Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	1061	294.1	15065038	98.6%	14854127	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,078,245	C→G	G470G (GGG→GGC)	tktA ←	transketolase 1 isozyme

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,078,245	0	C	G	100.0%	945.2 / NA	284	G470G (GGG→GGC)	tktA	transketolase 1 isozyme
Reads supporting (aligned to +/- strand): ref base C (0/0); new base G (152/132); total (152/132)

Only 100 of 285 total aligned reads displayed.

CGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGCCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCATACGTACGGCGTTACGTGC  >  NC_000913/3078155‑3078334

 gcgGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCg                                                                                        >  2:5624627/1‑93 (MQ=255)

  cgGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGt                                                                                       >  2:446531/1‑93 (MQ=255)

   ggATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTc                                                                                      <  2:4387732/93‑1 (MQ=255)

   ggATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTc                                                                                      <  1:412705/93‑1 (MQ=255)

    gATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCt                                                                                     >  2:3516983/1‑93 (MQ=255)

    gATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCt                                                                                     <  2:3148323/93‑1 (MQ=255)

    gATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCt                                                                                     >  1:563333/1‑93 (MQ=255)

     aTTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCtt                                                                                    <  1:6587809/93‑1 (MQ=255)

     aTTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCtt                                                                                    >  2:537721/1‑93 (MQ=255)

      ttCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTc                                                                                   >  2:1168526/1‑93 (MQ=255)

      ttCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTc                                                                                   >  1:1950478/1‑93 (MQ=255)

      ttCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTc                                                                                   >  1:6217995/1‑93 (MQ=255)

          aCCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGccc                                                                               >  1:7006854/1‑93 (MQ=255)

             tGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGa                                                                            >  1:3442987/1‑93 (MQ=255)

             tGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGa                                                                            >  1:4272729/1‑93 (MQ=255)

               gTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGAcc                                                                          >  1:2933622/1‑93 (MQ=255)

               gTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGAcc                                                                          <  2:7435878/93‑1 (MQ=255)

                tCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCg                                                                         >  2:5790971/1‑93 (MQ=255)

                 cacaCGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGa                                                                        <  1:847/93‑1 (MQ=255)

                 cacaCGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGa                                                                        <  1:254255/93‑1 (MQ=255)

                 cacaCGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGa                                                                        <  1:5223214/93‑1 (MQ=255)

                 cacaCGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGa                                                                        <  2:3627658/93‑1 (MQ=255)

                  acacGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGAt                                                                       <  1:2624777/93‑1 (MQ=255)

                  acacGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGAt                                                                       >  2:4269403/1‑93 (MQ=255)

                   gacGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATg                                                                      <  1:5666117/92‑1 (MQ=255)

                   cacGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATg                                                                      >  1:5602687/1‑93 (MQ=255)

                   cacGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATg                                                                      <  1:4740486/93‑1 (MQ=255)

                    acGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATgg                                                                     >  2:5932460/1‑93 (MQ=255)

                     cGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGa                                                                    <  2:6112545/93‑1 (MQ=255)

                      ggACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAg                                                                   <  1:4539140/93‑1 (MQ=255)

                       gACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGt                                                                  <  2:735716/93‑1 (MQ=255)

                         cGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCg                                                                >  1:3360499/1‑93 (MQ=255)

                          gCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGt                                                               <  2:7122229/93‑1 (MQ=255)

                            cATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGGCGTCTTCGCCCAGACCGATGGAGTCGTgg                                                             >  1:3916779/1‑93 (MQ=255)

                            cATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTgg                                                             >  2:531441/1‑93 (MQ=255)

                             aTGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTggg                                                            >  2:4542995/1‑93 (MQ=255)

                             aTGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTggg                                                            >  2:6807748/1‑93 (MQ=255)

                             aTGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTggg                                                            >  2:2220975/1‑93 (MQ=255)

                             aTGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTggg                                                            <  2:4924824/93‑1 (MQ=255)

                             aTGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTggg                                                            >  1:2736761/1‑93 (MQ=255)

                              tGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGgt                                                           >  2:3949641/1‑93 (MQ=255)

                               gTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGgtg                                                          >  1:4906014/1‑93 (MQ=255)

