Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1061 294.1 15065038 98.6% 14854127 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,250,165 C→T R74R (CGC→CGT)  yhaH → putative cytochrome

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,250,1650CT88.5% 565.5 / 41.1 191R74R (CGC→CGT) yhaHputative cytochrome
Reads supporting (aligned to +/- strand):  ref base C (6/16);  new base T (45/124);  total (51/140)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.08e-01

Only 100 of 196 total aligned reads displayed.

TTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTATCATCGTCTTTAACTGCCAG  >  NC_000913/3250073‑3250255
                                                                                            |                                                                                          
ttAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCcgccgc                                                                                            >  1:2306553/1‑93 (MQ=255)
  aGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCt                                                                                          <  2:6143968/93‑1 (MQ=255)
     cTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCa                                                                                       <  2:6112054/93‑1 (MQ=255)
       ggCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACg                                                                                     >  1:3130486/1‑93 (MQ=255)
         cAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCATGac                                                                                   >  2:659016/1‑93 (MQ=255)
         cAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGac                                                                                   >  2:4826490/1‑93 (MQ=255)
          aaCGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGaca                                                                                  <  1:1713017/93‑1 (MQ=255)
          aaCGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGaca                                                                                  >  1:1935255/1‑93 (MQ=255)
           aCGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGacac                                                                                 >  1:1629215/1‑93 (MQ=255)
           aCGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGacac                                                                                 <  1:4729051/93‑1 (MQ=255)
           aCGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGacac                                                                                 <  1:4893655/93‑1 (MQ=255)
            cgcgCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCTGCCGTCTGCACGACacc                                                                                >  2:4263324/1‑93 (MQ=255)
              cgcTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACaccga                                                                              <  2:1819613/93‑1 (MQ=255)
               gcTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACaccgac                                                                             <  1:5987927/93‑1 (MQ=255)
               gcTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTGGTGTTCTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACaccgac                                                                             <  1:6298725/93‑1 (MQ=255)
                  ggtggCGAAGGCATCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGc                                                                          <  2:7388564/93‑1 (MQ=255)
                  ggtggCGAAGGCATCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGc                                                                          >  2:6952583/1‑93 (MQ=255)
                    tggCGAAGGCATCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTc                                                                        >  2:3676430/1‑93 (MQ=255)
                     ggCGAAGGCATCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCg                                                                       <  2:191673/93‑1 (MQ=255)
                       cGAAGGCATCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCGGc                                                                     <  2:7347272/93‑1 (MQ=255)
                       cGAAGGCATCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCGGc                                                                     >  1:7235017/1‑93 (MQ=255)
                         aaGGCATCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCgt                                                                   <  1:5815382/93‑1 (MQ=255)
                           ggCATCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCGGCgtgg                                                                 >  2:5003857/1‑93 (MQ=255)
                           ggCATCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCGGCgtgg                                                                 <  1:3714288/93‑1 (MQ=255)
                           ggCATCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCGGCgtgg                                                                 <  1:1311091/93‑1 (MQ=255)
                           ggCATCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCGGCgtgg                                                                 >  1:4131863/1‑93 (MQ=255)
                           ggCATCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCGGCgtgg                                                                 <  1:1156666/93‑1 (MQ=255)
                            gCATCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCGGCgtggt                                                                <  1:5028755/93‑1 (MQ=255)
                            gCATCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCGGCgtggt                                                                <  1:1108605/93‑1 (MQ=255)
                            gCATCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCGGCgtggt                                                                <  1:4365447/93‑1 (MQ=255)
                            gCATCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCGGCgtggt                                                                <  1:2541921/93‑1 (MQ=255)
                            gCATCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCGGCgtggt                                                                >  2:6194903/1‑93 (MQ=255)
                             cATCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCGGCgtggtg                                                               <  1:6462237/93‑1 (MQ=255)
                              aTCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCGGCGtggtgg                                                              >  2:1103608/1‑93 (MQ=255)
                               tCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCGGCGTGGTggg                                                             >  1:1213953/1‑93 (MQ=255)
                                ccTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCGGCGTGGTGGGc                                                            <  2:4201978/93‑1 (MQ=255)
                                 cTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCGGCGTGGTGGGCa                                                           <  2:5646643/93‑1 (MQ=255)
                                  tGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCGGCGTGGTGGGCAc                                                          <  1:4106830/93‑1 (MQ=255)
                                   gacgacAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCGGCGTGGTGGGCACt                                                         <  1:187526/93‑1 (MQ=255)
