Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1061 294.1 15065038 98.6% 14854127 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,867,186 T→C D68D (GAT→GAC)  yidS → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,867,1860TC86.0% 618.7 / 92.2 265D68D (GAT→GAC) yidShypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (18/19);  new base C (118/110);  total (136/129)
Fisher's exact test for biased strand distribution p-value = 8.60e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.65e-01

Only 100 of 265 total aligned reads displayed.

TGGATAAAAAGCACCAGTGTGGTACTGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTCACGCGTAACTAC  >  NC_000913/3867095‑3867276
                                                                                           |                                                                                          
 ggATAAAAAGCACCAGTGTGGTACTGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGACgg                                                                                          >  1:1536559/1‑93 (MQ=255)
 ggATAAAAAGCACCAGTGTGGTACTGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGACgg                                                                                          >  2:2946778/1‑93 (MQ=255)
    tAAAAAGCACCAGTGTGGTACTGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCt                                                                                       >  1:4072980/1‑93 (MQ=255)
    tAAAAAGCACCAGTGTGGTACTGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGACGGGCt                                                                                       >  2:2567193/1‑93 (MQ=255)
      aaaaGCACCAGTGTGGTACTGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGACGGGCTGa                                                                                     >  1:3480638/1‑93 (MQ=255)
       aaaGCACCAGTGTGGTACTGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGACGGGCTGAc                                                                                    >  1:7487156/1‑93 (MQ=255)
            aCCAGTGTGGTACTGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGACGGGCTGACGCTTc                                                                               <  2:3698489/93‑1 (MQ=255)
            aCCAGTGTGGTACTGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGACGGGCTGACGCTTc                                                                               >  2:3406366/1‑93 (MQ=255)
              cAGTGTGGTACTGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGACGGGCTGACGCTTCCt                                                                             <  2:3170303/93‑1 (MQ=255)
               aGTGTGGTACTGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTg                                                                            <  1:3712930/93‑1 (MQ=255)
               aGTGTGGTACTGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGACGGGCTGACGCTTCCTg                                                                            >  1:5779980/1‑93 (MQ=255)
               aGTGTGGTACTGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGACGGGCTGACGCTTCCTg                                                                            <  2:1778796/93‑1 (MQ=255)
                gtgtGGTACTGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGACGGGCTGACGCTTCCTGt                                                                           <  2:4096316/93‑1 (MQ=255)
                gtgtGGTACTGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGACGGGCTGACGCTTCCTGt                                                                           >  1:6845659/1‑93 (MQ=255)
                 tgtgGTACTGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGACGGGCTGACGCTTCCTGTc                                                                          <  2:3276430/93‑1 (MQ=255)
                 tgtgGTACTGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGACGGGCTGACGCTTCCTGTc                                                                          >  2:6117423/1‑93 (MQ=255)
                   tgGTACTGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGACGGGCTGACGCTTCCTGTCGa                                                                        <  2:4682313/93‑1 (MQ=255)
                     gTACTGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGACGGGCTGACGCTTCCTGTCGAtg                                                                      <  2:2366964/93‑1 (MQ=255)
                     gTACTGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGACGGGCTGACGCTTCCTGTCGAtg                                                                      >  2:1299641/1‑93 (MQ=255)
                     gTACTGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGACGGGCTGACGCTTCCTGTCGAtg                                                                      <  1:6518306/93‑1 (MQ=255)
                        cTGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGa                                                                   >  1:2832255/1‑93 (MQ=255)
                        cTGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGACGGGCTGACGCTTCCTGTCGATGTGa                                                                   >  1:1421465/1‑93 (MQ=255)
                         tGAAGGTTTCAGCAACCCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGACGGGCTGACGCTTCCTGTCGATGTGAt                                                                  <  1:3654964/93‑1 (MQ=255)
                         tGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGACGGGCTGACGCTTCCTGTCGATGTGAt                                                                  <  1:6644842/93‑1 (MQ=255)
                         tGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGACGGGCTGACGCTTCCTGTCGATGTGAt                                                                  >  1:822365/1‑93 (MQ=255)
                         tGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGACGGGCTGACGCTTCCTGTCGATGTGAt                                                                  <  1:5563900/93‑1 (MQ=255)
                         tGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGACGGGCTGACGCTTCCTGTCGATGTGAt                                                                  >  2:878762/1‑93 (MQ=255)
                          gAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATc                                                                 <  2:1423499/93‑1 (MQ=255)
                          gAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGACGGGCTGACGCTTCCTGTCGATGTGATc                                                                 >  1:3799570/1‑93 (MQ=255)
                           aaGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGACGGGCTGACGCTTCCTGTCGATGTGATCg                                                                >  2:1802878/1‑93 (MQ=255)
