Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1061 294.1 15065038 98.6% 14854127 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,509,198 A→G V189A (GTG→GCG)  yhfZ ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,509,1980AG81.3% 546.8 / 126.6 268V189A (GTG→GCG) yhfZhypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (25/25);  new base G (110/108);  total (135/133)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.85e-01

Only 100 of 272 total aligned reads displayed.

CTACCCGTTGTTGATGGGCGAGCAGGGCGTGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCACGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCCTCTAATCCCAGCATGGTCAGTTCGTTTTCCGCCACCACGTTC  >  NC_000913/3509106‑3509290
                                                                                            |                                                                                            
   cccGTTGTTGATGGGCGAGCAGGGCGTGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCgg                                                                                           <  2:5072024/93‑1 (MQ=255)
   cccGTTGTTGATGGGCGAGCAGGGCGTGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCACgg                                                                                           <  2:6572332/93‑1 (MQ=255)
     cGTTGTTGATGGGCGAGCAGGGCGTGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCt                                                                                         >  1:4320424/1‑93 (MQ=255)
     cGTTGTTGATGGGCGAGCAGGGCGTGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCt                                                                                         <  1:3580361/93‑1 (MQ=255)
     cGTTGTTGATGGGCGAGCAGGGCGTGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCt                                                                                         >  2:4144568/1‑93 (MQ=255)
      gttgttGATGGGCGAGCAGGGCGTGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCtt                                                                                        <  2:3212305/93‑1 (MQ=255)
      gttgttGATGGGCGAGCAGGGCGTGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCtt                                                                                        <  1:3130828/93‑1 (MQ=255)
       ttgttgATGGGCGAGCAGGGCGTGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCACGGCTTc                                                                                       <  2:4789669/93‑1 (MQ=255)
        tgttgATGGGCGAGCAGGGCGTGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTCg                                                                                      <  1:7071203/93‑1 (MQ=255)
        tgttgATGGGCGAGCAGGGCGTGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTCg                                                                                      <  2:7104857/93‑1 (MQ=255)
          ttgATGGGCGAGCAGGGCGTGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACGGCGGCTTCGGt                                                                                    <  1:551213/93‑1 (MQ=255)
           tgATGGGCGAGCAGGGCGTGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTg                                                                                   <  2:2258028/93‑1 (MQ=255)
              tGGGCGAGCAGGGCGTGTTTATCTACAACGGCACGCAGCAGTCGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGcc                                                                                <  1:7379373/93‑1 (MQ=255)
               gggCGAGCAGGGCGTGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCt                                                                               <  1:915652/93‑1 (MQ=255)
               gggCGAGCAGGGCGTGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCt                                                                               <  1:5461446/93‑1 (MQ=255)
                ggCGAGCAGGGCGTGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTg                                                                              <  2:4930034/93‑1 (MQ=255)
                 gCGAGCAGGGCGTGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGt                                                                             <  1:2078058/93‑1 (MQ=255)
                   gAGCAGGGCGTGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTaa                                                                           >  1:3262454/1‑93 (MQ=255)
                     gCAGGGCGTGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTaaaa                                                                         >  1:1089716/1‑93 (MQ=255)
                     gCAGGGCGTGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCACGGCTTCGGTGGCCTGTaaaa                                                                         >  1:2296227/1‑93 (MQ=255)
                     gCAGGGCGTGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCACGGCTTCGGTGGCCTGTaaaa                                                                         >  1:500521/1‑93 (MQ=255)
                      cAGGGCGTGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTaaaaa                                                                        <  1:4041930/93‑1 (MQ=255)
                        gggCGTGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATc                                                                      >  1:4833132/1‑93 (MQ=255)
                         ggCGTGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATcg                                                                     <  1:4541000/93‑1 (MQ=255)
                          gCGTGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATcgc                                                                    <  2:1417642/93‑1 (MQ=255)
                          gCGTGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATcgc                                                                    >  2:5978183/1‑93 (MQ=255)
                          gCGTGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATcgc                                                                    <  1:1468266/93‑1 (MQ=255)
                          gCGTGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATcgc                                                                    >  2:2147136/1‑93 (MQ=255)
