Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1061 294.1 15065038 98.6% 14854127 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,304,497 A→G R352R (CGT→CGC)  deaD ← inducible ATP‑independent RNA helicase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,304,4970AG87.2% 709.5 / 87.2 296R352R (CGT→CGC) deaDinducible ATP‑independent RNA helicase
Reads supporting (aligned to +/- strand):  ref base A (15/23);  new base G (116/142);  total (131/165)
Fisher's exact test for biased strand distribution p-value = 6.01e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.19e-01

Only 100 of 296 total aligned reads displayed.

GCTTCATAGTACGTTCAATGTTGCGCAGCAGACGACGCTCGCGGTTCTCAACGAACAGCAGCGCGCGGCCAGCACGACCCGCACGACCGGTACGACCGATACGGTGAACGTAAGACTCAGAATCCATCGGGATATCGTAGTTAACTACCAGGCTGATACGCTCAACGTCCAGGCCACGGGC  >  NC_000913/3304406‑3304586
                                                                                           |                                                                                         
gCTTCATAGTACGTTCAATGTTGCGCAGCAGACGACGCTCGCGGTTCTCAACGAACAGCAGCGCGCGGCCAGCACGACCCGCACGACCGGTAc                                                                                          <  1:3272485/93‑1 (MQ=255)
   tCATAGTACGTTCAATGTTGCGCAGCAGACGACGCTCGCGGTTCTCAACGAACAGCAGCGCGCGGCCAGCACGACCCGCACGACCGGTACGAc                                                                                       <  1:1477392/93‑1 (MQ=255)
   tCATAGTACGTTCAATGTTGCGCAGCAGACGACGCTCGCGGTTCTCAACGAACAGCAGCGCGCGGCCAGCACGACCCGCACGACCGGTACGAc                                                                                       <  2:7391773/93‑1 (MQ=255)
    cATAGTACGTTCAATGTTGCGCAGCAGACGACGATCGCGGTTCTCAACGAACAGCAGCGCGCGGCCAGCACGACCCGCACGACCGGTGCGAcc                                                                                      >  1:2394752/1‑93 (MQ=255)
     aTAGTACGTTCAATGTTGCGCAGCAGACGACGCTCGCGGTTCTCAACGAACAGCAGCGCGCGGCCAGCACGACCCGCACGACCGGTGCGACCg                                                                                     >  2:457117/1‑93 (MQ=255)
      tAGTACGTTCAATGTTGCGCAGCAGACGACGCTCGCGGTTCTCAACGAACAGCAGCGCGCGGCCAGCACGACCCGCACGACCGGTGCGACCGa                                                                                    <  2:7230478/93‑1 (MQ=255)
      tAGTACGTTCAATGTTGCGCAGCAGACGACGCTCGCGGTTCTCAACGAACAGCAGCGCGCGGCCAGCACGACCCGCACGACCGGTGCGACCGa                                                                                    >  2:3394772/1‑93 (MQ=255)
        gTACGTTCAATGTTGCGCAGCAGACGACGCTCGCGGTTCTCAACGAACAGCAGCGCGCGGCCAGCACGACCCGCACGACCGGTGCGACCGATa                                                                                  >  1:2773134/1‑93 (MQ=255)
         tACGTTCAATGTTGCGCAGCAGACGACGCTCGCGGTTCTCAACGAACAGCAGCGCGCGGCCAGCACGACCCGCACGACCGGTGCGACCGATAc                                                                                 <  1:4021875/93‑1 (MQ=255)
         tACGTTCAATGTTGCGCAGCAGACGACGCTCGCGGTTCTCAACGAACAGCAGCGCGCGGCCAGCACGACCCGCACGACCGGTGCGACCGATAc                                                                                 <  2:2608688/93‑1 (MQ=255)
         tACGTTCAATGTTGCGCAGCAGACGACGCTCGCGGTTCTCAACGAACAGCAGCGCGCGGCCAGCACGACCCGCACGACCGGTGCGACCGATAc                                                                                 <  2:3064399/93‑1 (MQ=255)
         tACGTTCAATGTTGCGCAGCAGACGACGCTCGCGGTTCTCAACGAACAGCAGCGCGCGGCCAGCACGACCCGCACGACCGGTGCGACCGATAc                                                                                 >  1:876000/1‑93 (MQ=255)
         tACGTTCAATGTTGCGCAGCAGACGACGCTCGCGGTTCTCAACGAACAGCAGCGCGCGGCCAGCACGACCCGCACGACCGGTGCGACCGATAc                                                                                 <  1:7138266/93‑1 (MQ=255)
          aCGTTCAATGTTGCGCAGCAGACGACGCTCGCGGTTCTCAACGAACAGCAGCGCGCGGCCAGCACGACCCGCACGACCGGTGCGACCGATACg                                                                                <  2:3833795/93‑1 (MQ=255)
          aCGTTCAATGTTGCGCAGCAGACGACGCTCGCGGTTCTCAACGAACAGCAGCGCGCGGCCAGCACGACCCGCACGACCGGTGCGACCGATACg                                                                                >  1:6897801/1‑93 (MQ=255)
           cGTTCAATGTTGCGCAGCAGACGACGCTCGCGGTTCTCAACGAACAGCAGCGCGCGGCCAGCACGACCCGCACGACCGGTGCGACCGATACgg                                                                               <  2:3772145/93‑1 (MQ=255)
           cGTTCAATGTTGCGCAGCAGACGACGCTCGCGGTTCTCAACGAACAGCAGCGCGCGGCCAGCACGACCCGCACGACCGGTGCGACCGATACgg                                                                               <  2:487898/93‑1 (MQ=255)
           cGTTCAATGTTGCGCAGCAGACGACGCTCGCGGTTCTCAACGAACAGCAGCGCGCGGCCAGCACGACCCGCACGACCGGTGCGACCGATACgg                                                                               >  1:3944560/1‑93 (MQ=255)
            gTTCAATGTTGCGCAGCAGACGACGCTCGCGGTTCTCAACGAACAGCAGCGCGCGGCCAGCACGACCCGCACGACCGGTACGACCGATACGGt                                                                              <  1:3599961/93‑1 (MQ=255)
               cAATGTTGCGCAGCAGACGACGCTCGCGGTTCTCAACGAACAGCAGCGCGCGGCCAGCACGACCCGCACGACCGGTGCGACCGATACGGTGaa                                                                           <  2:1239862/93‑1 (MQ=255)
                aaTGTTGCGCAGCAGACGACGCTCGCGGTTCTCAACGAACAGCAGCGCGCGGCCAGCACGACCCGCACGACCGGTGCGACCGATACGGTGAAc                                                                          <  2:1048161/93‑1 (MQ=255)
                aaTGTTGCGCAGCAGACGACGCTCGCGGTTCTCAACGAACAGCAGCGCGCGGCCAGCACGACCCGCACGACCGGTGCGACCGATACGGTGAAc                                                                          <  1:2189835/93‑1 (MQ=255)
                    ttGCGCAGCAGACGACGCTCGCGGTTCTCAACGAACAGCAGCGCGCGGCCAGCACGACCCGCACGACCGGTGCGACCGATACGGTGAACGTaa                                                                      <  2:6072456/93‑1 (MQ=255)
                        gcagcaGACGACGCTCGCGGTTCTCAACGAACAGCAGCGCGCGGCCAGCACGACCCGCACGACCGGTGCGACCGATACGGTGAACGTAAGACt                                                                  >  1:2436758/1‑93 (MQ=255)
                         cagcagACGACGCTCGCGGTTCTCAACGAACAGCAGCGCGCGGCCAGCACGACCCGCACGACCGGTGCGACCGATACGGTGAACGTAAGACTc                                                                 >  1:4962764/1‑93 (MQ=255)
                            cagACGACGCTCGCGGTTCTCAACGAACAGCAGCGCGCGGCCAGCACGACCCGCACGACCGGTGCGACCGATACGGTGAACGTAAGACTCAGa                                                              <  1:1015632/93‑1 (MQ=255)
                             agACGACGCTCGCGGTTCTCAACGAACAGCAGCGCGCGGCCAGCACGACCCGCACGACCGGTGCGACCGATACGGTGAACGTAAGACTCAGaa                                                             <  1:7245435/93‑1 (MQ=255)
                             agACGACGCTCGCGGTTCTCAACGAACAGCAGCGCGCGGCCAGCACGACCCGCACGACCGGTGCGACCGATACGGTGAACGTAAGACTCAGaa                                                             <  2:1196378/93‑1 (MQ=255)
                              gacgacGCTCGCGGTTCTCAACGAACAGCAGCGCGCGGCCAGCACGACCCGCACGACCGGTGCGACCGATACGGTGAACGTAAGACTCAGAAt                                                            >  1:4075498/1‑93 (MQ=255)
                              gacgacGCTCGCGGTTCTCAACGAACAGCAGCGCGCGGCCAGCACGACCCGCACGACCGGTGCGACCGATACGGTGAACGTAAGACTCAGAAt                                                            <  1:484236/93‑1 (MQ=255)