                           aaGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGACGGGCTGACGCTTCCTGTCGATGTGATCg                                                                >  2:4003033/1‑93 (MQ=255)
                            aGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGACGGGCTGACGCTTCCTGTCGATGTGATCGc                                                               <  1:4972998/93‑1 (MQ=255)
                            aGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGACGGGCTGACGCTTCCTGTCGATGTGATCGc                                                               >  1:3332324/1‑93 (MQ=255)
                             ggTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGACGGGCTGACGCTTCCTGTCGATGTGATCGCt                                                              >  1:5368915/1‑93 (MQ=255)
                               tttCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCGGCGATCTTTTATTCGCGACGGGCTGACGCTTCCTGTCGATGTGATCGCTaa                                                            <  1:2972890/93‑1 (MQ=255)
                                ttCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGACGGGCTGACGCTTCCTGTCGATGTGATCGCTAAt                                                           <  1:2497352/93‑1 (MQ=255)
                                 tCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATc                                                          <  1:5194652/93‑1 (MQ=255)
                                 tCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGACGGGCTGACGCTTCCTGTCGATGTGATCGCTAATc                                                          <  2:4294867/93‑1 (MQ=255)
                                   aGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGACGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCg                                                        >  2:5777418/1‑93 (MQ=255)
                                    gCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGACGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGc                                                       >  1:2563674/1‑93 (MQ=255)
                                     cAACCCCTGCGGCGGCCGGCTGCCCCCGGACGCGCAGCGATCTTTTATTCGCGCCGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCa                                                      <  1:2366567/93‑1 (MQ=255)
                                     cAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGACGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCa                                                      >  1:1792475/1‑93 (MQ=255)
                                     cAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGACGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCa                                                      <  1:6173128/93‑1 (MQ=255)
                                       aaCCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGACGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGa                                                    >  2:4181353/1‑93 (MQ=255)
                                        aCCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGACGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGAt                                                   >  1:4703280/1‑93 (MQ=255)
                                         cccTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGACGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGAtt                                                  >  1:3085610/1‑93 (MQ=255)
                                          ccTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGAttt                                                 >  1:1699766/1‑93 (MQ=255)
                                          ccTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGACGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGAttt                                                 >  1:1097712/1‑93 (MQ=255)
                                          ccTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGACGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGAttt                                                 >  1:4651106/1‑93 (MQ=255)
                                          ccTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGACGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGAttt                                                 <  1:4741485/93‑1 (MQ=255)
                                           cTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGACGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGAtttt                                                <  2:6721380/93‑1 (MQ=255)
                                           cTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGACGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGAtttt                                                >  2:506984/1‑93 (MQ=255)
                                            tGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGACGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTc                                               <  2:2010428/93‑1 (MQ=255)
                                               ggcggTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGACGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGc                                            <  2:2510836/93‑1 (MQ=255)
                                                  ggTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGACGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCgcc                                         <  1:7233095/93‑3 (MQ=255)
                                                   gTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGACGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGCCa                                        <  2:621840/93‑1 (MQ=255)
                                                       gctgGCACCGGACGCGCAGCGATCTTTTATTCGCGACGGGCTGGCGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGCCAAAAc                                    <  2:499598/93‑1 (MQ=255)
                                                       gctgGCACCGGACGCGCAGCGATCTTTTATTCGCGACGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGCCAAAAc                                    >  2:7263164/1‑93 (MQ=255)
                                                       gctgGCACCGGACGCGCAGCGATCTTTTATTCGCGACGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGCCAAAAc                                    >  1:2690703/1‑93 (MQ=255)
                                                        ctgGCACCGGACGCGCAGCGATCTTTTATTCGCGACGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGCCAAAAcc                                   <  1:5603708/93‑1 (MQ=255)
                                                         tgGCACCGGACGCGCAGCGATCTTTTATTCGCGACGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGCCAAAACCg                                  >  1:739366/1‑93 (MQ=255)
                                                          ggCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGt                                 <  2:4620654/93‑1 (MQ=255)
