Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1061 294.1 15065038 98.6% 14854127 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,950,557 2 bp→GC coding (451‑452/930 nt) ilvE → branched‑chain amino‑acid aminotransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,950,5570CG100.0% 1670.7 / NA 489R151A (CGA→GCA) ilvEbranched‑chain amino‑acid aminotransferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (220/269);  total (220/269)
*NC_0009133,950,5580GC100.0% 1645.7 / NA 489R151A (CGA→GCA) ilvEbranched‑chain amino‑acid aminotransferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (220/269);  total (220/269)

Only 100 of 489 total aligned reads displayed.

ATCGCTGCTTTCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCACGACCAAACACCATCCCGACGGCGGC‑AAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCGCCGCCACGGTT  >  NC_000913/3950470‑3950644
                                                                                       ||                                                                                       
aTCGCTGCTTTCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCa                                                                                     >  2:1313786/1‑93 (MQ=255)
   gctgctTTCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAac                                                                                  <  2:208639/93‑1 (MQ=255)
    ctgctTTCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAaca                                                                                 >  2:4187822/1‑93 (MQ=255)
     tgctTTCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAacac                                                                                >  1:754109/1‑93 (MQ=255)
      gctTTCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACAcc                                                                               >  2:2201801/1‑93 (MQ=255)
      gctTTCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACAcc                                                                               >  1:4948138/1‑93 (MQ=255)
       ctTTCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCa                                                                              >  1:1210104/1‑93 (MQ=255)
       ctTTCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCa                                                                              <  2:6464224/93‑1 (MQ=255)
       ctTTCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCa                                                                              <  2:225758/93‑1 (MQ=255)
        tttCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCAt                                                                             >  1:1441000/1‑93 (MQ=255)
        tttCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCAt                                                                             >  1:1920883/1‑93 (MQ=255)
          tCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATcc                                                                           >  2:1632344/1‑93 (MQ=255)
          tCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATcc                                                                           <  2:5802841/93‑1 (MQ=255)
           cccGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATccc                                                                          <  1:940973/93‑1 (MQ=255)
            ccGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCg                                                                         >  2:7042843/1‑93 (MQ=255)
            ccGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCg                                                                         <  1:2499048/93‑1 (MQ=255)
            ccGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCg                                                                         <  2:6714171/93‑1 (MQ=255)
             cGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGa                                                                        <  1:4483743/93‑1 (MQ=255)
                 gggAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGAcggc                                                                    >  2:919905/1‑93 (MQ=255)
                 gggAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGAcggc                                                                    >  2:5421819/1‑93 (MQ=255)
                  ggAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGAcggcg                                                                   >  1:3374140/1‑93 (MQ=255)
                  ggAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGAcggcg                                                                   >  2:5267588/1‑93 (MQ=255)
                    aGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACggcggc                                                                 <  2:379028/93‑1 (MQ=255)
                        tATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGC‑aaaa                                                            <  2:5074874/93‑1 (MQ=255)
                         aTCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGC‑AAAAg                                                           <  2:4880394/93‑1 (MQ=255)
                         aTCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGC‑AAAAg                                                           <  2:6602217/93‑1 (MQ=255)
                          tCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGC‑AAAAGc                                                          <  2:4001188/93‑1 (MQ=255)
                           cTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGC‑AAAAGcc                                                         <  1:3757044/93‑1 (MQ=255)
                              ggCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGC‑AAAAGCCggt                                                      <  2:3154980/93‑1 (MQ=255)
                              ggCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGC‑AAAAGCCggt                                                      <  1:7173931/93‑1 (MQ=255)
                              ggCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGCTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGC‑AAAAGCCggt                                                      <  2:2660732/93‑1 (MQ=255)
                               gcgcAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGC‑AAAAGCCggtg                                                     <  1:2725419/93‑1 (MQ=255)
                               gcgcAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGC‑AAAAGCCggtg                                                     <  2:5547191/93‑1 (MQ=255)
                                   aGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGC‑AAAAGCCGGTGGTaa                                                 <  1:5523015/93‑1 (MQ=255)
                                   aGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGC‑AAAAGCCGGTGGTaa                                                 <  1:3515078/93‑1 (MQ=255)
