Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1061 294.1 15065038 98.6% 14854127 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,929,607 T→C V222A (GTG→GCG)  kup → low affinity potassium transport system

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,929,6070TC87.5% 703.1 / 81.0 281V222A (GTG→GCG) kuplow affinity potassium transport system
Reads supporting (aligned to +/- strand):  ref base T (19/16);  new base C (129/117);  total (148/133)
Fisher's exact test for biased strand distribution p-value = 8.59e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.64e-01

Only 100 of 305 total aligned reads displayed.

CGGAAGTGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTAC‑GGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACCGTCGTATTA  >  NC_000913/3929517‑3929698
                                                                                          |                                                                                            
   aaGCGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGcgttgt                                                                                         <  1:6728191/93‑1 (MQ=255)
    agcgctgcATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGCGTTGTc                                                                                        >  1:3183282/4‑93 (MQ=255)
    agcgctgcATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGCGTTGTc                                                                                        <  2:3079988/90‑1 (MQ=255)
     gcgctgcATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGCGTTGTCg                                                                                       >  2:4616733/3‑93 (MQ=255)
     gcgctgcATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGCGTTGTCg                                                                                       >  1:4664479/3‑93 (MQ=255)
      cgctgcATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGCGTTGTCGa                                                                                      <  1:3047471/92‑1 (MQ=255)
        ctgcATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCATTGGCGTTGTCGAtt                                                                                    <  2:6440215/93‑1 (MQ=255)
         tgcATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGCGTTGTCGATTa                                                                                   >  2:3590796/1‑93 (MQ=255)
            aTGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGCGTTGTCGATTAC‑gg                                                                               <  2:1887791/93‑1 (MQ=255)
             tGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGCGTTGTCGATTAC‑ggg                                                                              >  1:4090753/1‑93 (MQ=255)
             tGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGCGTTGTCGATTAC‑ggg                                                                              <  1:5858886/93‑1 (MQ=255)
             tGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGCGTTGTCGATTAC‑ggg                                                                              <  2:407500/93‑1 (MQ=255)
              gcgcTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGCGTTGTCGATTAC‑gggg                                                                             <  2:7107370/93‑1 (MQ=255)
               cgcTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGCGTTGTCGATTAC‑ggggg                                                                            <  1:4292074/93‑1 (MQ=255)
               cgcTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGCGTTGTCGATTAC‑ggggg                                                                            <  1:3345580/93‑1 (MQ=255)
                gcTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGCGTTGTCGATTAC‑GGGGGt                                                                           >  2:2271100/1‑93 (MQ=255)
                 cTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGCGTTGTCGATTAC‑GGGGGTc                                                                          <  2:6327737/93‑1 (MQ=255)
                   gAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGCGTTGTCGATTAC‑GGGGGTCGa                                                                        <  1:1346992/93‑1 (MQ=255)
                    aaTCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGCGTTGTCGATTAC‑GGGGGTCGAg                                                                       <  2:6261490/93‑1 (MQ=255)
                     aTCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGCGTTGTCGATTAC‑GGGGGTCGAgg                                                                      <  2:4167438/93‑1 (MQ=255)
                      tCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGCGTTGTCGATTAC‑GGGGGTCGAGgc                                                                     >  2:4901425/1‑93 (MQ=255)
                       ccAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTAC‑GGGGGTCGAggtg                                                                    <  2:3304507/93‑3 (MQ=255)
                          aTGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGCGTTGTCGATTAC‑GGGGGTCGAGGCGCTg                                                                 >  1:571605/1‑93 (MQ=255)
                          aTGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGCGTTGTCGATTAC‑GGGGGTCGAGGCGCTg                                                                 >  2:4335596/1‑93 (MQ=255)
                          aTGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGCGTTGTCGATTAC‑GGGGGTCGAGGCGCTg                                                                 <  2:698306/93‑1 (MQ=255)
                            gtgGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGCGTTGTCGATTAC‑GGGGGTCGAGGCGCTGTa                                                               <  1:7255218/93‑1 (MQ=255)
                             tgGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGCGTTGTCGATTAC‑GGGGGTCGAGGCGCTGTAt                                                              >  1:4006186/1‑93 (MQ=255)
                             tgGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGCGTTGTCGATTAC‑GGGGGTCGAGGCGCTGTAt                                                              <  1:1109525/93‑1 (MQ=255)
                              gggCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGCGTTGTCGATTAC‑GGGGGTCGAGGCGCTGTATg                                                             >  1:2961761/1‑93 (MQ=255)
                              gggCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGCGTTGTCGATTAC‑GGGGGTCGAGGCGCTGTATg                                                             <  1:1795320/93‑1 (MQ=255)