                           cGTGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATcgcg                                                                   >  2:5419682/1‑93 (MQ=255)
                             tgtTTATCTACAACGGCGCGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGa                                                                 <  2:2538119/93‑1 (MQ=255)
                             tgtTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGa                                                                 >  1:5719230/1‑93 (MQ=255)
                             tgtTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGa                                                                 <  2:3637180/93‑1 (MQ=255)
                              gtTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGAt                                                                <  2:4470607/93‑1 (MQ=255)
                              gtTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGAt                                                                <  2:7046238/93‑1 (MQ=255)
                               tttATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATc                                                               <  1:5295878/93‑1 (MQ=255)
                                 tATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGt                                                             >  1:846447/1‑93 (MQ=255)
                                 tATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGt                                                             >  2:5306104/1‑93 (MQ=255)
                                 tATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCACGGCTTCGGTGGCCTGTAAAAATCGCGGATCGt                                                             >  2:2725421/1‑93 (MQ=255)
                                  aTCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTc                                                            <  2:3247227/93‑1 (MQ=255)
                                  aTCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTc                                                            >  1:3221651/1‑93 (MQ=255)
                                   tCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCt                                                           >  2:5563421/1‑93 (MQ=255)
                                   tCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCACGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCt                                                           <  2:5997086/93‑1 (MQ=255)
                                     tACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCtgt                                                         >  2:2175368/1‑93 (MQ=255)
                                     tACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCtgt                                                         >  2:432130/1‑93 (MQ=255)
                                       cAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCACGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGa                                                       <  2:1328305/93‑1 (MQ=255)
                                        aaCGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAg                                                      >  2:4618395/1‑93 (MQ=255)
                                          cGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAgcg                                                    <  2:6814246/93‑1 (MQ=255)
                                          cGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCACGGCTTCGGTGGCCTGTAAAAATCGCGGGTCGTCTGTGAgcg                                                    >  1:6420123/1‑93 (MQ=255)
                                            gCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAgcggc                                                  >  2:7491475/1‑93 (MQ=255)
                                              aCGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGt                                                >  2:4375509/1‑93 (MQ=255)
                                               cccagcagTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTCCGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTc                                               <  1:6228711/91‑1 (MQ=255)
                                               cGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCACGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTc                                               <  1:5054486/93‑1 (MQ=255)
                                                gcagcaGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCg                                              >  1:4091133/1‑93 (MQ=255)
                                                  agcagTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGcc                                            >  1:6078049/1‑93 (MQ=255)
                                                  agcagTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGcc                                            >  2:5752024/1‑93 (MQ=255)
                                                   gcagTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCct                                           <  2:2802577/93‑1 (MQ=255)
                                                   gcagTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCACGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCct                                           >  2:6255342/1‑93 (MQ=255)
                                                     agTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCctct                                         >  1:303199/1‑93 (MQ=255)
                                                     agTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCctct                                         >  1:1672167/1‑93 (MQ=255)
                                                       ttgttgCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCCTCTaa                                       >  1:2795924/1‑93 (MQ=255)
                                                       ttgttgCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCCTCTaa                                       <  1:3958896/93‑1 (MQ=255)