                                tAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGgtgt                                                         >  1:1206224/1‑93 (MQ=255)

                                 aGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTa                                                        >  2:192674/1‑93 (MQ=255)

                                   aCATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTaaa                                                      >  2:2834092/1‑93 (MQ=255)

                                     aTGTTCGGGGTTACGCGCAGCGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAAcc                                                    >  1:6673181/1‑93 (MQ=255)

                                     aTGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAAcc                                                    >  1:954554/1‑93 (MQ=255)

                                      tGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCa                                                   >  2:2053880/1‑93 (MQ=255)

                                      tGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCa                                                   >  2:5134169/1‑93 (MQ=255)

                                         tCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCa                                                <  1:1000720/93‑1 (MQ=255)

                                         tCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCa                                                >  2:6634521/1‑93 (MQ=255)

                                         tCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCa                                                >  1:2097086/1‑93 (MQ=255)

                                          cGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCAc                                               <  1:2415252/93‑1 (MQ=255)

                                           ggggTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCAcc                                              >  1:3564847/1‑93 (MQ=255)

                                            gggTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCt                                             >  2:695424/1‑93 (MQ=255)

                                            gggTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCt                                             >  1:3809565/1‑93 (MQ=255)

                                             ggTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGGCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTg                                            >  2:5214851/1‑93 (MQ=255)

                                             ggTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTg                                            <  1:6421217/93‑1 (MQ=255)

                                              gTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGa                                           <  1:7385032/93‑1 (MQ=255)

                                               ttACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGAc                                          <  1:6762459/93‑1 (MQ=255)

                                                  cgcgCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCt                                       >  1:3075747/1‑93 (MQ=255)

                                                    cgcAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGt                                     <  1:2298354/93‑1 (MQ=255)

                                                    cgcAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGt                                     <  1:3994040/93‑1 (MQ=255)

                                                     gcAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGtt                                    >  1:6737474/1‑93 (MQ=255)

                                                     gcAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGtt                                    >  1:1697813/1‑93 (MQ=255)

                                                       agagAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTtc                                  <  2:2590619/93‑1 (MQ=255)

                                                        gagaAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTtca                                 >  2:1271175/1‑93 (MQ=255)

                                                          gaAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTtcatc                               >  2:3276811/1‑93 (MQ=255)

                                                          gaAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTtcatc                               <  1:4849294/93‑1 (MQ=255)

                                                          gaAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTtcatc                               >  2:20191/1‑93 (MQ=255)

                                                           aaGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTtcatca                              <  1:1877043/93‑1 (MQ=255)

                                                           aaGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTtcatca                              >  2:1496287/1‑93 (MQ=255)

                                                           aaGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCAGTTtcatca                              <  2:1351337/93‑1 (MQ=255)

                                                            aGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCAGTTTCATCAg                             <  2:4760460/93‑1 (MQ=255)

                                                               gACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAgcgc                          <  2:1744262/93‑1 (MQ=255)

                                                                aCCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGAGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCa                         <  1:668754/93‑1 (MQ=255)

                                                                 ccTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAg                        >  2:3361969/1‑93 (MQ=255)

                                                                  cTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGc                       <  2:625829/93‑1 (MQ=255)

                                                                  cTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGc                       <  2:1032044/93‑1 (MQ=255)

                                                                  cTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCAGTTTCATCAGCGCAGc                       <  2:6588997/93‑1 (MQ=255)

                                                                   tGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGcc                      >  1:108114/1‑93 (MQ=255)

                                                                    gCTCACCCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCa                     <  1:1449842/93‑1 (MQ=255)

                                                                    gCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCa                     <  2:799674/93‑1 (MQ=255)

                                                                      tCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCAta                   <  1:5258791/93‑1 (MQ=255)

                                                                       cAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCAtac                  >  2:6891437/1‑93 (MQ=255)

                                                                        aaCCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCAtann                 >  1:1777178/1‑91 (MQ=255)

                                                                        aaCCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCAtacg                 <  2:2703325/93‑1 (MQ=255)

                                                                          ccGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGGGTAAACCATCACCTGACGCTGTTTCATCAGCGCAACCAtacgta               >  1:5073686/1‑93 (MQ=255)

