Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	1061	294.1	15065038	98.6%	14854127	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,077,640	+G	intergenic (‑291/+23)	cmtB ← / ← tktA	PTS system mannitol‑specific enzyme IIA component/transketolase 1 isozyme

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,077,640	1	.	G	99.5%	636.6 / ‑4.1	188	intergenic (‑291/+23)	cmtB/tktA	PTS system mannitol‑specific enzyme IIA component/transketolase 1 isozyme
Reads supporting (aligned to +/- strand): ref base . (1/0); major base G (141/45); minor base T (0/1); total (142/46)
Fisher's exact test for biased strand distribution p-value = 2.46e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.90e-01

Only 100 of 199 total aligned reads displayed.

TATTGCATATAAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCC‑GAAGC‑ACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGCCGAACTCTTCAAACAGC  >  NC_000913/3077548‑3077726

tattGCATATAAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCC‑GAAGc                                                                                         >  2:7032735/1‑93 (MQ=255)

  ttGCATATAAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCC‑GAAGcga                                                                                       <  1:5247937/93‑3 (MQ=255)

    gCATATAAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCC‑GAAGcgacc                                                                                     <  2:5456370/93‑5 (MQ=255)

      atatAAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCC‑GAAGCGACCtt                                                                                   <  1:3923968/93‑1 (MQ=255)

      atatAAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCC‑GAAGCGACCtt                                                                                   >  2:3476627/1‑93 (MQ=255)

       tataAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCC‑GAAGCGACCttt                                                                                  >  1:4552255/1‑93 (MQ=255)

       tataAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCC‑GAAGCGACCttt                                                                                  >  1:882537/1‑93 (MQ=255)

       tataAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCC‑GAAGCGACCttt                                                                                  >  2:1599698/1‑93 (MQ=255)

       tataAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCC‑GAAGCGACCttt                                                                                  >  1:4638702/1‑93 (MQ=255)

       tataAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCC‑GAAGCGACCttt                                                                                  >  2:2360738/1‑93 (MQ=255)

       tataAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCC‑GAAGCGACCttt                                                                                  >  1:3313548/1‑93 (MQ=255)

       tataAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCC‑GAAGCGACCttt                                                                                  >  1:2734563/1‑93 (MQ=255)

        ataAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCC‑GAAGCGACCtttt                                                                                 >  1:6242207/1‑93 (MQ=255)

         taAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCC‑GAAGCGACCttttt                                                                                >  2:2762452/1‑93 (MQ=255)

           aaTATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCC‑GAAGCGACCTTTTTAc                                                                              >  2:3287638/1‑93 (MQ=255)

           aaTATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCC‑GAAGCGACCTTTTTAc                                                                              >  1:3246795/1‑93 (MQ=255)

            atatTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCC‑GAAGCGACCTTTTTAcc                                                                             >  1:5980106/1‑93 (MQ=255)

            atatTTGTGATCTACAACACGCCTCATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCC‑GAAGCGACCTTTTTAcc                                                                             >  2:619667/1‑93 (MQ=255)

             tatTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCC‑GAAGCGACCTTTTTAccc                                                                            >  2:2024552/1‑93 (MQ=255)

               tttGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCC‑GAAGCGACCTTTTTACCCGa                                                                          >  1:830691/1‑93 (MQ=255)

               tttGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCC‑GAAGCGACCTTTTTACCCGa                                                                          >  2:2909074/1‑93 (MQ=255)

                 tgtgATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCC‑GAAGCGACCTTTTTACCCGaaa                                                                        <  1:1517699/93‑1 (MQ=255)

                    gATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCC‑GAAGCGACCTTTTTACCCGAAATGc                                                                     >  1:4837192/1‑93 (MQ=255)

                     aTCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCC‑GAAGCGACCTTTTTACCCGAAATGCt                                                                    >  1:2185330/1‑93 (MQ=255)

                      tCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCC‑GAAGCGACCTTTTTACCCGAAATGCTa                                                                   >  1:7409136/1‑93 (MQ=255)

                       cTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCC‑GAAGCGACCTTTTTACCCGAAATGCTaa                                                                  <  1:4892816/93‑1 (MQ=255)

