Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1061 294.1 15065038 98.6% 14854127 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,430,453 +C coding (745/747 nt) smf ← putative Rossmann‑fold nucleotide‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,430,4531.C99.6% 777.3 / ‑3.7 230coding (745/747 nt)smfputative Rossmann‑fold nucleotide‑binding protein
Reads supporting (aligned to +/- strand):  ref base . (0/1);  new base C (122/107);  total (122/108)
Fisher's exact test for biased strand distribution p-value = 4.70e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.90e-01

Only 100 of 247 total aligned reads displayed.

GAATTTCTTCCGGTTCCGTCACAAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGG‑AAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCGCTTCCACCACCAGTACACCTT  >  NC_000913/3430361‑3430539
                                                                                             |                                                                                      
gAATTTCTTCCGGTTCCGTCACAAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAgg                                                                                         >  2:5457923/1‑93 (MQ=255)
 aaTTTCTTCCGGTTCCGTCACAAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAggc                                                                                        >  2:7114816/1‑92 (MQ=255)
  aTTTCTTCCGGTTCCGTCACAAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAggca                                                                                       >  1:3793736/1‑91 (MQ=255)
  aTTTCTTCCGGTTCCGTCACAAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAggca                                                                                       >  2:1944268/1‑91 (MQ=255)
   tttCTTCCGGTTCCGTCACAAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTggcaagg‑caa                                                                                     >  2:330139/1‑93 (MQ=255)
   tttCTTCCGGTTCCGTCACAAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTggcaagg‑caa                                                                                     >  2:7100758/1‑93 (MQ=255)
    ttcttcCGGTTCCGTCACAAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGG‑caaa                                                                                    <  1:4750134/93‑1 (MQ=255)
    ttcttcCGGTTCCGTCACAAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGG‑caaa                                                                                    <  1:1002982/93‑1 (MQ=255)
    ttcttcCGGTTCCGTCACAAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGG‑caaa                                                                                    >  1:5341094/1‑93 (MQ=255)
      cttcCGGTTCCGTCACAAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGG‑caaaaa                                                                                  <  1:1188801/93‑2 (MQ=255)
      cttcCGGTTCCGTCACAAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCCTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGG‑caaaaa                                                                                  <  1:7287750/93‑2 (MQ=255)
       ttcCGGTTCCGTCACAAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAAc                                                                                  <  1:427381/93‑1 (MQ=255)
         ccGGTTCCGTCACAAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACtt                                                                                >  1:6473156/1‑93 (MQ=255)
           ggTTCCGTCACAAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTtct                                                                              <  1:2454527/93‑1 (MQ=255)
           ggTTCCGTCACAAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTtct                                                                              <  1:1844919/93‑1 (MQ=255)
            gTTCCGTCACAAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTtctc                                                                             <  2:1293682/93‑1 (MQ=255)
             ttCCGTCACAAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCg                                                                            >  2:550312/1‑93 (MQ=255)
             ttCCGTCACAAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCg                                                                            >  2:5910382/1‑93 (MQ=255)
             ttCCGTCACAAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCACg                                                                            >  1:7092866/1‑93 (MQ=255)
              tCCGTCACAAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGc                                                                           <  2:77035/93‑1 (MQ=255)
              tCCGTCACAAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGc                                                                           <  2:3370212/93‑1 (MQ=255)
               ccGTCACAAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGTCCTGGCAAGGCAAAAACTTCTCGcc                                                                          <  2:5810060/93‑1 (MQ=255)
                cGTCACAAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGccc                                                                         >  2:313083/1‑93 (MQ=255)
                 gTCACAAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGcccc                                                                        <  1:2716115/93‑1 (MQ=255)
                 gTCACAAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGcccc                                                                        <  2:487241/93‑1 (MQ=255)
                 gTCACAAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGcccc                                                                        <  2:444578/93‑1 (MQ=255)
                  tCACAAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCt                                                                       >  1:168003/1‑93 (MQ=255)
                  tCACAAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCt                                                                       >  2:3617983/1‑93 (MQ=255)
                   cacaAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTg                                                                      >  1:6922255/1‑93 (MQ=255)
