Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1061 294.1 15065038 98.6% 14854127 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,824,797 T→C F128S (TTT→TCT)  srlE → PTS system glucitol/sorbitol‑specific enzyme IIBC component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,824,7970TC100.0% 937.1 / NA 275F128S (TTT→TCT) srlEPTS system glucitol/sorbitol‑specific enzyme IIBC component
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (163/112);  total (163/112)

Only 100 of 277 total aligned reads displayed.

CGCTGGCGCAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTTTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATGGGGTCCA  >  NC_000913/2824707‑2824886
                                                                                          |                                                                                         
cgCTGGCGCAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCtctt                                                                                         >  1:5925933/1‑93 (MQ=255)
cgCTGGCGCAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCtctt                                                                                         >  2:4982273/1‑93 (MQ=255)
 gCTGGCGCAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTc                                                                                        <  2:7279240/93‑1 (MQ=255)
 gCTGGCGCAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTc                                                                                        >  1:5832045/1‑93 (MQ=255)
   tGGCGCAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCg                                                                                      >  1:6987621/1‑93 (MQ=255)
     gcgcAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTg                                                                                    >  1:5183962/1‑93 (MQ=255)
     gcgcAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTg                                                                                    <  2:1823124/93‑1 (MQ=255)
      cgcAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTgg                                                                                   <  2:1356915/93‑1 (MQ=255)
       gcAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTggg                                                                                  >  2:3285140/1‑93 (MQ=255)
       gcAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTggg                                                                                  >  1:4562124/1‑93 (MQ=255)
       gcAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTggg                                                                                  <  2:578896/93‑1 (MQ=255)
         aGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGcc                                                                                <  1:188727/93‑1 (MQ=255)
           tACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGt                                                                              >  2:937670/1‑93 (MQ=255)
            aCATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTg                                                                             <  1:2113136/93‑1 (MQ=255)
             cATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGa                                                                            >  2:2004532/1‑93 (MQ=255)
             cATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGa                                                                            >  2:1578553/1‑93 (MQ=255)
             cATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGa                                                                            <  2:843018/93‑1 (MQ=255)
               ttGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACt                                                                          >  1:1610520/1‑93 (MQ=255)
                tgtgGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGGCACCACAACCCTCTTCCGTGGGCCGTGACTa                                                                         <  1:5697639/93‑1 (MQ=255)
                tgtgGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTa                                                                         <  2:4439082/93‑1 (MQ=255)
                  tgGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATg                                                                       >  1:7398509/1‑93 (MQ=255)
                     aaGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGaca                                                                    >  1:5129043/1‑93 (MQ=255)
                     aaGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGaca                                                                    <  2:6007747/93‑1 (MQ=255)
                      aGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGacac                                                                   >  1:6749162/1‑93 (MQ=255)
                         tatTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAg                                                                >  1:2561025/1‑93 (MQ=255)
                         tatTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAg                                                                >  2:5205646/1‑93 (MQ=255)
                          atTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGt                                                               >  1:3253677/1‑93 (MQ=255)
                          atTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGt                                                               >  2:712387/1‑93 (MQ=255)
                           tttATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTa                                                              >  2:4235553/1‑93 (MQ=255)
                             tATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAg                                                            >  1:3783541/1‑93 (MQ=255)
                             tATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAg                                                            >  2:2473184/1‑93 (MQ=255)
                             tATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAg                                                            >  1:1093175/1‑93 (MQ=255)
                             tATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAg                                                            <  1:5334107/93‑1 (MQ=255)
                              aTGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGa                                                           <  2:5701229/93‑1 (MQ=255)
                               tGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGaa                                                          >  1:5664663/1‑93 (MQ=255)
                               tGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGaa                                                          >  1:6622320/1‑93 (MQ=255)
                               tGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGaa                                                          >  2:2258163/1‑93 (MQ=255)
                                gTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGaaa                                                         <  2:3078849/93‑1 (MQ=255)