                                   aGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGC‑AAAAGCCGGTGGTaa                                                 <  2:6064936/93‑1 (MQ=255)
                                   aGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGC‑AAAAGCCGGTGGTaa                                                 <  1:668582/93‑1 (MQ=255)
                                   aGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCCCCTGGAACCGCGCAGCACAAAACCCCATCCCGAGGGCGGC‑AAAAGCCGGTGGTaa                                                 <  1:3656103/93‑1 (MQ=255)
                                    gAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGC‑AAAAGCCGGTGGTAAc                                                >  2:5996906/1‑93 (MQ=255)
                                    gAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGC‑AAAAGCCGGTGGTAAc                                                >  1:4310569/1‑93 (MQ=255)
                                     aaGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGC‑AAAAGCCGGTGGTAACt                                               >  2:4758381/1‑93 (MQ=255)
                                      aGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGC‑AAAAGCCGGTGGTAACTa                                              >  1:2599166/1‑93 (MQ=255)
                                        cgcTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGC‑AAAAGCCGGTGGTAACTAcc                                            <  2:6302125/93‑1 (MQ=255)
                                         gcTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGC‑AAAAGCCGGTGGTAACTACct                                           <  1:5297329/93‑1 (MQ=255)
                                         gcTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGC‑AAAAGCCGGTGGTAACTACct                                           <  1:4553014/93‑1 (MQ=255)
                                         gcTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGC‑AAAAGCCGGTGGTAACTACct                                           >  2:531010/1‑93 (MQ=255)
                                          cTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGC‑AAAAGCCGGTGGTAACTACctc                                          <  2:1174750/93‑1 (MQ=255)
                                             gAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGC‑AAAAGCCGGTGGTAACTACctctct                                       <  2:5674847/93‑1 (MQ=255)
                                             gAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGC‑AAAAGCCGGTGGTAACTACctctct                                       <  2:5014273/93‑1 (MQ=255)
                                               gCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGC‑AAAAGCCGGTGGTAACTACCTCTCTTc                                     <  2:4278581/93‑1 (MQ=255)
                                                 aGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGC‑AAAAGCCGGTGGTAACTACCTCTCTTccc                                   <  2:6179742/93‑1 (MQ=255)
                                                   gggATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGC‑AAAAGCCGGTGGTAACTACCTCTCTTCCctg                                 >  1:2247874/1‑93 (MQ=255)
                                                    ggATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGC‑AAAAGCCGGTGGTAACTACCTCTCTTCCctgc                                <  2:4449549/93‑1 (MQ=255)
                                                    ggATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGC‑AAAAGCCGGTGGTAACTACCTCTCTTCCctgc                                >  1:169937/1‑93 (MQ=255)
                                                     gATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGC‑AAAAGCCGGTGGTAACTACCTCTCTTCCctgct                               >  2:274739/1‑93 (MQ=255)
                                                       tCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAGCACCATCCCGACGGCGGCAAAAAGCGGGGGGCAATTACTTTCCTTCCctccgg                              >  1:6217872/1‑89 (MQ=255)
                                                       tCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGC‑AAAAGCCGGTGGTAACTACCTCTCTTCCCTGCtgg                             >  1:5087831/1‑93 (MQ=255)
                                                        cgatgcgatgGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGC‑AAAAGCCGGTGGTAACTACCTCTCTTCCCTGCtggt                            <  1:3928533/93‑1 (MQ=255)
                                                         gatgcgatgGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGC‑AAAAGCCGGTGGTAACTACCTCTCTTCCCTGCtggtg                           >  2:2866057/1‑93 (MQ=255)
                                                         gatgcgatgGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGC‑AAAAGCCGGTGGTAACTACCTCTCTTCCCTGCtggtg                           >  2:4356787/1‑93 (MQ=255)
                                                         gatgcgatgGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGC‑AAAAGCCGGTGGTAACTACCTCTCTTCCCTGCtggtg                           >  2:3624308/1‑93 (MQ=255)
                                                         gatgcgatgGTTTCCTCCTGGAACCGCACAGCACCAAACACCATCCCGACGGCGGC‑AAAAGCCGGTGGTAACTACCTCTCTTCCCTGCtggtg                           <  1:1068050/93‑1 (MQ=255)
                                                          atgcgatgGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGC‑AAAAGCCGGTGGTAACTACCTCTCTTCCCTGCtggtgg                          >  1:4243058/1‑93 (MQ=255)
                                                           tgcgatgGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGC‑AAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTggg                         <  1:6110147/93‑1 (MQ=255)
                                                           tgcgatgGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGC‑AAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTggg                         <  1:3446457/93‑1 (MQ=255)
                                                           tgcgatgGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCAGACGGCGGC‑AAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTggg                         >  1:5984098/1‑93 (MQ=255)
                                                            gcgatgGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGC‑AAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGt                        <  1:2026556/93‑1 (MQ=255)
                                                            gcgatgGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGC‑AAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGt                        >  2:6963070/1‑93 (MQ=255)
                                                             cgatgGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGC‑AAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTa                       <  1:3017426/93‑1 (MQ=255)
                                                             cgatgGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGC‑AAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTa                       >  2:3947614/1‑93 (MQ=255)