                                                       ttgttgCATTGGGTAATCATCGACTCGCGTCAGGACCACGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCCTCTaa                                       <  1:1570482/93‑1 (MQ=255)
                                                        tgttgCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCCTCTAAt                                      <  2:3344626/93‑1 (MQ=255)
                                                        tgttgCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCCTCTAAt                                      >  2:6155170/1‑93 (MQ=255)
                                                         gttgCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCTCCTCCaaac                                     >  1:1940680/1‑91 (MQ=255)
                                                         gttgCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCCTCTAATc                                     <  2:773823/93‑1 (MQ=255)
                                                         gttgCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCCTCTAATc                                     >  2:7229769/1‑93 (MQ=255)
                                                          ttgCATTGGGTAATCATCGACTCGCGTCAGGACCACGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCCTCTAATcc                                    >  2:371091/1‑93 (MQ=255)
                                                             cATTGGGTAATCATCGACTCGCGTCAGGACCACGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCCTCTAATCCCAg                                 <  2:7001287/93‑1 (MQ=255)
                                                              aTTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCCTCTAATCCCAGc                                >  2:1551829/1‑93 (MQ=255)
                                                               ttGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCCTCTAATCCCAGCa                               >  1:1024948/1‑93 (MQ=255)
                                                               ttGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCCTCTAATCCCAGCa                               >  1:2532582/1‑93 (MQ=255)
                                                               ttGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCCTCTAATCCCAGCa                               >  1:5255316/1‑93 (MQ=255)
                                                               ttGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCCTCTAATCCCAGCa                               <  1:7324733/93‑1 (MQ=255)
                                                                tGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCCTCTAATCCCAGCAt                              <  1:7226425/93‑1 (MQ=255)
                                                                tGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCCTCTAATCCCAGCAt                              <  2:3885523/93‑1 (MQ=255)
                                                                  ggTAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCCTCTAATCCCAGCATgg                            <  2:6750109/93‑1 (MQ=255)
                                                                   gTAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCCTCTAATCCCAGCATGGt                           >  1:5987692/1‑93 (MQ=255)
                                                                    tAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCCTCTAATCCCAGCATGGTc                          <  1:4689030/93‑1 (MQ=255)
                                                                    tAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCCTCTAATCCCAGCATGGTc                          <  2:7399361/93‑1 (MQ=255)
                                                                      atcatcGACTCGCGTCAGGACCGCGGCTTCGGTGGGCTGTAAAAAAAGCGGGTCGGCTGTGGGGGGGGGCGCCTCTAATCCCAGCATGGGCAg                        >  1:4120101/1‑93 (MQ=255)
                                                                      atcatcGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCCTCTAATCCCAGCATGGTCAg                        >  1:1219293/1‑93 (MQ=255)
                                                                      atcatcGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCCTCTAATCCCAGCATGGTCAg                        >  1:2673813/1‑93 (MQ=255)
                                                                       tcatcGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCCTCTAATCCCAGCATGGTCAGt                       >  2:1197393/1‑93 (MQ=255)
                                                                       tcatcGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCCTCTAATCCCAGCATGGTCAGt                       >  2:2421471/1‑93 (MQ=255)
                                                                       tcatcGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCCTCTAATCCCAGCATGGTCAGt                       >  1:4950432/1‑93 (MQ=255)
                                                                        catcGACTCGCGTCAGGACCACGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCCTCTAATCCCAGCATGGTCAGtt                      <  1:4797438/93‑1 (MQ=255)
                                                                         atcGACTCGCGTCAGGACCACGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCCTCTAATCCCAGCATGGTCAGTTc                     >  2:1016777/1‑93 (MQ=255)
                                                                         atcGACTCGCGTCAGGACCACGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCCTCTAATCCCAGCATGGTCAGTTc                     <  1:6687384/93‑1 (MQ=255)
                                                                            gACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCCTCTAATCCCAGCATGGTCAGTTCGtt                  <  2:5728104/93‑1 (MQ=255)
                                                                             aCTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCCTCTAATCCCAGCATGGTCAGTTCGttt                 >  2:6690781/1‑93 (MQ=255)
                                                                             aCTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCCTCTAATCCCAGCATGGTCAGTTCGttt                 <  2:5291297/93‑1 (MQ=255)
                                                                             aCTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCCTCTAATCCCAGCATGGTCAGTTCGttt                 <  1:6742663/93‑1 (MQ=255)