                                     cgacAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGc                                                       >  1:3848621/1‑93 (MQ=255)
                                      gacAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCt                                                      >  2:1445565/1‑93 (MQ=255)
                                      gacAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCt                                                      >  2:2306773/1‑93 (MQ=255)
                                       acAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTa                                                     <  2:4582025/93‑1 (MQ=255)
                                       acAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTa                                                     <  2:11913/93‑1 (MQ=255)
                                        cAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTAt                                                    <  2:3628369/93‑1 (MQ=255)
                                         aaTCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTAtt                                                   >  1:763473/1‑93 (MQ=255)
                                         aaTCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTAtt                                                   <  1:678541/93‑1 (MQ=255)
                                          aTCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTc                                                  <  1:948465/93‑1 (MQ=255)
                                           tCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCt                                                 >  1:3573972/1‑93 (MQ=255)
                                            cTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCtt                                                <  1:2552039/93‑1 (MQ=255)
                                             tACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTa                                               >  2:3457012/1‑93 (MQ=255)
                                              aCGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTaa                                              >  1:2085060/1‑93 (MQ=255)
                                              aCGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTaa                                              <  2:7413477/93‑1 (MQ=255)
                                               cGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAAt                                             <  2:4915792/93‑1 (MQ=255)
                                                  tATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATccc                                          >  2:553949/1‑93 (MQ=255)
                                                  tATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATccc                                          <  1:513193/93‑1 (MQ=255)
                                                   aTTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCg                                         >  2:703808/1‑93 (MQ=255)
                                                    ttCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGt                                        <  2:5327537/93‑1 (MQ=255)
                                                       tGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTa                                     <  1:3521447/93‑1 (MQ=255)
                                                       tGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTa                                     >  1:5449609/1‑93 (MQ=255)
                                                       tGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTa                                     <  2:4671444/93‑1 (MQ=255)
                                                        nntgtTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTAt                                    <  1:2868125/91‑1 (MQ=255)
                                                        ggTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTAt                                    <  1:4051949/93‑1 (MQ=255)
                                                         gtgtTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATc                                   >  1:2206766/1‑93 (MQ=255)
                                                          tgtTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCg                                  <  2:2428136/93‑1 (MQ=255)
                                                          tgtTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCg                                  >  2:6539759/1‑93 (MQ=255)
                                                          tgtTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCg                                  <  2:3189925/93‑1 (MQ=255)
                                                            tttttACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCggc                                <  1:87135/93‑1 (MQ=255)
                                                                 aCCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCggctggct                           >  2:2076220/1‑93 (MQ=255)
                                                                   cGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCtta                         <  2:2483913/93‑1 (MQ=255)
                                                                   cGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCtta                         <  2:3974584/93‑1 (MQ=255)
                                                                   cGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCtta                         >  2:6236874/1‑93 (MQ=255)
                                                                   cGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCtta                         <  1:5627263/93‑1 (MQ=255)
                                                                   cGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCtta                         <  2:1519187/93‑1 (MQ=255)
                                                                   cGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGGttt                         >  1:1387023/1‑92 (MQ=255)
                                                                   cGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCtta                         <  1:3899503/93‑1 (MQ=255)
                                                                   cGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCtta                         >  1:5827559/1‑93 (MQ=255)
                                                                    gtggtgGGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCttat                        <  2:5899539/93‑1 (MQ=255)
                                                                     tggtggGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCttatt                       <  2:720265/93‑1 (MQ=255)
                                                                      ggtggGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCttatta                      <  2:896783/93‑1 (MQ=255)
                                                                      ggtggGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCttatta                      <  2:4068884/93‑1 (MQ=255)
                                                                       gtggGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTtattat                     <  1:4262374/93‑1 (MQ=255)
                                                                       gtggGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTtattat                     <  1:65798/93‑1 (MQ=255)