                                                          ggCACCGGACGCGCAGCGATCTTTTATTCGCGACGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTTAGCGCCAAAACCGt                                 <  2:6446407/93‑1 (MQ=255)
                                                          ggCACCGGACGCGCAGCGATCTTTTATTCGCGACGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGCCAAAACCGt                                 <  2:2311735/93‑1 (MQ=255)
                                                          ggCACCGGACGCGCAGCGATCTTTTATTCGCGACGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGCCAAAACCGt                                 <  2:3110769/93‑1 (MQ=255)
                                                           gCACCGGACGCGCAGCGATCTTTTATTCGCGACGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGCCAAAACCGTc                                >  2:6203157/1‑93 (MQ=255)
                                                             aCCGGACGCGCAGCGATCTTTTATTCGCGACGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGCCAAAACCGTCGa                              >  2:5475905/1‑93 (MQ=255)
                                                             aCCGGACGCGCAGCGATCTTTTATTCGCGACGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGCCAAAACCGTCGa                              <  2:380313/93‑1 (MQ=255)
                                                              ccGGACGCGCAGCGATCTTTTATTCGCGACGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGCCAAAACCGTCGAc                             >  2:156953/1‑93 (MQ=255)
                                                              ccGGACGCGCAGCGATCTTTTATTCGCGACGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGCCAAAACCGTCGAc                             <  1:6914654/93‑1 (MQ=255)
                                                                    gcgcAGCGATCTTTTATTCGCGACGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGCCAAAACCGTCGACGTcgcc                       <  2:1338577/93‑1 (MQ=255)
                                                                    gcgcAGCGATCTTTTATTCGCGACGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGCCAAAACCGTCGACGTcgcc                       <  1:6982369/93‑1 (MQ=255)
                                                                      gcAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTcgccgc                     >  1:1090071/1‑93 (MQ=255)
                                                                      gcAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTcgccgc                     >  2:1859334/1‑93 (MQ=255)
                                                                      gcAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTcgccgc                     >  1:2055760/1‑93 (MQ=255)
                                                                      gcAGCGATCTTTTATTCGCGACGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGCCAAAACCGTCGACGTcgccgc                     >  2:6363492/1‑93 (MQ=255)
                                                                      gcAGCGATCTTTTATTCGCGACGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGCCAAAACCGTCGACGTcgccgc                     >  1:5964321/1‑93 (MQ=255)
                                                                      gcAGCGATCTTTTATTCGCGACGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGCCAAAACCGTCGACGTcgccgc                     >  2:288235/1‑93 (MQ=255)
                                                                        aGCGATCTTTTATTCGCGACGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGCCAAAACCGTCGACGTCGCCGCAt                   <  1:3411977/93‑1 (MQ=255)
                                                                         gCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATc                  >  2:6922619/1‑93 (MQ=255)
                                                                         gCGATCTTTTATTCGCGACGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGCCAAAACCGTCGACGTCGCCGCATc                  >  1:5151761/1‑93 (MQ=255)
                                                                         gCGATCTTTTATTCGCGACGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGCCAAAACCGTCGACGTCGCCGCATc                  >  2:5995330/1‑93 (MQ=255)
                                                                           gATCTTTTATTCGCGACGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGc                <  2:3354051/93‑1 (MQ=255)
                                                                            aTCTTTTATTCGCGACGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGCCAAAACCGTCGACGTCGCCGCATCGCt               >  1:3569756/1‑93 (MQ=255)
                                                                            aTCTTTTATTCGCGACGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGCCAAAACCGTCGACGTCGCCGCATCGCt               >  2:7119810/1‑93 (MQ=255)
                                                                             tCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTc              <  1:3258233/93‑1 (MQ=255)
                                                                             tCTTTTATTCGCGACGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGCCAAAACCGTCGACATCGCCGCATCGCTc              >  2:3466500/1‑93 (MQ=255)
                                                                              cTTTTATTCGCGACGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGCCAAAACCGTCGACGTCGCCGCATCGCTCa             <  2:112796/93‑1 (MQ=255)
                                                                              cTTTTATTCGCGACGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGCCAAAACCGTCGACGTCGCCGCATCGCTCa             >  1:2219630/1‑93 (MQ=255)
                                                                              cTTTTATTCGCGACGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGCCAAAACCGTCGACGTCGCCGCATCGCTCa             <  1:1889272/93‑1 (MQ=255)
                                                                              cTTTTATTCGCGACGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGCCAAAACCGTCGACGTCGCCGCATCGCTCa             <  1:3171735/93‑1 (MQ=255)
                                                                               ttttATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTCAc            >  1:6070518/1‑93 (MQ=255)
                                                                               ttttATTCGCGACGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGCCAAAACCGTCGACGTCGCCGCATCGCTCAc            <  1:3525909/93‑1 (MQ=255)
                                                                               ttttATTCGCGACGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGCCAAAACCGTCGACGTCGCCGCATCGCTCAc            >  2:5641200/1‑93 (MQ=255)
                                                                                  tATTCGCGACGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGCCAAAACCGTCGACGTCGCCGCATCGCTCAcgcg         >  2:6665208/1‑93 (MQ=255)