                                                                              cTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCCTCTAATCCCAGCATGGTCAGTTCGtttt                >  1:3832497/1‑93 (MQ=255)
                                                                                   gTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCCTCTAATCCCAGCATGGTCAGTTCGTTTTCCGcc           <  1:3731339/93‑1 (MQ=255)
                                                                                     cAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCCTCTAATCCCAGCATGGTCAGTTCGTTTTCCGccac         >  1:5773505/1‑93 (MQ=255)
                                                                                       ggACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCCTCTAATCCCAGCATGGTCAGTTCGTTTTCCGccacca       >  2:879445/1‑93 (MQ=255)
                                                                                        gACCGCGGCTTCGGTTGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCCTCTAATCCCAGCATGGTCAGTTCGTTTTCCGCcaccac      <  1:6284891/93‑1 (MQ=255)
                                                                                         aCCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCCTCTAATCCCAGCATGGTCAGTTCGTTTTCCGCCACCACg     >  2:1787661/1‑93 (MQ=255)
                                                                                          ccACGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCCTCTAATCCCAGCATGGTCAGTTCGTTTTCCGCCACCACGt    <  2:2935805/93‑1 (MQ=255)
                                                                                            |                                                                                            
CTACCCGTTGTTGATGGGCGAGCAGGGCGTGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCACGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCCTCTAATCCCAGCATGGTCAGTTCGTTTTCCGCCACCACGTTC  >  NC_000913/3509106‑3509290

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ACTACCCGTTGTTGATGGGCGAGCAGGGCGTGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCACGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCCTCTAATCCCAGCATGGTCAGTTCGTTTTCCGCCACCACGTTC  >  NC_000913/3509105‑3509290
                                                                                             |                                                                                            
ACTACCCGTTGTTGATGGGCGAGCAGGGCGTGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTGATCATCGACTCGCGTCAGGACCG                                                                                              <  SRR4302542.6327787/94‑1 (MQ=60)
ACTACCCGTTGTTGATGGGCGAGCAGGGCGTGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCG                                                                                              <  SRR4302542.4547889/94‑1 (MQ=60)
 CTACCCGTTGTTGATGGGCGAGCAGGGCGTGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCAC                                                                                             <  SRR4302542.1700953/94‑1 (MQ=60)
  TACCCGTTGTTGATGGGCGAGCAGGGCGTGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCG                                                                                            <  SRR4302542.6328011/94‑1 (MQ=60)
  TACCCGTTGTTGATGGGCGAGCAGGGCGTGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCG                                                                                            <  SRR4302542.267443/94‑1 (MQ=60)
  TACCCGTTGTTGATGGGCGAGCAGGGCGTGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCACG                                                                                            <  SRR4302542.3916070/94‑1 (MQ=60)
    CCCGTTGTTGATGGGCGAGCAGGGCGTGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGC                                                                                          >  SRR4302542.5214458/1‑94 (MQ=60)
     CCGTTGTTGATGGGCGAGCAGGGCGTGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCT                                                                                         >  SRR4302542.4362901/1‑94 (MQ=60)
      CGTTGTTGATGGGCGAGCAGGGCGTGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTT                                                                                        <  SRR4302542.3613770/94‑1 (MQ=60)
       GTTGTTGATGGGCGAGCAGGGCGTGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTC                                                                                       >  SRR4302542.6353384/1‑94 (MQ=60)
       GTTGTTGATGGGCGAGCAGGGCGTGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTC                                                                                       <  SRR4302542.3160600/94‑1 (MQ=60)
         TGTTGATGGGCGAGCAGGGCGTGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTCGG                                                                                     <  SRR4302542.7140603/94‑1 (MQ=60)
         TGTTGATGGGCGAGCAGGGCGTGTTTATCTACAACGGCACGCAGCAGTCGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTCGG                                                                                     <  SRR4302542.3808386/94‑1 (MQ=60)
          GTTGATGGGCGAGCAGGGCGTGGTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCACGGCTTCGGT                                                                                    >  SRR4302542.5963955/1‑94 (MQ=60)
           TTGATGGGCGAGCAGGGCGTGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACGGCGGCTTCGGTG                                                                                   <  SRR4302542.555975/94‑1 (MQ=60)
           TTGATGGGCGAGCAGGGCGTGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTG                                                                                   <  SRR4302542.4139475/94‑1 (MQ=60)
           TTGATGGGCGAGCAGGGCGTGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTG                                                                                   <  SRR4302542.7082210/94‑1 (MQ=60)
               TGGGCGAGCAGGGCGTGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCT                                                                               <  SRR4302542.2897761/94‑1 (MQ=60)
