Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1061 294.1 15065038 98.6% 14854127 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,714,436 2 bp→GC coding (261‑262/660 nt) yiaD → putative outer membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,714,4360CG99.7% 1018.4 / ‑6.5 307N87K (AAC→AAG) yiaDputative outer membrane protein
Reads supporting (aligned to +/- strand):  ref base C (0/1);  new base G (110/196);  total (110/197)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01
*NC_0009133,714,4370GC100.0% 1026.8 / NA 307V88L (GTG→CTG) yiaDputative outer membrane protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (110/197);  total (110/197)

Only 100 of 316 total aligned reads displayed.

TCGCGGTAAAGGCGCGCTGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAACGTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACAATGTGACCTTC  >  NC_000913/3714349‑3714523
                                                                                       ||                                                                                      
tCGCGGTAAAGGCGCGCTGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTgcg                                                                                    <  2:3925442/93‑1 (MQ=255)
tCGCGGTAAAGGCGCGCTGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTgcg                                                                                    >  1:6892373/1‑93 (MQ=255)
  gcgGTAAAGGCGCGCTGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGcgcg                                                                                  >  2:5479775/1‑93 (MQ=255)
    ggTAAAGGCGCGCTGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGAc                                                                                >  2:1727444/1‑93 (MQ=255)
     gTAAAGGCGCGCTGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACa                                                                               >  2:1101912/1‑93 (MQ=255)
      tAAAGGCGCGCTGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACaa                                                                              >  2:3258589/1‑93 (MQ=255)
        aaGGCGCGCTGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACaaaa                                                                            >  1:5148419/1‑93 (MQ=255)
        aaGGCGCGCTGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACaaaa                                                                            <  1:6584211/93‑1 (MQ=255)
         aGGCGCGCTGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCNGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGTGCGACAAAAt                                                                           <  2:4991265/93‑1 (MQ=255)
          ggCGCGCTGAGTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATg                                                                          <  1:289572/93‑1 (MQ=255)
            cgcgcTGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATgcg                                                                        <  1:7344868/93‑1 (MQ=255)
              cgcTGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGcgcg                                                                      <  1:83567/93‑1 (MQ=255)
               gcTGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCgg                                                                     >  1:3019565/1‑93 (MQ=255)
               gcTGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCgg                                                                     >  1:3103797/1‑93 (MQ=255)
               gcTGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCgg                                                                     >  1:5894265/1‑93 (MQ=255)
                cTGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGc                                                                    >  2:6837812/1‑93 (MQ=255)
                 tGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCa                                                                   >  1:1458625/1‑93 (MQ=255)
                 tGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCa                                                                   <  2:2295525/93‑1 (MQ=255)
                   aTTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGGGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACt                                                                 >  2:2931460/1‑93 (MQ=255)
                    ttGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTg                                                                >  2:3627204/1‑93 (MQ=255)
                    ttGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTg                                                                <  1:7221481/93‑1 (MQ=255)
                    ttGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTg                                                                <  1:4678112/93‑1 (MQ=255)
                     tGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTgg                                                               <  2:718003/93‑1 (MQ=255)
                      ggCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGgt                                                              >  2:4308593/1‑93 (MQ=255)
                      ggCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGgt                                                              <  1:2440957/93‑1 (MQ=255)
                          cagcagcagGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTa                                                          <  2:3767368/93‑1 (MQ=255)
                          cagcagcagGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTa                                                          <  2:6331252/93‑1 (MQ=255)
                           agcagcagGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAg                                                         <  2:4095623/93‑1 (MQ=255)
                            gcagcagGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGc                                                        >  2:4161249/1‑93 (MQ=255)
                               gcagGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTa                                                     >  1:3001094/1‑93 (MQ=255)
                               gcagGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTa                                                     >  2:5658801/1‑93 (MQ=255)
                               gcagGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTa                                                     >  1:6172071/1‑93 (MQ=255)
                                  ggCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAAcc                                                  >  2:5142348/1‑93 (MQ=255)
                                   gcgcAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAAccc                                                 <  1:2403663/93‑1 (MQ=255)
