Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1061 294.1 15065038 98.6% 14854127 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,114,866 T→C N83S (AAC→AGC)  b2973 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,114,8660TC75.2% 478.2 / 204.7 295N83S (AAC→AGC) b2973hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (40/33);  new base C (105/117);  total (145/150)
Fisher's exact test for biased strand distribution p-value = 2.83e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01

Only 100 of 297 total aligned reads displayed.

GGATAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGTGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATC  >  NC_000913/3114776‑3114958
                                                                                          |                                                                                            
ggATAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGCTc                                                                                            <  1:2619258/93‑1 (MQ=255)
 gATAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCa                                                                                           >  2:1478657/1‑93 (MQ=255)
 gATAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGCTCa                                                                                           <  2:1012862/93‑1 (MQ=255)
 gATAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGCTCa                                                                                           <  2:1288499/93‑1 (MQ=255)
 gATAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACCTGCTCa                                                                                           <  2:6988028/93‑1 (MQ=255)
  aTAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAg                                                                                          >  1:661947/1‑93 (MQ=255)
     aCGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGCTCAGTGc                                                                                       >  1:5993186/1‑93 (MQ=255)
      cGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGCTCAGTGcc                                                                                      <  1:7349302/93‑1 (MQ=255)
      cGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGCTCAGTGcc                                                                                      <  1:6557754/93‑1 (MQ=255)
       gTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGTGCCa                                                                                     <  2:2225368/93‑1 (MQ=255)
       gTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGCTCAGTGCCa                                                                                     <  2:1366662/93‑1 (MQ=255)
        ttCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGTGCCAt                                                                                    <  2:2744717/93‑1 (MQ=255)
        ttCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGCTCAGTGCCAt                                                                                    >  2:2502698/1‑93 (MQ=255)
        ttCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGCTCAGTGCCAt                                                                                    <  2:5593962/93‑1 (MQ=255)
            tAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGTGCCATCGAc                                                                                >  1:4335032/1‑93 (MQ=255)
            tAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGCTCAGTGCCATCgnn                                                                                >  1:2185880/1‑91 (MQ=255)
            tAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGCTCAGTGCCATCGAc                                                                                <  1:2765108/93‑1 (MQ=255)
            tAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGCTCAGTGCCATCGAc                                                                                >  1:3040345/1‑93 (MQ=255)
            tAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGCTCAGTGCCATCGAc                                                                                >  1:616966/1‑93 (MQ=255)
             aGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGCTCAGTGCCATCGACa                                                                               <  1:1848193/93‑1 (MQ=255)
             aGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGCTCAGTGCCATCGACa                                                                               >  1:7275920/1‑93 (MQ=255)
             aGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGCTCAGTGCCATCGACa                                                                               >  1:1216000/1‑93 (MQ=255)
              gACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGCTCAGTGCCATCGACAg                                                                              >  2:951690/1‑93 (MQ=255)
                cccAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGCTCAGTGCCATCGACAGAc                                                                            >  1:344524/1‑93 (MQ=255)
                 ccAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGCTCAGTGCCATCGACAGAcc                                                                           <  1:135725/93‑1 (MQ=255)
                 ccAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGCTCAGTGCCATCGACAGAcc                                                                           <  1:5278350/93‑1 (MQ=255)
                 ccAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGCTCAGTGCCATCGACAGAcc                                                                           <  1:7169632/93‑1 (MQ=255)
                  cAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGCTCAGTGCCATCGACAGACCt                                                                          <  2:3654677/93‑1 (MQ=255)
                  cAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGCTCAGTGCCATCGACAGACCt                                                                          <  1:5837661/93‑1 (MQ=255)
                   aaaTGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGTGCCATCGACAGACCTg                                                                         >  1:1176564/1‑93 (MQ=255)
                    aaTGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGTGCCATCGACAGACCTGc                                                                        >  2:2993237/1‑93 (MQ=255)
                     aTGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGCTCAGTGCCATCGACAGACCTGCg                                                                       <  2:6664895/93‑1 (MQ=255)
                     aTGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGCTCAGTGCCATCGACAGACCTGCg                                                                       >  2:4658620/1‑93 (MQ=255)
