Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1061 294.1 15065038 98.6% 14854127 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,077,340 T→C S4G (AGT→GGT)  cmtB ← PTS system mannitol‑specific enzyme IIA component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,077,3400TC81.1% 416.5 / 106.2 212S4G (AGT→GGT) cmtBPTS system mannitol‑specific enzyme IIA component
Reads supporting (aligned to +/- strand):  ref base T (10/30);  new base C (53/119);  total (63/149)
Fisher's exact test for biased strand distribution p-value = 5.66e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.92e-03

Only 100 of 212 total aligned reads displayed.

TCCAGCAATGATACCATCGAGAAATCGATAGCTTCCTGCCAATCTTTTGCTGAATGTATAACTGAGATTGATGATTCTGGAAAATAATCACTAAGCCGCATAAATTATCCTTATTTTATTTTTAGGGTACCGTCCGGCGGTAAAAATAAAATACTCGTGCGGTTTAATGAAAGCAATTGAG  >  NC_000913/3077249‑3077429
                                                                                           |                                                                                         
tCCAGCAATGATACCATCGAGAAATCGATAGCTTCCTGCCAATCTTTTGCTGAATGTATAACTGAGATTGATGATTCTGGAAAATAATCACTa                                                                                          <  2:642669/93‑1 (MQ=255)
 ccAGCAATGATACCATCGAGAAATCGATAGCTTCCTGCCAATCTTTTGCTGAATGTATAACTGAGATTGATGATTCTGGAAAATAATCACCaa                                                                                         <  2:3355373/93‑1 (MQ=255)
  cAGCAATGATACCATCGAGAAATCGATAGCTTCCTGCCAATCTTTTGCTGAATGTATAACTGAGATTGATGATTCTGGAAAATAATCACTAAg                                                                                        >  2:4937453/1‑93 (MQ=255)
    gCAATGATACCATCGAGAAATCGATAGCTTCCTGCCAATCTTTTGCTGAATGTATAACTGAGATTGATGATTCTGGAAAATAATCACCAAGcc                                                                                      <  2:5157498/93‑1 (MQ=255)
     cAATGATACCATCGAGAAATCGATAGCTTCCTGCCAATCTTTTGCTGAATGTATAACTGAGATTGATGATTCTGGAAAATAATCACCAAGCCg                                                                                     <  2:3955878/93‑1 (MQ=255)
      aaTGATACCATCGAGAAATCGATAGCTTCCTGCCAATCTTTTGCTGAATGTATAACTGAGATTGATGATTCTGGAAAATAATCACTAAGCCGc                                                                                    <  1:5266948/93‑1 (MQ=255)
      aaTGATACCATCGAGAAATCGATAGCTTCCTGCCAATCTTTTGCTGAATGTATAACTGAGATTGATGATTCTGGAAAATAATCACCAAGCCGc                                                                                    >  1:6007807/1‑93 (MQ=255)
      aaTGATACCATCGAGAAATCGATAGCTTCCTGCCAATCTTTTGCTGAATGTATAACTGAGATTGATGATTCTGGAAAATAATCACCAAGCCGc                                                                                    <  2:1983313/93‑1 (MQ=255)
       aTGATACCATCGAGAAATCGATAGCTTCCTGCCAATCTTTTGCTGAATGTATAACTGAGATTGATGATTCTGGAAAATAATCACCAAGCCGCa                                                                                   <  1:6108470/93‑1 (MQ=255)
       aTGATACCATCGAGAAATCGATAGCTTCCTGCCAATCTTTTGCTGAATGTATAACTGAGATTGATGATTCTGGAAAATAATCACCAAGCCGCa                                                                                   <  2:224237/93‑1 (MQ=255)
       aTGATACCATCGAGAAATCGATAGCTTCCTGCCAATCTTTTGCTGAATGTATAACTGAGATTGATGATTCTGGAAAATAATCACCAAGCCGCa                                                                                   <  1:3586229/93‑1 (MQ=255)
        tGATACCATCGAGAAATCGATAGCTTCCTGCCAATCTTTTGCTGAATGTATAACTGAGATTGATGATTCTGGAAAATAATCACCAAGCCGCAt                                                                                  >  2:5544742/1‑93 (MQ=255)
         gATACCATCGAGAAATCGATAGCTTCCTGCCAATCTTTTGCTGAATGTATAACTGAGATTGATGATTCTGGAAAATAATCACCAAGCCGCATa                                                                                 <  2:5760050/93‑1 (MQ=255)
          aTACCATCGAGAAATCGATAGCTTCCTGCCAATCTTTTGCTGAATGTATAACTGAGATTGATGATTCTGGAAAATAATCACCAAGCCGCATaa                                                                                >  1:7171534/1‑93 (MQ=255)
           tACCATCGAGAAATCGATAGCTTCCTGCCAATCTTTTGCTGAATGTATAACTGAGATTGATGATTCTGGAAAATAATCACCAAGCCGCATaaa                                                                               <  2:6136491/93‑1 (MQ=255)
           tACCATCGAGAAATCGATAGCTTCCTGCCAATCTTTTGCTGAATGTATAACTGAGATTGATGATTCTGGAAAATAATCACCAAGCCGCATaaa                                                                               <  1:1661244/93‑1 (MQ=255)
            aCCATCGAGAAATCGATAGCTTCCTGCCAATCTTTTGCTGAATGTATAACTGAGATTGATGATTCTGGAAAATAATCACCAAGCCGCATAAAt                                                                              <  1:5031268/93‑1 (MQ=255)
             ccATCGAGAAATCGATAGCTTCCTGCCAATCTTTTGCTGAATGTATAACTGAGATTGATGATTCTGGAAAATAATCACCAAGCCGCATAAAtt                                                                             >  1:4215038/1‑93 (MQ=255)
              cATCGAGAAATCGATAGCTTCCTGCCAATCTTTTGCTGAATGTATAACTGAGATTGATGATTCTGGAAAATAATCACTAAGCCGCATAAATTa                                                                            <  1:7312933/93‑1 (MQ=255)
                tCGAGAAATCGATAGCTTCCTGCCAATCTTTTGCTGAATGTATAACTGAGATTGATGATTCTGGAAAATAATCACCAAGCCGCATAAATTATc                                                                          <  2:874710/93‑1 (MQ=255)
                 cGAGAAATCGATAGCTTCCTGCCAATCTTTTGCTGAATGTATAACTGAGATTGATGATTCTGGAAAATAATCACCAAGCCGCATAAATTATcc                                                                         <  1:4127107/93‑1 (MQ=255)
                  gagaAATCGATAGCTTCCTGCCAATCTTTTGCTGAATGTATAACTGAGATTGATGATTCTGGAAAATAATCACCAAGCCGCATAAATTATCCt                                                                        <  1:299799/93‑1 (MQ=255)
                   agaAATCGATAGCTTCCTGCCAATCTTTTGCTGAATGTATAACTGAGATTGATGATTCTGGAAAATAATCACCAAGCCGCATAAATTATCCtt                                                                       >  2:384791/1‑93 (MQ=255)
                   agaAATCGATAGCTTCCTGCCAATCTTTTGCTGAATGTATAACTGAGATTGATGATTCTGGAAAATAATCACCAAGCCGCATAAATTATCCtt                                                                       <  1:847679/93‑1 (MQ=255)
