Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	1061	294.1	15065038	98.6%	14854127	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,197,947	A→G	D220D (GAT→GAC)	ygiF ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,197,947	0	A	G	88.3%	675.2 / 69.4	265	D220D (GAT→GAC)	ygiF	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (15/16); new base G (113/121); total (128/137)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 273 total aligned reads displayed.

CGCCGCATCGTTGCCGCGTACCCACAGTTCTTCATGATACTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACATCGGCTTTTGCCGCAACATGCAAAATGGTGGTCGGTTTGATTTCACGCGCCGGATTGCCCTGCGCCAGATGATAGCCACGCGCCGCTTTGCT  >  NC_000913/3197857‑3198039

cgccgcATCGTTGCCGCGTACCCACAGTTCTTCATGATACTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACGTc                                                                                            >  2:1761698/1‑93 (MQ=255)

cgccgcATCGTTGCCGCGTACCCACAGTTCTTCATGATACTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACGTc                                                                                            <  1:1333994/93‑1 (MQ=255)

cgccgcATCGTTGCCGCGTACCCACAGTTCTTCATGATACTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACGTc                                                                                            <  1:5550475/93‑1 (MQ=255)

 gccgcATCGTTGCCGCGTACCCACAGTTCTTCATGATACTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACGTCg                                                                                           >  1:1637473/1‑93 (MQ=255)

  ccgcATCGTTGCCGCGTACCCACAGTTCTTCATGATACTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACGTCgg                                                                                          <  1:741574/93‑1 (MQ=255)

    gcATCGTTGCCGCGTACCCACAGTTCTTCATGATACTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACGTCGGCt                                                                                        >  2:3813101/1‑93 (MQ=255)

    gcATCGTTGCCGCGTACCCACAGTTCTTCATGATACTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACGTCGGCt                                                                                        >  2:1691350/1‑93 (MQ=255)

    gcATCGTTGCCGCGTACCCACAGTTCTTCATGATACTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACATCGGCt                                                                                        <  1:5885306/93‑1 (MQ=255)

     cATCGTTGCCGCGTACCCACAGTTCTTCATGATACTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACGTCGGCtt                                                                                       <  1:6648218/93‑1 (MQ=255)

     cATCGTTGCCGCGTACCCACAGTTCTTCATGATACTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACATCGGCtt                                                                                       >  2:6441761/1‑93 (MQ=255)

      aTCGTTGCCGCGTACCCACAGTTCTTCATGATACTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACGTCGGCttt                                                                                      <  1:5677504/93‑1 (MQ=255)

      aTCGTTGCCGCGTACCCACAGTTCTTCATGATACTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACGTCGGCttt                                                                                      >  1:5173383/1‑93 (MQ=255)

       tCGTTGCCGCGTACCCACAGTTCTTCATGATACTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACGTCGGCtttt                                                                                     >  1:1838558/1‑93 (MQ=255)

       tCGTTGCCGCGTACCCACAGTTCTTCATGATACTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACGTCGGCtttt                                                                                     >  2:5274417/1‑93 (MQ=255)

       tCGTTGCCGCGTACCCACAGTTCTTCATGATACTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACGTCGGCtttt                                                                                     <  2:4779419/93‑1 (MQ=255)

       tCGTTGCCGCGTACCCACAGTTCTTCATGATACTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACATCGGCtttt                                                                                     >  2:5250303/1‑93 (MQ=255)

         gTTGCCGCGTACCCACAGTTCTTCATGATACTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACGTCGGCTTTTGc                                                                                   >  1:3629046/1‑93 (MQ=255)

         gTTGCCGCGTACCCACAGTTCTTCATGATACTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACGTCGGCTTTTGc                                                                                   <  2:2107911/93‑1 (MQ=255)

         gTTGCCGCGTACCCACAGTTCTTCATGATACTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACGTCGGCTTTTGc                                                                                   <  2:2840256/93‑1 (MQ=255)

          ttGCCGCGTACCCACAGTTCTTCATGATACTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACGTCGGCTTTTGcc                                                                                  <  2:153613/93‑1 (MQ=255)

            gCCGCGTACCCACAGTTCTTCATGATACTGCCATTGCGCTAACGTCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACGTCGGCTTTTGCCGc                                                                                >  2:3930334/1‑93 (MQ=255)

              cgcgTACCCACAGTTCTTCATGATACTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACGTCGGCTTTTGCCGCaa                                                                              >  1:926286/1‑93 (MQ=255)

              cgcgTACCCACAGTTCTTCATGATACTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACGTCGGCTTTTGCCGCaa                                                                              >  1:1000848/1‑93 (MQ=255)

               gcgTACCCACAGTTCTTCATGATACTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACGTCGGCTTTTGCCGCAAc                                                                             <  1:6204163/93‑1 (MQ=255)

                cgTACCCACAGTTCTTCATGATACTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACGTCGGCTTTTGCCGCAACa                                                                            <  2:6748100/93‑1 (MQ=255)

                cgTACCCACAGTTCTTCATGATACTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACGTCGGCTTTTGCCGCAACa                                                                            <  2:5584111/93‑1 (MQ=255)

                  tACCCACAGTTCTTCATGATACTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACGTCGGCTTTTGCCGCAACATg                                                                          >  2:6608924/1‑93 (MQ=255)

                    cccACAGTTCTTCATGATACTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACATCGGCTTTTGCCGCAACATGCa                                                                        >  1:6673735/1‑93 (MQ=255)

                     ccACAGTTCTTCATGATACTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACGTCGGCTTTTGCCGCAACATGCaa                                                                       >  2:5781759/1‑93 (MQ=255)

                     ccACAGTTCTTCATGATACTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACGTCGGCTTTTGCCGCAACATGCaa                                                                       >  1:5383237/1‑93 (MQ=255)

                     ccACAGTTCTTCATGATACTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACGTCGGCTTTTGCCGCAACATGCaa                                                                       >  1:4242351/1‑93 (MQ=255)

                      cacaGTTCTTCATGATACTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACGTCGGCTTTTGCCGCAACATGCaaa                                                                      <  2:1557917/93‑1 (MQ=255)

                      cacaGTTCTTCATGATACTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACGTCGGCTTTTGCCGCAACATGCaaa                                                                      <  1:4008196/93‑1 (MQ=255)

                      cacaGTTCTTCATGATACTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACGTCGGCTTTTGCCGCAACATGCaaa                                                                      >  1:2299150/1‑93 (MQ=255)

                       acaGTTCTTCATGATACTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACGTCGGCTTTTGCCGCAACATGCaaaa                                                                     <  1:5098713/93‑1 (MQ=255)

                       acaGTTCTTCATGATACTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACGTCGGCTTTTGCCGCAACATGCaaaa                                                                     <  1:3171644/93‑1 (MQ=255)

                       acaGTTCTTCATGATACTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACATCGGCTTTTGCCGCAACATGCaaaa                                                                     >  2:4546558/1‑93 (MQ=255)

                         aGTTCTTCATGATACTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACGTCGGCTTTTGCCGCAACATGCAAAAtg                                                                   <  2:5676840/93‑1 (MQ=255)

                         aGTTCTTCATGATACTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACGTCGGCTTTTGCCGCAACATGCAAAAtg                                                                   >  1:6955237/1‑93 (MQ=255)

                          gTTCTTCATGATACTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACGTCGGCTTTTGCCGCAACATGCAAAAtgg                                                                  <  1:500504/93‑1 (MQ=255)

                           ttcttcATGATACTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACGTCGGCTTTTGCCGCAACATGCAAAAtggt                                                                 >  2:6488198/1‑93 (MQ=255)

                           ttcttcATGATACTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACGTCGGCTTTTGCCGCAACATGCAAAAtggt                                                                 <  1:7174804/93‑1 (MQ=255)

                               tcATGATACTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACGTCGGCTTTTGCCGCAACATGCAAAATGGTGGTc                                                             <  2:2899126/93‑1 (MQ=255)

                                cATGATACTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACGTCGGCTTTTGCCGCAACATGCAAAATGGTGGTCg                                                            <  2:3304420/93‑1 (MQ=255)

                                  tGATACTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACGTCGGCTTTTGCCGCAACATGCAAAATGGTGGTCGGt                                                          >  2:4420934/1‑93 (MQ=255)

                                  tGATACTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACGTCGGCTTTTGCCGCAACATGCAAAATGGTGGTCGGt                                                          >  2:7072508/1‑93 (MQ=255)

                                   gATACTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACGTCGGCTTTTGCCGCAACATGCAAAATGGTGGTCGGtt                                                         >  1:3806728/1‑93 (MQ=255)

                                   gATACTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACGTCGGCTTTTGCCGCAACATGCAAAATGGTGGTCGGtt                                                         >  2:741750/1‑93 (MQ=255)

                                   gATACTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACATCGGCTTTTGCCGCAACATGCAAAATGGTGGTCGGtt                                                         <  2:3419345/93‑1 (MQ=255)

                                    aTACTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACGTCGGCTTTTGCCGCAACATGCAAAATGGTGGTCGGttt                                                        <  1:3074242/93‑1 (MQ=255)

                                       cTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACGTCGGCTTTTGCCGCAACATGCAAAATGGTGGTCGGTTTGAt                                                     >  1:705301/1‑93 (MQ=255)

                                       cTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACGTCGGCTTTTGCCGCAACATGCAAAATGGTGGTCGGTTTGAt                                                     >  2:4151460/1‑93 (MQ=255)

                                       cTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACGTCGGCTTTTGCCGCAACATGCAAAATGGTGGTCGGTTTGAt                                                     >  2:3895904/1‑93 (MQ=255)

                                       cTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACATCGGCTTTTGCCGCAACATGCAAAATGGTGGTCGGTTTGAt                                                     <  1:6288216/93‑1 (MQ=255)

                                        tGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACGTCGGCTTTTGCCGCAACATGCAAAATGGTGGTCGGTTTGAtt                                                    <  2:3289223/93‑1 (MQ=255)

                                          ccATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACGTCGGCTTTTGCCGCAACATGCAAAATGGTGGTCGGTTTGATTTc                                                  <  1:3686847/93‑1 (MQ=255)

                                          ccATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACGTCGGCTTTTGCCGCAACATGCAAAATGGTGGTCGGTTTGATTTc                                                  <  1:4096457/93‑1 (MQ=255)

                                          ccATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACGTCGGCTTTTGCCGCAACATGCAAAATGGTGGTCGGTTTGATTTc                                                  >  1:5777287/1‑93 (MQ=255)

                                           cATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACGTCGGCTTTTGCCGCAACATGCAAAATGGTGGTCGGTTTGATTTCa                                                 >  1:7096102/1‑93 (MQ=255)

                                           cATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACGTCGGCTTTTGCCGCAACATGCAAAATGGTGGTCGGTTTGATTTCa                                                 <  1:846676/93‑1 (MQ=255)

                                            aTTGCGCTAACGCCCGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACGTCGGCTTTTGCCGCAACATGCAAAATGGTGGTCGGTTTGATTTCAc                                                <  1:2838498/93‑1 (MQ=255)

                                            aTTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACGTCGGCTATTGCCGCAACATGCAAAATGGTGGTCGGTTTGATTTCAc                                                >  2:4317345/1‑93 (MQ=255)

                                             ttGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACGTCGGCTTTTGCCGCAACATGCAAAATGGTGGTCGGTTTGATTTCAcg                                               >  1:654878/1‑93 (MQ=255)

                                              tGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACGTCGGCTTTTGCCGCAACATGCAAAATGGTGGTCGGTTTGATTTCAcgc                                              <  2:3215505/93‑1 (MQ=255)

                                              tGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACGTCGGCTTTTGCCGCAACATGCAAAATGGTGGTCGGTTTGATTTCAcgc                                              <  2:1807702/93‑1 (MQ=255)

                                               gcgcTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACGTCGGCTTTTGCCGCAACATGCAAAATGGTGGTCGGTTTGATTTCAcgcg                                             >  2:7107086/1‑93 (MQ=255)

                                                cgcTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACGTCGGCTTTTGCCGCAACATGCAAAATGGTGGTCGGTTTGATTTCACgcgc                                            <  2:1916435/93‑1 (MQ=255)

                                                 gcTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACGTCGGCTTTTGCCGCAACATGCAAAATGGTGGTCGGTTTGATTTCACGCGcc                                           <  2:7525223/93‑1 (MQ=255)

                                                  cTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACGTCGGCTTTTGCCGCAACATGCAAAATGGTGGTCGGTTTGATTTCACGCGCCg                                          >  2:4214743/1‑93 (MQ=255)

                                                   tAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACGTCGGCTTTTGCCGCAACATGCAAAATGGTGGTCGGTTTGATTTCACGCGCCgg                                         >  1:452110/1‑93 (MQ=255)