                               ggCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGCGTTGTCGATTAC‑GGGGGTCGAGGCGCTGTATGc                                                            <  1:3639913/93‑1 (MQ=255)
                                  ggTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTAC‑GGGGGTCGAGGTGCTGTATGCTGa                                                         >  1:6822978/1‑93 (MQ=255)
                                    tGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGCGTTGTCGATTAC‑GGGGGTCGAGGCGCTGTATGCTGata                                                       >  2:7399621/1‑93 (MQ=255)
                                    tGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGCGTTGTCGATTAC‑GGGGGTCGAGGCGCTGTATGCTGata                                                       <  2:4411192/93‑1 (MQ=255)
                                    tGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGCGTTGTCGATTAC‑GGGGGTCGAGGCGCTGTATGCTGata                                                       <  2:2699929/93‑1 (MQ=255)
                                    tGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGCGTTGTCGATTAC‑GGGGGTCGAGGCGCTGTATGCTGata                                                       >  2:2355323/1‑93 (MQ=255)
                                    tGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGCGTTGTCGATTAC‑GGGGGTCGAGGCGCTGTATGCTGata                                                       <  2:4467196/93‑1 (MQ=255)
                                    tGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGCGTTGTCGATTAC‑GGGGGTCGAGGCGCTGTATGCTGata                                                       >  2:5336435/1‑93 (MQ=255)
                                     gCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGCGTTGTCGATTAC‑GGGGGTCGAGGCGCTGTATGCTGatat                                                      >  1:1005571/1‑93 (MQ=255)
                                     gCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGCGTTGTCGATTAC‑GGGGGTCGAGGCGCTGTATGCTGatat                                                      <  2:5967291/93‑1 (MQ=255)
                                     gCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGCGTTGTCGATTAC‑GGGGGTCGAGGCGCTGTATGCTGatat                                                      >  1:6963313/1‑93 (MQ=255)
                                      cATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTAC‑GGGGGTCGAGGTGCTGTATGCTGATATg                                                     <  1:1636809/93‑1 (MQ=255)
                                      cATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGCGTTGTCGATTAC‑GGGGGTCGAGGCGCTGTATGCTGATATg                                                     <  2:6527171/93‑1 (MQ=255)
                                       aTTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGCGTTGTCGATTAC‑GGGGGTCGAGGCGCTGTATGCTGATATgg                                                    >  2:6754932/1‑93 (MQ=255)
                                       aTTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGCGTTGTCGATTAC‑GGGGGTCGAGGCGCTGTATGCTGATATgg                                                    >  1:4203111/1‑93 (MQ=255)
                                        tttCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGCGTTGTCGATTAC‑GGGGGTCGAGGCGCTGTATGCTGATATggg                                                   <  2:509626/93‑1 (MQ=255)
                                        tttCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGCGTTGTCGATTAC‑GGGGGTCGAGGCGCTGTATGCTGATATggg                                                   >  2:1621083/1‑93 (MQ=255)
                                        tttCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGCGTTGTCGATTAC‑GGGGGTCGAGGCGCTGTATGCTGATATggg                                                   >  1:2676789/1‑93 (MQ=255)
                                        tttCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGCGTTGTCGATTAC‑GGGGGTCGAGGCGCTGTATGCTGATATggg                                                   <  2:2481123/93‑1 (MQ=255)
                                        tttCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGCGTTGTCGATTAC‑GGGGGTCGAGGCGCTGTATGCTGATATggg                                                   >  1:5017646/1‑93 (MQ=255)
                                          tcttcCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGCGTTGTCGATTAC‑GGGGGTCGAGGCGCTGTATGCTGATATGGGGc                                                 >  2:7277580/1‑93 (MQ=255)
                                          tcttcCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGCGTTGTCGATTAC‑GGGGGTCGAGGCGCTGTATGCTGATATGGGGc                                                 >  2:5242303/1‑93 (MQ=255)
                                          tcttcCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGCGTTGTCGATTAC‑GGGGGTCGAGGCGCTGTATGCTGATATGGGGc                                                 >  2:3813055/1‑93 (MQ=255)
                                           cttcCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGCGTTGTCGATTAC‑GGGGGTCGAGGCGCTGTATGCTGATATGGGGCa                                                >  1:1475360/1‑93 (MQ=255)
                                           cttcCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGCGTTGTCGATTAC‑GGGGGTCGAGGCGCTGTATGCTGATATGGGGCa                                                >  1:4600387/1‑93 (MQ=255)
                                            ttcCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGCGTTGTCGATTAC‑GGGGGTCGAGGCGCTGTATGCTGATATGGGGCAc                                               >  1:5592355/1‑93 (MQ=255)
                                              ccTTGAATACAAAACGGTTTTTTTTATTGCATTAGGGGCAGTGGTGATGTCGATTAC‑GGGGGACGAGGAGCTGTATGCTGATATGGGGCACtt                                             >  2:4290850/1‑93 (MQ=255)
                                              ccTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGCGTTGTCGATTAC‑GGGGGTCGAGGCGCTGTATGCTGATATGGGGCACtt                                             <  2:4706506/93‑1 (MQ=255)
                                              ccTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGCGTTGTCGATTAC‑GGGGGTCGAGGCGCTGTATGCTGATATGGGGCACtt                                             >  1:2411624/1‑93 (MQ=255)
                                                   aaTACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTAC‑GGGGGTCGAGGTGCTGTATGCTGATATGGCGCACTTTGGTa                                        <  1:2534343/93‑1 (MQ=255)
                                                   aaTACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGCGTTGTCGATTAC‑GGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTa                                        >  1:7367526/1‑93 (MQ=255)
                                                    aTACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTAC‑GGGGGTCGAGGTGCTGTATGCTGATATGGGGCACTTTGGTaa                                       >  2:1217294/1‑93 (MQ=255)