                              gacgacGCTCGCGGTTCTCAACGAACAGCAGCGCGCGGCCAGCACGACCCGCACGACCGGTGCGACCGATACGGTGAACGTAAGACTCAGAAt                                                            >  2:2126689/1‑93 (MQ=255)
                              gacgacGCTCGCGGTTCTCAACGAACAGCAGCGCGCGGCCAGCACGACCCGCACGACCGGTGCGACCGATACGGTGAACCTAAGACTCAGAAt                                                            >  2:4532304/1‑93 (MQ=255)
                               acgacgCTCGCGGTTCTCAACGAACAGCAGCGCGCGGCCAGCACGACCCGCACGACCGGTGCGACCGATACGGTGAACGTAAGACTCAGAATc                                                           <  2:3594041/93‑1 (MQ=255)
                               acgacgCTCGCGGTTCTCAACGAACAGCAGCGCGCGGCCAGCACGACCCGCACGACCGGTGCGACCGATACGGTGAACGTAAGACTCAGAATc                                                           <  1:302705/93‑1 (MQ=255)
                                  acgCTCGCGGTTCTCAACGAACAGCAGCGCGCGGCCAGCACGACCCGCACGACCGGTGCGACCGATACGGTGAACGTAAGACTCAGAATCCAt                                                        >  2:4820129/1‑93 (MQ=255)
                                   cgCTCGCGGTTCTCAACGAACAGCAGCGCGCGGCCAGCACGACCCGCACGACCGGTGCGACCGATACGGTGAACGTAAGACTCAGAATCCATc                                                       <  2:667864/93‑1 (MQ=255)
                                   cgCTCGCGGTTCTCAACGAACAGCAGCGCGCGGCCAGCACGACCCGCACGACCGGTGCGACCGATACGGTGAACGTAAGACTCAGAATCCATc                                                       <  2:7034353/93‑1 (MQ=255)
                                   cgCTCGCGGTTCTCAACGAACAGCAGCGCGCGGCCAGCACGACCCGCACGACCGGTACGACCGATACGGTGAACGTAAGACTCAGAATCCATc                                                       >  2:5627665/1‑93 (MQ=255)
                                    gCTCGCGGTTCTCAACGAACAGCAGCGCGCGGCCAGCACGACCCGCACGACCGGTGCGACCGATACGGTGAACGTAAGACTCAGAATCCATCg                                                      <  1:4134404/93‑1 (MQ=255)
                                    gCTCGCGGTTCTCAACGAACAGCAGCGCGCGGCCAGCACGACCCGCACGACCGGTGCGACCGATACGGTGAACGTAAGACTCAGAATCCATCg                                                      >  2:6632724/1‑93 (MQ=255)
                                     cTCGCGGTTCTCAACGAACAGCAGCGCGCGGCCAGCACGACCCGCACGACCGGTGCGACCGATACGGTGAACGTAAGACTCAGAATCCATCgg                                                     <  2:2938284/93‑1 (MQ=255)
                                     cTCGCGGTTCTCAACGAACAGCAGCGCGCGGCCAGCACGACCCGCACGACCGGTGCGACCGATACGGTGAACGTAAGACTCAGAATCCATCgg                                                     <  2:6193888/93‑1 (MQ=255)
                                      tCGCGGTTCTCAACGAACAGCAGCGCGCGGCCAGCACGACCCGCACGACCGGTGCGACCGATACGGTGAACGTAAGACTCAGAATCCATCggg                                                    >  1:6541983/1‑93 (MQ=255)
                                       cgcgGTTCTCAACGAACAGCAGCGCGCGGCCAGCACGACCCGCACGACCGGTGCGACCGATAACGCGAACGTAAGACTCAGAATCCATCGGGa                                                   >  1:7461780/1‑93 (MQ=255)
                                         cgGTTCTCAACGAACAGCAGCGCGCGGCCAGCACGACCCGCACGACCGGTGCGACCGATACGGTGAACGTAAGACTCAGAATCCATCGGGata                                                 >  2:3455414/1‑93 (MQ=255)
                                         cgGTTCTCAACGAACAGCAGCGCGCGGCCAGCACGACCCGCACGACCGGTGCGACCGATACGGTGAACGTAAGACTCAGAATCCATCGGGata                                                 >  2:7510568/1‑93 (MQ=255)
                                          ggTTCTCAACGAACAGCAGCGCGCGGCCAGCACGACCCGCACGACCGGTGCGACCGATACGGTGAACGTAAGACTCAGAATCCATCGGGatat                                                >  2:6352866/1‑93 (MQ=255)
                                           gTTCTCAACGAACAGCAGCGCGCGGCCAGCACGACCCGCACGACCGGTGCGACCGATACGGTGAACGTAAGACTCAGAATCCATCGGGATATc                                               >  2:222013/1‑93 (MQ=255)
                                           gTTCTCAACGAACAGCAGCGCGCGGCCAGCACGACCCGCACGACCGGTGCGACCGATACGGTGAACGTAAGACTCAGAATCCATCGGGATATc                                               >  1:2031970/1‑93 (MQ=255)
                                           gTTCTCAACGAACAGCAGCGCGCGGCCAGCACGACCCGCACGACCGGTGCGACCGATACGGTGAACGTAAGACTCAGAATCCATCGGGATATc                                               >  1:6395181/1‑93 (MQ=255)
                                            ttCTCAACGAACAGCAGCGCGCGGCCAGCACGACCCGCACGACCGGTGCGACCGATACGGTGAACGTAAGACTCAGAATCCATCGGGATATCg                                              >  1:5592826/1‑93 (MQ=255)
                                            ttCTCAACGAACAGCAGCGCGCGGCCAGCACGACCCGCACGACCGGTGCGACCGATACGGTGAACGTAAGACTCAGAATCCATCGGGATATCg                                              <  1:4503578/93‑1 (MQ=255)
                                             tctcAACGAACAGCAGCGCGCGGCCAGCACGACCCGCACGACCGGTGCGACCGATACGGTGAACGTAAGACTCAGAATCCATCGGGATATCGt                                             >  1:2589077/1‑93 (MQ=255)
                                             tctcAACGAACAGCAGCGCGCGGCCAGCACGACCCGCACGACCGGTGCGACCGATACGGTGAACGTAAGACTCAGAATCCATCGGGATATCGt                                             <  2:1443781/93‑1 (MQ=255)
                                               tcAACGAACAGCAGCGCGCGGCCAGCACGACCCGCACGACCGGTGCGACCGATACGGTGAACGTAAGACTCAGAATCCATCGGGATATCGTAg                                           >  1:3497851/1‑93 (MQ=255)
                                               tcAACGAACAGCAGCGCGCGGCCAGCACGACCCGCACGACCGGTGCGACCGATACGGTGAACGTAAGACTCAGAATCCATCGGGATATCGTAg                                           >  1:3776002/1‑93 (MQ=255)
                                               tcAACGAACAGCAGCGCGCGGCCAGCACGACCCGCACGACCGGTGCGACCGATACGGTGAACGTAAGACTCAGAATCCATCGGGATATCGTAg                                           >  1:4282699/1‑93 (MQ=255)
                                               tcAACGAACAGCAGCGCGCGGCCAGCACGACCCGCACGACCGGTGCGACCGATACGGTGAACGTAAGACTCAGAATCCATCGGGATATCGTAg                                           >  2:3993833/1‑93 (MQ=255)
                                               tcAACGAACAGCAGCGCGCGGCCAGCACGACCCGCACGACCGGTGCGACCGATACGGTGAACGTAAGACTCAGAATCCATCGGGATATCGTAg                                           >  1:859520/1‑93 (MQ=255)
                                                 aaCGAACAGCAGCGCGCGGCCAGCACGACCCGCACGACCGGTACGACCGATACGGTGAACGTAAGACTCAGAATCCATCGGGATATCGTAGtt                                         >  1:1927593/1‑93 (MQ=255)
                                                   cGAACAGCAGCGCGCGGCCAGCACGACCCGCACGACCGGTGCGACCGATACGGTGAACGTAAGACTCAGAATCCATCGGGATATCGTAGTTaa                                       <  1:7025490/93‑1 (MQ=255)
                                                    gAACAGCAGCGCGCGGCCAGCACGACCCGCACGACCGGTGCGACCGATACGGTGAACGTAAGACTCAGAATCCATCGGGATATCGTAGTTAAc                                      <  1:118309/93‑1 (MQ=255)
                                                    gAACAGCAGCGCGCGGCCAGCACGACCCGCACGACCGGTGCGACCGATACGGTGAACGTAAGACTCAGAATCCATCGGGATATCGTAGTTAAc                                      <  2:3664930/93‑1 (MQ=255)
                                                    gAACAGCAGCGCGCGGCCAGCACGACCCGCACGACCGGTGCGACCGATACGGTGAACGTAAGACTCAGAATCCATCGGGATATCGTAGTTAAc                                      <  1:6714507/93‑1 (MQ=255)
                                                      aCAGCAGCGCGCGGCCAGCACGACCCGCACGACCGGTACGACCGATACGGTGAACGTAAGACTCAGAATCCATCGGGATATCGTAGTTAACTa                                    <  1:159818/93‑1 (MQ=255)