                      tGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGTGCCATCGACAGACCTGCgg                                                                      >  2:6165722/1‑93 (MQ=255)
                      tGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGCTCAGTGCCATCGACAGACCTGCgg                                                                      >  2:2254636/1‑93 (MQ=255)
                      tGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGCTCAGTGCAATCGCCAAACCTCCgc                                                                      >  2:4960677/1‑92 (MQ=255)
                         ccGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGTGCCATCGACAGACCTGCGGCAt                                                                   >  1:4959785/1‑93 (MQ=255)
                         ccGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGCTCAGTGCCATCGACAGACCTGCGGCAt                                                                   >  2:3961557/1‑93 (MQ=255)
                         ccGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGCTCAGTGCCATCGACAGACCTGCGGCAt                                                                   >  1:943120/1‑93 (MQ=255)
                           gTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGTGCCATCGACAGACCTGCGGCATcc                                                                 >  2:4233056/1‑93 (MQ=255)
                           gTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGTGCCATCGACAGACCTGCGGCATcc                                                                 >  2:3159366/1‑93 (MQ=255)
                               cgcgcTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGCTCAGTGCCATCGACAGACCTGCGGCATCCAACa                                                             <  1:2481426/93‑1 (MQ=255)
                                gcgcTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGCTCAGTGCCATCGACAGACCTGCGGCATCCAACAg                                                            <  2:387839/93‑1 (MQ=255)
                                 cgcTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGTGCCATCGACAGACCTGCGGCATCCAACAGa                                                           <  2:4096516/93‑1 (MQ=255)
                                     gctgACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGCTCAGTGCCATCGACAGACCTGCGGCATCCAACAGACGca                                                       <  1:3642439/93‑1 (MQ=255)
                                      ctgACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGTGCCATCGACAGACCTGCGGCATCCAACAGACGcac                                                      <  1:4043769/93‑1 (MQ=255)
                                       tgACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGCTCAGTGCCATCGACAGACCTGCGGCATCCAACAGACGcaca                                                     >  1:2939828/1‑93 (MQ=255)
                                        gACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGCTCAGTGCCATCGACAGACCTGCGGCATCCAACAGACGCACaa                                                    >  2:1818927/1‑93 (MQ=255)
                                          cGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGCTCAGTGCCATCGACAGACCTGCGGCATCCAACAGACGCACaaaa                                                  >  1:3811859/1‑93 (MQ=255)
                                           gAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGCTCAGTGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAAc                                                 <  1:1896334/93‑1 (MQ=255)
                                           gAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGCTCAGTGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAAc                                                 <  1:2304767/93‑1 (MQ=255)
                                           gAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGCTCAGTCCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAAc                                                 <  1:7511620/93‑1 (MQ=255)
                                            aaCGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGCTCAGTGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAAcc                                                >  2:6942947/1‑93 (MQ=255)
                                            aaCGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGCTCAGTGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAAcc                                                >  2:6552808/1‑93 (MQ=255)
                                             aCGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGTGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCa                                               >  2:6784110/1‑93 (MQ=255)
                                             aCGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGCTCAGTGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCa                                               <  2:7500416/93‑1 (MQ=255)
                                               gcgGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGTGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGa                                             <  2:1736286/93‑1 (MQ=255)
                                               gcgGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGCTCAGTGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGa                                             <  1:6370006/93‑1 (MQ=255)
                                               gcgGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGCTCAGTGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGa                                             <  2:4749598/93‑1 (MQ=255)
                                               gcgGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGCTCAGTGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGa                                             <  2:5925931/93‑1 (MQ=255)
                                               gcgGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGCTCAGTGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGa                                             >  1:423025/1‑93 (MQ=255)
                                                 ggTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGTGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGAcg                                           <  1:5726190/93‑1 (MQ=255)
                                                 ggTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGCTCAGTGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGAcg                                           >  2:1878522/1‑93 (MQ=255)
                                                  gTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGCTCAGTGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGAcgc                                          >  1:6416899/1‑93 (MQ=255)
                                                  gTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGCTCAGTGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGAcgc                                          <  1:7232629/93‑1 (MQ=255)