                   agaAATCGATAGCTTCCTGCCAATCTTTTGCTGAATGTATAACTGAGATTGATGATTCTGGAAAATAATCACCAAGCCGCATAAATTATCCtt                                                                       <  1:4732256/93‑1 (MQ=255)
                      aaTCGATAGCTTCCTGCCAATCTTTTGCTGAATGTATAACTGAGATTGATGATTCTGGAAAATAATCACCAAGCCGCATAAATTATCCttatt                                                                    >  2:3664790/1‑93 (MQ=255)
                      aaTCGATAGCTTCCTGCCAATCTTTTGCTGAATGTATAACTGAGATTGATGATTCTGGAAAATAATCACCAAGCCGCATAAATTATCCttatt                                                                    <  1:574677/93‑1 (MQ=255)
                       aTCGATAGCTTCCTGCCAATCTTTTGCTGAATGTATAACTGAGATTGATGATTCTGGAAAATAATCACCAAGCCGCATAAATTATCCttattt                                                                   <  2:6748069/93‑1 (MQ=255)
                        tCGATAGCTTCCTGCCAATCTTTTGCTGAATGTATAACTGAGATTGATGATTCTGGAAAATAATCACCAAGCCGCATAAATTATCCttatttt                                                                  <  2:2470531/93‑1 (MQ=255)
                        tCGATAGCTTCCTGCCAATCTTTTGCTGAATGTATAACTGAGATTGATGATTCTGGAAAATAATCACCAAGCCGCATAAATTATCCttatttt                                                                  <  1:6194034/93‑1 (MQ=255)
                        tCGATAGCTTCCTGCCAATCTTTTGCTGAATGTATAACTGAGATTGATGATTCTGGAAAATAATCACCAAGCCGCATAAATTATCCttatttt                                                                  <  1:2360448/93‑1 (MQ=255)
                        tCGATAGCTTCCTGCCAATCTTTTGCTGAATGTATAACTGAGATTGATGATTCTGGAAAATAATCACCAAGCCGCATAAATTATCCttatttt                                                                  <  1:2565304/93‑1 (MQ=255)
                         cGATAGCTTCCTGCCAATCTTTTGCTGAATGTATAACTGAGATTGATGATTCTGGAAAATAATCACTAAGCCGCATAAATTATCCttatttta                                                                 <  1:982763/93‑1 (MQ=255)
                         cGATAGCTTCCTGCCAATCTTTTGCTGAATGTATAACTGAGATTGATGATTCTGGAAAATAATCACCAAGCCGCATAAATTATCCttatttta                                                                 <  1:2707153/93‑1 (MQ=255)
                          gATAGCTTCCTGCCAATCTTTTGCTGAATGTATAACTGAGATTGATGATTCTGGAAAATAATCACCAAGCCGCATAAATTATCCttattttat                                                                <  1:1373760/93‑1 (MQ=255)
                           aTAGCTTCCTGCCAATCTTTTGCTGAATGTATAACTGAGATTGATGATTCTGGAAAATAATCACTAAGCCGCATAAATTATCCttattttatt                                                               <  2:4278386/93‑1 (MQ=255)
                           aTAGCTTCCTGCCAATCTTTTGCTGAATGTATAACTGAGATTGATGATTCTGGAAAATAATCACCAAGCCGCATAAATTATCCttattttatt                                                               <  1:2023657/93‑1 (MQ=255)
                           aTAGCTTCCTGCCAATCTTTTGCTGAATGTATAACTGAGATTGATGATTCTGGAAAATAATCACCAAGCCGCATAAATTATCCttattttatt                                                               <  2:5776365/93‑1 (MQ=255)
                           aTAGCTTCCTGCCAATCTTTTGCTGAATGTATAACTGAGATTGATGATTCTGGAAAATAATCACCAAGCCGCATAAATTATCCttattttatt                                                               <  1:1402720/93‑1 (MQ=255)
                           aTAGCTTCCTGCCAATCTTTTGCTGAATGTATAACTGAGATTGATGATTCTGGAAAATAATCACCAAGCCGCATAAATTATCCttattttatt                                                               >  1:4520584/1‑93 (MQ=255)
                            tAGCTTCCTGCCAATCTTTTGCTGAATGTATAACTGAGATTGATGATTCTGGAAAATAATCACTAAGCCGCATAAATTATCCTtattttattt                                                              >  2:4760280/1‑93 (MQ=255)
                            tAGCTTCCTGCCAATCTTTTGCTGAATGTATAACTGAGATTGATGATTCTGGAAAATAATCACCAAGCCGCATAAATTATCCTtattttattt                                                              <  1:4672795/93‑1 (MQ=255)
                            tAGCTTCCTGCCAATCTTTTGCTGAATGTATAACTGAGATTGATGATTCTGGAAAATAATCACCAAGCCGCATAAATTATCCTtattttattt                                                              <  1:3246562/93‑1 (MQ=255)
                            tAGCTTCCTGCCAATCTTTTGCTGAATGTATAACTGAGATTGATGATTCTGGAAAATAATCACCAAGCCGCATAAATTATCCTtattttattt                                                              <  1:3353738/93‑1 (MQ=255)
                              gCTTCCTGCCAATCTTTTGCTGAATGTATAACTGAGATTGATGATTCTGGAAAATAATCACCAAGCCGCATAAATTATCCTTATTTTAttttt                                                            <  2:2741922/93‑1 (MQ=255)
                              gCTTCCTGCCAATCTTTTGCTGAATGTATAACTGAGATTGATGATTCTGGAAAATAATCACCAAGCCGCATAAATTATCCTTATTTTAttttt                                                            <  2:1169021/93‑1 (MQ=255)
                              gCTTCCTGCCAATCTTTTGCTGAATGTATAACTGAGATTGATGATTCTGGAAAATAATCACCAAGCCGCATAAATTATCCTTATTTTAttttt                                                            >  1:1789722/1‑93 (MQ=255)
                               cTTCCTGCCAATCTTTTGCTGAATGTATAACTGAGATTGATGATTCTGGAAAATAATCACCAAGCCGCATAAATTATCCTTATTTTATTTTTa                                                           <  2:1731886/93‑1 (MQ=255)
                               cTTCCTGCCAATCTTTTGCTGAATGTATAACTGAGATTGATGATTCTGGAAAATAATCACCAAGCCGCATAAATTATCCTTATTTTATTTTTa                                                           <  2:7412691/93‑1 (MQ=255)
                               cTTCCTGCCAATCTTTTGCTGAATGTATAACTGAGATTGATGATTCTGGAAAATAATCACCAAGCCGCATAAATTATCCTTATTTTATTTTTa                                                           >  2:7378415/1‑93 (MQ=255)
                                  ccTGCCAATCTTTTGCTGAATGTATAACTGAGATTGATGATTCTGGAAAATAATCACCAAGCCGCATAAATTATCCTTATTTTATTTTTAggg                                                        <  1:3401326/93‑1 (MQ=255)
                                  ccTGCCAATCTTTTGCTGAATGTATAACTGAGATTGATGATTCTGGAAAATAATCACCAAGCCGCATAAATTATCCTTATTTTATTTTTAggg                                                        >  2:4026636/1‑93 (MQ=255)
                                  ccTGCCAATCTTTTGCTGAATGTATAACTGAGATTGATGATTCTGGAAAATAATCACCAAGCCGCATAAATTATCCTTATTTTATTTTTAggg                                                        >  2:1532606/1‑93 (MQ=255)
                                  ccTGCCAATCTTTTGCTGAATGTATAACTGAGATTGATGATTCTGGAAAATAATCACCAAGCCGCATAAATTATCCTTATTTTATTTTTAggg                                                        <  1:3931938/93‑1 (MQ=255)
