Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	1061	294.1	15065038	98.6%	14854127	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,873,209	A→G	T30A (ACT→GCT)	yidX →	putative replicase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,873,209	0	A	G	85.0%	716.6 / 114.8	306	T30A (ACT→GCT)	yidX	putative replicase
Reads supporting (aligned to +/- strand): ref base A (25/21); new base G (146/114); total (171/135)
Fisher's exact test for biased strand distribution p-value = 8.73e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01

Only 100 of 307 total aligned reads displayed.

GCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTGGATTACCTTATCACTGATGGTGCCGAT  >  NC_000913/3873118‑3873301

gcTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCAc                                                                                             >  1:4851972/1‑93 (MQ=255)

 cTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCGCt                                                                                            >  1:1687501/1‑93 (MQ=255)

  tGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTg                                                                                           >  2:6082993/1‑93 (MQ=255)

   gATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCTATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCGCTgg                                                                                          >  2:4710566/1‑93 (MQ=255)

   gATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCGCttg                                                                                          >  2:6566734/1‑91 (MQ=255)

   gATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCGCTgg                                                                                          <  1:6760912/93‑1 (MQ=255)

   gATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCGCTgg                                                                                          <  1:1924160/93‑1 (MQ=255)

   gATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCGCTgg                                                                                          >  2:1476618/1‑93 (MQ=255)

    aTGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCGCTggg                                                                                         <  2:4647907/93‑1 (MQ=255)

    aTGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCGCTggg                                                                                         >  1:4078403/1‑93 (MQ=255)

    aTGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCGCTggg                                                                                         >  2:4043179/1‑93 (MQ=255)

      gCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCGCTGGGCt                                                                                       <  1:6476224/93‑1 (MQ=255)

          tCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATCg                                                                                   >  2:5336406/1‑93 (MQ=255)

           cAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCGCTGGGCTATCGc                                                                                  <  2:6026560/93‑1 (MQ=255)

            aGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCGCTGGGCTATCGCa                                                                                 <  1:1503688/93‑1 (MQ=255)

            aGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCGCTGGGCTATCGCa                                                                                 <  2:1549614/93‑1 (MQ=255)

             ggCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCGCTGGGCTATCGCAg                                                                                <  2:4322399/93‑1 (MQ=255)

             ggCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCGCTGGGCTATCGCAg                                                                                <  2:4994/93‑1 (MQ=255)

             ggCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATCGCAg                                                                                <  1:3314160/93‑1 (MQ=255)

              gCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCGCTGGGCTATCGCAgg                                                                               >  2:26950/1‑93 (MQ=255)

                 gTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCGCTGGGCTATCGCAGGCaa                                                                            <  2:3833173/93‑1 (MQ=255)

                  tATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCGCTGGGCTATCGCAGGCaaa                                                                           >  1:4711167/1‑93 (MQ=255)

                  tATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCGCTGGGCTATCGCAGGCaaa                                                                           >  2:7018787/1‑93 (MQ=255)

                    tctcGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCGCTGGGCTATCGCAGGCAAAAg                                                                         <  1:3044273/93‑1 (MQ=255)

                     ctcGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCGCTGGGCTATCGCAGGCAAAAGa                                                                        <  1:3726295/93‑1 (MQ=255)

                     ctcGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGa                                                                        >  2:4229124/1‑93 (MQ=255)

                      tcGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCGCTGGGCTATCGCAGGCAAAAGAt                                                                       >  1:3531401/1‑93 (MQ=255)

                       cGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCGCTGGGCTATCGCAGGCAAAAGATa                                                                      <  1:5264868/93‑1 (MQ=255)

                         tATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCGCTGGGCTATCGCAGGCAAAAGATAGt                                                                    >  1:1361432/1‑93 (MQ=255)

                         tATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCGCTGGGCTATCGCAGGCAAAAGATAGt                                                                    >  1:121794/1‑93 (MQ=255)

                         tATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCACACACCATCGCTGGGCTATCGCAGGCAAAAGATAGt                                                                    >  2:4790989/1‑93 (MQ=255)

                          aTGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCGCTGGGCTATCGCAGGCAAAAGATAGTa                                                                   >  2:1292792/1‑93 (MQ=255)

