Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1061 294.1 15065038 98.6% 14854127 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,917,193 A→G V72A (GTG→GCG)  ygcX ← putative glucarate dehydratase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,917,1930AG87.4% 662.9 / 77.9 271V72A (GTG→GCG) ygcXputative glucarate dehydratase
Reads supporting (aligned to +/- strand):  ref base A (19/15);  new base G (138/98);  total (158/113)
Fisher's exact test for biased strand distribution p-value = 8.53e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.23e-01

Only 100 of 285 total aligned reads displayed.

TCTGCAAACCGCGCCCACCAGCATCACGATCGGCAAAAGTATTACGCACCAGCGTCAGAACGTTTTTGTATTCACCCAGCGTTTTACCTACCACCAGCGGAATCGCATCTTCCAGCGTTTTACGGATTTTCTCGCCGCCGGGAATTTCCCCTACGCCAGTGTGACCAGAATTATCTTTGATAATC  >  NC_000913/2917101‑2917285
                                                                                            |                                                                                            
tCTGCAACCCGCGCCCACCAGCATCACGATCGGCAAAAGTATTACGCACCAGCGTCAGAACGTTTTTGTATTCACCCAGCGTTTTACCTacca                                                                                              <  1:1599487/93‑1 (MQ=255)
   gCAAACCGCGCCCACCAGCATCACGATCGGCAAAAGTATTACGCACCAGCGTCAGAACGTTTTTGTATTCACCCAGCGTTTTACCTAccgcca                                                                                           <  1:1115546/93‑1 (MQ=255)
   gCAAACCGCGCCCACCAGCATCACGATCGGCAAAAGTATTACGCACCAGCGTCAGAACGTTTTTGTATTCACCCAGCGTTTTACCTAccgcca                                                                                           <  1:4816916/93‑1 (MQ=255)
     aaaCCGCGCCCACCAGCATCACGATCGGCAAAAGTATTACGCACCAGCGTCAGAACGTTTTTGTATTCACCCAGCGTTTTACCTACCGCCAGc                                                                                         >  1:7246185/1‑93 (MQ=255)
     aaaCCGCGCCCACCAGCATCACGATCGGCAAAAGTATTACGCACCAGCGTCAGAACGTTTTTGTATTCACCCAGCGTTTTACCTACCGCCAGc                                                                                         >  1:278705/1‑93 (MQ=255)
      aaCCGCGCCCACCAGCATCACGATCGGCAAAAGTATTACGCACCAGCGTCAGAACGTTTTTGTATTCACCCAGCGTTTTACCTACCGCCAGCg                                                                                        >  1:4791102/1‑93 (MQ=255)
      aaCCGCGCCCACCAGCATCACGATCGGCAAAAGTATTACGCACCAGCGTCAGAACGTTTTTGTATTCACCCAGCGTTTTACCTACCGCCAGCg                                                                                        >  1:4477279/1‑93 (MQ=255)
       aCCGCGCCCACCAGCATCACGATCGGCAAAAGTATTACGCACCAGCGTCAGAACGTTTTTGTATTCACCCAGCGTTTTACCTACCGCCAGCgg                                                                                       >  1:456115/1‑93 (MQ=255)
       aCCGCGCCCACCAGCATCACGATCGGCAAAAGTATTACGCACCAGCGTCAGAACGTTTTTGTATTCACCCAGCGTTTTACCTACCGCCAGCgg                                                                                       >  2:6613065/1‑93 (MQ=255)
        ccGCGCCCACCAGCATCACGATCGGCAAAAGTATTACGCACCAGCGTCAGAACGTTTTTGTATTCACCCAGCGTTTTACCTACCGCCAGCGGa                                                                                      <  1:798942/93‑1 (MQ=255)
         cgcgCCCACCAGCATCACGATCGGCAAAAGTATTACGCACCAGCGTCAGAACGTTTTTGTATTCACCCAGCGTTTTACCTACCACCAGCGGaa                                                                                     >  1:2853692/1‑93 (MQ=255)
          gcgcCCACCAGCATCACGATCGGCAAAAGTATTACGCACCAGCGTCAGAACGTTTTTGTATTCACCCAGCGTTTTACCTACCGCCAGCGGAAt                                                                                    <  1:375198/93‑1 (MQ=255)
          gcgcCCACCAGCATCACGATCGGCAAAAGTATTACGCACCAGCGTCAGAACGTTTTTGTATTCACCCAGCGTTTTACCTACCGCCAGCGGAAt                                                                                    >  2:4535635/1‑93 (MQ=255)
          gcgcCCACCAGCATCACGATCGGCAAAAGTATTACGCACCAGCGTCAGAACGTTTTTGTATTCACCCAGCGTTTTACCTACCGCCAGCGGAAt                                                                                    <  2:2444697/93‑1 (MQ=255)
           cgcCCACCAGCATCACGATCGGCAAAAGTATTACGCACCAGCGTCAGAACGTTTTTGTATTCACCCAGCGTTTTACCTACCGCCAGCGGAATc                                                                                   <  1:6171308/93‑1 (MQ=255)
           cgcCCACCAGCATCACGATCGGCAAAAGTATTACGCACCAGCGTCAGAACGTTTTTGTATTCACCCAGCGTTTTACCTACCGCCAGCGGAATc                                                                                   <  2:1329440/93‑1 (MQ=255)
            gcCCACCAGCATCACGATCGGCAAAAGTATTACGCACCAGCGTCAGAACGTTTTTGTATTCACCCAGCGTTTTACCTACCGCCAGCGGAATCg                                                                                  >  1:6505334/1‑93 (MQ=255)
             cccACCAGCATCACGATCGGCAAAAGTATTACGCACCAGCGTCAGAACGTTTTTGTATTCACCCAGCGTTTTACCTACCGCCAGCGGAATCGc                                                                                 <  2:3489594/93‑1 (MQ=255)
               caccaGCATCACGATCGGCAAAAGTATTACGCACCAGCGTCAGAACGTTTTTGTATTCACCCAGCGTTTTACCTACCACCAGCGGAATCGCAt                                                                               >  1:1710382/1‑93 (MQ=255)
                  caGCATCACGATCGGCAAAAGTATTACGCACCAGCGTCAGAACGTTTTTGTATTCACCCAGCGTTTTACCTACCGCCAGCGGAATCGCATCtt                                                                            >  2:6523776/1‑93 (MQ=255)
                   aGCATCACGATCGGCAAAAGTATTACGCACCAGCGTCAGAACGTTTTTGTATTCACCCAGCGTTTTACCTACCACCAGCGGAATCGCATCTTc                                                                           <  1:6905755/93‑1 (MQ=255)
                     cATCACGATCGGCAAAAGTATTACGCACCAGCGTCAGAACGTTTTTGTATTCACCCAGCGTTTTACCTACCGCCAGCGGAATCGCATCTTCCa                                                                         <  1:6867528/93‑1 (MQ=255)
                     cATCACGATCGGCAAAAGTATTACGCACCAGCGTCAGAACGTTTTTGTATTCACCCAGCGTTTTACCTACCGCCAGCGGAATCGCATCTTCCa                                                                         >  2:4045101/1‑93 (MQ=255)
                      aTCACGATCGGCAAAAGTATTACGCACCAGCGTCAGAACGTTTTTGTATTCACCCAGCGTTTTACCTACCGCCAGCGGAATCGCATCTTCCAg                                                                        >  2:4648928/1‑93 (MQ=255)
                      aTCACGATCGGCAAAAGTATTACGCACCAGCGTCAGAACGTTTTTGTATTCACCCAGCGTTTTACCTACCGCCAGCGGAATCGCATCTTCCAg                                                                        >  2:6189212/1‑93 (MQ=255)
                       tCACGATCGGCAAAAGTATTACGCACCAGCGTCAGAACGTTTTTGTATTCACCCAGCGTTTTACCTACCGCCAGCGGAATCGCATCTTCCAGc                                                                       >  1:4976221/1‑93 (MQ=255)
                       tCACGATCGGCAAAAGTATTACGCACCAGCGTCAGAACGTTTTTGTATTCACCCAGCGTTTTACCTACCGCCAGCGGAATCGCATCTTCCAGc                                                                       >  2:1547353/1‑93 (MQ=255)
                       tCACGATCGGCAAAAGTATTACGCACCAGCGTCAGAACGTTTTTGTATTCACCCAGCGTTTTACCTACCGCCAGCGGAATCGCATCTTCCAGc                                                                       >  2:5747212/1‑93 (MQ=255)
