ=60)
                 ATTTTCTGAACTCTTTCTTCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATT‑CAGAGATGAAATTTTGGCC                                                                             <  SRR4302542.7110256/94‑1 (MQ=60)
                 ATTTTCTGAACTCTTTCTTCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATT‑CAGAGATGAAATTTTGGCC                                                                             >  SRR4302542.6162152/1‑94 (MQ=60)
                    TTCTGAACTCTTTCTTCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATT‑CAGAGATGAAATTTTGGCCACT                                                                          <  SRR4302542.4320918/94‑1 (MQ=60)
                      CTGAACTCTTTCTTCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATT‑CAGAGATGAAATTTTGGCCACTCA                                                                        >  SRR4302542.6763190/1‑94 (MQ=60)
                      CTGAACTCTTTCTTCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATT‑CAGAGATGAAATTTTGGCCACTCA                                                                        >  SRR4302542.7420876/1‑94 (MQ=60)
                      CTGAACTCTTTCTTCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATT‑CAGAGATGAAATTTTGGCCACTCA                                                                        >  SRR4302542.2902030/1‑94 (MQ=60)
                      CTGAACTCTTTCTTCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATT‑CAGAGATGAAATTTTGGCCACTCA                                                                        >  SRR4302542.5122433/1‑94 (MQ=60)
                       TGAACTCTTTCTTCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATT‑CAGAGATGAAATTTTGGCCACTCAC                                                                       >  SRR4302542.1462724/1‑94 (MQ=60)
                       TGAACTCTTTCTTCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATT‑CAGAGATGAAATTTTGGCCACTCAC                                                                       >  SRR4302542.6350256/1‑94 (MQ=60)
                       TGAACTCTTTCTTCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATT‑CAGAGATGAAATTTTGGCCACTCAC                                                                       <  SRR4302542.1669673/94‑1 (MQ=60)
                       TGAACTCTTTCTTCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATT‑CAGAGATGAAATTTTGGCCACTCAC                                                                       <  SRR4302542.3297097/94‑1 (MQ=60)
                         AACTCTTTCTTCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATT‑CAGAGATGAAATTTTGGCCACTCACGA                                                                     >  SRR4302542.3036474/1‑94 (MQ=60)
                          ACTCTTTCTTCCGAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATT‑CAGAGATGAAATTTTGGCCACTCACGAG                                                                    <  SRR4302542.5005245/94‑1 (MQ=60)
                          ACTCTTTCTTCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATT‑CAGAGATGAAATTTTGGCCACTCACGAG                                                                    >  SRR4302542.1916231/1‑94 (MQ=60)
                          ACTCTTTCTTCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATT‑CAGAGATGAAATTTTGGCCACTCACGAG                                                                    >  SRR4302542.6426125/1‑94 (MQ=60)
                          ACTCTTTCTTCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATT‑CAGAGATGAAATTTTGGCCACTCACGAG                                                                    <  SRR4302542.4808235/94‑1 (MQ=60)
                           CTCTTTCTTCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATT‑CAGAGATGAAATTTTGGCCACTCACGAGT                                                                   <  SRR4302542.4701951/94‑1 (MQ=60)
                           CTCTTTCTTCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATT‑CAGAGATGAAATTTTGGCCACTCACGAGT                                                                   >  SRR4302542.2081878/1‑94 (MQ=60)
                           CTCTTTCTTCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATT‑CAGAGATGAAATTTTGGCCACTCACGAGT                                                                   >  SRR4302542.6698985/1‑94 (MQ=60)
                            TCTTTCTTCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATT‑CAGAGATGAAATTTTGGCCACTCACGAGTG                                                                  <  SRR4302542.2972671/94‑1 (MQ=60)
                            TCTTTCTTCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATT‑CAGAGATGAAATTTTGGCCACTCACGAGTG                                                                  >  SRR4302542.6653103/1‑94 (MQ=60)
                            TCTTTCTTCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATT‑CAGAGATGAAATTTTGGCCACTCACGAGTG                                                                  <  SRR4302542.3946214/94‑1 (MQ=60)
                            TCTTTCTTCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATT‑CAGAGATGAAATTTTGGCCACTCACGAGTG                                                                  <  SRR4302542.1083501/94‑1 (MQ=60)
                            TCTTTCTTCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATT‑CAGAGATGAAATTTTGGCCACTCACGAGTG                                                                  <  SRR4302542.3447325/94‑1 (MQ=60)
                             CTTTCTTCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATT‑CAGAGATGAAATTTTGGCCACTCACGAGTGG                                                                 >  SRR4302542.4261485/1‑94 (MQ=60)
                             CTTTCTTCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATT‑CAGAGATGAAATTTTGGCCACTCACGAGTGG                                                                 >  SRR4302542.4447915/1‑94 (MQ=60)