                                     gcAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGc                                               >  2:905536/1‑93 (MQ=255)
                                      cAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCa                                              <  1:3338187/93‑1 (MQ=255)
                                      cAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCa                                              <  1:4400532/93‑1 (MQ=255)
                                       aGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAg                                             <  2:731713/93‑1 (MQ=255)
                                       aGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAg                                             <  2:5381955/93‑1 (MQ=255)
                                       aGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAg                                             <  2:4953633/93‑1 (MQ=255)
                                        gCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGc                                            <  1:6334472/93‑1 (MQ=255)
                                        gCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGc                                            >  1:5380661/1‑93 (MQ=255)
                                        gCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGc                                            >  1:4499745/1‑93 (MQ=255)
                                         ctctGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCg                                           <  1:5525818/93‑1 (MQ=255)
                                          tctGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCgg                                          <  1:6003233/93‑1 (MQ=255)
                                          tctGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCgg                                          <  1:3246149/93‑1 (MQ=255)
                                          tctGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCgg                                          >  2:4792875/1‑93 (MQ=255)
                                            tGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCgggg                                        <  1:6689717/93‑1 (MQ=255)
                                             gggCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGa                                       <  1:7052459/93‑1 (MQ=255)
                                             gggCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGa                                       <  1:5663463/93‑1 (MQ=255)
                                             gggCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGa                                       <  1:3699717/93‑1 (MQ=255)
                                              ggcggcggcGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGAt                                      <  2:2447382/93‑1 (MQ=255)
                                                cggcggcgTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATaa                                    <  2:7132856/93‑1 (MQ=255)
                                                cggcggcgTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATaa                                    <  1:868565/93‑1 (MQ=255)
                                                 ggcggcgTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAAc                                   <  2:3907468/93‑1 (MQ=255)
                                                  gcggcgTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACa                                  <  1:108549/93‑1 (MQ=255)
                                                  gcggcgTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACa                                  <  2:5906937/93‑1 (MQ=255)
                                                     gcgTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTa                               <  2:2688701/93‑1 (MQ=255)
                                                     gcgTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTa                               >  2:4430148/1‑93 (MQ=255)
                                                       gTTGGTTATTACATGGAGGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATc                             <  1:2565702/93‑1 (MQ=255)
                                                        ttGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATcc                            <  1:4001616/93‑1 (MQ=255)
                                                        ttGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATcc                            >  2:4883699/1‑93 (MQ=255)
                                                         tGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCt                           <  2:2531565/93‑1 (MQ=255)
                                                          ggTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTc                          <  2:603821/93‑1 (MQ=255)
                                                           gTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCa                         >  2:5303764/1‑93 (MQ=255)
                                                           gTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCa                         <  1:4276092/93‑1 (MQ=255)
                                                            ttattaCATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCaa                        <  1:1859405/93‑1 (MQ=255)
                                                            ttattaCATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCaa                        <  1:5095991/93‑1 (MQ=255)
                                                            ttattaCATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCaa                        <  1:2819351/93‑1 (MQ=255)
                                                              attaCATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAata                      >  2:5254171/1‑93 (MQ=255)
                                                              attaCATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAata                      <  2:798879/93‑1 (MQ=255)
                                                              attaCATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAata                      >  2:6453646/1‑93 (MQ=255)
                                                               ttaCATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAatat                     <  1:2220019/93‑1 (MQ=255)
                                                               ttaCATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAatat                     <  2:4366788/93‑1 (MQ=255)
                                                               ttaCATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAatat                     <  2:1512998/93‑1 (MQ=255)
                                                                 aCATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGc                   >  2:4643089/1‑93 (MQ=255)