                                                    aaCGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACGTCGGCTTTTGCCGCAACATGCAAAATGGTGGTCGGTTTGATTTCACGCGCCGGa                                        <  2:2417264/93‑1 (MQ=255)

                                                     aCGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACGTCGGCTTTTGCCGCAACATGCAAAATGGTGGTCGGTTTGATTTCACGCGCCGGAt                                       <  2:1252698/93‑1 (MQ=255)

                                                     aCGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACGTCGGCTTTTGCCGCAACATGCAAAATGGTGGTCGGTTTGATTTCACGCGCCGGAt                                       <  1:1080908/93‑1 (MQ=255)

                                                     aCGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACGTCGGCTTTTGCCGCAACATGCAAAATGGTGGTCGGTTTGATTTCACGCGCCGGAt                                       <  1:4651420/93‑1 (MQ=255)

                                                        ccAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACGTCGGCTTTTGCCGCAACATGCAAAATGGTGGTCGGTTTGATTTCACGCGCCGGATTGc                                    >  2:1602133/1‑93 (MQ=255)

                                                        ccAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACGTCGGCTTTTGCCGCAACATGCAAAATGGTGGTCGGTTTGATTTCACGCGCCGGATTGc                                    >  2:6305596/1‑93 (MQ=255)

                                                        ccAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACGTCGGCTTTTGCCGCAACATGCAAAATGGTGGTCGGTTTGATTTCACGCGCCGGATTGc                                    >  1:6088596/1‑93 (MQ=255)

                                                        ccAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACATCGGCTTTTGCCGCAACATGCAAAATGGTGGTCGGTTTGATTTCACGCGCCGGATTGc                                    >  2:3118864/1‑93 (MQ=255)

                                                          aGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACATCGGCTTTTGCCGCAACATGCAAAATGGTGGTCGGTTTGATTTCACGCGCCGGATTGccc                                  <  1:6882604/93‑1 (MQ=255)

                                                           gCTCGAGCGCCGCTTCCAGCCCCTGTTCCACGTCGGCTTTTGCCGCAACATGCAAAATGGTGGTCGGTTTGATTTCACGCGCCGGATTGCCCt                                 >  2:3601016/1‑93 (MQ=255)

                                                             tCGAGCGCCGCTTCCAGCCCCTGTTCCACGTCGGCTTTTGCCGCAACATGCAAAATGGTGGTCGGTTTGATTTCACGCGCCGGATTGCCCTgc                               >  2:1787537/1‑93 (MQ=255)

                                                                 gcgcCGCTTCCAGCCCCTGTTCCACGTCGGCTTTTGCCGCAACATGCAAAATGGTGGTCGGTTTGATTTCACGCGCCGGATTGCCCTGCGCCa                           >  1:724400/1‑93 (MQ=255)

                                                                    ccgcTTCCAGCCCCTGTTCCACGTCGGCTTTTGCCGCAACATGCAAAATGGTGGTCGGTTTGATTTCACGCGCCGGATTGCCCTGCGCCAgat                        <  1:3485195/93‑1 (MQ=255)

                                                                       cTTCCAGCCCCTGTTCCACGTCGGCTTTTGCCGCAACATGCAAAATGGTGGTCGGTTTGATTTCACGCGCCGGATTGCCCTGCGCCAgatgat                     <  1:4907670/93‑1 (MQ=255)

                                                                       cTTCCAGCCCCTGTTCCACGTCGGCTTTTGCCGCAACATGCAAAATGGTGGTCGGTTTGATTTCACGCGCCGGATTGCCCTGCGCCAgatgat                     <  2:625457/93‑1 (MQ=255)

                                                                        ttCCAGCCCCTGTTCCACGTCGGCTTTTGCCGCAACATGCAAAATGGTGGTCGGTTTGATTTCACGCGCCGGATTGCCCTGCGCCAGATGATa                    >  2:5972158/1‑93 (MQ=255)

                                                                         tCCAGCCCCTGTTCCACGTCGGCTTTTGCCGCAACATGCAAAATGGTGGTCGGTTTGATTTCACGCGCCGGATTGCCCTGCGCCAGATGATAg                   >  2:56073/1‑93 (MQ=255)

                                                                         tCCAGCCCCTGTTCCACGTCGGCTTTTGCCGCAACATGCAAAATGGTGGTCGGTTTGATTTCACGCGCCGGATTGCCCTGCGCCAGATGATAg                   <  2:2231288/93‑1 (MQ=255)

                                                                          ccAGCCCCTGTTCCACGTCGGCTTTTGCCGCAACATGCAAAATGGTGGTCGGTTTGATTTCACGCGCCGGATTGCCCTGCGCCAGATGATAGc                  <  1:2508090/93‑1 (MQ=255)

                                                                           cAGCCCCTGTTCCACATCGGCTTTTGCCGCAACATGCAAAATGGTGGTCGGTTTGATTTCACGCGCCGGATTGCCCTGCGCCAGATGATAGcc                 <  1:549575/93‑1 (MQ=255)

                                                                            acccccTGTTCCACATCGGCTTTTGCCGCAACATGCAAAAGGGTTGTCGGTTTGATTTCACGCGCCGGATTGCCCTGCGCCAGATGATAGCCa                <  1:2582578/91‑1 (MQ=255)

                                                                             gCCCCTGTTCCACGTCGGCTTTTGCCGCAACATGCAAAATGGTGGTCGGTTTGATTTCACGCGCCGGATTGCCCTGCGCCAGATGATAGCCAc               <  1:776255/93‑1 (MQ=255)

                                                                             gCCCCTGTTCCACGTCGGCTTTTGCCGCAACATGCAAAATGGTGGTCGGTTTGATTTCACGCGCCGGATTGCCCTGCGCCAGATGATAGCCAc               <  2:6836055/93‑1 (MQ=255)

                                                                             gCCCCTGTTCCACATCGGCTTTTGCCGCAACATGCAAAATGGTGGTCGGTTTGATTTCACGCGCCGGATTGCCCTGCGCCAGATGATAGCCAc               >  1:5241847/1‑93 (MQ=255)

                                                                              ccccTGTTCCACGTCGGCTTTTGCCGCAACATGCAAAATGGTGGTCGGTTTGATTTCACGCGCCGGATTGCCCTGCGCCAGATGATAGCCAcg              <  1:4338918/93‑1 (MQ=255)

                                                                               cccTGTTCCACGTCGGCTTTTGCCGCAACATGCAAAATGGTGGTCGGTTTGATTTCACGCGCCGGATTGCCCTGCGCCAGATGATAGCCAcgc             >  2:6900786/1‑93 (MQ=255)