                   cacaAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTg                                                                      <  1:2008159/93‑1 (MQ=255)
                    acaAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGc                                                                     >  1:4666937/1‑93 (MQ=255)
                     caAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCt                                                                    >  2:2295833/1‑93 (MQ=255)
                      aaGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTc                                                                   >  2:378994/1‑93 (MQ=255)
                       aGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCa                                                                  >  2:4529226/1‑93 (MQ=255)
                       aGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCa                                                                  >  1:4360234/1‑93 (MQ=255)
                        gAATCGCGCCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCaa                                                                 <  1:2204040/93‑1 (MQ=255)
                          aTCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTAGCCCCTGCTCAAgc                                                               >  2:16488/1‑93 (MQ=255)
                            cGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAgcgc                                                             <  2:2132909/93‑1 (MQ=255)
                             gCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGca                                                            >  1:5308398/1‑93 (MQ=255)
                             gCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGca                                                            <  1:7260166/93‑1 (MQ=255)
                              cACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGcac                                                           >  1:4862481/1‑93 (MQ=255)
                              cACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGcac                                                           <  2:1397831/93‑1 (MQ=255)
                                 cTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAAc                                                        >  1:4454194/1‑93 (MQ=255)
                                   tGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGt                                                      <  2:6166664/93‑1 (MQ=255)
                                   tGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGt                                                      <  1:4423466/93‑1 (MQ=255)
                                    gTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTg                                                     <  2:2728162/93‑1 (MQ=255)
                                    gTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTg                                                     >  2:5602695/1‑93 (MQ=255)
                                    gTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTg                                                     >  2:5393291/1‑93 (MQ=255)
                                     ttttATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGc                                                    <  1:3247412/93‑1 (MQ=255)
                                      tttATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCt                                                   >  1:6761038/1‑93 (MQ=255)
                                        tattaACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGt                                                 <  2:4779182/93‑1 (MQ=255)
                                        tattaACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGt                                                 <  1:7435919/93‑1 (MQ=255)
                                         attaACCGGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTc                                                <  1:3354/93‑1 (MQ=255)
                                           taACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCAc                                              <  2:2522378/93‑1 (MQ=255)
                                           taACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCAc                                              <  2:6647022/93‑1 (MQ=255)
                                            aaCCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCAcc                                             >  1:2551776/1‑93 (MQ=255)
                                             aCCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCa                                            <  1:3203694/93‑1 (MQ=255)
                                             aCCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCa                                            <  1:6629670/93‑1 (MQ=255)
                                             aCCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCa                                            >  1:7520984/1‑93 (MQ=255)
                                               cAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGc                                          >  1:5724503/1‑93 (MQ=255)
                                               cAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGc                                          >  2:6721019/1‑93 (MQ=255)
                                                  tGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCACGCGCACAACGTGTTGTCACCAGCGaa                                       >  2:4700639/1‑93 (MQ=255)
                                                  tGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGaa                                       >  2:1171478/1‑93 (MQ=255)
                                                   gAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAAc                                      >  2:6320658/1‑93 (MQ=255)
                                                    aGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAAcc                                     <  2:2906567/93‑1 (MQ=255)