                                    tGCCAATCTTTTGCTGAATGTATAACTGAGATTGATGATTCTGGAAAATAATCACCAAGCCGCATAAATTATCCTTATTTTATTTTTAGGGTa                                                      >  1:6637138/1‑93 (MQ=255)
                                    tGCCAATCTTTTGCTGAATGTATAACTGAGATTGATGATTCTGGAAAATAATCACCAAGCCGCATAAATTATCCTTATTTTATTTTTAGGGTa                                                      >  1:405737/1‑93 (MQ=255)
                                     gCCAATCTTTTGCTGAATGTATAACTGAGATTGATGATTCTGGAAAATAATCACTAAGCCGCATAAATTATCCTTATTTTATTTTTAGGGTAc                                                     <  2:7120306/93‑1 (MQ=255)
                                     gCCAATCTTTTGCTGAATGTATAACTGAGATTGATGATTCTGGAAAATAATCACCAAGCCGCATAAATTATCCTTATTTTATTTTTAGGGTAc                                                     <  1:4833058/93‑1 (MQ=255)
                                     gCCAATCTTTTGCTGAATGTATAACTGAGATTGATGATTCTGGAAAATAATCACCAAGCCGCATAAATTATCCTTATTTTATTTTTAGGGTAc                                                     <  1:6657527/93‑1 (MQ=255)
                                      ccAATCTTTTGCTGAATGTATAACTGAGATTGATGATTCTGGAAAATAATCACTAAGCCGCATAAATTATCCTTATTTTATTTTTAGGGTAcc                                                    >  1:3974955/1‑93 (MQ=255)
                                         aTCTTTTGCTGAATGTATAACTGAGATTGATGATTCTGGAAAATAATCACCAAGCCGCATAAATTATCCTTATTTTATTTTTAGGGTACCGTc                                                 <  2:7492189/93‑1 (MQ=255)
                                         aTCTTTTGCTGAATGTATAACTGAGATTGATGATTCTGGAAAATAATCACCAAGCCGCATAAATTATCCTTATTTTATTTTTAGGGTACCGTc                                                 <  2:2203274/93‑1 (MQ=255)
                                            ttttGCTGAATGTATAACTGAGATTGATGATTCTGGAAAATAATCACCAAGCCGCATAAATTATCCTTATTTTATTTTTAGGGTACCGTCcgg                                              <  1:4168674/93‑1 (MQ=255)
                                            ttttGCTGAATGTATAACTGAGATTGATGATTCTGGAAAATAATCACCAAGCCGCATAAATTATCCTTATTTTATTTTTAGGGTACCGTCcgg                                              >  1:6684601/1‑93 (MQ=255)
                                             tttGCTGAATGTATAACTGAGATTGATGATTCTGGAAAATAATCACCAAGCCGCATAAATTATCCTTATTTTATTTTTAGGGTACCGTCcggc                                             <  2:1569216/93‑1 (MQ=255)
                                                gCTGAATGTATAACTGAGATTGATGATTCTGGAAAATAATCACTAAGCCGCATAAATTATCCTTATTTTATTTTTAGGGTACCGTCCGGCGGt                                          <  2:6036600/93‑1 (MQ=255)
                                                 cTGAATGTATAACTGAGATTGATCATTCTGGAAAATAATCACTAAGCCGCATAAATTATCCTTATTTTATTTTTAGGGTACCGTCCAGCGGTa                                         <  2:6970052/93‑1 (MQ=255)
                                                   gAATGTATAACTGAGATTGATGATTCTGGAAAATAATCACCAAGCCGCATAAATTATCCTTATTTTATTTTTAGGGTACCGTCCGGCGGTaaa                                       <  2:6645903/93‑1 (MQ=255)
                                                      tGTATAACTGAGATTGATGATTCTGGAAAATAATCACTAAGCCGCATAAATTATCCTTATTTTATTTTTAGGGTACCGTCCGGCGGTAaaaat                                    >  2:201431/1‑93 (MQ=255)
                                                      tGTATAACTGAGATTGATGATTCTGGAAAATAATCACCAAGCCGCATAAATTATCCTTATTTTATTTTTAGGGTACCGTCCGGCGGTAaaaat                                    >  1:6413906/1‑93 (MQ=255)
                                                         ataACTGAGATTGATGATTCTGGAAAATAATCACCAAGCCGCATAAATTATCCTTATTTTATTTTTAGGGTACCGTCCGGCGGTAaaaataaa                                 >  1:3716754/1‑93 (MQ=255)
                                                          taACTGAGATTGATGATTCTGGAAAATAATCACCAAGCCGCATAAATTATCCTTATTTTATTTTTAGGGTACCGTCCGGCGGTAaaaataaaa                                <  1:54256/93‑1 (MQ=255)
                                                            aCTGAGATTGATGATTCTGGAAAATAATCACCAAGCCGCATAAATTATCCTTATTTTATTTTTAGGGTACCGTCCGGCGGTAAaaataaaata                              <  2:1694582/93‑1 (MQ=255)
                                                            aCTGAGATTGATGATTCTGGAAAATAATCACCAAGCCGCATAAATTATCCTTATTTTATTTTTAGGGTACCGTCCGGCGGTAAaaataaaata                              <  1:6315029/93‑1 (MQ=255)
                                                            aCTGAGATTGATGATTCTGGAAAATAATCACCAAGCCGCATAAATTATCCTTATTTTATTTTTAGGGTACCGTCCGGCGGTAAaaataaaata                              <  1:5566764/93‑1 (MQ=255)
                                                            aCTGAGATTGATGATTCTGGAAAATAATCACCAAGCCGCATAAATTATCCTTATTTTATTTTTAGGGTACCGTCCGGCGGTAAaaataaaata                              >  2:340694/1‑93 (MQ=255)
                                                               gagaTTGATGATTCTGGAAAATAATCACCAAGCCGCATAAATTATCCTTATTTTATTTTTAGGGTACCGTCCGGCGGTAAAAATAAAATACTc                           <  1:5970682/93‑1 (MQ=255)
                                                                 gaTTGATGATTCTGGAAAATAATCACTAAGCCGCATAAATTATCCTTATTTTATTTTTAGGGTACCGTCCGGCGGTAAAAATAAAATACTCGt                         <  2:6868109/93‑1 (MQ=255)
                                                                 gaTTGATGATTCTGGAAAATAATCACTAAGCCGCATAAATTATCCTTATTTTATTTTTAGGGTACCGTCCGGCGGTAAAAATAAAATACTCGt                         <  2:1938590/93‑1 (MQ=255)
                                                                  aTTGATGATTCTGGAAAATAATCACCAAGCCGCATAAATTATCCTTATTTTATTTTTAGGGTACCGTCCGGCGGTAAAAATAAAATACTCGTg                        <  1:292872/93‑1 (MQ=255)
                                                                   ttGATGATTCTGGAAAATAATCACTAAGCCGCATAAATTATCCTTATTTTATTTTTAGGGTACCGTCCGGCGGTAAAAATAAAATACTCGTGc                       <  2:4688952/93‑1 (MQ=255)
                                                                    tgatgaTTCTGGAAAATAATCACCAAGCCGCATAAATTATCCTTATTTTATTTTTAGGGTACCGTCCGGCGGTAAAAATAAAATACTCGTGCg                      <  2:4113763/93‑1 (MQ=255)
                                                                     gatgatTCTGGAAAATAATCACCAAGCCGCATAAATTATCCTTATTTTATTTTTAGGGTACCGTCCGGCGGTAAAAATAAAATACTCGTGCgg                     >  2:5247937/1‑93 (MQ=255)
