Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	1061	294.1	15065038	98.6%	14854127	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,477,397	T→C	N283S (AAC→AGC)	kefB ←	KefB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,477,397	0	T	C	83.6%	612.7 / 109.0	274	N283S (AAC→AGC)	kefB	KefB
Reads supporting (aligned to +/- strand): ref base T (18/27); new base C (101/128); total (119/155)
Fisher's exact test for biased strand distribution p-value = 6.27e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 276 total aligned reads displayed.

GCGCCAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCAC  >  NC_000913/3477305‑3477487

gcgcCAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGt                                                                                            <  1:5476847/93‑1 (MQ=255)

  gcCAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTg                                                                                          <  2:6346334/93‑1 (MQ=255)

  gcCAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGCTg                                                                                          >  1:5316964/1‑93 (MQ=255)

   ccAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGCTGa                                                                                         >  2:844087/1‑93 (MQ=255)

   ccAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGCTGa                                                                                         <  1:1509711/93‑1 (MQ=255)

    cagcagATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGCTGAg                                                                                        <  2:5533891/93‑1 (MQ=255)

    cagcagATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGCTGAg                                                                                        <  1:6411931/93‑1 (MQ=255)

      gcagATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTg                                                                                      >  2:5025074/1‑93 (MQ=255)

      gcagATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGCTGAGTg                                                                                      <  2:3957079/93‑1 (MQ=255)

       cagATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGCTGAGTGa                                                                                     <  2:2327888/93‑1 (MQ=255)

        agATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGCTGAGTGAc                                                                                    >  2:34276/1‑93 (MQ=255)

        agATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGCTGAGTGAc                                                                                    >  1:2638750/1‑93 (MQ=255)

        agATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGCTGAGTGAc                                                                                    <  2:3343794/93‑1 (MQ=255)

        agATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGCTGAGTGAc                                                                                    >  1:1463202/1‑93 (MQ=255)

         gATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGCTGAGTGACa                                                                                   >  2:4473478/1‑93 (MQ=255)

         gATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGCTGAGTGACa                                                                                   <  2:3772583/93‑1 (MQ=255)

         gATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGCTGAGTGACa                                                                                   >  1:2519976/1‑93 (MQ=255)

           tACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGCTGAGTGACATg                                                                                 >  1:86301/1‑93 (MQ=255)

            aCAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGCTGAGTGACATGc                                                                                <  1:1158692/93‑1 (MQ=255)

            aCAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGCTGAGTGACATGc                                                                                <  1:2774328/93‑1 (MQ=255)

              aGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGCTGAGTGACATGCCg                                                                              >  1:2279403/1‑93 (MQ=255)

              aGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGCTGAGTGACATGCCg                                                                              >  2:5342249/1‑93 (MQ=255)

               gCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGCTGAGTGACATGCCGa                                                                             >  2:1534385/1‑93 (MQ=255)

                cACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGCTGAGTGACATGCCGAc                                                                            >  1:5539528/1‑93 (MQ=255)

                 aCGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGCTGAGTGACATGCCGACa                                                                           >  2:5982339/1‑93 (MQ=255)

                 aCGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGCTGAGTGACATGCCGACa                                                                           >  2:6877254/1‑93 (MQ=255)

                  cGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGCTGAGTGACATGCCGACag                                                                          >  2:1322736/1‑93 (MQ=255)

                  cGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGCTGAGTGACATGCCGACag                                                                          >  1:3050545/1‑93 (MQ=255)

                  cGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGCTGAGTGACATGCCGACag                                                                          >  1:4160740/1‑93 (MQ=255)

                   gagaATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGCTGAGTGACATGCCGACaga                                                                         <  2:6383758/93‑1 (MQ=255)

                   gagaATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGCTGAGTGACATGCCGACaga                                                                         <  2:2115384/93‑1 (MQ=255)

                   gagaATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGCTGAGTGACATGCCGACaga                                                                         <  1:2251073/93‑1 (MQ=255)

                     gaATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGCTGAGTGACATGCCGACAgaga                                                                       <  2:666555/93‑1 (MQ=255)

                       aTTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGCTGAGTGACATGCCGACAGAGATa                                                                     >  1:6655696/1‑93 (MQ=255)

