Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1061 294.1 15065038 98.6% 14854127 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,765,225 T→A D126E (GAT→GAA)  yibJ → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,765,2250TA85.8% 419.4 / 67.3 155D126E (GAT→GAA) yibJhypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (10/12);  new base A (55/78);  total (65/90)
Fisher's exact test for biased strand distribution p-value = 8.17e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.78e-01

Only 100 of 156 total aligned reads displayed.

CGCTATTATGACCCGCTGCAGGGGCGGTATATCACTCAGGATCCGATTGGGCTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGTAGATCCGCAAGGTTTGGTTGATATAAATTT  >  NC_000913/3765184‑3765317
                                         |                                                                                            
cGCTATTATGACCCGCTGCTGGGGCGGTATATCACTCAGGAACCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCg                                           <  1:6190330/93‑1 (MQ=11)
cGCTATTATGACCCGCTGCTGGGGCGGTATATCACTCAGGAACCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCg                                           <  2:3600433/93‑1 (MQ=255)
 gCTATTATGACCCGCTGCTGGGGCGGTATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCgg                                          <  1:6031325/93‑1 (MQ=12)
 gCTATTATGACCCGCTGCTGGGGCGGTATATCACTCAGGAACCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCgg                                          >  1:2279092/1‑93 (MQ=11)
  cTATTATGACCCGCTGCTGGGGCGGTATATCACTCAGGAACCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGt                                         >  1:7408718/1‑93 (MQ=11)
  cTATTATGACCCGCTGCTGGGGCGGTATATCACTCAGGAACCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGt                                         >  1:6053360/1‑93 (MQ=255)
  cTATTATGACCCGCTGCTGGGGCGGTATATCACTCAGGAACCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGt                                         <  1:1793720/93‑1 (MQ=255)
   tattatGACCCGCTGCTGGGGCGGTATATCACTCAGGAACCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTc                                        <  1:5373614/93‑1 (MQ=255)
   tattatGACCCGCTGCTGGGGCGGTATATCACTCAGGAACCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTc                                        >  1:1841865/1‑93 (MQ=11)
    attatGACCCGCTGCTGGGGCGGTATATCACTCAGGAACCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCa                                       <  2:2473433/93‑1 (MQ=255)
     ttatGACCCGCTGCTGGGGCGGTATATCACTCAGGAACCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCAt                                      >  2:6552153/1‑93 (MQ=11)
      tatGACCCGCTGCTGGGGCGGTATATCACTCAGGAACCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATa                                     >  2:6471833/1‑93 (MQ=14)
      tatGACCCGCTGCTGGGGCGGTATATCACTCAGGAACCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATa                                     >  1:7123895/1‑93 (MQ=255)
      tatGACCCGCTGCTGGGCCGGTATATCACTCAGGAACCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATa                                     >  2:4019234/1‑93 (MQ=14)
       atGACCCGCTGCTGGGGCGGTATATCACTCAGGAACCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATaa                                    >  1:2565487/1‑93 (MQ=255)
        tGACCCGCTGCTGGGGCGGTATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATaaa                                   >  2:7017575/1‑93 (MQ=255)
        tGACCCGCTGCTGGGGCGGTATATCACTCAGGAACCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATaaa                                   >  2:3999048/1‑93 (MQ=14)
        tGACCCGCTGCTGGGGCGGTATATCACTCAGGAACCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATaaa                                   <  2:7332181/93‑1 (MQ=255)
        tGACCCGCTGCTGGGGCGGTATATCACTCAGGAACCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATaaa                                   <  2:214392/93‑1 (MQ=255)
        tGACCCGCTGCTGGGGCGGTATATCACTCAGGAACCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATaaa                                   <  2:269937/93‑1 (MQ=255)
        tGACCCGCTGCTGGGGCGGTATATCACTCAGGAACCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATaaa                                   >  1:1388918/1‑93 (MQ=14)
        tGACCCGCTGCTGGGGCGGTATATCACTCAGGAACCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATaaa                                   <  2:5592055/93‑1 (MQ=255)
        tGACCCGCTGCTGGGGCGGTATATCACTCAGGAACCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATaaa                                   >  2:6810002/1‑93 (MQ=255)
         gACCCGCTGCTGGGGCGGTATATCACTCAGGAACCGATTGGACTGAAGGGGGGGTGGAATTTTTATCAGTATCCGTTGAATCTGGTCATCAAt                                  >  2:6672703/1‑93 (MQ=11)
         gACCCGCTGCTGGGGCGGTATATCACTCAGGAACCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAAt                                  <  2:5679361/93‑1 (MQ=255)
           cccGCTGCTGGGGCGGTATATCACTCAGGAACCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGt                                <  2:4420312/93‑1 (MQ=255)
           cccGCTGCTGGGGCGGTATATCACTCAGGAACCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGt                                <  2:5993345/93‑1 (MQ=255)
           cccGCTGCTGGGGCGGTATATCACTCAGGAACCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGt                                <  1:503658/93‑1 (MQ=255)
            ccGCTGCTGGGGCGGTATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGTa                               >  1:935215/1‑93 (MQ=255)
            ccGCTGCTGGGGCGGTATATCACTCAGGAACCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGTa                               <  1:5225013/93‑1 (MQ=255)
            ccGCTGCTGGGGCGGTATATCACTCAGGAACCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGTa                               <  1:7343578/93‑1 (MQ=255)
             cGCTGCTGGGGCGGTATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGTAg                              >  1:3105227/1‑93 (MQ=255)
             cGCTGCTGGGGCGGTATATCACTCAGGAACCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGTAg                              >  2:4101477/1‑93 (MQ=17)
             cGCTGCTGGGGCGGTATATCACTCAGGAACCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGTAg                              <  1:2975663/93‑1 (MQ=255)
             cGCTGCTGGGGCGGTATATCACTCAGGAACCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGTAg                              >  1:1283297/1‑93 (MQ=17)
              gctgctGGGGCGGTATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGTAGa                             >  2:6823404/1‑93 (MQ=255)
              gctgctGGGGCGGTATATCACTCAGGAACCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGTAGa                             <  1:6981792/93‑1 (MQ=255)
               ctgctgGGGCGGTATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGTAGAt                            >  1:3505997/1‑93 (MQ=255)
               ctgctgGGGCGGTATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGTAGAt                            <  1:6125868/93‑1 (MQ=255)
               ctgctgGGGCGGTATATCACTCAGGAACCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGTAGAt                            <  1:117759/93‑1 (MQ=255)
               ctgctgGGGCGGTATATCACTCAGGAACCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGTAGAt                            <  2:447573/93‑1 (MQ=255)
                tGCTGGGGCGGTATATCACTCAGGAACCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGTAGATc                           <  2:7413496/93‑1 (MQ=255)
                tGCTGGGGCGGTATATCACTCAGGAACCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGTAGATc                           <  2:3031367/93‑1 (MQ=255)
                tGCTGGGGCGGTATATCACTCAGGAACCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGTAGATc                           <  2:268446/93‑1 (MQ=255)
                tGCTGGGGCGGTATATCACTCAGGAACCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGTAGATc                           <  1:5170924/93‑1 (MQ=255)
                tGCTGGGGCGGTATATCACTCAGGAACCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGTAGATc                           >  2:2438878/1‑93 (MQ=17)
                tGCTGGGGCGGTATATCACTCAGGAACCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGTAGATc                           <  2:611210/93‑1 (MQ=255)
                  ctggggCGGTATATCACTCAGGAACCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGTAGATCCg                         <  2:2858003/91‑1 (MQ=255)
                   tggggCGGTATATCACTCAGGAACCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGTAGATCCGc                        >  1:1897124/2‑93 (MQ=255)
                    ggggCGGTATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGTAGATCCGCa                       >  1:4145236/1‑93 (MQ=255)
                    ggggCGGTATATCACTCAGGAACCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGTAGATCCGCa                       <  1:2839939/93‑1 (MQ=255)
                    ggggCGGTATATCACTCAGGAACCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGTAGATCCGCa                       >  2:3760681/1‑93 (MQ=255)
                     gggCGGTATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGTAGATCCGCaa                      >  2:4485671/1‑93 (MQ=255)
                     gggCGGTATATCACTCAGGAACCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGTAGATCCGCaa                      <  1:6102669/93‑1 (MQ=255)
                     gggCGGTATATCACTCAGGAACCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGTAGATCCGCaa                      >  2:4979699/1‑93 (MQ=255)
                      ggCGGTATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGTAGATCCGCAAg                     <  2:6346424/93‑1 (MQ=255)
                       gCGGTATATCACTCAGGAACCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGTAGATCCGCAAgg                    <  2:6295542/93‑1 (MQ=255)
                       gCGGTATATCACTCAGGAACCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGTAGATCCGCAAgg                    <  2:2296140/93‑1 (MQ=255)
                       gCGGTATATCACTCAGGAACCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGTAGATCCGCAAgg                    >  1:3171953/1‑93 (MQ=255)
                         ggTATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGTAGATCCGCAAGGtt                  <  1:4323997/93‑1 (MQ=255)
                         ggTATATCACTCAGGAACCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGTAGATCCGCAAGGtt                  >  1:3726873/1‑93 (MQ=255)
                          gTATATCACTCAGGAACCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGTAGATCCGCAAGGttt                 >  1:608481/1‑93 (MQ=255)
                          gTATATCACTCAGGAACCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGTAGATCCGCAAGGttt                 <  1:2072656/93‑1 (MQ=255)
                          gTATATCACTCAGGAACCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGTAGATCCGCAAGGttt                 >  1:1825375/1‑93 (MQ=255)
                          gTATATCACTCAGGAACCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGTAGATCCGCAAGGttt                 <  1:134816/93‑1 (MQ=255)
                          gTATATCACTCAGGAACCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGTAGATCCGCAAGGttt                 <  2:3769481/93‑1 (MQ=255)
                            atatCACTCAGGAACCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGTAGATCCGCAAGGTTTgg               <  2:2081231/93‑1 (MQ=255)
                            atatCACTCAGGAACCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGTAGATCCGCAAGGTTTgg               >  1:7024445/1‑93 (MQ=255)
                            atatCACTCAGGAACCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGTAGATCCGCAAGGTTTgg               <  2:4680053/93‑1 (MQ=255)
                            atatCACTCAGGAACCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGTAGATCCGCAAGGTTTgg               >  2:5219799/1‑93 (MQ=255)
                             tatCACTCAGGAACCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGTAGATCCGCAAGGTTTGGt              <  2:3867517/93‑1 (MQ=255)
                             tatCACTCAGGAACCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGTAGATCCGCAAGGTTTGGt              >  2:4756223/1‑93 (MQ=255)
                             tatCACTCAGGAACCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGTAGATCCGCAAGGTTTGGt              >  1:2554249/1‑93 (MQ=255)
                             tatCACTCAGGAACCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGTAGATCCGCAAGGTTTGGt              >  1:2476395/1‑93 (MQ=255)
                             tatCACTCAGGAACCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGTAGATCCGCAAGGTTTGGt              >  1:1461373/1‑93 (MQ=255)
                             tatCACTCAGGAACCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGTAGATCCGCAAGGTTTGGt              <  2:5937212/93‑1 (MQ=255)
                             tatCACTCAGGAACCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGTAGATCCGCAAGGTTTGGt              <  1:679143/93‑1 (MQ=255)
                             tatCACTCAGGAACCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGTAGATCCGCAAGGTTTGGt              >  1:4649515/1‑93 (MQ=255)
                              atCACTCAGGAACCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGTAGATCCGCAAGGTTTGGtt             <  2:2988380/93‑1 (MQ=255)
                               tCACTCAGGAACCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGTAGATCCGCAAGGTTTGGTTg            >  1:3110989/1‑93 (MQ=255)
                               tCACTCAGGAACCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGTAGATCCGCAAGGTTTGGTTg            >  2:2179458/1‑93 (MQ=255)
                               tCACTCAGGAACCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGTAGATCCGCAAGGTTTGGTTg            <  1:3038417/93‑1 (MQ=255)