                             CTTTCTTCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATT‑CAGAGATGAAATTTTGGCCACTCACGAGTGG                                                                 >  SRR4302542.6998420/1‑94 (MQ=60)
                              TTTCTTCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATT‑CAGAGATGAAATTTTGGCCACTCACGAGTGGC                                                                >  SRR4302542.4982105/1‑94 (MQ=60)
                              TTTCTTCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATT‑CAGAGATGAAATTTTGGCCACTCACGAGTGGC                                                                >  SRR4302542.466353/1‑94 (MQ=60)
                               TTCTTCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATT‑CAGAGATGAAATTTTGGCCACTCACGAGTGGCC                                                               >  SRR4302542.540583/1‑94 (MQ=60)
                               TTCTTCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATT‑CAGAGATGAAATTTTGGCCACTCACGAGTGGCC                                                               <  SRR4302542.633249/94‑1 (MQ=60)
                                TCTTCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATT‑CAGAGATGAAATTTTGGCCACTCACGAGTGGCCT                                                              >  SRR4302542.2794874/1‑94 (MQ=60)
                                TCTTCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATT‑CAGAGATGAAATTTTGGCCACTCACGAGTGGCCT                                                              >  SRR4302542.7501123/1‑94 (MQ=60)
                                 CTTCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATT‑CAGAGATGAAATTTTGGCCACTCACGAGTGGCCTT                                                             >  SRR4302542.5569669/1‑94 (MQ=60)
                                 CTTCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATT‑CAGAGATGAAATTTTGGCCACTCACGAGTGGCCTT                                                             <  SRR4302542.5934709/94‑1 (MQ=60)
                                 CTTCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATT‑CAGAGATGAAATTTTGGCCACTCACGAGTGGCCTT                                                             >  SRR4302542.7000091/1‑94 (MQ=60)
                                 CTTCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATT‑CAGAGATGAAATTTTGGCCACTCACGAGTGGCCTT                                                             >  SRR4302542.1471689/1‑94 (MQ=60)
                                 CTTCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATT‑CAGAGATGAAATTTTGGCCACTCACGAGTGGCCTT                                                             >  SRR4302542.2481985/1‑94 (MQ=60)
                                   TCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATT‑CAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTT                                                           >  SRR4302542.5391645/1‑94 (MQ=60)
                                   TCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATT‑CAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTT                                                           <  SRR4302542.135536/94‑1 (MQ=60)
                                   TCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATT‑CAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTT                                                           >  SRR4302542.1261490/1‑94 (MQ=60)
                                    CCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATT‑CAGAGATGAAATTTTTGTCACTCACGAGTGGCCTTTTT                                                          >  SRR4302542.2564665/1‑94 (MQ=60)
                                    CCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATT‑CAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTT                                                          >  SRR4302542.2503997/1‑94 (MQ=60)
                                     CCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATT‑CAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTC                                                         <  SRR4302542.1794235/94‑1 (MQ=60)
                                     CCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATT‑CAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTC                                                         >  SRR4302542.3946380/1‑94 (MQ=60)
                                     CCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATT‑CAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTC                                                         >  SRR4302542.6434902/1‑94 (MQ=60)
                                      CAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCCGAATT‑CAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCT                                                        <  SRR4302542.7539376/94‑1 (MQ=60)
                                       AGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATT‑CAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTT                                                       >  SRR4302542.6319656/1‑94 (MQ=60)
                                        GGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATT‑CAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTT                                                      <  SRR4302542.5657085/94‑1 (MQ=60)
                                        GGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATT‑CAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTT                                                      <  SRR4302542.2864493/94‑1 (MQ=60)
                                         GCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATT‑CAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTT                                                     <  SRR4302542.6394796/94‑1 (MQ=60)