                            tctttGGTTTCCCATACCGCAAAGAGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCGCTGGGCTATCGCAGGCAAAAGATAGTAAt                                                                 <  1:1428909/89‑1 (MQ=255)

                              tctGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAATgg                                                               >  2:1826816/1‑93 (MQ=255)

                               ctGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCGCTGGGCTATCGCAGGCAAAAGATAGTAATGGa                                                              <  2:7095692/93‑1 (MQ=255)

                               ctGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCGCTGGGCTATCGCAGGCAAAAGATAGTAATGGa                                                              >  1:2159806/1‑93 (MQ=255)

                                tGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCGCTGGGCTATCGCAGGCAAAAGATAGTAATGGaa                                                             >  2:265168/1‑93 (MQ=255)

                                tGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCGCTGGGCTATCGCAGGCAAAAGATAGTAATGGaa                                                             >  1:6369231/1‑93 (MQ=255)

                                tGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAATGGaa                                                             <  2:2443396/93‑1 (MQ=255)

                                   tttCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCGCTGGGCTATCGCAGGCAAAAGATAGTAATGGAACaa                                                          >  1:7497359/1‑93 (MQ=255)

                                   tttCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCGCTGGGCTATCGCAGGCAAAAGATAGTAATGGAACaa                                                          >  2:4907446/1‑93 (MQ=255)

                                   tttCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCGCTGGGCTATCGCAGGCAAAAGATAGTAATGGAACaa                                                          >  1:7005389/1‑93 (MQ=255)

                                     tCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAATGGAACaaaa                                                        <  2:4586278/93‑1 (MQ=255)

                                       ccATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCGCTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAgg                                                      >  1:855258/1‑93 (MQ=255)

                                        cATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCGCTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGc                                                     <  2:1213683/93‑1 (MQ=255)

                                        cATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCGCTAGGCTATCGCAGGCAACAGATAGTAATGGAACAAAAGGc                                                     >  2:6990782/1‑93 (MQ=255)

                                          tACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCGCTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTa                                                   >  2:668044/1‑93 (MQ=255)

                                          tACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTa                                                   >  2:2333285/1‑93 (MQ=255)

                                           aCCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTAt                                                  <  2:6796126/93‑1 (MQ=255)

                                            ccGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCGCTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATg                                                 <  2:5841291/93‑1 (MQ=255)

                                            ccGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCGCTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATg                                                 <  1:2544242/93‑1 (MQ=255)

                                            ccGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCGCTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATg                                                 >  2:3583446/1‑93 (MQ=255)

                                             cGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGt                                                <  1:5672898/93‑1 (MQ=255)

                                              gCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCGCTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGtt                                               >  1:5509865/1‑93 (MQ=255)

                                              gCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCGCTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGtt                                               <  2:1636289/93‑1 (MQ=255)

                                              gCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCGCTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGtt                                               >  1:5747835/1‑93 (MQ=255)

                                              gCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGtt                                               >  1:5473200/1‑93 (MQ=255)

                                                aaaGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCGCTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGtttt                                             >  2:955868/1‑93 (MQ=255)

                                                aaaGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGtttt                                             >  1:7339671/1‑93 (MQ=255)

                                                 aaGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCGCTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGttttt                                            >  2:3799565/1‑93 (MQ=255)

                                                 aaGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCGCTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGttttt                                            >  1:23621/1‑93 (MQ=255)

                                                  aGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTg                                           <  2:2229405/93‑1 (MQ=255)

                                                    ggTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCGCTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTa                                         >  2:5515959/1‑93 (MQ=255)

                                                    ggTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTa                                         >  1:4581992/1‑93 (MQ=255)

                                                     gTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCGCTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAg                                        >  2:5230889/1‑93 (MQ=255)

                                                      tAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCGCTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAgg                                       >  2:3229109/1‑93 (MQ=255)

                                                      tAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCGCTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAgg                                       >  1:3711712/1‑93 (MQ=255)

                                                       aGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAggg                                      >  1:2808368/1‑93 (MQ=255)

                                                        gTTCAGGAAAGTATCAATCGCAGTCAGACACCATCGCTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAgggg                                     >  2:7238122/1‑93 (MQ=255)