                                                                                   aTTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTCACGCGt        <  1:6430253/93‑1 (MQ=255)
                                                                                   aTTCGCGACGGGCTGACGCTTCCTGTCGATGTGATCGCTAAACCGCAGATTTTCAGCGCCAAAACCGTCGACGTCGCCGCATCGCTCACGCGt        >  2:6544186/1‑93 (MQ=255)
                                                                                    ttCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTCACGCGTa       >  1:611511/1‑93 (MQ=255)
                                                                                     tCGCGACGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGCCAAAACCGTCGACGTCGCCGCATCGCTCACGCGTaa      >  2:4393902/1‑93 (MQ=255)
                                                                                         gATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTCACGCGTAACTAc  <  2:2688420/93‑1 (MQ=255)
                                                                                           |                                                                                          
TGGATAAAAAGCACCAGTGTGGTACTGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTCACGCGTAACTAC  >  NC_000913/3867095‑3867276

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GCTGGATAAAAAGCACCAGTGTGGTACTGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTCACGCGTAACTACCAG  >  NC_000913/3867093‑3867279
                                                                                             |                                                                                             
GCTGGATAAAAAGCACCAGTGTGGTACTGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGAC                                                                                               >  SRR4302542.415649/1‑94 (MQ=60)
 CTGGATAAAAAGCACCCGTGTGGTACTGAAAGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCAGACGCGCAGCGATCTTTTATTCGCGACG                                                                                              >  SRR4302542.6932719/1‑94 (MQ=60)
 CTGGATAAAAAGCACCAGTGTGGTACTGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGACG                                                                                              >  SRR4302542.1092874/1‑94 (MQ=60)
 CTGGATAAAAAGCACCAGTGTGGTACTGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGACG                                                                                              >  SRR4302542.7158961/1‑94 (MQ=60)
 CTGGATAAAAAGCACCAGTGTGGTACTGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGACG                                                                                              >  SRR4302542.2681060/1‑94 (MQ=60)
 CTGGATAAAAAGCACCAGTGTGGTACTGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGACG                                                                                              >  SRR4302542.6444113/1‑94 (MQ=60)
 CTGGATAAAAAGCACCAGTGTGGTACTGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGACG                                                                                              <  SRR4302542.842612/94‑1 (MQ=60)
 CTGGATAAAAAGCACCAGTGTGGTACTAAAGGTTTCAGCAACCCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGATG                                                                                              >  SRR4302542.808088/1‑94 (MQ=60)
  TGGATAAAAAGCACCAGTGTGGTACTGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGATGG                                                                                             >  SRR4302542.6656311/1‑94 (MQ=60)
  TGGATAAAAAGCACCAGTGTGGTACTGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGACGG                                                                                             >  SRR4302542.1549831/1‑94 (MQ=60)
    GATAAAAAGCACCAGTGTGGTACTGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGC                                                                                           >  SRR4302542.3321887/1‑94 (MQ=60)
     ATAAAAAGCTCCAGTGTTGTACTGAAGGTTTCAGCAAACCGTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGACGGGCT                                                                                          <  SRR4302542.6569377/94‑1 (MQ=60)
     ATAAAAAGCACCAGTGTGGTACTGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCT                                                                                          >  SRR4302542.4113137/1‑94 (MQ=60)
     ATAAAAAGCACCAGTGTGGTACTGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGACGGGCT                                                                                          <  SRR4302542.5459359/94‑1 (MQ=60)
       AAAAAGCACCAGTGTGGTACTGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGACGGGCTGA                                                                                        >  SRR4302542.1515413/1‑94 (MQ=60)
       AAAAAGCACCAGTGTGGTACTGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGACGGGCTGA                                                                                        >  SRR4302542.47693/1‑94 (MQ=60)
       AAAAAGCACCAGTGTGGTACTGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGACGGGCTGA                                                                                        >  SRR4302542.3513213/1‑94 (MQ=60)
        AAAAGCACCAGTGTGGTACTGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGACGGGCTGAC                                                                                       >  SRR4302542.7560584/1‑94 (MQ=60)
        AAAAGCACCAGTGTGGTACTGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGACGGGCTGAC                                                                                       <  SRR4302542.1373478/94‑1 (MQ=60)
         AAAGCACCAGTGTGGTACTGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGACGGGCTGACG                                                                                      <  SRR4302542.3717177/94‑1 (MQ=60)
         AAAGCACCAGTGTGGTACTGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGACGGGCTGACG                                                                                      >  SRR4302542.2520436/1‑94 (MQ=60)
           AGCACCAGTGTGGTACTGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGACGGGCTGACGCT                                                                                    >  SRR4302542.1206230/1‑94 (MQ=60)
           AGCACCAGTGTGGTACTGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGACGGGCTGACGCT                                                                                    >  SRR4302542.1754528/1‑94 (MQ=60)
              ACCAGTGTGGTACTGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGACGGGCTGACGCTTCC                                                                                 >  SRR4302542.3120777/1‑94 (MQ=60)
                CAGTGTGGTACTGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGACGGGCTGACGCTTCCTG                                                                               >  SRR4302542.5836102/1‑94 (MQ=60)
                 AGTGTGGTACTGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGT                                                                              <  SRR4302542.3749663/94‑1 (MQ=60)
                 AGTGTGGTACTGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGACGGGCTGACGCTTCCTGT                                                                              >  SRR4302542.6912898/1‑94 (MQ=60)
                  GTGTGGTACTGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGACGGGCTGACGCTTCCTGTC                                                                             <  SRR4302542.2120036/94‑1 (MQ=60)
                   TGTGGTACTGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGACGGGCTGACGCTTCCTGTCG                                                                            >  SRR4302542.441815/1‑94 (MQ=60)
                   TGTGGTACTGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGACGGGCTGACGCTTCCTGTCG                                                                            >  SRR4302542.3931687/1‑94 (MQ=60)
                     TGGTACTGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGACGGGCTGACGCTTCCTGTCGAT                                                                          <  SRR4302542.6759069/94‑1 (MQ=60)
                       GTACTGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGACGGGCTGACGCTTCCTGTCGATGT                                                                        <  SRR4302542.6810852/94‑1 (MQ=60)
                       GTACTGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGACGGGCTGACGCTTCCTGTCGATGT                                                                        <  SRR4302542.6582542/94‑1 (MQ=60)
                         ACTGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGA                                                                      >  SRR4302542.2859478/1‑94 (MQ=60)
                         ACTGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGACGGGCTGACGCTTCCTGTCGATGTGA                                                                      >  SRR4302542.1433773/1‑94 (MQ=60)
                          CTGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGACGGGCTGACGCTTCCTGTCGATGTGNN                                                                     >  SRR4302542.3271168/1‑94 (MQ=60)
                          CTGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGACGGGCTGACGCTTCCTGTCGATGTGAT                                                                     <  SRR4302542.6520504/94‑1 (MQ=60)
                          CTGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGACGGGCTGACGCTTCCTGTCGATGTGAT                                                                     >  SRR4302542.829473/1‑94 (MQ=60)
                          CTGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGACGGGCTGACGCTTCCTGTCGATGTGAT                                                                     >  SRR4302542.5686715/1‑94 (MQ=60)
                          CTGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGACGGGCTGACGCTTCCTGTCGATGTGAT                                                                     >  SRR4302542.4857290/1‑94 (MQ=60)
                           TGAAGGTTTCAGCAACCCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGACGGGCTGACGCTTCCTGTCGATGTGATC                                                                    <  SRR4302542.3689211/94‑1 (MQ=60)
                           TGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGACGGGCTGACGCTTCCTGTCGATGTGATC                                                                    >  SRR4302542.3535792/1‑94 (MQ=60)
                           TGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGACGGGCTGACGCTTCCTGTCGATGTGATC                                                                    <  SRR4302542.6710335/94‑1 (MQ=60)
                           TGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGACGGGCTGACGCTTCCTGTCGATGTGATC                                                                    <  SRR4302542.5617963/94‑1 (MQ=60)
                           TGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGACGGGCTGACGCTTCCTGTCGATGTGATC                                                                    >  SRR4302542.3837181/1‑94 (MQ=60)
                           TGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGACGGGCTGACGCTTCCTGTCGATGTGATC                                                                    <  SRR4302542.5434232/94‑1 (MQ=60)
                           TGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGACGGGCTGACGCTTCCTGTCGATGCGATC                                                                    <  SRR4302542.7041364/94‑1 (MQ=60)
                            GAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGACGGGCTGACGCTTCCTGTCGATGTGATCG                                                                   >  SRR4302542.3364965/1‑94 (MQ=60)
                             AAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGACGGGCTGACGCTTCCTGTCGATGTGATCGC                                                                  >  SRR4302542.6194024/1‑94 (MQ=60)
                             AAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGACGGGCTGACGCTTCCTGTCGATGTGATCGC                                                                  >  SRR4302542.3363685/1‑94 (MQ=60)
                              AGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGACGGGCTGACGCTTCCTGTCGATGTGATCGCT                                                                 <  SRR4302542.5021277/94‑1 (MQ=60)
                              AGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGACGGGCTGACGCTTCCTGTCGATGTGATCGCT                                                                 <  SRR4302542.3484347/94‑1 (MQ=60)
                              AGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGACGGGCTGACGCTTCCTGTCGATGTGATCGCT                                                                 >  SRR4302542.5421093/1‑94 (MQ=60)
                               GGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTA                                                                <  SRR4302542.1985944/94‑1 (MQ=60)
                               GGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGACGGGCTGACGCTTCCTGTCGATGTGATCGCTA                                                                >  SRR4302542.5347207/1‑94 (MQ=60)
                                 TTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCGGCGATCTTTTATTCGCGACGGGCTGACGCTTCCTGTCGATGTGATCGCTAAT                                                              <  SRR4302542.3001363/94‑1 (MQ=60)
                                  TTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGACGGGCTGACGCTTCCTGTCGATGTGATCGCTAATC                                                             <  SRR4302542.2521571/94‑1 (MQ=60)