                                                             cgatgGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGC‑AAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTa                       <  1:5741682/93‑1 (MQ=255)
                                                              gatgGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGC‑AAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAg                      >  1:6579708/1‑93 (MQ=255)
                                                               atgGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGC‑AAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGc                     >  2:773809/1‑93 (MQ=255)
                                                                tgGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGC‑AAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCg                    >  2:7374914/1‑93 (MQ=255)
                                                                tgGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGC‑AAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCg                    >  1:7318484/1‑93 (MQ=255)
                                                                 ggTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGC‑AAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGa                   <  1:6859387/93‑1 (MQ=255)
                                                                 ggTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGC‑AAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGa                   >  1:1566096/1‑93 (MQ=255)
                                                                  gTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGC‑AAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGaa                  <  2:4604996/93‑1 (MQ=255)
                                                                  gTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGC‑AAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGaa                  <  2:1918603/93‑1 (MQ=255)
                                                                    ttCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGC‑AAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAgc                >  1:1296908/1‑93 (MQ=255)
                                                                    ttCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGC‑AAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAgc                <  1:4082143/93‑1 (MQ=255)
                                                                     tcctccTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGC‑AAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAgcg               <  2:3393223/93‑1 (MQ=255)
                                                                     tcctccTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGC‑AAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAgcg               >  1:890602/1‑93 (MQ=255)
                                                                       ctcctGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGC‑AAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAgcgcg             >  1:2364721/1‑93 (MQ=255)
                                                                        tcctGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGC‑AAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAgcgcgc            <  2:3462202/93‑1 (MQ=255)
                                                                          ctGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGC‑AAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGcgccg          >  2:3209406/1‑93 (MQ=255)
                                                                           tGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGC‑AAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGcgccgc         >  1:2876254/1‑93 (MQ=255)
                                                                           tGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGC‑AAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGcgccgc         >  1:3146226/1‑93 (MQ=255)
                                                                           tGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGC‑AAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGcgccgc         <  2:7461878/93‑1 (MQ=255)
                                                                           tGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGC‑AAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGcgccgc         >  1:5866799/1‑93 (MQ=255)
                                                                           tGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGC‑AAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGcgccgc         >  2:3533072/1‑93 (MQ=255)
                                                                            ggAACCGCGCAGCACCAAACACCATCCCGACGGCGGC‑AAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCgccgcc        >  2:1753384/1‑93 (MQ=255)
                                                                             gacccGCGCAGCACCAAACCCCATCCCGACGGCGGC‑AAAAGCCGGTGGTAACTCCCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCGCCGCCa       <  1:5672394/90‑1 (MQ=255)
                                                                             gAACCGCGCAGCACCAAACACCATCCCGACGGCGGC‑AAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCGCCGCCa       <  1:4890845/93‑1 (MQ=255)
                                                                             gAACCGCGCAGCACCAAACACCATCCCGACGGCGGC‑AAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCGCCGCCa       <  2:7214182/93‑1 (MQ=255)
                                                                             gAACCGCGCAGCACCAAACACCATCCCGACGGCGGC‑AAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCGCCGCCa       <  2:511572/93‑1 (MQ=255)
                                                                             gAACCGCGCAGCACCAAACACCATCCCGACGGCGGC‑AAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCGCCGCCa       <  2:249318/93‑1 (MQ=255)
                                                                             gAACCGCGCAGCACCAAACACCATCCCGACGGCGGC‑AAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCGCCGCCa       >  2:3087186/1‑93 (MQ=255)
                                                                              aaCCGCGCAGCACCAAACACCATCCCGACGGCGGC‑AAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCGCCGCCAc      <  2:1452700/93‑1 (MQ=255)
                                                                                ccGCGCAGCACCAAACACCATCCCGACGGCGGC‑AAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCGCCGCCACgg    >  1:5139098/1‑93 (MQ=255)
                                                                                       ||                                                                                       
ATCGCTGCTTTCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCACGACCAAACACCATCCCGACGGCGGC‑AAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCGCCGCCACGGTT  >  NC_000913/3950470‑3950644

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 240 total aligned reads displayed.
GTGATTATCGCTGCTTTCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCACGACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCGCCGCCACGGTTAT  >  NC_000913/3950464‑3950646
                                                                                             |                                                                                         
GTGATTATCGCTGCTTTCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAG                                                                                           <  SRR4302542.762426/94‑1 (MQ=60)
GTGATTATCGCTGCTTTCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAG                                                                                           >  SRR4302542.4342512/1‑94 (MQ=60)
GTGATTATCGCTGCTTTCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAG                                                                                           >  SRR4302542.4631537/1‑94 (MQ=60)
     TATCGCTGCTTTCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCA                                                                                      >  SRR4302542.2476114/1‑94 (MQ=60)
     TATCGCTGCTTTCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCA                                                                                      >  SRR4302542.7021335/1‑94 (MQ=60)
      ATCGCTGCTTTCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAA                                                                                     >  SRR4302542.5192367/1‑94 (MQ=60)
        CGCTGCTTTCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAAC                                                                                   <  SRR4302542.3121718/94‑1 (MQ=60)
        CGCTGCTTTCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAAC                                                                                   >  SRR4302542.5879079/1‑94 (MQ=60)
        CGCTGCTTTCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGACCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAAC                                                                                   >  SRR4302542.6803156/1‑94 (MQ=60)
         GCTGCTTTCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACA                                                                                  <  SRR4302542.3025154/94‑1 (MQ=60)
          CTGCTTTCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACAC                                                                                 >  SRR4302542.911760/1‑94 (MQ=60)
          CTGCTTTCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACAC                                                                                 >  SRR4302542.1494379/1‑94 (MQ=60)
           TGCTTTCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACC                                                                                >  SRR4302542.4996213/1‑94 (MQ=60)
            GCTTTCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCA                                                                               <  SRR4302542.7346618/94‑1 (MQ=60)
             CTTTCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCAT                                                                              >  SRR4302542.1453445/1‑94 (MQ=60)
             CTTTCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCAT                                                                              >  SRR4302542.4323726/1‑94 (MQ=60)
             CTTTCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCAT                                                                              >  SRR4302542.4143700/1‑94 (MQ=60)
             CTTTCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCAT                                                                              <  SRR4302542.3149306/94‑1 (MQ=60)
             CTTTCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCAT                                                                              >  SRR4302542.906947/1‑94 (MQ=60)
             CTTTCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCAT                                                                              >  SRR4302542.1937473/1‑94 (MQ=60)
             CTTTCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGAAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCAT                                                                              >  SRR4302542.4980586/1‑94 (MQ=60)
              TTTCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATC                                                                             >  SRR4302542.2755511/1‑94 (MQ=60)
              TTTCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATC                                                                             <  SRR4302542.959906/94‑1 (MQ=60)
                TCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCC                                                                           <  SRR4302542.1135337/94‑1 (MQ=60)
                TCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCC                                                                           >  SRR4302542.4530137/1‑94 (MQ=60)
                 CCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCG                                                                          <  SRR4302542.4554109/94‑1 (MQ=60)
                 CCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCG                                                                          <  SRR4302542.949069/94‑1 (MQ=60)
                 CCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCG                                                                          >  SRR4302542.3623633/1‑94 (MQ=60)
                 CCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCG                                                                          <  SRR4302542.3410141/94‑1 (MQ=60)
                  CCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGA                                                                         <  SRR4302542.2523281/94‑1 (MQ=60)
                   CGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGAC                                                                        <  SRR4302542.4527751/94‑1 (MQ=60)
                   CGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGAC                                                                        <  SRR4302542.7459200/94‑1 (MQ=60)
                     TGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACAGCGCAGCACCAAACACCATCCCGACGG                                                                      >  SRR4302542.4865517/1‑94 (MQ=60)
                      GGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGC                                                                     >  SRR4302542.3447726/1‑94 (MQ=60)
                      GGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGACCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCTTCAAACACCATCCCGACGGC                                                                     <  SRR4302542.3772095/94‑1 (MQ=60)
                       GGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCG                                                                    >  SRR4302542.3405838/1‑94 (MQ=60)
                        GGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGG                                                                   <  SRR4302542.3766236/94‑1 (MQ=60)
                        GGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGG                                                                   <  SRR4302542.2634859/94‑1 (MQ=60)