                                                                           cGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCAtacgtac              <  1:5027034/93‑1 (MQ=255)

                                                                           cGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCAtacgtac              <  2:3826667/93‑1 (MQ=255)

                                                                                ggTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCATACGTACGGCGt         >  2:6752557/1‑93 (MQ=255)

                                                                                  tGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCATACGTACGGCGTTa       >  1:3269830/1‑93 (MQ=255)

                                                                                  tGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCATACGTACGGCGTTa       >  1:2950407/1‑93 (MQ=255)

                                                                                  tGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCATACGTACGGCGTTa       <  2:5976084/93‑1 (MQ=255)

                                                                                    aGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCAGTTTCATCAGCGCAGCCATACGTACGGCGTTACg     <  2:2398595/93‑1 (MQ=255)

                                                                                     gTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCATACGTACGGCGTTACGt    <  1:2497793/93‑1 (MQ=255)

                                                                                      tCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCATACGTACGGCGTTACGTg   >  2:7060515/1‑93 (MQ=255)

                                                                                       cGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCATACGTACGGCGTTACGTGc  <  2:6523746/93‑1 (MQ=255)

                                                                                       cGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCATACGTACGGCGTTACGTGc  <  2:1646180/93‑1 (MQ=255)

                                                                                       cGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCAGTTTCATCAGCGCAGCCATACGTACGGCGTTACGTGc  <  2:618264/93‑1 (MQ=255)

CGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGCCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCATACGTACGGCGTTACGTGC  >  NC_000913/3078155‑3078334

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

GACCGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGCCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCATACGTACGGCGTTACGTGCGTAT  >  NC_000913/3078152‑3078338

GACCGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGG                                                                                               >  SRR4302542.1926899/1‑94 (MQ=60)

GACCGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGG                                                                                               <  SRR4302542.5170753/94‑1 (MQ=60)

GACCGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGG                                                                                               >  SRR4302542.1471502/1‑94 (MQ=60)

 ACCGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGC                                                                                              >  SRR4302542.3310861/1‑94 (MQ=60)

 ACCGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGC                                                                                              <  SRR4302542.4046050/94‑1 (MQ=60)

 ACCGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGC                                                                                              >  SRR4302542.6863098/1‑94 (MQ=60)

  CCGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCC                                                                                             <  SRR4302542.993745/94‑1 (MQ=60)

  CCGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCC                                                                                             <  SRR4302542.616220/94‑1 (MQ=60)

      GGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCT                                                                                         >  SRR4302542.6820621/1‑94 (MQ=60)

      GGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCT                                                                                         >  SRR4302542.568184/1‑94 (MQ=60)

      GGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCT                                                                                         <  SRR4302542.416258/94‑1 (MQ=60)

       GATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTT                                                                                        >  SRR4302542.6991506/1‑94 (MQ=60)

        ATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTC                                                                                       <  SRR4302542.6652716/94‑1 (MQ=60)

        ATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTC                                                                                       >  SRR4302542.6279239/1‑94 (MQ=60)

        ATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTC                                                                                       <  SRR4302542.4990396/94‑1 (MQ=60)

        ATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTC                                                                                       >  SRR4302542.1967347/1‑94 (MQ=60)

         TTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCG                                                                                      <  SRR4302542.157086/94‑1 (MQ=60)

         TTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCG                                                                                      >  SRR4302542.6344075/1‑94 (MQ=60)

         TTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCG                                                                                      <  SRR4302542.5089841/94‑1 (MQ=60)

           CAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCC                                                                                    >  SRR4302542.3690951/1‑94 (MQ=60)

            AACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCC                                                                                   >  SRR4302542.1434012/1‑94 (MQ=60)

            AACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCC                                                                                   >  SRR4302542.3982318/1‑94 (MQ=60)

            AACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCC                                                                                   >  SRR4302542.7075685/1‑94 (MQ=60)

             ACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCA                                                                                  >  SRR4302542.7463238/1‑94 (MQ=60)

             ACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCA                                                                                  <  SRR4302542.6250116/94‑1 (MQ=60)

             ACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCA                                                                                  <  SRR4302542.3488373/94‑1 (MQ=60)

             ACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCA                                                                                  >  SRR4302542.4082849/1‑94 (MQ=60)

              CCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAG                                                                                 >  SRR4302542.180891/1‑94 (MQ=60)

               CTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGA                                                                                >  SRR4302542.3475218/1‑94 (MQ=60)

               CTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGA                                                                                >  SRR4302542.3475217/1‑94 (MQ=60)

               CTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGA                                                                                >  SRR4302542.4314770/1‑94 (MQ=60)

                TGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGAC                                                                               >  SRR4302542.361119/1‑94 (MQ=60)

                 GGTCACACGGACGCCATGTAGACGTGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACC                                                                              >  SRR4302542.2026395/1‑94 (MQ=60)

                 GGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACC                                                                              >  SRR4302542.2961722/1‑94 (MQ=60)

                  GTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAAACCG                                                                             >  SRR4302542.6450605/1‑94 (MQ=60)

                    CACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGAT                                                                           <  SRR4302542.5274016/94‑1 (MQ=60)

                    CACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGAT                                                                           <  SRR4302542.852/94‑1 (MQ=60)

                    CACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGAT                                                                           <  SRR4302542.4032533/94‑1 (MQ=60)

                    CACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGAT                                                                           <  SRR4302542.256419/94‑1 (MQ=60)

                    CACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGAT                                                                           <  SRR4302542.961134/94‑1 (MQ=60)

                     ACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATG                                                                          <  SRR4302542.2650134/94‑1 (MQ=60)

                     ACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATG                                                                          >  SRR4302542.5657116/1‑94 (MQ=60)

                     ACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATG                                                                          >  SRR4302542.3388693/1‑94 (MQ=60)

                      GACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGG                                                                         <  SRR4302542.5721153/94‑1 (MQ=60)

                      CACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGG                                                                         <  SRR4302542.4786706/94‑1 (MQ=60)

                       ACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGA                                                                        <  SRR4302542.4864476/94‑1 (MQ=60)

                         GGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGT                                                                      >  SRR4302542.4465257/1‑94 (MQ=60)

                         GGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGT                                                                      <  SRR4302542.4583583/94‑1 (MQ=60)

                           ACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCG                                                                    >  SRR4302542.3392085/1‑94 (MQ=60)

                            CGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGT                                                                   <  SRR4302542.2555482/94‑1 (MQ=60)

                            CGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGT                                                                   >  SRR4302542.3894005/1‑94 (MQ=60)

                             GCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTG                                                                  <  SRR4302542.4749292/94‑1 (MQ=60)

                              CCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGGCGTCTTCGCCCAGACCGATGGAGTCGTGG                                                                 >  SRR4302542.3955428/1‑94 (MQ=60)

                              CCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGG                                                                 <  SRR4302542.5256351/94‑1 (MQ=60)

                              CCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGG                                                                 <  SRR4302542.2430912/94‑1 (MQ=60)

                               CATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGG                                                                >  SRR4302542.2763157/1‑94 (MQ=60)

                                ATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGT                                                               <  SRR4302542.2240547/94‑1 (MQ=60)

                                ATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGT                                                               >  SRR4302542.332681/1‑94 (MQ=60)

                                 TGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTG                                                              >  SRR4302542.4953735/1‑94 (MQ=60)

                                  GTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGT                                                             >  SRR4302542.1216596/1‑94 (MQ=60)

                                   TAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTA                                                            >  SRR4302542.2633503/1‑94 (MQ=60)

                                    AGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAA                                                           <  SRR4302542.6745191/94‑1 (MQ=60)

                                     GACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAA                                                          <  SRR4302542.201674/94‑1 (MQ=60)

                                      ACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAAC                                                         <  SRR4302542.6836848/94‑1 (MQ=60)

                                       CATGTTCGGGGTTACGCGCAGCGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACC                                                        >  SRR4302542.6738923/1‑94 (MQ=60)

                                       CATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGCCCGATGGGGTCGTGGGTGTCAACC                                                        >  SRR4302542.5621064/1‑94 (MQ=60)

                                       CATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACC                                                        >  SRR4302542.962749/1‑94 (MQ=60)

                                        ATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCA                                                       >  SRR4302542.4210335/1‑94 (MQ=60)

                                        ATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCA                                                       <  SRR4302542.2973701/94‑1 (MQ=60)

                                           TTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCA                                                    >  SRR4302542.2115197/1‑94 (MQ=60)