                                                         gcagcGCGCGGCCAGCACGACCCGCACGACCGGTGCGACCGATACGGTGAACGTAAGACTCAGAATCCATCGGGATATCGTAGTTAACTACCa                                 <  2:7316013/93‑1 (MQ=255)
                                                         gcagcGCGCGGCCAGCACGACCCGCACGACCGGTGCGACCGATACGGTGAACGTAAGACTCAGAATCCATCGGGATATCGTAGTTAACTACCa                                 >  2:2280378/1‑93 (MQ=255)
                                                         gcagcGCGCGGCCAGCACGACCCGCACGACCGGTGCGACCGATACGGTGAACGTAAGACTCAGAATCCATCGGGATATCGTAGTTAACTACCa                                 >  1:397358/1‑93 (MQ=255)
                                                         gcagcGCGCGGCCAGCACGACCCGCACGACCGGTACGACCGATACGGTGAACGTAAGACTCAGAATCCATCGGGATATCGTAGTTAACTACCa                                 >  2:3891284/1‑93 (MQ=255)
                                                          cagcGCGCGGCCAGCACGACCCGCACGACCGGTGCGACCGATACGGTGAACGTAAGACTCAGAATCCATCGGGATATCGTAGTTAACTACCAg                                <  1:5552452/93‑1 (MQ=255)
                                                          cagcGCGCGGCCAGCACGACCCGCACGACCGGTGCGACCGATACGGTGAACGTAAGACTCAGAATCCATCGGGATATCGTAGTTAACTACCAg                                <  2:2797246/93‑1 (MQ=255)
                                                           agcGCGCGGCAAGCACGACCCGCGCGACCGGCGCGACCGATTCGGGGCGCGGAAGACTCAGAATCCATCGGGATATCGTAGTTAACGACCAgg                               <  1:4286639/93‑1 (MQ=255)
                                                            gcgcgcGGCCAGCACGACCCGCACGACCGGTGCGACCGATACGGTGAACGTAAGACTCAGAATCCATCGGGATATCGTAGTTAACTACCAGGc                              <  2:575277/93‑1 (MQ=255)
                                                              gcgcgGCCAGCACGACCCGCACGACCGGTGCGACCGATACGGTGAACGTAAGACTCAGAATCCATCGGGATATCGTAGTTAACTACCAGGCTg                            >  2:5546944/1‑93 (MQ=255)
                                                               cgcgGCCAGCACGACCCGCACGACCGGTGCGACCGATACGGTGAACGTAAGACTCAGAATCCATCGGGATATCGTAGTTAACTACCAGGCTGa                           <  1:5114813/93‑1 (MQ=255)
                                                                    ccAGCACGACCCGCACGACCGGTGCGACCGATACGGTGAACGTAAGACTCAGAATCCATCGGGATATCGTAGTTAACTACCAGGCTGATACGc                      <  2:2456514/93‑1 (MQ=255)
                                                                      aGCACGACCCGCACGACCGGTGCGACCGATACGGTGAACGTAAGACTCAGAATCCATCGGGATATCGTAGTTAACTACCAGGCTGATACGCTc                    >  2:2349521/1‑93 (MQ=255)
                                                                      aGCACGACCCGCACGACCGGTGCGACCGATACGGTGAACGTAAGACTCAGAATCCATCGGGATATCGTAGTTAACTACCAGGCTGATACGCTc                    >  1:1854630/1‑93 (MQ=255)
                                                                       gCACGACCCGCACGACCGGTGCGACCGATACGGTGAACGTAAGACTCAGAATCCATCGGGATATCGTAGTTAACTACCAGGCTGATACGCTCa                   <  1:7334326/93‑1 (MQ=255)
                                                                        cACGACCCGCACGACCGGTGCGACCGATACGGTGAACGTAAGACTCAGAATCCATCGGGATATCGTAGTTAACTACCAGGCTGATACGCTCaa                  <  1:1776984/93‑1 (MQ=255)
                                                                        cACGACCCGCACGACCGGTACGACCGATACGGTGAACGTAAGACTCAGAATCCATCGGGATATCGTAGTTAACTACCAGGCTGATACGCTCaa                  <  2:1841239/93‑1 (MQ=255)
                                                                         aCGACCCGCACGACCGGTGCGACCGATACGGTGAACGTAAGACTCAGAATCCATCGGGATATCGTAGTTAACTACCAGGCTGATACGCTCAAc                 >  1:7061290/1‑93 (MQ=255)
                                                                           gACCCGCACGACCGGTGCGACCGATACGGTGAACGTAAGACTCAGAATCCATCGGGATATCGTAGTTAACTACCAGGCTGATACGCTCAACGt               <  2:4041277/93‑1 (MQ=255)
                                                                           gACCCGCACGACCGGTACGACCGATACGGTGAACGTAAGACTCAGAATCCATCGGGATATCGTAGTTAACTACCAGGCTGATACGCTCAACGt               >  1:6125336/1‑93 (MQ=255)
                                                                           gACCCGCACGACCGGTACGACCGATACGGTGAACGTAAGACTCAGAATCCATCGGGATATCGTAGTTAACTACCAGGCTGATACGCTCAACGt               <  1:5015366/93‑1 (MQ=255)
                                                                            aCCCGCACGACCGGTGCGACCGATACGGTGAACGTAAGACTCAGAATCCATCGGGATATCGTAGTTAACTACCAGGCTGATACGCTCAACGTc              >  2:590408/1‑93 (MQ=255)
                                                                              ccGCACGACCGGTGCGACCGATACGGTGAACGTAAGACTCAGAATCCATCGGGATATCGTAGTTAACTACCAGGCTGATACGCTCAACGTCCa            <  1:547857/93‑1 (MQ=255)
                                                                              ccGCACGACCGGTGCGACCGATACGGTGAACGTAAGACTCAGAATCCATCGGGATATCGTAGTTAACTACCAGGCTGATACGCTCAACGTCCa            <  2:1763519/93‑1 (MQ=255)
                                                                              ccGCACGACCGGTGCGACCGATACGGTGAACGTAAGACTCAGAATCCATCGGGATATCGTAGTTAACTACCAGGCTGATACGCTCAACGTCCa            <  2:5413946/93‑1 (MQ=255)
                                                                              ccGCACGACCGGTGCGACCGATACGGTGAACGTAAGACTCAGAATCCATCGGGATATCGTAGTTAACTACCAGGCTGATACGCTCAACGTCCa            <  1:4236766/93‑1 (MQ=255)
                                                                                gCACGACCGGTGCGACCGATACGGTGAACGTAAGCCTCAGAATCCATCGGGATATCGTAGTTAACTACCAGGCTGATACGCTCAACGTCCCgg          >  1:2992380/1‑93 (MQ=255)
                                                                                gCACGACCGGTGCGACCGATACGGTGAACGTAAGACTCAGAATCCATCGGGATATCGTAGTTAACTACCAGGCTGATACGCTCAACGTCCAgg          >  2:5355831/1‑93 (MQ=255)
                                                                                gCACGACCGGTGCGACCGATACGGTGAACGTAAGACTCAGAATCCATCGGGATATCGTAGTTAACTACCAGGCTGATACGCTCAACGTCCAgg          <  2:6940053/93‑1 (MQ=255)
                                                                                    gACCGGTACGACCGATACGGTGAACGTAAGACTCAGAATCCATCGGGATATCGTAGTTAACTACCAGGCTGATACGCTCAACGTCCAGGCCAc      >  2:4710681/1‑93 (MQ=255)
                                                                                     aCCGGTGCGACCGATACGGTGAACGTAAGACTCAGAATCCATCGGGATATCGTAGTTAACTACCAGGCTGATACGCTCAACGTCCAGGCCACg     >  2:5837079/1‑93 (MQ=255)
                                                                                     aCCGGTACGACCGATACGGTGAACGTAAGACTCAGAATCCATCGGGATATCGTAGTTAACTCCCAGGCTGATACGCTCAACTTCCAGGCCACg     <  2:4655303/93‑1 (MQ=255)
                                                                                      ccGGTGCGACCGATACGGTGAACGTAAGACTCAGAATCCATCGGGATATCGTAGTTAACTACCAGGCTGATACGCTCAACGTCCAGGCCACgg    <  1:6036223/93‑1 (MQ=255)
                                                                                       cGGTGCGACCGATACGGTGAACGTAAGACTCAGAATCCATCGGGATATCGTAGTTAACTACCAGGCTGATACGCTCAACGTCCAGGCCACggg   >  2:5316566/1‑93 (MQ=255)
                                                                                       cGGTGCGACCGATACGGTGAACGTAAGACTCAGAATCCATCGGGATATCGTAGTTAACTACCAGGCTGATACGCTCAACGTCCAGGCCACggg   >  2:4245746/1‑93 (MQ=255)