                                                        ccTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTa                                 <  2:4998716/93‑1 (MQ=255)
                                                        ccTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTa                                 <  2:1076718/93‑1 (MQ=255)
                                                         cTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTAc                                <  2:6086600/93‑1 (MQ=255)
                                                         cTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTAc                                >  1:1597440/1‑93 (MQ=255)
                                                          ttCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACg                               >  1:3967266/1‑93 (MQ=255)
                                                          ttCGCTTCCCGGATTCCCTATTGGACCGGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACg                               >  2:6424699/1‑93 (MQ=255)
                                                           tCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGc                              >  2:2396534/1‑93 (MQ=255)
                                                            cGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCa                             >  1:6336399/1‑93 (MQ=255)
                                                             gCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCaa                            <  2:4097051/93‑1 (MQ=255)
                                                              cTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCaaa                           <  1:1127252/93‑1 (MQ=255)
                                                               ttCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAg                          >  1:3525959/1‑93 (MQ=255)
                                                                tCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGc                         >  1:5757966/1‑93 (MQ=255)
                                                                 cccGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGcc                        >  1:6117133/1‑93 (MQ=255)
                                                                  ccGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCg                       >  2:4661377/1‑93 (MQ=255)
                                                                  ccGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCg                       >  2:4310283/1‑93 (MQ=255)
                                                                  ccGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCg                       <  2:4213203/93‑1 (MQ=255)
                                                                  ccGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCg                       >  1:5217129/1‑93 (MQ=255)
                                                                   cGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCGc                      >  1:2111134/1‑93 (MQ=255)
                                                                   cGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCGc                      <  1:2829876/93‑1 (MQ=255)
                                                                    ggATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCGCt                     >  1:2052759/1‑93 (MQ=255)
                                                                    ggATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCGCt                     <  1:3990964/93‑1 (MQ=255)
                                                                      aTTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCGCTTc                   >  1:933707/1‑93 (MQ=255)
                                                                       ttCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCGCTTcc                  <  2:7376745/93‑1 (MQ=255)
                                                                       ttCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCGCTTcc                  <  1:5468042/93‑1 (MQ=255)
                                                                       ttCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCGCTTcc                  >  1:5677296/1‑93 (MQ=255)
                                                                         cccTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCGCTTccac                >  2:1815056/1‑93 (MQ=255)
                                                                              ttGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCGCTTccaccacca           >  2:5676103/1‑93 (MQ=255)
                                                                              ttGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCGCTTccaccacca           <  1:4636845/93‑1 (MQ=255)
                                                                              ttGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCGCTTccaccacca           >  2:5235685/1‑93 (MQ=255)
                                                                              ttGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCGCTTccaccacca           <  2:2658171/93‑1 (MQ=255)
                                                                               tGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCGCTTCCACCACCAg          <  2:1697199/93‑1 (MQ=255)
                                                                                ggACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTTCTGTCACCAGCGAACCACTACGCAAAGCCGCTTCCACCACCAGt         <  2:4558798/93‑1 (MQ=255)
                                                                                    cTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCGCTTCCACCACCAGTacac     >  1:5020229/1‑93 (MQ=255)
                                                                                    cTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCGCTTCCACCACCAGTacac     >  2:298880/1‑93 (MQ=255)
                                                                                    cTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCGCTTCCACCACCAGTacac     >  1:1429489/1‑93 (MQ=255)
                                                                                             |                                                                                      