                                cACTCAGGATCCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGTAGATCCGCAAGGTTTGGTTGa           <  1:3718897/93‑1 (MQ=255)
                                cACTCAGGAACCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGTAGATCCGCAAGGTTTGGTTGa           <  1:6711083/93‑1 (MQ=255)
                                 aCTCAGGATCCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGTAGATCCGCAAGGTTTGGTTGat          >  2:5401869/1‑93 (MQ=255)
                                  cTCAGGATCCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGTAGATCCGCAAGGTTTGGTTGata         <  1:3174974/93‑1 (MQ=255)
                                  cTCAGGAACCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGTAGATCCGCAAGGTTTGGTTGata         >  1:6351321/1‑93 (MQ=255)
                                  cTCAGGAACCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGTAGATCCGCAAGGTTTGGTTGata         <  2:4976357/93‑1 (MQ=255)
                                   tCAGGAACCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGTAGATCCGCAAGGTTTGGTTGatat        >  2:6180309/1‑93 (MQ=255)
                                   tCAGGAACCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGTAGATCCGCAAGGTTTGGTTGatat        <  2:1076605/93‑1 (MQ=255)
                                   tCAGGAACCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGTAGATCCGCAAGGTTTGGTTGatat        <  2:3622777/93‑1 (MQ=255)
                                   tCAGGAACCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGTAGATCCGCAAGGTTTGGTTGatat        >  1:100016/1‑93 (MQ=255)
                                    cAGGAACCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGTAGATCCGCAAGGTTTGGTTGAtata       <  1:161083/93‑1 (MQ=255)
                                    cAGGAACCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGTAGATCCGCAAGGTTTGGTTGAtata       <  2:3392529/93‑1 (MQ=255)
                                     aGGATCCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGTAGATCCGCAAGGTTTGGTTGATATaa      <  2:3510273/93‑1 (MQ=255)
                                     aGGAACCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGTAGATCCGCAAGGTTTGGTTGATATaa      >  1:565540/1‑93 (MQ=255)
                                     aGGAACCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGTAGATCCGCAAGGTTTGGTTGATATaa      <  1:1592049/93‑1 (MQ=255)
                                      ggATCCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGTAGATCCGCAAGGTTTGGTTGATATaaa     <  2:2523145/93‑1 (MQ=255)
                                      ggAACCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGTAGATCCGCAAGGTTTGGTTGATATaaa     <  2:5206352/93‑1 (MQ=255)
                                         tCCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGTAGATCCGCAAGGTTTGGTTGATATAAAttt  >  1:5698086/1‑93 (MQ=255)
                                         |                                                                                            
CGCTATTATGACCCGCTGCAGGGGCGGTATATCACTCAGGATCCGATTGGGCTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGTAGATCCGCAAGGTTTGGTTGATATAAATTT  >  NC_000913/3765184‑3765317

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCTGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGGTATATCACTCAGGATCCGATTGGGCTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGTAGATCCGCAAGGTTTGGTTGATATAAATTT  >  NC_000913/3765132‑3765317
                                                                                             |                                                                                            
CCTGCCGGGTCAGCAGTATGATGAGGAGTCCGGCCTGTATTACAACCGCCACCGCTATTATGACCCGCTGCTGGGGCGGTATATCACTCAGGAA                                                                                              >  SRR4302542.2752006/1‑94 (MQ=0)
CCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCTGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGGTATATCACTCAGGAT                                                                                              >  SRR4302542.4353673/1‑94 (MQ=0)
CCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCTGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGGTATATCACTCAGGAT                                                                                              <  SRR4302542.4634298/94‑1 (MQ=0)
 CTGCCGGGTCAGCAGTATGATGAGGAGTCCGGCCTGTATTACAACCGCCACCGCTATTATGACCCGCTGCTGGGGCGGTATATCACTCAGGATC                                                                                             >  SRR4302542.3790500/1‑94 (MQ=0)
 CTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCTGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGGTATATCACTCAGGATC                                                                                             <  SRR4302542.3874606/94‑1 (MQ=0)
 CTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCTGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGGTATATCACTCAGGATC                                                                                             >  SRR4302542.6699117/1‑94 (MQ=0)
  TGCCGGGTCAGCAGTATGATGAGGAGTCCGGCCTGTATTACAACCGCCACCGCTATTATGACCCGCTGCTGGGGCGGTATATCACTCAGGAACC                                                                                            <  SRR4302542.2658984/94‑1 (MQ=0)
  TGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCTGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGGTATATCACTCAGGATCC                                                                                            >  SRR4302542.6328968/1‑94 (MQ=0)
    CCGGGGCAGCAGTATGATGAGGAGTCCGGCCTGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGGTATATCACTCAGGATCCGA                                                                                          >  SRR4302542.1116423/1‑94 (MQ=0)
      GGGGCAGCAGTATGATGAGGAGTCCGGCCTGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGGTATATCACTCAGGATCCGATT                                                                                        <  SRR4302542.3658866/94‑1 (MQ=0)
       GGTCAGCAGTATGATGAGGAGTCCGGCCTGTATTACAACCGCCACCGCTATTATGACCCGCTGCTGGGGCGGTATATCACTCAGGAACCGATTG                                                                                       <  SRR4302542.1530335/94‑1 (MQ=0)
       GGTCAGCAGTATGATGAGGAGTCCGGCCTGTATTACAACCGCCACCGCTATTATGACCCGCTGCTGGGGCGGTATATCACTCAGGAACCGATTG                                                                                       <  SRR4302542.7268919/94‑1 (MQ=0)
         GCAGCAGTATGATGAGGAGTCCGGCCTGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGGTATATCACTCAGGATCCGATTGGG                                                                                     <  SRR4302542.3711908/94‑1 (MQ=0)
           AGCAGTATGATGAGGAGTCCGGCCTGTATTACAACCGCCACCGCTATTATGACCCGCTGCTGGGGCGGTATATCACTCAGGAACCGATTGGACT                                                                                   >  SRR4302542.5583061/1‑94 (MQ=0)