               TGGGCGAGCAGGGCGTGTTTATCTACAACGGCACGCAGCAGTCGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCT                                                                               <  SRR4302542.7451627/94‑1 (MQ=60)
                GGGCGAGCAGGGCGTGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTG                                                                              <  SRR4302542.923549/94‑1 (MQ=60)
                GGGCGAGCAGGGCGTGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTG                                                                              <  SRR4302542.5514507/94‑1 (MQ=60)
                 GGCGAGCAGGGCGTGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGT                                                                             >  SRR4302542.5818020/1‑94 (MQ=60)
                  GCGAGCAGGGCGTGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTA                                                                            >  SRR4302542.1127798/1‑94 (MQ=60)
                  GCGAGCAGGGCGTGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTA                                                                            <  SRR4302542.2096009/94‑1 (MQ=60)
                   CGAGCAGGGCGTGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAA                                                                           >  SRR4302542.5528916/1‑94 (MQ=60)
                   CGAGCAGGGCGTGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAA                                                                           >  SRR4302542.3293263/1‑94 (MQ=60)
                    GAGCAGGGCGTGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAA                                                                          >  SRR4302542.4670058/1‑94 (MQ=60)
                     AGCAGGGCGTGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAA                                                                         >  SRR4302542.1099054/1‑94 (MQ=60)
                     AGCAGGGCGTGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCACGGCTTCGGTGGCCTGTAAAA                                                                         >  SRR4302542.504827/1‑94 (MQ=60)
                     AGCAGGGCGTGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCACGGCTTCGGTGGCCTGTAAAA                                                                         >  SRR4302542.2315958/1‑94 (MQ=60)
                       CAGGGCGTGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAAT                                                                       <  SRR4302542.4081810/94‑1 (MQ=60)
                        AGGGCGTGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATC                                                                      >  SRR4302542.3547693/1‑94 (MQ=60)
                        AGGGCGTGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATC                                                                      <  SRR4302542.4382517/94‑1 (MQ=60)
                        AGGGCGTGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATC                                                                      <  SRR4302542.5328484/94‑1 (MQ=60)
                        AGGGCGTGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATC                                                                      >  SRR4302542.6119220/1‑94 (MQ=60)
                        AGGGCGTGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATC                                                                      >  SRR4302542.4880217/1‑94 (MQ=60)
                         GGGCGTGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCG                                                                     >  SRR4302542.917456/1‑94 (MQ=60)
                          GGCGTGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGC                                                                    <  SRR4302542.4585454/94‑1 (MQ=60)
                           GCGTGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCG                                                                   <  SRR4302542.1480943/94‑1 (MQ=60)
                           GCGTGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCACGGCTTCGGTGGCCTGTAAAAATCGCG                                                                   <  SRR4302542.3115309/94‑1 (MQ=60)
                            CGTGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGG                                                                  >  SRR4302542.6275443/1‑94 (MQ=60)
                            CGTGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGG                                                                  >  SRR4302542.5250335/1‑94 (MQ=60)
                             GTGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGA                                                                 >  SRR4302542.5774741/1‑94 (MQ=60)
                             GTGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGA                                                                 >  SRR4302542.2618148/1‑94 (MQ=60)
                             GTGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGA                                                                 >  SRR4302542.6174382/1‑94 (MQ=60)
                             GTGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGA                                                                 >  SRR4302542.2014787/1‑94 (MQ=60)
                             GTGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGA                                                                 <  SRR4302542.1959222/94‑1 (MQ=60)
                             GTGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCACGGCTTCGGTGGCCTGTAAAAATCGCGGA                                                                 >  SRR4302542.4868408/1‑94 (MQ=60)
                               GTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCACGGCTTCGGTGGCCTGTAAAAATCGCGGATC                                                               >  SRR4302542.3632946/1‑94 (MQ=60)
                                TTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCG                                                              <  SRR4302542.5347342/94‑1 (MQ=60)
                                 TTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGT                                                             >  SRR4302542.1067795/1‑94 (MQ=60)