GAATTTCTTCCGGTTCCGTCACAAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGG‑AAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCGCTTCCACCACCAGTACACCTT  >  NC_000913/3430361‑3430539

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AGAATTTCTTCCGGTTCCGTCACAAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAA‑GG‑AAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCGCTTCCACCACCAGTACACCTTTACTTAG  >  NC_000913/3430360‑3430546
                                                                                              |                                                                                              
AGAATTTCTTCCGGTTCCGTCACAAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAA‑GG                                                                                                <  SRR4302542.122462/94‑1 (MQ=60)
AGAATTTCTTCCGGTTCCGTCACAAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAA‑GG                                                                                                <  SRR4302542.359020/94‑1 (MQ=60)
AGAATTTCTTCCGGTTCCGTCACAAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAA‑GG                                                                                                <  SRR4302542.1255534/94‑1 (MQ=60)
  AATTTCTTCCGGTTCCGTCACAAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAA‑GG‑CA                                                                                             >  SRR4302542.3831296/1‑94 (MQ=60)
  AATTTCTTCCGGTTCCGTCACAAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAA‑GG‑CA                                                                                             <  SRR4302542.2667847/94‑1 (MQ=60)
   ATTTCTTCCGGTTCCGTCACAAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAA‑GG‑CAA                                                                                            >  SRR4302542.819005/1‑94 (MQ=60)
    TTTCTTCCGGTTCCGTCACAAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAA‑GG‑CAAA                                                                                           >  SRR4302542.5392983/1‑94 (MQ=60)
     TTCTTCCGGTTCCGTCACAAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAA‑GG‑CAAAA                                                                                          <  SRR4302542.1011619/94‑1 (MQ=60)
     TTCTTCCGGTTCCGTCACAAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAA‑GG‑CAAAA                                                                                          <  SRR4302542.4796423/94‑1 (MQ=60)
       CTTCCGGTTCCGTCACAAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAA‑GGCAAAAAC                                                                                         >  SRR4302542.4341138/1‑94 (MQ=60)
       CTTCCGGTTCCGTCACAAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAA‑GGCAAAAAC                                                                                         <  SRR4302542.1199040/94‑1 (MQ=60)
       CTTCCGGTTCCGTCACAAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAA‑GGCAAAAAC                                                                                         >  SRR4302542.5790205/1‑94 (MQ=60)
       CTTCCGGTTCCGTCACAAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCCTCGCTTCCCGGATTCCCTATTGGACCTGGCAA‑GGCAAAAAC                                                                                         <  SRR4302542.7359195/94‑1 (MQ=60)
        TTCCGGTTCCGTCACAAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAA‑GGCAAAAACT                                                                                        <  SRR4302542.431075/94‑1 (MQ=60)
         TCCGGTTCCGTCACAAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAA‑GGCAAAAACTT                                                                                       >  SRR4302542.6536932/1‑94 (MQ=60)
          CCGGTTCCGTCACAAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAA‑GGCAAAAACTTC                                                                                      <  SRR4302542.3037561/94‑1 (MQ=60)
          CCGGTTCCGTCACAAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAA‑GGCAAAAACTTC                                                                                      >  SRR4302542.2505752/1‑94 (MQ=60)
           CGGTTCCGTCACAAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAA‑GGCAAAAACTTCT                                                                                     >  SRR4302542.577247/1‑94 (MQ=60)
           CGGTTCCGTCACAAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAA‑GGCAAAAACTTCT                                                                                     <  SRR4302542.5622453/94‑1 (MQ=60)
            GGTTCCGTCACAAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAA‑GGCAAAAACTTCTC                                                                                    <  SRR4302542.1860881/94‑1 (MQ=60)
            GGTTCCGTCACAAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAA‑GGCAAAAACTTCTC                                                                                    <  SRR4302542.2478393/94‑1 (MQ=60)
             GTTCCGTCACAAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAA‑GGCAAAAACTTCTCG                                                                                   <  SRR4302542.1521387/94‑1 (MQ=60)
             GTTCCGTCACAAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAA‑GGCAAAAACTTCTCG                                                                                   <  SRR4302542.2254549/94‑1 (MQ=60)
             GTTCCGTCACAAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAA‑GGCAAAAACTTCACG                                                                                   >  SRR4302542.7162447/1‑94 (MQ=60)
              TTCCGTCACAAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAA‑GGCAAAAACTTCTCGC                                                                                  <  SRR4302542.3237959/94‑1 (MQ=60)
              TTCCGTCACAAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAA‑GGCAAAAACTTCTCGC                                                                                  >  SRR4302542.6757092/1‑94 (MQ=60)
                CCGTCACAAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAA‑GGCAAAAACTTCTCGCCC                                                                                <  SRR4302542.3320884/94‑1 (MQ=60)