                        ttttCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGCTGAGTGACATGCCGACAGAGATaa                                                                    >  2:1889744/1‑93 (MQ=255)

                         tttCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGAGATaaa                                                                   <  2:3823790/93‑1 (MQ=255)

                         tttCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGCTGAGTGACATGCCGACAGAGATaaa                                                                   <  1:163186/93‑1 (MQ=255)

                           tCATCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGCTGAGTGACATGCCGACAGAGATAAAGa                                                                 >  1:6974308/1‑93 (MQ=255)

                           tCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGCTGAGTGACATGCCGACAGAGATAAAGa                                                                 <  1:3930603/93‑1 (MQ=255)

                            cACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGCTGAGTGACATGCCGACAGAGATAAAGaa                                                                <  2:1687578/93‑1 (MQ=255)

                             aCCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGCTGAGTGACATGCCGACAGAGATAAAGAAc                                                               <  1:1313982/93‑1 (MQ=255)

                              ccGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGCTGAGTGACATGCCGACAGAGATAAAGAACa                                                              >  2:6939276/1‑93 (MQ=255)

                                 ccaccaGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGAGATAAAGAACAAAc                                                           <  2:2716841/93‑1 (MQ=255)

                                 ccaccaGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGCTGAGTGACATGCCGACAGAGATAAAGAACAAAc                                                           >  1:4088718/1‑93 (MQ=255)

                                   accaGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGAGATAAAGAACAAACCg                                                         <  1:5119535/93‑1 (MQ=255)

                                   accaGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGCTGAGTGACATGCCGACAGAGATAAAGAACAAACCg                                                         <  1:809978/93‑1 (MQ=255)

                                    ccaGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGAGATAAAGAACAAACCGa                                                        <  2:1727795/93‑1 (MQ=255)

                                    ccaGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGCTGAGTGACATGCCGACAGAGATAAAGAACAAACCGa                                                        <  2:5940313/93‑1 (MQ=255)

                                    ccaGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGCTGAGTGACATGCCGACAGAGATAAAGAACAAACCGa                                                        >  2:1107101/1‑93 (MQ=255)

                                     caGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGCTGAGTGCCATGCCGACAGAGAAAAAGAACAAACCGag                                                       <  1:7399687/93‑1 (MQ=255)

                                      aGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGAGATAAAGAACAAACCGagc                                                      >  1:7034388/1‑93 (MQ=255)

                                       gCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGAGATAAAGAACAAACCGagca                                                     <  1:3383330/93‑1 (MQ=255)

                                        cacaACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGCTGAGTGACATGCCGACAGAGATAAAGAACAAACCGagcag                                                    >  1:2097038/1‑93 (MQ=255)

                                          caACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGCTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAgcagca                                                  >  2:4042158/1‑93 (MQ=255)

                                            aCCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGCTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAg                                                <  1:6391931/93‑1 (MQ=255)

                                               aCGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGCTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCt                                             >  1:2017376/1‑93 (MQ=255)

                                               aCGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCAAGGTTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCt                                             >  2:4943620/1‑93 (MQ=255)

                                                cGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGCTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCtt                                            <  1:6475124/93‑1 (MQ=255)

                                                cGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGCTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCtt                                            <  1:3202722/93‑1 (MQ=255)

                                                cGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGCTGAGTGACACGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCtt                                            <  1:3123869/93‑1 (MQ=255)

                                                 gCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCttt                                           >  2:7209822/1‑93 (MQ=255)

                                                  cTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTg                                          <  2:3520784/93‑1 (MQ=255)