                                                                        gatTCTGGAAAATAATCACCAAGCCGCATAAATTATCCTTATTTTATTTTTAGGGTACCGTCCGGCGGTAAAAATAAAATACTCGTGCGGttt                  >  2:2356589/1‑93 (MQ=255)
                                                                        gatTCTGGAAAATAATCACCAAGCCGCATAAATTATCCTTATTTTATTTTTAGGGTACCGTCCGGCGGTAAAAATAAAATACTCGTGCGGttt                  <  2:5859562/93‑1 (MQ=255)
                                                                           tCTGGAAAATAATCACTAAGCCGCATAAATTATCCTTATTTTATTTTTAGGGTACCGTCCGGCGGTAAAAATAAAATACTCGTGCGGTTTAAt               <  2:3775844/93‑1 (MQ=255)
                                                                           tCTGGAAAATAATCACCAAGCCGCATAAATTATCCTTATTTTATTTTTAGGGTACCGTCCGGCGGTAAAAATAAAATACTCGTGCGGTTTAAt               <  1:1154820/93‑1 (MQ=255)
                                                                            cTGGAAAATAATCACCAAGCCGCATAAATTATCCTTATTTTATTTTTAGGGTACCGTCCGGCGGTAAAAATAAAATACTCGTGCGGTTTAATg              <  1:6748575/93‑1 (MQ=255)
                                                                             tGGAAAATAATCACCAAGCCGCATAAATTATCCTTATTTTATTTTTAGGGTACCGTCCGGCGGTAAAAATAAAATACTCGTGCGGTTTAATGa             <  1:3824495/93‑1 (MQ=255)
                                                                             tGGAAAATAATCACCAAGCCGCATAAATTATCCTTATTTTATTTTTAGGGTACCGTCCGGCGGTAAAAATAAAATACTCGTGCGGTTTAATGa             >  2:1106083/1‑93 (MQ=255)
                                                                             tGGAAAATAATCACCAAGCCGCATAAATTATCCTTATTTTATTTTTAGGGTACCGTCCGGCGGTAAAAATAAAATACTCGTGCGGTTTAATGa             <  2:4380366/93‑1 (MQ=255)
                                                                              ggAAAATAATCACCAAGCCGCATAAATTATCCTTATTTTATTTTTAGGGTACCGTCCGGCGGTAAAAATAAAATACTCGTGCGGTTTAATGaa            <  2:3223014/93‑1 (MQ=255)
                                                                               gAAAATAATCACTAAGCCGCATAAATTATCCTTATTTTATTTTTAGGGTACCGTCCGGCGGTAAAAATAAAATACTCGTGCGGTTTAATGaaa           <  1:4937453/93‑1 (MQ=255)
                                                                               gAAAATAATCACCAAGCCGCATAAATTATCCTTATTTTATTTTTAGGGTACCGTCCGGCGGTAAAAATAAAATACTCGTGCGGTTTAATGaaa           <  1:5492123/93‑1 (MQ=255)
                                                                                aaaaTAATCACTAAGCCGCATAAATTATCCTTATTTTATTTTTAGGGTACCGTCCGGCGGTAAAAATAAAATACTCGTGCGGTTTAATGAAAg          <  2:4615501/93‑1 (MQ=255)
                                                                                   ataatCACCAAGCCGCATAAATTATCCTTATTTTATTTTTAGGGTACCGTCCGGCGGTAAAAATAAAATACTCGTGCGGTTTAATGAAAGCaa       <  1:4451988/93‑1 (MQ=255)
                                                                                   ataatCACCAAGCCGCATAAATTATCCTTATTTTATTTTTAGGGTACCGTCCGGCGGTAAAAATAAAATACTCGTGCGGTTTAATGAAAGCaa       <  2:1141108/93‑1 (MQ=255)
                                                                                    taatCACCAAGCCGCATAAATTATCCTTATTTTATTTTTAGGGTACCGTCCGGCGGTAAAAATAAAATACTCGTGCGGTTTAATGAAAGCAAt      >  1:6926469/1‑93 (MQ=255)
                                                                                     aatCACCAAGCCGCATAAATTATCCTTATTTTATTTTTAGGGTACCGTCCGGCGGTAAAAATAAAATACTCGTGCGGTTTAATGAAAGCAAtt     <  1:4654895/93‑1 (MQ=255)
                                                                                       tCACCAAGCCGCATAAATTATCCTTATTTTATTTTTAGGGTACCGTCCGGCGGTAAAAATAAAATACTCGTGCGGTTTAATGAAAGCAATTGa   <  1:5393615/93‑1 (MQ=255)
                                                                                           |                                                                                         
TCCAGCAATGATACCATCGAGAAATCGATAGCTTCCTGCCAATCTTTTGCTGAATGTATAACTGAGATTGATGATTCTGGAAAATAATCACTAAGCCGCATAAATTATCCTTATTTTATTTTTAGGGTACCGTCCGGCGGTAAAAATAAAATACTCGTGCGGTTTAATGAAAGCAATTGAG  >  NC_000913/3077249‑3077429

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TATCCAGCAATGATACCATCGAGAAATCGATAGCTTCCTGCCAATCTTTTGCTGAATGTATAACTGAGATTGATGATTCTGGAAAATAATCACTAAGCCGCATAAATTATCCTTATTTTATTTTTAGGGTACCGTCCGGCGGTAAAAATAAAATACTCGTGCGGTTTAATGAAAGCAATTGAGCAGG  >  NC_000913/3077247‑3077433
                                                                                             |                                                                                             
TATCCAGCAATGATACCATCGAGAAATCGATAGCTTCCTGCCAATCTTTTGCTGAATGTATAACTGAGATTGATGATTCTGGAAAATAATCACC                                                                                               <  SRR4302542.2885991/94‑1 (MQ=60)
TATCCAGCAATGATACCATCGAGAAATCGATAGCTTCCTGCCAATCTTTTGCTGAATGTATAACTGAGATTGATGATTCTGGAAAATAATCACC                                                                                               <  SRR4302542.1050519/94‑1 (MQ=60)
 ATCCAGCAATGATACCATCGAGAAATCGATAGCTTCCTGCCAATCTTTTGCTGAATGTATAACTGAGATTGATGATTCTGGAAAATAATCACCA                                                                                              <  SRR4302542.424308/94‑1 (MQ=60)
 ATCCAGCAATGATACCATCGAGAAATCGATAGCTTCCTGCCAATCTTTTGCTGAATGTATAACTGAGATTGATGATTCTGGAAAATAATCACCA                                                                                              <  SRR4302542.183483/94‑1 (MQ=60)
  TCCAGCAATGATACCATCGAGAAATCGATAGCTTCCTGCCAATCTTTTGCTGAATGTATAACTGAGATTGATGATTCTGGAAAATAATCACTAA                                                                                             <  SRR4302542.5065685/94‑1 (MQ=60)
     AGCAATGATACCATCGAGAAATCGATAGCTTCCTGCCAATCTTTTGCTGAATGTATAACTGAGATTGATGATTCTGGAAAATAATCACTAAGCC                                                                                          <  SRR4302542.1224852/94‑1 (MQ=60)
     AGCAATGATACCATCGAGAAATCGATAGCTTCCTGCCAATCTTTTGCTGAATGTATAACTGAGATTGATGATTCTGGAAAATAATCACCAAGCC                                                                                          >  SRR4302542.3908227/1‑94 (MQ=60)
       CAATGATACCATCGAGAAATCGATAGCTTCCTGCCAATCTTTTGCTGAATGTATAACTGAGATTGATGATTCTGGAAAATAATCACCAAGCCGC                                                                                        >  SRR4302542.6065990/1‑94 (MQ=60)
