Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1061 294.1 15065038 98.6% 14854127 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,823,571 A→G L10P (CTT→CCT)  mltB ← membrane‑bound lytic murein transglycosylase B

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,823,5710AG82.5% 527.5 / 112.5 251L10P (CTT→CCT) mltBmembrane‑bound lytic murein transglycosylase B
Reads supporting (aligned to +/- strand):  ref base A (23/21);  new base G (95/112);  total (118/133)
Fisher's exact test for biased strand distribution p-value = 5.07e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.66e-01

Only 100 of 257 total aligned reads displayed.

GCAGGAAGCCGCCAGACGGCGTTCCGGTGGTCGTATCAGTCTCAGTAGGTTTTGGCTTGCTGCTACAGGCGGCAAGCAACACAAAAAGGGGAAGCAATGTTACATAACGACGCTTGAACATGAGGGGTCCATTTAACAGATTCAACCAGGGGCAAGTATGGTAAAGCATCACGCCCCGCACAAGG  >  NC_000913/2823479‑2823663
                                                                                            |                                                                                            
gCAGGAAGCCGCCAGACGGCGTTCCGGTGGTCGTATCAGTCTCAGTAGGTTTTGGCTTGCTGCTACAGGCGGCAAGCAACACAAAAAGGGGaa                                                                                              <  2:5694784/93‑1 (MQ=255)
gCAGGAAGCCGCCAGACGGCGTTCCGGTGGTCGTATCAGTCTCAGTAGGTTTTGGCTTGCTGCTACAGGCGGCAAGCAACACAAAAAGGGGaa                                                                                              <  2:5441594/93‑1 (MQ=255)
 cAGGAAGCCGCCAGACGGCGTTCCGGTGGTCGTATCAGTCTCAGTAGGTTTTGGCTTGCTGCTACAGGCGGCAAGCAACACAAAAAGGGGAAg                                                                                             >  1:7207896/1‑93 (MQ=255)
 cAGGAAGCCGCCAGACGGCGTTCCGGTGGTCGTATCAGTCTCAGTAGGTTTTGGCTTGCTGCTACAGGCGGCAAGCAACACAAAAAGGGGAAg                                                                                             >  1:812440/1‑93 (MQ=255)
  gggAAGCCGCCAGACGGCGTTCCGGTGGTCGTATCACTCTCAGTATGTTTTGGCTTGCTGCTACAGGCGGCAAGCAACACAAAAAGGGGAGGc                                                                                            <  1:3592353/92‑1 (MQ=255)
  aGGAAGCCGCCAGACGGCGTTCCGGTGGTCGTATCAGTCTCAGTAGGTTTTGGCTTGCTGCTACAGGCGGCAAGCAACACAAAAAGGGGAGGc                                                                                            <  2:2470113/93‑1 (MQ=255)
  aGGAAGCCGCCAGACGGCGTTCCGGTGGTCGTATCAGTCTCAGTAGGTTTTGGCTTGCTGCTACAGGCGGCAAGCAACACAAAAAGGGGAGGc                                                                                            >  2:1511957/1‑93 (MQ=255)
   ggAAGCCGCCAGACGGCGTTCCGGTGGTCGTATCAGTCTCAGTAGGTTTTGGCTTGCTGCTACAGGCGGCAAGCAACACAAAAAGGGGAGGCa                                                                                           >  1:6661499/1‑93 (MQ=255)
   ggAAGCCGCCAGACGGCGTTCCGGTGGTCGTATCAGTCTCAGTAGGTTTTGGCTTGCTGCTACAGGCGGCAAGCAACACAAAAAGGGGAGGCa                                                                                           >  1:3136302/1‑93 (MQ=255)
   ggAAGCCGCCAGACGGCGTTCCGGTGGTCGTATCAGTCTCAGTAGGTTTTGGCTTGCTGCTACAGGCGGCAAGCAACACAAAAAGGGGAGGCa                                                                                           >  2:3085564/1‑93 (MQ=255)
   ggAAGCCGCCAGACGGCGTTCCGGTGGTCGTATCAGTCTCAGTAGGTTTTGGCTTGCTGCTACAGGCGGCAAGCAACACAAAAAGGGGAGGCa                                                                                           >  2:3243638/1‑93 (MQ=255)
   ggAAGCCGCCAGACGGCGTTCCGGTGGTCGTATCAGTCTCAGTAGGTTTTGGCTTGCTGCTACAGGCGGCAAGCAACACAAAAAGGGGAGGCa                                                                                           >  2:62739/1‑93 (MQ=255)
    gAAGCCGCCAGACGGCGTTCCGGTGGTCGTATCAGTCTCAGTAGGTTTTGGCTTGCTGCTACAGGCGGCAAGCAACACAAAAAGGGGAGGCaa                                                                                          >  2:2146893/1‑93 (MQ=255)
      aGCCGCCAGACGGCGTTCCGGTGGTCGTATCAGTCTCAGTAGGTTTTGGCTTGCTGCTACAGGCGGCAAGCAACACAAAAAGGGGAAGCAATg                                                                                        >  1:4239300/1‑93 (MQ=255)
       gccgccAGACGGCGTTCCGGTGGTCGTATCAGTCTCAGTAGGTTTTGGCTTGCTGCTACAGGCGGCAAGCAACACAAAAAGGGGAGGCAATGt                                                                                       >  2:537049/1‑93 (MQ=255)
       gccgccAGACGGCGTTCCGGTGGTCGTATCAGTCTCAGTAGGTTTTGGCTTGCTGCTACAGGCGGCAAGCAACACAAAAAGGGGAAGCAATGt                                                                                       >  2:1111885/1‑93 (MQ=255)
         cgccAGACGGCGTTCCGGTGGTCGTATCAGTCTCAGTAGGTTTTGGCTTGCTGCTACAGGCGGCAAGCAACACAAAAAGGGGAGGCAATGTTa                                                                                     >  1:5209063/1‑93 (MQ=255)
          gccAGACGGCGTTCCGGTGGTCGTATCAGTCTCAGTAGGTTTTGGCTTGCTGCTACAGGCGGCAAGCAACACAAAAAGGGGAGGCAATGTTAc                                                                                    <  1:4609395/93‑1 (MQ=255)
          gccAGACGGCGTTCCGGTGGTCGTATCAGTCTCAGTAGGTTTTGGCTTGCTGCTACAGGCGGCAAGCAACACAAAAAGGGGAGGCAATGTTAc                                                                                    <  1:4866329/93‑1 (MQ=255)
          gccAGACGGCGTTCCGGTGGTCGTATCAGTCTCAGTAGGTTTTGGCTTGCTGCTACAGGCGGCAAGCAACACAAAAAGGGGAAGCAATGTTAc                                                                                    <  2:6259694/93‑1 (MQ=255)
           ccAGACGGCGTTCCGGTGGTCGTATCAGTCTCAGTAGGTTTTGGCTTGCTGCTACAGGCGGCAAGCAACACAAAAAGGGGAGGCAATGTTACa                                                                                   <  2:3482158/93‑1 (MQ=255)
           ccAGACGGCGTTCCGGTGGTCGTATCAGTCTCAGTAGGTTTTGGCTTGCTGCTACAGGCGGCAAGCAACACAAAAAGGGGAGGCAATGTTACa                                                                                   <  1:1576465/93‑1 (MQ=255)
             aGACGGCGTTCCGGTGGTCGTATCAGTCTCAGTAGGTTTTGGCTTGCTGCTACAGGCGGCAAGCAACACAAAAAGGGGAGGCAATGTTACATa                                                                                 <  2:4688127/93‑1 (MQ=255)
             aGACGGCGTTCCGGTGGTCGTATCAGTCTCAGTAGGTTTTGGCTTGCTGCTACAGGCGGCAAGCAACACAAAAAGGGGAAGCAATGTTACATa                                                                                 >  1:6539081/1‑93 (MQ=255)
              gACGGCGTTCCGGTGGTCGTATCAGTCTCAGTAGGTTTTGGCTTGCTGCTACAGGCGGCAAGCAACACAAAAAGGGGAGGCAATGTTACATaa                                                                                >  2:7050785/1‑93 (MQ=255)
              gACGGCGTTCCGGTGGTCGTATCAGTCTCAGTAGGTTTTGGCTTGCTGCTACAGGCGGCAAGCAACACAAAAAGGGGAAGCAATGTTACATaa                                                                                <  1:1124867/93‑1 (MQ=255)
               aCGGCGTTCCGGTGGTCGTATCAGTCTCAGTAGGTTTTGGCTTGCTGCTACAGGCGGCAAGCAACACAAAAAGGGGAAGCAATGTTACATAac                                                                               <  1:6698940/93‑1 (MQ=255)