                       tCACGATCGGCAAAAGTATTACGCACCAGCGTCAGAACGTTTTTGTATTCACCCAGCGTTTTACCTACCACCAGCGGAATCGCATCTTCCAGc                                                                       >  1:3259554/1‑93 (MQ=255)
                        cACGATCGGCAAAAGTATTACGCACCAGCGTCAGAACGTTTTTGTATTCACCCAGCGTTTTACCTACCGCCAGCGGAATCGCATCTTCCAGCg                                                                      >  1:6991433/1‑93 (MQ=255)
                          cGATCGGCAAAAGTATTACGCACCAGCGTCAGAACGTTTTTGTATTCACCCAGCGTTTTACCTACCGCCAGCGGAATCGCATCTTCCAGCGtt                                                                    >  2:2074876/1‑93 (MQ=255)
                             tCGGCAAAAGTATTACGCACCAGCGTCAGAACGTTTTTGTATTCACCCAGCGTTTTACCTACCGCCAGCGGAATCGCATCTTCCAGCGTTTTa                                                                 >  1:3882401/1‑93 (MQ=255)
                             tCGGCAAAAGTATTACGCACCAGCGTCAGAACGTTTTTGTATTCACCCAGCGTTTTACCTACCGCCAGCGGAATCGCATCTTCCAGCGTTTTa                                                                 <  2:247801/93‑1 (MQ=255)
                              cGGCAAAAGTATTACGCACCAGCGTCAGAACGTTTTTGTATTCACCCAGCGTTTTACCTACCGCCAGCGGAATCGCATCTTCCAGCGTTTTAc                                                                <  2:6045947/93‑1 (MQ=255)
                               ggCAAAAGTATTACGCACCAGCGTCAGAACGTTTTTGTATTCACCCAGCGTTTTACCTACCGCCAGCGGAATCGCATCTTCCAGCGTTTTACg                                                               >  2:3710039/1‑93 (MQ=255)
                               ggCAAAAGTATTACGCACCAGCGTCAGAACGTTTTTGTATTCACCCAGCGTTTTACCTACCGCCAGCGGAATCGCATCTTCCAGCGTTTTACg                                                               >  2:305565/1‑93 (MQ=255)
                               ggCAAAAGTATTACGCACCAGCGTCAGAACGTTTTTGTATTCACCCAGCGTTTTACCTACCGCCAGCGGAATCGCATCTTCCAGCGTTTTACg                                                               >  1:6716927/1‑93 (MQ=255)
                                 cAAAAGTATTACGCACCAGCGTCAGAACGTTTTTGTATTCACCCAGCGTTTTACCTACCGCCAGCGGAATCGCATCTTCCAGCGTTTTACGGa                                                             >  2:5459063/1‑93 (MQ=255)
                                 cAAAAGTATTACGCACCAGCGTCAGAACGTTTTTGTATTCACCCAGCGTTTTACCTACCGCCAGCGGAATCGCATCTTCCAGCGTTTTACGGa                                                             <  2:7107087/93‑1 (MQ=255)
                                 cAAAAGTATTACGCACCAGCGTCAGAACGTTTTTGTATTCACCCAGCGTTTTACCTACCGCCAGCGGAATCGCATCTTCCAGCGTTTTACGGa                                                             <  2:5200990/93‑1 (MQ=255)
                                 cAAAAGTATTACGCACCAGCGTCAGAACGTTTTTGTATTCACCCAGCGTTTTACCTACCACCAGCGGAATCGCATCTTCCAGCGTTTTACGGa                                                             >  2:2595210/1‑93 (MQ=255)
                                  aaaaGTATTACGCACCAGCGTCAGAACGTTTTTGTATTCACCCAGCGTTTTACCTACCACCAGCGGAATCGCATCTTCCAGCGTTTTACGGAt                                                            <  1:5256426/93‑1 (MQ=255)
                                   aaaGTATTACGCACCAGCGTCAGAACGTTTTTGTATTCACCCAGCGTTTTACCTACCGCCAGCGGAATCGCATCTTCCAGCGTTTTACGGAtt                                                           >  1:6311922/1‑93 (MQ=255)
                                   aaaGTATTACGCACCAGCGTCAGAACGTTTTTGTATTCACCCAGCGTTTTACCTACCGCCAGCGGAATCGCATCTTCCAGCGTTTTACGGAtt                                                           >  1:1485894/1‑93 (MQ=255)
                                   aaaGTATTACGCACCAGCGTCAGAACGTTTTTGTATTCACCCAGCGTTTTACCTACCGCCAGCGGAATCGCATCTTCCAGCGTTTTACGGAtt                                                           >  2:2332134/1‑93 (MQ=255)
                                    aaGTATTACGCACCAGCGTCAGAACGTTTTTGTATTCACCCAGCGTTTTACCTACCGCCAGCGGAATCGCATCTTCCAGCGTTTTACGGAttt                                                          >  1:5990875/1‑93 (MQ=255)
                                     aGTATTACGCACCAGCGTCAGAACGTTTTTGTATTCACCCAGCGTTTTACCTACCGCCAGCGGAATCGCATCTTCCAGCGTTTTACGGAtttt                                                         <  1:5663235/93‑1 (MQ=255)
                                       tATTACGCACCAGCGTCAGAACGTTTTTGTATTCACCCAGCGTTTTACCTACCGCCAGCGGAATCGCATCTTCCAGCGTTTTACGGATTTtct                                                       >  1:1087078/1‑93 (MQ=255)
                                        aTTACGCACCAGCGTCAGAACGTTTTTGTATTCACCCAGCGTTTTACCTACCGCCAGCGGAATCGCATCTTCCAGCGTTTTACGGATTTtctc                                                      <  1:511160/93‑1 (MQ=255)
                                        aTTACGCACCAGCGTCAGAACGTTTTTGTATTCACCCAGCGTTTTACCTACCACCAGCGGAATCGCATCTTCCAGCGTTTTACGGATTTtctc                                                      >  1:3164212/1‑93 (MQ=255)
                                        aTTACGCACCAGCGTCAGAACGTTTTTGTATTCACCCAGCGTTTTACCTACCACCAGCGGAATCGCATCTTCCAGCGTTTTACGGATTTtctc                                                      >  1:352227/1‑93 (MQ=255)
                                             gCACCAGCGTCAGAACGTTTTTGTATTCACCCAGCGTTTTACCTACCGCCAGCGGAATCGCATCTTCCAGCGTTTTACGGATTTTCTcgccgc                                                 >  2:1033187/1‑93 (MQ=255)
                                             gCACCAGCGTCAGAACGTTTTTGTATTCACCCAGCGTTTTACCTACCGCCAGCGGAATCGCATCTTCCAGCGTTTTACGGATTTTCTcgccgc                                                 <  1:729510/93‑1 (MQ=255)
                                             gCACCAGCGTCAGAACGTTTTTGTATTCACCCAGCGTTTTACCTACCGCCAGCGGAATCGCATCTTCCAGCGTTTTACGGATTTTCTcgccgc                                                 >  2:1708699/1‑93 (MQ=255)
                                                ccAGCGTCAGAACGTTTTTGTATTCACCCAGCGTTTTACCTACCGCCAGCGGAATCGCATCTTCCAGCGTTTTACGGATTTTCTCGCCGCCgg                                              >  1:463613/1‑93 (MQ=255)
                                                ccAGCGTCAGAACGTTTTTGTATTCACCCAGCGTTTTACCTACCACCAGCGGAATCGCATCTTCCAGCGTTTTACGGATTTTCTCGCCGCCgg                                              >  2:4126819/1‑93 (MQ=255)
                                                   gCGTCAGAACGTTTTTGTATTCACCCAGCGTTTTACCTACCGCCAGCGGAATCGCATCTTCCAGCGTTTTACGGATTTTCTCGCCGCCGGGaa                                           <  1:1067353/93‑1 (MQ=255)
                                                     gTCAGAACGTTTTTGTATTCACCCAGCGTTTTACCTACCGCCAGCGGAATCGCATCTTCCAGCGTTTTACGGATTTTCTCGCCGCCGGGAAtt                                         >  1:6580500/1‑93 (MQ=255)
                                                      tCAGAACGTTTTTGTATTCACCCAGCGTTTTACCTACCGCCAGCGGAATCGCATCTTCCAGCGTTTTACGGATTTTCTCGCCGCCGGGAAttt                                        >  2:7133849/1‑93 (MQ=255)
                                                        aGAACGTTTTTGTATTCACCCAGCGTTTTACCTACCGCCAGCGGAATCGCATCTTCCAGCGTTTTACGGATTTTCTCGCCGCCGGGAATTTcc                                      >  1:403954/1‑93 (MQ=255)
                                                        aGAACGTTTTTGTATTCACCCAGCGTTTTACCTACCACCAGCGGAATCGCATCTTCCAGCGTTTTACGGATTTTCTCGCCGCCGGGAATTTcc                                      <  2:2784834/93‑1 (MQ=255)
                                                         gAACGTTTTTGTATTCACCCAGCGTTTTACCTACCGCCAGCGGAATCGCATCTTCCAGCGTTTTACGGATTTTCTCGCCGCCGGGAATTTccc                                     <  1:4693863/93‑1 (MQ=255)