                                                    aTACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGCGTTGTCGATTAC‑GGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTaa                                       <  1:4845438/93‑1 (MQ=255)
                                                    aTACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGCGTTGTCGATTAC‑GGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTaa                                       >  2:2126857/1‑93 (MQ=255)
                                                     tACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGCGTTGTCGATTAC‑GGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAg                                      >  1:6044218/1‑93 (MQ=255)
                                                     tACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGCGTTGTCGATTAC‑GGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAg                                      >  2:7180581/1‑93 (MQ=255)
                                                      aCAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGCGTTGTCGATTAC‑GGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGt                                     <  2:6589403/93‑1 (MQ=255)
                                                      aCAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGCGTTGTCGATTAC‑GGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGt                                     >  1:5083966/1‑93 (MQ=255)
                                                       cAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGCGTTGTCGATTAC‑GGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGtt                                    <  1:4415619/93‑1 (MQ=255)
                                                        aaaaCGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTAC‑GGGGGTCGAGGTGCTGTATGCTGATATGGGGCACTTTGGTAAGTTc                                   <  2:5858708/93‑1 (MQ=255)
                                                        aaaaCGGTTTCTTTTATTGCATTAGGGGCAGTGGCGTTGTCGATTAC‑GGGGGTCGAGGCGCTGTATGCTGATATGGGACACTTTGGTAAGTTc                                   >  2:1988210/1‑93 (MQ=255)
                                                         aaaCGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTACGGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTc                                   <  1:839616/93‑1 (MQ=255)
                                                         aaaCGGTTTCTTTTATTGCATTAGGGGCAGTGGCGTTGTCGATTAC‑GGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTcc                                  >  2:5004360/1‑93 (MQ=255)
                                                         aaaCGGTTTCTTTTATTGCATTAGGGGCAGTGGCGTTGTCGATTAC‑GGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTcc                                  >  1:6244374/1‑93 (MQ=255)
                                                            cGGTTTCTTTTATTGCATTAGGGGCAGTGGCGTTGTCGATTAC‑GGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTa                               <  2:1720645/93‑1 (MQ=255)
                                                            cGGTTTCTTTTATTGCATTAGGGGCAGTGGCGTTGTCGATTAC‑GGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTa                               <  1:4908957/93‑1 (MQ=255)
                                                            cGGTTTCTTTTATTGCATTAGGGGCAGTGGCGTTGTCGATTAC‑GGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTa                               <  1:1394912/93‑1 (MQ=255)
                                                             ggTTTCTTTTATTGCATTAGGGGCAGTGGCGTTGTCGATTAC‑GGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTAt                              >  1:2860109/1‑93 (MQ=255)
                                                              gTTTCTTTTATTGCATTAGGGGCAGTGGCGTTGTCGATTAC‑GGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTAtt                             <  2:5838520/93‑1 (MQ=255)
                                                               tttCTTTTATTGCATTAGGGGCAGTGGCGTTGTCGATTAC‑GGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTc                            <  2:1522571/93‑1 (MQ=255)
                                                               tttCTTTTATTGCATTAGGGGCAGTGGCGTTGTCGATTAC‑GGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTc                            <  1:6190749/93‑1 (MQ=255)
                                                                  cTTTTATTGCATTAGGGGCAGTGGCGTTGTCGATTAC‑GGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGcc                         <  2:3018405/93‑1 (MQ=255)
                                                                   ttttATTGCATTAGGGGCAGTGGCGTTGTCGATTAC‑GGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCt                        >  2:129364/1‑93 (MQ=255)
                                                                   ttttATTGCATTAGGGGCAGTGGCGTTGTCGATTAC‑GGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCt                        <  2:2052924/93‑1 (MQ=255)
                                                                        ttGCATTAGGGGCAGTGGCGTTGTCGATTAC‑GGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGt                   <  1:1168314/93‑1 (MQ=255)
                                                                        ttGCATTAGGGGCAGTGGCGTTGTCGATTAC‑GGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGt                   >  2:587236/1‑93 (MQ=255)
                                                                          gCATTAGGGGCAGTGGTGTTGTCGATTAC‑GGGGGTCGAGGTGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTgg                 >  2:6822404/1‑93 (MQ=255)
                                                                          gCATTAGGGGCAGTGGCGTTGTCGATTAC‑GGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTgg                 <  2:4802025/93‑1 (MQ=255)
                                                                            aTTAGGGGCAGTGGCGTTGTCGATTAC‑GGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGtt               >  2:3249159/1‑93 (MQ=255)
                                                                             ttAGGGGCAGTGGCGTTGTCGATTAC‑GGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTc              >  2:5485872/1‑93 (MQ=255)
                                                                               aGGGGCAGTGGTGTTGTCGATTAC‑GGGGGTCGAGGTGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCAc            >  1:3767322/1‑93 (MQ=255)
                                                                                 gggCAGTGGCGTTGTCGATTAC‑GGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACcg          >  1:7176441/1‑93 (MQ=255)
                                                                                 gggCAGTGGCGTTGTCGATTAC‑GGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACcg          >  2:1669131/1‑93 (MQ=255)