           AGCAGTATGATGAGGAGTCCGGCCTGTATTACAACCGCCACCGCTATTATGACCCGCTGCTGGGGCGGTATATCACTCAGGAACCGATTGGACT                                                                                   >  SRR4302542.643259/1‑94 (MQ=0)
           AGCAGTATGATGAGGAGTCCGGCCTGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGGTATATCACTCAGGATCCGATTGGGCT                                                                                   <  SRR4302542.2132169/94‑1 (MQ=0)
           AGCAGTATGATGAGGAGTCCGGCCTGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGGTATATCACTCAGGATCCGATTGGGCT                                                                                   <  SRR4302542.6314479/94‑1 (MQ=0)
           AGCAGTATGATGAGGAGTCCGGCCTGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGGTATATCACTCAGGATCCGATTGGGCT                                                                                   <  SRR4302542.6885589/94‑1 (MQ=0)
              AGTATGATGAGGAGTCCGGCCTGTATTACAACCGCCACCGCTATTATGACCCGCTGCTGGGGCGGTATATCACTCAGGAACCGATTGGACTGAA                                                                                <  SRR4302542.5531426/94‑1 (MQ=0)
               GTATGATGAGGAGTCCGGCCTGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGGTATATCACTCAGGATCCGATTGGGCTGAAG                                                                               >  SRR4302542.1688182/1‑94 (MQ=0)
               GTATGATGAGGAGTCCGGCCTGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGGTATATCACTCAGGATCCGATTGGGCTGAAG                                                                               <  SRR4302542.5574001/94‑1 (MQ=0)
               GTATGATGAGGAGTCCGGCCTGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGGTATATCACTCAGGATCCGATTGGGCTGAAG                                                                               >  SRR4302542.6957371/1‑94 (MQ=0)
                TATGATGAGGAGTCCGGCCTGTATTACAACCGCCACCGCTATTATGACCCGCTGCTGGGGCGGTATATCACTCAGGAACCGATTGGACTGAAGG                                                                              >  SRR4302542.6122635/1‑94 (MQ=0)
                 ATGATGAGGAGTCCGGCCTGTATTACAACCGCCACCGCTATTATGACCCGCTGCTGGGGCGGTATATCACTCAGGAACCGATTGGACTGAAGGG                                                                             <  SRR4302542.6987348/94‑1 (MQ=0)
                 ATGATGAGGAGTCCGGCCTGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGGTATATCACTCAGGATCCGATTGGGCTGAAGGG                                                                             >  SRR4302542.1829126/1‑94 (MQ=0)
                  TGATGAGGAGTCCGGCCTGTATTACAACCGCCACCGCTATTATGACCCGCTGCTGGGGCGGTATATCACTCAGGAACCGATTGGACTGAAGGGG                                                                            <  SRR4302542.6328595/94‑1 (MQ=0)
                  TGATGAGGAGTCCGGCCTGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGGTATATCACTCAGGATCCGATTGGGCTGAAGGGG                                                                            >  SRR4302542.2370981/1‑94 (MQ=0)
                  TGATGAGGAGTCCGGCCTGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGGTATATCACTCAGGATCCGATTGGGCTGAAGGGG                                                                            <  SRR4302542.4758425/94‑1 (MQ=0)
                   GATGAGGAGTCCGGCCTGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGGTATATCACTCAGGATCCGATTGGGCTGAAGGGGG                                                                           <  SRR4302542.7423191/94‑1 (MQ=0)
                    ATGAGGAGTCCGGCCTGTATTACAACCGCCACCGCTATTATGACCCGCTGCTGGGGCGGTATATCACTCAGGATCCGATTGGACTGAAGGGGGG                                                                          <  SRR4302542.5211500/94‑1 (MQ=0)
                    ATGAGGAGTCCGGCCTGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGGTATATCACTCAGGATCCGATTGGGCTGAAGGGGGG                                                                          <  SRR4302542.2895728/94‑1 (MQ=0)
                     TGAGGAGTCCGGCCTGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGGTATATCACTCAGGATCCGATTGGGCTGAAGGGGGGA                                                                         <  SRR4302542.4506408/94‑1 (MQ=0)
                      GAGGAGTCCGGCCTGTATTACAACCGCCACCGCTATTATGACCCGCTGCTGGGGCGGTATATCACTCAGGAACCGATTGGACTGAAGGGGGGAT                                                                        <  SRR4302542.3548956/94‑1 (MQ=0)
                      GAGGAGTCCGGCCTGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGGTATATCACTCAGGATCCGATTGGGCTGAAGGGGGGAT                                                                        <  SRR4302542.956414/94‑1 (MQ=0)
                       AGGAGTCCGGCCTGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGGTATATCACTCAGGATCCGATTGGGCTGAAGGGGGGATG                                                                       <  SRR4302542.4581230/94‑1 (MQ=0)
                        GGAGTCCGGCCTGTATTACAACCGCCACCGCTATTATGACCCGCTGCTGGGGCGGTATATCACTCAGGAACCGATTGGACTGAAGGGGGGATGG                                                                      <  SRR4302542.1764001/94‑1 (MQ=0)
                         GAGTCCGGCCTGTATTACAACCGCCACCGCTATTATGACCCGCTGCTGGGGCGGTATATCACTCAGGAACCGATTGGACTGAAGGGGGGATGGA                                                                     >  SRR4302542.1291882/1‑94 (MQ=0)
                          AGTCCGGCCTGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGGTATATCACTCAGGATCCGATTGGGCTGAAGGGGGGATGGAA                                                                    >  SRR4302542.7223392/1‑94 (MQ=0)
                           GTCCGGCCTGTATTACAACCGCCACCGCTATTATGACCCGCTGCTGGGGCGGTATATCACTCAGGAACCGATTGGACTGAAGGGGGGATGGAAT                                                                   >  SRR4302542.5220455/1‑94 (MQ=0)
                           GTCCGGCCTGTATTACAACCGCCACCGCTATTATGACCCGCTGCTGGGGCGGTATATCACTCAGGAACCGATTGGACTGAAGGGGGGATGGAAT                                                                   <  SRR4302542.6604698/94‑1 (MQ=0)
                           GTCCGGCCTGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGGTATATCACTCAGGATCCGATTGGGCTGAAGGGGGGATGGAAT                                                                   >  SRR4302542.4707900/1‑94 (MQ=0)
                           GTCCGGCCTGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGGTATATCACTCAGGATCCGATTGGGCTGAAGGGGGGATGGAAT                                                                   >  SRR4302542.1972250/1‑94 (MQ=0)
                            TCCGGCCTGTATTACAACCGCCACCGCTATTATGACCCGCTGCTGGGGCGGTATATCACTCAGGAACCGATTGGACTGAAGGGGGGATGGAATT                                                                  >  SRR4302542.4527606/1‑94 (MQ=0)
                             CCGGCCTGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGGTATATCACTCAGGATCCGATTGGGCTGAAGGGGGGATGGAATTT                                                                 >  SRR4302542.5611254/1‑94 (MQ=0)