                                                                        tggGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCGGGCTTATTatc                    <  2:4658466/93‑1 (MQ=255)
                                                                         gggCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTatca                   <  1:3323838/93‑1 (MQ=255)
                                                                           gCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTatcatc                 <  1:267775/93‑1 (MQ=255)
                                                                           gCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTatcatc                 <  2:2343956/93‑1 (MQ=255)
                                                                             ggTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTATCATCGt               >  2:4390398/1‑93 (MQ=255)
                                                                               ttCAGTTCCGCCGTCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTATCATCGTCt             <  2:2396002/93‑1 (MQ=255)
                                                                                 cAGTTCCGCCGTCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTATCATCGTCttt           <  2:2557114/93‑1 (MQ=255)
                                                                                  aGTTCCGCCGTCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTATCATCGTCTTTa          <  2:5955343/93‑1 (MQ=255)
                                                                                  aGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTATCATCGTCTTTa          <  1:4154790/93‑1 (MQ=255)
                                                                                   gTTCCGCCGTCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGGTTATTTTTATCGTCTTTaa         >  1:2607435/1‑93 (MQ=255)
                                                                                   gTTCCGCCGTCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTATCATCGTCTTTaa         <  1:5610986/93‑1 (MQ=255)
                                                                                   gTTCCGCCGTCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTATCATCGTCTTTaa         <  1:4079455/93‑1 (MQ=255)
                                                                                   gTTCCGCCGTCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTATCATCGTCTTTaa         <  2:1279528/93‑1 (MQ=255)
                                                                                   gTTCCGCCGTCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTATCATCGTCTTTaa         <  1:5884251/93‑1 (MQ=255)
                                                                                        gccgtcTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTATCATCGTCTTTAAATGcc    >  1:3505134/1‑93 (MQ=255)
                                                                                         ccgtcTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTATCATCGTCTTTAACTGCCa   <  1:7433691/93‑1 (MQ=255)
                                                                                          cgccTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTATCATCGTCTTTAACTGCCAg  <  2:2875901/93‑1 (MQ=255)
                                                                                            |                                                                                          
TTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTATCATCGTCTTTAACTGCCAG  >  NC_000913/3250073‑3250255

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTATCATCGTCTTTAACTGCCAGGCG  >  NC_000913/3250072‑3250258
                                                                                             |                                                                                             
GTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGC                                                                                               >  SRR4302542.2326362/1‑94 (MQ=60)
   AGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTG                                                                                            <  SRR4302542.1386938/94‑1 (MQ=60)
    GGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGC                                                                                           >  SRR4302542.5677385/1‑94 (MQ=60)
       TGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACG                                                                                        >  SRR4302542.3160256/1‑94 (MQ=60)
          CAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACA                                                                                     <  SRR4302542.4133259/94‑1 (MQ=60)
          CAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACA                                                                                     >  SRR4302542.1951966/1‑94 (MQ=60)
           AACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACAC                                                                                    >  SRR4302542.3696456/1‑94 (MQ=60)
           AACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACAC                                                                                    >  SRR4302542.4999291/1‑94 (MQ=60)
           AACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACAC                                                                                    >  SRR4302542.1643274/1‑94 (MQ=60)
           AACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACAC                                                                                    <  SRR4302542.1727897/94‑1 (MQ=60)
            ACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACACC                                                                                   <  SRR4302542.4775168/94‑1 (MQ=60)
            ACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACC                                                                                   <  SRR4302542.4941237/94‑1 (MQ=60)
                GCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACACCGACC                                                                               >  SRR4302542.3172534/1‑94 (MQ=60)
                GCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACACCGACC                                                                               <  SRR4302542.6045932/94‑1 (MQ=60)
                GCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTGGTGTTCTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACACCGACC                                                                               <  SRR4302542.6360777/94‑1 (MQ=60)
                 CTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCG                                                                              <  SRR4302542.2364784/94‑1 (MQ=60)
                 CTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCG                                                                              <  SRR4302542.3487960/94‑1 (MQ=60)
                     TGGCGAAGGCATCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCG                                                                          <  SRR4302542.3593820/94‑1 (MQ=60)
                       GCGAAGGCATCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCGGC                                                                        >  SRR4302542.7305989/1‑94 (MQ=60)
                       GCGAAGGCATCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCGGC                                                                        <  SRR4302542.2753694/94‑1 (MQ=60)
                          AAGGCATCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCGGCGTG                                                                     <  SRR4302542.4156890/94‑1 (MQ=60)
                          AAGGCATCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTG                                                                     <  SRR4302542.5871814/94‑1 (MQ=60)