                                                        gTTCAGGAAAGTATCAATCGCAGTCAGACACCATCGCTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAgggg                                     <  2:5548210/93‑1 (MQ=255)

                                                         ttCAGGAAAGTATCAATCGCAGTCAGACACCATCGCTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGa                                    >  1:3180215/1‑93 (MQ=255)

                                                         ttCAGGAAAGTATCAATCGCAGTCAGACACCATCGCTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGa                                    >  2:3049455/1‑93 (MQ=255)

                                                         ttCAGGAAAGTATCAATCGCAGTCAGACACCATCGCTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGa                                    >  1:4370370/1‑93 (MQ=255)

                                                          tCAGGAAAGTATCAATCGCAGTCAGACACCATCGCTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGaa                                   >  2:5911302/1‑93 (MQ=255)

                                                            aGGAAAGTATCAATCGCAGTCAGACACCATCGCTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATc                                 <  2:673749/93‑1 (MQ=255)

                                                            aGGAAAGTATCAATCGCAGTCAGACACCATCGCTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATc                                 >  1:1124559/1‑93 (MQ=255)

                                                             ggAAAGTATCAATCGCAGTCAGACACCATCGCTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCg                                >  1:134598/1‑93 (MQ=255)

                                                             ggAAAGTATCAATCGCAGTCAGACACCATCGCTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCg                                >  2:7437239/1‑93 (MQ=255)

                                                              gAAAGTATCAATCGCAGTCAGACACCATCGCTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGc                               <  2:6478576/93‑1 (MQ=255)

                                                                aaGTATCAATCGCAGTCAGACACCATCGCTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTg                             <  1:7145052/93‑1 (MQ=255)

                                                                 aGTATCAATCGCAGTCAGACACCATCGCTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTgg                            >  1:1774201/1‑93 (MQ=255)

                                                                 aGTATCAATCGCAGTCAGACACCATCGCTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTgg                            >  2:1136884/1‑93 (MQ=255)

                                                                  gTATCAATCGCAGTCAGACACCATCGCTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTGGa                           <  2:2040489/93‑1 (MQ=255)

                                                                   tATCAATCGCAGTCAGACACCATCGCTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTGGAt                          <  2:3346912/93‑1 (MQ=255)

                                                                   tATCAATCGCAGTCAGACACCATCGCTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTGGAt                          <  1:4434197/93‑1 (MQ=255)

                                                                       aaTCGCAGTCAGACACCATCGCTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTGGATTAcc                      <  1:1015618/93‑1 (MQ=255)

                                                                       aaTCGCAGTCAGACACCATCGCTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTGGATTAcc                      >  2:6321856/1‑93 (MQ=255)

                                                                        aTCGCAGTCAGACACCATCGCTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTGGATTACCt                     <  1:6614750/93‑1 (MQ=255)

                                                                        aTCGCAGTCAGACACCATCGCTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTGGATTACCt                     <  1:2064586/93‑1 (MQ=255)

                                                                         tCGCAGTCAGACACCATCGCTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTGGATTACCtt                    >  1:5875652/1‑93 (MQ=255)

                                                                         tCGCAGTCAGACACCATCGCTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTGGATTACCtt                    <  2:2808050/93‑1 (MQ=255)

                                                                           gCAGTCAGACACCATCGCTGGGCTATCGCAGGCAAAAGAGAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTGGATTACCTTAt                  >  1:6219519/1‑93 (MQ=255)

                                                                             aGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTGGATTACCTTATCa                >  1:6057180/1‑93 (MQ=255)

                                                                                 aGACACCATCGCTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTGGATTACCTTATCACTGa            <  1:3797999/93‑1 (MQ=255)

                                                                                 aGACACCATCGCTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTGGATTACCTTATCACTGa            <  2:558804/93‑1 (MQ=255)

                                                                                  gACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTGGATTACCTTATCACTGAt           >  2:576212/1‑93 (MQ=255)

                                                                                    cacCATCGCTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTGGATTACCTTATCACTGATgg         <  2:685271/93‑1 (MQ=255)

                                                                                       cATCGCTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTGGATTACCTTATCACTGATGGTGc      <  2:6656153/93‑1 (MQ=255)