                                                                 aCATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGc                   <  2:3437779/93‑1 (MQ=255)
                                                                   aTGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCg                 <  1:3760288/93‑1 (MQ=255)
                                                                    tGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGa                >  2:7455282/1‑93 (MQ=255)
                                                                      gATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAAc              <  2:5347438/93‑1 (MQ=255)
                                                                        tgtgCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACaa            <  1:798812/93‑1 (MQ=255)
                                                                        tgtgCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACaa            <  1:3915535/93‑1 (MQ=255)
                                                                         gtgCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACAAt           <  2:1377967/93‑1 (MQ=255)
                                                                         gtgCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACAAt           <  2:2177805/93‑1 (MQ=255)
                                                                          tgCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACAAtg          <  1:2013444/93‑1 (MQ=255)
                                                                          tgCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACAAtg          <  1:3784490/93‑1 (MQ=255)
                                                                           gCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACAAtgt         <  1:4836861/93‑1 (MQ=255)
                                                                           gCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACAAtgt         >  2:4820296/1‑93 (MQ=255)
                                                                            cAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACAAtgtg        <  1:193012/93‑1 (MQ=255)
                                                                             aGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACAATGTGa       <  2:2501512/93‑1 (MQ=255)
                                                                             aGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACAATGTGa       >  1:2588725/1‑93 (MQ=255)
                                                                             aGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACAATGTGa       <  2:4208943/93‑1 (MQ=255)
                                                                              ggAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACAATGTGAc      >  1:7307113/1‑93 (MQ=255)
                                                                              ggAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACAATGTGAc      <  2:1787524/93‑1 (MQ=255)
                                                                               gaagcgaagcTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACAATGTGAcc     >  2:1928750/1‑93 (MQ=255)
                                                                                aaGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACAATGTGACCt    <  2:6699839/93‑1 (MQ=255)
                                                                                aaGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACAATGTGACCt    <  2:2767816/93‑1 (MQ=255)
                                                                                 aGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACAATGTGACCtt   <  1:5841824/93‑1 (MQ=255)
                                                                                  gCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACAATGTGACCTTc  <  1:1546531/93‑1 (MQ=255)
                                                                                  gCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACAATGTGACCTTc  <  1:3397944/93‑1 (MQ=255)
                                                                                       ||                                                                                      
TCGCGGTAAAGGCGCGCTGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAACGTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACAATGTGACCTTC  >  NC_000913/3714349‑3714523

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GAAAGATCGCGGTAAAGGCGCGCTGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAACGTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACAATGTGACCTTCGACAG  >  NC_000913/3714343‑3714528
                                                                                             |                                                                                            
GAAAGATCGCGGTAAAGGCGCGCTGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAG                                                                                              >  SRR4302542.4810076/1‑94 (MQ=60)
GAAAGATCGCGGTAAAGGCGCGCTGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAG                                                                                              <  SRR4302542.4580267/94‑1 (MQ=60)
     ATCGCGGTAAAGGCGCGCTGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCG                                                                                         <  SRR4302542.5742363/94‑1 (MQ=60)
     ATCGCGGTAAAGGCGCGCTGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCG                                                                                         >  SRR4302542.6960058/1‑94 (MQ=60)
      TCGCGGTAAAGGCGCGCTGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGC                                                                                        <  SRR4302542.2483476/94‑1 (MQ=60)
       CGCGGTAAAGGCGCGCTGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCG                                                                                       >  SRR4302542.286197/1‑94 (MQ=60)
           GTAAAGGCGCGCTGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAA                                                                                   >  SRR4302542.7388566/1‑94 (MQ=60)
           GTAAAGGCGCGCTGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAA                                                                                   >  SRR4302542.6385068/1‑94 (MQ=60)
            TAAAGGCGCGCTGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAA                                                                                  <  SRR4302542.3041400/94‑1 (MQ=60)
             AAAGGCGCGCTGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAA                                                                                 >  SRR4302542.5198463/1‑94 (MQ=60)
             AAAGGCGCGCTGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAA                                                                                 >  SRR4302542.3122916/1‑94 (MQ=60)
              AAGGCGCGCTGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAAT                                                                                <  SRR4302542.6649080/94‑1 (MQ=60)
               AGGCGCGCTGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTTTTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATG                                                                               >  SRR4302542.2505894/1‑94 (MQ=60)
               AGGCGCGCTGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATG                                                                               <  SRR4302542.1968341/94‑1 (MQ=60)
               AGGCGCGCTGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATG                                                                               >  SRR4302542.6357378/1‑94 (MQ=60)
               AGGCGCGCTGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATG                                                                               <  SRR4302542.3372812/94‑1 (MQ=60)
               AGGCGCGCTGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATG                                                                               <  SRR4302542.5479324/94‑1 (MQ=60)
                GGCGCGCTGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGC                                                                              <  SRR4302542.220106/94‑1 (MQ=60)
                GGCGCGCTGAGTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGC                                                                              <  SRR4302542.292095/94‑1 (MQ=60)
                  CGCGCTGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGC                                                                            <  SRR4302542.7416802/94‑1 (MQ=60)
                   GCGCTGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCG                                                                           <  SRR4302542.2407623/94‑1 (MQ=60)
                   GCGCTGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCG                                                                           <  SRR4302542.1506738/94‑1 (MQ=60)
                    CGCTGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGG                                                                          <  SRR4302542.84266/94‑1 (MQ=60)
                    CGCTGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGG                                                                          >  SRR4302542.5172063/1‑94 (MQ=60)
                    CGCTGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGG                                                                          >  SRR4302542.5951385/1‑94 (MQ=60)
                    CGCTGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGG                                                                          >  SRR4302542.3133295/1‑94 (MQ=60)
                    CGCTGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGG                                                                          >  SRR4302542.3048408/1‑94 (MQ=60)
                     GCTGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGC                                                                         >  SRR4302542.3271320/1‑94 (MQ=60)
                      CTGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCA                                                                        >  SRR4302542.1471214/1‑94 (MQ=60)
                         ATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTG                                                                     >  SRR4302542.2092975/1‑94 (MQ=60)
                         ATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTG                                                                     <  SRR4302542.2613350/94‑1 (MQ=60)
                          TTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGG                                                                    <  SRR4302542.7292321/94‑1 (MQ=60)
                          TTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGG                                                                    <  SRR4302542.4723824/94‑1 (MQ=60)
                           TGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGT                                                                   >  SRR4302542.6042743/1‑94 (MQ=60)
                            GGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTG                                                                  <  SRR4302542.2464710/94‑1 (MQ=60)
                            GGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACCGGTG                                                                  <  SRR4302542.7039309/94‑1 (MQ=60)
                                CAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAG                                                              <  SRR4302542.3314142/94‑1 (MQ=60)
                                CAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAG                                                              <  SRR4302542.5712547/94‑1 (MQ=60)
                                CAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAACCTGCGCGACAAAATGCGCGGCACTGGTGTTAG                                                              <  SRR4302542.4534654/94‑1 (MQ=60)
                                 AGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGC                                                             >  SRR4302542.7421075/1‑94 (MQ=60)
                                 AGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGC                                                             >  SRR4302542.200807/1‑94 (MQ=60)
                                 AGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGAGCGGCACTGGTGTTAGC                                                             >  SRR4302542.3968357/1‑94 (MQ=60)
                                  GCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCG                                                            <  SRR4302542.6054557/94‑1 (MQ=60)
                                   CAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGT                                                           <  SRR4302542.3675323/94‑1 (MQ=60)
                                    AGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTA                                                          >  SRR4302542.6231889/1‑94 (MQ=60)
                                    AGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTA                                                          >  SRR4302542.3029797/1‑94 (MQ=60)
                                       AGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACC                                                       <  SRR4302542.7310293/94‑1 (MQ=60)
                                       AGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACC                                                       >  SRR4302542.1698983/1‑94 (MQ=60)
                                        GGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCC                                                      <  SRR4302542.387309/94‑1 (MQ=60)