                                                         aacggTTTTTTTTTTCCCCCAGGGTTTTCCCTCCCCCCAGGGGAATCCCATTTTCCAGGGTTTTACGGTTTTTCTCCCCGCCGGGAATTTccc                                     >  1:2366546/5‑93 (MQ=255)
                                                           aCGTTTTTGTATTCACCCAGCGTTTTACCTACCGCCAGCGGAATCGCATCTTCCAGCGTTTTACGGATTTTCTCGCCGCCGGGAATTTCCCCt                                   >  2:7322059/1‑93 (MQ=255)
                                                           aCGTTTTTGTATTCACCCAGCGTTTTACCTACCGCCAGCGGAATCGCATCTTCCAGCGTTTTACGGATTTTCTCGCCGCCGGGAATTTCCCCt                                   >  1:10150/1‑93 (MQ=255)
                                                             gTTTTTGTATTCACCCAGCGTTTTACCTACCGCCAGCGGAATCGCATCTTCCAGCGTTTTACGGATTTTCTCGCCGCCGGGAATTTCCCCTAc                                 <  2:4799600/93‑1 (MQ=255)
                                                             gTTTTTGTATTCACCCAGCGTTTTACCTACCGCCAGCGGAATCGCATCTTCCAGCGTTTTACGGATTTTCTCGCCGCCGGGAATTTCCCCTAc                                 <  2:2720637/93‑1 (MQ=255)
                                                             gTTTTTGTATTCACCCAGCGTTTTACCTACCGCCAGCGGAATCGCATCTTCCAGCGTTTTACGGATTTTCTCGCCGCCGGGAATTTCCCCTAc                                 <  1:1401498/93‑1 (MQ=255)
                                                               ttttGTATTCACCCAGCGTTTTACCTACCGCCAGCGGAATCGCATCTTCCAGCGTTTTACGGATTTTCTCGCCGCCGGGAATTTCCCCTACGc                               >  2:4406495/1‑93 (MQ=255)
                                                                 ttGTATTCACCCAGCGTTTTACCTACCGCCAGCGGAATCGCATCTTCCAGCGTTTTACGGATTTTCTCGCCGCCGGGAATTTCCCCTACGCCa                             <  1:4308558/93‑1 (MQ=255)
                                                                  tGTATTCACCCAGCGTTTTACCTACCGCCAGCGGAATCGCATCTTCCAGCGTTTTACGGATTTTCTCGCCGCCGGGAATTTCCCCTACGCCAg                            <  2:881264/93‑1 (MQ=255)
                                                                   gTATTCACCCAGCGTTTTACCTACCGCCAGCGGAATCGCATCTTCCAGCGTTTTACGGATTTTCTCGCCGCCGGGAATTTCCCCTACGCCAgt                           >  1:907465/1‑93 (MQ=255)
                                                                   gTATTCACCCAGCGTTTTACCTACCACCAGCGGAATCGCATCTTCCAGCGTTTTACGGATTTTCTCGCCGCCGGGAATTTCCCCTACGCCAgt                           <  2:6000053/93‑1 (MQ=255)
                                                                    tATTCACCCAGCGTTTTACCTACCGCCAGCGGAATCGCATCTTCCAGCGTTTTACGGATTTTCTCGCCGCCGGGAATTTCCCCTACGCCAgtg                          >  1:1283820/1‑93 (MQ=255)
                                                                     aTTCACCCAGCGTTTTACCTACCGCCAGCGGAATCGCATCTTCCAGCGTTTTACGGATTTTCTCGCCGCCGGGAATTTCCCCTACGCCAgtgt                         >  1:223281/1‑93 (MQ=255)
                                                                     aTTCACCCAGCGTTTTACCTACCGCCAGCGGAATCGCATCTTCCAGCGTTTTACGGATTTTCTCGCCGCCGGGAATTTCCCCTACGCCAgtgt                         <  1:194779/93‑1 (MQ=255)
                                                                        cACCCAGCGTTTTACCTACCGCCAGCGGAATCGCATCTTCCAGCGTTTTACGGATTTTCTCGCCGCCGGGAATTTCCCCTACGCCAGTGTGAc                      >  2:3014606/1‑93 (MQ=255)
                                                                          cccAGCGTTTTACCTACCGCCAGCGGAATCGCATCTTCCAGCGTTTTACGGATTTTCTCGCCGCCGGGAATTTCCCCTACGCCAGTGTGACCa                    >  2:7411282/1‑93 (MQ=255)
                                                                          cccAGCGTTTTACCTACCGCCAGCGGAATCGCATCTTCCAGCGTTTTACGGATTTTCTCGCCGCCGGGAATTTCCCCTACGCCAGTGTGACCa                    >  2:5294942/1‑93 (MQ=255)
                                                                          cccAGCGTTTTACCTACCGCCAGCGGAATCGCATCTTCCAGCGTTTTACGGATTTTCTCGCCGCCGGGAATTTCCCCTACGCCAGTGTGACCa                    >  2:1653597/1‑93 (MQ=255)
                                                                           ccAGCGTTTTACCTACCGCCAGCGGAATCGCATCTTCCAGCGTTTTACGGATTTTCTCGCCGCCGGGAATTTCCCCTACGCCAGTGTGACCAg                   >  1:548343/1‑93 (MQ=255)
                                                                             aGCGTTTTACCTACCGCCAGCGGAATCGCATCTTCCAGCGTTTTACGGATTTTCTCGCCGCCGGGAATTTCCCCTACGCCAGTGTGACCAGaa                 <  2:1205990/93‑1 (MQ=255)
                                                                             aGCGTTTTACCTACCGCCAGCGGAATCGCATCTTCCAGCGTTTTACGGATTTTCTCGCCGCCGGGAATTTCCCCTACGCCAGTGTGACCAGaa                 <  2:3332329/93‑1 (MQ=255)
                                                                             aGCGTTTTACCTACCACCAGCGGAATCGCATCTTCCAGCGTTTTACGGATTTTCTCGCCGCCGGGAATTTCCCCTACGCCAGTGTGACCAGaa                 >  2:6309022/1‑93 (MQ=255)
                                                                              gCGTTTTACCTACCGCCAGCGGAATCGCATCTTCCAGCGTTTTACGGATTTTCTCGCCGCCGGGAATTTCCCCTACGCCAGTGTGACCAGAAt                <  2:1962990/93‑1 (MQ=255)
                                                                              gCGTTTTACCTACCGCCAGCGGAATCGCATCTTCCAGCGTTTTACGGATTTTCTCGCCGCCGGGAATTTCCCCTACGCCAGTGTGACCAGAAt                <  2:80962/93‑1 (MQ=255)
                                                                              gCGTTTTACCTACCGCCAGCGGAATCGCATCTTCCAGCGTTTTACGGATTTTCTCGCCGCCGGGAATTTCCCCTACGCCAGTGTGACCAGAAt                <  1:5532551/93‑1 (MQ=255)
                                                                              gCGTTTTACCTACCGCCAGCGGAATCGCATCTTCCAGCGTTTTACGGATTTTCTCGCCGCCGGGAATTTCCCCTACGCCAGTGTGACCAGAAt                >  2:3217015/1‑93 (MQ=255)
                                                                                gTTTTACCTCCCGCCAGCGGAATCGCATCTTCCAGCGTTTTACGGATTTTCTCGCCGCCGGGAATTT‑CCCTACGCCAGTGTGACCAGAATTAt             <  2:3775722/93‑1 (MQ=255)
                                                                                gTTTTACCTACCGCCAGCGGAATCGCATCTTCCAGCGTTTTACGGATTTTCTCGCCGCCGGGAATTTCCCCTACGCCAGTGTGACCAGAATTa              <  1:2908683/93‑1 (MQ=255)
                                                                                gTTTTACCTACCACCAGCGGAATCGCATCTTCCAGCGTTTTACGGATTTTCTCGCCGCCGGGAATTTCCCCTACGCCAGTGTGACCAGAATTa              <  2:4242478/93‑1 (MQ=255)
                                                                                 ttttACCTACCGCCAGCGGAATCGCATCTTCCAGCGTTTTACGGATTTTCTCGCCGCCGGGAATTTCCCCTACGCCAGTGTGACCAGAATTAt             >  2:5113671/1‑93 (MQ=255)
                                                                                 ttttACCTACCGCCAGCGGAATCGCATCTTCCAGCGTTTTACGGATTTTCTCGCCGCCGGGAATTTCCCCTACGCCAGTGTGACCAGAATTAt             >  1:5792094/1‑93 (MQ=255)
                                                                                   ttACCTACCGCCAGCGGAATCGCATCTTCCAGCGTTTTACGGATTTTCTCGCCGCCGGGAATTTCCCCTACGCCAGTGTGACCAGAATTATCt           <  2:6986004/93‑1 (MQ=255)
                                                                                   tcacctaccGCCAGCGGAATCGCATCTTCCAGCGTTTTACGGATTTTCTCGCCGCCGGGAATTTCCCCTACGCCAGTGTGACCAGAATTATCt           <  2:6442426/91‑1 (MQ=255)