                        tACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCC‑GAAGCGACCTTTTTACCCGAAATGCTAAt                                                                 >  1:5569793/1‑93 (MQ=255)

                        tACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCC‑GAAGCGACCTTTTTACCCGAAATGCTAAt                                                                 >  2:1327379/1‑93 (MQ=255)

                         acaacaCGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCC‑GAAGCGACCTTTTTACCCGAAATGCTAAtt                                                                >  1:307691/1‑93 (MQ=255)

                         acaacaCGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCC‑GAAGCGACCTTTTTACCCGAAATGCTAAtt                                                                >  2:4958465/1‑93 (MQ=255)

                          caacaCGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCC‑GAAGCGACCTTTTTACCCGAAATGCTAATTa                                                               >  2:7445039/1‑93 (MQ=255)

                           aacaCGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCC‑GAAGCGACCTTTTTACCCGAAATGCTAATTAc                                                              >  2:1671812/1‑93 (MQ=255)

                           aacaCGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCC‑GAAGCGACCTTTTTACCCGAAATGCTAATTAc                                                              <  1:2960138/93‑1 (MQ=255)

                           aacaCGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCC‑GAAGCGACCTTTTTACCCGAAATGCTAATTAc                                                              >  2:4063316/1‑93 (MQ=255)

                            acacGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCC‑GAAGCGACCTTTTTACCCGAAATGCTAATTAca                                                             >  1:2194618/1‑93 (MQ=255)

                            acacGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCC‑GAAGCGACCTTTTTACCCGAAATGCTAATTAca                                                             >  1:2060886/1‑93 (MQ=255)

                             cacGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCC‑GAAGCGACCTTTTTACCCGAAATGCTAATTAcag                                                            <  1:5883991/93‑1 (MQ=255)

                             cacGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCC‑GAAGCGACCTTTTTACCCGAAATGCTAATTAcag                                                            >  1:115941/1‑93 (MQ=255)

                               cGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGGAAGG‑ACCCTTTTTCCCGGAATGGTAATTTcaaga                                                          >  1:6816752/1‑90 (MQ=255)

                               cGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCC‑GAAGCGACCTTTTTACCCGAAATGCTAATTAcagca                                                          <  2:3094936/93‑1 (MQ=255)

                               cGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCC‑GAAGCGACCTTTTTACCCGAAATGCTAATTAcagca                                                          <  2:6286867/93‑1 (MQ=255)

                               cGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCC‑GAAGCGACCTTTTTACCCGAAATGCTAATTAcagca                                                          >  2:6483033/1‑93 (MQ=255)

                                   ttATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCC‑GAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTc                                                      >  1:1476861/1‑93 (MQ=255)

                                      tCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCC‑GAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCttt                                                   >  2:4820504/1‑93 (MQ=255)

                                      tCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCC‑GAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCttt                                                   >  1:796063/1‑93 (MQ=255)

                                       cTATTGCTTGTCCGCAAACGGACATGTCAAGGTAATAAAAAAGGTCGCC‑GAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCtttt                                                  >  1:7222104/1‑93 (MQ=255)

                                         aTTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCC‑GAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGc                                                >  2:1512522/1‑93 (MQ=255)

                                         aTTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCC‑GAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGc                                                >  1:650617/1‑93 (MQ=255)

                                           tGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCC‑GAAGCTACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCtt                                              <  2:3716754/93‑1 (MQ=255)

                                           tGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCC‑GAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCtt                                              <  2:3012397/93‑1 (MQ=255)

                                           tGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCC‑GAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCtt                                              <  2:5564140/93‑1 (MQ=255)