                                          CGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATT‑CAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTC                                                    >  SRR4302542.4823951/1‑94 (MQ=60)
                                           GAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATT‑CAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCT                                                   <  SRR4302542.5415371/94‑1 (MQ=60)
                                           GAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATT‑CAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCT                                                   <  SRR4302542.5454967/94‑1 (MQ=60)
                                            AGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATT‑CAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTG                                                  >  SRR4302542.5328452/1‑94 (MQ=60)
                                             GTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATT‑CAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGT                                                 <  SRR4302542.1040748/94‑1 (MQ=60)
                                               CTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATT‑CAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCA                                               >  SRR4302542.3942483/1‑94 (MQ=60)
                                               CTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATT‑CAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCA                                               >  SRR4302542.4525767/1‑94 (MQ=60)
                                               CTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATT‑CAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCA                                               >  SRR4302542.4414444/1‑94 (MQ=60)
                                                TGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATT‑CAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAG                                              >  SRR4302542.3289360/1‑94 (MQ=60)
                                                TGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATT‑CAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAG                                              <  SRR4302542.181785/94‑1 (MQ=60)
                                                TGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATT‑CAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAG                                              <  SRR4302542.2907748/94‑1 (MQ=60)
                                                 GAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATT‑CAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGG                                             <  SRR4302542.6047805/94‑1 (MQ=60)
                                                 GAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATT‑CAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGG                                             >  SRR4302542.5973359/1‑94 (MQ=60)
                                                 GAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATT‑CAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGG                                             >  SRR4302542.5973358/1‑94 (MQ=60)
                                                 GAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATT‑CAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGG                                             <  SRR4302542.3929655/94‑1 (MQ=60)
                                                 GAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATT‑CAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGG                                             >  SRR4302542.6967559/1‑94 (MQ=60)
                                                 GAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATT‑CAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGG                                             <  SRR4302542.4573249/94‑1 (MQ=60)
                                                 GAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATT‑CAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGG                                             >  SRR4302542.4271875/1‑94 (MQ=60)
                                                   GTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATT‑CAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCG                                           <  SRR4302542.1533548/94‑1 (MQ=60)
                                                   GTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATT‑CAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCG                                           <  SRR4302542.5431632/94‑1 (MQ=60)
                                                     ATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATT‑CAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTG                                         >  SRR4302542.1382754/1‑94 (MQ=60)
                                                     ATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATT‑CAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTG                                         >  SRR4302542.5050465/1‑94 (MQ=60)
                                                       ATGAAAGACGCGCATTTGTTATCATCATCCCTGAATT‑CAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGTT                                       >  SRR4302542.7121636/1‑94 (MQ=60)
                                                       ATGAAAGACGCGCATTTGTTATCATCATCCCTGAATT‑CAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGTT                                       >  SRR4302542.7320450/1‑94 (MQ=60)