                                                   ttCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGCTCAGTGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGAcgcg                                         >  2:2838615/1‑93 (MQ=255)
                                                    tCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGCTCAGTGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACgcgc                                        <  1:1670095/93‑1 (MQ=255)
                                                     cGGATACCCTTGCGGATCGTTATTTACTACCGACTTGCTCAGTGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGct                                       <  2:2603770/93‑1 (MQ=255)
                                                      ggATACCCTTGCGGATCGTTATTTACTACCGACTTGCTCAGTGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGctc                                      >  1:5004087/1‑93 (MQ=255)
                                                       gATACCCTTGCGGATCGTTATTTACTACCGACTTGCTCAGTGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGctct                                     <  2:4514215/93‑1 (MQ=255)
                                                        aTACCCTTGCGGATCGTTATTTACTACCGACTTGCTCAGTGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGctctc                                    <  1:5147695/93‑1 (MQ=255)
                                                        aTACCCTTGCGGATCGTTATTTACTACCGACTTGCTCAGTGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGctctc                                    <  1:5445593/93‑1 (MQ=255)
                                                         tACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGTGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGctctct                                   >  2:6395227/1‑93 (MQ=255)
                                                          aCCCTTGCGGATCGTTATTTACTACCGACTTGCTCAGTGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCtt                                  <  1:2948415/93‑1 (MQ=255)
                                                           cccTTGCGGATCGTTATTTACTACCGACTTGCTCAGTGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTc                                 <  1:5206846/93‑1 (MQ=255)
                                                             cTTGCGGATCGTTATTTACTACCGACTTGTTCAGTGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCt                               >  1:4571585/1‑93 (MQ=255)
                                                             cTTGCGGATCGTTATTTACTACCGACTTGCTCAGTGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCt                               <  2:3594552/93‑1 (MQ=255)
                                                              ttGCGGATCGTTATTTACTACCGACTTGTTCAGTGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCtt                              <  1:7124440/93‑1 (MQ=255)
                                                                gCGGATCGTTATTTACTACCGACTTGTTCAGTGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTaa                            <  1:871872/93‑1 (MQ=255)
                                                                gCGGATCGTTATTTACTACCGACTTGTTCAGTGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTaa                            <  2:6297645/93‑1 (MQ=255)
                                                                gCGGATCGTTATTTACTACCGACTTGCTCAGTGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTaa                            <  1:3520997/93‑1 (MQ=255)
                                                                gCGGATCGTTATTTACTACCGACTTGCTCAGTGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTaa                            <  1:6912330/93‑1 (MQ=255)
                                                                  ggATCGTTATTTACTACCGACTTGCTCAGTGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGa                          <  2:5227678/93‑1 (MQ=255)
                                                                   gATCGTTATTTACTACCGACTTGCTCAGTGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGAt                         <  1:5499720/93‑1 (MQ=255)
                                                                   gATCGTTATTTACTACCGACTTGCTCAGTGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGAt                         >  2:5808910/1‑93 (MQ=255)
                                                                   gATCGTTATTTACTACCGACTTGCTCAGTGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGAt                         <  2:6626925/93‑1 (MQ=255)
                                                                    aTCGTTATTTACTACCGACTTGTTCAGTGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGAtt                        >  2:332703/1‑93 (MQ=255)
                                                                    aTCGTTATTTACTACCGACTTGCTCAGTGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGAtt                        <  2:5660489/93‑1 (MQ=255)
                                                                        ttATTTACTACCGACTTGCTCAGTGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCtc                    >  2:5472611/1‑93 (MQ=255)
                                                                        ttATTTACTACCGACTTGCTCAGTGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCtc                    <  2:1409679/93‑1 (MQ=255)
                                                                           tttACTACCGACTTGCTCAGTGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCtcatc                 <  2:3807734/93‑1 (MQ=255)
                                                                            ttACTACCGACTTGTTCAGTGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCtcatca                <  1:5598909/93‑1 (MQ=255)
                                                                             tactacCGACTTGCTCAGTGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCAc               <  1:3307278/93‑1 (MQ=255)
                                                                               ctacCGACTTGCTCAGTGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGt             >  1:6798225/1‑93 (MQ=255)
                                                                                tacCGACTTGTTCAGTGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGtt            >  2:1990367/1‑93 (MQ=255)
                                                                                tacCGACTTGCTCAGTGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGtt            >  1:488589/1‑93 (MQ=255)
                                                                                 acCGACTTGCTCAGTGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGttt           <  2:2672793/93‑1 (MQ=255)
                                                                                     aCTTGCTCAGTGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCa       <  2:7314129/93‑1 (MQ=255)
                                                                                      cTTGCTCAGTGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCAt      <  2:82028/93‑1 (MQ=255)
                                                                                          ttCAGTGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATc  >  1:6954222/1‑93 (MQ=255)