                                            gCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCC‑GAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCttt                                             >  1:7101801/1‑93 (MQ=255)

                                            gCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCC‑GAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCttt                                             >  1:6614299/1‑93 (MQ=255)

                                            gCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCC‑GAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCttt                                             <  2:4466850/93‑1 (MQ=255)

                                             cTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCC‑GAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTc                                            >  1:1515021/1‑93 (MQ=255)

                                              ttGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCC‑GAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCg                                           >  1:4720396/1‑93 (MQ=255)

                                              ttGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCC‑GAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCg                                           >  1:2582646/1‑93 (MQ=255)

                                              ttGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCC‑GAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCg                                           >  1:4354438/1‑93 (MQ=255)

                                                gTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCC‑GAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGca                                         >  2:6820123/1‑93 (MQ=255)

                                                 tCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCC‑GAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGcaa                                        >  1:476658/1‑93 (MQ=255)

                                                 tCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCC‑GAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGcaa                                        >  1:7320722/1‑93 (MQ=255)

                                                    gCAAACGGACATATCAAGGTAATAAAAAAGGTCGCC‑GAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGcaacaa                                     >  2:4376973/1‑93 (MQ=255)

                                                    gCAAACGGACATATCAAGGTAATAAAAAAGGTCGCC‑GAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGcaacaa                                     >  1:3562293/1‑93 (MQ=255)

                                                     cAAACGGACATATCAAGGTAATAAAAAAGGTCGCC‑GAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCaacaac                                    >  1:5818076/1‑93 (MQ=255)

                                                       aaCGGACATATCAAGGTAATAAAAAAGGTCGCC‑GAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGt                                  <  1:4075049/93‑1 (MQ=255)

                                                       aaCGGACATATCAAGGTAATAAAAAAGGTCGCC‑GAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGt                                  >  2:5354682/1‑93 (MQ=255)

                                                         cGGACATATCAAGGTAATAAAAAAGGTCGCC‑GAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTa                                <  2:4928148/93‑1 (MQ=255)

                                                          ggACATATCAAGGTAATAAAAAAGGTCGCC‑GAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTAt                               <  1:4564277/93‑1 (MQ=255)

                                                              atatCAAGGTAATAAAAAAGGTCGCC‑GAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCaca                           >  1:5036053/1‑91 (MQ=255)

                                                               tatCAAGGTAATAAAAAAGGTCGCC‑GAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACa                          >  1:4919252/1‑93 (MQ=255)

                                                               tatCAAGGTAATAAAAAAGGTCGCC‑GAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACa                          >  1:415144/1‑93 (MQ=255)

                                                                 tCAAGGTAATAAAAAAGGTCGCC‑GAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGt                        >  2:69335/1‑93 (MQ=255)

                                                                    aGGTAATAAAAAAGGTCGCC‑GAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGaa                     >  2:2181539/1‑93 (MQ=255)

                                                                    aGGTAATAAAAAAGGTCGCC‑GAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGaa                     >  1:1283714/1‑93 (MQ=255)

                                                                    aGGTAATAAAAAAGGTCGCC‑GAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGaa                     >  2:81261/1‑93 (MQ=255)

                                                                    aGGGAATAAAAAAGGTCGCC‑GAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGaa                     >  1:6974754/1‑93 (MQ=255)

                                                                     ggTAATAAAAAAGGTCGCC‑GAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAg                    >  2:948994/1‑93 (MQ=255)

                                                                     ggTAATAAAAAAGGTCGCC‑GAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAg                    >  1:5851524/1‑93 (MQ=255)

                                                                     ggTAATAAAAAAGGTCGCC‑GAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAg                    >  2:5198643/1‑93 (MQ=255)

                                                                      gTAATAAAAAAGGTCGCC‑GAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGc                   <  2:4710741/93‑1 (MQ=255)