                                                                                        aTCGCTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTGGATTACCTTATCACTGATGGTGcc     >  1:3479416/1‑93 (MQ=255)

                                                                                        aTCACTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTGGATTACCTTATCACTGATGGTGcc     <  1:2611726/93‑1 (MQ=255)

GCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTGGATTACCTTATCACTGATGGTGCCGAT  >  NC_000913/3873118‑3873301

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

GCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTGGATTACCTTATCACTGATGGTGCCGATG  >  NC_000913/3873116‑3873302

GCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCG                                                                                               <  SRR4302542.1331412/94‑1 (MQ=60)

 CGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCGC                                                                                              >  SRR4302542.6507416/1‑94 (MQ=60)

 CGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCAC                                                                                              >  SRR4302542.4899220/1‑94 (MQ=60)

  GCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCGCT                                                                                             <  SRR4302542.7159122/94‑1 (MQ=60)

  GCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCGCT                                                                                             >  SRR4302542.1702111/1‑94 (MQ=60)

  GCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCGCT                                                                                             <  SRR4302542.4218936/94‑1 (MQ=60)

  GCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCGCT                                                                                             <  SRR4302542.6077777/94‑1 (MQ=60)

  GATGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATTGCAGTCAGACACCATCGCT                                                                                             <  SRR4302542.6517625/94‑1 (MQ=60)

   CTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCGCTG                                                                                            >  SRR4302542.3751499/1‑94 (MQ=60)

    TGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGG                                                                                           <  SRR4302542.6663415/94‑1 (MQ=60)

     GCTGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTGCAGGAAAGTAGCAATCGCAGTCAGACACCACCGCTGGG                                                                                          <  SRR4302542.3431165/94‑1 (MQ=60)

     GATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCGCTGGG                                                                                          >  SRR4302542.2677449/1‑94 (MQ=60)

     GATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCGCTGGG                                                                                          <  SRR4302542.6827416/94‑1 (MQ=60)

     GATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCGCTGGG                                                                                          <  SRR4302542.1940780/94‑1 (MQ=60)

     GATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCGCTGGG                                                                                          >  SRR4302542.4118601/1‑94 (MQ=60)

     GATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGG                                                                                          <  SRR4302542.1422710/94‑1 (MQ=60)

     GATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGG                                                                                          <  SRR4302542.7233733/94‑1 (MQ=60)

        GCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCGCTGGGCTA                                                                                       <  SRR4302542.6540031/94‑1 (MQ=60)

        GCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCGCTGGGCTA                                                                                       <  SRR4302542.2531063/94‑1 (MQ=60)

         CTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCGCTGGGCTAT                                                                                      >  SRR4302542.95670/1‑94 (MQ=60)

           TTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCGCTGGGCTATCG                                                                                    <  SRR4302542.4015076/94‑1 (MQ=60)

           TTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCGCTGGGCTATCG                                                                                    <  SRR4302542.6811440/94‑1 (MQ=60)

           TTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCGCTGGGCTATCG                                                                                    >  SRR4302542.78201/1‑94 (MQ=60)

             CAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCATTCGCAGTCAGACACCATCGCTGGGCTATCGCA                                                                                  <  SRR4302542.4531370/94‑1 (MQ=60)

             CAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCGCTGGGCTATCGCA                                                                                  >  SRR4302542.2917887/1‑94 (MQ=60)

              AGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCGCTGGGCTATCGCAG                                                                                 >  SRR4302542.4926692/1‑94 (MQ=60)

              AGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCGCTGGGCTATCGCAG                                                                                 <  SRR4302542.1516693/94‑1 (MQ=60)

               GGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCGCTGGGCTATCGCAGG                                                                                <  SRR4302542.7536306/94‑1 (MQ=60)

               GGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCGCTGGGCTATCGCAGG                                                                                <  SRR4302542.2172325/94‑1 (MQ=60)

               GGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATCGCAGG                                                                                <  SRR4302542.3345397/94‑1 (MQ=60)

                 CTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCGCTGGGCTATCGCAGGCA                                                                              >  SRR4302542.1754981/1‑94 (MQ=60)

                   GTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCGCTGGGCTATCGCAGGCAAA                                                                            >  SRR4302542.4757110/1‑94 (MQ=60)