                                 tctcTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGaaaa                                                        >  1:7174938/1‑93 (MQ=255)
                                 tctcTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGaaaa                                                        >  2:7514594/1‑93 (MQ=255)
                                  ctctGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAAt                                                       >  2:2622393/1‑93 (MQ=255)
                                  ctctGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAAt                                                       >  1:6855274/1‑93 (MQ=255)
                                  ctctGGCGTAAAAGAAGAAAACATCACTGTAGTAGGGGATGGGACACCACAACCCTCTTCCGTGGGCGGGGATTATGACACCGGTAAGAAAAt                                                       >  1:1310372/1‑93 (MQ=255)
                                     tGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCAc                                                    <  1:3722702/93‑1 (MQ=255)
                                     tGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCAc                                                    <  2:3701384/93‑1 (MQ=255)
                                     tGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCAc                                                    <  2:5112187/93‑1 (MQ=255)
                                     tGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCAc                                                    >  2:1694077/1‑93 (MQ=255)
                                       gCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCg                                                  >  1:4614606/1‑93 (MQ=255)
                                         gTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGaa                                                >  1:6471269/1‑93 (MQ=255)
                                         gTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGaa                                                >  1:2395012/1‑93 (MQ=255)
                                          tAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAAc                                               >  1:1787094/1‑93 (MQ=255)
                                           aaaaGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACa                                              <  1:2746553/93‑1 (MQ=255)
                                           aaaaGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACa                                              >  2:2403172/1‑93 (MQ=255)
                                           aaaaGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACa                                              <  2:394627/93‑1 (MQ=255)
                                             aagaagAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACaaa                                            <  2:1493064/93‑1 (MQ=255)
                                             aagaagAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACaaa                                            <  1:818335/93‑1 (MQ=255)
                                             aagaagAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACaaa                                            >  1:6699182/1‑93 (MQ=255)
                                              agaagaAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAg                                           <  1:3050592/93‑1 (MQ=255)
                                              agaagaAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAg                                           >  2:3596522/1‑93 (MQ=255)
                                               gaagaaAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGc                                          <  2:1180186/93‑1 (MQ=255)
                                               gaagaaAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGc                                          <  2:3868366/93‑1 (MQ=255)
                                                 agaaAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCTTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGa                                        <  1:6222725/93‑1 (MQ=255)
                                                   aaaaCATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATg                                      >  1:7483839/1‑93 (MQ=255)
                                                    aaaCATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATgg                                     >  2:7202170/1‑93 (MQ=255)
                                                    aaaCATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATgg                                     >  2:3481547/1‑93 (MQ=255)
                                                      aCATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGtt                                   <  2:2159402/93‑1 (MQ=255)
                                                        aTCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTa                                 >  2:4716476/1‑93 (MQ=255)
                                                         tCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTAc                                >  2:893882/1‑93 (MQ=255)
                                                          cACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACt                               <  2:6113976/93‑1 (MQ=255)
                                                          cACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACt                               <  1:4281957/93‑1 (MQ=255)
                                                            cTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTgg                             <  1:1128955/93‑1 (MQ=255)
                                                             tGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGc                            <  2:7041621/93‑1 (MQ=255)
                                                             tGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGc                            <  2:5546570/93‑1 (MQ=255)
                                                              gtagtaGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCg                           <  1:2509433/93‑1 (MQ=255)
                                                              gtagtaGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCg                           >  2:4894219/1‑93 (MQ=255)
                                                                agtagGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGaa                         >  2:184760/1‑93 (MQ=255)
                                                                 gtagGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAg                        <  1:4138559/93‑1 (MQ=255)
                                                                 gtagGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAg                        >  2:1077242/1‑93 (MQ=255)
                                                                    ggTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTg                     <  1:6044859/93‑1 (MQ=255)
                                                                    ggTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTg                     <  1:6280481/93‑1 (MQ=255)