                 CGTCACAAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAA‑GGCAAAAACTTCTCGCCCC                                                                               >  SRR4302542.5348326/1‑94 (MQ=60)
                  GTCACAAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAA‑GGCAAAAACTTCTCGCCCCT                                                                              <  SRR4302542.2742307/94‑1 (MQ=60)
                  GTCACAAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAA‑GGCAAAAACTTCTCGCCCCT                                                                              >  SRR4302542.169426/1‑94 (MQ=60)
                  GTCACAAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAA‑GGCAAAAACTTCTCGCCCCT                                                                              >  SRR4302542.5626259/1‑94 (MQ=60)
                   TCACAAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAA‑GGCAAAAACTTCTCGCCCCTG                                                                             >  SRR4302542.6990216/1‑94 (MQ=60)
                   TCACAAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAA‑GGCAAAAACTTCTCGCCCCTG                                                                             <  SRR4302542.3486245/94‑1 (MQ=60)
                    CACAAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAA‑GGCAAAAACTTCTCGCCCCTGC                                                                            >  SRR4302542.4712558/1‑94 (MQ=60)
                    CACAAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAA‑GGCAAAAACTTCTCGCCCCTGC                                                                            <  SRR4302542.2025505/94‑1 (MQ=60)
                      CAAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAA‑GGCAAAAACTTCTCGCCCCTGCTC                                                                          >  SRR4302542.6907631/1‑94 (MQ=60)
                       AAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAA‑GGCAAAAACTTCTCGCCCCTGCTCA                                                                         <  SRR4302542.3852907/94‑1 (MQ=60)
                       AAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAA‑GGCAAAAACTTCTCGCCCCTGCTCA                                                                         >  SRR4302542.4403048/1‑94 (MQ=60)
                        AGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAA‑GGCAAAAACTTCTCGCCCCTGCTCAA                                                                        >  SRR4302542.2080819/1‑94 (MQ=60)
                        AGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAA‑GGCAAAAACTTCTCGCCCCTGCTCAA                                                                        >  SRR4302542.7126864/1‑94 (MQ=60)
                         GAATCGCGCCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAA‑GGCAAAAACTTCTCGCCCCTGCTCAAG                                                                       <  SRR4302542.2223011/94‑1 (MQ=60)
                         GAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAA‑GGCAAAAACTTCTCGCCCCTGCTCAAG                                                                       >  SRR4302542.4854427/1‑94 (MQ=60)
                          AATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAA‑GGCAAAAACTTCTCGCCCCTGCTCAAGC                                                                      >  SRR4302542.6651274/1‑94 (MQ=60)
                          AATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCAATTGGACCTGGCAA‑GGCAAAAACTTCTCGCCCCTGCTCAAGC                                                                      >  SRR4302542.7449549/1‑94 (MQ=60)
                           ATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAA‑GGCAAAAACTTCTCGCCCCTGCTCAAGCG                                                                     <  SRR4302542.4759107/94‑1 (MQ=60)
                           ATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAA‑GGCAAAAACTTCTCGCCCCTGCTCAAGCG                                                                     <  SRR4302542.2091929/94‑1 (MQ=60)
                             CGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAA‑GGCAAAAACTTCTCGCCCCTGCTCAAGCGCA                                                                   >  SRR4302542.5359969/1‑94 (MQ=60)
                              GCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAA‑GGCAAAAACTTCTCGCCCCTGCTCAAGCGCAC                                                                  <  SRR4302542.7331342/94‑1 (MQ=60)
                              GCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAA‑GGCAAAAACTTCTCGCCCCTGCTCAAGCGCAC                                                                  >  SRR4302542.4909833/1‑94 (MQ=60)
                              GCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAA‑GGCAAAAACTTCTCGCCCCTGCTCAAGCGCAC                                                                  <  SRR4302542.7331343/94‑1 (MQ=60)
                               CACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAA‑GGCAAAAACTTCTCGCCCCTGCTCAAGCGCACA                                                                 <  SRR4302542.5507340/94‑1 (MQ=60)
                               CACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAA‑GGCAAAAACTTCTCGCCCCTGCTCAAGCGCACA                                                                 <  SRR4302542.6539803/94‑1 (MQ=60)
                                 CCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAATGC‑AAAAACTTCTCGCCCCTGCTCAAGCGCACAAC                                                               <  SRR4302542.4629603/94‑1 (MQ=60)
                                 CCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAA‑GGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAAC                                                               <  SRR4302542.6967600/94‑1 (MQ=60)
                                 CCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAA‑GGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAAC                                                               >  SRR4302542.1164803/1‑94 (MQ=60)
                                 CCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAA‑GGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAAC                                                               >  SRR4302542.4497891/1‑94 (MQ=60)
                                 CCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAA‑GGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAAC                                                               >  SRR4302542.2202990/1‑94 (MQ=60)