                                        GGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCC                                                      <  SRR4302542.6936107/94‑1 (MQ=60)
                                         GCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCG                                                     <  SRR4302542.2427059/94‑1 (MQ=60)
                                          CGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGC                                                    >  SRR4302542.534889/1‑94 (MQ=60)
                                           GCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCA                                                   <  SRR4302542.7316287/94‑1 (MQ=60)
                                            CAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAG                                                  <  SRR4302542.4846954/94‑1 (MQ=60)
                                            CAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAG                                                  >  SRR4302542.3806244/1‑94 (MQ=60)
                                            CAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAG                                                  <  SRR4302542.4443743/94‑1 (MQ=60)
                                            CAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAG                                                  <  SRR4302542.4715504/94‑1 (MQ=60)
                                            CAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAG                                                  >  SRR4302542.5727364/1‑94 (MQ=60)
                                            CAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAG                                                  <  SRR4302542.4443375/94‑1 (MQ=60)
                                            CAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAG                                                  <  SRR4302542.3369603/94‑1 (MQ=60)
                                             AGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGC                                                 >  SRR4302542.5432929/1‑94 (MQ=60)
                                             AGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGC                                                 <  SRR4302542.3671283/94‑1 (MQ=60)
                                             AGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGC                                                 <  SRR4302542.7401346/94‑1 (MQ=60)
                                             AGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGC                                                 >  SRR4302542.4543876/1‑94 (MQ=60)
                                             AGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGC                                                 >  SRR4302542.2950248/1‑94 (MQ=60)
                                             AGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGC                                                 >  SRR4302542.841589/1‑94 (MQ=60)
                                              GCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCG                                                <  SRR4302542.6396884/94‑1 (MQ=60)
                                              GCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCG                                                <  SRR4302542.4501680/94‑1 (MQ=60)
                                              GCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCG                                                <  SRR4302542.7310842/94‑1 (MQ=60)
                                               CTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGG                                               <  SRR4302542.5579527/94‑1 (MQ=60)
                                               CTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGG                                               <  SRR4302542.2325141/94‑1 (MQ=60)
                                               CTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGG                                               >  SRR4302542.859567/1‑94 (MQ=60)
                                               CTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGG                                               >  SRR4302542.7162726/1‑94 (MQ=60)
                                                TCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGG                                              <  SRR4302542.3652715/94‑1 (MQ=60)
                                                TCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGG                                              <  SRR4302542.6061373/94‑1 (MQ=60)
                                                TCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGG                                              <  SRR4302542.3276827/94‑1 (MQ=60)
                                                 CTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGG                                             <  SRR4302542.5820684/94‑1 (MQ=60)
                                                  TGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGA                                            <  SRR4302542.6755617/94‑1 (MQ=60)
                                                   GGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGAT                                           <  SRR4302542.5164554/94‑1 (MQ=60)
                                                   GGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGAT                                           <  SRR4302542.5718474/94‑1 (MQ=60)
                                                   GGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGAT                                           <  SRR4302542.3736328/94‑1 (MQ=60)
                                                   GGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGAT                                           <  SRR4302542.2699447/94‑1 (MQ=60)
                                                   GGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGAT                                           <  SRR4302542.7121695/94‑1 (MQ=60)
                                                   GGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGAT                                           <  SRR4302542.798105/94‑1 (MQ=60)
                                                    GGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATA                                          <  SRR4302542.1921883/94‑1 (MQ=60)
                                                     GCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAA                                         <  SRR4302542.5943354/94‑1 (MQ=60)
                                                     GCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAA                                         <  SRR4302542.2714017/94‑1 (MQ=60)
                                                     GCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAA                                         <  SRR4302542.5479037/94‑1 (MQ=60)