                                                                                          |                                                                                            
GGATAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGTGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATC  >  NC_000913/3114776‑3114958

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GCAGGATAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGTGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCA  >  NC_000913/3114773‑3114959
                                                                                             |                                                                                             
GCAGGATAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGT                                                                                               <  SRR4302542.6889199/94‑1 (MQ=60)
 CAGGATAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGCT                                                                                              >  SRR4302542.2390461/1‑94 (MQ=60)
 CAGGATAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGCT                                                                                              <  SRR4302542.6877675/94‑1 (MQ=60)
  AGGATAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTT                                                                                             >  SRR4302542.2448840/1‑94 (MQ=60)
  AGGATAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGCTC                                                                                             >  SRR4302542.6559208/1‑94 (MQ=60)
   GGATAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGCTCA                                                                                            <  SRR4302542.2644568/94‑1 (MQ=60)
   GGATAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGCTCA                                                                                            >  SRR4302542.7385768/1‑94 (MQ=60)
   GGATAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGCTCA                                                                                            >  SRR4302542.647177/1‑94 (MQ=60)
    GATAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAG                                                                                           >  SRR4302542.667650/1‑94 (MQ=60)
     ATAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGCTCAGT                                                                                          >  SRR4302542.5345718/1‑94 (MQ=60)
       AACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGTGC                                                                                        >  SRR4302542.746708/1‑94 (MQ=60)
       AACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGCTCAGTGC                                                                                        >  SRR4302542.6051237/1‑94 (MQ=60)
       AACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGCTCAGTGC                                                                                        >  SRR4302542.260297/1‑94 (MQ=60)
       AACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGCTCAGTGC                                                                                        >  SRR4302542.949681/1‑94 (MQ=60)
         CGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGCTCAGTGCCA                                                                                      <  SRR4302542.7421277/94‑1 (MQ=60)
         CGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGCTCAGTGCCA                                                                                      <  SRR4302542.6622393/94‑1 (MQ=60)
         CGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGCTCAGTGCCA                                                                                      >  SRR4302542.2969111/1‑94 (MQ=60)
          GTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGCTCAGTGCCAT                                                                                     >  SRR4302542.2223452/1‑94 (MQ=60)
           TTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGTGCCATC                                                                                    >  SRR4302542.1613892/1‑94 (MQ=60)
           TTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGCTCAGTGCCATC                                                                                    <  SRR4302542.5768475/94‑1 (MQ=60)
            TCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGCTCAGTGCCATCG                                                                                   <  SRR4302542.6421930/94‑1 (MQ=60)
            TCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGCTCAGTGCCATCG                                                                                   <  SRR4302542.1303385/94‑1 (MQ=60)
              ATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGTGCCATCGAC                                                                                 >  SRR4302542.4377637/1‑94 (MQ=60)
              ATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGCTCAGTGCCATCGNN                                                                                 >  SRR4302542.2204708/1‑94 (MQ=60)
              ATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGCTCAGTGCCATCGAC                                                                                 >  SRR4302542.3069381/1‑94 (MQ=60)
              ATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGCTCAGTGCCATCGAC                                                                                 >  SRR4302542.622269/1‑94 (MQ=60)
              ATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGCTCAGTGCCATCGAC                                                                                 >  SRR4302542.4375242/1‑94 (MQ=60)
              ATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGCTCAGTGCCATCGAC                                                                                 <  SRR4302542.7201750/94‑1 (MQ=60)
               TAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGCTCAGTGCCATCGACA                                                                                >  SRR4302542.7347232/1‑94 (MQ=60)
               TAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGCTCAGTGCCATCGACA                                                                                >  SRR4302542.1226466/1‑94 (MQ=60)
               TAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGCTCAGTGCCATCGACA                                                                                <  SRR4302542.2791754/94‑1 (MQ=60)
                AGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGTGCCATCGACAG                                                                               <  SRR4302542.6135803/94‑1 (MQ=60)
                AGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGCTCAGTGCCATCGACAG                                                                               >  SRR4302542.324644/1‑94 (MQ=60)
                AGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGCTCAGTGCCATCGACAG                                                                               <  SRR4302542.1864184/94‑1 (MQ=60)
                 GACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGTGCCATCGACAGA                                                                              >  SRR4302542.7208702/1‑94 (MQ=60)
                  ACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGCTCAGTGCCATCGACAGAC                                                                             >  SRR4302542.347508/1‑94 (MQ=60)
                    CCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGTGCCATCGACAGACCT                                                                           <  SRR4302542.6176628/94‑1 (MQ=60)
                    CCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGTGCCATCGACAGACCT                                                                           >  SRR4302542.1868687/1‑94 (MQ=60)
                    CCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGCTCAGTGCCATCGACAGACCT                                                                           <  SRR4302542.7179297/94‑1 (MQ=60)
                    CCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGCTCAGTGCCATCGACAGACCT                                                                           <  SRR4302542.5329676/94‑1 (MQ=60)
                    CCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGCTCAGTGCCATCGACAGACCT                                                                           <  SRR4302542.5028879/94‑1 (MQ=60)
                    CCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGCTCAGTGCCATCGACAGACCT                                                                           <  SRR4302542.136862/94‑1 (MQ=60)
                    CCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGCTCAGTGCCATCGACAGACCT                                                                           <  SRR4302542.7239955/94‑1 (MQ=60)
                     CAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGTGCCATCGACAGACCTG                                                                          <  SRR4302542.5586989/94‑1 (MQ=60)
                     CAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGTGCCATCGACAGACCTG                                                                          >  SRR4302542.1186732/1‑94 (MQ=60)
                     CAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGCTCAGTGCCATCGACAGACCTG                                                                          >  SRR4302542.5422729/1‑94 (MQ=60)
                     CAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGCTCAGTGCCATCGACAGACCTG                                                                          <  SRR4302542.5894293/94‑1 (MQ=60)
                      AAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGCTCAGTGCCATCGACAGACCTGC                                                                         <  SRR4302542.5268974/94‑1 (MQ=60)
                       AATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGCTCAGTGCCATCGACAGACCTGCG                                                                        <  SRR4302542.5589192/94‑1 (MQ=60)
                        ATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGCTCAGTGCCATCGACAGACCTGCGG                                                                       <  SRR4302542.7274727/94‑1 (MQ=60)
                        ATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGCTCAGTGCCATCGACAGACCTGCGG                                                                       >  SRR4302542.6767933/1‑94 (MQ=60)
                           CCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGTGCCATCGACAGACCTGCGGCAT                                                                    <  SRR4302542.3661054/94‑1 (MQ=60)
                           CCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGTGCCATCGACAGACCTGCGGCAT                                                                    >  SRR4302542.5007966/1‑94 (MQ=60)
                           CCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGCTCAGTGCCATCGACAGACCTGCGGCAT                                                                    >  SRR4302542.951237/1‑94 (MQ=60)
                           CCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGCTCAGTGCCATCGACAGACCTGCGGCAT                                                                    >  SRR4302542.5328815/1‑94 (MQ=60)
                           CCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGCTCAGTGCCATCGACAGACCTGCGGCAT                                                                    <  SRR4302542.6979304/94‑1 (MQ=60)
                            CCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGCTCAGTGCCATCGACAGACCTGCGGCATC                                                                   <  SRR4302542.5082535/94‑1 (MQ=60)
                             CGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGCTCAGTGCCATCGACAGACCTGCGGCATCC                                                                  <  SRR4302542.6668985/94‑1 (MQ=60)
                             CGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGCTCAGTGCCATCGACAGACCTGCGGCATCC                                                                  <  SRR4302542.6534732/94‑1 (MQ=60)
                              GTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGCTCAGTGCCATCGACAGACCTGCGGCATCCA                                                                 <  SRR4302542.6472533/94‑1 (MQ=60)
                               TTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGCTCAGTGCCATCGACAGACCTGCGGCATCCAA                                                                >  SRR4302542.2976267/1‑94 (MQ=60)
                                  CGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGCTCAGTGCCATCGACAGACCTGCGGCATCCAACAG                                                             <  SRR4302542.2505483/94‑1 (MQ=60)
                                    CGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTGCCGACTTGCTCAGTGCCATCGACAGACCTGCGGCATCCAACAGAC                                                           <  SRR4302542.4247973/94‑1 (MQ=60)
                                      CTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGTGCCATCGACAGACCTGCGGCATCCAACAGACGC                                                         <  SRR4302542.3757713/94‑1 (MQ=60)
                                        GCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGCTCAGTGCCATCGACAGACCTGCGGCATCCAACAGACGCAC                                                       <  SRR4302542.3676423/94‑1 (MQ=60)
                                         CTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGTGCCATCGACAGACCTGCGGCATCCAACAGACGCACA                                                      <  SRR4302542.4083667/94‑1 (MQ=60)