                                                                      gTAATAAAAAAGGTCGCC‑GAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGc                   >  2:7182790/1‑93 (MQ=255)

                                                                      gTAATAAAAAAGGTCGCC‑GAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGc                   >  2:1125537/1‑93 (MQ=255)

                                                                      gTAATAAAAAAGGTCGCC‑GAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGc                   <  1:340694/93‑1 (MQ=255)

                                                                       taataaAAAAGGTCGCC‑GAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGcc                  <  2:5052042/93‑1 (MQ=255)

                                                                         ataaAAAAGGTCGCC‑GAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGCCGa                >  1:5342634/1‑93 (MQ=255)

                                                                           aaaaaaGGTCGCC‑GAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGCCGAAc              <  1:7465153/93‑1 (MQ=255)

                                                                            aaaaaGGTCGCC‑GAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGCCGAAct             >  2:3945126/1‑93 (MQ=255)

                                                                            aaaaaGGTCGCC‑GAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGCCGAAct             >  1:269740/1‑93 (MQ=255)

                                                                            aaaaaGGTCGCC‑GAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGCCGAAct             >  1:2770493/1‑93 (MQ=255)

                                                                             aaaaGGTCGCC‑GAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGCCGAActc            >  2:417515/1‑93 (MQ=255)

                                                                              aaaGGTCGCC‑GAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGCCGAActct           >  2:6471161/1‑93 (MQ=255)

                                                                              aaaGGTCGCC‑GAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGCCGAActct           <  2:1426305/93‑1 (MQ=255)

                                                                                aGGTCGCC‑GAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGCCGAACTCTTc         >  2:5253933/1‑93 (MQ=255)

                                                                                aGGTCGCC‑GAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGCCGAACTCTTc         >  2:2875580/1‑93 (MQ=255)

                                                                                aGGTCGCC‑GAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGCCGAACTCTTc         >  1:3901955/1‑93 (MQ=255)

                                                                                  gTCGCC‑GAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGCCGAACTCTTCaa       <  1:3668625/93‑1 (MQ=255)

                                                                                     gCC‑GAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGCCGAACTCTTCAAAca    >  1:2459810/1‑93 (MQ=255)

                                                                                       c‑GAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGCCGAACTCTTCAAAcagc  <  2:5807524/93‑1 (MQ=255)

                                                                                       c‑GAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGCCGAACTCTTCAAAcagc  >  2:5036216/1‑93 (MQ=255)

                                                                                       c‑GAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGCCGAACTCTTCAAAcagc  <  1:1047485/93‑1 (MQ=255)

TATTGCATATAAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCC‑GAAGC‑ACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGCCGAACTCTTCAAACAGC  >  NC_000913/3077548‑3077726

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

TTATTGCATATAAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGC‑ACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGCCGAACTCTTCAAACAGCAGCTCTG  >  NC_000913/3077547‑3077733

TTATTGCATATAAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGC                                                                                                >  SRR4302542.58932/1‑94 (MQ=60)

TTATTGCATATAAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGC                                                                                                >  SRR4302542.2212310/1‑94 (MQ=60)

TTATTGCATATAAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGC                                                                                                >  SRR4302542.4602245/1‑94 (MQ=60)

 TATTGCATATAAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGC‑G                                                                                              >  SRR4302542.5790437/1‑94 (MQ=60)

  ATTGCATATAAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCga                                                                                              >  SRR4302542.406345/1‑92 (MQ=60)

   TTGCATATAAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCgac                                                                                             <  SRR4302542.5298949/94‑4 (MQ=60)

    TGCATATAAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTGATAAAAAAGGTCGCCGAAGCGACC                                                                                            >  SRR4302542.6977542/1‑94 (MQ=60)

    TGCATATAAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACC                                                                                            >  SRR4302542.2782640/1‑94 (MQ=60)

     GCATATAAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCT                                                                                           >  SRR4302542.154546/1‑94 (MQ=60)

     GCATATAAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCT                                                                                           >  SRR4302542.1924657/1‑94 (MQ=60)

      CATATAAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTT                                                                                          <  SRR4302542.2364828/94‑1 (MQ=60)

       ATATAAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTT                                                                                         >  SRR4302542.4596803/1‑94 (MQ=60)

       ATATAAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTT                                                                                         <  SRR4302542.3962689/94‑1 (MQ=60)

       ATATAAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTT                                                                                         >  SRR4302542.378382/1‑94 (MQ=60)

       ATATAAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTT                                                                                         >  SRR4302542.3344779/1‑94 (MQ=60)

       ATATAAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTT                                                                                         >  SRR4302542.4684025/1‑94 (MQ=60)

       ATATAAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTT                                                                                         >  SRR4302542.2760939/1‑94 (MQ=60)

       ATATAAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTT                                                                                         <  SRR4302542.2569712/94‑1 (MQ=60)

       ATATAAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTT                                                                                         >  SRR4302542.890166/1‑94 (MQ=60)

        TATAAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTT                                                                                        >  SRR4302542.6303707/1‑94 (MQ=60)

          TAAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTA                                                                                      >  SRR4302542.1289319/1‑94 (MQ=60)

           AAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTAC                                                                                     >  SRR4302542.3277478/1‑94 (MQ=60)

            AATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACC                                                                                    >  SRR4302542.6038034/1‑94 (MQ=60)

             ATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCC                                                                                   <  SRR4302542.4147142/94‑1 (MQ=60)

              TATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCG                                                                                  <  SRR4302542.602572/94‑1 (MQ=60)

               ATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGA                                                                                 >  SRR4302542.4772640/1‑94 (MQ=60)

               ATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGA                                                                                 >  SRR4302542.861901/1‑94 (MQ=60)

               ATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGA                                                                                 >  SRR4302542.837867/1‑94 (MQ=60)

                TTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAA                                                                                >  SRR4302542.5313842/1‑94 (MQ=60)

                  TGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAAT                                                                              <  SRR4302542.1530813/94‑1 (MQ=60)

                    TGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGC                                                                            >  SRR4302542.4884310/1‑94 (MQ=60)

                     GATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCT                                                                           >  SRR4302542.4721730/1‑94 (MQ=60)

                     GATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCT                                                                           >  SRR4302542.7210956/1‑94 (MQ=60)

                     GATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCT                                                                           >  SRR4302542.2204152/1‑94 (MQ=60)

                      ATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTA                                                                          >  SRR4302542.4829036/1‑94 (MQ=60)

                      ATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTA                                                                          >  SRR4302542.7481651/1‑94 (MQ=60)

                      ATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTA                                                                          >  SRR4302542.4530283/1‑94 (MQ=60)

                        CTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAAT                                                                        >  SRR4302542.5911098/1‑94 (MQ=60)

                        CTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAAT                                                                        >  SRR4302542.5623915/1‑94 (MQ=60)

                        CTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAAT                                                                        <  SRR4302542.4940393/94‑1 (MQ=60)

                         TACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATT                                                                       >  SRR4302542.310372/1‑94 (MQ=60)

                           CAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTAC                                                                     >  SRR4302542.3395666/1‑94 (MQ=60)

                            AACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACA                                                                    >  SRR4302542.2213520/1‑94 (MQ=60)

                            AACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACA                                                                    >  SRR4302542.2078698/1‑94 (MQ=60)

                            AACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACA                                                                    >  SRR4302542.2672813/1‑94 (MQ=60)

                            AACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACA                                                                    <  SRR4302542.2988507/94‑1 (MQ=60)

                             ACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAG                                                                   >  SRR4302542.6118458/1‑94 (MQ=60)

                             ACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAG                                                                   >  SRR4302542.116898/1‑94 (MQ=60)

                              CACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGC                                                                  <  SRR4302542.5941029/94‑1 (MQ=60)