                 ggCGTTCCGGTGGTCGTATCAGTCTCAGTAGGTTTTGGCTTGCTGCTACAGGCGGCAAGCAACACAAAAAGGGGAGGCAATGTTACATAacga                                                                             <  2:4608240/93‑1 (MQ=255)
                 ggCGTTCCGGTGGTCGTATCAGTCTCAGTAGGTTTTGGCTTGCTGCTACAGGCGGCAAGCAACACAAAAAGGGGAGGCAATGTTACATAacga                                                                             >  1:6741281/1‑93 (MQ=255)
                 ggCGTTCCGGTGGTCGTATCAGTCTCAGTAGGTTTTGGCTTGCTGCTACAGGCGGCAAGCAACACAAAAAGGGGAGGCAATGTTACATAacga                                                                             >  1:4711303/1‑93 (MQ=255)
                  gCGTTCCGGTGGTCGTATCAGTCTCAGTAGGTTTTGGCTTGCTGCTACAGGCGGCAAGCAACACAAAAAGGGGAGGCAATGTTACATAacgac                                                                            >  2:5225174/1‑93 (MQ=255)
                    gTTCCGGTGGTCGTATCAGTCTCAGTAGGTTTTGGCTTGCTGCTACAGGCGGCAAGCAACACAAAAAGGGGAGGCAATGTTACATAACGACGc                                                                          >  1:2776336/1‑93 (MQ=255)
                     ttCCGGTGGTCGTATCAGTCTCAGTAGGTTTTGGCTTGCTGCTACAGGCGGCAAGCAACACAAAAAGGGGAGGCAATGTTACATAACGACGCt                                                                         >  1:756746/1‑93 (MQ=255)
                      tCCGGTGGTCGTATCAGTCTCAGTAGGTTTTGGCTTGCTGCTACAGGCGGCAAGCAACACAAAAAGGGGAGGCAATGTTACATAACGACGCtt                                                                        >  2:3406837/1‑93 (MQ=255)
                      tCCGGTGGTCGTATCAGTCTCAGTAGGTTTTGGCTTGCTGCTACAGGCGGCAAGCAACACAAAAAGGGGAAGCAATGTTACATAACGACGCtt                                                                        >  1:3829638/1‑93 (MQ=255)
                       ccGGTGGTCGTATCAGTCTCAGTAGGTTTTGGCTTGCTGCTACAGGCGGCAAGCAACACAAAAAGGGGAGGCAATGTTACATAACGACGCTTg                                                                       <  1:4005387/93‑1 (MQ=255)
                         ggtggtCGTATCAGTCTCAGTAGGTTTTGGCTTGCTGCTACAGGCGGCAAGCAACACAAAAAGGGGAGGCAATGTTACATAACGACGCTTGaa                                                                     <  1:5612709/93‑1 (MQ=255)
                          gtggtCGTATCAGTCTCAGTAGGTTTTGGCTTGCTGCTACAGGCGGCAAGCAACACAAAAAGGGGAGGCAATGTTACATAACGACGCTTGAAc                                                                    >  2:6084422/1‑93 (MQ=255)
                          gtggtCGTATCAGTCTCAGTAGGTTTTGGCTTGCTGCTACAGGCGGCAAGCAACACAAAAAGGGGAGGCAATGTTACATAACGACGCTTGAAc                                                                    >  2:1703492/1‑93 (MQ=255)
                           tggtCGTATCAGTCTCAGTAGGTTTTGGCTTGCTGCTACAGGCGGCAAGCAACACAAAAAGGGGAGGCAATGTTACATAACGACGCTTGAACa                                                                   <  1:1225539/93‑1 (MQ=255)
                           tggtCGTATCAGTCTCAGTAGGTTTTGGCTTGCTGCTACAGGCGGCAAGCAACACAAAAAGGGGAGGCAATGTTACATAACGACGCTTGAACa                                                                   <  2:3719742/93‑1 (MQ=255)
                           tggtCGTATCAGTCTCAGTAGGTTTTGGCTTGCTGCTACAGGCGGCAAGCAACACAAAAAGGGGAGGCAATGTTACATAACGACGCTTGAACa                                                                   <  2:3506738/93‑1 (MQ=255)
                           tggtCGTATCAGTCTCAGTAGGTTTTGGCTTGCTGCTACAGGCGGCAAGCAACACAAAAAGGGGAAGCAATGTTACATAACGACGCTTGAACa                                                                   <  1:7368944/93‑1 (MQ=255)
                             gtCGTATCAGTCTCAGTAGGTTTTGGCTTGCTGCTACAGGCGGCAAGCAACACAAAAAGGGGAGGCAATGTTACATAACGACGCTTGAACATg                                                                 <  1:5061520/93‑1 (MQ=255)
                             gtCGTATCAGTCTCAGTAGGTTTTGGCTTGCTGCTACAGGCGGCAAGCAACACAAAAAGGGGAGGCAATGTTACATAACGACGCTTGAACATg                                                                 >  2:6630436/1‑93 (MQ=255)
                             gtCGTATCAGTCTCAGTAGGTTTTGGCTTGCTGCTACAGGCGGCAAGCAACACAAAAAGGGGAGGCAATGTTACATAACGACGCTTGAACATg                                                                 <  2:4070622/93‑1 (MQ=255)
                              tCGTATCAGTCTCAGTAGGTTTTGGCTTGCTGCTACAGGCGGCAAGCAACACAAAAAGGGGAGGCAATGTTACATAACGACGCTTGAACATGa                                                                >  2:291242/1‑93 (MQ=255)
                                gTATCAGTCTCAGTAGGTTTTGGCTTGCTGCTACAGGCGGCAAGCAACACAAAAAGGGGAGGCAATGTTACATAACGACGCTTGAACATGAgg                                                              >  1:6169683/1‑93 (MQ=255)
                                gTATCAGTCTCAGTAGGTTTTGGCTTGCTGCTACAGGCGGCAAGCAACACAAAAAGGGGAGGCAATGTTACATAACGACGCTTGAACATGAgg                                                              >  1:4822060/1‑93 (MQ=255)
                                gTATCAGTCTCAGTAGGTTTTGGCTTGCTGCTACAGGCGGCAAGCAACACAAAAAGGGGAAGCAATGTTACATAACGACGCTTGAACATGAgg                                                              <  1:1807221/93‑1 (MQ=255)
                                  aTCAGTCTCAGTAGGTTTTGGCTTGCTGCTACAGGCGGCAAGCAACACAAAAAGGGGAAGCAATGTTACATAACGACGCTTGAACATGAgggg                                                            >  2:4212829/1‑93 (MQ=255)
                                   tCAGTCTCAGTAGGTTTTGGCTTGCTGCTACAGGCGGCAAGCAACACAAAAAGGGGAAGCAATGTTACATAACGACGCTTGAACATGAGGGGt                                                           <  1:5629015/93‑1 (MQ=255)
                                    cAGTCTCAGTAGGTTTTGGCTTGCTGCTACAGGCGGCAAGCAACACAAAAAGGGGAGGCAATGTTACATAACGACGCTTGAACATGAGGGGTc                                                          <  1:932729/93‑1 (MQ=255)
                                      gTCTCAGTAGGTTTTGGCTTGCTGCTACAGGCGGCAAGCAACACAAAAAGGGGAGGCAATGTTACATAACGACGCTTGAACATGAGGGGTCCa                                                        >  1:4335815/1‑93 (MQ=255)
                                        ctcAGTAGGTTTTGGCTTGCTGCTACAGGCGGCAAGCAACACAAAAAGGGGAGGCAATGTTACATAACGACGCTTGAACATGAGGGGTCCAtt                                                      <  2:4802021/93‑1 (MQ=255)
                                         tcAGTAGGTTTTGGCTTGCTGCTACAGGCGGCAAGCAACACAAAAAGGGGAGGCAATGTTACATAACGACGCTTGAACATGAGGGGTCCAttt                                                     >  1:5520445/1‑93 (MQ=255)
                                           aGTAGGTTTTGGCTTGCTGCTACAGGCGGCAAGCAACACAAAAAGGGGAGGCAATGTTACATAACGACGCTTGAACATGAGGGGTCCATTTaa                                                   >  2:3618856/1‑93 (MQ=255)
                                            gTAGGTTTTGGCTTGCTGCTACAGGCGGCAAGCAACACAAAAAGGGGAGGCAATGTTACATAACGACGCTTGAACATGAGGGGTCCATTTAAc                                                  >  1:1907375/1‑93 (MQ=255)
                                            gTAGGTTTTGGCTTGCTGCTACAGGCGGCAAGCAACACAAAAAGGGGAGGCAATGTTACATAACGACGCTTGAACATGAGGGGTCCATTTAAc                                                  <  1:5873810/93‑1 (MQ=255)
                                             tAGGTTTTGGCTTGCTGCTACAGGCGGCAAGCAACACAAAAAGGGGAGGCAATGTTACATAACGACGCTTGAACATGAGGGGTCCATTTAACa                                                 >  2:2616198/1‑93 (MQ=255)