                                         CTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGCTCAGTGCCATCGACAGACCTGCGGCATCCAACAGACGCACA                                                      <  SRR4302542.1842485/94‑1 (MQ=60)
                                         CTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGCTCAGTGCCATCGACAGACCTGCGGCATCCAACAGACGCACA                                                      >  SRR4302542.2967983/1‑94 (MQ=60)
                                            ACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGCTCAGTGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAA                                                   >  SRR4302542.3849570/1‑94 (MQ=60)
                                            ACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGCTCAGTGCCATCGACACACCTGCGGCATCCAACAGACGCACAAAA                                                   >  SRR4302542.6984444/1‑94 (MQ=60)
                                             CGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGTGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAAC                                                  >  SRR4302542.1867217/1‑94 (MQ=60)
                                             CGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGCTCAGTGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAAC                                                  <  SRR4302542.7288513/94‑1 (MQ=60)
                                              GAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGCTCAGTGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACC                                                 <  SRR4302542.1912743/94‑1 (MQ=60)
                                              GAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGCTCAGTGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACC                                                 >  SRR4302542.1225572/1‑94 (MQ=60)
                                              GAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGCTCAGTGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACC                                                 <  SRR4302542.2324563/94‑1 (MQ=60)
                                              GAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGCTCAGTGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACC                                                 <  SRR4302542.5251906/94‑1 (MQ=60)
                                              GAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGCTCAGTCCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACC                                                 <  SRR4302542.7585494/94‑1 (MQ=60)
                                               AACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGCTCAGTGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCA                                                <  SRR4302542.5833658/94‑1 (MQ=60)
                                               AACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGCTCAGTGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCA                                                <  SRR4302542.5256419/94‑1 (MQ=60)
                                               AACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGCTCAGTGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCA                                                <  SRR4302542.7464576/94‑1 (MQ=60)
                                                 CGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGCTCAGTGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGA                                              >  SRR4302542.426656/1‑94 (MQ=60)
                                                  GCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGTGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGAC                                             <  SRR4302542.3552804/94‑1 (MQ=60)
                                                  GCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGCTCAGTGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGAC                                             <  SRR4302542.4961467/94‑1 (MQ=60)
                                                  GCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGCTCAGTGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGAC                                             <  SRR4302542.6432735/94‑1 (MQ=60)
                                                    GGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGTGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGC                                           <  SRR4302542.5781765/94‑1 (MQ=60)
                                                    GGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGCTCAGTGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGC                                           <  SRR4302542.731346/94‑1 (MQ=60)
                                                    GGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGCTCAGTGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGC                                           >  SRR4302542.6480137/1‑94 (MQ=60)
                                                     GTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGTGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCG                                          <  SRR4302542.7030826/94‑1 (MQ=60)
                                                     GTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGCTCAGTGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCG                                          <  SRR4302542.7303571/94‑1 (MQ=60)
                                                       TCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGCTCAGTGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCT                                        >  SRR4302542.1001025/1‑94 (MQ=60)
                                                       TCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGCTCAGTGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCT                                        <  SRR4302542.881269/94‑1 (MQ=60)
                                                       TCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGCTCAGTGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCT                                        <  SRR4302542.1684530/94‑1 (MQ=60)
                                                        CGGATACCCTTGCGGATCGTTATTTACTACCGACTTGCTCAGTGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTC                                       >  SRR4302542.5052690/1‑94 (MQ=60)
                                                        CGGATACCCTTGCGGATCGTTATTTACTACCGACTTGCTCAGTGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTC                                       >  SRR4302542.3933889/1‑94 (MQ=60)
                                                         GGATACCCTTGCGGATCGTTATTTACTACCGACTTGCTCAGTGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCT                                      >  SRR4302542.7325496/1‑94 (MQ=60)
                                                         GGATACCCTTGCGGATCGTTATTTACTACCGACTTGCTCAGTGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCT                                      >  SRR4302542.80903/1‑94 (MQ=60)