        AATGATACCATCGAGAAATCGATAGCTTCCTGCCAATCTTTTGCTGAATGTATAACTGAGATTGATGATTCTGGAAAATAATCACTAAGCCGCA                                                                                       <  SRR4302542.5318133/94‑1 (MQ=60)
        AATGATACCATCGAGAAATCGATAGCTTCCTGCCAATCTTTTGCTGAATGTATAACTGAGATTGATGATTCTGGAAAATAATCACCAAGCCGCA                                                                                       >  SRR4302542.3926148/1‑94 (MQ=60)
         ATGATACCATCGAGAAATCGATAGCTTCCTGCCAATCTTTTGCTGAATGTATAACTGAGATTGATGATTCTGGAAAATAATCACCAAGCCGCAT                                                                                      >  SRR4302542.6062112/1‑94 (MQ=60)
         ATGATACCATCGAGAAATCGATAGCTTCCTGCCAATCTTTTGCTGAATGTATAACTGAGATTGATGATTCTGGAAAATAATCACCAAGCCGCAT                                                                                      <  SRR4302542.3619681/94‑1 (MQ=60)
         ATGATACCATCGAGAAATCGATAGCTTCCTGCCAATCTTTTGCTGAATGTATAACTGAGATTGATGATTCTGGAAAATAATCACCAAGCCGCAT                                                                                      <  SRR4302542.6167589/94‑1 (MQ=60)
           GATACCATCGAGAAATCGATAGCTTCCTGCCAATCTTTTGCTGAATGTATAACTGAGATTGATGATTCTGGAAAATAATCACCAAGCCGCATAA                                                                                    >  SRR4302542.7241880/1‑94 (MQ=60)
           GATACCATCGAGAAATCGATAGCTTCCTGCCAATCTTTTGCTGAATGTATAACTGAGATTGATGATTCTGGAAAATAATCACCAAGCCGCATAA                                                                                    <  SRR4302542.5350477/94‑1 (MQ=60)
            ATACCATCGAGAAATCGATAGCTTCCTGCCAATCTTTTGCTGAATGTATAACTGAGATTGATGATTCTGGAAAATAATCACCAAGCCGCATAAA                                                                                   >  SRR4302542.6378799/1‑94 (MQ=60)
            ATACCATCGAGAAATCGATAGCTTCCTGCCAATCTTTTGCTGAATGTATAACTGAGATTGATGATTCTGGAAAATAATCACCAAGCCGCATAAA                                                                                   <  SRR4302542.7548100/94‑1 (MQ=60)
             TACCATCGAGAAATCGATAGCTTCCTGCCAATCTTTTGCTGAATGTATAACTGAGATTGATGATTCTGGAAAATAATCACCAAGCCGCATAAAT                                                                                  <  SRR4302542.1675595/94‑1 (MQ=60)
             TACCATCGAGAAATCGATAGCTTCCTGCCAATCTTTTGCTGAATGTATAACTGAGATTGATGATTCTGGAAAATAATCACCAAGCCGCATAAAT                                                                                  <  SRR4302542.2807273/94‑1 (MQ=60)
              ACCATCGAGAAATCGATAGCTTCCTGCCAATCTTTTGCTGAATGTATAACTGAGATTGATGATTCTGGAAAATAATCACCAAGCCGCATAAATT                                                                                 >  SRR4302542.4256575/1‑94 (MQ=60)
              ACCATCGAGAAATCGATAGCTTCCTGCCAATCTTTTGCTGAATGTATAACTGAGATTGATGATTCTGGAAAATAATCACCAAGCCGCATAAATT                                                                                 <  SRR4302542.5080112/94‑1 (MQ=60)
              ACCATCGAGAAATCGATAGCTTCCTGCCAATCTTTTGCTGAATGTATAACTGAGATTGATGATTCTGGAAAATAATCACCAAGCCGCATAAATT                                                                                 <  SRR4302542.673624/94‑1 (MQ=60)
               CCATCGAGAAATCGATAGCTTCCTGCCAATCTTTTGCTGAATGTATAACTGAGATTGATGATTCTGGAAAATAATCACCAAGCCGCATAAATTA                                                                                <  SRR4302542.2613662/94‑1 (MQ=60)
                CATCGAGAAATCGATAGCTTCCTGCCAATCTTTTGCTGAATGTATAACTGAGATTGATGATTCTGGAAAATAATCACTAAGCCGCATAAATTAT                                                                               <  SRR4302542.7384563/94‑1 (MQ=60)
                CATCGAGAAATCGATAGCTTCCTGCCAATCTTTTGCTGAATGTATAACTGAGATTGATGATTCTGGAAAATAATCACCAAGCCGCATAAATTAT                                                                               >  SRR4302542.5755915/1‑94 (MQ=60)
                 ATCGAGAAATCGATAGCTTCCTGCCAATCTTTTGCTGAATGTATAACTGAGATTGATGATTCTGGAAAATAATCACCAAGCCGCATAAATTATC                                                                              <  SRR4302542.7177552/94‑1 (MQ=60)
                  TCGAGAAATCGATAGCTTCCTGCCAATCTTTTGCTGAATGTATAACTGAGATTGATGATTCTGGAAAATAATCACCAAGCCGCATAAATTATCC                                                                             <  SRR4302542.4192707/94‑1 (MQ=60)
                   CGAGAAATCGATAGCTTCCTGCCAATCTTTTGCTGAATGTATAACTGAGATTGATGATTCTGGAAAATAATCACCAAGCCGCATAAATTATCCT                                                                            <  SRR4302542.4167761/94‑1 (MQ=60)
                    GAGAAATCGATAGCTTCCTGCCAATCTTTTGCTGAATGTATAACTGAGATTGATGATTCTGGAAAATAATCACCAAGCCGCATAAATTATCCTT                                                                           <  SRR4302542.302410/94‑1 (MQ=60)
                     AGAAATCGATAGCTTCCTGCCAATCTTTTGCTGAATGTATAACTGAGATTGATGATTCTGGAAAATAATCACCAAGCCGCATAAATTATCCTTA                                                                          <  SRR4302542.854985/94‑1 (MQ=60)
                     AGAAATCGATAGCTTCCTGCCAATCTTTTGCTGAATGTATAACTGAGATTGATGATTCTGGAAAATAATCACCAAGCCGCATAAATTATCCTTA                                                                          <  SRR4302542.4778401/94‑1 (MQ=60)
                      GAAATCGATAGCTTCCTGCCAATCTTTTGCTGAATGTATAACTGAGATTGATGATTCTGGAAAATAATCACCAAGCCGCATAAATTATCCTTAT                                                                         <  SRR4302542.4114439/94‑1 (MQ=60)
                        AATCGATAGCTTCCTGCCAATCTTTTGCTGAATGTATAACTGAGATTGATGATTCTGGAAAATAATCACTAAGCCGCATAAATTATCCTTATTT                                                                       <  SRR4302542.2508617/94‑1 (MQ=60)
                        AATCGATAGCTTCCTGCCAATCTTTTGCTGAATGTATAACTGAGATTGATGATTCTGGAAAATAATCACCAAGCCGCATAAATTATCCTTATTT                                                                       <  SRR4302542.7336394/94‑1 (MQ=60)
                        AATCGATAGCTTCCTGCCAATCTTTTGCTGAATGTATAACTGAGATTGATGATTCTGGAAAATAATCACCAAGCCGCATAAATTATCCTTATTT                                                                       <  SRR4302542.579613/94‑1 (MQ=60)
