Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1061 294.1 15065038 98.6% 14854127 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,253,742 Δ1 bp coding (551/552 nt) yhaNM ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,253,7410G.99.6% 1160.7 / ‑5.8 257coding (552/552 nt)yhaNMhypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base . (135/120);  total (136/121)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 272 total aligned reads displayed.

TAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGA  >  NC_000913/3253650‑3253829
                                                                                           |                                                                                        
tAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCgt                                                                                         >  2:287165/1‑92 (MQ=255)
tAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCgt                                                                                         <  2:7181204/93‑2 (MQ=255)
tAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCgt                                                                                         >  1:4230767/1‑92 (MQ=255)
tAACGCACAATTTAGCTTCGCGGAAGCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCgt                                                                                         <  1:2314012/93‑2 (MQ=255)
  aCGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCgttc                                                                                       >  2:2120113/1‑90 (MQ=255)
   cGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCgttct                                                                                      <  1:5544505/93‑5 (MQ=255)
      acaATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGa                                                                                  >  2:4931009/1‑93 (MQ=255)
      acaATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGa                                                                                  >  1:4601307/1‑93 (MQ=255)
      acaATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGa                                                                                  >  2:6331059/1‑93 (MQ=255)
      acaATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGa                                                                                  >  2:6209749/1‑93 (MQ=255)
        aaTTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGaaa                                                                                >  1:7028804/1‑93 (MQ=255)
        aaTTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGaaa                                                                                <  2:3519841/93‑1 (MQ=255)
          tttAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGc                                                                              >  1:6672604/1‑93 (MQ=255)
            tAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCAc                                                                            <  2:773031/93‑1 (MQ=255)
            tAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCAc                                                                            <  2:1983383/93‑1 (MQ=255)
             aGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCAcc                                                                           <  1:4939328/93‑1 (MQ=255)
               cTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGt                                                                         <  1:1874629/93‑1 (MQ=255)
               cTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGAAAAGCACCGt                                                                         <  1:6424605/93‑1 (MQ=255)
                ttCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGACCTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTc                                                                        <  1:3659740/93‑1 (MQ=255)
                ttCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTc                                                                        <  2:6846428/93‑1 (MQ=255)
                ttCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTc                                                                        >  1:1310801/1‑93 (MQ=255)
                 tCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCa                                                                       <  2:3335919/93‑1 (MQ=255)
                 tCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCa                                                                       <  2:6785508/93‑1 (MQ=255)
                 tCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCa                                                                       <  1:1703703/93‑1 (MQ=255)
                  cgcgGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAg                                                                      <  2:3935271/93‑1 (MQ=255)
                  cgcgGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAg                                                                      <  1:957626/93‑1 (MQ=255)
                      gAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGga                                                                  >  2:1088370/1‑93 (MQ=255)
                      gAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGga                                                                  >  2:2657269/1‑93 (MQ=255)
                       aaTCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGgag                                                                 >  1:2135135/1‑93 (MQ=255)
                        aTCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGgaga                                                                <  2:7297453/93‑1 (MQ=255)
                        aTCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGgaga                                                                >  1:994682/1‑93 (MQ=255)
                        aTCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGgaga                                                                >  2:5955516/1‑93 (MQ=255)
                          cGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGAct                                                              <  2:1271570/93‑1 (MQ=255)
                           gagaATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGActc                                                             <  1:6511706/93‑1 (MQ=255)
                           gagaATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGActc                                                             <  1:891519/93‑1 (MQ=255)
                             gaATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCtt                                                           >  2:2216428/1‑93 (MQ=255)
                             gaATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCtt                                                           >  1:4464266/1‑93 (MQ=255)
                               aTAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGc                                                         >  1:3356005/1‑93 (MQ=255)
                                tAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCt                                                        >  2:3742372/1‑93 (MQ=255)
                                 aaaGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTc                                                       >  2:7403319/1‑93 (MQ=255)