                                                                                     acctaccGCCAGCGGAATCGCATCTTCCAGCGTTTTACGGATTTTCTCGCCGCCGGGAATTTCCCCTACGCCAGTGTGACCAGAATTATCttt         <  2:3434861/93‑1 (MQ=255)
                                                                                      cctaccGCCAGCGGAATCGCATCTTCCAGCGTTTTACGGATTTTCTCGCCGCCGGGAATTTCCCCTACGCCAGTGTGACCAGAATTATCTTTg        <  2:5628141/93‑1 (MQ=255)
                                                                                      cctaccGCCAGCGGAATCGCATCTTCCAGCGTTTTACGGATTTTCTCGCCGCCGGGAATTTCCCCTACGCCAGTGTGACCAGAATTATCTTTg        <  1:3352622/93‑1 (MQ=255)
                                                                                       ctaccGCCAGCGGAATCGCATCTTCCAGCGTTTTACGGATTTTCTCGCCGCCGGGAATTTCCCCTACGCCAGTGTGACCAGAATTATCTTTGa       <  1:3016510/93‑1 (MQ=255)
                                                                                           caccaGCGGAATCGCATCTTCCAGCGTTTTACGGATTTTCTCGCCGCCGGGAATTTCCCCTACGCCAGTGTGACCAGAATTATCTTTGATAAt   <  2:2953459/93‑1 (MQ=255)
                                                                                            |                                                                                            
TCTGCAAACCGCGCCCACCAGCATCACGATCGGCAAAAGTATTACGCACCAGCGTCAGAACGTTTTTGTATTCACCCAGCGTTTTACCTACCACCAGCGGAATCGCATCTTCCAGCGTTTTACGGATTTTCTCGCCGCCGGGAATTTCCCCTACGCCAGTGTGACCAGAATTATCTTTGATAATC  >  NC_000913/2917101‑2917285

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GTCTGCAAACCGCGCCCACCAGCATCACGATCGGCAAAAGTATTACGCACCAGCGTCAGAACGTTTTTGTATTCACCCAGCGTTTTACCTACCACCAGCGGAATCGCATCTTCCAGCGTTTTACGGATTTTCTCGCCGCCGGGAATTTCCCCTACGCCAGTGTGACCAGAATTATCTTTGATAATC  >  NC_000913/2917100‑2917285
                                                                                             |                                                                                            
GTCTGCAAACCGCGCCCACCAGCATCACGATCGGCAAAAGTATTACGCACCAGCGTCAGAACGTTTTTGTATTCACCCAGCGTTTTACCTACCG                                                                                              >  SRR4302542.4519650/1‑94 (MQ=60)
GTCTGCAAACCGCGCCCACCAGCATCACGATCGGCAAAAGTATTACGCACCAGCGTCAGAACGTTTTTGTATTCACCCAGCGTTTTACCTACCG                                                                                              >  SRR4302542.7181123/1‑94 (MQ=60)
GTCTGCAAACCGCGCCCACCAGCATCACGATCGGCAAAAGTATTACGCACCAGCGTCAGAACGTTTTTGTATTCACCCAGCGTTTTACCTACCG                                                                                              <  SRR4302542.3203061/94‑1 (MQ=60)
 TCTGCAACCCGCGCCCACCAGCATCACGATCGGCAAAAGTATTACGCACCAGCGTCAGAACGTTTTTGTATTCACCCAGCGTTTTACCTACCAC                                                                                             <  SRR4302542.1613303/94‑1 (MQ=60)
   tccaatccttgcctcgtgGCATCACGATCAGCAAAAGTATTACCCTCCAGCGTCAGAACGTGTTTGTATTCACCCAGCGTTTTGCCTACCGCCA                                                                                           <  SRR4302542.4878323/76‑1 (MQ=59)
   TGCAAACCGCGCCCACCAGCATCACGATCGGCAAAAGTATTACGCACCAGCGTCAGAACGTTTTTGTATTCACCCAGCGTTTTACCTACCGCCA                                                                                           <  SRR4302542.6749289/94‑1 (MQ=60)
    GCAAACCGCGCCCACCAGCATCACGATCGGCAAAAGTATTACGCACCAGCGTCAGAACGTTTTTGTATTCACCCAGCGTTTTACCTACCGCCAG                                                                                          <  SRR4302542.3039295/94‑1 (MQ=60)
    GCAAACCGCGCCCACCAGCATCACGATCGGCAAAAGTATTACGCACCAGCGTCAGAACGTTTTTGTATTCACCCAGCGTTTTACCTACCGCCAG                                                                                          <  SRR4302542.1125077/94‑1 (MQ=60)
    GCAAACCGCGCCCACCAGCATCACGATCGGCAAAAGTATTACGCACCAGCGTCAGAACGTTTTTGTATTCACCCAGCGTTTTACCTACCGCCAG                                                                                          <  SRR4302542.1308391/94‑1 (MQ=60)
    GCAAACCGCGCCCACCAGCATCACGATCGGCAAAAGTATTACGCACCAGCGTCAGAACGTTTTTGTATTCACCCAGCGTTTTACCTACCGCCAG                                                                                          <  SRR4302542.4863858/94‑1 (MQ=60)
     CAAACCGCGCCCACCAGCATCACGATCGGCAAAAGTATTACGCACCAGCGTCAGAACGTTTTTGTATTCACCCAGCGTTTTACCTACCGCCAGC                                                                                         >  SRR4302542.281088/1‑94 (MQ=60)
     CAAACCGCGCCCACCAGCATCACGATCGGCAAAAGTATTACGCACCAGCGTCAGAACGTTTTTGTATTCACCCAGCGTTTTACCTACCGCCAGC                                                                                         >  SRR4302542.1092342/1‑94 (MQ=60)
     CAAACCGCGCCCACCAGCATCACGATCGGCAAAAGTATTACGCACCAGCGTCAGAACGTTTTTGTATTCACCCAGCGTTTTACCTACCGCCAGC                                                                                         >  SRR4302542.7317243/1‑94 (MQ=60)
     CAAACCGCGCCCACCAGCATCACGATCGGCAAAAGTATTACGCACCAGCGTCAGAACGTTTTTGTATTCACCCAGCGTTTTACCTACCACCAGC                                                                                         >  SRR4302542.5141636/1‑94 (MQ=60)
      AAACCGCGCCCACCAGCATCACGATCGGCAAAAGTATTACGCACCAGCGTCAGAACGTTTTTGTATTCACCCAGCGTTTTACCTACCGCCAGCG                                                                                        >  SRR4302542.4837789/1‑94 (MQ=60)
      AAACCGCGCCCACCAGCATCACGATCGGCAAAAGTATTACGCACCAGCGTCAGAACGTTTTTGTATTCACCCAGCGTTTTACCTACCGCCAGCG                                                                                        >  SRR4302542.4521189/1‑94 (MQ=60)
       AACCGCGCCCACCAGCATCACGATCGGCAAAAGTATTACGCACCAGCGTCAGAACGTTTTTGTATTCACCCAGCGTTTTACCTACCGCCAGCGG                                                                                       >  SRR4302542.460058/1‑94 (MQ=60)
        ACCGCGCCCACCAGCATCACGATCGGCAAAAGTATTACGCACCAGCGTCAGAACGTTTTTGTATTCACCCAGCGTTTTACCTACCGCCAGCGGA                                                                                      >  SRR4302542.411803/1‑94 (MQ=60)
         CCGCGCCCACCAGCATCACGATCGGCAAAAGTATTACGCACCAGCGTCAGAACGTTTTTGTATTCACCCAGCGTTTTACCTACCGCCAGCGGAA                                                                                     <  SRR4302542.805809/94‑1 (MQ=60)
         CCGCGCCCACCAGCATCACGATCGGCAAAAGTATTACGCACCAGCGTCAGAACGTTTTTGTATTCACCCAGCGTTTTACCTACCACCAGCGGAA                                                                                     >  SRR4302542.2881114/1‑94 (MQ=60)
          CGCGCCCACCAGCATCACGATCGGCAAAAGTATTACGCACCAGCGTCAGAACGTTTTTGTATTCACCCAGCGTTTTACCTACCGCCAGCGGAAT                                                                                    >  SRR4302542.4860430/1‑94 (MQ=60)
          CGCGCCCACCAGCATCACGATCGGCAAAAGTATTACGCACCAGCGTCAGAACGTTTTTGTATTCACCCAGCGTTTTACCTACCGCCAGCGGAAT                                                                                    >  SRR4302542.3220901/1‑94 (MQ=60)
          CGCGCCCACCAGCATCACGATCGGCAAAAGTATTACGCACCAGCGTCAGAACGTTTTTGTATTCACCCAGCGTTTTACCTACCGCCAGCGGAAT                                                                                    >  SRR4302542.482353/1‑94 (MQ=60)