                                                      CGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAAC                                        <  SRR4302542.589747/94‑1 (MQ=60)
                                                      CGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAAC                                        <  SRR4302542.876055/94‑1 (MQ=60)
                                                       GGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACA                                       >  SRR4302542.4333967/1‑94 (MQ=60)
                                                       GGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACA                                       <  SRR4302542.2935441/94‑1 (MQ=60)
                                                        GCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACAT                                      <  SRR4302542.462330/94‑1 (MQ=60)
                                                        GCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACAT                                      <  SRR4302542.109440/94‑1 (MQ=60)
                                                        GCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACAT                                      <  SRR4302542.5528909/94‑1 (MQ=60)
                                                         CGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATT                                     <  SRR4302542.2448609/94‑1 (MQ=60)
                                                          GGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTA                                    >  SRR4302542.999777/1‑94 (MQ=60)
                                                           GCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAACGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTAT                                   <  SRR4302542.3763045/94‑1 (MQ=60)
                                                            CGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATC                                  >  SRR4302542.1096174/1‑94 (MQ=60)
                                                            CGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATC                                  <  SRR4302542.4037253/94‑1 (MQ=60)
                                                             GTTGGTTATTACATGGAGGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCC                                 <  SRR4302542.2590565/94‑1 (MQ=60)
                                                              TTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCT                                <  SRR4302542.4041081/94‑1 (MQ=60)
                                                              TTGGATATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCT                                >  SRR4302542.7046470/1‑94 (MQ=60)
                                                                GGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCA                              >  SRR4302542.6669348/1‑94 (MQ=60)
                                                                GGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCA                              <  SRR4302542.3375231/94‑1 (MQ=60)
                                                                 GTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAA                             <  SRR4302542.4318158/94‑1 (MQ=60)
                                                                  TTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAAT                            <  SRR4302542.5145515/94‑1 (MQ=60)
                                                                  TTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAAT                            <  SRR4302542.2846464/94‑1 (MQ=60)
                                                                  TTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAAT                            <  SRR4302542.6084058/94‑1 (MQ=60)
                                                                  TTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAAT                            <  SRR4302542.1875468/94‑1 (MQ=60)
                                                                   TATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATA                           <  SRR4302542.3110721/94‑1 (MQ=60)
                                                                   TATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATA                           >  SRR4302542.4174550/1‑94 (MQ=60)
                                                                    ATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATAT                          >  SRR4302542.2578099/1‑94 (MQ=60)
                                                                    ATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATAT                          <  SRR4302542.7255825/94‑1 (MQ=60)
                                                                    ATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATAT                          <  SRR4302542.807385/94‑1 (MQ=60)
                                                                     TTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATG                         >  SRR4302542.2434169/1‑94 (MQ=60)
                                                                     TTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATG                         <  SRR4302542.2272161/94‑1 (MQ=60)
                                                                     TTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATG                         <  SRR4302542.3771967/94‑1 (MQ=60)
                                                                     TTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATG                         <  SRR4302542.2239114/94‑1 (MQ=60)
                                                                      TACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGC                        >  SRR4302542.3045180/1‑94 (MQ=60)
                                                                       ACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCC                       >  SRR4302542.1482250/1‑94 (MQ=60)
                                                                        CATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCG                      <  SRR4302542.1843642/94‑1 (MQ=60)
                                                                         ATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGA                     <  SRR4302542.3874432/94‑1 (MQ=60)
                                                                         ATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGA                     <  SRR4302542.3797488/94‑1 (MQ=60)
                                                                         ATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGA                     <  SRR4302542.4859515/94‑1 (MQ=60)
                                                                            GATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACA                  <  SRR4302542.5067946/94‑1 (MQ=60)