                                                         GGATACCCTTGCGGATCGTTATTTACTACCGACTTGCTCAGTGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCT                                      >  SRR4302542.465556/1‑94 (MQ=60)
                                                         GGATACCCTTGCGGATCGTTATTTACTACCGACTTGCTCAGTGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCT                                      >  SRR4302542.5001113/1‑94 (MQ=60)
                                                         GGATACCCTTGCGGATCGTTATTTACTACCGACTTGCTCAGTGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCT                                      >  SRR4302542.5159992/1‑94 (MQ=60)
                                                         GGATACCCTTGCGGATCGTTATTTACTACCGACTTGCTCAGTGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCT                                      >  SRR4302542.5028922/1‑94 (MQ=60)
                                                          GATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGTGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTC                                     <  SRR4302542.3894367/94‑1 (MQ=60)
                                                          GATACCCTTGCGGATCGTTATTTACTACCGACTTGCTCAGTGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTC                                     >  SRR4302542.824216/1‑94 (MQ=60)
                                                           ATACCCTTGCGGATCGTTATTTACTACCGACTTGCTCAGTGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCT                                    <  SRR4302542.5498493/94‑1 (MQ=60)
                                                           ATACCCTTGCGGATCGTTATTTACTACCGACTTGCTCAGTGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCT                                    <  SRR4302542.5197732/94‑1 (MQ=60)
                                                           ATACCCTTGCGGATCGTTATTTACTACCGACTTGCTCAGTGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCT                                    <  SRR4302542.2126864/94‑1 (MQ=60)
                                                           ATACCCTTGCGGATCGTTATTTACTACCGACTTGCTCAGTGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCT                                    >  SRR4302542.5503543/1‑94 (MQ=60)
                                                            TACCCTTGCGGATCGTTATTTACTACCGACTTGCTCAGTGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTT                                   <  SRR4302542.2789539/94‑1 (MQ=60)
                                                             ACCCTTGCGGATCGTTATTTACTACCGACTTGCTCAGTGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTC                                  <  SRR4302542.2976642/94‑1 (MQ=60)
                                                             ACCCTTGCGGATCGTTATTTACTACCGACTTGCTCAGTGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTC                                  <  SRR4302542.5891458/94‑1 (MQ=60)
                                                              CCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGTGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCC                                 >  SRR4302542.4294363/1‑94 (MQ=60)
                                                              CCCTTGCGGATCGTTATTTACTACCGACTTGCTCAGTGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCC                                 >  SRR4302542.4379132/1‑94 (MQ=60)
                                                              CCCTTGCGGATCGTTATTTACTACCGACTTGCTCAGTGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCC                                 <  SRR4302542.5257484/94‑1 (MQ=60)
                                                              CCCTTGCGGATCGTTATTTACTACCGACTTGCTCAGTGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCC                                 >  SRR4302542.3526110/1‑94 (MQ=60)
                                                               CCTTGCGGATCGTTATTTACTACCGACTTGTTCAGTGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCT                                >  SRR4302542.4616321/1‑94 (MQ=60)
                                                               CCTTGCGGATCGTTATTTACTACCGACTTGTTCAGTGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCT                                <  SRR4302542.2899937/94‑1 (MQ=60)
                                                               CCTTGCGGATCGTTATTTACTACCGACTTGCTCAGTGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCT                                >  SRR4302542.3050684/1‑94 (MQ=60)
                                                               CCTTGCGGATCGTTATTTACTACCGACTTGCTCAGTGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCT                                >  SRR4302542.1297297/1‑94 (MQ=60)
                                                                 TTGCGGATCGTTATTTACTACCGACTTGTTCAGTGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTA                              <  SRR4302542.7194365/94‑1 (MQ=60)
                                                                 TTGCGGATCGTTATTTACTACCGACTTGCTCAGTGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTA                              <  SRR4302542.4550568/94‑1 (MQ=60)
                                                                   GCGGATCGTTATTTACTACCGACTTGTTCAGTGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAG                            <  SRR4302542.879391/94‑1 (MQ=60)
                                                                   GCGGATCGTTATTTACTACCGACTTGCTCAGTGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAG                            <  SRR4302542.3553903/94‑1 (MQ=60)
                                                                   GCGGATCGTTATTTACTACCGACTTGCTCAGTGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAG                            <  SRR4302542.6980191/94‑1 (MQ=60)
                                                                   GCGGATCGTTATTTACTACCGACTTGCTCAGTGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAG                            <  SRR4302542.3258837/94‑1 (MQ=60)
                                                                     GGATCGTTATTTACTACCGACTTGCTCAGTGCCTTCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGAT                          >  SRR4302542.21942/1‑94 (MQ=60)
                                                                      GATCGTTATTTACTACCGACTTGCTCAGTGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATT                         >  SRR4302542.2574695/1‑94 (MQ=60)
                                                                      GATCGTTATTTACTACCGACTTGCTCAGTGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATT                         <  SRR4302542.5553141/94‑1 (MQ=60)
                                                                      GATCGTTATTTACTACCGACTTGCTCAGTGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATT                         >  SRR4302542.5531891/1‑94 (MQ=60)
                                                                      GATCGTTATTTACTACCGACTTGCTCAGTGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATT                         >  SRR4302542.591149/1‑94 (MQ=60)