                                                                                        ggTGCGACCGATACGGTGAACGTAAGACTCAGAATCCATCGGGATATCGTAGTTAACTACCAGGCTGATACGCTCAACGTCCAGGCCACGGGc  <  2:4420867/93‑1 (MQ=255)
                                                                                           |                                                                                         
GCTTCATAGTACGTTCAATGTTGCGCAGCAGACGACGCTCGCGGTTCTCAACGAACAGCAGCGCGCGGCCAGCACGACCCGCACGACCGGTACGACCGATACGGTGAACGTAAGACTCAGAATCCATCGGGATATCGTAGTTAACTACCAGGCTGATACGCTCAACGTCCAGGCCACGGGC  >  NC_000913/3304406‑3304586

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CAGCTTCATAGTACGTTCAATGTTGCGCAGCAGACGACGCTCGCGGTTCTCAACGAACAGCAGCGCGCGGCCAGCACGACCCGCACGACCGGTACGACCGATACGGTGAACGTAAGACTCAGAATCCATCGGGATATCGTAGTTAACTACCAGGCTGATACGCTCAACGTCCAGGCCACGGGCTG  >  NC_000913/3304404‑3304588
                                                                                             |                                                                                           
CAGCTTCATAGTACGTTCAATGTTGCGCAGCAGACGACGCTCGCGGTTCTCAACGAACAGCAGCGCGCGGCCAGCACGACCCGCACGACCGGTG                                                                                             >  SRR4302542.1980680/1‑94 (MQ=60)
CAGCTTCATAGTACGTTCAATGTTGCGCAGCAGACGACGCTCGCGGTTCTCAACGAACAGCAGCGCGCGGCCAGCACGACCCGCACGACCGGTG                                                                                             >  SRR4302542.450441/1‑94 (MQ=60)
CAGCTTCATAGTACGTTCAATGTTGCGCAGCAGACGACGCTCGCGGTTCTCAACGAACAGCAGCGCGCGGCCAGCACGACCCGCACGACCGGTG                                                                                             <  SRR4302542.4908515/94‑1 (MQ=60)
 AGCTTCATAGTACGTTCAATGTTGCGCAGCAGACGACGCTCGCGGTTCTCAACGAACAGCAGCGCGCGGCCAGCACGACCCGCACGACCGGTGC                                                                                            >  SRR4302542.512344/1‑94 (MQ=60)
  GCTTCATAGTACGTTCAATGTTGCGCAGCAGACGACGCTCGCGGTTCTCAACGAACAGCAGCGCGCGGCCAGCACGACCCGCACGACCGGTGCG                                                                                           >  SRR4302542.1964690/1‑94 (MQ=60)
  GCTTCATAGTACGTTCAATGTTGCGCAGCAGACGACGCTCGCGGTTCTCAACGAACAGCAGCGCGCGGCCAGCACGACCCGCACGACCGGTGCG                                                                                           <  SRR4302542.5350119/94‑1 (MQ=60)
  GCTTCATAGTACGTTCAATGTTGCGCAGCAGACGACGCTCGCGGTTCTCAACGAACAGCAGCGCGCGGCCAGCACGACCCGCACGACCGGTGCG                                                                                           >  SRR4302542.2529420/1‑94 (MQ=60)
  GCTTCATAGTACGTTCAATGTTGCGCAGCAGACGACGCTCGCGGTTCTCAACGAACAGCAGCGCGCGGCCAGCACGACCCGCACGACCGGTGCG                                                                                           <  SRR4302542.1722442/94‑1 (MQ=60)
  GCTTCATAGTACGTTCAATGTTGCGCAGCAGACGACGCTCGCGGTTCTCAACGAACAGCAGCGCGCGGCCAGCACGACCCGCACGACCGGTACG                                                                                           <  SRR4302542.3303363/94‑1 (MQ=60)
    TTCATAGTACGTTCAATGTTGCGCAGCAGACGACGCTCGCGGTTCTCAACGAACAGCAGCGCGCGGCCAGCACGACCCGCACGACCGGTGCGAC                                                                                         >  SRR4302542.3947189/1‑94 (MQ=60)
    TTCATAGTACGTTCAATGTTGCGCAGCAGACGACGCTCGCGGTTCTCAACGAACAGCAGCGCGCGGCCAGCACGACCCGCACGACCGGTACGAC                                                                                         >  SRR4302542.2217072/1‑94 (MQ=60)
    TTCATAGTACGTTCAATGTTGCGCAGCAGACGACGCACGCGGTTCTCAACGAACAGCAGCGCGCGGCCAGCACGACCCGCACGACCGGTGCGAC                                                                                         <  SRR4302542.5751520/94‑1 (MQ=60)
     TCATAGTACGTTCAATGTTGCGCAGCAGACGACGCTCGCGGTTCTCAACGAACAGCAGCGCGCGGCCAGCGCGACCCGCACGACCGGTGCGACC                                                                                        <  SRR4302542.5046778/94‑1 (MQ=60)
     TCATAGTACGTTCAATGTTGCGCAGCAGACGACGCTCGCGGTTCTCAACGAACAGCAGCGCGCGGCCAGCACGACCCGCACGACCGGTGCGACC                                                                                        <  SRR4302542.1969195/94‑1 (MQ=60)
     TCATAGTACGTTCAATGTTGCGCAGCAGACGACGCTCGCGGTTCTCAACGAACAGCAGCGCGCGGCCAGCACGACCCGCACGACCGGTACGACC                                                                                        <  SRR4302542.1490137/94‑1 (MQ=60)
     TCATAGTACGTTCAATGTTGCGCAGCAGACGACGATCGCGGTTCTCAACGAACAGCAGCGCGCGGCCAGCACGACCCGCACGACCGGTGCGACC                                                                                        >  SRR4302542.2417746/1‑94 (MQ=60)
      CATAGTACGTTCAATGTTGCGCAGCAGACGACGCTCGCGGTTCTCAACGAACAGCAGCGCGCGGCCAGCACGACCCGCACGACCGGTGCGACCG                                                                                       <  SRR4302542.4292458/94‑1 (MQ=60)
      CATAGTACGTTCAATGTTGCGCAGCAGACGACGCTCGCGGTTCTCAACGAACAGCAGCGCGCGGCCAGCACGACCCGCACGACCGGTACGACCG                                                                                       >  SRR4302542.398090/1‑94 (MQ=60)
        TAGTACGTTCAATGTTGCGCAGCAGACGACGCTCGCGGTTCTCAACGAACAGCAGCGCGCGGCCAGCACGACCCGCACGACCGGTGCGACCGAT                                                                                     <  SRR4302542.61224/94‑1 (MQ=60)
         AGTACGTTCAATGTTGCGCAGCAGACGACGCTCGCGGTTCTCAACGAACAGCAGCGCGCGGCCAGCACGACCCGCACGACCGGTGCGACCGATA                                                                                    <  SRR4302542.7068967/94‑1 (MQ=60)
         AGTACGTTCAATGTTGCGCAGCAGACGACGCTCGCGGTTCTCAACGAACAGCAGCGCGCGGCCAGCACGACCCGCACGACCGGTGCGACCGATA                                                                                    >  SRR4302542.2799851/1‑94 (MQ=60)
         AGTACGTTCAATGTTGCGCAGCAGACGACGCTCGCGGTTCTCAACGAACAGCAGCGCGCGGCCAGCACGACCCGCACGACCGGTGCGACCGATA                                                                                    >  SRR4302542.7417242/1‑94 (MQ=60)
         AGTACGTTCAATGTTGCGCAGCAGACGACGCTCGCGGTTCTCAACGAACAGCAGCGCGCGGCCAGCACGACCCGCACGACCGGTGCGACCGATA                                                                                    >  SRR4302542.3978140/1‑94 (MQ=60)
          GTACGTTCAATGTTGCGCAGCAGACGACGCTCGCGGTTCTCAACGAACAGCAGCGCGCGGCCAGCACGACCCGCACGACCGGTGCGACCGATAC                                                                                   <  SRR4302542.3628181/94‑1 (MQ=60)
          GTACGTTCAATGTTGCGCAGCAGACGACGCTCGCGGTTCTCAACGAACAGCAGCGCGCGGCCAGCACGACCCGCACGACCGGTGCGACCGATAC                                                                                   >  SRR4302542.883557/1‑94 (MQ=60)
           TACGTTCAATGTTGCGCAGCAGACGACGCTCGCGGTTCTCAACGAACAGCAGCGCGCGGCCAGCACGACCCGCACGACCGGTGCGACCGATACG                                                                                  <  SRR4302542.7208321/94‑1 (MQ=60)
           TACGTTCAATGTTGCGCAGCAGACGACGCTCGCGGTTCTCAACGAACAGCAGCGCGCGGCCAGCACGACCCGCACGACCGGTGCGACCGATACG                                                                                  <  SRR4302542.4061561/94‑1 (MQ=60)
           TACGTTCAATGTTGCGCAGCAGACGACGCTCGCGGTTCTCAACGAACAGCAGCGCGCGGCCAGCACGACCCGCACGACCGGTGCGACCGATACG                                                                                  >  SRR4302542.6965533/1‑94 (MQ=60)