                                 TTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGT                                                             >  SRR4302542.6570021/1‑94 (MQ=60)
                                 TTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGT                                                             >  SRR4302542.853743/1‑94 (MQ=60)
                                  TATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTC                                                            <  SRR4302542.1628099/94‑1 (MQ=60)
                                  TATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTC                                                            >  SRR4302542.3252142/1‑94 (MQ=60)
                                  TATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTC                                                            >  SRR4302542.4471046/1‑94 (MQ=60)
                                   ATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCT                                                           >  SRR4302542.1669149/1‑94 (MQ=60)
                                   ATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCT                                                           >  SRR4302542.6085613/1‑94 (MQ=60)
                                   ATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATAGTCT                                                           <  SRR4302542.7572494/94‑1 (MQ=60)
                                   ATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCACGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCT                                                           >  SRR4302542.4885218/1‑94 (MQ=60)
                                     CTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGT                                                         <  SRR4302542.5528109/94‑1 (MQ=60)
                                      TACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTG                                                        <  SRR4302542.5072283/94‑1 (MQ=60)
                                      TACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTG                                                        <  SRR4302542.1285836/94‑1 (MQ=60)
                                      TACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCACGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTG                                                        <  SRR4302542.499091/94‑1 (MQ=60)
                                       ACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGA                                                       <  SRR4302542.4743537/94‑1 (MQ=60)
                                        CAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAG                                                      <  SRR4302542.7171479/94‑1 (MQ=60)
                                        CAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAG                                                      <  SRR4302542.6760890/94‑1 (MQ=60)
                                        CAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAG                                                      >  SRR4302542.2498412/1‑94 (MQ=60)
                                         AACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGC                                                     <  SRR4302542.956157/94‑1 (MQ=60)
                                          ACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCG                                                    <  SRR4302542.2868709/94‑1 (MQ=60)
                                          ACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCG                                                    <  SRR4302542.3487603/94‑1 (MQ=60)
                                          ACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCACGGCTTCGGTGGCCTGTAAAAATCGCGGGTCGTCTGTGAGCG                                                    >  SRR4302542.6483392/1‑94 (MQ=60)
                                          ACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCACGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCG                                                    >  SRR4302542.3171507/1‑94 (MQ=60)
                                            GGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGC                                                  <  SRR4302542.459735/94‑1 (MQ=60)
                                              CACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCACGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGT                                                >  SRR4302542.4100092/1‑94 (MQ=60)
                                                CGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCG                                              >  SRR4302542.4188115/1‑94 (MQ=60)
                                                CGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCG                                              >  SRR4302542.4131484/1‑94 (MQ=60)
                                                CGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCACGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCG                                              <  SRR4302542.5103568/94‑1 (MQ=60)
                                                CGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCACGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCG                                              >  SRR4302542.7152649/1‑94 (MQ=60)
                                                CCCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTCCGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCG                                              <  SRR4302542.6290042/94‑1 (MQ=60)
                                                 GCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGC                                             >  SRR4302542.6353324/1‑94 (MQ=60)
                                                  CAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCC                                            >  SRR4302542.6136907/1‑94 (MQ=60)