                          TCGATAGCTTCCTGCCAATCTTTTGCTGAATGTATAACTGAGATTGATGATTCTGGAAAATAATCACCAAGCCGCATAAATTATCCTTATTTTA                                                                     <  SRR4302542.2380757/94‑1 (MQ=60)
                          TCGATAGCTTCCTGCCAATCTTTTGCTGAATGTATAACTGAGATTGATGATTCTGGAAAATAATCACCAAGCCGCATAAATTATCCTTATTTTA                                                                     <  SRR4302542.4333033/94‑1 (MQ=60)
                          TCGATAGCTTCCTGCCAATCTTTTGCTGAATGTATAACTGAGATTGATGATTCTGGAAAATAATCACCAAGCCGCATAAATTATCCTTATTTTA                                                                     <  SRR4302542.2590164/94‑1 (MQ=60)
                          TCGATAGCTTCCTGCCAATCTTTTGCTGAATGTATAACTGAGATTGATGATTCTGGAAAATAATCACCAAGCCGCATAAATTATCCTTATTTTA                                                                     <  SRR4302542.6254366/94‑1 (MQ=60)
                           CGATAGCTTCCTGCCAATCTTTTGCTGAATGTATAACTGAGATTGATGATTCTGGAAAATAATCCCCAAGCCGCATAAATTATCCTTATTTTAT                                                                    <  SRR4302542.4595091/94‑1 (MQ=60)
                           CGATAGCTTCCTGCCAATCTTTTGCTGAATGTATAACTGAGATTGATGATTCTGGAAAATAATCACTAAGCCGCATAAATTATCCTTATTTTAT                                                                    <  SRR4302542.991227/94‑1 (MQ=60)
                           CGATAGCTTCCTGCCAATCTTTTGCTGAATGTATAACTGAGATTGATGATTCTGGAAAATAATCACCAAGCCGCATAAATTATCCTTATTTTAT                                                                    <  SRR4302542.2733271/94‑1 (MQ=60)
                           CGATAGCTTCCTGCCAATCTTTTGCTGAATGTATAACTGAGATTGATGATTCTGGAAAATAATCACCAAGCCGCATAAATTATCCTTATTTTAT                                                                    >  SRR4302542.4235563/1‑94 (MQ=60)
                            GATAGCTTCCTGCCAATCTTTTGCTGAATGTATAACTGAGATTGATGATTCTGGAAAATAATCACCAAGCCGCATAAATTATCCTTATTTTATT                                                                   <  SRR4302542.4704901/94‑1 (MQ=60)
                            GATAGCTTCCTGCCAATCTTTTGCTGAATGTATAACTGAGATTGATGATTCTGGAAAATAATCACCAAGCCGCATAAATTATCCTTATTTTATT                                                                   >  SRR4302542.4564873/1‑94 (MQ=60)
                            GATAGCTTCCTGCCAATCTTTTGCTGAATGTATAACTGAGATTGATGATTCTGGAAAATAATCACCAAGCCGCATAAATTATCCTTATTTTATT                                                                   <  SRR4302542.1385633/94‑1 (MQ=60)
                             ATAGCTTCCTGCCAATCTTTTGCTGAATGTATAACTGAGATTGATGATTCTGGAAAATAATCACCAAGCCGCATAAATTATCCTTATTTTATTT                                                                  <  SRR4302542.2041161/94‑1 (MQ=60)
                             ATAGCTTCCTGCCAATCTTTTGCTGAATGTATAACTGAGATTGATGATTCTGGAAAATAATCACCAAGCCGCATAAATTATCCTTATTTTATTT                                                                  <  SRR4302542.5519246/94‑1 (MQ=60)
                             ATAGCTTCCTGCCAATCTTTTGCTGAATGTATAACTGAGATTGATGATTCTGGAAAATAATCACCAAGCCGCATAAATTATCCTTATTTTATTT                                                                  <  SRR4302542.1414846/94‑1 (MQ=60)
                              TAGCTTCCTGCCAATCTTTTGCTGAATGTATAACTGAGATTGATGATTCTGGAAAATAATCACCAAGCCGCATAAATTATCCTTATTTTATTTT                                                                 <  SRR4302542.4718455/94‑1 (MQ=60)
                              TAGCTTCCTGCCAATCTTTTGCTGAATGTATAACTGAGATTGATGATTCTGGAAAATAATCACCAAGCCGCATAAATTATCCTTATTTTATTTT                                                                 <  SRR4302542.3277244/94‑1 (MQ=60)
                              TAGCTTCCTGCCAATCTTTTGCTGAATGTATAACTGAGATTGATGATTCTGGAAAATAATCACCAAGCCGCATAAATTATCCTTATTTTATTTT                                                                 <  SRR4302542.3385282/94‑1 (MQ=60)
                               AGCTTCCTGCCAATCTTTTGCTGAATGTATAACTGAGATTGATGATTCTGGAAAATAATCACCAAGCCGCATAAATTATCCTTATTTTATTTTT                                                                <  SRR4302542.459829/94‑1 (MQ=60)
                               AGCTTCCTGCCAATCTTTTGCTGAATGTATAACTGAGATTGATGATTCTGGAAAATAATCACCAAGCCGCATAAATTATCCTTATTTTATTTTT                                                                >  SRR4302542.1805255/1‑94 (MQ=60)
                               AGCTTCCTGCCAATCTTTTGCTGAATGTATAACTGAGATTGATGATTCTGGAAAATAATCACCAAGCCGCATAAATTATCCTTATTTTATTTTT                                                                >  SRR4302542.4077298/1‑94 (MQ=60)
                                  TTCCTGCCAATCTTTTGCTGAATGTATAACTGAGATTGATGATTCTGGAAAATAATCACCAAGCCGCATAAATTATCCTTATTTTATTTTTAGG                                                             >  SRR4302542.3693086/1‑94 (MQ=60)
                                   TCCTGCCAATCTTTTGCTGAATGTATAACTGAGATTGATGATTCTGGAAAATAATCACCAAGCCGCATAAATTATCCTTATTTTATTTTTAGGG                                                            <  SRR4302542.6720007/94‑1 (MQ=60)
                                    CCTGCCAATCTTTTGCTGAATGTATAACTGAGATTGATGATTCTGGAAAATAATCACCAAGCCGCATAAATTATCCTTATTTTATTTTTAGGGT                                                           <  SRR4302542.3433221/94‑1 (MQ=60)
                                    CCTGCCAATCTTTTGCTGAATGTATAACTGAGATTGATGATTCTGGAAAATAATCACCAAGCCGCATAAATTATCCTTATTTTATTTTTAGGGT                                                           <  SRR4302542.3970734/94‑1 (MQ=60)
                                     CTGCCAATCTTTTGCTGAATGTATAACTGAGATTGATGATTCTGGAAAATAATCACCAAGCCGCATAAATTATCCTTATTTTATTTTTAGGGTA                                                          >  SRR4302542.6702559/1‑94 (MQ=60)
                                     CTGCCAATCTTTTGCTGAATGTATAACTGAGATTGATGATTCTGGAAAATAATCACCAAGCCGCATAAATTATCCTTATTTTATTTTTAGGGTA                                                          <  SRR4302542.476402/94‑1 (MQ=60)
                                     CTGCCAATCTTTTGCTGAATGTATAACTGAGATTGATGATTCTGGAAAATAATCACCAAGCCGCATAAATTATCCTTATTTTATTTTTAGGGTA                                                          >  SRR4302542.409243/1‑94 (MQ=60)
                                      TGCCAATCTTTTGCTGAATGTATAACTGAGATTGATGATTCTGGAAAATAATCACCAAGCCGCATAAATTATCCTTATTTTATTTTTAGGGTAC                                                         >  SRR4302542.3317333/1‑94 (MQ=60)