                                                                                      gtggcgTTGTCGATTAC‑GGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACCGTCGTa     <  1:2004912/93‑1 (MQ=255)
                                                                                      gtggcgTTGTCGATTAC‑GGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACCGTCGTa     >  1:4471097/1‑93 (MQ=255)
                                                                                       tggtgTTGTCGATTAC‑GGGGGTCGAGGTGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACCGTCGTAt    <  1:4795266/93‑1 (MQ=255)
                                                                                       tggcgTTGTCGATTAC‑GGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACCGTCGTAt    >  2:4843885/1‑93 (MQ=255)
                                                                                       tggcgTTGTCGATTAC‑GGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACCGTCGTAt    >  2:5677652/1‑93 (MQ=255)
                                                                                       tggcgTTGTCGATTAC‑GGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACCGTCGTAt    <  1:2762330/93‑1 (MQ=255)
                                                                                         gtgtTGTCGATTAC‑GGGGGTCGAGGTGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACCGTCGTATTa  <  1:5212212/93‑1 (MQ=255)
                                                                                          |                                                                                            
CGGAAGTGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTAC‑GGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACCGTCGTATTA  >  NC_000913/3929517‑3929698

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CCCGGAAGTGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTAC‑GGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACCGTCGTATTACC  >  NC_000913/3929515‑3929700
                                                                                            |                                                                                              
CCCGGAAGCGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGCG                                                                                               >  SRR4302542.1632210/1‑94 (MQ=60)
  CGGAAGCGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGCGTT                                                                                             >  SRR4302542.1679557/1‑94 (MQ=60)
  CGGAAGCGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGCGTT                                                                                             <  SRR4302542.5128384/94‑1 (MQ=60)
   GGAAGCGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGCGTTG                                                                                            >  SRR4302542.914427/1‑94 (MQ=60)
   GGAAGCGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGCGTTG                                                                                            <  SRR4302542.2685946/94‑1 (MQ=60)
     AAGCGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGCGTTGTC                                                                                          >  SRR4302542.3213442/1‑94 (MQ=60)
     AAGCGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGCGTTGTC                                                                                          <  SRR4302542.6794428/94‑1 (MQ=60)
     AAGCGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGCGTTGTC                                                                                          <  SRR4302542.6794425/94‑1 (MQ=60)
      AGCGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGCGTTGTCG                                                                                         >  SRR4302542.4710076/1‑94 (MQ=60)
       GCGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGGTTCTTTTATTGCATTAGGGGCAGTGGCGTTGTCGA                                                                                        >  SRR4302542.1751312/1‑94 (MQ=60)
        CGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGCGTTGTCGAT                                                                                       <  SRR4302542.508912/94‑1 (MQ=60)
        CGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGCGTTGTCGAT                                                                                       <  SRR4302542.3076563/94‑1 (MQ=60)
          CTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTA                                                                                     <  SRR4302542.1169864/94‑1 (MQ=60)
          CTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGCGTTGTCGATTA                                                                                     >  SRR4302542.3006720/1‑94 (MQ=60)
          CTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGCGTTGTCGATTA                                                                                     >  SRR4302542.1361161/1‑94 (MQ=60)
           TGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGCGTTGTCGATTAC                                                                                    >  SRR4302542.6913100/1‑94 (MQ=60)
           TGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGCGTTGTCGATTAC                                                                                    <  SRR4302542.5047396/94‑1 (MQ=60)
           TGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGCGTTGTCGATTAC                                                                                    <  SRR4302542.3120278/94‑1 (MQ=60)
             CATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGCGTTGTCGCTTAC‑GG                                                                                 >  SRR4302542.6885239/1‑94 (MQ=60)
              ATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGGATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGCGTTGTCGATTAC‑GGG                                                                                >  SRR4302542.1839697/1‑94 (MQ=60)
              ATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGCGTTGTCGATTAC‑GGG                                                                                <  SRR4302542.5018259/94‑1 (MQ=60)
              ATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGCGTTGTCGATTAC‑GGG                                                                                <  SRR4302542.1443943/94‑1 (MQ=60)
              ATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGCGTTGTCGATTAC‑GGG                                                                                >  SRR4302542.4131100/1‑94 (MQ=60)
               TGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGCGTTGTCGATTAC‑GGGG                                                                               <  SRR4302542.5915696/94‑1 (MQ=60)
                GCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGCGTTGTCGATTAC‑GGGGG                                                                              <  SRR4302542.46000/94‑1 (MQ=60)
                 CGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGCGTTGTCGATTAC‑GGGGGT                                                                             <  SRR4302542.3377067/94‑1 (MQ=60)
                 CGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGCGTTGTCGATTAC‑GGGGGT                                                                             <  SRR4302542.4334292/94‑1 (MQ=60)
                  GCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGCGTTGTCGATTAC‑GGGGGTC                                                                            <  SRR4302542.3815284/94‑1 (MQ=60)
                   CTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGCGTTGTCGATTAC‑GGGGGTCG                                                                           <  SRR4302542.6025186/94‑1 (MQ=60)
                   CTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGCGTTGTCGATTAC‑GGGGGTCG                                                                           <  SRR4302542.7064506/94‑1 (MQ=60)
                    TGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGCGTTGTCGATTAC‑GGGGGTCGA                                                                          <  SRR4302542.4877824/94‑1 (MQ=60)
                     GAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGCGTTGTCGATTAC‑GGGGGTCGAG                                                                         <  SRR4302542.4930466/94‑1 (MQ=60)
                     GAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGCGTTGTCGATTAC‑GGGGGTCGAG                                                                         <  SRR4302542.1358627/94‑1 (MQ=60)
                     GAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGCGTTGTCGATTAC‑GGGGGTCGAG                                                                         <  SRR4302542.4822474/94‑1 (MQ=60)
                     GAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGCGTTGTCGATTAC‑GGGGGTCGAG                                                                         >  SRR4302542.2481857/1‑94 (MQ=60)
                      AATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGCGTTGTCGATTAC‑GGGGGTCGAGG                                                                        <  SRR4302542.7435407/94‑1 (MQ=60)
                       ATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGCGTTGTCGATTAC‑GGGGGTCGAGGC                                                                       <  SRR4302542.1494934/94‑1 (MQ=60)
                        TCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGCGTTGTCGATTAC‑GGGGGGCGAGGCG                                                                      >  SRR4302542.566611/1‑94 (MQ=60)
                         CCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGCGTTGTCGATTAC‑GGGGGTCGAGGCGC                                                                     >  SRR4302542.6996276/1‑94 (MQ=60)
                          CAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTAC‑GGGGGTCGAGGTGCT                                                                    >  SRR4302542.4759998/1‑94 (MQ=60)
                          CAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGCGTTGTCGATTAC‑GGGGGTCGAGGCGCT                                                                    <  SRR4302542.5565401/94‑1 (MQ=60)
                          CAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGCGTTGTCGATTAC‑GGGGGTCGAGGCGCT                                                                    >  SRR4302542.2904858/1‑94 (MQ=60)
                           AATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTAC‑GGGGGTCGAGGTGCTG                                                                   >  SRR4302542.4934605/1‑94 (MQ=60)
                           AATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGCGTTGTCGATTAC‑GGGGGTCGAGGCGCTG                                                                   >  SRR4302542.576516/1‑94 (MQ=60)
                             TGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGCGTTGTCGATTAC‑GGGGGTCGAGGCGCTGTA                                                                 <  SRR4302542.7172243/94‑1 (MQ=60)
                             TGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGCGTTGTCGATTAC‑GGGGGTCGAGGCGCTGTA                                                                 <  SRR4302542.4520045/94‑1 (MQ=60)
                              GTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGCGTTGTCGATTAC‑GGGGGTCGAGGCGCTGTAT                                                                <  SRR4302542.2965294/94‑1 (MQ=60)
                              GTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGCGTTGTCGATTAC‑GGGGGTCGAGGCGCTGTAT                                                                >  SRR4302542.4045693/1‑94 (MQ=60)
                              GTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGCGTTGTCGATTAC‑GGGGGTCGAGGCGCTGTAT                                                                <  SRR4302542.7326348/94‑1 (MQ=60)
                              GTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGCGTTGTCGATTAC‑GGGGGTCGAGGCGCTGTAT                                                                <  SRR4302542.4445532/94‑1 (MQ=60)
                               TGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGCGTTGTCGATTAC‑GGGGGTCGAGGCGCTGTATG                                                               <  SRR4302542.1119018/94‑1 (MQ=60)
                               TGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGCGTTGTCGATTAC‑GGGGGTCGAGGCGCTGTATG                                                               >  SRR4302542.2990143/1‑94 (MQ=60)
                                GGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGCGTTGTCGATTAC‑GGGGGTCGAGGCGCTGTATGC                                                              >  SRR4302542.1265056/1‑94 (MQ=60)
                                GGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGCGTTGTCGATTAC‑GGGGGTCGAGGCGCTGTATGC                                                              >  SRR4302542.1139556/1‑94 (MQ=60)
                                GGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGCGTTGTCGATTAC‑GGGGGTCGAGGCGCTGTATGC                                                              <  SRR4302542.1810895/94‑1 (MQ=60)
                                 GGCGGTTCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGCGTTGTCGATTAC‑GGGGGTCGAGGCGCTGTATGCT                                                             <  SRR4302542.4223736/94‑1 (MQ=60)
                                 GGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGCGTTGTCGATTAC‑GGGGGTCGAGGCGCTGTATGCT                                                             >  SRR4302542.6472472/1‑94 (MQ=60)