                                   TCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCC                                                            <  SRR4302542.3808736/94‑1 (MQ=60)
                                   TCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCC                                                            <  SRR4302542.5245126/94‑1 (MQ=60)
                                   TCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGACGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCC                                                            <  SRR4302542.7162806/94‑1 (MQ=60)
                                    CAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGACGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCG                                                           >  SRR4302542.179604/1‑94 (MQ=60)
                                     AGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGACGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGC                                                          >  SRR4302542.2588527/1‑94 (MQ=60)
                                      GCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGACGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCA                                                         >  SRR4302542.1808027/1‑94 (MQ=60)
                                       CAACCCCTGCGGCGGCCGGCTGCCCCCGGACGCGCAGCGATCTTTTATTCGCGCCGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAG                                                        <  SRR4302542.2387104/94‑1 (MQ=60)
                                       CAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGACGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAG                                                        <  SRR4302542.6130570/94‑1 (MQ=60)
                                       CAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGACGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAG                                                        <  SRR4302542.6232959/94‑1 (MQ=60)
                                       CAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGACGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAG                                                        >  SRR4302542.2199344/1‑94 (MQ=60)
                                       CAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGACGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAG                                                        >  SRR4302542.1105441/1‑94 (MQ=60)
                                         AACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGAT                                                      <  SRR4302542.3417616/94‑1 (MQ=60)
                                         AACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGACGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGAT                                                      >  SRR4302542.4749175/1‑94 (MQ=60)
                                         AACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGACGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGAT                                                      <  SRR4302542.7394829/94‑1 (MQ=60)
                                         AACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGACGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGAT                                                      <  SRR4302542.361802/94‑1 (MQ=60)
                                         AACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGACGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGAT                                                      <  SRR4302542.7074638/94‑1 (MQ=60)
                                          ACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGACGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATT                                                     >  SRR4302542.3114983/1‑94 (MQ=60)
                                           CCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTT                                                    >  SRR4302542.1714497/1‑94 (MQ=60)
                                           CCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGACGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTT                                                    >  SRR4302542.1107117/1‑94 (MQ=60)
                                           CCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGACGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTT                                                    <  SRR4302542.6921082/94‑1 (MQ=60)
                                           CCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGACGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTT                                                    >  SRR4302542.4696576/1‑94 (MQ=60)
                                            CCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGACGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTT                                                   <  SRR4302542.4787713/94‑1 (MQ=60)
                                             CTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGACGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTC                                                  >  SRR4302542.5866386/1‑94 (MQ=60)
                                             CTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGACGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTC                                                  >  SRR4302542.7470148/1‑94 (MQ=60)
                                               GCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGACGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAG                                                <  SRR4302542.3783666/94‑1 (MQ=60)
                                                CGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGACGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGC                                               >  SRR4302542.4747218/1‑94 (MQ=60)
                                                 GGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGACGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCG                                              <  SRR4302542.5782938/94‑1 (MQ=60)
                                                   CGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGACGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGCC                                            >  SRR4302542.6309810/1‑94 (MQ=60)
                                                    GGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGACGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGCCA                                           <  SRR4302542.7304043/94‑1 (MQ=60)