                              CGGCCTGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGGTATATCACTCAGGATCCGATTGGGCTGAAGGGGGGATGGAATTTT                                                                >  SRR4302542.4439738/1‑94 (MQ=0)
                                GCCTGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGGTATATCACTCAGGATCCGATTGGGCTGAAGGGGGGATGGAATTTTTA                                                              >  SRR4302542.1866616/1‑94 (MQ=0)
                                 CCTGTATTACAACCGCCACCGCTATTATGACCCGCTGCTGGGGCGGTATATCACTCAGGAACCGATTGGACTGAAGGGGGGATGGAATTTTTAT                                                             <  SRR4302542.6835316/94‑1 (MQ=0)
                                 CCTGTATTACAACCGCCACCGCTATTATGACCCGCTGCTGGGGCGGTATATCACTCAGGAACCGATTGGACTGAAGGGGGGATGGAATTTTTAT                                                             >  SRR4302542.140781/1‑94 (MQ=0)
                                  CTGTATTACAACCGCCACCGCTATTATGACCCGCTGCTGGGGCGGTATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAATTTTTATC                                                            >  SRR4302542.505750/1‑94 (MQ=0)
                                  CTGTATTACAACCGCCACCGCTATTATGACCCGCTGCTGGGGCGGTATATCACTCAGGAACCGATTGGACTGAAGGGGGGATGGAATTTTTATC                                                            >  SRR4302542.2732024/1‑94 (MQ=0)
                                  CTGTATTACAACCGCCACCGCTATTATGACCCGCTGCTGGGGCGGTATATCACTCAGGAACCGATTGGACTGAAGGGGGGATGGAATTTTTATC                                                            >  SRR4302542.6565094/1‑94 (MQ=0)
                                  CTGTATTACAACCGCCACCGCTATTATGACCCGCTGCTGGGGCGGTATATCACTCAGGAACCGATTGGACTGAAGGGGGGATGGAATTTTTATC                                                            >  SRR4302542.3271527/1‑94 (MQ=0)
                                  CTGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGGTATATCACTCAGGATCCGATTGGGCTGAAGGGGGGATGGAATTTTTATC                                                            >  SRR4302542.716591/1‑94 (MQ=0)
                                  CTGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGGTATATCACTCAGGATCCGATTGGGCTGAAGGGGGGATGGAATTTTTATC                                                            >  SRR4302542.2540090/1‑94 (MQ=0)
                                   TGTATTACAACCGCCACCGCTATTATGACCCGCTGCTGGGGCGGTATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAATTTTTATCA                                                           <  SRR4302542.717213/94‑1 (MQ=0)
                                   TGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGGTATATCACTCAGGATCCGATTGGGCTGAAGGGGGGATGGAATTTTTATCA                                                           <  SRR4302542.2607940/94‑1 (MQ=0)
                                   TGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGGTATATCACTCAGGATCCGATTGGGCTGAAGGGGGGATGGAATTTTTATCA                                                           >  SRR4302542.2077868/1‑94 (MQ=0)
                                    GTATTACAACCGCCACCGCTATTATGACCCGCTGCTGGGGCGGTATATCACTCAGGAACCGATTGGACTGAAGGGGGGATGGAATTTTTATCAG                                                          <  SRR4302542.4262878/94‑1 (MQ=0)
                                    GTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGGTATATCACTCAGGATCCGATTGGGCTGAAGGGGGGATGGAATTTTTATCAG                                                          <  SRR4302542.974783/94‑1 (MQ=0)
                                    GTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGGTATATCACTCAGGATCCGATTGGGCTGAAGGGGGGATGGAATTTTTATCAG                                                          <  SRR4302542.3510428/94‑1 (MQ=0)
                                     TATTACAACCGCCACCGCTATTATGACCCGCTGCTGGGGCGGTATATCACTCAGGAACCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGT                                                         >  SRR4302542.719949/1‑94 (MQ=0)
                                     TATTACAACCGCCACCGCTATTATGACCCGCTGCTGGGGCGGTATATCACTCAGGAACCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGT                                                         >  SRR4302542.7020691/1‑94 (MQ=0)
                                      ATTACAACCGCCACCGCTATTATGACCCGCTGCTGGGGCGGTATATCACTCAGGAACCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTA                                                        >  SRR4302542.1199694/1‑94 (MQ=0)
                                      ATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGGTATATCACTCAGGATCCGATTGGGCTGAAGGGGGGATGGAATTTTTATCAGTA                                                        <  SRR4302542.5983784/94‑1 (MQ=0)
                                       TTACAACCGCCACCGCTATTATGACCCGCTGCTGGGGCGGTATATCACTCAGGAACCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTAT                                                       <  SRR4302542.5522689/94‑1 (MQ=0)
                                       TTACAACCGCCACCGCTATTATGACCCGCTGCTGGGGCGGTATATCACTCAGGAACCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTAT                                                       <  SRR4302542.5849857/94‑1 (MQ=0)
                                        TACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGGTATATCACTCAGGATCCGATTGGGCTGAAGGGGGGATGGAATTTTTATCAGTATC                                                      <  SRR4302542.98758/94‑1 (MQ=0)
                                         ACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGGTATATCACTCAGGATCCGATTGGGCTGAAGGGGGGATGGAATTTTTATCAGTATCC                                                     <  SRR4302542.6480833/94‑1 (MQ=0)
                                         ACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGGTATATCACTCAGGATCCGATTGGGCTGAAGGGGGGATGGAATTTTTATCAGTATCC                                                     >  SRR4302542.4994258/1‑94 (MQ=0)
                                          CAACCGCCACCGCTATTATGACCCGCTGCTGGGGCGGTATATCACTCAGGAACCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCG                                                    >  SRR4302542.5977861/1‑94 (MQ=0)
                                          CAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGGTATATCACTCAGGATCCGATTGGGCTGAAGGGGGGATGGAATTTTTATCAGTATCCG                                                    <  SRR4302542.776614/94‑1 (MQ=0)
                                           AACCGCCACCGCTATTATGACCCGCTGCTGGGGCGGTATATCACTCAGGAACCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGT                                                   >  SRR4302542.7411600/1‑94 (MQ=0)
                                            CCCGCCACCGCTATTATGACCCGCTGCTGGGGCGGTATATCACTCAGGAACCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTT                                                  <  SRR4302542.184433/94‑1 (MQ=0)
                                            ACCGCCACCGCTATTATGACCCGCTGCAGGGGCGGTATATCACTCAGGATCCGATTGGGCTGAAGGGGGGATGGAATTTTTATCAGTATCCGTT                                                  <  SRR4302542.2504676/94‑1 (MQ=0)