                                 GGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGCGTTGTCGATTAC‑GGGGGTCGAGGCGCTGTATGCT                                                             <  SRR4302542.3673858/94‑1 (MQ=60)
                                   CGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTAC‑GGGGGTCGAGGTGCTGTATGCTGA                                                           >  SRR4302542.6890045/1‑94 (MQ=60)
                                   CGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGCGTTGTCGATTAC‑GGGGGTCGAGGCGCTGTATGCTGA                                                           >  SRR4302542.5836403/1‑94 (MQ=60)
                                   CGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGCGTTGTCGATTAC‑GGGGGTCGAGGCGCTGTATGCTGA                                                           <  SRR4302542.3005189/94‑1 (MQ=60)
                                      TGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGCGTTGTCGATTAC‑GGGGGTCGAGGCGCTGTATGCTGATAT                                                        >  SRR4302542.7031728/1‑94 (MQ=60)
                                      TGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGCGTTGTCGATTAC‑GGGGGTCGAGGCGCTGTATGCTGATAT                                                        <  SRR4302542.1084950/94‑1 (MQ=60)
                                      TGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGCGTTGTCGATTAC‑GGGGGTCGAGGCGCTGTATGCTGATAT                                                        >  SRR4302542.1014233/1‑94 (MQ=60)
                                        CATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTAC‑GGGGGTCGAGGTGCTGTATGCTGATATGG                                                      <  SRR4302542.1650959/94‑1 (MQ=60)
                                        CATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGCGTTGTCGATTAC‑GGGGGTCGAGGCGCTGTATGCTGATATGG                                                      <  SRR4302542.3862338/94‑1 (MQ=60)
                                        CATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGCGTTGTCGATTAC‑GGGGGTCGAGGCGCTGTATGCTGATATGG                                                      >  SRR4302542.4244551/1‑94 (MQ=60)
                                         ATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTGGGGGCAGTGGCGTTGTCGATTAC‑GGGGGTCGAGGCGCTGTATGCTGATATGGG                                                     >  SRR4302542.6282283/1‑94 (MQ=60)
                                         ATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGCGTTGTCGATTAC‑GGGGGTCGAGGCGCTGTATGCTGATATGGG                                                     >  SRR4302542.2702623/1‑94 (MQ=60)
                                         ATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGCGTTGTCGATTAC‑GGGGGTCGAGGCGCTGTATGCTGATATGGG                                                     >  SRR4302542.3759447/1‑94 (MQ=60)
                                         ATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGCGTTGTCGATTAC‑GGGGGTCGAGGCGCTGTATGCTGATATGGG                                                     >  SRR4302542.5066376/1‑94 (MQ=60)
                                           TTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTAC‑GGGGGTCGAGGTGCTGTATGCTGATATGGGGC                                                   >  SRR4302542.7372015/1‑94 (MQ=60)
                                           TTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGCGTTGTCGATTAC‑GGGGGTCGAGGCGCTGTATGCTGATATGGGGC                                                   <  SRR4302542.4147155/94‑1 (MQ=60)
                                           TTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGCGTTGTCGATTAC‑GGGGGTCGAGGCGCTGTATGCTGATATGGGGC                                                   >  SRR4302542.1525400/1‑94 (MQ=60)
                                            TCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGCGTTGTCGATTAC‑GGGGGTCGAGGCGCTGTATGCTGATATGGGGCA                                                  >  SRR4302542.5781576/1‑94 (MQ=60)
                                            TCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGCGTTGTCGATTAC‑GGGGGTCGAGGCGCTGTATGCTGATATGGGGCA                                                  >  SRR4302542.1488091/1‑94 (MQ=60)
                                            TCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGCGTTGTCGATTAC‑GGGGGTCGAGGCGCTGTATGCTGATATGGGGCA                                                  >  SRR4302542.4645357/1‑94 (MQ=60)
                                             CTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGCGTTGTCGATTAC‑GGGGGTCGAGGCGCTGTATGCTGATATGGGGCAC                                                 >  SRR4302542.5646695/1‑94 (MQ=60)
                                              TTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGCGTTGTCGATTAC‑GGGGGTCGAGGCGCTGTATGCTGATATGGGGCACT                                                >  SRR4302542.122707/1‑94 (MQ=60)
                                               TCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGCGTTGTCGATTAC‑GGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTT                                               >  SRR4302542.2435070/1‑94 (MQ=60)
                                                 CTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTAC‑GGGGGTCGAGGTGCTGTATGCTGATATGGGGCACTTTG                                             >  SRR4302542.2815784/1‑94 (MQ=60)
                                                 CTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGCGTTGTCGATTAC‑GGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTG                                             <  SRR4302542.4269338/94‑1 (MQ=60)
                                                   TGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGCGTTGTCGATTAC‑GGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGT                                           >  SRR4302542.624200/1‑94 (MQ=60)
                                                    GAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGCGTTGTCGATTAC‑GGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTA                                          <  SRR4302542.5979169/94‑1 (MQ=60)
                                                    GAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGCGTTGTCGATTAC‑GGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTA                                          >  SRR4302542.7439676/1‑94 (MQ=60)
                                                     AATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTAC‑GGGGGTCGAGGTGCTGTATGCTGATATGGCGCACTTTGGTAA                                         <  SRR4302542.2558930/94‑1 (MQ=60)
                                                     AATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGCGTTGTCGATTAC‑GGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAA                                         >  SRR4302542.436993/1‑94 (MQ=60)