                                  CTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAA‑GGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACG                                                              >  SRR4302542.5929961/1‑94 (MQ=60)
                                  CTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAA‑GGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACG                                                              <  SRR4302542.4033257/94‑1 (MQ=60)
                                    TGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAA‑GGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTG                                                            >  SRR4302542.6309325/1‑94 (MQ=60)
                                    TGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAA‑GGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTG                                                            <  SRR4302542.4466909/94‑1 (MQ=60)
                                    TGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAA‑GGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTG                                                            <  SRR4302542.3880896/94‑1 (MQ=60)
                                      TTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAA‑GGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCT                                                          <  SRR4302542.4920557/94‑1 (MQ=60)
                                      TTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAA‑GGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCT                                                          >  SRR4302542.939762/1‑94 (MQ=60)
                                      TTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAA‑GGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCT                                                          <  SRR4302542.3278103/94‑1 (MQ=60)
                                      TTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAA‑GGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCT                                                          >  SRR4302542.6827543/1‑94 (MQ=60)
                                         TATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAA‑GGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTC                                                       <  SRR4302542.7508696/94‑1 (MQ=60)
                                          ATTAACCGGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAA‑GGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCA                                                      <  SRR4302542.3382/94‑1 (MQ=60)
                                            TAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAA‑GGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACC                                                    >  SRR4302542.2576509/1‑94 (MQ=60)
                                            TAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAA‑GGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACC                                                    <  SRR4302542.4693874/94‑1 (MQ=60)
                                            TAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAA‑GGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACC                                                    <  SRR4302542.1866122/94‑1 (MQ=60)
                                             AACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAA‑GGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCA                                                   >  SRR4302542.7594948/1‑94 (MQ=60)
                                             AACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAA‑GGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCA                                                   >  SRR4302542.1236716/1‑94 (MQ=60)
                                              ACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAA‑GGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAG                                                  <  SRR4302542.6695029/94‑1 (MQ=60)
                                              ACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAA‑GGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAG                                                  <  SRR4302542.3234043/94‑1 (MQ=60)
                                               CCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAA‑GGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGC                                                 <  SRR4302542.1839641/94‑1 (MQ=60)
                                               CCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAA‑GGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGC                                                 <  SRR4302542.4642956/94‑1 (MQ=60)
                                               CCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAA‑GGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGC                                                 <  SRR4302542.4107863/94‑1 (MQ=60)
                                               CCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAA‑GGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGC                                                 >  SRR4302542.5780070/1‑94 (MQ=60)
                                                  GTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAA‑GGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAA                                              >  SRR4302542.5299503/1‑94 (MQ=60)
                                                   TGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAA‑GGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAAC                                             <  SRR4302542.5068807/94‑1 (MQ=60)
                                                      GGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAA‑GGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCAC                                          >  SRR4302542.4720271/1‑94 (MQ=60)
                                                      GGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAA‑GGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCAC                                          >  SRR4302542.1882459/1‑94 (MQ=60)