                         GAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGC                                                                  >  SRR4302542.1755702/1‑94 (MQ=60)
                            CGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAA                                                               >  SRR4302542.6515267/1‑94 (MQ=60)
                            CGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAA                                                               >  SRR4302542.3105395/1‑94 (MQ=60)
                             GTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAA                                                              <  SRR4302542.2757593/94‑1 (MQ=60)
                              TATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAG                                                             <  SRR4302542.1758939/94‑1 (MQ=60)
                              TATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAG                                                             <  SRR4302542.1822533/94‑1 (MQ=60)
                              TATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAG                                                             <  SRR4302542.6004845/94‑1 (MQ=60)
                                TCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCC                                                           <  SRR4302542.6291295/94‑1 (MQ=60)
                                TCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCC                                                           <  SRR4302542.2370730/94‑1 (MQ=60)
                                 CTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCG                                                          >  SRR4302542.535037/1‑94 (MQ=60)
                                 CTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCG                                                          >  SRR4302542.7473807/1‑94 (MQ=60)
                                 CTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCG                                                          <  SRR4302542.3794214/94‑1 (MQ=60)
                                  TGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGG                                                         <  SRR4302542.1658868/94‑1 (MQ=60)
                                  TGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGG                                                         >  SRR4302542.931461/1‑94 (MQ=60)
                                  TGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGG                                                         <  SRR4302542.1644643/94‑1 (MQ=60)
                                   GGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGT                                                        <  SRR4302542.2646252/94‑1 (MQ=60)
                                   GGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGT                                                        >  SRR4302542.6892865/1‑94 (MQ=60)
                                    GGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTG                                                       <  SRR4302542.7179386/94‑1 (MQ=60)
                                    GGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTG                                                       <  SRR4302542.7244303/94‑1 (MQ=60)
                                    GGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTG                                                       >  SRR4302542.4183858/1‑94 (MQ=60)
                                     GCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGG                                                      <  SRR4302542.3296535/94‑1 (MQ=60)
                                     GCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGG                                                      <  SRR4302542.6193957/94‑1 (MQ=60)
                                     GCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGG                                                      <  SRR4302542.5304538/94‑1 (MQ=60)
                                     GCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGG                                                      <  SRR4302542.2751714/94‑1 (MQ=60)
                                     GCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGG                                                      <  SRR4302542.675462/94‑1 (MQ=60)
                                      CGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGT                                                     >  SRR4302542.4542795/1‑94 (MQ=60)
                                      CGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCATCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGT                                                     <  SRR4302542.7252557/94‑1 (MQ=60)
                                       GCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTA                                                    <  SRR4302542.3826501/94‑1 (MQ=60)
                                       GCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTA                                                    <  SRR4302542.286632/94‑1 (MQ=60)
                                         AGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAAC                                                  >  SRR4302542.3251716/1‑94 (MQ=60)
                                         AGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAAC                                                  >  SRR4302542.4352932/1‑94 (MQ=60)
                                         AGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAAC                                                  >  SRR4302542.2154794/1‑94 (MQ=60)
                                         AGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAAC                                                  >  SRR4302542.4045089/1‑94 (MQ=60)
                                         AGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAAC                                                  <  SRR4302542.257732/94‑1 (MQ=60)
                                         AGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAAC                                                  >  SRR4302542.3618966/1‑94 (MQ=60)
                                         AGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAAC                                                  <  SRR4302542.3547939/94‑1 (MQ=60)
                                         AGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAAC                                                  <  SRR4302542.5576693/94‑1 (MQ=60)
                                         AGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAAC                                                  <  SRR4302542.674355/94‑1 (MQ=60)
                                           AAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTA                                                >  SRR4302542.2624320/1‑94 (MQ=60)
                                           AAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTA                                                >  SRR4302542.4300080/1‑94 (MQ=60)
                                            AGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTAC                                               >  SRR4302542.2650739/1‑94 (MQ=60)
                                            AGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTAC                                               <  SRR4302542.5453816/94‑1 (MQ=60)