                                                                    ggTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTg                     <  1:3596822/93‑1 (MQ=255)
                                                                     gTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTgg                    >  2:6610812/1‑93 (MQ=255)
                                                                     gTGATGCGACACCACAACCCTCTTCCGTGGGCCGTAACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTgg                    >  2:4525887/1‑93 (MQ=255)
                                                                      tGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTggg                   >  2:4150016/1‑93 (MQ=255)
                                                                       gATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGa                  <  2:2863652/93‑1 (MQ=255)
                                                                       gATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGa                  >  2:6405192/1‑93 (MQ=255)
                                                                        aTGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGat                 <  1:3314424/93‑2 (MQ=255)
                                                                        aTGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGaa                 <  1:3860193/93‑1 (MQ=255)
                                                                        aTGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGaa                 >  1:4447663/1‑93 (MQ=255)
                                                                          gCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATg               <  2:6275635/93‑1 (MQ=255)
                                                                           cGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATgg              <  1:487817/93‑1 (MQ=255)
                                                                            gACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATggg             >  2:5466387/1‑93 (MQ=255)
                                                                             acacCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGc            >  1:3101530/1‑93 (MQ=255)
                                                                             acacCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGc            >  2:6836006/1‑93 (MQ=255)
                                                                              caccacAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCa           <  2:3373794/93‑1 (MQ=255)
                                                                               accacAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCAt          <  2:292732/93‑1 (MQ=255)
                                                                                 cacaACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATgg        >  2:541828/1‑93 (MQ=255)
                                                                                  acaACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATggg       >  2:5102483/1‑93 (MQ=255)
                                                                                   caACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATgggg      <  2:4002098/93‑1 (MQ=255)
                                                                                    aaCCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATGGGGt     <  1:4477994/93‑1 (MQ=255)
                                                                                          |                                                                                         
CGCTGGCGCAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTTTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATGGGGTCCA  >  NC_000913/2824707‑2824886

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TCCGCTGGCGCAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTTTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATGGGGTCCACCGT  >  NC_000913/2824705‑2824890
                                                                                            |                                                                                             
TCCGCTGGCGCAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCT                                                                                              <  SRR4302542.6797847/94‑1 (MQ=60)
 CCGCTGGCGCAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTT                                                                                             >  SRR4302542.5983356/1‑94 (MQ=60)
  CGCTGGCGCAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTC                                                                                            >  SRR4302542.5888634/1‑94 (MQ=60)
    CTGGCGCAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCG                                                                                          >  SRR4302542.7056258/1‑94 (MQ=60)
      GGCGCAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTG                                                                                        >  SRR4302542.5234331/1‑94 (MQ=60)
       GCGCAGTACATTGTGGAAGATATTTATGTCCCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGG                                                                                       >  SRR4302542.1150261/1‑94 (MQ=60)
        CGCAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGG                                                                                      <  SRR4302542.6992428/94‑1 (MQ=60)
        CGCAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGG                                                                                      <  SRR4302542.7234957/94‑1 (MQ=60)
        CGCAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGG                                                                                      <  SRR4302542.1661772/94‑1 (MQ=60)
        CGCAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGG                                                                                      >  SRR4302542.6528761/1‑94 (MQ=60)
        CGCAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGG                                                                                      >  SRR4302542.4606794/1‑94 (MQ=60)
         GCAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGC                                                                                     >  SRR4302542.5944134/1‑94 (MQ=60)
          CAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCC                                                                                    <  SRR4302542.2770609/94‑1 (MQ=60)
          CAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCC                                                                                    >  SRR4302542.3751195/1‑94 (MQ=60)
          CAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCC                                                                                    >  SRR4302542.6729192/1‑94 (MQ=60)
           AGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCG                                                                                   <  SRR4302542.190327/94‑1 (MQ=60)
           AGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCG                                                                                   >  SRR4302542.2435975/1‑94 (MQ=60)
             TACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTG                                                                                 >  SRR4302542.2654480/1‑94 (MQ=60)
             TACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTG                                                                                 >  SRR4302542.4784861/1‑94 (MQ=60)
              ACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGA                                                                                <  SRR4302542.2131374/94‑1 (MQ=60)
               CATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGAC                                                                               >  SRR4302542.4028715/1‑94 (MQ=60)
                ATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACT                                                                              >  SRR4302542.666575/1‑94 (MQ=60)
                ATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACT                                                                              >  SRR4302542.1624428/1‑94 (MQ=60)
                ATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACT                                                                              >  SRR4302542.4567426/1‑94 (MQ=60)
                  TGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGGCACCACAACCCTCTTCCGTGGGCCGTGACTAT                                                                            <  SRR4302542.5752946/94‑1 (MQ=60)
                  TGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTAT                                                                            <  SRR4302542.927425/94‑1 (MQ=60)
                  TGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTAT                                                                            <  SRR4302542.6328515/94‑1 (MQ=60)
                  TGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTAT                                                                            >  SRR4302542.4933102/1‑94 (MQ=60)
                   GTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATG                                                                           >  SRR4302542.7470915/1‑94 (MQ=60)
                   GTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATG                                                                           >  SRR4302542.7184469/1‑94 (MQ=60)
                    TGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAAGCCTCTTCCGTGGGCCGTGACTATGA                                                                          >  SRR4302542.3000311/1‑94 (MQ=60)
                    TGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGA                                                                          >  SRR4302542.6790386/1‑94 (MQ=60)
                    TGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGA                                                                          >  SRR4302542.1632414/1‑94 (MQ=60)
                      GAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACA                                                                        >  SRR4302542.5178858/1‑94 (MQ=60)
                       AAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACAC                                                                       <  SRR4302542.2057992/94‑1 (MQ=60)
                       AAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACAC                                                                       >  SRR4302542.6815572/1‑94 (MQ=60)
                        AGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACC                                                                      >  SRR4302542.6876529/1‑94 (MQ=60)
                         GATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCA                                                                     <  SRR4302542.378613/94‑1 (MQ=60)
                          ATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAG                                                                    >  SRR4302542.2585847/1‑94 (MQ=60)
                          ATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAG                                                                    >  SRR4302542.5602126/1‑94 (MQ=60)
                          ATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGCAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAG                                                                    <  SRR4302542.4499819/94‑1 (MQ=60)
                           TATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGT                                                                   >  SRR4302542.3284408/1‑94 (MQ=60)
                           TATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGT                                                                   >  SRR4302542.3028915/1‑94 (MQ=60)
                           TATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAATAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGT                                                                   >  SRR4302542.6278892/1‑94 (MQ=60)
                            ATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTA                                                                  >  SRR4302542.744475/1‑94 (MQ=60)
                              TTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAG                                                                >  SRR4302542.2444159/1‑94 (MQ=60)
                              TTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAG                                                                >  SRR4302542.1102543/1‑94 (MQ=60)
                              TTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAG                                                                >  SRR4302542.3820969/1‑94 (MQ=60)
                               TATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGA                                                               <  SRR4302542.5385935/94‑1 (MQ=60)
                                ATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAA                                                              >  SRR4302542.5719685/1‑94 (MQ=60)
                                ATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAA                                                              >  SRR4302542.661669/1‑94 (MQ=60)
                                ATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAA                                                              <  SRR4302542.1129766/94‑1 (MQ=60)
                                ATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAA                                                              >  SRR4302542.6687612/1‑94 (MQ=60)
                                 TGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAA                                                             >  SRR4302542.536793/1‑94 (MQ=60)
                                  GTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAA                                                            >  SRR4302542.7245324/1‑94 (MQ=60)
                                   TCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAAT                                                           >  SRR4302542.6922648/1‑94 (MQ=60)