                                              aGGTTTTGGCTTGCTGCTACAGGCGGCAAGCAACACAAAAAGGGGAGGCAATGTTACATAACGACGCTTGAACATGAGGGGTCCATTTAACAg                                                <  1:2689503/93‑1 (MQ=255)
                                              aGGTTTTGGCTTGCTGCTACAGGCGGCAAGCAACACAAAAAGGGGAGGCAATGTTACATAACGACGCTTGAACATGAGGGGTCCATTTAACAg                                                >  2:491115/1‑93 (MQ=255)
                                              aGGTTTTGGCTTGCTGCTACAGGCGGCAAGCAACACAAAAAGGGGAAGCAATGTTACATAACGACGCTTGAACATGAGGGGTCCATTTAACAg                                                >  2:7222501/1‑93 (MQ=255)
                                               ggTTTTGGCTTGCTGCTACAGGCGGCAAGCAACACAAAAAGGGGAGGCAATGTTACATAACGACGCTTGAACATGAGGGGTCCATTTAACAGa                                               >  1:6363198/1‑93 (MQ=255)
                                               ggTTTTGGCTTGCTGCTACAGGCGGCAAGCAACACAAAAAGGGGAGGCAATGTTACATAACGACGCTTGAACATGAGGGGTCCATTTAACAGa                                               <  2:6941515/93‑1 (MQ=255)
                                               ggTTTTGGCTTGCTGCTACAGGCGGCAAGCAACACAAAAAGGGGAGGCAATGTTACATAACGACGCTTGAACATGAGGGGTCCATTTAACAGa                                               <  2:5585392/93‑1 (MQ=255)
                                                gTTTTGGCTTGCTGCTACAGGCGGCAAGCAACACAAAAAGGGGAGGCAATGTTACATAACGACGCTTGAACATGAGGGGTCCATTTAACAGAt                                              <  1:2367204/93‑1 (MQ=255)
                                                gTTTTGGCTTGCTGCTACAGGCGGCAAGCAACACAAAAAGGGGAAGCAATGTTACATAACGACGCTTGAACATGAGGGGTCCATTTAACAGAt                                              <  2:4953446/93‑1 (MQ=255)
                                                 ttttGGCTTGCTGCTACAGGCGGCAAGCAACACAAAAAGGGGAGGCAATGTTACATAACGACGCTTGAACATGAGGGGTCCATTTAACAGAtt                                             <  1:4448073/93‑1 (MQ=255)
                                                  tttGGCTTGCTGCTACAGGCGGCAAGCAACACAAAAAGGGGAGGCAATGTTACATAACGACGCTTGAACATGAGGGGTCCATTTAACAGATTc                                            <  1:3216101/93‑1 (MQ=255)
                                                  tttGGCTTGCTGCTACAGGCGGCAAGCAACACAAAAAGGGGAGGCAATGTTACATAACGACGCTTGAACATGAGGGGTCCATTTAACAGATTc                                            <  2:815500/93‑1 (MQ=255)
                                                     ggCTTGCTGCTACAGGCGGCAAGCAACACAAAAAGGGGAGGCAATGTTACATAACGACGCTTGAACATGAGGGGTCCATTTAACAGATTCAAc                                         <  1:2405010/93‑1 (MQ=255)
                                                      gCTTGCTGCTACAGGCGGCAAGCAACACAAAAAGGGGAGGCAATGTTACATAACGACGCTTGAACATGAGGGGTCCATTTAACAGATTCAAcc                                        <  2:7151314/93‑1 (MQ=255)
                                                       cTTGCTGCTACAGGCGGCAAGCAACACAAAAAGGGGAGGCAATGTTACATAACGACGCTTGAACATGAGGGGTCCATTTAACAGATTCAACCa                                       <  1:3666810/93‑1 (MQ=255)
                                                        ttGCTGCTACAGGCGGCAAGCAACACAAAAAGGGGAGGCAATGTTACATAACGACGCTTGAACATGAGGGGTCCATTTAACAGATTCAACCAg                                      <  1:131756/93‑1 (MQ=255)
                                                        ttGCTGCTACAGGCGGCAAGCAACACAAAAAGGGGAGGCAATGTTACATAACGACGCTTGAACATGAGGGGTCCATTTAACAGATTCAACCAg                                      <  2:6439780/93‑1 (MQ=255)
                                                        ttGCTGCTACAGGCGGCAAGCAACACAAAAAGGGGAGGCAATGTTACATAACGACGCTTGAACATGAGGGGTCCATTTAACAGATTCAACCAg                                      <  2:5857079/93‑1 (MQ=255)
                                                         tgctgcTACAGGCGGCAAGCAACACAAAAAGGGGAAGCAATGTTACATAACGACGCTTGAACATGAGGGGTCCATTTAACAGATTCAACCAgg                                     <  1:1664910/93‑1 (MQ=255)
                                                              ctACAGGCGGCAAGCAACACAAAAAGGGGAGGCAATGTTACATAACGACGCTTGAACATGAGGGGTCCATTTAACAGATTCAACCAGGGGCaa                                <  2:377449/93‑1 (MQ=255)
                                                                aCAGGCGGCAAGCAACACAAAAAGGGGAGGCAATGTTACATAACGACGCTTGAACATGAGGGGTCCATTTAACAGATTCAACCAGGGGCAAGt                              <  1:4519443/93‑1 (MQ=255)
                                                                 cAGGCGGCAAGCAACACAAAAAGGGGAGGCAATGTTACATAACGACGCTTGAACATGAGGGGTCCATTTAACAGATTCAACCAGGGGCAAGTa                             >  2:5988308/1‑93 (MQ=255)
                                                                 cAGGCGGCAAGCAACACAAAAAGGGGAGGCAATGTTACATAACGACGCTTGAACATGAGGGGTCCATTTAACAGATTCAACCAGGGGCAAGTa                             <  2:4293722/93‑1 (MQ=255)
                                                                  aGGCGGCAAGCAACACAAAAAGGGGAGGCAATGTTACATAACGACGCTTGAACATGAGGGGTCCATTTAACAGATTCAACCAGGGGCAAGTAt                            >  1:3498897/1‑93 (MQ=255)
                                                                    gcggcAAGCAACACAAAAAGGGGAGGCAATGTTACATAACGACGCTTGAACATGAGGGGTCCATTTAACAGATTCAACCAGGGGCAAGTATgg                          >  1:3088590/1‑93 (MQ=255)
                                                                      ggcAAGCAACACAAAAAGGGGAAGCAATGTTACATAACGACGCTTGAACATGAGGGGTCCATTTAACAGATTCAACCAGGGGCAAGTATGGTa                        <  1:6863562/93‑1 (MQ=255)
                                                                        caagcaaCACAAAAAGGGGAGGCAATGTTACATAACGACGCTTGAACATGAGGGGTCCATTTAACAGATTCAACCAGGGGCAAGTATGGTaaa                      >  2:4104437/1‑93 (MQ=255)
                                                                          agcaaCACAAAAAGGGGAGGCAATGTTACATAACGACGCTTGAACATGAGGGGTCCATTTAACAGATTCAACCAGGGGCAAGTATGGTAAAGc                    <  1:2603475/93‑1 (MQ=255)
                                                                          agcaaCACAAAAAGGGGAGGCAATGTTACATAACGACGCTTGAACATGAGGGGTCCATTTAACAGATTCAACCAGGGGCAAGTATGGTAAAGc                    >  2:3371928/1‑93 (MQ=255)
                                                                           gcaaCACAAAAAGGGGAGGCAATGTTACATAACGACGCTTGAACATGAGGGGTCCATTTAACAGATTCAACCAGGGGCAAGTATGGTAAAGCa                   <  1:5377518/93‑1 (MQ=255)
                                                                           gcaaCACAAAAAGGGGAAGCAATGTTACATAACGACGCTTGAACATGAGGGGTCCATTTAACAGATTCAACCAGGGGCAAGTATGGTAAAGCa                   >  2:1599261/1‑93 (MQ=255)
                                                                            caaCACAAAAAGGGGAGGCAATGTTACATAACGACGCTTGAACATGAGGGGTCCATTTAACAGATTCAACCAGGGGCAAGTATGGTAAAGCAt                  >  1:4913704/1‑93 (MQ=255)
                                                                              acacAAAAAGGGGAAGCAATGTTACATAACGACGCTTGAACATGAGGGGTCCATTTAACAGATTCAACCAGGGGCAAGTATGGTAAAGCATCa                <  2:27540/93‑1 (MQ=255)