                                             CCGCCACCGCTATTATGACCCGCTGCTGGGGCGGTATATCACTCAGGAACCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTG                                                 <  SRR4302542.479490/94‑1 (MQ=0)
                                             CCGCCACCGCTATTATGACCCGCTGCAGGGGCGGTATATCACTCAGGATCCGATTGGGCTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTG                                                 >  SRR4302542.954615/1‑94 (MQ=0)
                                              CGCCACCGCTATTATGACCCGCTGCAGGGGCGGTATATCACTCAGGATCCGATTGGGCTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGA                                                <  SRR4302542.1321275/94‑1 (MQ=0)
                                                CCACCGCTATTATGACCCGCTGCAGGGGCGGTATATCACTCAGGATCCGATTGGGCTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAAT                                              <  SRR4302542.6685877/94‑1 (MQ=0)
                                                 CACCGCTATTATGACCCGCTGCTGGGGCGGTATATCACTCAGGAACCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATC                                             >  SRR4302542.7546429/1‑94 (MQ=0)
                                                 CACCGCTATTATGACCCGCTGCAGGGGCGGTATATCACTCAGGATCCGATTGGGCTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATC                                             <  SRR4302542.5509795/94‑1 (MQ=0)
                                                  ACCGCTATTATGACCCGCTGCAGGGGCGGTATATCACTCAGGATCCGATTGGGCTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCC                                            <  SRR4302542.5189989/94‑1 (MQ=0)
                                                  ACCGCTATTATGACCCGCTGCAGGGGCGGTATATCACTCAGGATCCGATTGGGCTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCC                                            >  SRR4302542.3374489/1‑94 (MQ=0)
                                                   CCGCTATTATGACCCGCTGCTGGGGCGGTATATCACTCAGGAACCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCG                                           <  SRR4302542.1471926/94‑1 (MQ=0)
                                                   CCGCTATTATGACCCGCTGCTGGGGCGGTATATCACTCAGGAACCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCG                                           <  SRR4302542.4660187/94‑1 (MQ=0)
                                                   CCGCTAATATGACCCGCTGCTGGGGCGGTATATCACTCAGGAACCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCG                                           <  SRR4302542.5484564/94‑1 (MQ=0)
                                                    CGCTATTATGACCCGCTGCTGGGGCGGTATATCACTCAGGAACCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATTCGTTGAATCCGG                                          <  SRR4302542.6250530/94‑1 (MQ=1)
                                                    CGCTATTATGACCCGCTGCTGGGGCGGTATATCACTCAGGAACCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGG                                          <  SRR4302542.6250529/94‑1 (MQ=2)
                                                    CGCTATTATGACCCGCTGCTGGGGCGGTATATCACTCAGGAACCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGG                                          >  SRR4302542.2298682/1‑94 (MQ=2)
                                                     GCTATTATGACCCGCTGCTGGGGCGGTATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGT                                         <  SRR4302542.6089720/94‑1 (MQ=4)
                                                     GCTATTATGACCCGCTGCTGGGGCGGTATATCACTCAGGAACCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGT                                         >  SRR4302542.7481231/1‑94 (MQ=4)
                                                     GCTATTATGACCCGCTGCTGGGGCGGTATATCACTCAGGAACCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGT                                         >  SRR4302542.6111947/1‑94 (MQ=4)
                                                      CTATTATGACCCGCTGCTGGGGCGGTATATCACTCAGGAACCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTC                                        >  SRR4302542.3145939/1‑94 (MQ=6)
                                                      CTATTATGACCCGCTGCTGGGGCGGTATATCACTCAGGAACCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTC                                        <  SRR4302542.1910158/94‑1 (MQ=6)
                                                      CTATTATGACCCGCTGCTGGGGCGGTATATCACTCAGGAACCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTC                                        <  SRR4302542.1809284/94‑1 (MQ=6)
                                                      CTATTATGACCCGCTGCTGGGGCGGTATATCACTCAGGAACCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTC                                        >  SRR4302542.1857809/1‑94 (MQ=6)
                                                       TATTATGACCCGCTGCTGGGGCGGTATATCACTCAGGAACCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCA                                       <  SRR4302542.520299/94‑1 (MQ=9)
                                                       TATTATGACCCGCTGCTGGGGCGGTATATCACTCAGGAACCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCA                                       <  SRR4302542.5425827/94‑1 (MQ=9)
                                                         TTATGACCCGCTGCTGGGGCGGTATATCACTCAGGAACCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATA                                     >  SRR4302542.7193817/1‑94 (MQ=13)
                                                          TATGACCCGCTGCTGGGGCGGTATATCACTCAGGAACCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAA                                    >  SRR4302542.6166401/1‑94 (MQ=18)
                                                          TATGACCCGCTGCTGGGGCGGTATATCACTCAGGAACCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAA                                    >  SRR4302542.2590348/1‑94 (MQ=18)
                                                           ATGACCCGCTGCTGGGGCGGTATATCACTCAGGAACCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAA                                   >  SRR4302542.1400910/1‑94 (MQ=21)
                                                             GACCCGCTGCTGGGGCGGTATATCACTCAGGAACCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATG                                 >  SRR4302542.3714242/1‑94 (MQ=25)
                                                               CCCGCTGCTGGGGCGGTATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGTA                               >  SRR4302542.943266/1‑94 (MQ=31)
                                                               CCCGCTGCTGGGGCGGTATATCACTCAGGAACCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGTA                               <  SRR4302542.6123325/94‑1 (MQ=30)