                                                    GGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGACGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGCCA                                           >  SRR4302542.2650846/1‑94 (MQ=60)
                                                     GTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGACGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGCCAA                                          <  SRR4302542.308814/94‑1 (MQ=60)
                                                     GTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGACGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGCCAA                                          >  SRR4302542.2840323/1‑94 (MQ=60)
                                                      TCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAA                                         <  SRR4302542.82258/94‑1 (MQ=60)
                                                       CTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGACGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGCCAAAA                                        >  SRR4302542.1620475/1‑94 (MQ=60)
                                                       CTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGACGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGCCAAAA                                        >  SRR4302542.3179252/1‑94 (MQ=60)
                                                        TGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGACGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGCCAAAAC                                       >  SRR4302542.2716650/1‑94 (MQ=60)
                                                         GCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACC                                      <  SRR4302542.6221883/94‑1 (MQ=60)
                                                          CTGGCACCGGACGCGCAGCGATCTTTTATTCGCGACGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGCCAAAACCG                                     <  SRR4302542.5658150/94‑1 (MQ=60)
                                                          CTGGCACCGGACGCGCAGCGATCTTTTATTCGCGACGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGCCAAAACCG                                     >  SRR4302542.6023791/1‑94 (MQ=60)
                                                          CTGGCACCGGACGCGCAGCGATCTTTTATTCGCGACGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGCCAAAACCG                                     <  SRR4302542.5074164/94‑1 (MQ=60)
                                                          CTGGCACCGGACGCGCAGCGATCTTTTATTCGCGACGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGCCAAAACCG                                     >  SRR4302542.745784/1‑94 (MQ=60)
                                                          CTGGCACCGGACGCGCAGCGATCTTTTATTCGCGACGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGCCAAAACCG                                     <  SRR4302542.2971032/94‑1 (MQ=60)
                                                            GGCACCGGACGCGCAGCGATCTTTTATTCGCGACGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGCCAAAACCGTC                                   >  SRR4302542.2739444/1‑94 (MQ=60)
                                                            GGCACCGGACGCGCAGCGATCTTTTATTCGCGACGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGCCAAAACCGTC                                   <  SRR4302542.2870573/94‑1 (MQ=60)
                                                            GGCACCGGACGCGCAGCGATCTTTTATTCGCGACGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGCCAAAACCGTC                                   <  SRR4302542.3568297/94‑1 (MQ=60)
                                                             GCACCGGACGCGCAGCGATCTTTTATTCGCGACGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGCCAAAACCGTCG                                  <  SRR4302542.328546/94‑1 (MQ=60)
                                                                CCGGACGCGCAGCGATCTTTTATTCGCGACGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGCCAAAACCGTCGACG                               <  SRR4302542.6982533/94‑1 (MQ=60)
                                                                  GGACGCGCAGCGATCTTTTATTCGCGACGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGCCAAAACCGTCGACGTC                             <  SRR4302542.1978740/94‑1 (MQ=60)
                                                                   GACGCGCAGCGATCTTTTATTCGCGACGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGCCAAAACCGTCGACGTCG                            <  SRR4302542.5651165/94‑1 (MQ=60)
                                                                   GACGCGCAGCGATCTTTTATTCGCGACGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGCCAAAACCGTCGACGTCG                            <  SRR4302542.5121961/94‑1 (MQ=60)
                                                                     CGCGCAGCGATCTTTTATTCGCGACGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGCCAAAACCGTCGACGTCGCC                          <  SRR4302542.3510483/94‑1 (MQ=60)
                                                                      GCGCAGCGATCTTTTATTCGCGACGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGCCAAAACCGTCGACGTCGCCG                         <  SRR4302542.7050944/94‑1 (MQ=60)
                                                                       CGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGC                        >  SRR4302542.1099414/1‑94 (MQ=60)
                                                                       CGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGC                        >  SRR4302542.2073524/1‑94 (MQ=60)
                                                                       CGCAGCGATCTTTTATTCGCGACGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGCCAAAACCGTCGACGTCGCCGC                        >  SRR4302542.6022065/1‑94 (MQ=60)
                                                                       CGCAGCGATCTTTTATTCGCGACGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGCCAAAACCGTCGACGTCGCCGC                        >  SRR4302542.5213876/1‑94 (MQ=60)
                                                                        GCAGCGATCTTTTATTCGCGACGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGCCAAAACCGTCGACGTCGCCGCA                       <  SRR4302542.3726955/94‑1 (MQ=60)
                                                                          AGCGATCTTTTATTCGCGACGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGCCAAAACCGTCGACGTCGCCGCATC                     <  SRR4302542.237982/94‑1 (MQ=60)
                                                                          AGCGATCTTTTATTCGCGACGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGCCAAAACCGTCGACGTCGCCGCATC                     <  SRR4302542.3443949/94‑1 (MQ=60)
                                                                          AGCGATCTTTTATTCGCGACGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGCCAAAACCGTCGACGTCGCCGCATC                     >  SRR4302542.1678452/1‑94 (MQ=60)