                                                                             ATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACAA                 <  SRR4302542.6406069/94‑1 (MQ=60)
                                                                              TGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACAAT                <  SRR4302542.5024195/94‑1 (MQ=60)
                                                                              TGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACAAT                >  SRR4302542.1396527/1‑94 (MQ=60)
                                                                              TGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACAAT                <  SRR4302542.805679/94‑1 (MQ=60)
                                                                              TGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACAAT                <  SRR4302542.1926162/94‑1 (MQ=60)
                                                                              TGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACAAT                <  SRR4302542.3954172/94‑1 (MQ=60)
                                                                               GTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACAATG               <  SRR4302542.4405777/94‑1 (MQ=60)
                                                                               GTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACAATG               <  SRR4302542.690945/94‑1 (MQ=60)
                                                                                TGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACAATGT              >  SRR4302542.2848224/1‑94 (MQ=60)
                                                                                TGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACAATGT              <  SRR4302542.2030830/94‑1 (MQ=60)
                                                                                TGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACAATGT              <  SRR4302542.3821924/94‑1 (MQ=60)
                                                                                 GCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACAATGTG             <  SRR4302542.6291994/94‑1 (MQ=60)
                                                                                 GCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACAATGTG             <  SRR4302542.4883977/94‑1 (MQ=60)
                                                                                 GCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACAATGTG             <  SRR4302542.2594863/94‑1 (MQ=60)
                                                                                  CAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACAATGTGA            >  SRR4302542.930806/1‑94 (MQ=60)
                                                                                  CAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACAATGTGA            >  SRR4302542.3805345/1‑94 (MQ=60)
                                                                                  CAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACAATGTGA            <  SRR4302542.194647/94‑1 (MQ=60)
                                                                                  CAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACAATGTGA            >  SRR4302542.2613802/1‑94 (MQ=60)
                                                                                  CAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACAATGTGA            >  SRR4302542.4160822/1‑94 (MQ=60)
                                                                                   AGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACAATGTGAC           >  SRR4302542.2995898/1‑94 (MQ=60)
                                                                                   AGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACAATGTGAC           >  SRR4302542.7378691/1‑94 (MQ=60)
                                                                                    GGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACAATGTGACC          <  SRR4302542.5344566/94‑1 (MQ=60)
                                                                                     GAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACAATGTGACCT         <  SRR4302542.6406613/94‑1 (MQ=60)
                                                                                     GAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACAATGTGACCT         <  SRR4302542.6695568/94‑1 (MQ=60)
                                                                                      AAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACAATGTGACCTT        <  SRR4302542.5638870/94‑1 (MQ=60)
                                                                                      AAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACAATGTGACCTT        <  SRR4302542.844974/94‑1 (MQ=60)
                                                                                      AAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACAATGTGACCTT        <  SRR4302542.3282593/94‑1 (MQ=60)
                                                                                       AGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACAATGTGACCTTC       >  SRR4302542.2947711/1‑94 (MQ=60)
                                                                                       AGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACAATGTGACCTTC       <  SRR4302542.3242122/94‑1 (MQ=60)
                                                                                       AGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACAATGTGACCTTC       <  SRR4302542.6191595/94‑1 (MQ=60)
                                                                                       AGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACAATGTGACCTTC       >  SRR4302542.3283677/1‑94 (MQ=60)
                                                                                       AGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACAATGTGACCTTC       <  SRR4302542.5898490/94‑1 (MQ=60)
                                                                                        GCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACAATGTGACCTTCG      <  SRR4302542.1559876/94‑1 (MQ=60)
                                                                                        GCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACAATGTGACCTTCG      <  SRR4302542.3429819/94‑1 (MQ=60)
                                                                                            ACCTGCGCGACAAAATGCGCGGCACTGGTGTGCGCGCAACCCGCAGCGGGGATAACATTAGCCTCAATATGCCGAACAATGTGACCCTCGACCG  >  SRR4302542.2783446/1‑94 (MQ=60)
                                                                                             |                                                                                            
GAAAGATCGCGGTAAAGGCGCGCTGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAACGTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACAATGTGACCTTCGACAG  >  NC_000913/3714343‑3714528

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