                                            AGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTAC                                               >  SRR4302542.373645/1‑94 (MQ=60)
                                            AGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTAC                                               <  SRR4302542.557493/94‑1 (MQ=60)
                                              CGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCT                                             <  SRR4302542.2492716/94‑1 (MQ=60)
                                              CGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCT                                             <  SRR4302542.6159324/94‑1 (MQ=60)
                                              CGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCT                                             <  SRR4302542.2372857/94‑1 (MQ=60)
                                               GCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTC                                            <  SRR4302542.5348805/94‑1 (MQ=60)
                                                CTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCT                                           <  SRR4302542.763701/94‑1 (MQ=60)
                                                CTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCT                                           <  SRR4302542.1267600/94‑1 (MQ=60)
                                                 TGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTC                                          >  SRR4302542.7588822/1‑94 (MQ=60)
                                                  GGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCT                                         <  SRR4302542.2976503/94‑1 (MQ=60)
                                                  GGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCT                                         <  SRR4302542.5798533/94‑1 (MQ=60)
                                                  GGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCT                                         <  SRR4302542.1258213/94‑1 (MQ=60)
                                                  GGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCT                                         <  SRR4302542.1457430/94‑1 (MQ=60)
                                                   GAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTT                                        <  SRR4302542.4575025/94‑1 (MQ=60)
                                                    AGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTC                                       >  SRR4302542.432157/1‑94 (MQ=60)
                                                     GCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCC                                      <  SRR4302542.5169132/94‑1 (MQ=60)
                                                     GCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCC                                      <  SRR4302542.1986681/94‑1 (MQ=60)
                                                      CAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCC                                     >  SRR4302542.7476224/1‑94 (MQ=60)
                                                      CAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCC                                     >  SRR4302542.2558806/1‑94 (MQ=60)
                                                      CAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCC                                     <  SRR4302542.4141060/94‑1 (MQ=60)
                                                       AGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCT                                    <  SRR4302542.5849116/94‑1 (MQ=60)
                                                       AGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCT                                    <  SRR4302542.3498027/94‑1 (MQ=60)
                                                       AGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCT                                    >  SRR4302542.6133194/1‑94 (MQ=60)
                                                       AGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCT                                    <  SRR4302542.2399440/94‑1 (MQ=60)
                                                       AGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCT                                    <  SRR4302542.2601234/94‑1 (MQ=60)
                                                        GGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTG                                   <  SRR4302542.5759775/94‑1 (MQ=60)
                                                        GGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTG                                   >  SRR4302542.2267205/1‑94 (MQ=60)
                                                         GGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGC                                  <  SRR4302542.6011393/94‑1 (MQ=60)
                                                         GGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGC                                  >  SRR4302542.3538217/1‑94 (MQ=60)
                                                         GGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGC                                  >  SRR4302542.171376/1‑94 (MQ=60)
                                                          GGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCT                                 <  SRR4302542.733751/94‑1 (MQ=60)
                                                          GGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCT                                 <  SRR4302542.1211739/94‑1 (MQ=60)
                                                          GGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCT                                 <  SRR4302542.6265269/94‑1 (MQ=60)
                                                           GATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTG                                >  SRR4302542.5892685/1‑94 (MQ=60)
                                                           GATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTG                                >  SRR4302542.2981327/1‑94 (MQ=60)
                                                           GATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTG                                <  SRR4302542.5056798/94‑1 (MQ=60)
                                                            ATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAGCACCATCCCGACGGCGGCAAAAagcggggggcaattactttccttccctccgg                               >  SRR4302542.6279115/1‑63 (MQ=60)
                                                            ATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGG                               >  SRR4302542.7329427/1‑94 (MQ=60)
                                                            ATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGG                               >  SRR4302542.797462/1‑94 (MQ=60)
                                                            ATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGG                               >  SRR4302542.5137285/1‑94 (MQ=60)
                                                              CGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTG                             <  SRR4302542.5733166/94‑1 (MQ=60)