                              CACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGC                                                                  >  SRR4302542.1866065/1‑94 (MQ=60)

                                 GCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGT                                                               >  SRR4302542.4926005/1‑94 (MQ=60)

                                  CCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTT                                                              >  SRR4302542.804611/1‑94 (MQ=60)

                                   CTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTC                                                             >  SRR4302542.2747474/1‑94 (MQ=60)

                                   CTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTC                                                             >  SRR4302542.1489600/1‑94 (MQ=60)

                                    TTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCT                                                            >  SRR4302542.3050392/1‑94 (MQ=60)

                                    TTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCT                                                            >  SRR4302542.3439537/1‑94 (MQ=60)

                                    TTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCT                                                            >  SRR4302542.4314792/1‑94 (MQ=60)

                                      ATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTT                                                          >  SRR4302542.802911/1‑94 (MQ=60)

                                       TCTATTGCTTGTCCGCAAACGGACATGTCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTT                                                         >  SRR4302542.7292948/1‑94 (MQ=60)

                                         TATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGC                                                       >  SRR4302542.656200/1‑94 (MQ=60)

                                          ATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCT                                                      <  SRR4302542.923131/94‑1 (MQ=60)

                                          ATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCT                                                      >  SRR4302542.2202205/1‑94 (MQ=60)

                                            TGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTT                                                    <  SRR4302542.3596546/94‑1 (MQ=60)

                                            TGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTT                                                    >  SRR4302542.6679507/1‑94 (MQ=60)

                                            TGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTT                                                    >  SRR4302542.7171470/1‑94 (MQ=60)

                                             GCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTC                                                   >  SRR4302542.1528107/1‑94 (MQ=60)

                                              CTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCG                                                  >  SRR4302542.4397217/1‑94 (MQ=60)

                                              CTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCG                                                  >  SRR4302542.4766409/1‑94 (MQ=60)

                                              CTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCG                                                  >  SRR4302542.2607677/1‑94 (MQ=60)

                                              CTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCG                                                  <  SRR4302542.1528152/94‑1 (MQ=60)

                                                 GTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAA                                               >  SRR4302542.1520904/1‑94 (MQ=60)

                                                 GTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAA                                               >  SRR4302542.7392422/1‑94 (MQ=60)

                                                 GTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAA                                               >  SRR4302542.480791/1‑94 (MQ=60)

                                                   CCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACA                                             <  SRR4302542.677717/94‑1 (MQ=60)

                                                    CGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAA                                            >  SRR4302542.3595553/1‑94 (MQ=60)

                                                     GCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAAC                                           >  SRR4302542.5874565/1‑94 (MQ=60)

                                                      CAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACG                                          >  SRR4302542.5035278/1‑94 (MQ=60)

                                                      CAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACG                                          >  SRR4302542.2865012/1‑94 (MQ=60)

                                                        AACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTT                                        >  SRR4302542.3960888/1‑94 (MQ=60)

                                                        AACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTT                                        <  SRR4302542.4115220/94‑1 (MQ=60)

                                                          CGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTAT                                      <  SRR4302542.2279511/94‑1 (MQ=60)

                                                           GGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATC                                     >  SRR4302542.3221101/1‑94 (MQ=60)

                                                           GGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATC                                     <  SRR4302542.4608960/94‑1 (MQ=60)

                                                           GGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATC                                     >  SRR4302542.3280584/1‑94 (MQ=60)

                                                             ACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAA                                   <  SRR4302542.7459368/94‑1 (MQ=60)

                                                              CATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAca                                  >  SRR4302542.5084946/1‑92 (MQ=60)

                                                               ATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACA                                 >  SRR4302542.802806/1‑94 (MQ=60)

                                                               ATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACA                                 >  SRR4302542.418710/1‑94 (MQ=60)

                                                               ATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACA                                 >  SRR4302542.4967083/1‑94 (MQ=60)