                                            TCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCAC                                                   <  SRR4302542.1009330/94‑1 (MQ=60)

                                            TCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGGAAACCATCAC                                                   >  SRR4302542.3171910/1‑94 (MQ=60)

                                             CGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACC                                                  >  SRR4302542.3598130/1‑94 (MQ=60)

                                             CGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACC                                                  <  SRR4302542.2438733/94‑1 (MQ=60)

                                              GGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCT                                                 <  SRR4302542.624442/94‑1 (MQ=60)

                                              GGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCT                                                 >  SRR4302542.3847261/1‑94 (MQ=60)

                                              GGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAA‑CATCACCTG                                                >  SRR4302542.3887831/1‑94 (MQ=60)

                                                GGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGA                                               <  SRR4302542.6484498/94‑1 (MQ=60)

                                                GGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGA                                               >  SRR4302542.1779733/1‑94 (MQ=60)

                                                 GTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGAC                                              <  SRR4302542.7457327/94‑1 (MQ=60)

                                                  TTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACG                                             <  SRR4302542.6828975/94‑1 (MQ=60)

                                                   TACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGC                                            <  SRR4302542.1576328/94‑1 (MQ=60)

                                                    ACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCT                                           >  SRR4302542.3105050/1‑94 (MQ=60)

                                                    ACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCT                                           >  SRR4302542.660961/1‑94 (MQ=60)

                                                      GCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGT                                         <  SRR4302542.7360380/94‑1 (MQ=60)

                                                      GCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGT                                         >  SRR4302542.4653758/1‑94 (MQ=60)

                                                       CGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTT                                        >  SRR4302542.1712522/1‑94 (MQ=60)

                                                       CGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTT                                        >  SRR4302542.6803795/1‑94 (MQ=60)

                                                       CGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTT                                        <  SRR4302542.1762146/94‑1 (MQ=60)

                                                       CGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTT                                        <  SRR4302542.2318100/94‑1 (MQ=60)

                                                       CGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTT                                        <  SRR4302542.4033440/94‑1 (MQ=60)

                                                        GCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTT                                       <  SRR4302542.4888433/94‑1 (MQ=60)

                                                        GCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTT                                       <  SRR4302542.6766384/94‑1 (MQ=60)

                                                          AGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCA                                     >  SRR4302542.2534324/1‑94 (MQ=60)

                                                           GAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCAGTTTCAT                                    <  SRR4302542.90772/94‑1 (MQ=60)

                                                            AGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATC                                   <  SRR4302542.6549542/94‑1 (MQ=60)

                                                             GAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCA                                  <  SRR4302542.4896514/94‑1 (MQ=60)

                                                             GAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCA                                  <  SRR4302542.2507876/94‑1 (MQ=60)

                                                             GAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCA                                  <  SRR4302542.6686756/94‑1 (MQ=60)

                                                             GAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCA                                  <  SRR4302542.4896515/94‑1 (MQ=60)

                                                              AAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAG                                 <  SRR4302542.4172778/94‑1 (MQ=60)

                                                              AAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAG                                 <  SRR4302542.1893286/94‑1 (MQ=60)

                                                               AGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGC                                >  SRR4302542.3013877/1‑94 (MQ=60)

                                                                GCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCG                               <  SRR4302542.1925545/94‑1 (MQ=60)

                                                                 CGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGC                              >  SRR4302542.2574154/1‑94 (MQ=60)

                                                                  GACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCA                             <  SRR4302542.2670123/94‑1 (MQ=60)

                                                                   ACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAG                            <  SRR4302542.2972225/94‑1 (MQ=60)

                                                                   ACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGAGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAG                            <  SRR4302542.674529/94‑1 (MQ=60)

                                                                    CCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCCGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGC                           >  SRR4302542.2944326/1‑94 (MQ=60)

                                                                    CCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGC                           <  SRR4302542.4956594/94‑1 (MQ=60)

                                                                     CTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCC                          >  SRR4302542.1189633/1‑94 (MQ=60)

                                                                     CTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCC                          >  SRR4302542.1157405/1‑94 (MQ=60)

                                                                     CTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCC                          >  SRR4302542.108998/1‑94 (MQ=60)

                                                                     CTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCC                          >  SRR4302542.6819261/1‑94 (MQ=60)

                                                                      TGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCA                         >  SRR4302542.2349543/1‑94 (MQ=60)