                                   aGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGc                                                     >  1:3018528/1‑93 (MQ=255)
                                     cGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGccc                                                   <  2:6684739/93‑1 (MQ=255)
                                      ggATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCct                                                  >  2:1156354/1‑93 (MQ=255)
                                       gATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCctc                                                 >  2:4756265/1‑93 (MQ=255)
                                       gATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCctc                                                 >  1:5991626/1‑93 (MQ=255)
                                        aTCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCctct                                                <  2:2965665/93‑1 (MQ=255)
                                         tCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTg                                               >  1:2679685/1‑93 (MQ=255)
                                          cgccgcAAACGGGACTTCATTGACGAACTTCAGGATGTCAGCCAGCGT‑AGTGCTGGAAAGCAGCGTCAGCGGAGTCTCTTGCTCGCCCTCTGc                                              <  1:7338451/93‑1 (MQ=255)
                                            ccgcAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCca                                            <  1:4833246/93‑1 (MQ=255)
                                             cgcAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCcac                                           >  2:5744561/1‑93 (MQ=255)
                                              gcAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCcaca                                          >  1:1054424/1‑93 (MQ=255)
                                              gcAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCcaca                                          >  2:5353540/1‑93 (MQ=255)
                                               cAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCcacac                                         >  2:3400510/1‑93 (MQ=255)
                                                aaaCGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCcacaca                                        <  2:1835345/93‑1 (MQ=255)
                                                aaaCGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCcacaca                                        <  1:5110353/93‑1 (MQ=255)
                                                aaaCGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCcacaca                                        >  2:5049298/1‑93 (MQ=255)
                                                 aaCGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCacacac                                       >  2:438726/1‑93 (MQ=255)
                                                 aaCGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCacacac                                       >  2:6379109/1‑93 (MQ=255)
                                                 aaCGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCacacac                                       >  1:6224328/1‑93 (MQ=255)
                                                 aaCGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCacacac                                       >  1:4133625/1‑93 (MQ=255)
                                                   cGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGc                                     <  2:4498756/93‑1 (MQ=255)
                                                   cGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGc                                     <  1:611204/93‑1 (MQ=255)
                                                    gggACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGcc                                    <  1:6800225/93‑1 (MQ=255)
                                                    gggACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGcc                                    <  1:2875394/93‑1 (MQ=255)
                                                    gggACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGcc                                    <  2:5446946/93‑1 (MQ=255)
                                                    gggACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGcc                                    <  1:3984385/93‑1 (MQ=255)
                                                     ggACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCct                                   >  2:2361763/1‑93 (MQ=255)
                                                     ggACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCct                                   <  2:3786018/93‑1 (MQ=255)
                                                      gACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCctg                                  >  1:1978663/1‑93 (MQ=255)
                                                      gACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCctg                                  >  1:7435920/1‑93 (MQ=255)
                                                       aCTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCctgc                                 >  2:6101697/1‑93 (MQ=255)
                                                         ttCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCctgctg                               >  2:3030199/1‑93 (MQ=255)
                                                          tCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTgg                              >  2:5862596/1‑93 (MQ=255)
                                                           cATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTggg                             <  2:5606788/93‑1 (MQ=255)
                                                           cATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTggg                             <  1:1943544/93‑1 (MQ=255)
                                                            aTTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGt                            >  2:3605491/1‑93 (MQ=255)
                                                             ttGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTa                           >  2:4659369/1‑93 (MQ=255)
                                                             ttGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTa                           >  1:7324579/1‑93 (MQ=255)
                                                                aCGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAac                        <  2:7048098/93‑1 (MQ=255)
                                                                  gAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAacac                      <  1:120436/93‑1 (MQ=255)
                                                                   aaCTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAacacc                     <  1:5626244/93‑1 (MQ=255)
                                                                   aaCTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAacacc                     <  2:1344956/93‑1 (MQ=255)
                                                                    aCTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAacacca                    >  1:7188993/1‑93 (MQ=255)
                                                                     cTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAacaccac                   >  2:4216358/1‑93 (MQ=255)