                                                       ATGAAAGACGCGCATTTGTTATCATCATCCCTGAATT‑CAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGTT                                       >  SRR4302542.2493500/1‑94 (MQ=60)
                                                        TGAAAGACGCGCATTTGTTATCATCATCCCTGAATT‑CAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGTTT                                      <  SRR4302542.1365048/94‑1 (MQ=60)
                                                        TGAAAGACGCGCATTTGTTATCATCATCCCTGAATT‑CAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGTTT                                      <  SRR4302542.7297531/94‑1 (MQ=60)
                                                         GAAAGACGCGCATTTGTTATCATCATCCCTGAATT‑CAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGTTTT                                     <  SRR4302542.1315788/94‑1 (MQ=60)
                                                           AAGACGCGCATTTGTTATCATCATCCCTGAATT‑CAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGTTTTTC                                   <  SRR4302542.1720678/94‑1 (MQ=60)
                                                           AAGACGCGCATTTGTTATCATCATCCCTGAATT‑CAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGTTTTTC                                   <  SRR4302542.6572132/94‑1 (MQ=60)
                                                           AAGACGCGCATTTGTTATCATCATCCCTGAATT‑CAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGCGTTTTTC                                   >  SRR4302542.2626062/1‑94 (MQ=60)
                                                            AGACGCGCATTTGTTATCATCATCCCTGAATT‑CAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGTTTTTCC                                  <  SRR4302542.136792/94‑1 (MQ=60)
                                                            AGACGCGCATTTGTTATCATCATCCCTGAATT‑CAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGTTTTTCC                                  <  SRR4302542.1493251/94‑1 (MQ=60)
                                                             GACGCGCATTTGTTATCATCATCCCTGAATT‑CAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGTTTTTCCA                                 <  SRR4302542.961976/94‑1 (MQ=60)
                                                             GACGCGCATTTGTTATCATCATCCCTGAATT‑CAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGTTTTTCCA                                 >  SRR4302542.2565177/1‑94 (MQ=60)
                                                               CGCGCATTTGTTATCATCATCCCTGAATT‑CAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGTTTTTCCAGC                               >  SRR4302542.505828/1‑94 (MQ=60)
                                                                 CGCATTTGTTATCATCATCCCTGAATT‑CAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGTTTTTCCAGCCA                             >  SRR4302542.797169/1‑94 (MQ=60)
                                                                 CGCATTTGTTATCATCATCCCTGAATT‑CAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGTTTTTCCAGCCA                             >  SRR4302542.5112958/1‑94 (MQ=60)
                                                                  GCATTTGTTATCATCATCCCTGAATT‑CAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGTTTTTCCAGCCAC                            >  SRR4302542.2997288/1‑94 (MQ=60)
                                                                  GCATTTGTTATCATCATCCCTGAATT‑CAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGTTTTTCCAGCCAC                            <  SRR4302542.6777024/94‑1 (MQ=60)
                                                                   CATTTGTTATCATCATCCCTGAATT‑CAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGTTTTTCCAGCCACA                           >  SRR4302542.6480568/1‑94 (MQ=60)
                                                                   CATTTGTTATCATCATCCCTGAATT‑CAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGTTTTTCCAGCCACA                           >  SRR4302542.6719701/1‑94 (MQ=60)
                                                                    ATTTGTTATCATCATCCCTGAATT‑CAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGTTTTTCCAGCCACAC                          >  SRR4302542.4975591/1‑94 (MQ=60)
                                                                     TTTGTTATCATCATCCCTGAATT‑CAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGTTTTTCCAGCCACACC                         <  SRR4302542.3475185/94‑1 (MQ=60)
                                                                        GTTATCATCATCCCTGAATT‑CAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGTTTTTCCAGCCACACCGCA                      <  SRR4302542.3185017/94‑1 (MQ=60)
                                                                        GTTATCATCATCCCTGAATT‑CAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGTTTTTCCAGCCACACCGCA                      <  SRR4302542.4410763/94‑1 (MQ=60)
                                                                        GTTATCATCATCCCTGAATT‑CAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGTTTTTCCAGCCACACCGCA                      >  SRR4302542.3747456/1‑94 (MQ=60)
                                                                         TTATCATCATCCCTGAATT‑CAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGTTTTTCCAGCCACACCGCAA                     <  SRR4302542.1002999/94‑1 (MQ=60)
                                                                         TTATCATCATCCCTGAATT‑CAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGTTTTTCCAGCCACACCGCAA                     >  SRR4302542.3636316/1‑94 (MQ=60)
                                                                          TATCATCATCCCTGAATT‑CAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGTTTTTCCAGCCACACCGCAAA                    >  SRR4302542.5908588/1‑94 (MQ=60)