                    TATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCGCTGGGCTATCGCAGGCAAAA                                                                           >  SRR4302542.4042576/1‑94 (MQ=60)

                     ATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCGCTGGGCTATCGCAGGCAAAAG                                                                          <  SRR4302542.4822786/94‑1 (MQ=60)

                      TCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCGCTGGGCTATCGCAGGCAAAAGA                                                                         <  SRR4302542.3073342/94‑1 (MQ=60)

                       CTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCGCTGGGCTATCGCAGGCAAAAGAT                                                                        <  SRR4302542.3763194/94‑1 (MQ=60)

                       CTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCGCTGGGCTATCGCAGGCAAAAGAT                                                                        >  SRR4302542.3564410/1‑94 (MQ=60)

                        TCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCGCTGGGCTATCGCAGGCAAAAGATA                                                                       <  SRR4302542.6505870/94‑1 (MQ=60)

                         CGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCGCTGGGCTATCGCAGGCAAAAGATAG                                                                      >  SRR4302542.5540281/1‑94 (MQ=60)

                         CGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCGCTGGGCTATCGCAGGCAAAAGATAG                                                                      >  SRR4302542.136015/1‑94 (MQ=60)

                         CGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCGCTGGGCTATCGCAGGCAAAAGATAG                                                                      <  SRR4302542.5316031/94‑1 (MQ=60)

                         CGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCGCTGGGCTATCGCAGGCAAAAGATAG                                                                      <  SRR4302542.538023/94‑1 (MQ=60)

                          GTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCGCTGGGCTATCGCAGGCAAAAGATAGT                                                                     >  SRR4302542.1373206/1‑94 (MQ=60)

                          GTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCGCTGGGCTATCGCAGGCAAAAGATAGT                                                                     >  SRR4302542.122795/1‑94 (MQ=60)

                          GTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCGCTGGGCTATCGCAGGCAAAAGATAGT                                                                     >  SRR4302542.1933462/1‑94 (MQ=60)

                           TATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCGGACACCATCGCTGGGCTATCGCAGGCAAAAGATAGTA                                                                    >  SRR4302542.7121870/1‑94 (MQ=60)

                           TATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCGCTGGGCTATCGCAGGCAAAAGATAGTA                                                                    >  SRR4302542.6872327/1‑94 (MQ=60)

                             TGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCGCTGGGCTATCGCAGGCAAAAGATAGTAAT                                                                  <  SRR4302542.5796839/94‑1 (MQ=60)

                             TGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCGCTGGGCTATCGCAGGCAAAAGATAGTAAT                                                                  <  SRR4302542.3072100/94‑1 (MQ=60)

                             TGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCGCTGGGCTATCGCAGGCAAAAGATAGTAAT                                                                  <  SRR4302542.441005/94‑1 (MQ=60)

                              tcttTGGTTTCCCATACCGCAAAGAGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCGCTGGGCTATCGCAGGCAAAAGATAGTAATG                                                                 <  SRR4302542.1441269/90‑1 (MQ=60)

                              GCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCGCTGGGCTATCGCAGGCAAAAGATAGTAATG                                                                 >  SRR4302542.1376139/1‑94 (MQ=60)

                              GCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCGCTGGGCTATCGCAGGCAAAAGATAGTAATG                                                                 <  SRR4302542.4339138/94‑1 (MQ=60)

                                TCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCGCTGGGCTATCGCAGGCAAAAGATAGTAATGGA                                                               >  SRR4302542.2178408/1‑94 (MQ=60)

                                 CTTGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAATGGAA                                                              <  SRR4302542.6290120/94‑1 (MQ=60)

                                 CTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCGCTGGGCTATCGCAGGCAAAAGATAGTAATGGAA                                                              >  SRR4302542.6964684/1‑94 (MQ=60)

                                 CTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCGCTGGGCTATCGCAGGCAAAAGATAGTAATGGAA                                                              >  SRR4302542.6431952/1‑94 (MQ=60)

                                 CTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCGCTGGGCTATCGCAGGCAAAAGATAGTAATGGAA                                                              >  SRR4302542.2313554/1‑94 (MQ=60)

                                 CTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAATGGAA                                                              >  SRR4302542.3707437/1‑94 (MQ=60)