           GCGCCCACCAGCATCACGATCGGCAAAAGTATTACGCACCAGCGTCAGAACGTTTTTGTATTCACCCAGCGTTTTACCTACCGCCAGCGGAATC                                                                                   <  SRR4302542.378464/94‑1 (MQ=60)
           GCGCCCACCAGCATCACGATCGGCAAAAGTATTACGCACCAGCGTCAGAACGTTTTTGTATTCACCCAGCGTTTTACCTACCACCAGCGGAATC                                                                                   <  SRR4302542.4965964/94‑1 (MQ=60)
            CGCCCACCAGCATCACGATCGGCAAAAGTATTACGCACCAGCGTCAGAACGTTTTTGTATTCACCCAGCGTTTTACCTACCGCCAGCGGAATCG                                                                                  <  SRR4302542.6231115/94‑1 (MQ=60)
            CGCCCACCAGCATCACGATCGGCAAAAGTATTACGCACCAGCGTCAGAACGTTTTTGTATTCACCCAGCGTTTTACCTACCGCCAGCGGAATCG                                                                                  >  SRR4302542.6569464/1‑94 (MQ=60)
             GCCCACCAGCATCACGATCGGCAAAAGTATTACGCACCAGCGTCAGAACGTTTTTGTATTCACCCAGCGTTTTACCTACCGCCAGCGGAATCGC                                                                                 >  SRR4302542.7338339/1‑94 (MQ=60)
              CCCACCAGCATCACGATCGGCAAAAGTATTACGCACCAGCGTCAGAACGTTTTTGTATTCACCCAGCGTTTTACCTACCGCCAGCGGAATCGCA                                                                                >  SRR4302542.638646/1‑94 (MQ=60)
               CCACCAGCATCACGATCGGCAAAAGTATTACGCACCAGCGTCAGAACGTTTTTGTATTCACCCAGCGTTTTACCTACCGCCAGCGGAATCGCAT                                                                               >  SRR4302542.955037/1‑94 (MQ=60)
               CCACCAGCATCACGATCGGCAAAAGTATTACGCACCAGCGTCAGAACGTTTTTGTATTCACCCAGCGTTTTACCTACCGCCAGCGGAATCGCAT                                                                               <  SRR4302542.4628329/94‑1 (MQ=60)
               CCACCAGCATCACGATCGGCAAAAGTATTACGCACCAGCGTCAGAACGTTTTTGTATTCACCCAGCGTTTTACCTACCACCAGCGGAATCGCAT                                                                               >  SRR4302542.1725242/1‑94 (MQ=60)
                    AGCATCACGATCGGCAAAAGTATTACGCACCAGCGTCAGAACGTTTTTGTATTCACCCAGCGTTTTACCTACCGCCAGCGGAATCGCATCTTCC                                                                          >  SRR4302542.4821965/1‑94 (MQ=60)
                    AGCATCACGATCGGCAAAAGTATTACGCACCAGCGTCAGAACGTTTTTGTATTCACCCAGCGTTTTACCTACCGCCAGCGGAATCGCATCTTCC                                                                          >  SRR4302542.6765246/1‑94 (MQ=60)
                    AGCATCACGATCGGCAAAAGTATTACGCACCAGCGTCAGAACGTTTTTGTATTCACCCAGCGTTTTACCTACCACCAGCGGAATCGCATCTTCC                                                                          <  SRR4302542.6973552/94‑1 (MQ=60)
                     GCATCACGATCGGCAAAAGTATTACGCACCAGCGTCAGAACGTTTTTGTATTCACCCAGCGTTTTACCTACCACCAGCGGAATCGCATCTTCCA                                                                         <  SRR4302542.1883376/94‑1 (MQ=60)
                      CATCACGATCGGCAAAAGTATTACGCACCAGCGTCAGAACGTTTTTGTATTCACCCAGCGTTTTACCTACCGCCAGCGGAATCGCATCTTCCAG                                                                        <  SRR4302542.6934990/94‑1 (MQ=60)
                       ATCACGATCGGCAAAAGTATTACGCACCAGCGTCAGAACGTTTTTGTATTCACCCAGCGTTTTACCTACCGCCAGCGGAATCGCATCTTCCAGC                                                                       >  SRR4302542.6484506/1‑94 (MQ=60)
                       ATCACGATCGGCAAAAGTATTACGCACCAGCGTCAGAACGTTTTTGTATTCACCCAGCGTTTTACCTACCGCCAGCGGAATCGCATCTTCCAGC                                                                       >  SRR4302542.5024533/1‑94 (MQ=60)
                       ATCACGATCGGCAAAAGTATTACGCACCAGCGTCAGAACGTTTTTGTATTCACCCAGCGTTTTACCTACCACCAGCGGAATCGCATCTTCCAGC                                                                       >  SRR4302542.3290337/1‑94 (MQ=60)
                        TCACGATCGGCAAAAGTATTACGCACCAGCGTCAGAACGTTTTTGTATTCACCCAGCGTTTTACCTACCGCCAGCGGAATCGCATCTTCCAGCG                                                                      >  SRR4302542.7060115/1‑94 (MQ=60)
                        TCACGATCGGCAAAAGTATTACGCACCAGCGTCAGAACGTTTTTGTATTCACCCAGCGTTTTACCTACCGCCAGCGGAATCGCATCTTCCAGCG                                                                      <  SRR4302542.5150889/94‑1 (MQ=60)
                        TCACGATCGGCAAAAGTATTACGCACCAGCGTCAGAACGTTTTTGTATTCACCCAGCGTTTTACCTACCACCAGCGGAATCGCATCTTCCAGCG                                                                      <  SRR4302542.6273629/94‑1 (MQ=60)
                         CACGATCGGCAAAAGTATTACGCACCAGCGTCAGAACGTTTTTGTATTCACCCAGCGTTTTACCTACCGCCAGCGGAATCGCATCTTCCAGCGT                                                                     >  SRR4302542.562882/1‑94 (MQ=60)
                         CACGATCGGCAAAAGTATTACGCACCAGCGTCAGAACGTTTTTGTATTCACCCAGCGTTTTACCTACCACCAGCGGAATCGCATCTTCCAGCGT                                                                     <  SRR4302542.6343346/94‑1 (MQ=60)
                         CACGATCGGCAAAAGTATTACGCACCAGCGTCAGAACGTTTTTGTATTCACCCAGCGTTTTACCTACCACCAGCGGAATCGCATCTTCCAGCGT                                                                     >  SRR4302542.1434212/1‑94 (MQ=60)
                           CGATCGGCAAAAGTATTACGCACCAGCGTCAGAACGTTTTTGTATTCACCCAGCGTTTTACCTACCGCCAGCGGAATCGCATCTTCCAGCGTTT                                                                   >  SRR4302542.7027349/1‑94 (MQ=60)
                            GATCGGCAAAAGTATTACGCACCAGCGTCAGAACGTTTTTGTATTCACCCAGCGTTTTACCTACCACCAGCGGAATCGCATCTTCCAGCGTTTT                                                                  >  SRR4302542.4525101/1‑94 (MQ=60)
                             ATCGGCAAAAGTATTACGCACCAGCGTCAGAACGTTTTTGTATTCACCCAGCGTTTTACCTACCGCCAGCGGAATCGCATCTTCCAGCGTTTTA                                                                 >  SRR4302542.3920744/1‑94 (MQ=60)
                               CGGCAAAAGTATTACGCACCAGCGTCAGAACGTTTTTGTATTCACCCAGCGTTTTACCTACCGCCAGCGGAATCGCATCTTCCAGCGTTTTACG                                                               >  SRR4302542.6964906/1‑94 (MQ=60)
                               CGGCAAAAGTATTACGCACCAGCGTCAGAACGTTTTTGTATTCACCCAGCGTTTTACCTACCGCCAGCGGAATCGCATCTTCCAGCGTTTTACG                                                               >  SRR4302542.6783083/1‑94 (MQ=60)
                                  CAAAAGTATTACGCACCAGCGTCAGAACGTTTTTGTATTCACCCAGCGTTTTACCTACCGCCAGCGGAATCGCATCTTCCAGCGTTTTACGGAT                                                            >  SRR4302542.1100473/1‑94 (MQ=60)
                                   AAAAGTATTACGCACCAGCGTCAGAACGTTTTTGTATTCACCCAGCGTTTTACCTACCGCCAGCGGAATCGCATCTTCCAGCGTTTTACGGATT                                                           >  SRR4302542.5798414/1‑94 (MQ=60)
                                   AAAAGTATTACGCACCAGCGTCAGAACGTTTTTGTATTCACCCAGCGTTTTACCTACCGCCAGCGGAATCGCATCTTCCAGCGTTTTACGGATT                                                           >  SRR4302542.1498723/1‑94 (MQ=60)
                                   AAAAGTATTACGCACCAGCGTCAGAACGTTTTTGTATTCACCCAGCGTTTTACCTACCGCCAGCGGAATCGCATCTTCCAGCGTTTTACGGATT                                                           >  SRR4302542.5369219/1‑94 (MQ=60)