                                                                                ccTGTTCCACGTCGGCTTTTGCCGCAACATGCAAAATGGTGGTCGGTTTGATTTCACGCGCCGGATTGCCCTGCGCCAGATGATAGCCAcgcg            >  1:1988694/1‑93 (MQ=255)

                                                                                     tCCACGTCGGCTTTTGCCGCAACATGCAAAATGGTGGTCGGTTTGATTTCACGCGCCGGATTGCCCTGCGCCAGATGATAGCCACGCGCCGCt       <  1:3516893/93‑1 (MQ=255)

                                                                                     tCCACGTCGGCTTTTGCCGCAACATGCAAAATGGTGGTCGGTTTGATTTCACGCGCCGGATTGCCCTGCGCCAGATGATAGCCACGCGCCGCt       >  2:3988172/1‑93 (MQ=255)

                                                                                       cacgTCGGCTTTTGCCGCAACATGCAAAATGGTGGTCGGTTTGATTTCACGCGCCGGATTGCCCTGCGCCAGATGATAGCCACGCGCCGCttt     <  2:5171074/93‑1 (MQ=255)

CGCCGCATCGTTGCCGCGTACCCACAGTTCTTCATGATACTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACATCGGCTTTTGCCGCAACATGCAAAATGGTGGTCGGTTTGATTTCACGCGCCGGATTGCCCTGCGCCAGATGATAGCCACGCGCCGCTTTGCT  >  NC_000913/3197857‑3198039

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

TTCGCCGCATCGTTGCCGCGTACCCACAGTTCTTCATGATACTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACATCGGCTTTTGCCGCAACATGCAAAATGGTGGTCGGTTTGATTTCACGCGCCGGATTGCCCTGCGCCAGATGATAGCCACGCGCCGCTTTGCTC  >  NC_000913/3197855‑3198040

TTCGCCGCATCGTTGCCGCGTACCCACAGTTCTTCATGATACTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACGT                                                                                              <  SRR4302542.2427519/94‑1 (MQ=60)

  CGCCGCATCGTTGCCGCGTACCCACAGTTCTTCATGATACTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACGTCG                                                                                            <  SRR4302542.1345484/94‑1 (MQ=60)

  CGCCGCATCGTTGCCGCGTACCCACAGTTCTTCATGATACTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACGTCG                                                                                            <  SRR4302542.5604409/94‑1 (MQ=60)

  CGCCGCATCGTTGCCGCGTACCCACAGTTCTTCATGATACTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACGTCG                                                                                            >  SRR4302542.1651626/1‑94 (MQ=60)

  CGCCGCATCGTTGCCGCGTACCCACAGTTCTTCATGATACTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACGTCG                                                                                            <  SRR4302542.6844116/94‑1 (MQ=60)

    CCGCATCGTTGCCGCGTACCCACAGTTCTTCATGATACTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACGTCGGC                                                                                          <  SRR4302542.748006/94‑1 (MQ=60)

      GCATCGTTGCCGCGTACCCACAGTTCTTCATGATACTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACGTCGGCTT                                                                                        >  SRR4302542.1210523/1‑94 (MQ=60)

      GCATCGTTGCCGCGTACCCACAGTTCTTCATGATACTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACGTCGGCTT                                                                                        >  SRR4302542.7129816/1‑94 (MQ=60)

      GCATCGTTGCCGCGTACCCACAGTTCTTCATGATACTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACGTCGGCTT                                                                                        >  SRR4302542.6735050/1‑94 (MQ=60)

      GCATCGTTGCCGCGTACCCACAGTTCTTCATGATACTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACATCGGCTT                                                                                        <  SRR4302542.5942345/94‑1 (MQ=60)

       CATCGTTGCCGCGTCCCCACAGTTCTTCATGATACTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACGTCGGCTTT                                                                                       <  SRR4302542.5588770/94‑1 (MQ=60)

       CATCGTTGCCGCGTACCCACAGTTCTTCATGATACTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACGTCGGCTTT                                                                                       >  SRR4302542.5057465/1‑94 (MQ=60)

       CATCGTTGCCGCGTACCCACAGTTCTTCATGATACTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACGTCGGCTTT                                                                                       <  SRR4302542.6713740/94‑1 (MQ=60)

       CATCGTTGCCGCGTACCCACAGTTCTTCATGATACTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACGTCGGCTTT                                                                                       >  SRR4302542.5223656/1‑94 (MQ=60)

        ATCGTTGCCGCGTACCCACAGTTCTTCATGATACTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACGTCGGCTTTT                                                                                      >  SRR4302542.1854474/1‑94 (MQ=60)

        ATCGTTGCCGCGTACCCACAGTTCTTCATGATACTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACGTCGGCTTTT                                                                                      <  SRR4302542.5732638/94‑1 (MQ=60)

         TCGTTGCCGCGTACCCACAGTTCTTCATGATACTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACGTCGGCTTTTG                                                                                     >  SRR4302542.3775945/1‑94 (MQ=60)

         TCGTTGCCGCGTACCCACAGTTCTTCATGATACTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACGTCGGCTTTTG                                                                                     <  SRR4302542.3130020/94‑1 (MQ=60)

         TCGTTGCCGCGTACCCACAGTTCTTCATGATACTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCAACGTCGGCTTTTG                                                                                     <  SRR4302542.1614497/94‑1 (MQ=60)

          CGTTGCCGCGTACCCACAGTTCTTCATGATACTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACGTCGGCTTTTGC                                                                                    <  SRR4302542.6306553/94‑1 (MQ=60)

          CGTTGCCGCGTACCCACAGTTCTTCATGATACTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACGTCGGCTTTTGC                                                                                    >  SRR4302542.3662858/1‑94 (MQ=60)

           GTTGCCGCGTACCCACAGTTCTTCATGATACTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACGTCGGCTTTTGCC                                                                                   >  SRR4302542.6689122/1‑94 (MQ=60)

           GTTGCCGCGTACCCACAGTTCTTCATGATACTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACGTCGGCTTTTGCC                                                                                   <  SRR4302542.3785705/94‑1 (MQ=60)

              GCCGCGTACCCACAGTTCTTCATGATACTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACGTCGGCTTTTGCCGCA                                                                                <  SRR4302542.5293212/94‑1 (MQ=60)

               CCGCGTACCCACAGTTCTTCATGATACTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACGTCGGCTTTTGCCGCAA                                                                               >  SRR4302542.1009460/1‑94 (MQ=60)

               CCGCGTACCCACAGTTCTTCATGATACTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACGTCGGCTTTTGCCGCAA                                                                               >  SRR4302542.934258/1‑94 (MQ=60)