                                 CTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAATGGAA                                                              >  SRR4302542.7105823/1‑94 (MQ=60)

                                  TGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCGCTGGGCTATCGCAGGCAAAAGATAGTAATGGAAC                                                             >  SRR4302542.3250428/1‑94 (MQ=60)

                                    GTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCGCTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAA                                                           >  SRR4302542.1504770/1‑94 (MQ=60)

                                    GTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCGCTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAA                                                           >  SRR4302542.7571039/1‑94 (MQ=60)

                                    GTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCGCTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAA                                                           >  SRR4302542.7074206/1‑94 (MQ=60)

                                      TTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCGCTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAA                                                         >  SRR4302542.739517/1‑94 (MQ=60)

                                       TCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCGCTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAG                                                        <  SRR4302542.461051/94‑1 (MQ=60)

                                       TCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCGCTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAG                                                        <  SRR4302542.2256366/94‑1 (MQ=60)

                                       TCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCGCTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAG                                                        <  SRR4302542.4909590/94‑1 (MQ=60)

                                        CCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCGCTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGG                                                       >  SRR4302542.862631/1‑94 (MQ=60)

                                          CATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCGCTGGGCTATCGCAGGCAAAAGATAGTAATGGACCAAAAGGCT                                                     >  SRR4302542.6224925/1‑94 (MQ=60)

                                          CATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCGCTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCT                                                     <  SRR4302542.6783173/94‑1 (MQ=60)

                                          CATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCGCTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCT                                                     >  SRR4302542.6224924/1‑94 (MQ=60)

                                           ATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCGCTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTA                                                    >  SRR4302542.3195409/1‑94 (MQ=60)

                                           ATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCGCTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTA                                                    >  SRR4302542.1298303/1‑94 (MQ=60)

                                            TACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCGCTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTAT                                                   <  SRR4302542.2031987/94‑1 (MQ=60)

                                             ccgGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATG                                                  <  SRR4302542.3750963/91‑1 (MQ=60)

                                              CCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCGCTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGT                                                 <  SRR4302542.2568902/94‑1 (MQ=60)

                                               CGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCGCTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTT                                                >  SRR4302542.4154824/1‑94 (MQ=60)

                                               CGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCGCTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTT                                                >  SRR4302542.5563409/1‑94 (MQ=60)

                                               CGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCGCTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTT                                                >  SRR4302542.1597799/1‑94 (MQ=60)

                                               CGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCGCTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTT                                                >  SRR4302542.5803629/1‑94 (MQ=60)

                                               CGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTT                                                <  SRR4302542.5727994/94‑1 (MQ=60)

                                               CGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTT                                                >  SRR4302542.5526372/1‑94 (MQ=60)

                                                GCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCGCTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTT                                               <  SRR4302542.6020752/94‑1 (MQ=60)

                                                GCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCGCTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTT                                               >  SRR4302542.7155548/1‑94 (MQ=60)

                                                GCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTT                                               >  SRR4302542.4835385/1‑94 (MQ=60)

                                                 CAAAGGGTGGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCGCTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTT                                              >  SRR4302542.1187129/1‑94 (MQ=60)

                                                 CAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTT                                              >  SRR4302542.7411564/1‑94 (MQ=60)

                                                  AAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCGCTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTT                                             >  SRR4302542.23824/1‑94 (MQ=60)

                                                   AAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCGCTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTG                                            <  SRR4302542.5518164/94‑1 (MQ=60)

                                                    AGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCGCTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGT                                           <  SRR4302542.6455325/94‑1 (MQ=60)

                                                     GGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTA                                          >  SRR4302542.4626819/1‑94 (MQ=60)

                                                      GGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCGCTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAG                                         <  SRR4302542.4433844/94‑1 (MQ=60)

                                                      GGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCGCTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAG                                         <  SRR4302542.5784087/94‑1 (MQ=60)

                                                       GTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCGCTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGG                                        >  SRR4302542.3748430/1‑94 (MQ=60)

                                                        TAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCGCTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGG                                       >  SRR4302542.5535219/1‑94 (MQ=60)

                                                        TAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGG                                       >  SRR4302542.2835406/1‑94 (MQ=60)

                                                         AGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCGCTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGG                                      <  SRR4302542.6361828/94‑1 (MQ=60)