                                   AAAAGTATTACGCACCAGCGTCAGAACGTTTTTGTATTCACCCAGCGTTTTACCTACCGCCAGCGGAATCGCATCTTCCAGCGTTTTACGGATT                                                           <  SRR4302542.1678391/94‑1 (MQ=60)
                                   AAAAGTATTACGCACCAGCGTCAGAACGTTTTTGTATTCACCCAGCGTTTTACCTACCGCCAGCGGAATCGCATCTTCCAGCGTTTTACGGATT                                                           >  SRR4302542.6374112/1‑94 (MQ=60)
                                   AAAAGTATTACGCACCAGCGTCAGAACGTTTTTGTATTCACCCAGCGTTTTACCTACCACCAGCGGAATCGCATCTTCCAGCGTTTTACGGATT                                                           <  SRR4302542.5307514/94‑1 (MQ=60)
                                    AAAGTATTACGCACCAGCGTCAGAACGTTTTTGTATTCACCCAGCGTTTTACCTACCGCCAGCGGAATCGCATCTTCCAGCGTTTTACGGATTT                                                          >  SRR4302542.331430/1‑94 (MQ=60)
                                    AAAGTATTACGCACCAGCGTCAGAACGTTTTTGTATTCACCCAGCGTTTTACCTACCGCCAGCGGAATCGCATCTTCCAGCGTTTTACGGATTT                                                          >  SRR4302542.751844/1‑94 (MQ=60)
                                    AAAGTATTACGCACCAGCGTCAGAACGTTTTTGTATTCACCCAGCGTTTTACCTACCGCCAGCGGAATCGCATCTTCCAGCGTTTTACGGATTT                                                          >  SRR4302542.6048903/1‑94 (MQ=60)
                                     AAGTATTACGCACCAGCGTCAGAACGTTTTTGTATTCACCCAGCGTTTTACCTACCGCCAGCGGAATCGCATCTTCCAGCGTTTTACGGATTTT                                                         >  SRR4302542.1055330/1‑94 (MQ=60)
                                      AGTATTACGCACCAGCGTCAGAACGTTTTTGTATTCACCCAGCGTTTTACCTACCGCCAGCGGAATCGCATCTTCCAGCGTTTTACGGATTTTC                                                        <  SRR4302542.5718244/94‑1 (MQ=60)
                                      AGTATTACGCACCAGCGTCAGAACGTTTTTGTATTCACCCAGCGTTTTACCTACCGCCAGCGGAATCGCATCTTCCAGCGTTTTACGGATTTTC                                                        >  SRR4302542.6106382/1‑94 (MQ=60)
                                       GTATTACGCACCAGCGTCAGAACGTTTTTGTATTCACCCAGCGTTTTACCTACCGCCAGCGGAATCGCATCTTCCAGCGTTTTACGGATTTTCT                                                       >  SRR4302542.1096399/1‑94 (MQ=60)
                                       GTATTACGCACCAGCGTCAGAACGTTTTTGTATTCACCCAGCGTTTTACCTACCGCCAGCGGAATCGCATCTTCCAGCGTTTTACGGATTTTCT                                                       <  SRR4302542.3255537/94‑1 (MQ=60)
                                       GTATTACGCACCAGCGTCAGAACGTTTTTGTATTCACCCAGCGTTTTACCTACCGCCAGCGGAATCGCATCTTCCAGCGTTTTACGGATTTTCT                                                       <  SRR4302542.4499270/94‑1 (MQ=60)
                                        TATTACGCACCAGCGTCAGAACGTTTTTGTATTCACCCAGCGTTTTACCTACCGCCAGCGGAATCGCATCTTCCAGCGTTTTACGGATTTTCTC                                                      >  SRR4302542.4461069/1‑94 (MQ=60)
                                        TATTACGCACCAGCGTCAGAACGTTTTTGTATTCACCCAGCGTTTTACCTACCACCAGCGGAATCGCATCTTCCAGCGTTTTACGGATTTTCTC                                                      >  SRR4302542.355302/1‑94 (MQ=60)
                                        TATTACGCACCAGCGTCAGAACGTTTTTGTATTCACCCAGCGTTTTACCTACCACCAGCGGAATCGCATCTTCCAGCGTTTTACGGATTTTCTC                                                      >  SRR4302542.3194236/1‑94 (MQ=60)
                                         ATTACGCACCAGCGTCAGAACGTTTTTGTATTCACCCAGCGTTTTACCTACCGCCAGCGGAATCGCATCTTCCAGCGTTTTACGGATTTTCTCG                                                     >  SRR4302542.4127867/1‑94 (MQ=60)
                                         ATTACGCACCAGCGTCAGAACGTTTTTGTATTCACCCAGCGTTTTACCTACCGCCAGCGGAATCGCATCTTCCAGCGTTTTACGGATTTTCTCG                                                     <  SRR4302542.515598/94‑1 (MQ=60)
                                         ATTACGCACCAGCGCCAGAACGTTTTTGTATTCACCCAGGGTTTTACCTCCCGCCAGCGGAATCGCATCTTCCAGCGTTTTACGGTTTTTCTCG                                                     >  SRR4302542.4614040/1‑94 (MQ=60)
                                         ATGACGCACCAGCGTCAGAACGGTCTTGTATTCACCCAGCGTTTTACCTACCGCCAGCGGAATCGCATCTTCCAGCGTTTTACGGATTTCCTCG                                                     <  SRR4302542.3412092/94‑1 (MQ=60)
                                           TACGCACCAGCGTCAGAACGTTTTTGTATTCACCCAGCGTTTTACCTACCGCCAGCGGAATCGCATCTTCCAGCGTTTTACGGATTTTCTCGCC                                                   <  SRR4302542.5513216/94‑1 (MQ=60)
                                             CGCACCAGCGTCAGAACGTTTTTGTATTCACCCAGCGTTTTACCTACCACCAGCGGAATCGCATCTTCCAGCGTTTTACGGATTTTCTCGCCGC                                                 >  SRR4302542.1442207/1‑94 (MQ=60)
                                              GCACCAGCGTCAGAACGTTTTTGTATTCACCCAGCGTTTTACCTACCGCCAGCGGAATCGCATCTTCCAGCGTTTTACGGATTTTCTCGCCGCC                                                <  SRR4302542.735802/94‑1 (MQ=60)
                                                ACCAGCGTCAGAACGTTTTTGTATTCACCCAGCGTTTTACCTACCGCCAGCGGAATCGCATCTTCCAGCGTTTTACGGATTTTCTCGCCGCCGG                                              >  SRR4302542.4069169/1‑94 (MQ=60)
                                                ACCAGCGTCAGAACGTTTTTGTATTCACCCAGCGTTTTACCTACCGCCAGCGGAATCGCATCTTCCAGCGTTTTACGGATTTTCTCGCCGCCGG                                              <  SRR4302542.3076050/94‑1 (MQ=60)
                                                ACCAGCGTCAGAACGTTTTTGTATTCACCCAGCGTTTTACCTACCGCCAGCGGAATCGCATCTTCCAGCGTTTTACGGATTTTCTCGCCGCCGG                                              >  SRR4302542.899712/1‑94 (MQ=60)
                                                ACCAGCGTCAGAACGTTTTTGTATTCACCCAGCGTTTTACCTACCGCCAGCGGAATCGCATCTTCCAGCGTTTTACGGATTTTCTCGCCGCCGG                                              >  SRR4302542.1863603/1‑94 (MQ=60)
                                                ACCAGCGTCAGAACGTTTTTGTATTCACCCAGCGTTTTACCTACCGCCAGCGGAATCGCATCTTCCAGCGTTTTACGGATTTTCTCGCCGCCGG                                              >  SRR4302542.467623/1‑94 (MQ=60)
                                                  CAGCGTCAGAACGTTTTTGTATTCACCCAGCGTTTTACCTACCGCCAGCGGAATCGCATCTTCCAGCGTTTTACGGATTTTCTCGCCGCCGGGA                                            >  SRR4302542.1124382/1‑94 (MQ=60)
                                                    GCGTCAGAACGTTTTTGTATTCACCCAGCGTTTTACCTACCGCCAGCGGAATCGCATCTTCCAGCGTTTTACGGATTTTCTCGCCGCCGGGAAT                                          <  SRR4302542.1076531/94‑1 (MQ=60)
                                                    GCGTCAGAACGTTTTTGTATTCACCCAGCGTTTTACCTACCGCCAGCGGAATCGCATCTTCCAGCGTTTTACGGATTTTCTCGCCGCCGGGAAT                                          <  SRR4302542.7188118/94‑1 (MQ=60)
                                                     CGTCAGAACGTTTTTGTATTCACCCAGCGTTTTACCTACCGCCAGCGGAATCGCATCTTCCAGCGTTTTACGGATTTTCTCGCCGCCGGGAATT                                         >  SRR4302542.6922761/1‑94 (MQ=60)