                                                                TATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAG                                >  SRR4302542.1376046/1‑94 (MQ=60)

                                                                    AAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAA                            >  SRR4302542.1294766/1‑94 (MQ=60)

                                                                    AAGGGAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAA                            >  SRR4302542.7043263/1‑94 (MQ=60)

                                                                     AGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAG                           >  SRR4302542.5908272/1‑94 (MQ=60)

                                                                       GTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGCC                         <  SRR4302542.343652/94‑1 (MQ=60)

                                                                        TAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGCCG                        >  SRR4302542.7118042/1‑94 (MQ=60)

                                                                         AATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGCCGA                       >  SRR4302542.5394535/1‑94 (MQ=60)

                                                                          ATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGCCGAA                      >  SRR4302542.7525395/1‑94 (MQ=60)

                                                                          ATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGCCGAA                      >  SRR4302542.6492748/1‑94 (MQ=60)

                                                                          ATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGCCGAA                      >  SRR4302542.6636586/1‑94 (MQ=60)

                                                                            AAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGCCGAACT                    >  SRR4302542.4680228/1‑94 (MQ=60)

                                                                            AAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGCCGAACT                    <  SRR4302542.7538290/94‑1 (MQ=60)

                                                                            AAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGCCGAACT                    >  SRR4302542.5414949/1‑94 (MQ=60)

                                                                            AAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGCCGAACT                    >  SRR4302542.2797186/1‑94 (MQ=60)

                                                                            AAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGCCGAACT                    >  SRR4302542.272039/1‑94 (MQ=60)

                                                                               AAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGCCGAACTCTT                 <  SRR4302542.5193779/94‑1 (MQ=60)

                                                                                AAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGCCGAACTCTTC                >  SRR4302542.3940473/1‑94 (MQ=60)

                                                                                  GGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGCCGAACTCTTCAA              >  SRR4302542.5662256/1‑94 (MQ=60)

                                                                                   GTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGCCGAACTCTTCAAA             >  SRR4302542.4947492/1‑94 (MQ=60)

                                                                                   GTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGCCGAACTCTTCAAA             <  SRR4302542.3703737/94‑1 (MQ=60)

                                                                                     CGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGCCGAACTCTTCAAACA           >  SRR4302542.2483704/1‑94 (MQ=60)

                                                                                      GCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGCCGAACTCTTCAAACAG          >  SRR4302542.7325061/1‑94 (MQ=60)

                                                                                        CGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGCCGAACTCTTCAAACAGCA        <  SRR4302542.1056469/94‑1 (MQ=60)

                                                                                         GAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGCCGAACTCTTCAAACAGCAG       <  SRR4302542.5135430/94‑1 (MQ=60)

                                                                                         GAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGCCGAACTCTTCAAACAGCAG       >  SRR4302542.2441089/1‑94 (MQ=60)

                                                                                         GAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGCCGAACTCTTCAAACAGCAG       <  SRR4302542.127754/94‑1 (MQ=60)

                                                                                          AAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGCCGAACTCTTCAAACAGCAGC      >  SRR4302542.4876109/1‑94 (MQ=60)

                                                                                           AGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGCCGAACTCTTCAAACAGCAGCT     >  SRR4302542.4338247/1‑94 (MQ=60)

                                                                                             G‑ACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGCCGAACTCTTCAAACAGCAGCTCTG  >  SRR4302542.5975980/1‑94 (MQ=60)

                                                                                             G‑ACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGCCGAACTCTTCAAACAGCAGCTCTG  >  SRR4302542.4963026/1‑94 (MQ=60)

                                                                                             G‑ACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGCCGAACTCTTCAAACAGCAGCTCTG  >  SRR4302542.1361789/1‑94 (MQ=60)

TTATTGCATATAAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGC‑ACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGCCGAACTCTTCAAACAGCAGCTCTG  >  NC_000913/3077547‑3077733

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`