           TACGTTCAATGTTGCGCAGCAGACGACGCTCGCGGTTCTCAACGAACAGCAGCGCGCGGCCAGCACGACCCGCACGACCGGTGCGACCGATACG                                                                                  >  SRR4302542.1897518/1‑94 (MQ=60)
           TACGTTCAATGTTGCGCAGCAGACGACGCTCGCGGTTCTCAACGAACAGCAGCGCGCGGCCAGCACGACCCGCACGACCGGTGCGACCGATACG                                                                                  >  SRR4302542.5151104/1‑94 (MQ=60)
            ACGTTCAATGTTGCGCAGCAGACGACGCTCGCGGTTCTCAACGAACAGCAGCGCGCGGCCAGCACGACCCGCACGACCGGTGCGACCGATACGG                                                                                 >  SRR4302542.2712586/1‑94 (MQ=60)
            ACGTTCAATGTTGCGCAGCAGACGACGCTCGCGGTTCTCAACGAACAGCAGCGCGCGGCCAGCACGACCCGCACGACCGGTGCGACCGATACGG                                                                                 >  SRR4302542.3983520/1‑94 (MQ=60)
             CGTTCAATGTTGCGCAGCAGACGACGCTCGCGGTTCTCAACGAACAGCAGCGCGCGGCCAGCACGACCCGCACGACCGGTGCGACCGATACGGT                                                                                >  SRR4302542.55768/1‑94 (MQ=60)
              GTTCAATGTTGCGCAGCAGACGACGCTCGCGGTTCTCAACGAACAGCAGCGCGCGGCCAGCACGACCCGCACGACCGGTACGACCGATACGGTG                                                                               <  SRR4302542.3633515/94‑1 (MQ=60)
              GTTCAATGTTGCGCAGCAGACGACGCTCGCGGTTCTCAACGAACAGCAGCGCGCGGCCAGCACGACCCGCACGACCGGTACGACCGATACGGTG                                                                               <  SRR4302542.2052075/94‑1 (MQ=60)
               TTCAATGTTGCGCAGCAGACGACGCTCGCGGTTCTCAACGAACAGCAGCGCGCGGCCAGCACGACCCGCACGACCGGTGCGACCGATACGGTGA                                                                              >  SRR4302542.1683681/1‑94 (MQ=60)
                 CAATGTTGCGCAGCAGACGACGCTCGCGGGTCTCAACGAACAGCAGCGCGCGGCCAGCACGACCCGCACGACCGGGGCGACCGATACGGTGAAC                                                                            >  SRR4302542.1158901/1‑94 (MQ=60)
                  AATGTTGCGCAGCAGACGACGCTCGCGGTTCTCAACGAACAGCAGCGCGCGGCCAGCACGACCCGCACGACCGGTGCGACCGATACGGTGAACG                                                                           <  SRR4302542.2208696/94‑1 (MQ=60)
                  AATGTTGCGCAGCAGACGACGCTCGCGGTTCTCAACGAACAGCAGCGCGCGGCCAGCACGACCCGCACGACCGGTGCGACCGATACGGTGAACG                                                                           <  SRR4302542.2906589/94‑1 (MQ=60)
                  AATGTTGCGCAGCAGACGACGCTCGCGGTTCTCAACGAACAGCAGCGCGCGGCCAGCACGACCCGCACGACCGGTACGACCGATACGGTGAACG                                                                           >  SRR4302542.4241238/1‑94 (MQ=60)
                   cgatagccgcgctcacgcCGCTCGCGGTTCTCAACGAACAGCAGCGCGCGGCCAGCACGACCCGCACGACCGGTGCGACCGATACGGTGAACGT                                                                          >  SRR4302542.7587217/19‑94 (MQ=60)
                    TGTTGCGCAGCAGACGACGCTCGCGGTTCTCAACGAACAGCAGCGCGCGGCCAGCACGACCCGCACGACCGGTGCGACCGATACGGTGAACGTA                                                                         >  SRR4302542.2780546/1‑94 (MQ=60)
                    TGTTGCGCAGCAGACGACGCTCGCGGTTCTCAACGAACAGCAGCGCGCGGCCAGCACGACCCGCACGACCGGTGCGACCGATACGGTGAACGTA                                                                         >  SRR4302542.7153912/1‑94 (MQ=60)
                       TGCGCAGCAGACGACGCTCGCGGTTCTCAACGAACAGCAGCGCGCGGCCAGCACGACCCGCACGACCGGTGCGACCGATACGGTGAACGTAAGA                                                                      <  SRR4302542.304724/94‑1 (MQ=60)
                         CGCAGCAGACGACGCTCGCGGTTCTCAACGAACAGCAGCGCGCGGCCAGCACGACCCGCACGACCGGTGCGACCGATACGGTGAACGTAAGACT                                                                    >  SRR4302542.2460464/1‑94 (MQ=60)
                         CGCAGCAGACGACGCTCGCGGTTCTCAACGAACAGCAGCGCGCGGCCAGCACGACCCGCACGACCGGTGCGACCGATACGGTGAACGTAAGACT                                                                    <  SRR4302542.1469771/94‑1 (MQ=60)
                          GCAGCAGACGACGCTCGCGGTTCTCAACGAACAGCAGCGCGCGGCCAGCACGACCCGCACGACCGGTGCGACCGATACGGTGAACGTAAGACTC                                                                   >  SRR4302542.5010965/1‑94 (MQ=60)
                          GCAGCAGACGACGCTCGCGGTTCTCAACGAACAGCAGCGCGCGGCCAGCACGACCCGCACGACCGGTGCGACCGATACGGTGAACGTAAGACTC                                                                   >  SRR4302542.6823589/1‑94 (MQ=60)
                          GCAGCAGACGACGCTCGCGGTTCTCAACGAACAGCAGCGCGCGGCCAGCACGACCCGCACGACCGGTACGACCGATACGGTGAACGTAAGACTC                                                                   <  SRR4302542.3761094/94‑1 (MQ=60)
                            agcagcgcacgcccgcgtTTCTCAACAAACAGCCGCGCGCGCCCAGCACCACCCGCACGCCCGGGGCGACAGATACGGTGAACGTAAGACTCAG                                                                 <  SRR4302542.6023981/76‑1 (MQ=31)
                              CAGACGACGCTCGCGGTTCTCAACGAACAGCAGCGCGCGGCCAGCACGACCCGCACGACCGGTGCGACCGATACGGTGAACGTAAGACTCAGAA                                                               <  SRR4302542.1024379/94‑1 (MQ=60)
                               AGACGACGCTCGCGGTTCTCAACGAACAGCAGCGCGCGGCCAGCACGACCCGCACGACCGGTGCGACCGATACGGTGAACGTAAGACTCAGAAT                                                              <  SRR4302542.7316487/94‑1 (MQ=60)
                               AGACGACGCTCGCGGTTCTCAACGAACAGCAGCGCGCGGCCAGCACGACCCGCACGACCGGTGCGACCGATACGGTGAACGTAAGACTCAGAAT                                                              >  SRR4302542.4115673/1‑94 (MQ=60)
                               AGACGACGCTCGCGGTTCTCAACGAACAGCAGCGCGCGGCCAGCACGACCCGCACGACCGGTGCGACCGATACGGTGAACGTAAGACTCAGAAT                                                              >  SRR4302542.6094895/1‑94 (MQ=60)
                               AGACGACGCTCGCGGTTCTCAACGAACAGCAGCGCGCGGCCAGCACGACCCGCACGACCGGTGCGACCGATACGGTGAACGTAAGACTCAGAAT                                                              <  SRR4302542.894665/94‑1 (MQ=60)
                               AGACGACGCTCGCGGTTCTCAACGAACAGCAGCGCGCGGCCAGCACGACCCGCACGACCGGTGCGACCGATACGGTGAACGTAAGACTCAGAAT                                                              <  SRR4302542.311560/94‑1 (MQ=60)
                                GACGACGCTCGCGGTTCTCAACGAACAGCAGCGCGCGGCCAGCACGACCCGCACGACCGGTGCGACCGATACGGTGAACGTAAGACTCAGAATC                                                             >  SRR4302542.2367730/1‑94 (MQ=60)
                                GACGACGCTCGCGGTTCTCAACGAACAGCAGCGCGCGGCCAGCACGACCCGCACGACCGGTGCGACCGATACGGTGAACGTAAGACTCAGAATC                                                             <  SRR4302542.488434/94‑1 (MQ=60)
                                 ACGACGCTCGCGGTTCTCAACGAACAGCAGCGCGCGGCCAGCACGACCCGCACGACCGGTGCGACCGATACGGTGAACGTAAGACTCAGAATCC                                                            <  SRR4302542.305345/94‑1 (MQ=60)
                                 ACGACGCTCGCGGTTCTCAACGAACAGCAGCGCGCGGCCAGCACGACCCGCACGACCGGTACGACCGATACGGTGAACGTAAGACTCAGAATCC                                                            <  SRR4302542.7186249/94‑1 (MQ=60)
                                    ACGCTCGCGGTTCTCAACGAACAGCAGCGCGCGGCCAGCACGACCCGCACGACCGGTGCGACCGATACGGTGAACGTAAGACTCAGAATCCATC                                                         <  SRR4302542.1857329/94‑1 (MQ=60)