                                                                     cTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAacaccac                   >  1:1041254/1‑93 (MQ=255)
                                                                      ttCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAacaccaca                  >  2:3435779/1‑93 (MQ=255)
                                                                      ttCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAacaccaca                  >  2:2470473/1‑93 (MQ=255)
                                                                        cAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAacaccacacc                <  2:921427/93‑1 (MQ=255)
                                                                         aGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAAcaccacacca               >  1:1476652/1‑93 (MQ=255)
                                                                         aGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAAcaccacacca               <  2:147880/93‑1 (MQ=255)
                                                                           gATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATc             >  1:7508999/1‑93 (MQ=255)
                                                                           gATCTCAGCCAGCGTC‑GTT‑TGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCg            >  1:2983273/1‑93 (MQ=255)
                                                                            aTCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCg            >  1:3113727/1‑93 (MQ=255)
                                                                             tctcAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGt           >  1:7056797/1‑93 (MQ=255)
                                                                              ctcAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTg          <  2:2441240/93‑1 (MQ=255)
                                                                                cagccagcGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCg        >  1:2588814/1‑93 (MQ=255)
                                                                                  gccagcGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGtc      >  1:605774/1‑93 (MQ=255)
                                                                                   ccagcGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGtct     >  2:1685720/1‑93 (MQ=255)
                                                                                     agcGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCg   >  1:3571352/1‑93 (MQ=255)
                                                                                      gcGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGa  <  1:5513978/93‑1 (MQ=255)
                                                                                           |                                                                                        
TAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGA  >  NC_000913/3253650‑3253829

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ATAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGC  >  NC_000913/3253649‑3253834
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ATAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGT                                                                                              >  SRR4302542.4272440/1‑94 (MQ=60)
 TAACGCACAATTTAGCTTCGCGGAAGCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTT                                                                                             <  SRR4302542.2333873/94‑1 (MQ=60)
   ACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCT                                                                                          <  SRR4302542.3686016/94‑1 (MQ=60)
   ACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCT                                                                                          <  SRR4302542.7242805/94‑1 (MQ=60)
    CGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTG                                                                                         >  SRR4302542.4913270/1‑94 (MQ=60)
    CGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTG                                                                                         <  SRR4302542.5598389/94‑1 (MQ=60)
      CACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGA                                                                                       >  SRR4302542.7565310/1‑94 (MQ=60)
      CACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGA                                                                                       >  SRR4302542.4646282/1‑94 (MQ=60)
        CAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGGAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCCGCGGCGGTTC‑GGAAA                                                                                     >  SRR4302542.6268383/1‑94 (MQ=60)
        CAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAA                                                                                     >  SRR4302542.7097871/1‑94 (MQ=60)
        CAATGTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAA                                                                                     >  SRR4302542.7035212/1‑94 (MQ=60)
          ATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGC                                                                                   >  SRR4302542.4115269/1‑94 (MQ=60)
          ATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGC                                                                                   >  SRR4302542.6738339/1‑94 (MQ=60)
          ATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGC                                                                                   <  SRR4302542.1076733/94‑1 (MQ=60)
           TTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCA                                                                                  >  SRR4302542.5864306/1‑94 (MQ=60)
           TTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCA                                                                                  <  SRR4302542.6656043/94‑1 (MQ=60)
            TTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCAC                                                                                 <  SRR4302542.5430700/94‑1 (MQ=60)
             TAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACC                                                                                <  SRR4302542.4663181/94‑1 (MQ=60)
             TAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACC                                                                                <  SRR4302542.4262961/94‑1 (MQ=60)
             TAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACC                                                                                <  SRR4302542.5077734/94‑1 (MQ=60)
             TAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACC                                                                                <  SRR4302542.4476147/94‑1 (MQ=60)
              AGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCG                                                                               <  SRR4302542.4987332/94‑1 (MQ=60)
              AGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCG                                                                               >  SRR4302542.5660594/1‑94 (MQ=60)
               GCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGT                                                                              <  SRR4302542.151412/94‑1 (MQ=60)
               GCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGT                                                                              >  SRR4302542.1085819/1‑94 (MQ=60)
                CTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTC                                                                             <  SRR4302542.1890845/94‑1 (MQ=60)
                CTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTC                                                                             >  SRR4302542.6747060/1‑94 (MQ=60)
                CTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTC                                                                             >  SRR4302542.1322098/1‑94 (MQ=60)
                CTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGAAAAGCACCGTC                                                                             <  SRR4302542.6487914/94‑1 (MQ=60)
                 TTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGACCTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCA                                                                            <  SRR4302542.3694208/94‑1 (MQ=60)
                  TCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAG                                                                           <  SRR4302542.1718469/94‑1 (MQ=60)
                  TCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAG                                                                           <  SRR4302542.7102722/94‑1 (MQ=60)
                   CGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGC                                                                          <  SRR4302542.965851/94‑1 (MQ=60)
                     CGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGG                                                                        <  SRR4302542.2876265/94‑1 (MQ=60)
                     CGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGG                                                                        <  SRR4302542.3117851/94‑1 (MQ=60)
                       GAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAG                                                                      >  SRR4302542.2285083/1‑94 (MQ=60)
                       GAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAG                                                                      >  SRR4302542.2153564/1‑94 (MQ=60)
                       GAATCGAGAATAAAGCGGATCGCCGCAAACGGGACCTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTTAGCGGAG                                                                      >  SRR4302542.6055699/1‑94 (MQ=60)
                        AATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGA                                                                     >  SRR4302542.172296/1‑94 (MQ=60)
                        AATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGA                                                                     >  SRR4302542.1003242/1‑94 (MQ=60)
                         ATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGAC                                                                    >  SRR4302542.1309436/1‑94 (MQ=60)
                            GAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCT                                                                 <  SRR4302542.6575887/94‑1 (MQ=60)
                            GAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCT                                                                 >  SRR4302542.6875443/1‑94 (MQ=60)
                            GAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCT                                                                 >  SRR4302542.4009303/1‑94 (MQ=60)
                            GAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCT                                                                 <  SRR4302542.899242/94‑1 (MQ=60)
                             AGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTT                                                                <  SRR4302542.4396918/94‑1 (MQ=60)
                             AGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTT                                                                <  SRR4302542.6675395/94‑1 (MQ=60)
                             AGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTT                                                                >  SRR4302542.4508055/1‑94 (MQ=60)
                             AGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTT                                                                >  SRR4302542.3103954/1‑94 (MQ=60)
                               AATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGC                                                              <  SRR4302542.2348948/94‑1 (MQ=60)
                               AATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGC                                                              >  SRR4302542.3387565/1‑94 (MQ=60)
                                ATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCT                                                             <  SRR4302542.7232643/94‑1 (MQ=60)
                                ATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCT                                                             <  SRR4302542.5594736/94‑1 (MQ=60)
                                ATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCT                                                             <  SRR4302542.888305/94‑1 (MQ=60)
                                   AAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGC                                                          >  SRR4302542.3047364/1‑94 (MQ=60)
                                    AGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCC                                                         >  SRR4302542.1463978/1‑94 (MQ=60)
                                      CGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCT                                                       >  SRR4302542.2117660/1‑94 (MQ=60)
                                       GGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTC                                                      >  SRR4302542.4836306/1‑94 (MQ=60)
                                       GGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTC                                                      >  SRR4302542.6049661/1‑94 (MQ=60)
                                        GATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCT                                                     <  SRR4302542.2744801/94‑1 (MQ=60)
                                        GATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCT                                                     <  SRR4302542.7398517/94‑1 (MQ=60)
                                         ATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTG                                                    <  SRR4302542.901168/94‑1 (MQ=60)
                                         ATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTG                                                    >  SRR4302542.2705547/1‑94 (MQ=60)
                                          TCGCCGCAAACGGGACTTCATTGACGAAGTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGC                                                   >  SRR4302542.5716731/1‑94 (MQ=60)
                                          TCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGC                                                   <  SRR4302542.3157379/94‑1 (MQ=60)