                CGCGTACCCACAGTTCTTCATGATACTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACGTCGGCTTTTGCCGCAAC                                                                              >  SRR4302542.7396215/1‑94 (MQ=60)

                CGCGTACCCACAGTTCTTCATGATACTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACGTCGGCTTTTGCCGCAAC                                                                              <  SRR4302542.4355122/94‑1 (MQ=60)

                 GCGTACCCACAGTTCTTCATGATACTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACGTCGGCTTTTGCCGCAACA                                                                             <  SRR4302542.6265039/94‑1 (MQ=60)

                  CGTACCCACAGTTCTTCATGATACTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACGTCGGCTTTTGCCGCAACAT                                                                            >  SRR4302542.766877/1‑94 (MQ=60)

                  CGTACCCACAGTTCTTCATGATACTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACATCGGCTTTTGCCGCAACAT                                                                            <  SRR4302542.6172213/94‑1 (MQ=60)

                   GTACCCACAGTTCTTCATGATACTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACGTCGGCTTTTGCCGCAACATG                                                                           <  SRR4302542.5444665/94‑1 (MQ=60)

                    TACCCACAGTTCTTCATGATACTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACGTCGGCTTTTGCCGCAACATGC                                                                          <  SRR4302542.1565123/94‑1 (MQ=60)

                    TACCCACAGTTCTTCATGATACTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACGTCGGCTTTTGCCGCAACATGC                                                                          >  SRR4302542.647938/1‑94 (MQ=60)

                     ACCCACAGTTCTTCATGATACTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACGTCGGCTTTTGCCGCAACATGCA                                                                         <  SRR4302542.1888041/94‑1 (MQ=60)

                     ACCCACAGTTCTTCATGATACTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACATCGGCTTTTGCCGCAACATGCA                                                                         >  SRR4302542.6739483/1‑94 (MQ=60)

                      CCCACAGTTCTTCATGATACTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACGTCGGCTTTTGCCGCAACATGCAA                                                                        >  SRR4302542.4284164/1‑94 (MQ=60)

                      CCCACAGTTCTTCATGATACTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACGTCGGCTTTTGCCGCAACATGCAA                                                                        >  SRR4302542.5435534/1‑94 (MQ=60)

                      CCCACAGTTCTTCATGATACTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACGTCGGCTTTTGCCGCAACATGCAA                                                                        <  SRR4302542.3521081/94‑1 (MQ=60)

                      CCCACAGTTCTTCATGATACTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACGTCGGCTTTTGCCGCAACATGCAA                                                                        >  SRR4302542.491335/1‑94 (MQ=60)

                      CCCACAGTTCTTCATGATACTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACGTCGGCTTTTGCCGCAACATGCAA                                                                        >  SRR4302542.5016116/1‑94 (MQ=60)

                       CCACAGTTCTTCATGATACTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACGTCGGCTTTTGCCGCAACATGCAAA                                                                       >  SRR4302542.2318904/1‑94 (MQ=60)

                        CACAGTTCTTCATGATACTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACGTCGGCTTTTGCCGCAACATGCAAAA                                                                      >  SRR4302542.6344531/1‑94 (MQ=60)

                        CACAGTTCTTCATGATACTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACGTCGGCTTTTGCCGCAACATGCAAAA                                                                      <  SRR4302542.4047718/94‑1 (MQ=60)

                         ACAGTTCTTCATGATACTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACGTCGGCTTTTGCCGCAACATGCAAAAT                                                                     <  SRR4302542.5148255/94‑1 (MQ=60)

                         ACAGTTCTTCATGATACTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACGTCGGCTTTTGCCGCAACATGCAAAAT                                                                     <  SRR4302542.3201723/94‑1 (MQ=60)

                          CAGTTCTTCATGATACTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACGTCGGCTTTTGCCGCAACATGCAAAATG                                                                    >  SRR4302542.7023570/1‑94 (MQ=60)

                          CAGTTCTTCATGATACTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACGTCGGCTTTTGCCGCAACATGCAAAATG                                                                    <  SRR4302542.4683796/94‑1 (MQ=60)

                            GTTCTTCATGATACTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACGTCGGCTTTTGCCGCAACATGCAAAATGGT                                                                  >  SRR4302542.5482240/1‑94 (MQ=60)

                            GTTCTTCATGATACTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACGTCGGCTTTTGCCGCAACATGCAAAATGGT                                                                  <  SRR4302542.504810/94‑1 (MQ=60)

                             TTCTTCATGATACTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACGTCGGCTTTTGCCGCAACATGCAAAATGGTG                                                                 <  SRR4302542.7245187/94‑1 (MQ=60)

                              TCTTCATGATACTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACGTCGGCTTTTGCCGCAACATGCAAAATGGTGG                                                                <  SRR4302542.5846566/94‑1 (MQ=60)

                               CTTCATGATACTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACGTCGGCTTTTGCCGCAACATGCAAAATGGTGGT                                                               <  SRR4302542.7187038/94‑1 (MQ=60)

                               CTTCATGATACTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACGTCGGCTTTTGCCGCAACATGCAAAATGGTGGT                                                               <  SRR4302542.5403937/94‑1 (MQ=60)

                                TTCATGATACTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACGTCGGCTTTTGCCGCAACATGCAAAATGGTGGTC                                                              <  SRR4302542.5934092/94‑1 (MQ=60)

                                TTCATGATACTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACGTCGGCTTTTGCCGCAACATGCAAAATGGTGGTC                                                              <  SRR4302542.3019065/94‑1 (MQ=60)

                                 TCATGATACTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACGTCGGCTTTTGCCGCAACATGCAAAATGGTGGTCG                                                             <  SRR4302542.4453624/94‑1 (MQ=60)

                                 TCATGATACTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACGTCGGCTTTTGCCGCAACATGCAAAATGGTGGTCG                                                             <  SRR4302542.1292118/94‑1 (MQ=60)

                                 TCATGATACTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACGTCGGCTTTTGCCGCAACATGCAAAATGGTGGTCG                                                             <  SRR4302542.7083679/94‑1 (MQ=60)

                                  CATGATACTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACGTCGGCTTTTGCCGCAACATGCAAAATGGTGGTCGG                                                            <  SRR4302542.1073839/94‑1 (MQ=60)

                                  CATGATACTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACGTCGGCTTTTGCCGCAACATGCAAAATGGTGGTCGG                                                            <  SRR4302542.818140/94‑1 (MQ=60)