                                                          GTTCAGGAAAGTATCAATCGCAGTCAGACACCATCGCTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGA                                     >  SRR4302542.4413278/1‑94 (MQ=60)

                                                          GTTCAGGAAAGTATCAATCGCAGTCAGACACCATCGCTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGA                                     >  SRR4302542.3210350/1‑94 (MQ=60)

                                                          GTTCAGGAAAGTATCAATCGCAGTCAGACACCATCGCTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGA                                     <  SRR4302542.2605513/94‑1 (MQ=60)

                                                            TCAGGAAAGTATCAATCGCAGTCAGACACCATCGCTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAAT                                   <  SRR4302542.3097432/94‑1 (MQ=60)

                                                            TCAGGAAAGTATCAATCGCAGTCAGACACCATCGCTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAAT                                   <  SRR4302542.5850077/94‑1 (MQ=60)

                                                             CAGGAAAGTATCAATCGCAGTCAGACACCATCGCTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATC                                  >  SRR4302542.974972/1‑94 (MQ=60)

                                                             CAGGAAAGTATCAATCGCAGTCAGACACCATCGCTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATC                                  >  SRR4302542.1134165/1‑94 (MQ=60)

                                                             CAGGAAAGTATCAATCGCAGTCAGACACCATCGCTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATC                                  >  SRR4302542.1134166/1‑94 (MQ=60)

                                                             CAGGAAAGTATCAATCGCAGTCAGACACCATCGCTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATC                                  >  SRR4302542.3774284/1‑94 (MQ=60)

                                                              AGGAAAGTATCAATCGCAGTCAGACACCATCGCTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCG                                 >  SRR4302542.6659151/1‑94 (MQ=60)

                                                              AGGAAAGTATCAATCGCAGTCAGACACCATCGCTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCG                                 >  SRR4302542.135684/1‑94 (MQ=60)

                                                              AGGAAAGTATCAATCGCAGTCAGACACCATCGCTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCG                                 >  SRR4302542.3541693/1‑94 (MQ=60)

                                                               GGAAAGTATCAATCGCAGTCAGACACCATCGCTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGC                                >  SRR4302542.2801241/1‑94 (MQ=60)

                                                                GAAAGTATCAATCGCAGTCAGACACCATCGCTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCT                               <  SRR4302542.3553817/94‑1 (MQ=60)

                                                                GAAAGTATCAATCGCAGTCAGACACCATCGCTGGGCTATCGCAGGCAAAAGATAGTAAGGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCT                               >  SRR4302542.1069843/1‑94 (MQ=60)

                                                                  AAGTATCAATCGCAGTCAGACACCATCGCTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTGG                             >  SRR4302542.1098576/1‑94 (MQ=60)

                                                                  AAGTATCAATCGCAGTCAGACACCATCGCTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTGG                             <  SRR4302542.582699/94‑1 (MQ=60)

                                                                  AAGTATCAATCGCAGTCAGACACCATCGCTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTGG                             <  SRR4302542.7215162/94‑1 (MQ=60)

                                                                  AAGTATCAATCGCAGTCAGACACCATCGCTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTGG                             >  SRR4302542.1789579/1‑94 (MQ=60)

                                                                  AAGTATCAATCGCAGTCAGACACCATCGCTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTGG                             >  SRR4302542.3613247/1‑94 (MQ=60)

                                                                   AGTATCAATCGCAGTCAGACACCATCGCTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTGGA                            >  SRR4302542.2469061/1‑94 (MQ=60)

                                                                    GTATCAATCGCAGTCAGACACCATCGCTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTGGAT                           <  SRR4302542.1619317/94‑1 (MQ=60)

                                                                    GTATCAATCGCAGTCAGACACCATCGCTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTGGAT                           <  SRR4302542.21596/94‑1 (MQ=60)

                                                                    GTATCAATCGCAGTCAGACACCATCGCTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTGGAT                           >  SRR4302542.4756778/1‑94 (MQ=60)

                                                                     TATCAATCGCAGTCAGACACCATCGCTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTGGATT                          <  SRR4302542.4477726/94‑1 (MQ=60)

                                                                        CAATCGCAGTCAGACACCATCGCTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTGGATTACC                       >  SRR4302542.5700514/1‑94 (MQ=60)