                                                     CAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCCTCT                                         >  SRR4302542.5013353/1‑94 (MQ=60)
                                                     CAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCCTCT                                         >  SRR4302542.1686624/1‑94 (MQ=60)
                                                     CAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCCTCT                                         >  SRR4302542.5316177/1‑94 (MQ=60)
                                                     CAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCCTCT                                         >  SRR4302542.305844/1‑94 (MQ=60)
                                                      AGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCCTCTA                                        >  SRR4302542.3923696/1‑94 (MQ=60)
                                                       GTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCCTCTAA                                       <  SRR4302542.3653918/94‑1 (MQ=60)
                                                       GTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCCTCTAA                                       >  SRR4302542.2822825/1‑94 (MQ=60)
                                                       GTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCACGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCCTCTAA                                       >  SRR4302542.5914210/1‑94 (MQ=60)
                                                        TTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCCTCTAAT                                      <  SRR4302542.3997985/94‑1 (MQ=60)
                                                        TTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCACGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCCTCTAAT                                      <  SRR4302542.1584046/94‑1 (MQ=60)
                                                         TGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCtcctccaaac                                     >  SRR4302542.1957460/1‑84 (MQ=60)
                                                            TGCATTGGGTAATCATCGACTCGCGTCAGGACCACGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCCTCTAATCCCA                                  >  SRR4302542.4111238/1‑94 (MQ=60)
                                                              CATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCCTCTAATCCCAGC                                >  SRR4302542.5751228/1‑94 (MQ=60)
                                                               ATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCCTCTAATCCCAGCA                               >  SRR4302542.5306397/1‑94 (MQ=60)
                                                               ATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCCTCTAATCCCAGCA                               >  SRR4302542.2557150/1‑94 (MQ=60)
                                                               ATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCCTCTAATCCCAGCA                               >  SRR4302542.1033779/1‑94 (MQ=60)
                                                                TTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCCTCTAATCCCAGCAT                              <  SRR4302542.7396479/94‑1 (MQ=60)
                                                                 TGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCCTCTAATCCCAGCATG                             <  SRR4302542.7297311/94‑1 (MQ=60)
                                                                   GGTAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCCTCTAATCCCAGCATGGT                           >  SRR4302542.6045694/1‑94 (MQ=60)
                                                                     TAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCCTCTAATCCCAGCATGGTCA                         <  SRR4302542.4734825/94‑1 (MQ=60)
                                                                      AATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGGCTGTAAAAAAAGCGGGTCGGCTGTGGGGGGGGGCGCCTCTAATCCCAGCATGGGCAG                        >  SRR4302542.4160694/1‑94 (MQ=34)
                                                                      AATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCCTCTAATCCCAGCATGGTCAG                        >  SRR4302542.2699618/1‑94 (MQ=60)
                                                                      AATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCCTCTAATCCCAGCATGGTCAG                        <  SRR4302542.2717281/94‑1 (MQ=60)
                                                                      AATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCCTCTAATCCCAGCATGGTCAG                        <  SRR4302542.1451134/94‑1 (MQ=60)
                                                                      AATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCCTCTAATCCCAGCATGGTCAG                        >  SRR4302542.1229787/1‑94 (MQ=60)
                                                                       ATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCCTCTAATCCCAGCATGGTCAGT                       >  SRR4302542.1898679/1‑94 (MQ=60)
                                                                       ATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCCTCTAATCCCAGCATGGTCAGT                       >  SRR4302542.4998525/1‑94 (MQ=60)
                                                                        TCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCCTCTAATCCCAGCATGGTCAGTT                      <  SRR4302542.968393/94‑1 (MQ=60)
                                                                        TCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCCTCTAATCCCAGCATGGTCAGTT                      <  SRR4302542.5087277/94‑1 (MQ=60)
                                                                         CATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCCTCTAATCCCAGCATGGTCAGTTC                     <  SRR4302542.7394750/94‑1 (MQ=60)
                                                                         CATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCCTCTAATCCCAGCATGGTCAGTTC                     <  SRR4302542.6620521/94‑1 (MQ=60)