                                   ATGATACTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACGTCGGCTTTTGCCGCAACATGCAAAATGGTGGTCGGT                                                           >  SRR4302542.5716778/1‑94 (MQ=60)

                                    TGATACTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACGTCGGCTTTTGCCGCAACATGCAAAATGGTGGTCGGTT                                                          >  SRR4302542.3844402/1‑94 (MQ=60)

                                      ATACTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACGTCGGCTTTTGCCGCAACATGCAAAATGGTGGTCGGTTTG                                                        <  SRR4302542.3103535/94‑1 (MQ=60)

                                      ATACTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACGTCGGCTTTTGCCGCAACATGCAAAATGGTGGTCGGTTTG                                                        >  SRR4302542.2162657/1‑94 (MQ=60)

                                      ATACTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACGTCGGCTTTTGCCGCAACATGCAAAATGGTGGTCGGTTTG                                                        <  SRR4302542.368956/94‑1 (MQ=60)

                                        ACTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACGTCGGCTTTTGCCGCAACATGCAAAATGGTGGTCGGTTTGAT                                                      >  SRR4302542.711404/1‑94 (MQ=60)

                                         CTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACATCGGCTTTTGCCGCAACATGCAAAATGGTGGTCGGTTTGATT                                                     <  SRR4302542.6350175/94‑1 (MQ=60)

                                           GCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACGTCGGCTTTTGCCGCAACATGCAAAATGGTGGTCGGTTTGATTTC                                                   >  SRR4302542.5833373/1‑94 (MQ=60)

                                            CCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACGTCGGCTTTTGCCGCAACATGCAAAATGGTGGTCGGTTTGATTTCA                                                  <  SRR4302542.5040754/94‑1 (MQ=60)

                                            CCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACGTCGGCTTTTGCCGCAACATGCAAAATGGTGGTCGGTTTGATTTCA                                                  <  SRR4302542.3723224/94‑1 (MQ=60)

                                            CCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACGTCGGCTTTTGCCGCAACATGCAAAATGGTGGTCGGTTTGATTTCA                                                  <  SRR4302542.4136865/94‑1 (MQ=60)

                                            CCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACGTCGGCTTTTGCCGCAACATGCAAAATGGTGGTCGGTTTGATTTCA                                                  >  SRR4302542.7165722/1‑94 (MQ=60)

                                             CATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACGTCGGCTTTTGCCGCAACATGCAAAATGGTGGTCGGTTTGATTTCAC                                                 >  SRR4302542.6357504/1‑94 (MQ=60)

                                             CATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACGTCGGCTTTTGCCGCAACATGCAAAATGGTGGTCGGTTTGATTTCAC                                                 <  SRR4302542.5239771/94‑1 (MQ=60)

                                             CATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACGTCGGCTTTTGCCGCAACATGCAAAATGGTGGTCGGTTTGATTTCAC                                                 >  SRR4302542.5290744/1‑94 (MQ=60)

                                             CATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACGTCGGCTTTTGCCGCAACATGCAAAATGGTGGTCGGTTTGATTTCAC                                                 <  SRR4302542.853973/94‑1 (MQ=60)

                                              atggcgcgacCGCCAGCGCGCGCGCCGCTCCCAGCCCCGGTTCTACATCGGCTTTTGCCGCAACAGGCAAAATGGTGGTCGGTTTGTTTTCACG                                                <  SRR4302542.4336456/84‑1 (MQ=58)

                                              ATTGCGCTAACGCCCGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACGTCGGCTTTTGCCGCAACATGCAAAATGGTGGTCGGTTTGATTTCACG                                                <  SRR4302542.2865777/94‑1 (MQ=60)

                                              ATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACGTCGGCTTTTGCCGCAACATGCAAAATGGTGGTCGGTTTGATTTCACG                                                <  SRR4302542.7136948/94‑1 (MQ=60)

                                              ATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACGTCGGCTTTTGCCGCAACATGCAAAATGGTGGTCGGTTTGATTTCACG                                                >  SRR4302542.660531/1‑94 (MQ=60)

                                                TGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACGTCGGCTTTTGCCGCAACATGCAAAATGGTGGTCGGTTTGATTTCACGCG                                              <  SRR4302542.6962324/94‑1 (MQ=60)

                                                TGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACGTCGGCTTTTGCCGCAACATGCAAAATGGTGGTCGGTTTGATTTCACGCG                                              <  SRR4302542.7553029/94‑1 (MQ=60)

                                                    CTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACGTCGGCTTTTGCCGCAACATGCAAAATGGTGGTCGGTTTGATTTCACGCGCCGG                                          >  SRR4302542.456015/1‑94 (MQ=60)

                                                    CTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACATCGGCTTTTGCCGCAACATGCAAAATGGTGGTCGGTTTGATTTCACGCGCCGG                                          >  SRR4302542.5201033/1‑94 (MQ=60)

                                                     TAACGCCAGCTCGAGCGCCGCTTTCAGCCCCTGTTTAACGCCAGCTTTTGCCGCAACATGCAAAATAGAGATCGGCTTGATTTCACGCGCCGGA                                         >  SRR4302542.5760030/1‑94 (MQ=42)

                                                     TAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACGTCGGCTTTTGCCGCAACATGCAAAATGGTGGTCGGTTTGATTTCACGCGCCGGA                                         >  SRR4302542.3646677/1‑94 (MQ=60)

                                                      AACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACGTCGGCTTTTGCCGCAACATGCAAAATGGTGGTCGGTTTGATTTCACGCGCCGGAT                                        <  SRR4302542.170872/94‑1 (MQ=60)

                                                       ACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACGTCGGCTTTTGCCGCAACATGCAAAATGGTGGTCGGTTTGATTTCACGCGCCGGATT                                       <  SRR4302542.1090186/94‑1 (MQ=60)

                                                       ACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACGTCGGCTTTTGCCGCAACATGCAAAATGGTGGTCGGTTTGATTTCACGCGCCGGATT                                       <  SRR4302542.4696894/94‑1 (MQ=60)

                                                       ACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACATCGGCTTTTGCCGCAACATGCAAAATGGTGGTCGGTTTGATTTCACGCGCCGGATT                                       <  SRR4302542.2267583/94‑1 (MQ=60)

                                                        CGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACGTCGGCTTTTGCCGCAACATGCAAAATGGTGGTCGGTTTGATTTCACGCGCCGGATTG                                      <  SRR4302542.7477909/94‑1 (MQ=60)

                                                        CGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACATCGGCTTTTGCCGCAACATGCAAAATGGTGGTCGGTTTGATTTCACGCGCCGGATTG                                      <  SRR4302542.2367063/94‑1 (MQ=60)