                                    ACGCTCGCGGTTCTCAACGAACAGCAGCGCGCGGCCAGCACGACCCGCACGACCGGTGCGACCGATACGGTGAACGTAAGACTCAGAATCCATC                                                         <  SRR4302542.728010/94‑1 (MQ=60)
                                     CGCTCGCGGTTCTCAACGAACAGCAGCGCGCGGCCAGCACGACCCGCACGACCGGTGCGACCGATACGGTGAACGTAAGACTCAGAATCCATCG                                                        <  SRR4302542.4938751/94‑1 (MQ=60)
                                     CGCTCGCGGTTCTCAACGAACAGCAGCGCGCGGCCAGCACGACCCGCACGACCGGTGCGACCGATACGGTGAACGTAAGACTCAGAATCCATCG                                                        <  SRR4302542.869148/94‑1 (MQ=60)
                                     CGCTCGCGGTTCTCAACGAACAGCAGCGCGCGGCCAGCACGACCCGCACGACCGGTGCGACCGATACGGTGAACGTAAGACTCAGAATCCATCG                                                        <  SRR4302542.5557532/94‑1 (MQ=60)
                                      GCTCGCGGTTCTCAACGAACAGCAGCGCGCGGCCAGCACGACCCGCACGACCGGTGCGACCGATACGGTGAACGTAAGACTCAGAATCCATCGG                                                       >  SRR4302542.5637037/1‑94 (MQ=60)
                                      GCTCGCGGTTCTCAACGAACAGCAGCGCGCGGCCAGCACGACCCGCACGACCGGTGCGACCGATACGGTGAACGTAAGACTCAGAATCCATCGG                                                       <  SRR4302542.4175144/94‑1 (MQ=60)
                                      GCTCGCGGTTCTCAACGAACAGCAGCGCGCGGCCAGCACGACCCGCACGACCGGTGCGACCGATACGGTGAACGTAAGACTCAGAATCCATCGG                                                       >  SRR4302542.551783/1‑94 (MQ=60)
                                       CTCGCGGTTCTCAACGAACAGCAGCGCGCGGCCAGCACGACCCGCACGACCGGTGCGACCGATACGGTGAACGTAAGACTCAGAATCCATCGGG                                                      >  SRR4302542.7320630/1‑94 (MQ=60)
                                       CTCGCGGTTCTCAACGAACAGCAGCGCGCGGCCAGCACGACCCGCACGACCGGTGCGACCGATACGGTGAACGTAAGACTCAGAATCCATCGGG                                                      >  SRR4302542.6606491/1‑94 (MQ=60)
                                       CTCGCGGTTCTCAACGAACAGCAGCGCGCGGCCAGCACGACCCGCACGACCGGTACGACCGATACGGTGAACGTAAGACTCAGAATCCATCGGG                                                      <  SRR4302542.2535902/94‑1 (MQ=60)
                                       CTCGCGGTTCTCAACGAACAGCAGCGCGCGGCCAGCACGACCCGCACGACCGGTACGACCGATACGGTGAACGTAAGACTCAGAATCCATCGGG                                                      <  SRR4302542.2457596/94‑1 (MQ=60)
                                        TCGCGGTTCTCAACGAACAGCAGCGCGCGGCCAGCACGACCCGCACGACCGGTGCGACCGATAACGCGAACGTAAGACTCAGAATCCATCGGGA                                                     >  SRR4302542.7534884/1‑94 (MQ=60)
                                         CGCGGTTCTCAACGAACAGCAGCGCGCGGCCAGCACGACCCGCACGACCGGTGCGACCGATACGGTGAACGTAAGACTCAGAATCCATCGGGAT                                                    <  SRR4302542.874080/94‑1 (MQ=60)
                                         CGCGGTTCTCAACGAACAGCAGCGCGCGGCCAGCACGACCCGCACGACCGGTGCGACCGATACGGTGAACGTAAGACTCAGAATCCATCGGGAT                                                    >  SRR4302542.470547/1‑94 (MQ=60)
                                           CGGTTCTCAACGAACAGCAGCGCGCGGCCAGCACGACCCGCACGACCGGTGCGACCGATACGGTGAACGTAAGACTCAGAATCCATCGGGATAT                                                  <  SRR4302542.6405905/94‑1 (MQ=60)
                                           CGGTTCTCAACGAACAGCAGCGCGCGGCCAGCACGACCCGCACGACCGGTGCGACCGATACGGTGAACGTAAGACTCAGAATCCATCGGGATAT                                                  <  SRR4302542.6660342/94‑1 (MQ=60)
                                           CGGTTCTCAACGAACAGCAGCGCGCGGCCAGCACGACCCGCACGACCGGTGCGACCGATACGGTGAACGTAAGACTCAGAATCCATCGGGATAT                                                  <  SRR4302542.3365023/94‑1 (MQ=60)
                                            GGTTCTCAACGAACAGCAGCGCGCGGCCAGCACGACCCGCACGACCGGTGCGACCGATACGGTGAACGTAAGACTCAGAATCCATCGGGATATC                                                 <  SRR4302542.3627598/94‑1 (MQ=60)
                                            GGTTCTCAACGAACAGCAGCGCGCGGCCAGCACGACCCGCACGACCGGTGCGACCGATACGGTGAACGTAAGACTCAGAATCCATCGGGATATC                                                 >  SRR4302542.2049547/1‑94 (MQ=60)
                                            GGTTCTCAACGAACAGCAGCGCGCGGCCAGCACGACCCGCACGACCGGTGCGACCGATACGGTGAACGTAAGACTCAGAATCCATCGGGATATC                                                 >  SRR4302542.6458186/1‑94 (MQ=60)
                                            GGTTCTCAACGAACAGCAGCGCGCGGCCAGCACGACCCGCACGACCGGTGCGACCGATACGGTGAACGTAAGACTCAGAATCCATCGGGATATC                                                 >  SRR4302542.4841102/1‑94 (MQ=60)
                                            GGTTCTCAACGAACAGCAGCGCGCGGCCAGCACGACCCGCACGACCGGTACGACCGATACGGTGAACGTAAGACTCAGAATCCATCGGGATATC                                                 <  SRR4302542.5646216/94‑1 (MQ=60)
                                             GTTCTCAACGAACAGCAGCGCGCGGCCAGCACGACCCGCACGACCGGTGCGACCGATACGGTGAACGTAAGACTCAGAATCCATCGGGATATCG                                                >  SRR4302542.5647172/1‑94 (MQ=60)
                                             GTTCTCAACGAACAGCAGCGCGCGGCCAGCACGACCCGCACGACCGGTACGACCGATACGGTGAACGTAAGACTCAGAATCCATCGGGATATCG                                                >  SRR4302542.3321573/1‑94 (MQ=60)
                                              TTCTCAACGAACAGCAGCGCGCGGCCAGCACGACCCGCACGACCGGTGCGACCGATACGGTGAACGTAAGACTCAGAATCCATCGGGATATCGT                                               >  SRR4302542.2614155/1‑94 (MQ=60)
                                              TTCTCAACGAACAGCAGCGCGCGGCCAGCACGACCCGCACGACCGGTGCGACCGATACGGTGAACGTAAGACTCAGAATCCATCGGGATATCGT                                               >  SRR4302542.866500/1‑94 (MQ=60)
                                              TTCTCAACGAACAGCAGCGCGCGGCCAGCACGACCCGCACGACCGGTGCGACCGATACGGTGAACGTAAGACTCAGAATCCATCGGGATATCGT                                               <  SRR4302542.4547731/94‑1 (MQ=60)
                                              TTCTCAACGAACAGCAGCGCGCGGCCAGCACGACCCGCACGACCGGTGCGACCGATACGGTGAACGTAAGACTCAGAATCCATCGGGATATCGT                                               >  SRR4302542.6582724/1‑94 (MQ=60)
                                              TTCTCAACGAACAGCAGCGCGCGGCCAGCACGACCCGCACGACCGGTGCGACCGATACGGTGAACGTAAGACTCAGAATCCATCGGGATATCGT                                               >  SRR4302542.4131176/1‑94 (MQ=60)
                                                CTCAACGAACAGCAGCGCGCGGCCAGCACGACCCGCACGACCGGTGCGACCGATACGGTGAACGTAAGACTCAGAATCCATCGGGATATCGTAG                                             <  SRR4302542.1847663/94‑1 (MQ=60)
                                                CTCAACGAACAGCAGCGCGCGGCCAGCACGACCCGCACGACCGGTGCGACCGATACGGTGAACGTAAGACTCAGAATCCATCGGGATATCGTAG                                             >  SRR4302542.2870933/1‑94 (MQ=60)
                                                CTCAACGAACAGCAGCGCGCGGCCAGCACGACCCGCACGACCGGTGCGACCGATACGGTGAACGTAAGACTCAGAATCCATCGGGATATCGTAG                                             <  SRR4302542.4040303/94‑1 (MQ=60)
                                                CTCAACGAACAGCAGCGCGCGGCCAGCACGACCCGCACGACCGGTGCGACCGATACGGTGAACGTAAGACTCAGAATCCATCGGGATATCGTAG                                             >  SRR4302542.866927/1‑94 (MQ=60)