                                                                                  aaaaaGGGGAGGCAATGTTACATAACGACGCTTGAACATGAGGGGTCCATTTAACAGATTCAACCAGGGGCAAGTATGGTAAAGCATCACGcc            >  2:6768434/1‑93 (MQ=255)
                                                                                   aaaaGGGGAGGCAATGTTACATAACGACGCTTGAACATGAGGGGTCCATTTAACAGATTCAACCAGGGGCAAGTATGGTAAAGCATCACGccc           <  1:51367/93‑1 (MQ=255)
                                                                                   aaaaGGGGAAGCAATGTTACATAACGACGCTTGAACATGAGGGGTCCATTTAACAGATTCAACCAGGGGCAAGTATGGTAAAGCATCACGccc           <  2:1289422/93‑1 (MQ=255)
                                                                                    aaaGGGGAGGCAATGTTACATAACGACGCTTGAACATGAGGGGTCCATTTAACAGATTCAACCAGGGGCAAGTATGGTAAAGCATCACGcccc          <  1:5949740/93‑1 (MQ=255)
                                                                                     aaGGGGAGGCAATGTTACATAACGACGCTTGAACATGAGGGGTCCATTTAACAGATTCAACCAGGGGCAAGTATGGTAAAGCATCACGCCCCg         >  1:2001786/1‑93 (MQ=255)
                                                                                     aaGGGGAGGCAATGTTACATAACGACGCTTGAACATGAGGGGTCCATTTAACAGATTCAACCAGGGGCAAGTATGGTAAAGCATCACGCCCCg         <  1:2750560/93‑1 (MQ=255)
                                                                                          gAAGCAATGTTACATAACGACGCTTGAACATGAGGGGTCCATTTAACAGATTCAACCAGGGGCAAGTATGGTAAAGCATCACGCCCCGCACaa    <  1:1016504/93‑1 (MQ=255)
                                                                                            aGCAATGTTACATAACGACGCTTGAACATGAGGGGTCCATTTAACAGATTCAACCAGGGGCAAGTATGGTAAAGCATCACGCCCCGCACAAgg  >  2:5768635/1‑93 (MQ=255)
                                                                                            |                                                                                            
GCAGGAAGCCGCCAGACGGCGTTCCGGTGGTCGTATCAGTCTCAGTAGGTTTTGGCTTGCTGCTACAGGCGGCAAGCAACACAAAAAGGGGAAGCAATGTTACATAACGACGCTTGAACATGAGGGGTCCATTTAACAGATTCAACCAGGGGCAAGTATGGTAAAGCATCACGCCCCGCACAAGG  >  NC_000913/2823479‑2823663

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AGCAGGAAGCCGCCAGACGGCGTTCCGGTGGTCGTATCAGTCTCAGTAGGTTTTGGCTTGCTGCTACAGGCGGCAAGCAACACAAAAAGGGGAAGCAATGTTACATAACGACGCTTGAACATGAGGGGTCCATTTAACAGATTCAACCAGGGGCAAGTATGGTAAAGCATCACGCCCCGCACAAGGA  >  NC_000913/2823478‑2823664
                                                                                             |                                                                                             
AGCAGGAAGCCGCCAGACGGCGTTCCGGTGGTCGTATCAGTCTCAGTAGGTTTTGGCTTGCTGCTACAGGCGGCAAGCAACACAAAAAGGGGAG                                                                                               <  SRR4302542.7217821/94‑1 (MQ=60)
AGCAGGAAGCCGCCAGACGGCGTTCCGGTGGTCGTATCAGTCTCAGTAGGTTTTGGCTTGCTGCTACAGGCGGCAAGCAACACAAAAAGGGGAG                                                                                               <  SRR4302542.5369134/94‑1 (MQ=60)
 GCAGGAAGCCGCCAGACGGCGTTCCGGTGGTCGTATCAGTCTCAGTAGGTTTTGGCTTGCTGCTACAGGCGGCAAGCAACACAAAAAGGGGAGG                                                                                              <  SRR4302542.2436233/94‑1 (MQ=60)
 GCAGGAAGCCGCCAGACGGCGTTCCGGTGGTCGTATCAGTCTCAGTAGGTTTTGGCTTGCTGCTACAGGCGGCAAGCAACACAAAAAGGGGAAG                                                                                              <  SRR4302542.3510704/94‑1 (MQ=60)
 GCAGGAAGCCGCCAGACGGCGTTCCGGTGGTCGTATCAGTCTCAGTAGGTTTTGGCTTGCTGCTACAGGCGGCAAGCAACACAAAAAGGGGAAG                                                                                              >  SRR4302542.7278634/1‑94 (MQ=60)
 GCAGGAAGCCGCCAGACGGCGTTCCGGTGGTCGTATCAGTCTCAGTAGGTTTTGGCTTGCTGCTACAGGCGGCAAGCAACACAAAAAGGGGAAG                                                                                              >  SRR4302542.819471/1‑94 (MQ=60)
  CAGGAAGCCGCCAGACGGCGTTCCGGTGGTCGTATCAGTCTCAGTAGGTTTTGGCTTGCTGCTACAGGCGGCAAGCAACACAAAAAGGGGAGGC                                                                                             <  SRR4302542.3540188/94‑1 (MQ=60)
  CAGGAAGCCGCCAGACGGCGTTCCGGTGGTCGTATCAGTCTCAGTAGGTTTTGGCTTGCTGCTACAGGCGGCAAGCAACACAAAAAGGGGAGGC                                                                                             <  SRR4302542.4545293/94‑1 (MQ=60)
   GGGAAGCCGCCAGACGGCGTTCCGGTGGTCGTATCACTCTCAGTATGTTTTGGCTTGCTGCTACAGGCGGCAAGCAACACAAAAAGGGGAGGCA                                                                                            <  SRR4302542.3625859/94‑1 (MQ=60)
   AGGAAGCCGCCAGACGGCGTTCCGGTGGTCGTATCAGTCTCAGTAGGTTTTGGCTTGCTGCTACAGGCGGCAAGCAACACAAAAAGGGGAGGCA                                                                                            >  SRR4302542.5583484/1‑94 (MQ=60)
   AGGAAGCCGCCAGACGGCGTTCCGGTGGTCGTATCAGTCTCAGTAGGTTTTGGCTTGCTGCTACAGGCGGCAAGCAACACAAAAAGGGGAGGCA                                                                                            >  SRR4302542.6727134/1‑94 (MQ=60)
   AGGAAGCCGCCAGACGGCGTTCCGGTGGTCGTATCAGTCTCAGTAGGTTTTGGCTTGCTGCTACAGGCGGCAAGCAACACAAAAAGGGGAGGCA                                                                                            >  SRR4302542.3166114/1‑94 (MQ=60)
   AGGAAGCCGCCAGACGGCGTTCCGGTGGTCGTATCAGTCTCAGTAGGTTTTGGCTTGCTGCTACAGGCGGCAAGCAACACAAAAAGGGGAGGCA                                                                                            >  SRR4302542.2687055/1‑94 (MQ=60)
     GAAGCCGCCAGACGGCGTTCCGGTGGTCGTATCAGTCTCAGTAGGTTTTGGCTTGCTGCTACAGGCGGCAAGCAACACAAAAAGGGGAGGCAAT                                                                                          <  SRR4302542.7147073/94‑1 (MQ=60)
      AAGCCGCCAGACGGCGTTCCGGTGGTCGTATCAGTCTCAGTAGGTTTTGGCTTGCTGCTACAGGCGGCAAGCAACACAAAAAGGGGAGGCAATG                                                                                         <  SRR4302542.3154213/94‑1 (MQ=60)
      AAGCCGCCAGACGGCGTTCCGGTGGTCGTATCAGTCTCAGTAGGTTTTGGCTTGCTGCTACAGGCGGCAAGCAACACAAAAAGGGGAAGCAATG                                                                                         >  SRR4302542.4281045/1‑94 (MQ=60)
       AGCCGCCAGACGGCGTTCCGGTGGTCGTATCAGTCTCAGTAGGTTTTGGCTTGCTGCTACAGGCGGCAAGCAACACAAAAAGGGGAGGCAATGT                                                                                        >  SRR4302542.459150/1‑94 (MQ=60)
        GCCGCCAGACGGCGTTCCGGTGGTCGTATCAGTCTCAGTAGGTTTTGGCTTGCTGCTACAGGCGGCAAGCAACACAAAAAGGGGAGGCAATGTT                                                                                       <  SRR4302542.4001100/94‑1 (MQ=60)
         CCGCCAGACGGCGTTCCGGTGGTCGTATCAGTCTCAGTAGGTTTTGGCTTGCTGCTACAGGCGGCAAGCAACACAAAAAGGGGAGGCAATGTTA                                                                                      <  SRR4302542.5523836/94‑1 (MQ=60)