                                   TCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGGGATGGGACACCACAACCCTCTTCCGTGGGCGGGGATTATGACACCGGTAAGAAAAT                                                           >  SRR4302542.1321663/1‑94 (MQ=60)
                                     TCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCA                                                         >  SRR4302542.70313/1‑94 (MQ=60)
                                      CTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCAC                                                        >  SRR4302542.3089786/1‑94 (MQ=60)
                                      CTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCAC                                                        <  SRR4302542.3621248/94‑1 (MQ=60)
                                       TGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACC                                                       <  SRR4302542.3759574/94‑1 (MQ=60)
                                       TGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACC                                                       <  SRR4302542.5905524/94‑1 (MQ=60)
                                        GGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCG                                                      >  SRR4302542.4659691/1‑94 (MQ=60)
                                          CGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAA                                                    >  SRR4302542.2418026/1‑94 (MQ=60)
                                          CGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAA                                                    >  SRR4302542.6535029/1‑94 (MQ=60)
                                           GTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAAC                                                   >  SRR4302542.6724623/1‑94 (MQ=60)
                                           GTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAAC                                                   >  SRR4302542.1802605/1‑94 (MQ=60)
                                             AAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAA                                                 >  SRR4302542.4597171/1‑94 (MQ=60)
                                             AAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAA                                                 <  SRR4302542.1327576/94‑1 (MQ=60)
                                             AAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAA                                                 <  SRR4302542.2773036/94‑1 (MQ=60)
                                              AAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAA                                                >  SRR4302542.6765195/1‑94 (MQ=60)
                                               AAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAG                                               <  SRR4302542.5729389/94‑1 (MQ=60)
                                               AAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAG                                               <  SRR4302542.1221086/94‑1 (MQ=60)
                                               AAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAG                                               >  SRR4302542.3227257/1‑94 (MQ=60)
                                               AAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAG                                               <  SRR4302542.825408/94‑1 (MQ=60)
                                                AGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGC                                              >  SRR4302542.4242314/1‑94 (MQ=60)
                                                AGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGC                                              <  SRR4302542.3079712/94‑1 (MQ=60)
                                                  AAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGA                                            >  SRR4302542.5062461/1‑94 (MQ=60)
                                                   AGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCTTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGAT                                           <  SRR4302542.6284009/94‑1 (MQ=60)
                                                    GAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATG                                          <  SRR4302542.444031/94‑1 (MQ=60)
                                                    GAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATG                                          >  SRR4302542.7557208/1‑94 (MQ=60)
                                                     AAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGG                                         >  SRR4302542.255415/1‑94 (MQ=60)
                                                      AAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGT                                        <  SRR4302542.6478352/94‑1 (MQ=60)
                                                       AACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTT                                       >  SRR4302542.537120/1‑94 (MQ=60)
                                                       AACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTT                                       <  SRR4302542.774521/94‑1 (MQ=60)
                                                        ACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTT                                      <  SRR4302542.5649426/94‑1 (MQ=60)
                                                         CATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTA                                     >  SRR4302542.3292681/1‑94 (MQ=60)
                                                          ATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTAC                                    >  SRR4302542.612555/1‑94 (MQ=60)
                                                          ATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTAC                                    <  SRR4302542.4648111/94‑1 (MQ=60)
                                                          ATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTAC                                    <  SRR4302542.3347717/94‑1 (MQ=60)
                                                          ATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTAC                                    >  SRR4302542.3121492/1‑94 (MQ=60)
                                                           TCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACT                                   >  SRR4302542.3269999/1‑94 (MQ=60)
                                                            CACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTG                                  <  SRR4302542.4324078/94‑1 (MQ=60)
                                                            CACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTG                                  >  SRR4302542.6011997/1‑94 (MQ=60)
                                                            CACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTG                                  >  SRR4302542.6064369/1‑94 (MQ=60)
                                                             ACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGG                                 >  SRR4302542.4417519/1‑94 (MQ=60)