                                           CGCCGCAAACGGGACTTCATTGACGAACTTCAGGATGTCAGCCAGCGT‑AGTGCTGGAAAGCAGCGTCAGCGGAGTCTCTTGCTCGCCCTCTGCC                                                  <  SRR4302542.7410334/94‑1 (MQ=60)
                                            GCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCA                                                 <  SRR4302542.2274006/94‑1 (MQ=60)
                                             CCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCAC                                                <  SRR4302542.4880332/94‑1 (MQ=60)
                                              CGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACA                                               >  SRR4302542.1063489/1‑94 (MQ=60)
                                              CGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACA                                               >  SRR4302542.411301/1‑94 (MQ=60)
                                              CGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACA                                               >  SRR4302542.1063278/1‑94 (MQ=60)
                                                 AAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACAC                                            >  SRR4302542.6285624/1‑94 (MQ=60)
                                                 AAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACAC                                            <  SRR4302542.5159990/94‑1 (MQ=60)
                                                 AAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACAC                                            >  SRR4302542.4174358/1‑94 (MQ=60)
                                                  AACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACG                                           >  SRR4302542.6153137/1‑94 (MQ=60)
                                                   ACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGC                                          <  SRR4302542.4562239/94‑1 (MQ=60)
                                                    CGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCC                                         <  SRR4302542.4565651/94‑1 (MQ=60)
                                                    CGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCC                                         <  SRR4302542.616461/94‑1 (MQ=60)
                                                    CGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCC                                         >  SRR4302542.5089906/1‑94 (MQ=60)
                                                     GGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCT                                        >  SRR4302542.1985505/1‑94 (MQ=60)
                                                     GGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCT                                        <  SRR4302542.6867088/94‑1 (MQ=60)
                                                     GGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCT                                        <  SRR4302542.4023694/94‑1 (MQ=60)
                                                     GGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCT                                        <  SRR4302542.2903003/94‑1 (MQ=60)
                                                      GGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTG                                       >  SRR4302542.7508697/1‑94 (MQ=60)
                                                      GGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTG                                       >  SRR4302542.1995751/1‑94 (MQ=60)
                                                        ACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCT                                     <  SRR4302542.6336994/94‑1 (MQ=60)
                                                         CTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTG                                    <  SRR4302542.124938/94‑1 (MQ=60)
                                                         CTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTG                                    <  SRR4302542.7391947/94‑1 (MQ=60)
                                                         CTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTG                                    <  SRR4302542.6518540/94‑1 (MQ=60)
                                                         CTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTG                                    >  SRR4302542.6343381/1‑94 (MQ=60)
                                                         CTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGACAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTG                                    >  SRR4302542.7442712/1‑94 (MQ=60)
                                                          TTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGG                                   <  SRR4302542.3594388/94‑1 (MQ=60)
                                                            CATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGT                                 <  SRR4302542.5545025/94‑1 (MQ=60)
                                                            CATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGT                                 <  SRR4302542.1960351/94‑1 (MQ=60)
                                                             ATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTA                                >  SRR4302542.7396322/1‑94 (MQ=60)
                                                               TGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCATCTGCCACACACGCCTGCTGGGTAAA                              >  SRR4302542.7502407/1‑94 (MQ=60)
                                                                GACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAAC                             <  SRR4302542.5622043/94‑1 (MQ=60)
                                                                GACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAAC                             <  SRR4302542.1951337/94‑1 (MQ=60)
                                                                 ACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACA                            >  SRR4302542.3789562/1‑94 (MQ=60)
                                                                 ACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACA                            <  SRR4302542.6277138/94‑1 (MQ=60)
                                                                  CGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACAC                           >  SRR4302542.1935868/1‑94 (MQ=60)
                                                                   GAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACC                          <  SRR4302542.121430/94‑1 (MQ=60)
                                                                   GAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACC                          >  SRR4302542.2065916/1‑94 (MQ=60)
                                                                    AACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCCCCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCA                         <  SRR4302542.7598556/94‑1 (MQ=60)
                                                                    AACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCA                         <  SRR4302542.5680876/94‑1 (MQ=60)
                                                                    AACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCA                         >  SRR4302542.7259534/1‑94 (MQ=60)