                                                   ttATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGCTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGa                                         >  2:4197363/1‑93 (MQ=255)

                                                    tATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGaa                                        >  1:381396/1‑93 (MQ=255)

                                                    tATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGCTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGaa                                        >  1:6006436/1‑93 (MQ=255)

                                                    tATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGCTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGaa                                        <  2:6494871/93‑1 (MQ=255)

                                                      tCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGCTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAgg                                      <  2:7086201/93‑1 (MQ=255)

                                                       cACTACCCACAACAGATGGGTATAAAGCACCCCGAGGCTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAggg                                     >  2:4247615/1‑93 (MQ=255)

                                                       cACTACCCACAACAGATGGGTATAAAGCACCCCGAGGCTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAggg                                     <  1:4354127/93‑1 (MQ=255)

                                                        aCTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGa                                    <  2:2366550/93‑1 (MQ=255)

                                                        aCTACCCACAACAGATGGGTATAAAGCACCCCGAGGCTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGa                                    >  1:5610722/1‑93 (MQ=255)

                                                         cTACCCACAACAGATGGGTATAAAGCACCCCGAGGCTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGAt                                   <  1:4610181/93‑1 (MQ=255)

                                                           aCCCACAACAGATGGGTATAAAGCACCCCGAGGCTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCg                                 <  2:2027989/93‑1 (MQ=255)

                                                           aCCCACAACAGATGGGTATAAAGCACCCCGAGGCTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCg                                 <  1:6846630/93‑1 (MQ=255)

                                                             ccACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGAt                               <  2:5117780/93‑1 (MQ=255)

                                                              cacaACAGATGGGTATAAAGCACCCCGAGGCTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATa                              <  1:2456991/93‑1 (MQ=255)

                                                              cacaACAGATGGGTATAAAGCACCCCGAGGCTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATa                              <  2:4504359/93‑1 (MQ=255)

                                                                caacaGATGGGTATAAAGCACCCCGAGGCTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGc                            >  1:7329494/1‑93 (MQ=255)

                                                                caacaGATGGGTATAAAGCACCCCGAGGCTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGc                            <  2:6149906/93‑1 (MQ=255)

                                                                 aacaGATGGGTATAAAGCACCCCGAGGCTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGcc                           <  2:4729229/93‑1 (MQ=255)

                                                                    aGATGGGTATAAAGCACCCCGAGGCTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGtt                        <  2:512010/93‑1 (MQ=255)

                                                                    aGATGGGTATAAAGCACCCCGAGGCTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGtt                        <  2:4841214/93‑1 (MQ=255)

                                                                     gATGGGTATAAAGCACCCCGAGGCTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGttt                       >  1:888626/1‑93 (MQ=255)

                                                                     gATGGGTATAAAGCACCCCGAGGCTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGttt                       >  2:1365274/1‑93 (MQ=255)

                                                                      aTGGGTATAAAGCACCCCGAGGCTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTc                      <  2:3083140/93‑1 (MQ=255)

                                                                       tGGGTATAAAGCACCCCGAGGCTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTcc                     >  2:2142641/1‑93 (MQ=255)

                                                                        gggTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCa                    >  1:2907470/1‑93 (MQ=255)

                                                                         ggTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAg                   <  1:2683518/93‑1 (MQ=255)

                                                                             taAAGGACCCCGAGGCTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCa               >  1:4703695/1‑93 (MQ=255)

                                                                             taAAGCACCCCGAGGCTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCa               >  1:4550019/1‑93 (MQ=255)

                                                                              aaaGCACCCCGAGGTTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTG‑AGGGATCGATAGCCGTTTCCAGTTCATg             <  1:3462480/93‑1 (MQ=255)

                                                                              aaaGCACCCCGAGGCTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCAt              <  1:1012321/93‑1 (MQ=255)

                                                                               aaGCACCCCGAGGCTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATg             >  1:654822/1‑93 (MQ=255)

                                                                               aaGCACCCCGAGGCTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATg             >  1:3550114/1‑93 (MQ=255)

                                                                                aGCACCCCGAGGCTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATgg            >  2:2916091/1‑93 (MQ=255)

                                                                                 gCACCCCGAGGCTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGc           <  2:3294372/93‑1 (MQ=255)

                                                                                        gAGGCTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTc    >  2:3717619/1‑93 (MQ=255)

                                                                                         aGGTTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCa   <  2:5734132/93‑1 (MQ=255)

                                                                                         aGGCTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCa   <  2:2510188/93‑1 (MQ=255)

                                                                                          ggTTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCAc  <  1:3866186/93‑1 (MQ=255)

GCGCCAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCAC  >  NC_000913/3477305‑3477487

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

GCGCCAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTT  >  NC_000913/3477305‑3477490

GCGCCAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTT                                                                                              <  SRR4302542.5530051/94‑1 (MQ=60)

 CGCCAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTG                                                                                             <  SRR4302542.2093946/94‑1 (MQ=60)

 CGCCAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGCTG                                                                                             >  SRR4302542.5368616/1‑94 (MQ=60)

 CGCCAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGCTG                                                                                             <  SRR4302542.5831438/94‑1 (MQ=60)

   CCAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGCTGAG                                                                                           <  SRR4302542.2096941/94‑1 (MQ=60)

   CCAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGCTGAG                                                                                           <  SRR4302542.1522757/94‑1 (MQ=60)

    CAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGCTGAGT                                                                                          <  SRR4302542.6475115/94‑1 (MQ=60)

     AGCAGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGCTGAGTG                                                                                         <  SRR4302542.885321/94‑1 (MQ=60)

      GCAGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGCTGAGTGA                                                                                        >  SRR4302542.814645/1‑94 (MQ=60)

       CAGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGCTGAGTGAC                                                                                       >  SRR4302542.2664234/1‑94 (MQ=60)

       CAGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGCTGAGTGAC                                                                                       >  SRR4302542.1475833/1‑94 (MQ=60)

        AGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGCTGAGTGACA                                                                                      >  SRR4302542.2544419/1‑94 (MQ=60)

        AGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGCTGAGTGACA                                                                                      >  SRR4302542.2678536/1‑94 (MQ=60)

          ATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGCTGAGTGACATG                                                                                    >  SRR4302542.87020/1‑94 (MQ=60)

            ACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGCTGAGTGACATGCC                                                                                  <  SRR4302542.1168625/94‑1 (MQ=60)

            ACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGCTGAGTGACATGCC                                                                                  >  SRR4302542.1283755/1‑94 (MQ=60)

            ACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGCTGAGTGACATGCC                                                                                  <  SRR4302542.2801055/94‑1 (MQ=60)

             CAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGCTGAGTGACATGCCG                                                                                 <  SRR4302542.4775339/94‑1 (MQ=60)

             CAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGCTGAGTGACATGCCG                                                                                 >  SRR4302542.7195753/1‑94 (MQ=60)

             CAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGCTGAGTGACATGCCG                                                                                 >  SRR4302542.2298993/1‑94 (MQ=60)

              AGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGCTGAGTGACATGCCGA                                                                                >  SRR4302542.1768131/1‑94 (MQ=60)

               GCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGCTGAGTGACATGCCGAC                                                                               >  SRR4302542.5593374/1‑94 (MQ=60)

                CACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGCTGAGTGACATGCCGACA                                                                              >  SRR4302542.3563345/1‑94 (MQ=60)

                 ACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGCTGAGTGACATGCCGACAG                                                                             >  SRR4302542.4201717/1‑94 (MQ=60)

                 ACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGCTGAGTGACATGCCGACAG                                                                             >  SRR4302542.3079664/1‑94 (MQ=60)

                 ACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGCTGAGTGACATGCCGACAG                                                                             >  SRR4302542.4584300/1‑94 (MQ=60)