                                                                         AATCGCAGTCAGACACCATCGCTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTGGATTACCT                      >  SRR4302542.2696433/1‑94 (MQ=60)

                                                                         AATCGCAGTCAGACACCATCGCTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTGGATTACCT                      <  SRR4302542.1024365/94‑1 (MQ=60)

                                                                          ATCGCAGTCAGACACCATCGCTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTGGATTACCTT                     <  SRR4302542.6679962/94‑1 (MQ=60)

                                                                          ATCGCAGTCAGACACCATCGCTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTGGATTACCTT                     >  SRR4302542.5932610/1‑94 (MQ=60)

                                                                          ATCGCAGTCAGACACCATCGCTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTGGATTACCTT                     <  SRR4302542.2082434/94‑1 (MQ=60)

                                                                           TCGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTGGATTACCTTA                    <  SRR4302542.257177/94‑1 (MQ=60)

                                                                            CGCAGTCAGACACCATCGCTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTGGATTACCTTAT                   >  SRR4302542.5996481/1‑94 (MQ=60)

                                                                            CGCAGTCAGACACCATCGCTGGGCTATCGCAGGCAAAAGAGAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTGGATTACCTTAT                   >  SRR4302542.6280774/1‑94 (MQ=60)

                                                                              CAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTGGATTACCTTATCA                 >  SRR4302542.6115810/1‑94 (MQ=60)

                                                                               AGTCAGACACCATCGCTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTGGATTACCTTATCAC                >  SRR4302542.348381/1‑94 (MQ=60)

                                                                               AGTCAGACACCATCGCTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTGGATTACCTTATCAC                >  SRR4302542.5653920/1‑94 (MQ=60)

                                                                               AGTCAGACACCATCGCTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTGGATTACCTTATCAC                >  SRR4302542.7505284/1‑94 (MQ=60)

                                                                               AGTCAGACACCATCGCTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTGGATTACCTTATCAC                <  SRR4302542.1485024/94‑1 (MQ=60)

                                                                                GTCAGACACCATCGCTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTGGATTACCTTATCACT               >  SRR4302542.1281968/1‑94 (MQ=60)

                                                                                  CAGACACCATCGCTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTGGATTACCTTATCACTGA             >  SRR4302542.1359752/1‑94 (MQ=60)

                                                                                  CAGACACCATCGCTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGCGAATCGCTGGATTACCTTATCACTGA             >  SRR4302542.7603219/1‑94 (MQ=60)

                                                                                   AGACACCATCGCTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTGGATTACCTTATCACTGAT            <  SRR4302542.3835597/94‑1 (MQ=60)

                                                                                      CACCATCGCTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTGGATTACCTTATCACTGATGGT         >  SRR4302542.3823000/1‑94 (MQ=60)

                                                                                       ACCATCGCTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTGGATTACCTTATCACTGATGGTG        >  SRR4302542.3340741/1‑94 (MQ=60)

                                                                                       ACCATCACTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTGGATTACCTTATCACTGATGGTG        >  SRR4302542.1739054/1‑94 (MQ=60)

                                                                                       ACCATCACTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTGGATTACCTTATCACTGATGGTG        <  SRR4302542.1907032/94‑1 (MQ=60)

                                                                                        CCATCGCTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTGGATTACCTTATCACTGATGGTGC       >  SRR4302542.468066/1‑94 (MQ=60)

                                                                                        CCATCGCTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTGGATTACCTTATCACTGATGGTGC       >  SRR4302542.2036844/1‑94 (MQ=60)

                                                                                         CATCGCTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTGGATTACCTTATCACTGATGGTGCC      >  SRR4302542.3511979/1‑94 (MQ=60)

                                                                                         CATCGCTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTGGATTACCTTATCACTGATGGTGCC      >  SRR4302542.4875902/1‑94 (MQ=60)

                                                                                          ATCACTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTGGATTACCTTATCACTGATGGTGCCA     <  SRR4302542.2636972/94‑1 (MQ=60)

                                                                                             GCTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTGGATTACCTTATCACTGATGGTGCCGATG  >  SRR4302542.5029810/1‑94 (MQ=60)

GCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTGGATTACCTTATCACTGATGGTGCCGATG  >  NC_000913/3873116‑3873302

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`