                                                     CGTCAGAACGTTTTTGTATTCACCCAGCGTTTTACCTACCGCCAGCGGAATCGCATCTTCCAGCGTTTTACGGATTTTCTCGCCGCCGGGAATT                                         <  SRR4302542.3498292/94‑1 (MQ=60)
                                                     CGTCAGAACGTTTTTGTATTCACCCAGCGTTTTACCTACCGCCAGCGGAATCGCATCTTCCAGCGTTTTACGGATTTTCTCGCCGCCGGGAATT                                         >  SRR4302542.3845832/1‑94 (MQ=60)
                                                     CGTCAGAACGTTTTTGTATTCACCCAGCGTTTTACCTACCGCCAGCGGAATCGCATCTTCCAGCGTTTTACGGATTTTCTCGCCGCCGGGAATT                                         >  SRR4302542.6645341/1‑94 (MQ=60)
                                                      GTCAGAACGTTTTTGTATTCACCCAGCGTTTTACCTACCGCCAGCGGAATCGCATCTTCCAGCGTTTTACGGATTTTCTCGCCGCCGGGAATTT                                        <  SRR4302542.6945181/94‑1 (MQ=60)
                                                      GTCAGAACGTTTTTGTATTCACCCAGCGTTTTACCTACCGCCAGCGGAATCGCATCTTCCAGCGTTTTACGGATTTTCTCGCCGCCGGGAATTT                                        >  SRR4302542.6900809/1‑94 (MQ=60)
                                                      GTCAGAACGTTTTTGTATTCACCCAGCGTTTTACCTACCACCAGCGGAATCGCATCTTCCAGCGTTTTACGGATTTTCTCGCCGCCGGGAATTT                                        <  SRR4302542.4006118/94‑1 (MQ=60)
                                                        CAGAACGTTTTTGTATTCACCCAGCGTTTTACCTACCGCCAGCGGAATCGCATCTTCCAGCGTTTTACGGATTTTCTCGCCGCCGGGAATTTCC                                      >  SRR4302542.407442/1‑94 (MQ=60)
                                                        CAGAACGTTTTTGTATTCACCCAGCGTTTTACCTACCGCCAGCGGAATCGCATCTTCCAGCGTTTTACGGATTTTCTCGCCGCCGGGAATTTCC                                      >  SRR4302542.1205408/1‑94 (MQ=60)
                                                        CAGAACGTTTTTGTATTCACCCAGCGTTTTACCTACCGCCAGCGGAATCGCATCTTCCAGCGTTTTACGGATTTTCTCGCCGCCGGGAATTTCC                                      >  SRR4302542.3615653/1‑94 (MQ=60)
                                                         AGAACGTTTTTGTATTCACTCAGCGTTTTACCTACCGCCAGCGGAATCGCATCTTCCAGCGTTTTACGGATTTTCTCGCCGCCGGGAATTTCCC                                     >  SRR4302542.5179263/1‑94 (MQ=60)
                                                         AGAACGTTTTTGTATTCACCCAGCGTTTTACCTACCGCCAGCGGAATCGCATCTTCCAGCGTTTTACGGATTTTCTCGCCGCCGGGAATTTCCC                                     >  SRR4302542.7053101/1‑94 (MQ=60)
                                                         AGAACGTTTTTGTATTCACCCAGCGTTTTACCTACCGCCAGCGGAATCGCATCTTCCAGCGTTTTACGGATTTTCTCGCCGCCGGGAATTTCCC                                     >  SRR4302542.820454/1‑94 (MQ=60)
                                                         AGAACGTTTTTGTATTCACCCAGCGTTTTACCTACCACCAGCGGAATCGCATCTTCCAGCGTTTTACGGATTTTCTCGCCGCCGGGAATTTCCC                                     >  SRR4302542.2302517/1‑94 (MQ=60)
                                                          GAACGTTTTTGTATTCACCCAGCGTTTTACCTACCGCCAGCGGAATCGCATCTTCCAGCGTTTTACGGATTTTCTCGCCGCCGGGAATTTCCCC                                    <  SRR4302542.4739690/94‑1 (MQ=60)
                                                           AACGTTTTTGTATTCACCCAGCGTTTTACCTACCGCCAGCGGAATCGCATCTTCCAGCGTTTTACGGATTTTCTCGCCGCCGGGAATTTCCCCT                                   >  SRR4302542.10236/1‑94 (MQ=60)
                                                            ACGTTTTTGTATTCACCCAGCGTTTTACCTACCGCCAGCGGAATCGCATCTTCCAGCGTTTTACGGATTTTCTCGCCGCCGGGAATTTCCCCTA                                  >  SRR4302542.1640464/1‑94 (MQ=60)
                                                              GTTTTTGTATTCACCCAGCGTTTTACCTACCGCCAGCGGAATCGCATCTTCCAGCGTTTTACGGATTTTCTCGCCGCCGGGAATTTCCCCTACG                                >  SRR4302542.4851820/1‑94 (MQ=60)
                                                              GTTTTTGTATTCACCCAGCGTTTTACCTACCGCCAGCGGAATCGCATCTTCCAGCGTTTTACGGATTTTCTCGCCGCCGGGAATTTCCCCTACG                                <  SRR4302542.1413615/94‑1 (MQ=60)
                                                              GTTTTTGTATTCACCCAGCGTTTTACCTACCACCAGCGGAATCGCATCTTCCAGCGTTTTACGGATTTTCTCGCCGCCGGGAATTTCCCCTACG                                >  SRR4302542.2253145/1‑94 (MQ=60)
                                                                TTTTGTATTCACCCAGCGTTTTACCTACCGCCAGCGGAATCGCATCTTCCAGCGTTTTACGGATTTTCTCGCCGCCGGGAATTTCCCCTACGCC                              >  SRR4302542.2715758/1‑94 (MQ=60)
                                                                 TTTGTATTCACCCAGCGTTTTACCTACCGCCAGCGGAATCGCATCTTCCAGCGTTTTACGGATTTTCTCGCCGCCGGGAATTTCCCCTACGCCA                             <  SRR4302542.5637960/94‑1 (MQ=60)
                                                                 TTTGTATTCACCCAGCGTTTTACCTACCGCCAGCGGAATCGCATCTTCCAGCGTTTTACGGATTTTCTCGCCGCCGGGAATTTCCCCTACGCCA                             <  SRR4302542.1596811/94‑1 (MQ=60)
                                                                  TTGTATTCACCCAGCGTTTTACCTACCGCCAGCGGAATCGCATCTTCCAGCGTTTTACGGATTTTCTCGCCGCCGGGAATTTCCCCTACGCCAG                            <  SRR4302542.4350911/94‑1 (MQ=60)
                                                                  TTGTATTCACCCAGCGTTTTACCTACCGCCAGCGGAATCGCATCTTCCAGCGTTTTACGGATTTTCTCGCCGCCGGGAATTTCCCCTACGCCAG                            >  SRR4302542.5815705/1‑94 (MQ=60)
                                                                  TTGTATTCACCCAGCGTTTTACCTACCGCCAGCGGAATCGCATCTTCCAGCGTTTTACGGATTTTCTCGCCGCCGGGAATTTCCCCTACGCCAG                            >  SRR4302542.3778872/1‑94 (MQ=60)
                                                                   TGTATTCACCCAGCGTTTTACCTACCGCCAGCGGAATCGCATCTTCCAGCGTTTTACGGATTTTCTCGCCGCCGGGAATTTCCCCTACGCCAGT                           >  SRR4302542.915302/1‑94 (MQ=60)
                                                                    GTATTCACCCAGCGTTTTACCTACCGCCAGCGGAATCGCATCTTCCAGCGTTTTACGGATTTTCTCGCCGCCGGGAATTTCCCCTACGCCAGTG                          >  SRR4302542.1294873/1‑94 (MQ=60)
                                                                     TATTCACCCAGCGTTTTACCTACCGCCAGCGGAATCGCATCTTCCAGCGTTTTACGGATTTTCTCGCCGCCGGGAATTTCCCCTACGCCAGTGT                         >  SRR4302542.225215/1‑94 (MQ=60)
                                                                      ATTCACCCAGCGTTTTACCTACCGCCAGCGGAATCGCATCTTCCAGCGTTTTACGGATTTTCTCGCCGCCGGGAATTTCCCCTACGCCAGTGTG                        <  SRR4302542.196433/94‑1 (MQ=60)
                                                                      ATTCACCCAGCGTTTTACCTACCACCAGCGGAATCGCATCTTCCAGCGTTTTACGGATTTTCTCGCCGCCGGGAATTTCCCCTACGCCAGTGTG                        <  SRR4302542.1379906/94‑1 (MQ=60)
                                                                         CACCCAGCGTTTTACCTACCGCCAGCGGAATCGCATCTTCCAGCGTTTTACGGATTTTCTCGCCGCCGGGAATTTCCCCTACGCCAGTGTGACC                     >  SRR4302542.6576528/1‑94 (MQ=60)
                                                                          ACCCAGCGTTTTACCTACCGCCAGCGGAATCGCATCTTCCAGCGTTTTACGGATTTTCTCGCCGCCGGGAATTTCCCCTACGCCAGTGTGACCA                    >  SRR4302542.2819453/1‑94 (MQ=60)