                                                               CCCGCTGCTGGGGCGGTATATCACTCAGGAACCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGTA                               <  SRR4302542.508033/94‑1 (MQ=30)
                                                                CCGCTGCTGGGGCGGTATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGTAG                              >  SRR4302542.3134739/1‑94 (MQ=34)
                                                                CCGCTGCTGGGGCGGTATATCACTCAGGAACCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGTAG                              >  SRR4302542.1294344/1‑94 (MQ=32)
                                                                CCGCTGCTGGGGCGGTATATCACTCAGGAACCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGTAG                              <  SRR4302542.5275830/94‑1 (MQ=32)
                                                                CCGCTGCTGGGGCGGTATATCACTCAGGAACCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGTAG                              <  SRR4302542.7415504/94‑1 (MQ=32)
                                                                 CGCTGCTGGGGCGGTATATCACTCAGGAACCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGTAGA                             >  SRR4302542.7417424/1‑94 (MQ=35)
                                                                 CGCTGCTGGGGCGGTATATCACTCAGGAACCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGTAGA                             <  SRR4302542.3004160/94‑1 (MQ=35)
                                                                 CGCTGCTGGGGCGGTATATCACTCAGGAACCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGTAGA                             >  SRR4302542.2761913/1‑94 (MQ=35)
                                                                  GCTGCTGGGGCGGTATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGTAGAT                            >  SRR4302542.3538789/1‑94 (MQ=39)
                                                                  GCTGCTGGGGCGGTATATCACTCAGGAACCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGTAGAT                            <  SRR4302542.7050363/94‑1 (MQ=37)
                                                                  GCTGCTGGGGCGGTATATCACTCAGGAACCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGTAGAT                            <  SRR4302542.2278530/94‑1 (MQ=37)
                                                                   CTGCTGGGGCGGTATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGTAGATC                           <  SRR4302542.6185143/94‑1 (MQ=41)
                                                                   CTGCTGGGGCGGTATATCACTCAGGAACCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGTAGATC                           <  SRR4302542.1701690/94‑1 (MQ=39)
                                                                   CTGCTGGGGCGGTATATCACTCAGGAACCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGTAGATC                           <  SRR4302542.118729/94‑1 (MQ=39)
                                                                    TGCTGGGGCGGTATATCACTCAGGAACCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGTAGATCC                          <  SRR4302542.5221179/94‑1 (MQ=42)
                                                                      CTGGGGCGGTATATCACTCAGGAACCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGTAGATCCGC                        >  SRR4302542.1913538/1‑94 (MQ=47)
                                                                      CTGGGGCGGTATATCACTCAGGAACCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGTAGATCCGC                        >  SRR4302542.716691/1‑94 (MQ=47)
                                                                       TGGGGCGGTATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGTAGATCCGCA                       >  SRR4302542.4186074/1‑94 (MQ=52)
                                                                        GGGGCGGTATATCACTCAGGAACCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGTAGATCCGCAA                      <  SRR4302542.2867228/94‑1 (MQ=53)
                                                                         GGGCGGTATATCACTCAGGAACCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGTAGATCCGCAAG                     <  SRR4302542.1563479/94‑1 (MQ=55)
                                                                         GGGCGGTATATCACTCAGGAACCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGTAGATCCGCAAG                     <  SRR4302542.6161724/94‑1 (MQ=55)
                                                                         GGGCGGTATATCACTCAGGAACCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGTAGATCCGCAAG                     <  SRR4302542.4671877/94‑1 (MQ=55)
                                                                          GGCGGTATATCACTCAGGAACCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGTAGATCCGCAAGG                    <  SRR4302542.1379075/94‑1 (MQ=58)
                                                                          GGCGGTATATCACTCAGGAACCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGTAGATCCGCAAGG                    <  SRR4302542.1828776/94‑1 (MQ=58)
                                                                          GGCGGTATATCACTCAGGAACCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGTAGATCCGCAAGG                    >  SRR4302542.3202035/1‑94 (MQ=58)
                                                                            CGGTATATCACTCAGGAACCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGTAGATCCGCAAGGTT                  >  SRR4302542.3763778/1‑94 (MQ=60)
                                                                             GGTATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGTAGATCCGCAAGGTTT                 <  SRR4302542.4366511/94‑1 (MQ=60)
                                                                             GGTATATCACTCAGGAACCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGTAGATCCGCAAGGTTT                 >  SRR4302542.1841180/1‑94 (MQ=60)
                                                                             GGTATATCACTCAGGAACCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGTAGATCCGCAAGGTTT                 >  SRR4302542.613716/1‑94 (MQ=60)
                                                                             GGTATATCACTCAGGAACCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGTAGATCCGCAAGGTTT                 >  SRR4302542.811328/1‑94 (MQ=60)
                                                                              GTATATCACTCAGGAACCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGTAGATCCGCAAGGTTTG                <  SRR4302542.6658970/94‑1 (MQ=60)
                                                                              GTATATCACTCAGGAACCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGTAGATCCGCAAGGTTTG                <  SRR4302542.2090568/94‑1 (MQ=60)
                                                                              GTATATCACTCAGGAACCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGTAGATCCGCAAGGTTTG                <  SRR4302542.135905/94‑1 (MQ=60)
                                                                               TATATCACTCAGGAACCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGTAGATCCGCAAGGTTTGG               >  SRR4302542.7093470/1‑94 (MQ=60)
                                                                               TATATCACTCAGGAACCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGTAGATCCGCAAGGTTTGG               >  SRR4302542.3353710/1‑94 (MQ=60)
                                                                                ATATCACTCAGGAACCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGTAGATCCGCAAGGTTTGGT              >  SRR4302542.4694972/1‑94 (MQ=60)
                                                                                ATATCACTCAGGAACCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGTAGATCCGCAAGGTTTGGT              >  SRR4302542.2579008/1‑94 (MQ=60)
                                                                                ATATCACTCAGGAACCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGTAGATCCGCAAGGTTTGGT              >  SRR4302542.1473989/1‑94 (MQ=60)
                                                                                ATATCACTCAGGAACCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGTAGATCCGCAAGGTTTGGT              >  SRR4302542.2500417/1‑94 (MQ=60)
                                                                                 TATCACTCAGGAACCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGTAGATCCGCAAGGTTTGGTT             <  SRR4302542.3067819/94‑1 (MQ=60)
                                                                                 TATCACTCAGGAACCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGTAGATCCGCAAGGTTTGGTT             <  SRR4302542.685008/94‑1 (MQ=60)
                                                                                 TATCACTCAGGAACCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGTAGATCCGCAAGGTTTGGTT             <  SRR4302542.2904063/94‑1 (MQ=60)
                                                                                  ATCACTCAGGAACCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGTAGATCCGCAAGGTTTGGTTG            >  SRR4302542.3140551/1‑94 (MQ=60)
                                                                                   TCACTCAGGAACCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGTAGATCCGCAAGGTTTGGTTGA           <  SRR4302542.3067437/94‑1 (MQ=60)
                                                                                    CACTCAGGATCCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGTAGATCCGCAAGGTTTGGTTGAT          <  SRR4302542.3755689/94‑1 (MQ=60)
                                                                                    CACTCAGGAACCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGTAGATCCGCAAGGTTTGGTTGAT          <  SRR4302542.6777190/94‑1 (MQ=60)
                                                                                    CACTCAGGAACCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGTAGATCCGCAAGGTTTGGTTGAT          >  SRR4302542.587202/1‑94 (MQ=60)
                                                                                     ACTCAGGAACCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGTAGATCCGCAAGGTTTGGTTGATA         >  SRR4302542.6413888/1‑94 (MQ=60)
                                                                                      CTCAGGATCCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGTAGATCCGCAAGGTTTGGTTGATAT        <  SRR4302542.3205076/94‑1 (MQ=60)
                                                                                      CTCAGGAACCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGTAGATCCGCAAGGTTTGGTTGATAT        >  SRR4302542.100859/1‑94 (MQ=60)
                                                                                      CTCAGGAACCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGTAGATCCGCAAGGTTTGGTTGATAT        <  SRR4302542.5699937/94‑1 (MQ=60)
                                                                                      CTCAGGAACCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGTAGATCCGCAAGGTTTGGTTGATAT        <  SRR4302542.3984250/94‑1 (MQ=60)
                                                                                       TCAGGAACCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGTAGATCCGCAAGGTTTGGTTGATATA       <  SRR4302542.5328121/94‑1 (MQ=60)
                                                                                        CAGGAACCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGTAGATCCGCAAGGTTTGGTTGATATAA      <  SRR4302542.162447/94‑1 (MQ=60)
                                                                                        CAGGAACCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGTAGATCCGCAAGGTTTGGTTGATATAA      >  SRR4302542.570408/1‑94 (MQ=60)
                                                                                        CAGGAACCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGTAGATCCGCAAGGTTTGGTTGATATAA      >  SRR4302542.25699/1‑94 (MQ=60)
                                                                                        CAGGAACCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGTAGATCCGCAAGGTTTGGTTGATATAA      <  SRR4302542.702113/94‑1 (MQ=60)
                                                                                         AGGAACCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGTAGATCCGCAAGGTTTGGTTGATATAAA     <  SRR4302542.1605804/94‑1 (MQ=60)
                                                                                           GATCCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGTAGATCCGCAAGGTTTGGTTGATATAAATT   <  SRR4302542.1275894/94‑1 (MQ=60)
                                                                                           GAACCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGTAGATCCGCAAGGTTTGGTTGATATAAATT   <  SRR4302542.5083617/94‑1 (MQ=60)
                                                                                            ATCCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGTAGATCCGCAAGGTTTGGTTGATATAAATTT  >  SRR4302542.5753394/1‑94 (MQ=60)
                                                                                            AACCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGTAGATCCGCAAGGTTTGGTTGATATAAATTT  >  SRR4302542.132011/1‑94 (MQ=60)
                                                                                             |                                                                                            
CCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCTGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGGTATATCACTCAGGATCCGATTGGGCTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGTAGATCCGCAAGGTTTGGTTGATATAAATTT  >  NC_000913/3765132‑3765317

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