                                                         GCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACGTCGGCTTTTGCCGCAACATGCAAAATGGTGGTCGGTTTGATTTCACGCGCCGGATTGC                                     >  SRR4302542.6147549/1‑94 (MQ=60)

                                                          CCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACGTCGGCTTTTGCCGCAACATGCAAAATGGTGGTCGGTTTGATTTCACGCGCCGGATTGCC                                    >  SRR4302542.3307456/1‑94 (MQ=60)

                                                          CCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACGTCGGCTTTTGCCGCAACATGCAAAATGGTGGTCGGTTTGATTTCACGCGCCGGATTGCC                                    <  SRR4302542.3639702/94‑1 (MQ=60)

                                                            AGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACGTCGGCTTTTGCCGCATCATGCAAAATGGTGGTCGGTTTGATTTCACGCGCCGGATTGCCCT                                  <  SRR4302542.6748663/94‑1 (MQ=60)

                                                            AGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACATCGGCTTTTGCCGCAACATGCAAAATGGTGGTCGGTTTGATTTCACGCGCCGGATTGCCCT                                  <  SRR4302542.6950210/94‑1 (MQ=60)

                                                            AGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACATCGGCTTTTGCCGCAACATGCAAAATGGTGGTCGGTTTGATTTCACGCGCCGGATTCCCCT                                  <  SRR4302542.35173/94‑1 (MQ=60)

                                                             GCTCGAGCGCCGCTTCCAGCCCCTGTTCCACGTCGGCTTTTGCCGCAACATGCAAAATGGTGGTCGGTTTGATTTCACGCGCCGGATTGCCCTG                                 <  SRR4302542.5793265/94‑1 (MQ=60)

                                                                  AGCGCCGCTTCCAGCCCCTGTTCCACGTCGGCTTTTGCCGCAACATGCAAAATGGTGGTCGGTTTGATTTCACGCGCCGGATTGCCCTGCGCCA                            >  SRR4302542.730641/1‑94 (MQ=60)

                                                                     GCCGCTTCCAGCCCCTGTTCCACATCGGCTTTTGCCGCAACATGCAAAATGGTGGTCGGTTTGATTTCACGCGCCGGATTGCCCTGCGCCAGAT                         >  SRR4302542.4072194/1‑94 (MQ=60)

                                                                      CCGCTTCCAGCCCCTGTTCCACGTCGGCTTTTGCCGCAACATGCAAAATGGTGGTCGGTTTGATTTCACGCGCCGGATTGCCCTGCGCCAGATG                        <  SRR4302542.4572411/94‑1 (MQ=60)

                                                                      CCGCTTCCAGCCCCTGTTCCACGTCGGCTTTTGCCGCAACATGCAAAATGGTGGTCGGTTTGATTTCACGCGCCGGATTGCCCTGCGCCAGATG                        <  SRR4302542.3517799/94‑1 (MQ=60)

                                                                      CCGCTTCCAGCCCCTGTTCCACATCGGCTTTTGCCGCAACATGCAAAATGGTGGTCGGTTTGATTTCACGCGCCGGATTGCCCTGCGCCAGATG                        <  SRR4302542.3986782/94‑1 (MQ=60)

                                                                        GCTTCCAGCCCCTGTTCCACGTCGGCTTTTGCCGCAACATGCAAAATGGTGGTCGGTTTGATTTCACGCGCCGGATTGCCCTGCGCCAGATGAT                      <  SRR4302542.3157432/94‑1 (MQ=60)

                                                                         CTTCCAGCCCCTGTTCCACGTCGGCTTTTGCCGCAACATGCAAAATGGTGGTCGGTTTGATTTCACGCGCCGGATTGCCCTGCGCCAGATGATA                     <  SRR4302542.4955403/94‑1 (MQ=60)

                                                                         CTTCCAGCCCCTGTTCCACGTCGGCTTTTGCCGCAACATGCAAAATGGTGGTCGGTTTGATTTCACGCGCCGGATTGCCCTGCGCCAGATGATA                     >  SRR4302542.7183473/1‑94 (MQ=60)

                                                                         CTTCCAGCCCCTGTTCCACGTCGGCTTTTGCCGCAACATGCAAAATGGTGGTCGGTTTGATTTCACGCGCCGGATTGCCCTGCGCCAGATGATA                     >  SRR4302542.2815543/1‑94 (MQ=60)

                                                                            CCAGCCCCTGTTCCACGTCGGCTTTTGCCGCAACATGCAAAATGGTGGTCGGTTTGATTTCACGCGCCGGATTGCCCTGCGCCAGATGATAGCC                  <  SRR4302542.2532431/94‑1 (MQ=60)

                                                                            CCAGCCCCTGTTCCACGTCGGCTTTTGCCGCAACATGCAAAATGGTGGTCGGTTTGATTTCACGCGCCGGATTGCCCTGCGCCAGATGATAGCC                  >  SRR4302542.6152359/1‑94 (MQ=60)

                                                                             CAGCCCCTGTTCCACATCGGCTTTTGCCGCAACATGCAAAATGGTGGTCGGTTTGATTTCACGCGCCGGATTGCCCTGCGCCAGATGATAGCCA                 <  SRR4302542.554317/94‑1 (MQ=60)

                                                                              AGCCCCTGTTCCACGTCGGCTTTTGCCGCAACATGCAAAATGGTGGTCGGTTTGATTTCACGCGCCGGATTGCCCTGCGCCAGATGATAGCCAC                >  SRR4302542.70912/1‑94 (MQ=60)

                                                                              AGCCCCTGTTCCACGTCGGCTTTTGCCGCAACATGCAAAATGGTGGGCGGTTTGATTTCACGCGCCGGATTGCCCTGCGCCAGATGATAGCCAC                <  SRR4302542.4114409/94‑1 (MQ=60)

                                                                              AGCCCCTGTTCCACATCGGCTTTTGCCGCAACATGCAAAATGGTGGTCGGTTTGATTTCACGCGCCGGATTGCCCTGCGCCAGATGATAGCCAC                <  SRR4302542.597877/94‑1 (MQ=60)

                                                                              AGCCCCTGTTCCACATCGGCTTTTGCCGCAACATGCAAAATGGTGGTCGGTTTGATTTCACGCGCCGGATTGCCCTGCGCCAGATGATAGCCAC                >  SRR4302542.5292804/1‑94 (MQ=60)

                                                                              AGCCCCTGTTCCACATCGGCTTTTGCCGCAACATGCAAAATGGTGGTCGGTTTGATTTCACGCGCCGGATTGCCCTGCGCCAGATGATAGCCAC                <  SRR4302542.194926/94‑1 (MQ=60)