                   GAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGAG                                                                           >  SRR4302542.3155934/1‑94 (MQ=60)

                   GAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGCTGAGTGACATGCCGACAGAG                                                                           <  SRR4302542.2270436/94‑1 (MQ=60)

                    AGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGCTGAGTGACATGCCGACAGAGA                                                                          <  SRR4302542.1519506/94‑1 (MQ=60)

                      AATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGCTGAGTGACATGCCGACAGAGATA                                                                        <  SRR4302542.3078594/94‑1 (MQ=60)

                      AATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGCTGAGTGACATGCCGACAGAGATA                                                                        >  SRR4302542.6721279/1‑94 (MQ=60)

                      AATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGCTGAGTGACATGCCGACAGAGATA                                                                        <  SRR4302542.5484603/94‑1 (MQ=60)

                       ATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGCTGAGTGACATGCCGACAGAGATAA                                                                       >  SRR4302542.6473302/1‑94 (MQ=60)

                         TTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGAGATAAAG                                                                     <  SRR4302542.4856104/94‑1 (MQ=60)

                         TTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGCTGAGTGACATGCCGACAGAGATAAAG                                                                     <  SRR4302542.4670618/94‑1 (MQ=60)

                         TTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGCTGAGTGACATGCCGACAGAGATAAAG                                                                     <  SRR4302542.164567/94‑1 (MQ=60)

                         TTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGCTGAGTGACATGCCGACAGAGATAAAG                                                                     <  SRR4302542.6846057/94‑1 (MQ=60)

                          TTCATCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGCTGAGTGACATGCCGACAGAGATAAAGA                                                                    >  SRR4302542.7042814/1‑94 (MQ=60)

                          TTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGAGATAAAGA                                                                    <  SRR4302542.2961708/94‑1 (MQ=60)

                          TTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGCTGAGTGACATGCCGACAGAGATAAAGA                                                                    <  SRR4302542.2743051/94‑1 (MQ=60)

                          TTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGCTGAGTGACATGCCGACAGAGATAAAGA                                                                    <  SRR4302542.867428/94‑1 (MQ=60)

                           TCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGCTGAGTGACATGCCGACAGAGATAAAGAA                                                                   <  SRR4302542.3969388/94‑1 (MQ=60)

                            CACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGCTGAGTGACATGCCGACAGAGATAAAGAAC                                                                  >  SRR4302542.2120522/1‑94 (MQ=60)

                             CCCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGCTGAGTGACATGCCGACAGAGATAAAGAACA                                                                 <  SRR4302542.1220261/94‑1 (MQ=60)

                             ACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGCTGAGTGACATGCCGACAGAGATAAAGAACA                                                                 >  SRR4302542.2584784/1‑94 (MQ=60)

                             ACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGCTGAGTGACATGCCGACAGAGATAAAGAACA                                                                 >  SRR4302542.3759832/1‑94 (MQ=60)

                             ACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGCTGAGTGACATGCCGACAGAGATAAAGAACA                                                                 <  SRR4302542.1325305/94‑1 (MQ=60)

                             ACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGCTGAGTGACATGCCGACAGAGATAAAGAACA                                                                 <  SRR4302542.846186/94‑1 (MQ=60)

                               CGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGCTGAGTGACATGCCGACAGAGATAAAGAACAAA                                                               >  SRR4302542.2489801/1‑94 (MQ=60)

                                GCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGCTGAGTGACATGCCGACAGAGATAAAGAACAAAC                                                              >  SRR4302542.4129048/1‑94 (MQ=60)

                                 CCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGAGATAAAGAACAAACC                                                             >  SRR4302542.7215872/1‑94 (MQ=60)

                                  CACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGCTGAGTGACATGCCGACAGAGATAAAGAACAAACCG                                                            <  SRR4302542.3318445/94‑1 (MQ=60)