                                                             ACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGG                                 <  SRR4302542.5741993/94‑1 (MQ=60)
                                                             ACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGG                                 <  SRR4302542.7481689/94‑1 (MQ=60)
                                                              CTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGC                                <  SRR4302542.1138589/94‑1 (MQ=60)
                                                               TGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCG                               >  SRR4302542.4781088/1‑94 (MQ=60)
                                                                GTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGA                              <  SRR4302542.2533782/94‑1 (MQ=60)
                                                                GTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGA                              >  SRR4302542.6772269/1‑94 (MQ=60)
                                                                GTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGA                              <  SRR4302542.1815214/94‑1 (MQ=60)
                                                                 TAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAA                             <  SRR4302542.4038510/94‑1 (MQ=60)
                                                                  AGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAG                            <  SRR4302542.2053024/94‑1 (MQ=60)
                                                                   GTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGG                           <  SRR4302542.4179334/94‑1 (MQ=60)
                                                                   GTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGG                           <  SRR4302542.3878358/94‑1 (MQ=60)
                                                                      GGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGG                        <  SRR4302542.6342370/94‑1 (MQ=60)
                                                                      GGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGG                        <  SRR4302542.3630360/94‑1 (MQ=60)
                                                                      GGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGG                        <  SRR4302542.6103371/94‑1 (MQ=60)
                                                                       GTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGG                       <  SRR4302542.465401/94‑1 (MQ=60)
                                                                        TGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGA                      <  SRR4302542.3695963/94‑1 (MQ=60)
                                                                         GATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAA                     >  SRR4302542.4491292/1‑94 (MQ=60)
                                                                          ATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGATT                    <  SRR4302542.3345663/94‑1 (MQ=60)
                                                                          ATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAAT                    >  SRR4302542.4288976/1‑94 (MQ=60)
                                                                          ATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAAT                    <  SRR4302542.3898317/94‑1 (MQ=60)
                                                                           TGCGACACCACACCCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATG                   <  SRR4302542.959610/94‑1 (MQ=60)
                                                                           TGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATG                   <  SRR4302542.5200373/94‑1 (MQ=60)
                                                                             CGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGG                 <  SRR4302542.492036/94‑1 (MQ=60)
                                                                              GACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGC                >  SRR4302542.3131000/1‑94 (MQ=60)
                                                                               ACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCA               >  SRR4302542.2163655/1‑94 (MQ=60)
                                                                                CACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCAT              >  SRR4302542.1521318/1‑94 (MQ=60)
                                                                                 ACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATG             >  SRR4302542.785817/1‑94 (MQ=60)
                                                                                   CACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATGGG           >  SRR4302542.2169836/1‑94 (MQ=60)
                                                                                      AACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATGGGGTC        >  SRR4302542.5862349/1‑94 (MQ=60)
                                                                                      AACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATGGGGTC        <  SRR4302542.4521954/94‑1 (MQ=60)
                                                                                      AACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATGGGGTC        <  SRR4302542.3795660/94‑1 (MQ=60)
                                                                                        CCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATGGGGTCCA      >  SRR4302542.7274573/1‑94 (MQ=60)
                                                                                          CTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATGGGGTCCACC    >  SRR4302542.834573/1‑94 (MQ=60)
                                                                                           TCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATGGGGTCCACCG   >  SRR4302542.2465956/1‑94 (MQ=60)
                                                                                           TCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATGGGGTCCACCG   >  SRR4302542.2470892/1‑94 (MQ=60)
                                                                                           TCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATGGGGTCCACCG   >  SRR4302542.1350412/1‑94 (MQ=60)
                                                                                            CTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATGGGGTCCACCGT  >  SRR4302542.504176/1‑94 (MQ=60)
                                                                                            CTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATGGGGTCCACCGT  >  SRR4302542.5808553/1‑94 (MQ=60)
                                                                                            |                                                                                             
TCCGCTGGCGCAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTTTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATGGGGTCCACCGT  >  NC_000913/2824705‑2824890

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