                                                      ATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGCGTTGTCGATTAC‑GGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAG                                        >  SRR4302542.6102720/1‑94 (MQ=60)
                                                      ATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGCGTTGTCGATTAC‑GGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAG                                        <  SRR4302542.4892625/94‑1 (MQ=60)
                                                       TACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGCGTTGTCGATTAC‑GGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGT                                       >  SRR4302542.5133372/1‑94 (MQ=60)
                                                       TACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGCGTTGTCGATTAC‑GGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGT                                       <  SRR4302542.4286152/94‑1 (MQ=60)
                                                        ACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGCGTTGTCGATTAC‑GGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTT                                      >  SRR4302542.4886658/1‑94 (MQ=60)
                                                         CAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTAC‑GGGGGTCGAGGTGCTGTATGCTGATATGGGGCACTTTGGTAAGTTC                                     <  SRR4302542.146067/94‑1 (MQ=60)
                                                         CAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTAC‑GAGGGTCGAGGTGCTGTATGCTGATATGGGGCACTTTGGTAAGTTC                                     <  SRR4302542.5222917/94‑1 (MQ=60)
                                                         CAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGCGTTGTCGATTAC‑GGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTC                                     <  SRR4302542.4458985/94‑1 (MQ=60)
                                                         CAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGCGTTGTCGATTAC‑GGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTC                                     <  SRR4302542.6240761/94‑1 (MQ=60)
                                                          AAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGCGTTGTCGATTAC‑GGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCC                                    <  SRR4302542.2581369/94‑1 (MQ=60)
                                                          AAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGCGTTGTCGATTAC‑GGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCC                                    >  SRR4302542.6305898/1‑94 (MQ=60)
                                                           AAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTACGGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCC                                    <  SRR4302542.846871/94‑1 (MQ=60)
                                                            AACGGTTTCTTTTATTGCATTAGGGGCAGTGGCGTTGTCGATTAC‑GGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCT                                  <  SRR4302542.7276315/94‑1 (MQ=60)
                                                             ACGGTTTCTTTTATTGCATTAGGGGCAGTGGCGTTGTCGATTAC‑GGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTA                                 >  SRR4302542.6260473/1‑94 (MQ=60)
                                                              CGGTTTCTTTTATTGCATTAGGGGCAGTGGCGTTGTCGATTAC‑GGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTAT                                <  SRR4302542.4956703/94‑1 (MQ=60)
                                                              CGGTTTCTTTTATTGCATTAGGGGCAGTGGCGTTGTCGATTAC‑GGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTAT                                >  SRR4302542.2887587/1‑94 (MQ=60)
                                                              CGGTTTCTTTTATTGCATTAGGGGCAGTGGCGTTGTCGATTAC‑GGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTAT                                <  SRR4302542.1406960/94‑1 (MQ=60)
                                                              CGGTTTCTTTTATTGCATTAGGGGCAGTGGCGTTGTCGATTAC‑GGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTAT                                >  SRR4302542.5541530/1‑94 (MQ=60)
                                                               GGTTTCTTTTATTGCATTAGGGGCAGTGGCGTTGTCGATTAC‑GGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATT                               <  SRR4302542.777692/94‑1 (MQ=60)
                                                               GGTTTCTTTTATTGCATTAGGGGCAGTGGCGTTGTCGATTAC‑GGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATT                               <  SRR4302542.7277980/94‑1 (MQ=60)
                                                               GGTTTCTTTTATTGCATTAGGGGCAGTGGCGTTGTCGATTAC‑GGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATT                               >  SRR4302542.1086952/1‑94 (MQ=60)
                                                               GGTTTCTTTTATTGCATTAGGGGCAGTGGCGTTGTCGATTAC‑GGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATT                               >  SRR4302542.4486345/1‑94 (MQ=60)
                                                                 TTTCTTTTATTGCATTAGGGGCAGTGGCGTTGTCGATTAC‑GGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCG                             <  SRR4302542.6250962/94‑1 (MQ=60)
                                                                  TTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTAC‑GGGGGTCGAGGTGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGC                            >  SRR4302542.2782849/1‑94 (MQ=60)
                                                                  TTCTTTTATTGCATTAGGGGCAGTGGCGTTGTCGATTAC‑GGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGC                            >  SRR4302542.6657462/1‑94 (MQ=60)
                                                                  TTCTTTTATTGCATTAGGGGCAGTGGCGTTGTCGATTAC‑GGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGC                            >  SRR4302542.387826/1‑94 (MQ=60)
                                                                    CTTTTATTGCATTAGGGGCAGTGGCGTTGTCGATTAC‑GGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCT                          <  SRR4302542.5170082/94‑1 (MQ=60)
                                                                     TTTTATTGCATTAGGGGCAGTGGCGTTGTCGATTAC‑GGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTG                         >  SRR4302542.5715715/1‑94 (MQ=60)
                                                                      TTTATTGCATTAGGGGCAGTGGCGTTGTCGATTAC‑GGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGG                        <  SRR4302542.2299893/94‑1 (MQ=60)