         CCGCCAGACGGCGTTCCGGTGGTCGTATCAGTCTCAGTAGGTTTTGGCTTGCTGCTACAGGCGGCAAGCAACACAAAAAGGGGAGGCAATGTTA                                                                                      <  SRR4302542.5966942/94‑1 (MQ=60)
         CCGCCAGACGGCGTTCCGGTGGTCGTATCAGTCTCAGTAGGTTTTGGCTTGCTGCTACAGGCGGCAAGCAACACAAAAAGGGGAGGCAATGTTA                                                                                      >  SRR4302542.5259725/1‑94 (MQ=60)
          CGCCAGACGGCGTTCCGGTGGTCGTATCAGTCTCAGTAGGTTTTGGCTTGCTGCTACAGGCGGCAAGCAACACAAAAAGGGGAGGCAATGTTAC                                                                                     <  SRR4302542.5732544/94‑1 (MQ=60)
          CGCCAGACGGCGTTCCGGTGGTCGTATCAGTCTCAGTAGGTTTTGGCTTGCTGCTACAGGCGGCAAGCAACACAAAAAGGGGAGGCAATGTTAC                                                                                     >  SRR4302542.6068952/1‑94 (MQ=60)
           GCCAGACGGCGTTCCGGTGGTCGTATCAGTCTCAGTAGGTTTTGGCTTGCTGCTACAGGCGGCAAGCAACACAAAAAGGGGAGGCAATGTTACA                                                                                    <  SRR4302542.4654440/94‑1 (MQ=60)
           GCCAGACGGCGTTCCGGTGGTCGTATCAGTCTCAGTAGGTTTTGGCTTGCTGCTACAGGCGGCAAGCAACACAAAAAGGGGAGGCAATGTTACA                                                                                    <  SRR4302542.4913707/94‑1 (MQ=60)
           GCCAGACGGCGTTCCGGTGGTCGTATCAGTCTCAGTAGGTTTTGGCTTGCTGCTACAGGCGGCAAGCAACACAAAAAGGGGAAGCAATGTTACA                                                                                    >  SRR4302542.7465013/1‑94 (MQ=60)
            CCAGACGGCGTTCCGGTGGTCGTATCAGTCTCAGTAGGTTTTGGCTTGCTGCTACAGGCGGCAAGCAACACAAAAAGGGGAGGCAATGTTACAT                                                                                   <  SRR4302542.1590077/94‑1 (MQ=60)
             CAGACGGCGTTCCGGTGGTCGTATCAGTCTCAGTAGGTTTTGGCTTGCTGCTACAGGCGGCAAGCAACACAAAAAGGGGAAGCAATGTTACATA                                                                                  >  SRR4302542.6603556/1‑94 (MQ=60)
              TGACGGCGTTCCGGTGGTCGTATCAGTCTCAGTAGGTTTTGGCTTGCTGCTACAGGCGGCAAGCAACACAAAAAGGGGAAGCAATGTTACATAA                                                                                 >  SRR4302542.1704150/1‑94 (MQ=60)
              AGACGGCGTTCCGGTGGTCGTATCAGTCTCAGTAGGTTTTGGCTTGCTGCTACAGGCGGCAAGCAACACAAAAAGGGGAGGCAATGTTACATAA                                                                                 >  SRR4302542.449196/1‑94 (MQ=60)
               GACGGCGTTCCGGTGGTCGTATCAGTCTCAGTAGGTTTTGGCTTGCTGCTACAGGCGGCAAGCAACACAAAAAGGGGAAGCAATGTTACATAAC                                                                                <  SRR4302542.1134475/94‑1 (MQ=60)
                ACGGCGTTCCGGTGGTCGTATCAGTCTCAGTAGGTTTTGGCTTGCTGCTACAGGCGGCAAGCAACACAAAAAGGGGAGGCAATGTTACATAACG                                                                               <  SRR4302542.5674107/94‑1 (MQ=60)
                ACGGCGTTCCGGTGGTCGTATCAGTCTCAGTAGGTTTTGGCTTGCTGCTACAGGCGGCAAGCAACACAAAAAGGGGAAGCAATGTTACATAACG                                                                               <  SRR4302542.6764952/94‑1 (MQ=60)
                 CGGCGTTCCGGTGGTCGTATCAGTCTCAGTAGGTTTTGGCTTGCTGCTACAGGCGGCAAGCAACACAAAAAGGGGAGGCAATGTTACATAACGA                                                                              >  SRR4302542.6807628/1‑94 (MQ=60)
                 CGGCGTTCCGGTGGTCGTATCAGTCTCAGTAGGTTTTGGCTTGCTGCTACAGGCGGCAAGCAACACAAAAAGGGGAGGCAATGTTACATAACGA                                                                              >  SRR4302542.4757246/1‑94 (MQ=60)
                 CGGCGTTCCGGTGGTCGTATCAGTCTCAGTAGGTTTTGGCTTGCTGCTACAGGCGGCAAGCAACACAAAAAGGGGAGGCAATGTTACATAACGA                                                                              >  SRR4302542.4670258/1‑94 (MQ=60)
                  GGCGTTCCGGTGGTCGTATCAGTCTCAGTAGGTTTTGGCTTGCTGCTACAGGCGGCAAGCAACACAAAAAGGGGAGGCAATGTTACATAACGAC                                                                             <  SRR4302542.5261544/94‑1 (MQ=60)
                   GAGTTCCGGTGGTCGTATCAGTCTCAGTAGGTTTTGGCTTGCTGCTACAGGCGGCAAGCAACACAAAAAGGGGAGGCAATGTTACATAACGACG                                                                            >  SRR4302542.2016084/1‑94 (MQ=60)
                    CGTTCCGGTGGTCGTATCAGTCTCAGTAGGTTTTGGCTTGCTGCTACAGGCGGCAAGCAACACAAAAAGGGGAGGCAATGTTACATAACGACGC                                                                           >  SRR4302542.2803073/1‑94 (MQ=60)
                     GTTCCGGTGGTCGTATCAGTCTCAGTAGGTTTTGGCTTGCTGCTACAGGCGGCAAGCAACACAAAAAGGGGAGGCAATGTTACATAACGACGCT                                                                          >  SRR4302542.5215727/1‑94 (MQ=60)
                     GTTCCGGTGGTCGTATCAGTCTCAGTAGGTTTTGGCTTGCTGCTACAGGCGGCAAGCAACACAAAAAGGGGAGGCAATGTTACATAACGACGCT                                                                          >  SRR4302542.763308/1‑94 (MQ=60)
                     GTTCCGGTGGTCGTATCAGTCTCAGTAGGTTTTGGCTTGCTGCTACAGGCGGCAAGCAACACAAAAAGGGGAGGCAATGTTACATAACGACGCT                                                                          >  SRR4302542.1980165/1‑94 (MQ=60)
                     GTTCCGGTGGTCGCATCAGTCTCAGTAGGTTTTGGCTTGCTGCTACAGGCGGCAAGCAACACAAAAAGGGGAGGCAATGTTACATAACGACGCT                                                                          >  SRR4302542.4350124/1‑94 (MQ=60)
                      TTCCGGTGGTCGTATCAGTCTCAGTAGGTTTTGGCTTGCTGCTACAGGCGGCAAGCAACACAAAAAGGGGAAGCAATGTTACATAACGACGCTT                                                                         >  SRR4302542.3867517/1‑94 (MQ=60)
                        CCGGTGGTCGTATCAGTCTCAGTAGGTTTTGGCTTGCTGCTACAGGCGGCAAGCAACACAAAAAGGGGAGGCAATGTTACATAACGACGCTTGA                                                                       <  SRR4302542.4044883/94‑1 (MQ=60)
                         CGGTGGTCGTATCAGTCTCAGTAGGTTTTGGCTTGCTGCTACAGGCGGCAAGCAACACAAAAAGGGGAAGCAATGTTACATAACGACGCTTGAA                                                                      >  SRR4302542.1180258/1‑94 (MQ=60)
                          GGTGGTCGTATCAGTCTCAGTAGGTTTTGGCTTGCTGCTACAGGCGGCAAGCAACACAAAAAGGGGAGGCAATGTTACATAACGACGCTTGAAC                                                                     <  SRR4302542.5667238/94‑1 (MQ=60)
                           GTGGTCGTATCAGTCTCAGTAGGTTTTGGCTTGCTGCTACAGGCGGCAAGCAACACAAAAAGGGGAGGCAATGTTACATAACGACGCTTGAACA                                                                    <  SRR4302542.6724585/94‑1 (MQ=60)
                           GTGGTCGTATCAGTCTCAGTAGGTTTTGGCTTGCTGCTACAGGCGGCAAGCAACACAAAAAGGGGAGGCAATGTTACATAACGACGCTTGAACA                                                                    >  SRR4302542.2360098/1‑94 (MQ=60)
                            TGGTCGTATCAGTCTCAGTAGGTTTTGGCTTGCTGCTACAGGCGGCAAGCAACACAAAAAGGGGAGGCAATGTTACATAACGACGCTTGAACAT                                                                   <  SRR4302542.1236082/94‑1 (MQ=60)