                                                              CGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTG                             >  SRR4302542.5576759/1‑94 (MQ=60)
                                                              CGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTG                             >  SRR4302542.5758373/1‑94 (MQ=60)
                                                              CGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTG                             >  SRR4302542.1442209/1‑94 (MQ=60)
                                                              CGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTG                             >  SRR4302542.5155433/1‑94 (MQ=60)
                                                              CGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTG                             >  SRR4302542.2249864/1‑94 (MQ=60)
                                                              CGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTG                             <  SRR4302542.3967296/94‑1 (MQ=60)
                                                              CGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTG                             <  SRR4302542.344396/94‑1 (MQ=60)
                                                               GATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGG                            >  SRR4302542.4284876/1‑94 (MQ=60)
                                                               GATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGG                            >  SRR4302542.294462/1‑94 (MQ=60)
                                                               GATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGG                            <  SRR4302542.4755788/94‑1 (MQ=60)
                                                               GATGCGATGGTTTCCTCCTGGAACCGCACAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGG                            <  SRR4302542.1077233/94‑1 (MQ=60)
                                                                ATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGG                           >  SRR4302542.1229727/1‑94 (MQ=60)
                                                                ATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGG                           >  SRR4302542.3846591/1‑94 (MQ=60)
                                                                ATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGG                           <  SRR4302542.6658937/94‑1 (MQ=60)
                                                                ATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGG                           >  SRR4302542.4395313/1‑94 (MQ=60)
                                                                ATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCAGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGG                           >  SRR4302542.6042067/1‑94 (MQ=60)
                                                                 TGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGT                          <  SRR4302542.6169279/94‑1 (MQ=60)
                                                                 TGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGT                          <  SRR4302542.5047529/94‑1 (MQ=60)
                                                                 TGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGT                          >  SRR4302542.5457693/1‑94 (MQ=60)
                                                                 TGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGT                          <  SRR4302542.3478710/94‑1 (MQ=60)
                                                                  GCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTA                         <  SRR4302542.853125/94‑1 (MQ=60)
                                                                  GCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTA                         <  SRR4302542.3229343/94‑1 (MQ=60)
                                                                   CGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCACAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAG                        <  SRR4302542.6272222/94‑1 (MQ=60)
                                                                   CGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAG                        <  SRR4302542.3046253/94‑1 (MQ=60)
                                                                   CGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAG                        <  SRR4302542.5624255/94‑1 (MQ=60)
                                                                   CGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAG                        >  SRR4302542.144538/1‑94 (MQ=60)
                                                                   CGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAG                        >  SRR4302542.6644541/1‑94 (MQ=60)
                                                                   CGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAG                        <  SRR4302542.1122143/94‑1 (MQ=60)
                                                                   CGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAG                        >  SRR4302542.4112592/1‑94 (MQ=60)
                                                                    GATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGC                       >  SRR4302542.135165/1‑94 (MQ=60)
                                                                    GATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGC                       >  SRR4302542.312162/1‑94 (MQ=60)
                                                                     ATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGATGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCG                      >  SRR4302542.2150995/1‑94 (MQ=60)
                                                                     ATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCG                      >  SRR4302542.7390162/1‑94 (MQ=60)
                                                                     ATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCG                      >  SRR4302542.196706/1‑94 (MQ=60)
                                                                      TGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGA                     >  SRR4302542.1579621/1‑94 (MQ=60)
                                                                      TGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGA                     >  SRR4302542.5947084/1‑94 (MQ=60)
                                                                       GGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAA                    <  SRR4302542.6926787/94‑1 (MQ=60)
                                                                       GGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAA                    >  SRR4302542.409788/1‑94 (MQ=60)
                                                                        GTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAG                   <  SRR4302542.5728241/94‑1 (MQ=60)
                                                                         TTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGC                  >  SRR4302542.1308075/1‑94 (MQ=60)
                                                                          TTCCTCCTGGAAGCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCG                 <  SRR4302542.6718504/94‑1 (MQ=60)