                                                         CCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAA‑GGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTAC                                       >  SRR4302542.1669145/1‑94 (MQ=60)
                                                         CCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAA‑GGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTAC                                       >  SRR4302542.1611237/1‑94 (MQ=60)
                                                         CCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAA‑GGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTAC                                       <  SRR4302542.6187668/94‑1 (MQ=60)
                                                          CTTCGCTTCCCGGATTCCCTATTGGACCTGGCAA‑GGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACG                                      >  SRR4302542.2054810/1‑94 (MQ=60)
                                                          CTTCGCTTCCCGGATTCCCTATTGGACCTGGCAA‑GGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACG                                      >  SRR4302542.7547241/1‑94 (MQ=60)
                                                          CTTCGCTTCCCGGATTCCCTATTGGACCTGGCAA‑GGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACG                                      >  SRR4302542.4006431/1‑94 (MQ=60)
                                                          CTTCGCTTCCCGGATTCCCTATTGGACCTGGCAA‑GGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACG                                      >  SRR4302542.4024401/1‑94 (MQ=60)
                                                            TCGCTTCCCGGATTCCCTATTGGACCTGGCAA‑GGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCA                                    >  SRR4302542.6398827/1‑94 (MQ=60)
                                                             CGCTTCCCGGATTCCCTATTGGACCTGGCAA‑GGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAA                                   >  SRR4302542.1345843/1‑94 (MQ=60)
                                                              GCTTCCCGGATTCCCTATTGGACCTGGCAA‑GGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAA                                  >  SRR4302542.6480230/1‑94 (MQ=60)
                                                               CTTCCCGGATTCCCTATTGGACCTGGCAA‑GGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAG                                 >  SRR4302542.2780994/1‑94 (MQ=60)
                                                               CTTCCCGGATTCCCTATTGGACCTGGCAA‑GGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAG                                 <  SRR4302542.1136875/94‑1 (MQ=60)
                                                               CTTCCCGGATTCCCTATTGGACCTGGCAA‑GGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAG                                 >  SRR4302542.2688582/1‑94 (MQ=60)
                                                               CTTCCCGGATTCCCTATTGGACCTGGCAA‑GGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAG                                 >  SRR4302542.3558912/1‑94 (MQ=60)
                                                                TTCCCGGATTCCCTATTGGACCTGGCAA‑GGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGC                                >  SRR4302542.5813859/1‑94 (MQ=60)
                                                                TTCCCGGATTCCCTATTGGACCTGGCAA‑GGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGC                                <  SRR4302542.4411991/94‑1 (MQ=60)
                                                                 TCCCGGATTCCCTATTGGACCTGGCAA‑GGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCC                               <  SRR4302542.7223082/94‑1 (MQ=60)
                                                                 TCCCGGATTCCCTATTGGACCTGGCAA‑GGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCC                               >  SRR4302542.6176327/1‑94 (MQ=60)
                                                                  CCCGGATTCCCTATTGGACCTGGCAA‑GGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCG                              >  SRR4302542.5267863/1‑94 (MQ=60)
                                                                   CCGGATTCCCTATTGGACCTGGCAA‑GGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCGC                             >  SRR4302542.2129354/1‑94 (MQ=60)
                                                                    CGGATTCCCTATTGGACCTGGCAA‑GGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCGCT                            >  SRR4302542.2070500/1‑94 (MQ=60)
                                                                    CGGATTCCCTATTGGACCTGGCAA‑GGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCGCT                            <  SRR4302542.3688648/94‑1 (MQ=60)
                                                                    CGGATTCCCTATTGGACCTGGCAA‑GGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCGCT                            >  SRR4302542.7238108/1‑94 (MQ=60)
                                                                    CGGATTCCCTATTGGACCTGGCAA‑GGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCGCT                            <  SRR4302542.2857072/94‑1 (MQ=60)
                                                                     GGATTCCCTATTGGACCTGGCAA‑GGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCGCTT                           <  SRR4302542.4030341/94‑1 (MQ=60)
                                                                     GGATTCCCTATTGGACCTGGCAA‑GGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCGCTT                           <  SRR4302542.2873337/94‑1 (MQ=60)
                                                                      GATTCCCTATTGGACCTGGCAA‑GGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCGCTTC                          >  SRR4302542.941746/1‑94 (MQ=60)
                                                                       ATTCCCTATTGGACCTGGCAA‑GGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCGCTTCC                         >  SRR4302542.5732426/1‑94 (MQ=60)
                                                                        TTCCCTATTGGACCTGGCAA‑GGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCGCTTCCA                        <  SRR4302542.5521159/94‑1 (MQ=60)