                                   ACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGAGATAAAGAACAAACCGA                                                           <  SRR4302542.5169250/94‑1 (MQ=60)

                                   ACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGCTGAGTGACATGCCGACAGAGATAAAGAACAAACCGA                                                           <  SRR4302542.816968/94‑1 (MQ=60)

                                    CCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAG                                                          >  SRR4302542.5575083/1‑94 (MQ=60)

                                     CAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGC                                                         >  SRR4302542.7103496/1‑94 (MQ=60)

                                     CAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGCTGAGTGCCATGCCGACAGAGAAAAAGAACAAACCGAGC                                                         <  SRR4302542.7472099/94‑1 (MQ=60)

                                     CAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGCTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGC                                                         >  SRR4302542.1501244/1‑94 (MQ=60)

                                      AGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGTGGCTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCA                                                        >  SRR4302542.7445760/1‑94 (MQ=60)

                                       GCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAG                                                       <  SRR4302542.3415106/94‑1 (MQ=60)

                                       GCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGCTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAG                                                       >  SRR4302542.2115136/1‑94 (MQ=60)

                                       GCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGCTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAG                                                       <  SRR4302542.4333754/94‑1 (MQ=60)

                                           AACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAG                                                   >  SRR4302542.1331181/1‑94 (MQ=60)

                                           AACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGCTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAG                                                   >  SRR4302542.4160154/1‑94 (MQ=60)

                                            ACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGCTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGC                                                  <  SRR4302542.6454902/94‑1 (MQ=60)

                                              CACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGCTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCT                                                <  SRR4302542.5595638/94‑1 (MQ=60)

                                              CACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGCTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCT                                                >  SRR4302542.2034825/1‑94 (MQ=60)

                                              CACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGCTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCT                                                <  SRR4302542.2469123/94‑1 (MQ=60)

                                               ACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTT                                               >  SRR4302542.887605/1‑94 (MQ=60)

                                                CGCTTATCACTACCCACAACAGATTGGTATAAAGCACCCCGAGGCTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTT                                              >  SRR4302542.4325319/1‑94 (MQ=60)

                                                CGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGCTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTT                                              <  SRR4302542.1708453/94‑1 (MQ=60)

                                                CGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGCTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTT                                              <  SRR4302542.6538919/94‑1 (MQ=60)

                                                CGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGCTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTT                                              <  SRR4302542.3233061/94‑1 (MQ=60)

                                                CGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGCTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTT                                              <  SRR4302542.320293/94‑1 (MQ=60)

                                                CGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGCTGAGTGACACGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTT                                              <  SRR4302542.3153584/94‑1 (MQ=60)

                                                 GCTTATCACTCCCCACAACAGATGGGTATAAAGCACCCCGAGGCTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTG                                             <  SRR4302542.6520248/94‑1 (MQ=60)

                                                 GCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGCTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTG                                             <  SRR4302542.2530049/94‑1 (MQ=60)

                                                 CCTTATCACTTCCCACAACAGATGGGTATAAAGCCCCCCGAGGCTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTG                                             <  SRR4302542.5328354/94‑1 (MQ=60)

                                                  CTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGA                                            <  SRR4302542.2203354/94‑1 (MQ=60)

                                                   TTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAA                                           >  SRR4302542.384712/1‑94 (MQ=60)

                                                   TTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGCTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAA                                           <  SRR4302542.2605925/94‑1 (MQ=60)

                                                   TTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGCTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAA                                           >  SRR4302542.6064605/1‑94 (MQ=60)

                                                    TATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAG                                          <  SRR4302542.3872468/94‑1 (MQ=60)

                                                    TATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGCTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAG                                          <  SRR4302542.3917616/94‑1 (MQ=60)

                                                    TATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGCTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAG                                          >  SRR4302542.6592351/1‑94 (MQ=60)

                                                     ATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGCTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGG                                         >  SRR4302542.6259916/1‑94 (MQ=60)