                                       GCCAATCTTTTGCTGAATGTATAACTGAGATTGATGATTCTGGAAAATAATCACTAAGCCGCATAAATTATCCTTATTTTATTTTTAGGGTACC                                                        >  SRR4302542.4014179/1‑94 (MQ=60)
                                       GCCAATCTTTTGCTGAATGTATAACTGAGATTGATGATTCTGGAAAATAATCACCAAGCCGCATAAATTATCCTTATTTTATTTTTAGGGTACC                                                        <  SRR4302542.880852/94‑1 (MQ=60)
                                       GCCAATCTTTTGCTGAATGTATAACTGAGATTGATGATTCTGGAAAATAATCACCAAGCCGCATAAATTATCCTTATTTTATTTTTAGGGTACC                                                        <  SRR4302542.6723118/94‑1 (MQ=60)
                                       GCCAATCTTTTGCTGAATGTATAACTGAGATTGATGATTCTGGAAAATAATCACCAAGCCGCATAAATTATCCTTATTTTATTTTTAGGGTACC                                                        <  SRR4302542.4880142/94‑1 (MQ=60)
                                       GCCAATCTTTTGCTGAATGTATAACTGAGATTGATGATTCTGGAAAATAATCACCAAGCCGCATAAATTATCCTTATTTTATTTTTAGGGTACC                                                        <  SRR4302542.590512/94‑1 (MQ=60)
                                        CCAATCTTTTGCTGAATGTATAACTGAGATTGATGATTCTGGAAAATAATCACCAAGCCGCATAAATTATCCTTATTTTATTTTTAGGGTACCG                                                       <  SRR4302542.1388807/94‑1 (MQ=60)
                                         CAATCTTTTGCTGAATGTATAACTGAGATTGATGATTCTGGAAAATAATCACCAAGCCGCATAAATTATCCTTATTTTATTTTTAGGGTACCGT                                                      <  SRR4302542.770438/94‑1 (MQ=60)
                                          AATCTTTTGCTGAATGTATAACTGAGATTGATGATTCTGGAAAATAATCACTAAGCCGCATAAATTATCCTTATTTTATTTTTAGGGTACCGTC                                                     <  SRR4302542.6458307/94‑1 (MQ=60)
                                           ATCTTTTGCTGAATGTATAACTGAGATTGATGATTCTGGAAAATAATCACTAAGCCGCATAAATTATCCTTATTTTATTTTTAGGGTACCGTCC                                                    <  SRR4302542.4963307/94‑1 (MQ=60)
                                             CTTTTGCTGAATGTATAACTGAGATTGATGATTCTGGAAAATAATCACCAAGCCGCATAAATTATCCTTATTTTATTTTTAGGGTACCGTCCGG                                                  >  SRR4302542.6750441/1‑94 (MQ=60)
                                              TTTTGCTGAATGTATAACTGAGATTGATGATTCTGGAAAATAATCACCAAGCCGCATAAATTATCCTTATTTTATTTTTAGGGTACCGTCCGGC                                                 <  SRR4302542.4209787/94‑1 (MQ=60)
                                                TTGCTGAATGTATAACTGAGATTGATGATTCTGGAAAATAATCACTAAGCCGCATAAATTATCCTTATTTTATTTTTAGGGTACCGTCCGGCGG                                               >  SRR4302542.4012712/1‑94 (MQ=60)
                                                  GCTGAATGTATAACTGAGATTGATGATTCTGGAAAATAATCACCAAGCCGCATAAATTATCCTTATTTTATTTTTAGGGTACCGTCCGGCGGTA                                             <  SRR4302542.2937515/94‑1 (MQ=60)
                                                   CTGAATGTATAACTGAGATTGATGATTCTGGAAAATAATCACTAAGCCGCATAAATTATCCTTATTTTATTTTTAGGGTACCGTCCGGCGGTAA                                            >  SRR4302542.4837074/1‑94 (MQ=60)
                                                   CTGAATGTATAACTGAGATTGATGATTCTGGAAAATAATCACCAAGCCGCATAAATTATCCTTATTTTATTTTTAGGGTACCGTCCGGCGGTAA                                            >  SRR4302542.5509372/1‑94 (MQ=60)
                                                     GAATGTATAACTGAGATTGATGATTCTGGAAAATAATCACCAAGCCGCATAAATTATCCTTATTTTATTTTTAGGGTACCGTCCGGCGGTAAAA                                          >  SRR4302542.6358142/1‑94 (MQ=60)
                                                       ATGTATAACTGAGATTGATGATTCTGGAAAATAATCACCAAGCCGCATAAATTATCCTTATTTTATTTTTAGGGTACCGTCCGGCGGTAAAAAT                                        >  SRR4302542.6477106/1‑94 (MQ=60)
                                                         GTATAACTGAGATTGATGATTCTGGAAAATAATCACCAAGCCGCATAAATTATCCTTATTTTATTTTTAGGGTACCGTCCGGCGGTAAAAATAA                                      <  SRR4302542.6574579/94‑1 (MQ=60)
                                                         GTATAACTGAGATTGATGATTCTGGAAAATAATCACCAAGCCGCATAAATTATCCTTATTTTATTTTTAGGGTACCGTCCGGCGGTAAAAATAA                                      <  SRR4302542.606721/94‑1 (MQ=60)
                                                          TATAACTGAGATTGATGATTCTGGAAAATAATCACCAAGCCGCATAAATTATCCTTATTTTATTTTTAGGGTACCGTCCGGCGGTAAAAATAAA                                     >  SRR4302542.3753520/1‑94 (MQ=60)
                                                            TAACTGAGATTGATGATTCTGGAAAATAATCACCAAGCCGCATAAATTATCCTTATTTTATTTTTAGGGTACCGTCCGGCGGTAAAAATAAAAT                                   <  SRR4302542.54697/94‑1 (MQ=60)
                                                              ACTGAGATTGATGATTCTGGAAAATAATCACCAAGCCGCATAAATTATCCTTATTTTATTTTTAGGGTACCGTCCGGCGGTAAAAATAAAATAC                                 <  SRR4302542.5620850/94‑1 (MQ=60)
                                                              ACTGAGATTGATGATTCTGGAAAATAATCACCAAGCCGCATAAATTATCCTTATTTTATTTTTAGGGTACCGTCCGGCGGTAAAAATAAAATAC                                 <  SRR4302542.6377276/94‑1 (MQ=60)
                                                               CTGAGATTGATGATTCTGGAAAATAATCACCAAGCCGCATAAATTATCCTTATTTTATTTTTAGGGTACCGTCCGGCGGTAAAAATAAAATACT                                >  SRR4302542.5306152/1‑94 (MQ=60)
                                                                TGAGATTGATGATTCTGGAAAATAATCACTAAGCCGCATAAATTATCCTTATTTTATTTTTAGGGTACCGTCCGGCGGTAAAAATAAAATACTC                               <  SRR4302542.3518248/94‑1 (MQ=60)
                                                                TGAGATTGATGATTCTGGAAAATAATCACCAAGCCGCATAAATTATCCTTATTTTATTTTTAGGGTACCGTCCGGCGGTAAAAATAAAATACTC                               <  SRR4302542.4495932/94‑1 (MQ=60)
                                                                 GAGATTGATGATTCTGGAAAATAATCACCAAGCCGCATAAATTATCCTTATTTTATTTTTAGGGTACCGTCCGGCGGTAAAAATAAAATACTCG                              <  SRR4302542.6028471/94‑1 (MQ=60)
                                                                 GAGATTGATGATTCTGGAAAATAATCACCAAGCCGCATAAATTATCCTTATTTTATTTTTAGGGTACCGTCCGGCGGTAAAAATAAAATACTCG                              <  SRR4302542.6817550/94‑1 (MQ=60)