                           AGGCATCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCGGCGTGG                                                                    >  SRR4302542.4172572/1‑94 (MQ=60)
                            GGCATCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCGGCGTGGT                                                                   <  SRR4302542.1322390/94‑1 (MQ=60)
                            GGCATCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCGGCGTGGT                                                                   <  SRR4302542.1166577/94‑1 (MQ=60)
                            GGCATCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCGGCGTGGT                                                                   <  SRR4302542.3751037/94‑1 (MQ=60)
                             GCATCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCCCGACACCGACCGCTCGGCGTGGTG                                                                  <  SRR4302542.4408301/94‑1 (MQ=60)
                             GCATCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCGGCGTGGTG                                                                  <  SRR4302542.1118084/94‑1 (MQ=60)
                             GCATCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCGGCGTGGTG                                                                  <  SRR4302542.5077572/94‑1 (MQ=60)
                             GCATCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCGGCGTGGTG                                                                  <  SRR4302542.4408302/94‑1 (MQ=60)
                             GCATCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCGGCGTGGTG                                                                  <  SRR4302542.2566562/94‑1 (MQ=60)
                              CATCCTGACGACAATCTACGGTGTTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCGGCGTGGTGG                                                                 <  SRR4302542.4568050/94‑1 (MQ=60)
                              CATCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCGGCGTGGTGG                                                                 <  SRR4302542.644867/94‑1 (MQ=60)
                              CATCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCGGCGTGGTGG                                                                 <  SRR4302542.6525905/94‑1 (MQ=60)
                              CATCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCGGCGTGGTGG                                                                 <  SRR4302542.1000314/94‑1 (MQ=60)
                               ATCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCGGCGTGGTGGG                                                                >  SRR4302542.1224397/1‑94 (MQ=60)
                               ATCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCGGCGTGGTGGG                                                                <  SRR4302542.3894024/94‑1 (MQ=60)
                               ATCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCGGCGTGGTGGG                                                                <  SRR4302542.4219505/94‑1 (MQ=60)
                               ATCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCGGCGTGGTGGG                                                                >  SRR4302542.2052004/1‑94 (MQ=60)
                               ATCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCGGCGTGGTGGG                                                                >  SRR4302542.6225300/1‑94 (MQ=60)
                               ATCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCGGCGTGGTGGG                                                                <  SRR4302542.7341058/94‑1 (MQ=60)
                               ATCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGATTCAGTTCCGCCGTCTGCACGACACCGACCGCTCGGCGTGGTGGG                                                                <  SRR4302542.5826667/94‑1 (MQ=60)
                                 CCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCGGCGTGGTGGGCA                                                              <  SRR4302542.1676299/94‑1 (MQ=60)
                                   TGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCGGCGTGGTGGGCACT                                                            <  SRR4302542.6732858/94‑1 (MQ=60)
                                   TGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCGGCGTGGTGGGCACT                                                            <  SRR4302542.4147320/94‑1 (MQ=60)
                                    GACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTG                                                           <  SRR4302542.189116/94‑1 (MQ=60)
                                     ACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGC                                                          >  SRR4302542.3886648/1‑94 (MQ=60)
                                        ACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTAT                                                       <  SRR4302542.1592823/94‑1 (MQ=60)
                                         CAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATT                                                      >  SRR4302542.770088/1‑94 (MQ=60)
                                          AATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTC                                                     <  SRR4302542.6555503/94‑1 (MQ=60)
                                          AATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTC                                                     <  SRR4302542.684401/94‑1 (MQ=60)
                                           ATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCT                                                    >  SRR4302542.858768/1‑94 (MQ=60)
                                           ATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCT                                                    <  SRR4302542.956618/94‑1 (MQ=60)
                                           ATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCT                                                    >  SRR4302542.3607331/1‑94 (MQ=60)
                                             CTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTA                                                  <  SRR4302542.2576775/94‑1 (MQ=60)
                                              TACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAA                                                 >  SRR4302542.2103060/1‑94 (MQ=60)
                                                CGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATC                                               <  SRR4302542.4934161/94‑1 (MQ=60)
                                                   TATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCG                                            <  SRR4302542.1305519/94‑1 (MQ=60)
                                                   TATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCG                                            <  SRR4302542.517646/94‑1 (MQ=60)
                                                     TTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTT                                          <  SRR4302542.5920709/94‑1 (MQ=60)
                                                       CTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTA                                        >  SRR4302542.5502556/1‑94 (MQ=60)
                                                        TGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTAT                                       <  SRR4302542.417115/94‑1 (MQ=60)
                                                        TGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTAT                                       <  SRR4302542.3554357/94‑1 (MQ=60)