                                                                          CCCTATTGGACCTGGCAA‑GGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCGCTTCCACC                      >  SRR4302542.578854/1‑94 (MQ=60)
                                                                           CCTATTGGACCTGGCAA‑GGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCGCTTCCACCA                     >  SRR4302542.5078031/1‑94 (MQ=60)
                                                                            CTATTGGACCTGGCAA‑GGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCGCTTCCACCAC                    >  SRR4302542.737405/1‑94 (MQ=60)
                                                                               TTGGACCTGGCAA‑GGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCGCTTCCACCACCAG                 <  SRR4302542.4682148/94‑1 (MQ=60)
                                                                                 GGACCTGGCAA‑GGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCGCTTCCACCACCAGTA               <  SRR4302542.110368/94‑1 (MQ=60)
                                                                                   ACCTGGCAA‑GGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTTCTGTCACCAGCGAACCACTACGCAAAGCCGCTTCCACCACCAGTACA             <  SRR4302542.3487966/94‑1 (MQ=60)
                                                                                    CCTGGCAA‑GGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCGCTTCCACCACCAGTACAC            >  SRR4302542.1653294/1‑94 (MQ=60)
                                                                                    CCTGGCAA‑GGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCGCTTCCACCACCAGTACAC            >  SRR4302542.5391310/1‑94 (MQ=60)
                                                                                    CCTGGCAA‑GGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCGCTTCCACCACCAGTACAC            >  SRR4302542.5068979/1‑94 (MQ=60)
                                                                                    CCTGGCAA‑GGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCGCTTCCACCACCAGTACAC            >  SRR4302542.1441852/1‑94 (MQ=60)
                                                                                     CTGGCAA‑GGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCGCTTCCACCACCAGTACACC           >  SRR4302542.4483047/1‑94 (MQ=60)
                                                                                      TGGCAA‑GGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCGCTTCCACCACCAGTACACCT          >  SRR4302542.966531/1‑94 (MQ=60)
                                                                                       GGCAA‑GGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCGCTTCCACCACCAGTACACCTT         >  SRR4302542.446095/1‑94 (MQ=60)
                                                                                       GGCAA‑GGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCGCTTCCACCACCAGTACACCTT         >  SRR4302542.2384916/1‑94 (MQ=60)
                                                                                        GCAA‑GGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCGCTTCCACCACCAGTACACCTTT        >  SRR4302542.6752066/1‑94 (MQ=60)
                                                                                        GCAA‑GGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCGCTTCCACCACCAGTACACCTTT        >  SRR4302542.3678544/1‑94 (MQ=60)
                                                                                         CAA‑GGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCGCTTCCACCACCAGTACACCTTTA       >  SRR4302542.225938/1‑94 (MQ=60)
                                                                                          AA‑GGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCGCTTCCACCACCAGTACACCTTTAC      >  SRR4302542.6703495/1‑94 (MQ=60)
                                                                                          AA‑GGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCGCTTCCACCACCAGTACACCTTTAC      >  SRR4302542.236880/1‑94 (MQ=60)
                                                                                          AA‑GGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCGCTTCCACCACCAGTACACCTTTAC      >  SRR4302542.284941/1‑94 (MQ=60)
                                                                                          AA‑GGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCGCTTCCACCACCAGTACACCTTTAC      <  SRR4302542.2995405/94‑1 (MQ=60)
                                                                                          AA‑GGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCGCTTCCACCACCAGTACACCTTTAC      >  SRR4302542.7448472/1‑94 (MQ=60)
                                                                                           A‑GGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCGCTTCCACCACCAGTACACCTTTACT     <  SRR4302542.4733064/94‑1 (MQ=60)
                                                                                              C‑AAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCGCTTCCACCACCAGTACACCTTTACTTAG  <  SRR4302542.3944959/94‑1 (MQ=60)
                                                                                              C‑AAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCGCTTCCACCACCAGTACACCTTTACTTAG  >  SRR4302542.801335/1‑94 (MQ=60)
                                                                                              C‑AAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCGCTTCCACCACCAGTACACCTTTACTTAG  <  SRR4302542.7382745/94‑1 (MQ=60)
                                                                                              C‑AAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCGCTTCCACCACCAGTACACCTTTACTTAG  <  SRR4302542.4248832/94‑1 (MQ=60)
                                                                                              C‑AAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCGCTTCCACCACCAGTACACCTTTACTTAG  <  SRR4302542.5173884/94‑1 (MQ=60)
                                                                                              |                                                                                              
AGAATTTCTTCCGGTTCCGTCACAAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAA‑GG‑AAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCGCTTCCACCACCAGTACACCTTTACTTAG  >  NC_000913/3430360‑3430546

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