                                                                      TTTATTGCATTAGGGGCAGTGGCGTTGTCGATTAC‑GGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGG                        <  SRR4302542.4073942/94‑1 (MQ=60)
                                                                      TTTATTGCATTAGGGGCAGTGGCGTTGTCGATTAC‑GGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGG                        <  SRR4302542.4073943/94‑1 (MQ=60)
                                                                          TTGCATTAGGGGCAGTGGCGTTGTCGATTAC‑GGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTG                    <  SRR4302542.1178400/94‑1 (MQ=60)
                                                                           TGCATTAGGGGCAGTGGTGTTGTCGATTAC‑GGGGGTCGAGGTGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGG                   <  SRR4302542.1942320/94‑1 (MQ=60)
                                                                           TGCATTAGGGGCAGTGGCGTTGTCGATTAC‑GGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGG                   <  SRR4302542.2498558/94‑1 (MQ=60)
                                                                           TGCATTAGGGGCAGTGGCGTTGTCGATTAC‑GGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGG                   >  SRR4302542.2211618/1‑94 (MQ=60)
                                                                            GCATTAGGGGCAGTGGCGTTGTCGATTAC‑GGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGT                  <  SRR4302542.3473514/94‑1 (MQ=60)
                                                                             CATTAGGGGCAGTGGCGTTGTCGATTAC‑GGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTT                 >  SRR4302542.2717062/1‑94 (MQ=60)
                                                                              ATTAGGGGCAGTGGCGTTGTCGATTAC‑GGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTC                >  SRR4302542.7590988/1‑94 (MQ=60)
                                                                              ATTAGGGGCAGTGGCGTTGTCGATTAC‑GGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTC                <  SRR4302542.3779286/94‑1 (MQ=60)
                                                                                TAGGGGCAGTGGTGTTGTCGATTAC‑GGGGGTCGAGGTGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCAC              >  SRR4302542.3804583/1‑94 (MQ=60)
                                                                                  GGGGCAGTGGCGTTGTCGATTAC‑GGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACCG            >  SRR4302542.7246839/1‑94 (MQ=60)
                                                                                  GGGGCAGTGGCGTTGTCGATTAC‑GGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACCG            >  SRR4302542.3222219/1‑94 (MQ=60)
                                                                                    GGCAGTGGCGTTGTCGATTAC‑GGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACCGTC          >  SRR4302542.3572037/1‑94 (MQ=60)
                                                                                    GGCAGTGGCGTTGTCGATTAC‑GGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACCGTC          <  SRR4302542.3093081/94‑1 (MQ=60)
                                                                                    GGCAGTGGCGTTGTCGATTAC‑GGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACCGTC          <  SRR4302542.1373789/94‑1 (MQ=60)
                                                                                       AGTGGCGTTGTCGATTAC‑GGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACCGTCGTA       >  SRR4302542.2807058/1‑94 (MQ=60)
                                                                                       AGTGGCGTTGTCGATTAC‑GGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACCGTCGTA       >  SRR4302542.4514952/1‑94 (MQ=60)
                                                                                       AGTGGCGTTGTCGATTAC‑GGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACCGTCGTA       >  SRR4302542.3336342/1‑94 (MQ=60)
                                                                                       AGTGGCGTTGTCGATTAC‑GGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACCGTCGTA       >  SRR4302542.6811726/1‑94 (MQ=60)
                                                                                        GTGGCGTTGTCGATTAC‑GGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACCGTCGTAT      <  SRR4302542.4663648/94‑1 (MQ=60)
                                                                                        GTGGCGTTGTCGATTAC‑GGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACCGTCGTAT      <  SRR4302542.4511443/94‑1 (MQ=60)
                                                                                        GTGGCGTTGTCGATTAC‑GGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACCGTCGTAT      <  SRR4302542.2022226/94‑1 (MQ=60)
                                                                                         TGGTGTTGTCGATTAC‑GGGGGTCGAGGTGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACCGTCGTATT     <  SRR4302542.4841994/94‑1 (MQ=60)
                                                                                         TGGCGTTGTCGATTAC‑GGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACCGTCGTATT     <  SRR4302542.468060/94‑1 (MQ=60)
                                                                                         TGGCGTTGTCGATTAC‑GGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACCGTCGTATT     <  SRR4302542.2788947/94‑1 (MQ=60)
                                                                                          GGCGTTGTCGATTAC‑GGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACCGTCGTATTA    <  SRR4302542.3220377/94‑1 (MQ=60)
                                                                                           GTGTTGTCGATTAC‑GGGGGTCGAGGTGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACCGTCGTATTAC   <  SRR4302542.5262900/94‑1 (MQ=60)
                                                                                            CGTTGTCGATTAC‑GGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACCGTCGTATTACC  <  SRR4302542.214328/94‑1 (MQ=60)
                                                                                            CGTTGTCGATTAC‑GGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACCGTCGTATTACC  <  SRR4302542.3137311/94‑1 (MQ=60)
                                                                                            |                                                                                              
CCCGGAAGTGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTAC‑GGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACCGTCGTATTACC  >  NC_000913/3929515‑3929700

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