                                                CTCAACGAACAGCAGCGCGCGGCCAGCACGACCCGCACGACCGGTGCGACCGATACGGTGAACGTAAGACTCAGAATCCATCGGGATATCGTAG                                             >  SRR4302542.3530563/1‑94 (MQ=60)
                                                CTCAACGAACAGCAGCGCGCGGCCAGCACGACCCGCACGACCGGTGCGACCGATACGGTGAACGTAAGACTCAGAATCCATCGGGATATCGTAG                                             >  SRR4302542.3813361/1‑94 (MQ=60)
                                                CTCAACGAACAGCAGCGCGCGGCCAGCACGACCCGCACGACCGGTGCGACCGATACGGTGAACGTAAGACTCAGAATCCATCGGGATATCGTAG                                             >  SRR4302542.4324825/1‑94 (MQ=60)
                                                 TCAACGAACAGCAGCGCGCGGCCAGCACGACCCGCACGACCGGTGCGACCGATACGGTGAACGTAAGACTCAGAATCCATCGGGATATCGTAGT                                            >  SRR4302542.6519613/1‑94 (MQ=60)
                                                  CAACGAACAGCAGCGCGCGGCCAGCACGACCCGCACGACCGGTGCGACCGATACGGTGAACGTAAGACTCAGAATCCATCGGGATATCGTAGTT                                           >  SRR4302542.4492828/1‑94 (MQ=60)
                                                  CAACGAACAGCAGCGCGCGGCCAGCACGACCCGCACGACCGGTGCGACCGATACGGTGAACGTAAGACTCAGAATCCATCGGGATATCGTAGTT                                           >  SRR4302542.6394890/1‑94 (MQ=60)
                                                  CAACGAACAGCAGCGCGCGGCCAGCACGACCCGCACGACCGGTACGACCGATACGGTGAACGTAAGACTCAGAATCCATCGGGATATCGTAGTT                                           >  SRR4302542.1944241/1‑94 (MQ=60)
                                                  CAACGAACAGCAGCGCGCGGCCAGCACGACCCGCACGACCGGTACGACCGATACGGTGAACGTAAGACTCAGAATCCATCGGGATATCGTAGTT                                           >  SRR4302542.1567784/1‑94 (MQ=60)
                                                     CGAACAGCAGCGCGCGGCCAGCACGACCCGCACGACCGGTGCGACCGATACGGTGAACGTAAGACTCAGAATCCATCGGGATATCGTAGTTAAC                                        <  SRR4302542.7094529/94‑1 (MQ=60)
                                                     CGAACAGCAGCGCGCGGCCAGCACGACCCGCACGACCGGTGCGACCGATACGGTGAACGTAAGACTCAGAATCCATCGGGATATCGTAGTTAAC                                        >  SRR4302542.5469737/1‑94 (MQ=60)
                                                     CGAACAGCAGCGCGCGGCCAGCACGACCCGCACGACCGGTGCGACCGATACGGTGAACGTAAGACTCAGAATCCATCGGGATATCGTAGTTAAC                                        <  SRR4302542.2128350/94‑1 (MQ=60)
                                                      GAACAGCAGCGCGCGGCCAGCACGACCCGCACGACCGGTGCGACCGATACGGTGAACGTAAGACTCAGAATCCATCGGGATATCGTAGTTAACT                                       <  SRR4302542.119283/94‑1 (MQ=60)
                                                      GAACAGCAGCGCGCGGCCAGCACGACCCGCACGACCGGTGCGACCGATACGGTGAACGTAAGACTCAGAATCCATCGGGATATCGTAGTTAACT                                       <  SRR4302542.6780647/94‑1 (MQ=60)
                                                      GAACAGCAGCGCGCGGCCAGCACGACCCGCACGACCGGTGCGACCGATACGGTGAACGTAAGACTCAGAATCCATCGGGATATCGTAGTTAACT                                       <  SRR4302542.4937241/94‑1 (MQ=60)
                                                      GAACAGCAGCGCGCGGCCAGCACGACCCGCACGACCGGTACGACCGATACGGTGAACGTAAGACTCAGAATCCATCGGGATATCGTAGTTAACT                                       <  SRR4302542.5127792/94‑1 (MQ=60)
                                                       AACAGCAGCGCGCGGCCAGCACGACCCGCACGACCGGTGCGACCGATACGGTGAACGTAAGACTCAGAATCCATCGGGATATCGTAGTTAACTA                                      >  SRR4302542.2432818/1‑94 (MQ=60)
                                                        ACAGCAGCGCGCGGCCAGCACGACCCGCACGACCGGTGCGACCGATACGGTGAACGTAAGACTCAGAATCCATCGGGATATCGTAGTTAACTAC                                     <  SRR4302542.7125427/94‑1 (MQ=60)
                                                        ACAGCAGCGCGCGGCCAGCACGACCCGCACGACCGGTGCGACCGATACGGTGAACGTAAGACTCAGAATCCATCGGGATATCGTAGTTAACTAC                                     <  SRR4302542.3989307/94‑1 (MQ=60)
                                                        ACAGCAGCGCGCGGCCAGCACGACCCGCACGACCGGTACGACCGATACGGTGAACGTAAGACTCAGAATCCATCGGGATATCGTAGTTAACTAC                                     <  SRR4302542.161173/94‑1 (MQ=60)
                                                          AGCAGCGCGCGGCCAGCACGACCCGCACGACCGGTGCGACCGATACGGTGAACGTAAGACTCAGAATCCATCGGGATATCGTAGTTAACTACCA                                   >  SRR4302542.400794/1‑94 (MQ=60)
                                                           GCAGCGCGCGGCCAGCACGACCCGCACGACCGGTGCGACCGATACGGTGAACGTAAGACTCAGAATCCATCGGGATATCGTAGTTAACTACCAG                                  <  SRR4302542.4223854/94‑1 (MQ=60)
                                                            CAGCGCGCGGCCAGCACGACCCGCACGACCGGTGCGACCGATACGGTGAACGTAAGACTCAGAATCCATCGGGATATCGTAGTTAACTACCAGG                                 <  SRR4302542.5606405/94‑1 (MQ=60)
                                                            CAGCGCGCGGCCAGCACGACCCGCACGACCGGTGCGACCGATACGGTGAACGTAAGACTCAGAATCCATCGGGATATCGTAGTTAACTACCAGG                                 <  SRR4302542.5017446/94‑1 (MQ=60)
                                                             AGCGCGCGGCCAGCACGACCCGCACGACCGGTGCGACCGATACGGTGAACGTAAGACTCAGAATCCATCGGGATATCGTAGTTAACTACCAGGC                                >  SRR4302542.4169920/1‑94 (MQ=60)
                                                             AGCGCGCGGCCAGCACGACCCGCACGACCGGTGCGACCGATACGGTGAACGTAAGACTCAGAATCCATCGGGATATCGTAGTTAACTACCAGGC                                >  SRR4302542.1875637/1‑94 (MQ=60)
                                                             AGCGCGCGGCAAGCACGACCCGCGCGACCGGCGCGACCGATTCGGGGCGCGGAAGACTCAGAATCCATCGGGATATCGTAGTTAACGACCAGGC                                <  SRR4302542.4328802/94‑1 (MQ=42)
                                                                 CGCGGCCAGCACGACCCGCACGACCGGTGCGACCGATACGGTGAACGTAAGACTCAGAATCCATCGGGATATCGTAGTTAACTACCAGGCTGAT                            <  SRR4302542.4074865/94‑1 (MQ=60)
                                                                 CGCGGCCAGCACGACCCGCACGACCGGTGCGACCGATACGGTGAACGTAAGACTCAGAATCCATCGGGATATCGTAGTTAACTACCAGGCTGAT                            <  SRR4302542.5164492/94‑1 (MQ=60)
                                                                   CGGCCAGCACGACCCGCACGACCGGTGCGACCGATACGGTGAACGTAAGACTCAGAATCCATCGGGATATCGTAGTTAACTACaatatcccgat                          <  SRR4302542.6295799/94‑12 (MQ=60)
                                                                   CGGCCAGCACGACCCGCACGACCGGTGCGACCGATACGGTGAACGTAAGACTCAGAATCCATCGGGATATCGTAGTTAACTACCAGGCTGATAC                          <  SRR4302542.2034526/94‑1 (MQ=60)
                                                                    GGCCAGCACGACCCGCACGACCGGTGCGACCGATACGGTGAACGTAAGACTCAGAATCCATCGGGATATCGTAGTTAACTACCAGGCTGATACG                         <  SRR4302542.4044961/94‑1 (MQ=60)
                                                                       CAGCACGACCCGCACGACCGGTGCGACCGATACGGTGAACGTAAGACTCAGAATCCATCGGGATATCGTAGTTAACTACCAGGCTGATACGCTC                      <  SRR4302542.7370692/94‑1 (MQ=60)
                                                                       CAGCACGACCCGCACGACCGGTGCGACCGATACGGTGAACGTAAGACTCAGAATCCATCGGGATATCGTAGTTAACTACCAGGCTGATACGCTC                      >  SRR4302542.1870664/1‑94 (MQ=60)