                                                                         CATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCCTCTAATCCCAGCATGGTCAGTTC                     >  SRR4302542.3293150/1‑94 (MQ=60)
                                                                         CATCGACTCGCGTCAGGACCACGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCCTCTAATCCCAGCATGGTCAGTTC                     <  SRR4302542.4844177/94‑1 (MQ=60)
                                                                          ATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCCTCTAATCCCAGCATGGTCAGTTCG                    <  SRR4302542.4292684/94‑1 (MQ=60)
                                                                          ATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCCTCTAATCCCAGCATGGTCAGTTCG                    <  SRR4302542.2316471/94‑1 (MQ=60)
                                                                          ATCGACTCGCGTCAGGACCACGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCCTCTAATCCCAGCATGGTCAGTTCG                    >  SRR4302542.4030606/1‑94 (MQ=60)
                                                                          ATCGACTCGCGTCAGGACCACGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCCTCTAATCCCAGCATGGTCAGTTCG                    <  SRR4302542.6753253/94‑1 (MQ=60)
                                                                            CGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCCTCTAATCCCAGCATGGTCAGTTCGTT                  <  SRR4302542.2558562/94‑1 (MQ=60)
                                                                            CGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCCTCTAATCCCAGCATGGTCAGTTCGTT                  >  SRR4302542.7306689/1‑94 (MQ=60)
                                                                             GACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCCTCTAATCCCAGCATGGTCAGTTCGTTT                 <  SRR4302542.5432830/94‑1 (MQ=60)
                                                                              ACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCCTCTAATCCCAGCATGGTCAGTTCGTTTT                <  SRR4302542.6809016/94‑1 (MQ=60)
                                                                              ACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCCTCTAATCCCAGCATGGTCAGTTCGTTTT                >  SRR4302542.3870401/1‑94 (MQ=60)
                                                                              ACTCGCGTCAGGACCACGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCCTCTAATCCCAGCATGGTCAGTTCGTTTT                >  SRR4302542.1146918/1‑94 (MQ=60)
                                                                               CTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCCTCTAATCCCAGCATGGTCAGTTCGTTTTC               >  SRR4302542.6312504/1‑94 (MQ=60)
                                                                                TCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCCTCTAATCCCAGCATGGTCAGTTCGTTTTCC              <  SRR4302542.5571625/94‑1 (MQ=60)
                                                                                 CGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCCTCTAATCCCAGCATGGTCAGTTCGTTTTCCG             <  SRR4302542.835489/94‑1 (MQ=60)
                                                                                 CGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCCTCTAATCCCAGCATGGTCAGTTCGTTTTCCG             >  SRR4302542.4845413/1‑94 (MQ=60)
                                                                                  GCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCCTCTAATCCCAGCATGGTCAGTTCGTTTTCCGC            >  SRR4302542.3098544/1‑94 (MQ=60)
                                                                                    GTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCCTCTAATCCCAGCATGGTCAGTTCGTTTTCCGCCA          <  SRR4302542.3768294/94‑1 (MQ=60)
                                                                                     TCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCCTCTAATCCCAGCATGGTCAGTTCGTTTTCCGCCAC         >  SRR4302542.5829548/1‑94 (MQ=60)
                                                                                     TCAGGACCACGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCCTCTAATCCCAGCATGGTCAGTTCGTTTTCCGCCAC         <  SRR4302542.3251547/94‑1 (MQ=60)
                                                                                     TCAGGACCACGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCCTCTAATCCCAGCATGGTCAGTTCGTTTTCCGCCAC         <  SRR4302542.1571469/94‑1 (MQ=60)
                                                                                      CAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCCTCTAATCCCAGCATGGTCAGTTCGTTTTCCGCCACC        <  SRR4302542.228302/94‑1 (MQ=60)
                                                                                        GGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCCTCTAATCCCAGCATGGTCAGTTCGTTTTCCGCCACCAC      <  SRR4302542.7071536/94‑1 (MQ=60)
                                                                                        GGACCACGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCCTCTAATCCCAGCATGGTCAGTTCGTTTTCCGCCACCAC      <  SRR4302542.3998989/94‑1 (MQ=60)
                                                                                         GACCGCGGCTTCGGTTGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCCTCTAATCCCAGCATGGTCAGTTCGTTTTCCGCCACCACG     <  SRR4302542.6346822/94‑1 (MQ=60)
                                                                                         GACCACGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCCTCTAATCCCAGCATGGTCAGTTCGTTTTCCGCCACCACG     <  SRR4302542.7430941/94‑1 (MQ=60)
                                                                                            CACGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCCTCTAATCCCAGCATGGTCAGTTCGTTTTCCGCCACCACGTTC  >  SRR4302542.916511/1‑94 (MQ=60)
                                                                                             |                                                                                            
ACTACCCGTTGTTGATGGGCGAGCAGGGCGTGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCACGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCCTCTAATCCCAGCATGGTCAGTTCGTTTTCCGCCACCACGTTC  >  NC_000913/3509105‑3509290

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