                                                                       GCTCACCCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCAT                        <  SRR4302542.1462351/94‑1 (MQ=60)

                                                                        CTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCAGTTTCATCAGCGCAGCCATA                       >  SRR4302542.5210748/1‑94 (MQ=60)

                                                                         TCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCATAC                      <  SRR4302542.3089779/94‑1 (MQ=60)

                                                                         TCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCATAC                      <  SRR4302542.4109596/94‑1 (MQ=60)

                                                                         TCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCATAC                      <  SRR4302542.5309896/94‑1 (MQ=60)

                                                                          CAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCATANN                     >  SRR4302542.1792577/1‑94 (MQ=60)

                                                                          CAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCATACG                     >  SRR4302542.2892780/1‑94 (MQ=60)

                                                                          CAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCATACG                     >  SRR4302542.5093916/1‑94 (MQ=60)

                                                                          CAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCAGTTTCATCAGCGCAGCCATACG                     >  SRR4302542.968677/1‑94 (MQ=60)

                                                                          CAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCAGTTTCATCAGCGCAGCCATACG                     <  SRR4302542.3142987/94‑1 (MQ=60)

                                                                          CAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGGGTAAACCATCACCTGACGCTGTTTTATCAGCGCAGCCATACG                     >  SRR4302542.6751607/1‑94 (MQ=60)

                                                                            ACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGGGTAAACCATCACCTGACGCTGTTTCATCAGCGCAACCATACGTA                   >  SRR4302542.5122995/1‑94 (MQ=60)

                                                                              CGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCCCCTGACGCTGTTTCATCAGCGCAGCCATACGTACG                 <  SRR4302542.181879/94‑1 (MQ=60)

                                                                              CGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCATACGTACG                 <  SRR4302542.5075836/94‑1 (MQ=60)

                                                                               GGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCATACGTACGG                <  SRR4302542.2475308/94‑1 (MQ=60)

                                                                               GGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCATACGTACGG                >  SRR4302542.5719076/1‑94 (MQ=60)

                                                                               GGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCAGTTTCATCAGCGCAGCCATACGTACGG                <  SRR4302542.7243964/94‑1 (MQ=60)

                                                                                GCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCATACGTACGGC               <  SRR4302542.568258/94‑1 (MQ=60)

                                                                                 CTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCATACGTACGGCG              <  SRR4302542.1054122/94‑1 (MQ=60)

                                                                                 CTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCATACGTACGGCG              >  SRR4302542.5674105/1‑94 (MQ=60)

                                                                                 CTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCATACGTACGGCG              >  SRR4302542.5687953/1‑94 (MQ=60)

                                                                                   GGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCATACGTACGGCGTT            <  SRR4302542.1292338/94‑1 (MQ=60)

                                                                                    GTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCATACGTACGGCGTTA           >  SRR4302542.3300692/1‑94 (MQ=60)

                                                                                    GTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCATACGTACGGCGTTA           >  SRR4302542.2978649/1‑94 (MQ=60)

                                                                                      GAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCATACGTACGGCGTTACG         <  SRR4302542.3437602/94‑1 (MQ=60)

                                                                                      GAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCATACGTACGGCGTTACG         <  SRR4302542.5322404/94‑1 (MQ=60)

                                                                                       AGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCATACGTACGGCGTTACGT        >  SRR4302542.4915103/1‑94 (MQ=60)

                                                                                        GTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCATACGTACGGCGTTACGTG       <  SRR4302542.2248482/94‑1 (MQ=60)

                                                                                        GTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCATACGTACGGCGTTACGTG       >  SRR4302542.5301621/1‑94 (MQ=60)

                                                                                        GTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCATACGTACGGCGTTACGTG       <  SRR4302542.2522017/94‑1 (MQ=60)

                                                                                         TCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCATACGTACGGCGTTACGTGC      <  SRR4302542.6776048/94‑1 (MQ=60)

                                                                                           GGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCATACGTACGGCGTTACGTGCGT    >  SRR4302542.63490/1‑94 (MQ=60)

                                                                                             GCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCATACGTACGGCGTTACGTGCGTAT  <  SRR4302542.35510/94‑1 (MQ=60)

GACCGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGCCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCATACGTACGGCGTTACGTGCGTAT  >  NC_000913/3078152‑3078338

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`