                                                                      GACCGTTATTTACTACCGACTTGCTCAGTGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATT                         >  SRR4302542.674858/1‑94 (MQ=60)
                                                                       ATCGTTATTTACTACCGACTTGCTCAGTGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTG                        >  SRR4302542.2297128/1‑94 (MQ=60)
                                                                       ATCGTTATTTACTACCGACTTGCTCAGTGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTG                        <  SRR4302542.7000010/94‑1 (MQ=60)
                                                                       ATCGTTATTTACTACCGACTTGCTCAGTGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTG                        >  SRR4302542.280653/1‑94 (MQ=60)
                                                                         CGTTATTTACTACCGACTTGCTCAGTGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCT                      >  SRR4302542.4166315/1‑94 (MQ=60)
                                                                           TTATTTACTACCGACTTGCTCAGTGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCA                    >  SRR4302542.478651/1‑94 (MQ=60)
                                                                           TTATTTACTACCGACTTGCTCAGTGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTCAAGATTGCTCA                    >  SRR4302542.6255333/1‑94 (MQ=60)
                                                                            TATTTACTACCGACTTGTTCAGTGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCAT                   <  SRR4302542.6960689/94‑1 (MQ=60)
                                                                             ATTTACTACCGACTTGCTCAGTGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATC                  >  SRR4302542.1640024/1‑94 (MQ=60)
                                                                              TTTACTACCGACTTGCTCAGTGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCA                 >  SRR4302542.3018878/1‑94 (MQ=60)
                                                                              TTTACTACCGACTTGCTCAGTGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCA                 <  SRR4302542.3638287/94‑1 (MQ=60)
                                                                               TTACTACCGACTTGTTCAGTGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCAC                <  SRR4302542.5653303/94‑1 (MQ=60)
                                                                                TACTACCGACTTGTTCAGTGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACG               >  SRR4302542.3370524/1‑94 (MQ=60)
                                                                                TACTACCGACTTGCTCAGTGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACG               <  SRR4302542.3338462/94‑1 (MQ=60)
                                                                                 ACTACCGACTTGCTCAGTGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGT              >  SRR4302542.6865075/1‑94 (MQ=60)
                                                                                 ACTACCGACTTGCTCAGTGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGT              >  SRR4302542.3752572/1‑94 (MQ=60)
                                                                                  CTACCGACTTGCTCAGTGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTT             >  SRR4302542.6677317/1‑94 (MQ=60)
                                                                                  CTACCGACTTGCTCAGTGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTT             <  SRR4302542.3366126/94‑1 (MQ=60)
                                                                                  CTACCGACTTGCTCAGTGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTT             >  SRR4302542.492811/1‑94 (MQ=60)
                                                                                  CTACCGACTTGCTCAGTGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTT             >  SRR4302542.7369243/1‑94 (MQ=60)
                                                                                    ACCGACTTGCTCAGTGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTT           >  SRR4302542.1697917/1‑94 (MQ=60)
                                                                                     CCGACTTGCTCAGTGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCACTTCCTTAAGATTGCTCATCACGTTTTC          <  SRR4302542.5716822/94‑1 (MQ=60)
                                                                                      CGACTTGTTCAGTGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCC         >  SRR4302542.996201/1‑94 (MQ=60)
                                                                                       GACTTGCTCAGTGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCA        >  SRR4302542.2752797/1‑94 (MQ=60)
                                                                                         CTTGCTCAGTGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATG      >  SRR4302542.2450723/1‑94 (MQ=60)
                                                                                           TGCTCAGTGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGAT    <  SRR4302542.4525982/94‑1 (MQ=60)
                                                                                           TGCTCAGTGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGAT    >  SRR4302542.3524538/1‑94 (MQ=60)
                                                                                            GTTCAGTGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATC   >  SRR4302542.7022549/1‑94 (MQ=60)
                                                                                            GCTCAGTGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATC   <  SRR4302542.6414877/94‑1 (MQ=60)
                                                                                            GCTCAGTGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATC   <  SRR4302542.1366842/94‑1 (MQ=60)
                                                                                             CTCAGTGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCA  >  SRR4302542.2895523/1‑94 (MQ=60)
                                                                                             CTCAGTGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCA  >  SRR4302542.1027160/1‑94 (MQ=60)
                                                                                             CTCAGTGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCA  >  SRR4302542.1015597/1‑94 (MQ=60)
                                                                                             |                                                                                             
GCAGGATAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGTGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCA  >  NC_000913/3114773‑3114959

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