                                                                           CCCAGCGTTTTACCTACCGCCAGCGGAATCGCATCTTCCAGCGTTTTACGGATTTTCTCGCCGCCGGGAATTTCCCCTACGCCAGTGTGACCAG                   >  SRR4302542.6126136/1‑94 (MQ=60)
                                                                           CCCAGCGTTTTACCTACCGCCAGCGGAATCGCATCTTCCAGCGTTTTACGGATTTTCTCGCCGCCGGGAATTTCCCCTACGCCAGTGTGACCAG                   >  SRR4302542.553075/1‑94 (MQ=60)
                                                                           CCCAGCGTTTTACCTACCGCCAGCGGAATCGCATCTTCCAGCGTTTTACGGATTTTCTCGCCGCCGGGAATTTCCCCTACGCCAGTGTGACCAG                   >  SRR4302542.1983227/1‑94 (MQ=60)
                                                                           CCCAGCGTTTTACCTACCGCCAGCGGAATCGCATCTTCCAGCGTTTTACGGATTTTCTCGCCGCCGGGAATTTCCCCTACGCCAGTGTGACCAG                   <  SRR4302542.788965/94‑1 (MQ=60)
                                                                           CCCAGCGTTTTACCTACCGCCAGCGGAATCGCATCTTCCAGCGTTTTACGGATTTTCTCGCCGCCGGGAATTTCCCCTACGCCAGTGTGACCAG                   <  SRR4302542.4739680/94‑1 (MQ=60)
                                                                              AGCGTTTTACCTACCGCCAGCGGAATCGCATCTTCCAGCGTTTTACGGATTTTCTCGCCGCCGGGAATTTCCCCTACGCCAGTGTGACCAGAAT                <  SRR4302542.5903662/94‑1 (MQ=60)
                                                                               GCGTTTTACCTACCGCCAGCGGAATCGCATCTTCCAGCGTTTTACGGATTTTCTCGCCGCCGGGAATTTCCCCTACGCCAGTGTGACCAGAATT               <  SRR4302542.997208/94‑1 (MQ=60)
                                                                               GCGTTTTACCTACCGCCAGCGGAATCGCATCTTCCAGCGTTTTACGGATTTTCTCGCCGCCGGGAATTTCCCCTACGCCAGTGTGACCAGAATT               <  SRR4302542.2942067/94‑1 (MQ=60)
                                                                               GCGTTTTACCTACCGCCAGCGGAATCGCATCTTCCAGCGTTTTACGGATTTTCTCGCCGCCGGGAATTTCCCCTACGCCAGTGTGACCAGAATT               <  SRR4302542.5586326/94‑1 (MQ=60)
                                                                                CGTTTTACCTACCGCCAGCGGAATCGCATCTTCCAGCGTTTTACGGATTTTCTCGCCGCCGGGAATTTCCCCTACGCCAGTGTGACCAGAATTA              <  SRR4302542.4538721/94‑1 (MQ=60)
                                                                                CGTTTTACCTACCGCCAGCGGAATCGCATCTTCCAGCGTTTTACGGATTTTCTCGCCGCCGGGAATTTCCCCTACGCCAGTGTGACCAGAATTA              >  SRR4302542.5248813/1‑94 (MQ=60)
                                                                                 GTTTTACCTACCGCCAGCGGAATCGCATCTTCCAGCGTTTTACGGATTTTCTCGCCGCCGGGAATTTCCCCTACGCCAGTGTGACCAGAATTAT             >  SRR4302542.5013619/1‑94 (MQ=60)
                                                                                 GTTTTACCTACCGCCAGCGGAATCGCATCTTCCAGCGTTTTACGGATTTTCTCGCCGCCGGGAATTTCCCCTACGCCAGTGTGACCAGAATTAT             <  SRR4302542.1086976/94‑1 (MQ=60)
                                                                                 GTTTTACCTACCGCCAGCGGAATCGCATCTTCCAGCGTTTTACGGATTTTCTCGCCGCCGGGAATTTCCCCTACGCCAGTGTGACCAGAATTAT             <  SRR4302542.2936577/94‑1 (MQ=60)
                                                                                 GTTTTACCTACCGCCAGCGGAATCGCATCTTCCAGCGTTTTACGGATTTTCTCGCCGCCGGGAATTTCCCCTACGCCAGTGTGACCAGAATTAT             >  SRR4302542.2820389/1‑94 (MQ=60)
                                                                                 GTTTTACCTACCGCCAGCGGAATCGCATCTTCCAGCGTTTTACGGATTTTCTCGCCGCCGGGAATTTCCCCTACGCCAGTGTGACCAGAATTAT             >  SRR4302542.5848323/1‑94 (MQ=60)
                                                                                    TTACCTACCGCCAGCGGAATCGCATCTTCCAGCGTTTTACGGATTTTCTCGCCGCCGGGAATTTCCCCTACGCCAGTGTGACCAGAATTATCTT          <  SRR4302542.1256370/94‑1 (MQ=60)
                                                                                    TTACCTACCGCCAGCGGAATCGCATCTTCCAGCGTTTTACGGATTTTCTCGCCGCCGGGAATTTCCCCTACGCCAGTGTGACCAGAATTATCTT          <  SRR4302542.7153675/94‑1 (MQ=60)
                                                                                    TTACCTACCGCCAGCGGAATCGCATCTTCCAGCGTTTTACGGATTTTCTCGCCGCCGGGAATTTCCCCTACGCCAGTGTGACCAGAATTATCTT          >  SRR4302542.206917/1‑94 (MQ=60)
                                                                                     TACCTACCGCCAGCGGAATCGCATCTTCCAGCGTTTTACGGATTTTCTCGCCGCCGGGAATTTCCCCTACGCCAGTGTGACCAGAATTATCTTT         <  SRR4302542.2432440/94‑1 (MQ=60)
                                                                                      ACCTACCGCCAGCGGAATCGCATCTTCCAGCGTTTTACGGATTTTCTCGCCGCCGGGAATTTCCCCTACGCCAGTGTGACCAGAATTATCTTTG        <  SRR4302542.3167555/94‑1 (MQ=60)
                                                                                       CCTACCGCCAGCGGAATCGCATCTTCCAGCGTTTTACGGATTTTCTCGCCGCCGGGAATTTCCCCTACGCCAGTGTGACCAGAATTATCTTTGA       <  SRR4302542.3384159/94‑1 (MQ=60)
                                                                                       CCTACCGCCAGCGGAATCGCATCTTCCAGCGTTTTACGGATTTTCTCGCCGCCGGGAATTTCCCCTACGCCAGTGTGACCAGAATTATCTTTGA       <  SRR4302542.598579/94‑1 (MQ=60)
                                                                                        CTACCGCCAGCGGAATCGCATCTTCCAGCGTTTTACGGATTTTCTCGCCGCCGGGAATTTCCCCTACGCCAGTGTGACCAGAATTATCTTTGAT      <  SRR4302542.2883308/94‑1 (MQ=60)
                                                                                        CTACCGCCAGCGGAATCGCATCTTCCAGCGTTTTACGGATTTTCTCGCCGCCGGGAATTTCCCCTACGCCAGTGTGACCAGAATTATCTTTGAT      <  SRR4302542.3045334/94‑1 (MQ=60)
                                                                                        CTACCGCCAGCGGAATCGCATCTTCCAGCGTTTTACGGATTTTCTCGCCGCCGGGAATTTCCCCTACACCAGTGTGACCAGAATTATCTTTGAT      >  SRR4302542.5555602/1‑94 (MQ=60)
                                                                                         TACCGCCAGCGGAATCGCATCTTCCAGCGTTTTACGGATTTTCTCGCCGCCGGGAATTTCCCCTACGCCAGTGTGACCAGAATTATCTTTGATA     >  SRR4302542.3296111/1‑94 (MQ=60)
                                                                                           CCGCCAGCGGAATCGCATCTTCCAGCGTTTTACGGATTTTCTCGCCGCCGGGAATTTCCCCTACGCCAGTGTGACCAGAATTATCTTTGATAAT   >  SRR4302542.5894755/1‑94 (MQ=60)
                                                                                           CCGCCAGCGGAATCGCATCTTCCAGCGTTTTACGGATTTTCTCGCCGCCGGGAATTTCCCCTACGCCAGTGTGACCAGAATTATCTTTGATAAT   >  SRR4302542.6319079/1‑94 (MQ=60)
                                                                                            CGCCAGCGGAATCGCATCTTCCAGCGTTTTACGGATTTTCTCGCCGCCGGGAATTTCCCCTACGCCAGTGTGACCAGAATTATCTTTGATAATC  <  SRR4302542.5889308/94‑1 (MQ=60)
                                                                                             |                                                                                            
GTCTGCAAACCGCGCCCACCAGCATCACGATCGGCAAAAGTATTACGCACCAGCGTCAGAACGTTTTTGTATTCACCCAGCGTTTTACCTACCACCAGCGGAATCGCATCTTCCAGCGTTTTACGGATTTTCTCGCCGCCGGGAATTTCCCCTACGCCAGTGTGACCAGAATTATCTTTGATAATC  >  NC_000913/2917100‑2917285

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