                                                                          AGCGATCTTTTATTCGCGACGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGCCAAAACCGTCGACGTCGCCGCATC                     >  SRR4302542.5201835/1‑94 (MQ=60)
                                                                           GCGATCTTTTATTCGCGACGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGCCAAAACCGTCGACGTCGCCGCATCG                    <  SRR4302542.2582598/94‑1 (MQ=60)
                                                                            CGATCTTTTATTCGCGACGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGCCAAAACCGTCGACGTCGCCGCATCGC                   >  SRR4302542.3571376/1‑94 (MQ=60)
                                                                             GATCTTTTATTCGCGACGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGCCAAAACCGTCGACGTCGCCGCATCGCT                  >  SRR4302542.6136485/1‑94 (MQ=60)
                                                                             GATCTTTTATTCGCGACGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGCCAAAACCGTCGACGTCGCCGCATCGCT                  >  SRR4302542.3603074/1‑94 (MQ=60)
                                                                               TCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTCA                <  SRR4302542.3289007/94‑1 (MQ=60)
                                                                               TCTTTTATTCGCGACGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGCCAAAACCGTCGACGTCGCCGCATCGCTCA                >  SRR4302542.4945405/1‑94 (MQ=60)
                                                                               TCTTTTATTCGCGACGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGCCAAAACCGTCGACGTCGCCGCATCGCTCA                >  SRR4302542.2238724/1‑94 (MQ=60)
                                                                               TCTTTTATTCGCGACGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGCCAAAACCGTCGACGTCGCCGCATCGCTCA                >  SRR4302542.5839043/1‑94 (MQ=60)
                                                                                CTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTCAC               >  SRR4302542.6129312/1‑94 (MQ=60)
                                                                                CTTTTATTCGCGACGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGCCAAAACCGTCGACGTCGCCGCATCGCTCAC               >  SRR4302542.2518644/1‑94 (MQ=60)
                                                                                CTTTTATTCGCGACGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGCCAAAACCGTCGACGTCGCCGCATCGCTCAC               <  SRR4302542.3201816/94‑1 (MQ=60)
                                                                                CTTTTATTCGCGACGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGCCAAAACCGTCGACGTCGCCGCATCGCTCAC               <  SRR4302542.5404298/94‑1 (MQ=60)
                                                                                CTTTTATTCGCGACGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGCCAAAACCGTCGACGTCGCCGCATCGCTCAC               >  SRR4302542.1825472/1‑94 (MQ=60)
                                                                                CTTTTATTCGCGACGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGCCAAAACCGTCGACGTCGCCGCATCGCTCAC               <  SRR4302542.1905623/94‑1 (MQ=60)
                                                                                 TTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTCACG              <  SRR4302542.6928624/94‑1 (MQ=60)
                                                                                 TTTTATTCGCGACGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGCCAAAACCGTCGACGTCGCCGCATCGCTCACG              <  SRR4302542.3558862/94‑1 (MQ=60)
                                                                                 TTTTATTCGCGACGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGCCAAAACCGTCGACGTCGCCGCATCGCTCACG              >  SRR4302542.4721107/1‑94 (MQ=60)
                                                                                   TTATTCGCGACGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGCCAAAACCGTCGACGTCGCCGCATCGCTCACGCG            >  SRR4302542.4676640/1‑94 (MQ=60)
                                                                                   TTATTCGCGACGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGCCAAAACCGTCGACGTCGCCGCATCGCTCACGCG            <  SRR4302542.2533030/94‑1 (MQ=60)
                                                                                    TATTCGCGACGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGCCAAAACCGTCGACGTCGCCGCATCGCTCACGCGT           <  SRR4302542.738046/94‑1 (MQ=60)
                                                                                     ATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTCACGCGTA          >  SRR4302542.616771/1‑94 (MQ=60)
                                                                                     ATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTCACGCGTA          <  SRR4302542.6493607/94‑1 (MQ=60)
                                                                                          CGACGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGCCAAAACCGTCGACGTCGCCGCATCGCTCACGCGTAACTAC     >  SRR4302542.2862971/1‑94 (MQ=60)
                                                                                           GACGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGCCAAAACCGTCGACGTCGCCGCATCGCTCACGCGTAACTACC    >  SRR4302542.2945383/1‑94 (MQ=60)
                                                                                           GACGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGCCAAAACCGTCGACGTCGCCGCATCGCTCACGCGTAACTACC    <  SRR4302542.6411602/94‑1 (MQ=60)
                                                                                             CGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGCCAAAACCGTCGACGTCGCCGCATCGCTCACGCGTAACTACCAG  <  SRR4302542.4084061/94‑1 (MQ=60)
                                                                                             CGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGCCAAAACCGTCGACGTCGCCGCATCGCTCACGCGTAACTACCAG  <  SRR4302542.5058488/94‑1 (MQ=60)
                                                                                             CGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGCCAAAACCGTCGACGTCGCCGCATCGCTCACGCGTAACTACCAG  >  SRR4302542.1303850/1‑94 (MQ=60)
                                                                                             |                                                                                             
GCTGGATAAAAAGCACCAGTGTGGTACTGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTCACGCGTAACTACCAG  >  NC_000913/3867093‑3867279

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