                            TGGTCGTATCAGTCTCAGTAGGTTTTGGCTTGCTGCTACAGGCGGCAAGCAACACAAAAAGGGGAGGCAATGTTACATAACGACGCTTGAACAT                                                                   >  SRR4302542.2882900/1‑94 (MQ=60)
                            TGGTCGTATCAGTCTCAGTAGGTTTTGGCTTGCTGCTACAGGCGGCAAGCAACACAAAAAGGGGAAGCAATGTTACATAACGACGCTTGAACAT                                                                   <  SRR4302542.7441113/94‑1 (MQ=60)
                             GGTCGTATCAGTCTCAGTAGGTTTTGGCTTGCTGCTACAGGCGGCAAGCAACACAAAAAGGGGAGGCAATGTTACATAACGACGCTTGAACATG                                                                  <  SRR4302542.4904122/94‑1 (MQ=60)
                              GTCGTATCAGTCTCAGTAGGTTTTGGCTTGCTGCTACAGGCGGCAAGCAACACAAAAAGGGGAGGCAATGTTACATAACGACGCTTGAACATGA                                                                 <  SRR4302542.5110701/94‑1 (MQ=60)
                               TCGTATCAGTCTCAGTAGGTTTTGGCTTGCTGCTACAGGCGGCAAGCAACACAAAAAGGGGAAGCAATGTTACATAACGACGCTTGAACATGAG                                                                <  SRR4302542.707866/94‑1 (MQ=60)
                                CGTATCAGTCTCAGTAGGTTTTGGCTTGCTGCTACAGGCGGCAAGCAACACAAAAAGGGGAGGCAATGTTACATAACGACGCTTGAACATGAGG                                                               >  SRR4302542.6229458/1‑94 (MQ=60)
                                CGTATCAGTCTCAGTAGGTTTTGGCTTGCTGCTACAGGCGGCAAGCAACACAAAAAGGGGAGGCAATGTTACATAACGACGCTTGAACATGAGG                                                               >  SRR4302542.4869059/1‑94 (MQ=60)
                                 GTATCAGTCTCAGTAGGTTTTGGCTTGCTGCTACAGGCGGCAAGCAACACAAAAAGGGGAGGCAATGTTACATAACGATGCTTGAACATGAGGG                                                              <  SRR4302542.6068200/94‑1 (MQ=60)
                                 GTATCAGTCTCAGTAGGTTTTGGCTTGCTGCTACAGGCGGCAAGCAACACAAAAAGGGGAGGCAATGTTACATAACGACGCTTGAACATGAGGG                                                              >  SRR4302542.2545113/1‑94 (MQ=60)
                                 GTATCAGTCTCAGTAGGTTTTGGCTTGCTGCTACAGGCGGCAAGCAACACAAAAAGGGGAAGCAATGTTACATAACGACGCTTGAACATGAGGG                                                              <  SRR4302542.1822878/94‑1 (MQ=60)
                                   ATCAGTCTCAGTAGGTTTTGGCTTGCTGCTACAGGCGGCAAGCAACACAAAAAGGGGAGGCAATGTTACATAACGACGCTTGAACATGAGGGGT                                                            >  SRR4302542.5554431/1‑94 (MQ=60)
                                    TCAGTCTCAGTAGGTTTTGGCTTGCTGCTACAGGCGGCAAGCAACACAAAAAGGGGAGGCAATGTTACATAACGACGCTTGAACATGAGGGGTC                                                           <  SRR4302542.1247264/94‑1 (MQ=60)
                                    TCAGTCTCAGTAGGTTTTGGCTTGCTGCTACAGGCGGCAAGCAACACAAAAAGGGGAGGCAATGTTACATAACGACGCTTGAACATGAGGGGTC                                                           >  SRR4302542.3121143/1‑94 (MQ=60)
                                    TCAGTCTCAGTAGGTTTTGGCTTGCTGCTACAGGCGGCAAGCAACACAAAAAGGGGAAGCAATGTTACATAACGACGCTTGAACATGAGGGGTC                                                           <  SRR4302542.5683665/94‑1 (MQ=60)
                                     CAGTCTCAGTAGGTTTTGGCTTGCTGCTACAGGCGGCAAGCAACACAAAAAGGGGAGGCAATGTTACATAACGACGCTTGAACATGAGGGGTCC                                                          <  SRR4302542.940758/94‑1 (MQ=60)
                                      AGTCTCAGTAGGTTTTGGCTTGCTGCTACAGGCGGCAAGCAACACAAAAAGGGGAGGCAATGTTACATAACGACGCTTGAACATGAGGGGTCCA                                                         >  SRR4302542.4378420/1‑94 (MQ=60)
                                      AGTCTCAGTAGGTTTTGGCTTGCTGCTACAGGCGGCAAGCAACACAAAAAGGGGAGGCAATGTTACATAACGACGCTTGAACATGAGGGGTCCA                                                         <  SRR4302542.4931142/94‑1 (MQ=60)
                                         CTCAGTAGGTTTTGGCTTGCTGCTACAGGCGGCAAGCAACACAAAAAGGGGAGGCAATGTTACATAACGACGCTTGAACATGAGGGGTCCATTT                                                      >  SRR4302542.5574095/1‑94 (MQ=60)
                                           CAGTAGGTTTTGGCTTGCTGCTACAGGCGGCAAGCAACACCAAAAGGGGAGGCAATGTTACATAACGACGCTTGAACATGAGGGGTCCATTTAA                                                    >  SRR4302542.7585968/1‑94 (MQ=60)
                                           CAGTAGGTTTTGGCTTGCTGCTACAGGCGGCAAGCAACACAAAAAGGGGAGGCAATGTTACATAACGACGCTTGAACATGAGGGGTCCATTTAA                                                    <  SRR4302542.2729399/94‑1 (MQ=60)
                                           CAGTAGGTTTTGGCTTGCTGCTACAGGCGGCAAGCAACACAAAAAGGGGAGGCAATGTTACATAACGACGCTTGAACATGAGGGGTCCATTTAA                                                    >  SRR4302542.3843130/1‑94 (MQ=60)
                                            AGTAGGTTTTGGCTTGCTGCTACAGGCGGCAAGCAACACAAAAAGGGGAGGCAATGTTACATAACGACGCTTGAACATGAGGGGTCCATTTAAC                                                   >  SRR4302542.4170467/1‑94 (MQ=60)
                                            AGTAGGTTTTGGCTTGCTGCTACAGGCGGCAAGCAACACAAAAAGGGGAGGCAATGTTACATAACGACGCTTGAACATGAGGGGTCCATTTAAC                                                   >  SRR4302542.1923857/1‑94 (MQ=60)
                                             GTAGGTTTTGGCTTGCTGCTACAGGCGGCAAGCAACACAAAAAGGGGAGGCAATGTTACATAACGACGCTTGAACATGAGGGGTCCATTTAACA                                                  <  SRR4302542.2152441/94‑1 (MQ=60)
                                             GTAGGTTTTGGCTTGCTGCTACAGGCGGCAAGCAACACAAAAAGGGGAGGCAATGTTACATAACGACGCTTGAACATGAGGGGTCCATTTAACA                                                  <  SRR4302542.5930752/94‑1 (MQ=60)
                                              TAGGTTTTGGCTTGCTGCTACAGGCGGCAAGCAACACAAAAAGGGGAGGCAATGTTACATAACGACGCTTGAACATGAGGGGTCCATTTAACAG                                                 <  SRR4302542.5048907/94‑1 (MQ=60)
                                              TAGGTTTTGGCTTGCTGCTACAGGCGGCAAGCAACACAAAAAGGGGAAGCAATGTTACATAACGACGCTTGAACATGAGGGGTCCATTTAACAG                                                 <  SRR4302542.6371326/94‑1 (MQ=60)
                                               AGGTTTTGGCTTGCTGCTACAGGCGGCAAGCAACACAAAAAGGGGAGGCAATGTTACATAACGACGCTTGAACATGAGGGGTCCATTTAACAGA                                                <  SRR4302542.3580653/94‑1 (MQ=60)
                                               AGGTTTTGGCTTGCTGCTACAGGCGGCAAGCAACACAAAAAGGGGAGGCAATGTTACATAACGACGCTTGAACATGAGGGGTCCATTTAACAGA                                                >  SRR4302542.6425862/1‑94 (MQ=60)
                                               AGGTTTTGGCTTGCTGCTACAGGCGGCAAGCAACACAAAAAGGGGAGGCAATGTTACATAACGACGCTTGAACATGAGGGGTCCATTTAACAGA                                                <  SRR4302542.2715439/94‑1 (MQ=60)
                                                 GTTTTGGCTTGCTGCTACAGGCGGCAAGCAACACAAAAAGGGGAGGCAATGTTACATAACGACGCTTGAACATGAGGGGTCCATTTAACAGATT                                              <  SRR4302542.2387773/94‑1 (MQ=60)