                                                     ATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGCTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGG                                         <  SRR4302542.6700767/94‑1 (MQ=60)

                                                       CACTACCCACAACAGATGGGTATAAAGCACCCCGAGGCTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGA                                       >  SRR4302542.5665232/1‑94 (MQ=60)

                                                       CACTACCCACAACAGATGGGTATAAAGCACCCCGAGGCTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGA                                       <  SRR4302542.1430838/94‑1 (MQ=60)

                                                       CACTACCCACAACAGATGGGTATAAAGCACCCCGAGGCTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGA                                       <  SRR4302542.4396903/94‑1 (MQ=60)

                                                        ACTACCCACAACAGATGGGTATAAAGCACCCCGAGGCTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGAT                                      >  SRR4302542.910110/1‑94 (MQ=60)

                                                        ACTACCCACAACAGATGGGTATAAAGCACCCCGAGGCTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGAT                                      <  SRR4302542.344286/94‑1 (MQ=60)

                                                         CTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATC                                     <  SRR4302542.6553379/94‑1 (MQ=60)

                                                         CTACCCACAACAGATGGGTATAAAGCACCCCGAGGCTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATC                                     <  SRR4302542.5406609/94‑1 (MQ=60)

                                                         CTACCCACAACAGATGGGTATAAAGCACCCCGAGGCTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATC                                     <  SRR4302542.4655235/94‑1 (MQ=60)

                                                         CTACCCACAACAGATGGGTATAAAGCACCCCGAGGCTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATC                                     >  SRR4302542.2866410/1‑94 (MQ=60)

                                                         CTACCCACAACAGATGGGTATAAAGCACCCCGAGGCTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATC                                     >  SRR4302542.6647485/1‑94 (MQ=60)

                                                          TACCCACAACAGATGGGTATAAAGCACCCCGAGGCTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCG                                    >  SRR4302542.7086952/1‑94 (MQ=60)

                                                          TACCCACAACAGATGGGTATAAAGCACCCCGAGGCTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCG                                    <  SRR4302542.4032041/94‑1 (MQ=60)

                                                           ACCCACAACAGATGGGTATAAAGCACCCCGAGGCTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGA                                   >  SRR4302542.2270598/1‑94 (MQ=60)

                                                           ACCCACAACAGATGGGTATAAAGCACCCCGAGGCTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGA                                   <  SRR4302542.6913878/94‑1 (MQ=60)

                                                            CCCACAACAGATGGGTATAAAGCACCCCGAGGCTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGAT                                  <  SRR4302542.5236096/94‑1 (MQ=60)

                                                             CCACAACAGATGGGTATAAAGCACCCCGAGGCTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATA                                 <  SRR4302542.6316974/94‑1 (MQ=60)

                                                             CCACAACAGATGGGTATAAAGCACCCCGAGGCTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATA                                 <  SRR4302542.7011793/94‑1 (MQ=60)

                                                              CACAACAGATGGGTATAAAGCACCCCGAGGCTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAG                                <  SRR4302542.308026/94‑1 (MQ=60)

                                                              CACAACAGATGGGTATAAAGCACCCCGAGGCTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAG                                <  SRR4302542.2480866/94‑1 (MQ=60)

                                                               ACAACAGATGGGTATAAAGCACCCCGAGGCTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGC                               >  SRR4302542.4590586/1‑94 (MQ=60)

                                                               ACAACAGATGGGTATAAAGCACCCCGAGGCTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGC                               >  SRR4302542.7401299/1‑94 (MQ=60)

                                                                  ACAGATGGGTATAAAGCCCCCCGAGGCTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGT                            <  SRR4302542.3922330/94‑1 (MQ=60)

                                                                  ACAGATGGGTATAAAGCACCCCGAGGCTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGT                            <  SRR4302542.3388718/94‑1 (MQ=60)

                                                                   CAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTT                           >  SRR4302542.4640747/1‑94 (MQ=60)