                                                                              ACCCCCTGTTCCACATCGGCTTTTGCCGCAACATGCAAAAGGGTTGTCGGTTTGATTTCACGCGCCGGATTGCCCTGCGCCAGATGATAGCCAC                <  SRR4302542.2607607/94‑1 (MQ=60)

                                                                               GCCCCTGTTCCACGTCGGCTTTTGCCGCAACATGCAAAATGGTGGTCGGTTTGATTTCACGCGCCGGATTGCCCTGCGCCAGATGATAGCCACG               <  SRR4302542.782958/94‑1 (MQ=60)

                                                                                CCCCTGTTCCACGTCGGCTTTTGCCGCAACATGCAAAATGGTGGTCGGTTTGATTTCACGCGCCGGATTGCCCTGCGCCAGATGATAGCCACGC              >  SRR4302542.6736499/1‑94 (MQ=60)

                                                                                CCCCTGTTCCACGTCGGCTTTTGCCGCAACATGCAAAATGGTGGTCGGTTTGATTTCACGCGCCGGATTGCCCTGCGCCAGATGATAGCCACGC              <  SRR4302542.4381550/94‑1 (MQ=60)

                                                                                 CCCTGTTCCACGTCGGCTTTTGCCGCAACATGCAAAATGGTGGTCGGTTTGATTTCACGCGCCGGATTGCCCTGCGCCAGATGATAGCCACGCG             <  SRR4302542.871495/94‑1 (MQ=60)

                                                                                 CCCTGTTCCACGTCGGCTTTTGCCGCAACATGCAAAATGGTGGTCGGTTTGATTTCACGCGCCGGATTGCCCTGCGCCAGATGATAGCCACGCG             <  SRR4302542.836294/94‑1 (MQ=60)

                                                                                 CCCTGTTCCACGTCGGCTTTTGCCGCAACATGCAAAATGGTGGTCGGTTTGATTTCACGCGCCGGATTGCCCTGCGCCAGATGATAGCCACGCG             >  SRR4302542.2005848/1‑94 (MQ=60)

                                                                                  CCTGTTCCACGTCGGCTTTTGCCGCAACATGCAAAATGGTGGTCGGTTTGATTTCACGCGCCGGATTGCCCTGCGCCAGATGATAGCCACGCGC            >  SRR4302542.4416699/1‑94 (MQ=60)

                                                                                  CCTGTTCCACGTCGGCTTTTGCCGCAACATGCAAAATGGTGGTCGGTTTGATTTCACGCGCCGGATTGCCCTGCGCCAGATGATAGCCACGCGC            >  SRR4302542.3998657/1‑94 (MQ=60)

                                                                                  CCTGTTCCACGTCGGCTTTTGCCGCAACATGCAAAATGGTGGTCGGTTTGATTTCACGCGCCGGATTGCCCTGCGCCAGATGATAGCCACGCGC            >  SRR4302542.5664974/1‑94 (MQ=60)

                                                                                   CTGTTCCACGTCGGCTTTTGCCGCAACATGCAAAATGGTGGTCGGTTTGATTTCACGCGCCGGATTGCCCTGCGCCAGATGATAGCCACGCGCC           >  SRR4302542.2589484/1‑94 (MQ=60)

                                                                                       TCCACGTCGGCTTTTGCCGCAACATGCAAAATGGTGGTCGGTTTGATTTCACGCGCCGGATTGCCCTGCGCCAGATGATAGCCACGCGCCGCTT       <  SRR4302542.7285223/94‑1 (MQ=60)

                                                                                       TCCACGTCGGCTTTTGCCGCAACATGCAAAATGGTGGTCGGTTTGATTTCACGCGCCGGATTGCCCTGCGCCAGATGATAGCCACGCGCCGCTT       <  SRR4302542.3549771/94‑1 (MQ=60)

                                                                                        CCACGTCGGCTTTTGCCGCAACATGCAAAATGGTGGTCGGTTTGATTTCACGCGCCGGATTGCCCTGCGCCAGATGATAGCCACGCGCCGCTTT      >  SRR4302542.2360703/1‑94 (MQ=60)

                                                                                        CCACGTCGGCTTTTGCCGCAACATGCAAAATGGTGGTCGGTTTGATTTCACGCGCCGGATTGCCCTGCGCCAGATGATAGCCACGCGCCGCTTT      >  SRR4302542.2745963/1‑94 (MQ=60)

                                                                                        CCACGTCGGCTTTTGCCGCAACATGCAAAATGGTGGTCGGTTTGATTTCACGCGCCGGATTGCCCTGCGCCAGATGATAGCCACGCGCCGCTTT      >  SRR4302542.3036540/1‑94 (MQ=60)

                                                                                         CACATCGGCTTTTGCCGCAACATGCAAAATGGTGGTCGGTTTGATTTCACGCGCCGGATTGCCCTGCGCCAGATGATAGCCACGCGCCGCTTTG     <  SRR4302542.5122511/94‑1 (MQ=60)

                                                                                           CGTCGGCTTTTGCCGCAACATGCAAAATGGTGGTCGGTTTGATTTCACGCGCCGGATTGCCCTGCGCCAGATGATAGCCACGCGCCGCTTTGNN   >  SRR4302542.2161484/1‑94 (MQ=60)

                                                                                           CGTCGGCTTTTGCCGCAACATGCAAAATGGTGGTCGGTTTGATTTCACGCGCCGGATTGCCCTGCGCCAGATGATAGCCACGCGCCGCTTTGCT   >  SRR4302542.2736911/1‑94 (MQ=60)

                                                                                           CATCGGCTTTTGCCGCAACATGCAAAATGGTGGTCGGTTTGATTTCACGCGCCGGATTGCCCTGCGCCAGATGATAGCCACGCGCCGCTTTGCT   >  SRR4302542.4165186/1‑94 (MQ=60)

                                                                                            GTCGGCTTTTGCCGCAACATGCAAAATGGTGGTCGGTTTGATTTCACGCGCCGGATTGCCCTGCGCCAGATGATAGCCACGCGCCGCTTTGCTC  >  SRR4302542.3915420/1‑94 (MQ=60)

TTCGCCGCATCGTTGCCGCGTACCCACAGTTCTTCATGATACTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACATCGGCTTTTGCCGCAACATGCAAAATGGTGGTCGGTTTGATTTCACGCGCCGGATTGCCCTGCGCCAGATGATAGCCACGCGCCGCTTTGCTC  >  NC_000913/3197855‑3198040

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`