                                                 GTTTTGGCTTGCTGCTACAGGCGGCAAGCAACACAAAAAGGGGAGGCAATGTTACATAACGACGCTTGAACATGAGGGGTCCATTTAACAGATT                                              <  SRR4302542.1837144/94‑1 (MQ=60)
                                                 GTTTTGGCTTGCTGCTACAGGCGGCAAGCAACACAAAAAGGGGAGGCAATGTTACATAACGACGCTTGAACATGAGGGGTCCATTTAACAGATT                                              <  SRR4302542.5194659/94‑1 (MQ=60)
                                                  TTTTGGCTTGCTGCTACAGGCGGCAAGCAACACAAAAAGGGGAGGCAATGTTACATAACGACGCTTGAACATGAGGGGTCCATTTAACAGATTC                                             <  SRR4302542.4491703/94‑1 (MQ=60)
                                                   TTTGGCTTGCTGCTACAGGCGGCAAGCAACACAAAAAGGGGAGGCAATGTTACATAACGACGCTTGAACATGAGGGGTCCATTTAACAGATTCA                                            <  SRR4302542.3246542/94‑1 (MQ=60)
                                                   TTTGGCTTGCTGCTACAGGCGGCAAGCAACACAAAAAGGGGAAGCAATGTTACATAACGACGCTTGAACATGAGGGGTCCATTTAACAGATTCA                                            <  SRR4302542.3235373/94‑1 (MQ=60)
                                                      GGCTTGCTGCTACAGGCGGCAAGCAACACAAAAAGGGGAGGCAATGTTACATAACGACGCTTGAACATGAGGGGTCCATTTAACAGATTTAACC                                         >  SRR4302542.1662936/1‑94 (MQ=60)
                                                      GGCTTGCTGCTACAGGCGGCAAGCAACACAAAAAGGGGAGGCAATGTTACATAACGACGCTTGAACATGAGGGGTCCATTTAACAGATTCAACC                                         <  SRR4302542.2428415/94‑1 (MQ=60)
                                                       GCTTGCTGCTACAGGCGGCAAGCAACACAAAAAGGGGAGGCAATGTTACATAACGACGCTTGAACATGAGGGGTCCATTTAACAGATTCAACCA                                        <  SRR4302542.6455302/94‑1 (MQ=60)
                                                       GCTTGCTGCTACAGGCGGCAAGCAACACAAAAAGGGGAGGCAATGTTACATAACGACGCTTGAACATGAGGGGTCCATTTAACAGATTCAACCA                                        <  SRR4302542.2885916/94‑1 (MQ=60)
                                                       GCTTGCTGCTACAGGCGGCAAGCAACACAAAAAGGGGAGGCAATGTTACATAACGACGCTTGAACATGAGGGGTCCATTTAACAGATTCAACCA                                        <  SRR4302542.5002327/94‑1 (MQ=60)
                                                        CTTGCTGCTACAGGCGGCAAGCAACACAAAAAGGGGAGGCAATGTTACATAACGACGCTTGAACATGAGGGGTCCATTTAACAGATTCAACCAG                                       <  SRR4302542.1134676/94‑1 (MQ=60)
                                                        CTTGCTGCTACAGGCGGCAAGCAACACAAAAAGGGGAGGCAATGTTACATAACGACGCTTGAACATGAGGGGTCCATTTAACAGATTCAACCAG                                       <  SRR4302542.3701766/94‑1 (MQ=60)
                                                        CTTGCTGCTACAGGCGGCAAGCAACACAAAAAGGGGAAGCAATGTTACATAACGACGCTTGAACATGAGGGGTCCATTTAACAGATTCAACCAG                                       >  SRR4302542.862932/1‑94 (MQ=60)
                                                         TTGCTGCTACAGGCGGCAAGCAACACAAAAAGGGGAGGCAATGTTACATAACGACGCTTGAACATGAGGGGTCCATTTAACAGATTCAACCAGG                                      <  SRR4302542.4891220/94‑1 (MQ=60)
                                                         TTGCTGCTACAGGCGGCAAGCAACACAAAAAGGGGAGGCAATGTTACATAACGACGCTTGAACATGAGGGGTCCATTTAACAGATTCAACCAGG                                      <  SRR4302542.132833/94‑1 (MQ=60)
                                                          TGCTGCTACAGGCGGCAAGCAACACAAAAAGGGGAGGCAATGTTACATAACGACGCTTGAACATGAGGGGTCCATTTAACAGATTCAACCAGGG                                     <  SRR4302542.5646508/94‑1 (MQ=60)
                                                          TGCTGCTACAGGCGGCAAGCAACACAAAAAGGGGAAGCAATGTTACATAACGACGCTTGAACATGAGGGGTCCATTTAACAGATTCAACCAGGG                                     <  SRR4302542.1679290/94‑1 (MQ=60)
                                                           GCTGCTACAGGCGGCAAGCAACACAAAAAGGGGAGGCAATGTTACATAACGACGCTTGAACATGAGGGGTCCATTTAACAGATTCAACCAGGGG                                    <  SRR4302542.4026299/94‑1 (MQ=60)
                                                           GCTGCTACAGGCGGCAAGCAACACAAAAAGGGGAGGCAATGTTACATAACGACGCTTGAACATGAGGGGTCCATTTAACAGATTCAACCAGGGG                                    <  SRR4302542.6608522/94‑1 (MQ=60)
                                                           GCTGCTACAGGCGGCAAGCAACACAAAAAGGGGAGGCAATGTTACATAACGACGCTTGAACATGAGGGGTCCATTTAACAGATTCAACCAGGGG                                    <  SRR4302542.5161348/94‑1 (MQ=60)
                                                            CTGCTACAGGCGGCAAGCAACACAAAAAGGGGAGGCAATGTTACATAACGACGCTTGAACATGAGGGGTCCATTTAACAGATTCAACCAGGGGC                                   >  SRR4302542.1796123/1‑94 (MQ=60)
                                                            CTGCTACAGGCGGCAAGCAACACAAAAAGGGGAAGCAATGTTACATAACGACGCTTGAACATGAGGGGTCCATTTAACAGATTCAACCAGGGGC                                   <  SRR4302542.4066839/94‑1 (MQ=60)
                                                              GCTACAGGCGGCAAGCAACACAAAAAGGGGAAGCAATGTTACATAACGACGCTTGAACATGAGGGGTCCATTTAACAGATTCAACCAGGGGCAA                                 >  SRR4302542.4770343/1‑94 (MQ=60)
                                                                 ACAGGCGGCAAGCAACACAAAAAGGGGAGGCAATGTTACATAACGACGCTTGAACATGAGGGGTCCATTTAACAGATTCAACCAGGGGCAAGTA                              <  SRR4302542.4563723/94‑1 (MQ=60)
                                                                  CAGGCGGCAAGCAACACAAAAAGGGGAGGCAATGTTACATAACGACGCTTGAACATGAGGGGTCCATTTAACAGATTCAACCAGGGGCAAGTAT                             >  SRR4302542.3531622/1‑94 (MQ=60)
                                                                   AGGCGGCAAGCAACACAAAAAGGGGAAGCAATGTTACATAACGACGCTTGAACATGAGGGGTCCATTTAACAGATTCAACCAGGGGCAAGTATG                            >  SRR4302542.6874752/1‑94 (MQ=60)
                                                                   AGGCGGCAAGCAACACAAAAAGGGGAAGCAATGTTACATAACGACGCTTGAACATGAGGGGTCCATTTAACAGATTCAACCAGGGGCAAGTATG                            >  SRR4302542.2794912/1‑94 (MQ=60)
                                                                    GGCGGCAAGCAACACAAAAAGGGGAGGCAATGTTACATAACGACGCTTGAACATGAGGGGTCCATTTAACAGATTCAACCAGGGGCAAGTATGG                           >  SRR4302542.3117984/1‑94 (MQ=60)
                                                                    GGCGGCAAGCAACACAAAAAGGGGAGGCAATGTTACATAACGACGCTTGAACATGAGGGGTCCATTTAACAGATTCAACCAGGGGCAAGTATGG                           <  SRR4302542.6759110/94‑1 (MQ=60)
                                                                       GGCAAGCAACACAAAAAGGGGAAGCAATGTTACATAACGACGCTTGAACATGAGGGGTCCATTTAACAGATTCAACCAGGGGCAAGTATGGTAA                        <  SRR4302542.6930997/94‑1 (MQ=60)
                                                                        GCAAGCAACACAAAAAGGGGAGGCAATGTTACATAACGACGCTTGAACATGAGGGGTCCATTTAACAGATTCAACCAGGGGCAAGTATGGTAAA                       >  SRR4302542.6770517/1‑94 (MQ=60)