                                                                    AGATGGGTATAAAGCACCCCGAGGCTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTT                          >  SRR4302542.896303/1‑94 (MQ=60)

                                                                    AGATGGGTATAAAGCACCCCGAGGCTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTT                          >  SRR4302542.1611820/1‑94 (MQ=60)

                                                                      ATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCC                        <  SRR4302542.5624847/94‑1 (MQ=60)

                                                                      ATGGGTATAAAGCACCCCGAGGCTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCC                        <  SRR4302542.3880357/94‑1 (MQ=60)

                                                                       TGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCA                       >  SRR4302542.2935355/1‑94 (MQ=60)

                                                                        GGGTATAAAGCACCCCGAGGCTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAG                      <  SRR4302542.1024823/94‑1 (MQ=60)

                                                                         GGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGT                     <  SRR4302542.2709402/94‑1 (MQ=60)

                                                                         GGTATAAAGCACCCCGAGGCTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGT                     <  SRR4302542.4619677/94‑1 (MQ=60)

                                                                          GTATAAAGCACCCCGAGGCTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTT                    >  SRR4302542.2717531/1‑94 (MQ=60)

                                                                          GTATAAAGCACCCCGAGGCTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTT                    <  SRR4302542.7049720/94‑1 (MQ=60)

                                                                            ATAAAGGACCCCGAGGCTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCA                  >  SRR4302542.4749590/1‑94 (MQ=60)

                                                                            ATAAAGCACCCCGAGGCTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCA                  >  SRR4302542.397914/1‑94 (MQ=60)

                                                                            ATAAAGCACCCCGAGGCTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCA                  >  SRR4302542.4594551/1‑94 (MQ=60)

                                                                              AAAGCACCCCGAGGTTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTG‑AGGGATCGATAGCCGTTTCCAGTTCATGG               <  SRR4302542.3494903/94‑1 (MQ=60)

                                                                              AAAGCACCCCGAGGCTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATG                <  SRR4302542.1021040/94‑1 (MQ=60)

                                                                              AAAGCACCCCGAGGCTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATG                <  SRR4302542.3486748/94‑1 (MQ=60)

                                                                              AAAGCACCCCGAGGCTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATG                >  SRR4302542.660474/1‑94 (MQ=60)

                                                                              AAAGCACCCCGAGGCTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATG                >  SRR4302542.3583301/1‑94 (MQ=60)

                                                                               AAGCACCCCGAGGCTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGG               <  SRR4302542.6531929/94‑1 (MQ=60)

                                                                                AGCACCCCGAGGCTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGC              <  SRR4302542.565975/94‑1 (MQ=60)

                                                                                   ACCCCGAGGCTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGAT           >  SRR4302542.2312378/1‑94 (MQ=60)

                                                                                     CCCGAGGTTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATAT         >  SRR4302542.3819446/1‑94 (MQ=60)

                                                                                     CCCGAGGCTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATAT         >  SRR4302542.7036712/1‑94 (MQ=60)

                                                                                     CCCGAGGCTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATAT         >  SRR4302542.2867821/1‑94 (MQ=60)

                                                                                     CCCGAGGCTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATAT         <  SRR4302542.776699/94‑1 (MQ=60)

                                                                                       CGAGGTTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTC       <  SRR4302542.5794381/94‑1 (MQ=60)

                                                                                          GGTTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACT    <  SRR4302542.3904398/94‑1 (MQ=60)

                                                                                          GGCTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCATTTCCAGTTCATGGCGATATTCACT    >  SRR4302542.5819519/1‑94 (MQ=60)

                                                                                           GCTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTT   >  SRR4302542.1189419/1‑94 (MQ=60)

                                                                                            CTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTT  <  SRR4302542.6413018/94‑1 (MQ=60)

                                                                                            CTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTT  >  SRR4302542.6305716/1‑94 (MQ=60)

GCGCCAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTT  >  NC_000913/3477305‑3477490

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`