                                                                           ATCATCATCCCTGAATT‑CAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGTTTTTCCAGCCACACCGCAAAC                   >  SRR4302542.3967451/1‑94 (MQ=60)
                                                                           ATCATCATCCCTGAATT‑CAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGTTTTTCCAGCCACACCGCAAAC                   <  SRR4302542.5431962/94‑1 (MQ=60)
                                                                             CATCATCCCTGAATT‑CAGAGATGAAATTTTGGCCACTCACGGGTGGCCTTTTTCTTTTCTGTCAGGCGTGTTTTTCCAGCCACACCGCAAACGG                 <  SRR4302542.2446020/94‑1 (MQ=60)
                                                                             CATCATCCCTGAATT‑CAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGTTTTTCCAGCCACACCGCAAACGG                 <  SRR4302542.5450159/94‑1 (MQ=60)
                                                                               TCATCCCTGAATT‑CAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGTTTTTCCAGCCACACCGCAAACGGTT               >  SRR4302542.3585870/1‑94 (MQ=60)
                                                                                CATCCCTGAATT‑CAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGTTTTTCCAGCCACACCGCAAACGGTTC              <  SRR4302542.3519023/94‑1 (MQ=60)
                                                                                 ATCCCTGAATT‑CAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGTTTTTCCAGCCACACCGCAAACGGTTCG             >  SRR4302542.4986106/1‑94 (MQ=60)
                                                                                 ATCCCTGAATT‑CAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGTTTTTCCAGCCACACCGCAAACGGTTCG             >  SRR4302542.836020/1‑94 (MQ=60)
                                                                                 ATCCCTGAATT‑CAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGTTTTTCCAGCCACACCGCAAACGGTTCG             >  SRR4302542.6074626/1‑94 (MQ=60)
                                                                                  TCCCTGAATT‑CAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGTTTTTCCAGCCACACCGCAAACGGTTCGG            >  SRR4302542.3731426/1‑94 (MQ=60)
                                                                                  TCCCTGAATT‑CAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGTTTTTCCAGCCACACCGCAAACGGTTCGG            >  SRR4302542.5256413/1‑94 (MQ=60)
                                                                                  CCCCTGAATT‑CAGAGATGAAATTTTGGCCCCTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGTTTTTCCAGCCACACCGCAAACGGTTCGG            <  SRR4302542.4048710/94‑1 (MQ=60)
                                                                                   CCCTGAATT‑CAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGTTTTTCCAGCCACACCGCAAACGGTTCGGT           >  SRR4302542.220334/1‑94 (MQ=60)
                                                                                   CCCTGAATT‑CAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGTTTTTCCAGCCACAACGCAAACGGTTCGGT           >  SRR4302542.3801982/1‑94 (MQ=60)
                                                                                   CCCTGAATT‑CAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGCGTTTTTCCAGCCACACCGCAAACGGTTCGGT           >  SRR4302542.2528152/1‑94 (MQ=60)
                                                                                    CCTGAATT‑CAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGTTTTTCCAGCCACACCGCAAACGGTTCGGTA          >  SRR4302542.4851612/1‑94 (MQ=60)
                                                                                    CCTGAATT‑CAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGTTTTTCCAGCCACACCGCAAACGGTTCGGTA          >  SRR4302542.4191115/1‑94 (MQ=60)
                                                                                    CCTGAATT‑CAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGTTTTTCCAGCCACACCGCAAACGGTTCGGTA          <  SRR4302542.2280056/94‑1 (MQ=60)
                                                                                     CTGAATT‑CAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGTTTTTCCAGCCACACCGCAAACGGTTCGGTta         >  SRR4302542.3744557/1‑92 (MQ=60)
                                                                                     CTGAATT‑CAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGTTTTTCCAGCCACACCGCAAACGGTTCGGTAT         >  SRR4302542.2988468/1‑94 (MQ=60)
                                                                                      TGAATT‑CAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGTTTTTCCAGCCACACCGCAAACGGTTCGGTATC        >  SRR4302542.3177928/1‑94 (MQ=60)
                                                                                      TGAATT‑CAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGTTTTTCCAGCCACACCGCAAACGGTTCGGTATC        >  SRR4302542.1730559/1‑94 (MQ=60)
                                                                                       GAATT‑CAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGTTTTTCCAGCCACACCGCAAACGGTTCGGTATCA       <  SRR4302542.3563583/94‑1 (MQ=60)
                                                                                             CAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGTTTTTCCAGCCACACCGCAAACGGTTCGGTATCAGCGGC  >  SRR4302542.5887791/1‑94 (MQ=60)
                                                                                             |                                                                                             
CGGTAATTTTTTTAAAAATTTTCTGAACTCTTTCTTCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATTCCAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGTTTTTCCAGCCACACCGCAAACGGTTCGGTATCAGCGGC  >  NC_000913/2812766‑2812952

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