                                                                     ACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCAC                        >  SRR4302542.1050193/1‑94 (MQ=60)
                                                                     ACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCAC                        >  SRR4302542.2623993/1‑94 (MQ=60)
                                                                     AATTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCAC                        >  SRR4302542.269257/1‑94 (MQ=60)
                                                                       TTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACAC                      >  SRR4302542.1002552/1‑94 (MQ=60)
                                                                       TTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACAC                      >  SRR4302542.2966534/1‑94 (MQ=60)
                                                                         CAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCA                    >  SRR4302542.1489390/1‑94 (MQ=60)
                                                                         CAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCA                    >  SRR4302542.331894/1‑94 (MQ=60)
                                                                           GGATCTCAGCCAGCGTCG‑‑TTTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCG                 >  SRR4302542.3011836/1‑94 (MQ=60)
                                                                           GGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATC                  >  SRR4302542.7582852/1‑94 (MQ=60)
                                                                           GGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATC                  >  SRR4302542.3610954/1‑94 (MQ=60)
                                                                           GGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACCCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATC                  <  SRR4302542.6776450/94‑1 (MQ=60)
                                                                            GATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCG                 <  SRR4302542.7521134/94‑1 (MQ=60)
                                                                            GATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCG                 >  SRR4302542.3143312/1‑94 (MQ=60)
                                                                             ATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGT                <  SRR4302542.1552646/94‑1 (MQ=60)
                                                                             ATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGT                >  SRR4302542.7126065/1‑94 (MQ=60)
                                                                              TCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCaagagtctccgctgacagtgctt               <  SRR4302542.1720088/94‑24 (MQ=60)
                                                                              TCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTG               <  SRR4302542.966797/94‑1 (MQ=60)
                                                                              TCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTG               <  SRR4302542.1809388/94‑1 (MQ=60)
                                                                                TCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCG             >  SRR4302542.2613891/1‑94 (MQ=60)
                                                                                  AGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTC           >  SRR4302542.610989/1‑94 (MQ=60)
                                                                                  AGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTC           >  SRR4302542.6057862/1‑94 (MQ=60)
                                                                                    CCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTC         >  SRR4302542.2981980/1‑94 (MQ=60)
                                                                                     CAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCG        >  SRR4302542.3604685/1‑94 (MQ=60)
                                                                                     CAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCG        <  SRR4302542.7304262/94‑1 (MQ=60)
                                                                                       GCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGAT      <  SRR4302542.5567564/94‑1 (MQ=60)
                                                                                        CGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATA     >  SRR4302542.5188535/1‑94 (MQ=60)
                                                                                        CGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATA     <  SRR4302542.6543251/94‑1 (MQ=60)
                                                                                        CGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATA     >  SRR4302542.428024/1‑94 (MQ=60)
                                                                                        CGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATA     <  SRR4302542.4010958/94‑1 (MQ=60)
                                                                                        CGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATA     >  SRR4302542.6555978/1‑94 (MQ=60)
                                                                                        CGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATA     >  SRR4302542.3953791/1‑94 (MQ=60)
                                                                                        CGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATA     >  SRR4302542.1490012/1‑94 (MQ=60)
                                                                                         GTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATAT    >  SRR4302542.741424/1‑94 (MQ=60)
                                                                                         GTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATAT    >  SRR4302542.6998581/1‑94 (MQ=60)
                                                                                         GTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATAT    >  SRR4302542.2275536/1‑94 (MQ=60)
                                                                                         GTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATAT    <  SRR4302542.1670676/94‑1 (MQ=60)
                                                                                         GTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACAAGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATAT    >  SRR4302542.7539161/1‑94 (MQ=60)
                                                                                           tcGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATG   <  SRR4302542.4502485/92‑1 (MQ=60)
                                                                                            CGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGC  >  SRR4302542.2634983/1‑94 (MQ=60)
                                                                                             |                                                                                            
ATAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGC  >  NC_000913/3253649‑3253834

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