                                                                          TTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCG                 >  SRR4302542.3527590/1‑94 (MQ=60)
                                                                          TTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCG                 <  SRR4302542.4122375/94‑1 (MQ=60)
                                                                          TTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCG                 <  SRR4302542.6941720/94‑1 (MQ=60)
                                                                          TTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCG                 <  SRR4302542.4623267/94‑1 (MQ=60)
                                                                           TCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAGGCGC                <  SRR4302542.1292182/94‑1 (MQ=60)
                                                                           TCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGC                <  SRR4302542.4991197/94‑1 (MQ=60)
                                                                            CCTCCTGGAACCGCGCAGCACCAAACGCCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCG               <  SRR4302542.3758304/94‑1 (MQ=60)
                                                                            CCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCG               >  SRR4302542.2385161/1‑94 (MQ=60)
                                                                            CCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCG               >  SRR4302542.288727/1‑94 (MQ=60)
                                                                             CTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCGC              >  SRR4302542.5171128/1‑94 (MQ=60)
                                                                              TCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCGCC             <  SRR4302542.6318387/94‑1 (MQ=60)
                                                                              TCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCGCC             <  SRR4302542.4947464/94‑1 (MQ=60)
                                                                              TCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCCGGTGGGTAGCGAAGCGCGCC             >  SRR4302542.4403340/1‑94 (MQ=60)
                                                                                CTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTGGCGAAGGGCGCCGC           >  SRR4302542.6651505/1‑94 (MQ=60)
                                                                                CTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCGCCGC           >  SRR4302542.1968462/1‑94 (MQ=60)
                                                                                CTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCGCCGC           <  SRR4302542.4932342/94‑1 (MQ=60)
                                                                                CTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCGCCGC           >  SRR4302542.5923676/1‑94 (MQ=60)
                                                                                CTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCGCCGC           >  SRR4302542.3176111/1‑94 (MQ=60)
                                                                                 TGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCGCCGCC          <  SRR4302542.5102860/94‑1 (MQ=60)
                                                                                 TGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCGCCGCC          >  SRR4302542.440110/1‑94 (MQ=60)
                                                                                  GGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCGCCGCCA         >  SRR4302542.5270006/1‑94 (MQ=60)
                                                                                   GACCCGCGCAGCACCAAACCCCATCCCGACGGCGGCAAAAGCCGGTGGTAACTCCCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCGCCGCCAC        <  SRR4302542.5727487/94‑1 (MQ=60)
                                                                                    AACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCGCCGCCACG       >  SRR4302542.1959515/1‑94 (MQ=60)
                                                                                    AACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCGCCGCCACG       >  SRR4302542.3660396/1‑94 (MQ=60)
                                                                                    AACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCGCCGCCACG       <  SRR4302542.1636109/94‑1 (MQ=60)
                                                                                    AACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCGCCGCCACG       <  SRR4302542.789423/94‑1 (MQ=60)
                                                                                     ACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCGCCGCCACGG      >  SRR4302542.5189049/1‑94 (MQ=60)
                                                                                       CGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCGCCGCCACGGTT    >  SRR4302542.6086204/1‑94 (MQ=60)
                                                                                       CGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCGCCGCCACGGTT    <  SRR4302542.2225356/94‑1 (MQ=60)
                                                                                       CGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGCGGGTAGCGAAGCGCGCCGCCACGGTT    >  SRR4302542.237495/1‑94 (MQ=60)
                                                                                        GCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCGCCGCCACGGTTA   <  SRR4302542.3834463/94‑1 (MQ=60)
                                                                                        GCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCGCCGCCACGGTTA   <  SRR4302542.1509175/94‑1 (MQ=60)
                                                                                         CGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCGCCGCCACGGTTAT  >  SRR4302542.115058/1‑94 (MQ=60)
                                                                                         CGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCGCCGCCACGGTTAT  <  SRR4302542.555344/94‑1 (MQ=60)
                                                                                         CGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCGCCGCCACGGTTAT  <  SRR4302542.494689/94‑1 (MQ=60)
                                                                                         CGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCGCCGCCACGGTTAT  <  SRR4302542.6159561/94‑1 (MQ=60)
                                                                                         CGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCGCCGCCACGGTTAT  >  SRR4302542.6523351/1‑94 (MQ=60)
                                                                                         CGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCGCCGCCACGGTTAT  >  SRR4302542.6768722/1‑94 (MQ=60)
                                                                                             |                                                                                         
GTGATTATCGCTGCTTTCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCACGACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCGCCGCCACGGTTAT  >  NC_000913/3950464‑3950646

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