                                                         NNTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATC                                      <  SRR4302542.2895677/94‑1 (MQ=60)
                                                         GGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATC                                      >  SRR4302542.2225750/1‑94 (MQ=60)
                                                         GGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATC                                      <  SRR4302542.4091910/94‑1 (MQ=60)
                                                          GTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCG                                     <  SRR4302542.6030814/94‑1 (MQ=60)
                                                          GTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCG                                     <  SRR4302542.7595916/94‑1 (MQ=60)
                                                          GTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCG                                     <  SRR4302542.2568812/94‑1 (MQ=60)
                                                            GTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGC                                   <  SRR4302542.3547831/94‑1 (MQ=60)
                                                             TTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCT                                  <  SRR4302542.87859/94‑1 (MQ=60)
                                                              TTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTG                                 <  SRR4302542.5478769/94‑1 (MQ=60)
                                                               TTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGG                                <  SRR4302542.2213629/94‑1 (MQ=60)
                                                               TTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGG                                <  SRR4302542.4026598/94‑1 (MQ=60)
                                                                TTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGG                               >  SRR4302542.5879456/1‑94 (MQ=60)
                                                                TTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGC                               <  SRR4302542.512369/94‑1 (MQ=60)
                                                                   CCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGgttt                            >  SRR4302542.1399006/1‑90 (MQ=60)
                                                                   CCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTA                            >  SRR4302542.5884125/1‑94 (MQ=60)
                                                                    CGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTAT                           <  SRR4302542.5681904/94‑1 (MQ=60)
                                                                    CGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTAT                           <  SRR4302542.3937996/94‑1 (MQ=60)
                                                                      TGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTA                         <  SRR4302542.2632750/94‑1 (MQ=60)
                                                                        GTGGGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTATC                       <  SRR4302542.4304337/94‑1 (MQ=60)
                                                                        GTGGGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTATC                       <  SRR4302542.3810480/94‑1 (MQ=60)
                                                                        GTGGGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTATC                       <  SRR4302542.7015053/94‑1 (MQ=60)
                                                                        GTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTATC                       <  SRR4302542.66326/94‑1 (MQ=60)
                                                                          GGGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTATCAT                     <  SRR4302542.3355134/94‑1 (MQ=60)
                                                                           GGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTATCATC                    <  SRR4302542.3107889/94‑1 (MQ=60)
                                                                            GCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTATCATCG                   <  SRR4302542.270056/94‑1 (MQ=60)
                                                                               GTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTATCATCGTCT                >  SRR4302542.5627840/1‑94 (MQ=60)
                                                                                 TCAGTTCCGCCGTCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTATCATCGTCTTT              <  SRR4302542.1739380/94‑1 (MQ=60)
                                                                                   AGTTCCGCCGTCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGGTTATTTTTATCGTCTTTAA            >  SRR4302542.2632649/1‑94 (MQ=60)
                                                                                   AGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTATCATCGTCTTTAA            <  SRR4302542.4195692/94‑1 (MQ=60)
                                                                                    GTTCCGCCGTCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTATCATCGTCTTTAAC           <  SRR4302542.5941290/94‑1 (MQ=60)
                                                                                    GTTCCGCCGTCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTATCATCGTCTTTAAC           <  SRR4302542.229353/94‑1 (MQ=60)
                                                                                    GTTCCGCCGTCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTATCATCGTCTTTAAC           <  SRR4302542.4119661/94‑1 (MQ=60)
                                                                                    GTTCCGCCGTCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTATCATCGTCTTTAAC           <  SRR4302542.2587336/94‑1 (MQ=60)
                                                                                    GTTCCGCCGTCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTATCATCGTCTTTAAC           <  SRR4302542.5665500/94‑1 (MQ=60)
                                                                                        CGCCGTCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTATCATCGTCTTTAAATGCC       >  SRR4302542.3537919/1‑94 (MQ=60)
                                                                                          CCGTCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTATCATCGTCTTTAACTGCCAG     <  SRR4302542.7506440/94‑1 (MQ=60)
                                                                                           CGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTATCATCGTCTTTAACTGCCAGG    <  SRR4302542.1929385/94‑1 (MQ=60)
                                                                                            GTCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTTTCATTGTCTTTAAATGCCCGGC   >  SRR4302542.4218023/1‑94 (MQ=60)
                                                                                             TCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTATCATCGTCTTTAACTGCCAGGCG  <  SRR4302542.3712132/94‑1 (MQ=60)
                                                                                             |                                                                                             
GTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTATCATCGTCTTTAACTGCCAGGCG  >  NC_000913/3250072‑3250258

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