                                                                  AGATTGATGATTCTGGAAAATAATCACTAAGCCGCATAAATTATCCTTATTTTATTTTTAGGGTACCGTCCGGCGGTAAAAATAAAATACTCGT                             <  SRR4302542.6793255/94‑1 (MQ=60)
                                                                   GATTGATGATTCTGGAAAATAATCACTAAGCCGCATAAATTATCCTTATTTTATTTTTAGGGTACCGTCCGGCGGTAAAAATAAAATACTCGTG                            >  SRR4302542.6249509/1‑94 (MQ=60)
                                                                    ATTGATGATTCTGGAAAATAATCACCAAGCCGCATAAATTATCCTTATTTTATTTTTAGGGTACCGTCCGGCGGTAAAAATAAAATACTCGTGC                           >  SRR4302542.3398569/1‑94 (MQ=60)
                                                                    ATTGATGATTCTGGAAAATAATCACCAAGCCGCATAAATTATCCTTATTTTATTTTTAGGGTACCGTCCGGCGGTAAAAATAAAATACTCGTGC                           <  SRR4302542.295426/94‑1 (MQ=60)
                                                                      TGATGATTCTGGAAAATAATCACTAAGCCGCATAAATTATCCTTATTTTATTTTTAGGGTACCGTCCGGCGGTAAAAATAAAATACTCGTGCGG                         >  SRR4302542.5605326/1‑94 (MQ=60)
                                                                         TGATTCTGGAAAATAATCACCAAGCCGCATAAATTATCCTTATTTTATTTTTAGGGTACCGTCCGGCGGTAAAAATAAAATACTCGTGCGGTTT                      <  SRR4302542.3816065/94‑1 (MQ=60)
                                                                          GATTCTGGAAAATAATCACCAAGCCGCATAAATTATCCTTATTTTATTTTTAGGGTACCGTCCGGCGGTAAAAATAAAATACTCGTGCGGTTTA                     >  SRR4302542.15830/1‑94 (MQ=60)
                                                                             TCTGGAAAATAATCACCAAGCCGCATAAATTATCCTTATTTTATTTTTAGGGTACCGTCCGGCGGTAAAAATAAAATACTCGTGCGGTTTAATG                  <  SRR4302542.1164718/94‑1 (MQ=60)
                                                                              CTGGAAAATAATCACCAAGCCGCATAAATTATCCTTATTTTATTTTTAGGGTACCGTCCGGCGGTAAAAATAAAATACTCGTGCGGTTTAATGA                 <  SRR4302542.6814980/94‑1 (MQ=60)
                                                                               TGGAAAATAATCACCAAGCCGCATAAATTATCCTTATTTTATTTTTAGGGTACCGTCCGGCGGTAAAAATAAAATACTCGTGCGGTTTAATGAA                <  SRR4302542.3862326/94‑1 (MQ=60)
                                                                                GGAAAATAATCACCAAGCCGCATAAATTATCCTTATTTTATTTTTAGGGTACCGTCCGGCGGTAAAAATAAAATACTCGTGCGGTTTAATGAAA               <  SRR4302542.1924870/94‑1 (MQ=60)
                                                                                 GAAAATAATCACTAAGCCGCATAAATTATCCTTATTTTATTTTTAGGGTACCGTCCGGCGGTAAAAATAAAATACTCGTGCGGTTTAATGAAAG              <  SRR4302542.4985443/94‑1 (MQ=60)
                                                                                 GAAAATAATCACCAAGCCGCATAAATTATCCTTATTTTATTTTTAGGGTACCGTCCGGCGGTAAAAATAAAATACTCGTGCGGTTTAATGAAAG              <  SRR4302542.224526/94‑1 (MQ=60)
                                                                                 GAAAATAATCACCAAGCCGCATAAATTATCCTTATTTTATTTTTAGGGTACCGTCCGGCGGTAAAAATAAAATACTCGTGCGGTTTAATGAAAG              >  SRR4302542.3073115/1‑94 (MQ=60)
                                                                                 GAAAATAATCACCAAGCCGCATAAATTATCCTTATTTTATTTTTAGGGTACCGTCCGGCGGTAAAAATAAAATACTCGTGCGGTTTAATGAAAG              <  SRR4302542.5545473/94‑1 (MQ=60)
                                                                                 GAAAATAATCACCAAGCCGCATAAATTATCCTTATTTTATTTTTAGGGTACCGTCCGGCGGTAAAAATAAAATACTCGTGCGGTTTAATGAAAG              <  SRR4302542.1758743/94‑1 (MQ=60)
                                                                                     ATAATCACCAAGCCGCATAAATTATCCTTATTTTATTTTTAGGGTACCGTCCGGCGGTAAAAATAAAATACTCGTGCGGTTTAATGAAAGCAAT          <  SRR4302542.4495656/94‑1 (MQ=60)
                                                                                     ATAATCACCAAGCCGCATAAATTATCCTTATTTTATTTTTAGGGTACCGTCCGGCGGTAAAAATAAAATACTCGTGCGGTTTAATGAAAGCAAT          >  SRR4302542.6994473/1‑94 (MQ=60)
                                                                                       AATCACCAAGCCGCATAAATTATCCTTATTTTATTTTTAGGGTACCGTCCGGCGGTAAAAATAAAATACTCGTGCGGTTTAATGAAAGCAATTG        <  SRR4302542.4700404/94‑1 (MQ=60)
                                                                                         TCACCAAGCCGCATAAATTATCCTTATTTTATTTTTAGGGTACCGTCCGGCGGTAAAAATAAAATACTCGTGCGGTTTAATGAAAGCAATTGAG      <  SRR4302542.5446002/94‑1 (MQ=60)
                                                                                          CACTAAGCCGCATAAATTATCCTTATTTTATTTTTAGGGTACCGTCCGGCGGTAAAAATAAAATACTCGTGCGGTTTAATGAAAGCAATTGAGC     <  SRR4302542.5477551/94‑1 (MQ=60)
                                                                                          CACCAAGCCGCATAAATTATCCTTATTTTATTTTTAGGGTACCGTCCGGCGGTAAAAATAAAATACTCGTGCGGTTTAATGAAAGCAATTGAGC     <  SRR4302542.3020238/94‑1 (MQ=60)
                                                                                           ACCAAGCCGCATAAATTATCCTTATTTTATTTTTAGGGTACCGTCCGGCGGTAAAAATAAAATACTCGTGCGGTTTAATGAAAGCAATTGAGCA    <  SRR4302542.138292/94‑1 (MQ=60)
                                                                                            CCAAGCCGCATAAATTATCCTTATTTTATTTTTAGGGTACCGTCCGGCGGTAAAAATAAAATACTCGTGCGGTTTAATGAAAGCAATTGAGCAG   >  SRR4302542.4893506/1‑94 (MQ=60)
                                                                                             CAAGCCGCATAAATTATCCTTATTTTATTTTTAGGGTACCGTCCGGCGGTAAAAATAAAATACTCGTGCGGTTTAATGAAAGCAATTGAGCAGG  <  SRR4302542.2250396/94‑1 (MQ=60)
                                                                                             |                                                                                             
TATCCAGCAATGATACCATCGAGAAATCGATAGCTTCCTGCCAATCTTTTGCTGAATGTATAACTGAGATTGATGATTCTGGAAAATAATCACTAAGCCGCATAAATTATCCTTATTTTATTTTTAGGGTACCGTCCGGCGGTAAAAATAAAATACTCGTGCGGTTTAATGAAAGCAATTGAGCAGG  >  NC_000913/3077247‑3077433

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