                                                                          AAGCAACACAAAAAGGGGAGGCAATGTTACATAACGACGCTTGAACATGAGGGGTCCATTTAACAGATTCAACCAGGGGCAAGTATGGTAAAGC                     >  SRR4302542.79744/1‑94 (MQ=60)
                                                                           AGCAACACAAAAAGGGGAGGCAATGTTACATAACGACGCTTGAACATGAGGGGTCCATTTAACAGATTCAACCAGGGGCAAGTATGGTAAAGCA                    <  SRR4302542.2628651/94‑1 (MQ=60)
                                                                           AGCAACACAAAAAGGGGAGGCAATGTTACATAACGACGCTTGAACATGAGGGGTCCATTTAACAGATTCAACCAGGGGCAAGTATGGTAAAGCA                    >  SRR4302542.6495814/1‑94 (MQ=60)
                                                                            GCAACACAAAAAGGGGAGGCAATGTTACATAACGACGCTTGAACATGAGGGGTCCATTTAACAGATTCAACCAGGGGCAAGTATGGTAAAGCAT                   >  SRR4302542.4961494/1‑94 (MQ=60)
                                                                            GCAACACAAAAAGGGGAGGCAATGTTACATAACGACGCTTGAACATGAGGGGTCCATTTAACAGATTCAACCAGGGGCAAGTATGGTAAAGCAT                   <  SRR4302542.5429766/94‑1 (MQ=60)
                                                                              AACACAAAAAGGGGAGGCAATGTTACATAACGACGCTTGAACATGAGGGGTCCATTTAACAGATTCAACCAGGGGCAAGTATGGTAAAGCATCA                 >  SRR4302542.5864830/1‑94 (MQ=60)
                                                                               ACACAAAAAGGGGAGGCAATGTTACATAACGACGCTTGAACATGAGGGGTCCATTTAACAGATTCAACCAGGGGCAAGTATGGTAAAGCATCAC                >  SRR4302542.2590331/1‑94 (MQ=60)
                                                                               ACACAAAAAGGGGAGGCAATGTTACATAACGACGCTTGAACATGAGGGGTCCATTTAACAGATTCAACCAGGGGCAAGTATGGTAAAGCATCAC                <  SRR4302542.6774052/94‑1 (MQ=60)
                                                                               ACACAAAAAGGGGAGGCAATGTTACATAACGACGCTTGAACATGAGGGGTCCATTTAACAGATTCAACCAGGGGCAAGTATGGTAAAGCATCAC                <  SRR4302542.3316722/94‑1 (MQ=60)
                                                                               ACACAAAAAGGGGAGGCAATGTTACATAACGACGCTTGAACATGAGGGGGCCATTTAACAGATTCAACCAGGGGCAAGTATGGTAAAGCATCAC                >  SRR4302542.79110/1‑94 (MQ=60)
                                                                                CACAAAAAGGGGAGGCAATGTTACATAACGACGCTTGAACATGAGGGGTCCATTTAACAGATTCAACCAGGGGCAAGTATGGTAAAGCATCACG               <  SRR4302542.7284512/94‑1 (MQ=60)
                                                                                AACAAAAAGGGGAAGCAATTTTACAAAACGGCGCTTAAAAATGGGGGGTCCATTTAACAGATTCAACCAGGGGCAAGTATGGTAAAGCATCACC               <  SRR4302542.2403444/94‑1 (MQ=60)
                                                                                  CAAAAAGGGGAGGCAATGTTACATAACGACGCTTGAACATGAGGGGTCCATTTAACAGATTCAACCAGGGGCAAGTATGGTAAAGCATCACGCC             <  SRR4302542.366076/94‑1 (MQ=60)
                                                                                   AAAAAGGGGAGGCAATGTTACATAACGACGCTTGAACATTAGGGGTCCATTTAACAGATTCAACCAGGGGCAAGTATGGTAAAGCATCACGCCC            >  SRR4302542.4479763/1‑94 (MQ=60)
                                                                                    AAAAGGGGAGGCAATGTTACATAACGACGCTTGAACATGAGGGGTCCATTTAACAGATTCAACCAGGGGCAAGTATGGTAAAGCATCACGCCCC           <  SRR4302542.51780/94‑1 (MQ=60)
                                                                                    AAAAGGGGAAGCAATGTTACATAACGACGCTTGAACATGAGGGGTCCATTTAACAGATTCAACCAGGGGCAAGTATGGTAAAGCATCACGCCCC           >  SRR4302542.1060687/1‑94 (MQ=60)
                                                                                     AAAGGGGAGGCAATGTTACATAACGACGCTTGAACATGAGGGGTCCATTTAACAGATTCAACCAGGGGCAAGTATGGTAAAGCATCACGCCCCG          <  SRR4302542.4379640/94‑1 (MQ=60)
                                                                                     AAAGGGGAGGCAATGTTACATAACGACGCTTGAACATGAGGGGTCCATTTAACAGATTCAACCAGGGGCAAGTATGGTAAAGCATCACGCCCCG          >  SRR4302542.2019071/1‑94 (MQ=60)
                                                                                     AAAGGGGAGGCAATGTTACATAACGACGCTTGAACATGAGGGGTCCATTTAACAGATTCAACCAGGGGCAAGTATGGTAAAGCATCACGCCCCG          <  SRR4302542.6007362/94‑1 (MQ=60)
                                                                                      AAGGGGAGGCAATGTTACATAACGACGCTTGAACATGAGGGGTCCATTTAACAGATTCAACCAGGGGCAAGTATGGTAAAGCATCACGCCCCGC         <  SRR4302542.2777072/94‑1 (MQ=60)
                                                                                      AAGGGGAGGCAATGTTACATAACGACGCTTGAACATGAGGGGTCCATTTAACAGATTCAACCAGGGGCAAGTATGGTAAAGCATCACGCCCCGC         >  SRR4302542.1472225/1‑94 (MQ=60)
                                                                                       AGGGGAGGCAATGTTACATAACGACGCTTGAACATGAGGGGTCCATTTAACAGATTCAACCAGGGGCAAGTATGGTAAAGCATCACGCCCCGCA        >  SRR4302542.4619807/1‑94 (MQ=60)
                                                                                        GGGGAGGCAATGTTACATAACGACGCTTGAACATGAGGGGTCCATTTAACAGATTCAACCAGGGGCAAGTATGGTAAAGCATCACGCCCCGCAC       <  SRR4302542.1500538/94‑1 (MQ=60)
                                                                                         GGGAGGCAATGTTACATAACGACGCTTGAACATGAGGGGTCCATTTAACAGATTCAACCAGGGGCAAGTATGGTAAAGCATCACGCCCCGCACA      <  SRR4302542.3684299/94‑1 (MQ=60)
                                                                                          GGAAGCAATGTTACATAACGACGCTTGAACATGAGGGGTCCATTTAACAGATTCAACCAGGGGCAAGTATGGTAAAGCATCACGCCCCGCACAA     <  SRR4302542.4792418/94‑1 (MQ=60)
                                                                                           GAAGCAATGTTACATAACGACGCTTGAACATGAGGGGTCCATTTAACAGATTCAACCAGGGGCAAGTATGGTAAAGCATCACGCCCCGCACAAG    <  SRR4302542.1025259/94‑1 (MQ=60)
                                                                                            AGGCAATGTTACATAACGACGCTTGAACATGAGGGGTCCATTTAACAGATTCAACCAGGGGCAAGTATGGTAAAGCATCACGCCCCGCACAAGG   <  SRR4302542.4407062/94‑1 (MQ=60)
                                                                                            AAGCAATGTTAAATAACGACGCTTGAACATGAGGGGTCCATTTAACAGATTCAACCAGGGGCAAGTATGGTAAAGCATCACGCCCCGCACAAGG   <  SRR4302542.2723337/94‑1 (MQ=60)
                                                                                             GGCAATGTTACATAACGACGCTTGAACATGAGGGGTCCATTTAACAGATTCAACCAGGGGCAAGTATGGTAAAGCATCACGCCCCGCACAAGGA  <  SRR4302542.5654885/94‑1 (MQ=60)
                                                                                             |                                                                                             
AGCAGGAAGCCGCCAGACGGCGTTCCGGTGGTCGTATCAGTCTCAGTAGGTTTTGGCTTGCTGCTACAGGCGGCAAGCAACACAAAAAGGGGAAGCAATGTTACATAACGACGCTTGAACATGAGGGGTCCATTTAACAGATTCAACCAGGGGCAAGTATGGTAAAGCATCACGCCCCGCACAAGGA  >  NC_000913/2823478‑2823664

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