                                                                       CAGCACGACCCGCACGACCGGTGCGACCGATACGGTGAACGTAAGACTCAGAATCCATCGGGATATCGTAGTTAACTACCAGGCTGATACGCTC                      <  SRR4302542.7112947/94‑1 (MQ=60)
                                                                        AGCACGACCCGCACGACCGGTGCGACCGATACGGTGAACGTAAGACTCAGAATCCATCGGGATATCGTAGTTAACTACCAGGCTGATACGCTCA                     >  SRR4302542.821743/1‑94 (MQ=60)
                                                                         GCACGACCCGCACGACCGGTGCGACCGATACGGTGAACGTAAGACTCAGAATCCATCGGGATATCGTAGTTAACTACCAGGCTGATACGCTCAA                    <  SRR4302542.7406174/94‑1 (MQ=60)
                                                                          CACGACCCGCACGACCGGTGCGACCGATACGGTGAACGTAAGACTCAGAATCCATCGGGATATCGTAGTTAACTACCAGGCTGATACGCTCAAC                   <  SRR4302542.1792383/94‑1 (MQ=60)
                                                                          CACGACCCGCACGACCGGTGCGACCGATACGGTGAACGTAAGACTCAGAATCCATCGGGATATCGTAGTTAACTACCAGGCTGATACGCTCAAC                   >  SRR4302542.7130598/1‑94 (MQ=60)
                                                                          CACGACCCGCACGACCGGTGCGACCGATACGGTGAACGTAAGACTCAGAATCCATCGGGATATCGTAGTTAACTACCAGGCTGATACGCTCAAC                   >  SRR4302542.1542258/1‑94 (MQ=60)
                                                                            CGACCCGCACGACCGGTGCGACCGATACGGTGAACGTAAGACTCAGAATCCATCGGGATATCGTAGTTAACTACCAGGCTGATACGCTCAACGT                 <  SRR4302542.5698942/94‑1 (MQ=60)
                                                                            CGACCCGCACGACCGGTGCGACCGATACGGTGAACGTAAGACTCAGAATCCATCGGGATATCGTAGTTAACTACCAGGCTGATACGCTCAACGT                 >  SRR4302542.6833631/1‑94 (MQ=60)
                                                                            CGACCCGCACGACCGGTACGACCGATACGGTGAACGTAAGACTCAGAATCCATCGGGATATCGTAGTTAACTACCAGGCTGATACGCTCAACGT                 >  SRR4302542.6184607/1‑94 (MQ=60)
                                                                             GACCCGCACGACCGGTACGACCGATACGGTGAACGTAAGACTCAGAATCCATCGGGATATCGTAGTTAACTACCAGGCTGATACGCTCAACGTC                <  SRR4302542.5064077/94‑1 (MQ=60)
                                                                               CCCGCACGACCGGTGCGACCGATACGGTGAACGTAAGACTCAGAATCCATCGGGATATCGTAGTTAACTACCAGGCTGATACGCTCAACGTCCA              >  SRR4302542.4078656/1‑94 (MQ=60)
                                                                                CCGCACGACCGGTGCGACCGATACGGTGAACGTAAGACTCAGAATCCATCGGGATATCGTAGTTAACTACCAGGCTGATACGCTCAACGTCCAG             <  SRR4302542.552585/94‑1 (MQ=60)
                                                                                CCGCACGACCGGTGCGACCGATACGGTGAACGTAAGACTCAGAATCCATCGGGATATCGTAGTTAACTACCAGGCTGATACGCTCAACGTCCAG             <  SRR4302542.7409139/94‑1 (MQ=60)
                                                                                CCGCACGACCGGTGCGACCGATACGGTGAACGTAAGACTCAGAATCCATCGGGATATCGTAGTTAACTACCAGGCTGATACGCTCAACGTCCAG             <  SRR4302542.4278488/94‑1 (MQ=60)
                                                                                CCGCACGACCGGTGCGACCGATACGGTGAACGTAAGACTCAGAATCCATCGGGATATCGTAGTTAACTACCAGGCTGATACGCTCAACGTCCAG             <  SRR4302542.4320866/94‑1 (MQ=60)
                                                                                CCGCACGACCGGTGCGACCGATACGGTGAACGTAAGACTCAGAATCCATCGGGATATCGTAGTTAACTACCAGGCTGATACGCTCAACGTCCAG             <  SRR4302542.4439842/94‑1 (MQ=60)
                                                                                 CGCACGACCGGTGCGACCGATACGGTGAACGTAAGCCTCAGAATCCATCGGGATATCGTAGTTAACTACCAGGCTGATACGCTCAACGTCCCGG            >  SRR4302542.3021017/1‑94 (MQ=60)
                                                                                 CGCACGACCGGTGCGACCGATACGGTGAACGTAAGACTCAGAATCCATCGGGATATCGTAGTTAACTACCAGGCTGATACGCTCAACGTCCAGG            >  SRR4302542.7227947/1‑94 (MQ=60)
                                                                                 CGCACGACCGGTGCGACCGATACGGTGAACGTAAGACTCAGAATCCATCGGGATATCGTAGTTAACTACCAGGCTGATACGCTCAACGTCCAGG            >  SRR4302542.3021018/1‑94 (MQ=60)
                                                                                   CACGACCGGTGCGACCGATACGGTGAACGTAAGACTCAGAATCCATCGGGATATCGTAGTTAACTACCAGGCTGATACGCTCAACGTCCAGGCC          >  SRR4302542.3727579/1‑94 (MQ=60)
                                                                                   CACGACCGGTGCGACCGATACGGTGAACGTAAGACTCAGAATCCATCGGGATATCGTAGTTAACTACCAGGCTGATACGCTCAACGTCCAGGCC          >  SRR4302542.146451/1‑94 (MQ=60)
                                                                                    ACGACCGGTGCGACCGATACGGTGAACGTAAGACTCAGAATCCATCGGGATATCGTAGTTAACTACCAGGCTGATACGCTCAACGTCCAGGCCA         >  SRR4302542.6607651/1‑94 (MQ=60)
                                                                                     CGACCGGTGCGACCGATACGGTGAACGTAAGACTCAGAATCCATCGGGATATCGTAGTTAACTACCAGGCTGATACGCTCAACGTCCAGGCCAC        >  SRR4302542.1771197/1‑94 (MQ=60)
                                                                                      GACCGGTGCGACCGATACGGTGAACGTAAGACTCAGAATCCATCGGGATATCGTAGTTAACTACCAGGCTGATACGCTCAACGTCCAGGCCACG       <  SRR4302542.6976177/94‑1 (MQ=60)
                                                                                       ACCGGTGCGACCGATACGGTGAACGTAAGACTCAGAATCCATCGGGATATCGTAGTTAACTACCAGGCTGATACGCTCAACGTCCAGGCCACGG      <  SRR4302542.3280187/94‑1 (MQ=60)
                                                                                        CCGGTGCGACCGATACGGTGAACGTAAGACTCAGAATCCATCGGGATATCGTAGTTAACTACCAGGCTGATACGCTCAACGTCCAGGCCACGGG     <  SRR4302542.4326370/94‑1 (MQ=60)
                                                                                        CCGGTGCGACCGATACGGTGAACGTAAGACTCAGAATCCATCGGGATATCGTAGTTAACTACCAGGCTGATACGCTCAACGTCCAGGCCACGGG     <  SRR4302542.6094659/94‑1 (MQ=60)
                                                                                           GTGCGACCGATACGGTGAACGTAAGACTCAGAATCCATCGGGATATCGTAGTTAACTACCAGGCTGATACGCTCAACGTCCAGGCCACGGGCTG  >  SRR4302542.2681707/1‑94 (MQ=60)
                                                                                           GTGCGACCGATACGGTGAACGTAAGACTCAGAATCCATCGGGATATCGTAGTTAACTACCAGGCTGATACGCTCAACGTCCAGGCCACGGGCTG  >  SRR4302542.2630951/1‑94 (MQ=60)
                                                                                           GTGCGACCGATACGGTGAACGTAAGACTCAGAATCCATCGGGATATCGTAGTTAACTACCAGGCTGATACGCTCAACGTCCAGGCCACGGGCTG  <  SRR4302542.5079064/94‑1 (MQ=60)
                                                                                           GTGCGACCGATACGGTGAACGTAAGACTCAGAATCCATCGGGATATCGTAATTAACTACCAGGCTGATACGCTCAACGTCCAGGCCACGGGCTG  >  SRR4302542.5329007/1‑94 (MQ=60)
                                                                                             |                                                                                           
CAGCTTCATAGTACGTTCAATGTTGCGCAGCAGACGACGCTCGCGGTTCTCAACGAACAGCAGCGCGCGGCCAGCACGACCCGCACGACCGGTACGACCGATACGGTGAACGTAAGACTCAGAATCCATCGGGATATCGTAGTTAACTACCAGGCTGATACGCTCAACGTCCAGGCCACGGGCTG  >  NC_000913/3304404‑3304588

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