Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1061 294.1 15065038 98.6% 14854127 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,987,586 2 bp→GC coding (814‑815/963 nt) hemC ← porphobilinogen deaminase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,987,5860CG99.8% 1701.7 / NA 509R272A (CGG→GCG) hemCporphobilinogen deaminase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base G (295/213);  minor base T (0/1);  total (295/214)
Fisher's exact test for biased strand distribution p-value = 4.20e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*NC_0009133,987,5870GC100.0% 1693.1 / NA 509R272A (CGG→GCG) hemCporphobilinogen deaminase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (295/214);  total (295/214)

Only 100 of 513 total aligned reads displayed.

AGCTCTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCCGGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGACATCCGCCTTCGAGACGGG  >  NC_000913/3987499‑3987673
                                                                                       ||                                                                                      
aGCTCTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGgcgcgccg                                                                                    <  1:120593/93‑1 (MQ=255)
 gCTCTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGTGAACCGTCCGGCGCGCCGa                                                                                   >  1:1040803/1‑93 (MQ=255)
 gCTCTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGa                                                                                   >  1:2440502/1‑93 (MQ=255)
   tcttctGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGAcc                                                                                 >  1:84121/1‑93 (MQ=255)
   tcttctGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGAcc                                                                                 >  2:1566387/1‑93 (MQ=255)
    cttctGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCa                                                                                <  2:44492/93‑1 (MQ=255)
     ttctGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACCGCGGCGTTCACTGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAg                                                                               >  2:3804206/1‑93 (MQ=255)
      tctGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAgc                                                                              >  1:7386735/1‑93 (MQ=255)
        tGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAgcgc                                                                            >  1:5389004/1‑93 (MQ=255)
        tGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAgcgc                                                                            >  1:909069/1‑93 (MQ=255)
           cAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGcgcacg                                                                         >  2:6830475/1‑93 (MQ=255)
           cAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGcgcacg                                                                         >  2:1475599/1‑93 (MQ=255)
            aGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGcgcacgc                                                                        >  1:1774546/1‑93 (MQ=255)
            aGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGcgcacgc                                                                        >  1:3098021/1‑93 (MQ=255)
             gCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGcgcacgca                                                                       <  2:6338262/93‑1 (MQ=255)
             gCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGcgcacgca                                                                       >  2:4902607/1‑93 (MQ=255)
             gCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGcgcacgca                                                                       <  2:208948/93‑1 (MQ=255)
               gAAATCCCCATTTGTTCGGCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGc                                                                     >  2:792100/1‑93 (MQ=255)
                aaaTCCCCATTTGTTCGGCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGcc                                                                    <  2:2226927/93‑1 (MQ=255)
                 aaTCCCCATTTGTTCGGCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCa                                                                   <  1:5745162/93‑1 (MQ=255)
                  aTCCCCATTTGTTCGGCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAg                                                                  >  1:1556388/1‑93 (MQ=255)
                  aTCCCCATTTGTTCGGCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAg                                                                  >  1:4850913/1‑93 (MQ=255)
                   tCCCCATTTGTTCGGCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGa                                                                 >  2:6744169/1‑93 (MQ=255)
                   tCCCCATTTGTTCGGCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGa                                                                 >  2:217441/1‑93 (MQ=255)
                     cccATTTGTTCGGCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGAtt                                                               <  2:5177700/93‑1 (MQ=255)
                      ccATTTGTTCGGCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGAttt                                                              >  1:7283798/1‑93 (MQ=255)
                      ccATTTGTTCGGCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGAttt                                                              >  2:3216025/1‑93 (MQ=255)
                      ccATTTGTTCGGCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGAttt                                                              <  1:6618719/93‑1 (MQ=255)
                       gaTTTGTTCGGCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTc                                                             >  2:7407710/2‑93 (MQ=255)
                       cATTTGTTCGGCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTc                                                             <  2:7208234/93‑1 (MQ=255)
                       cATTTGTTCGGCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTc                                                             >  2:5693790/1‑93 (MQ=255)
                         tttGTTCGGCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGc                                                           >  2:39306/1‑93 (MQ=255)
                         tttGTTCGGCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGc                                                           >  1:649259/1‑93 (MQ=255)
                         tttGTTCGGCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGc                                                           <  1:5894281/93‑1 (MQ=255)
                          ttGTTCGGCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGcc                                                          >  1:5581046/1‑93 (MQ=255)
                            gTTCGGCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCAt                                                        <  1:2252531/93‑1 (MQ=255)
                            gTTCGGCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCAt                                                        <  2:6163587/93‑1 (MQ=255)
                             ttCGGCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATc                                                       <  1:3400723/93‑1 (MQ=255)
                               cGGCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCaa                                                     <  2:5764935/93‑1 (MQ=255)
                               cGGCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCaa                                                     <  2:7014463/93‑1 (MQ=255)
                                ggCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAAt                                                    <  2:3659547/93‑1 (MQ=255)
                                ggCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAAt                                                    >  2:2423914/1‑93 (MQ=255)
                                ggCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAAt                                                    <  1:5281242/93‑1 (MQ=255)
                                 gCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATa                                                   <  2:3153791/93‑1 (MQ=255)
                                 gCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATa                                                   <  1:621186/93‑1 (MQ=255)
                                   aTCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAg                                                 <  1:1166636/93‑1 (MQ=255)
                                    tCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGc                                                >  2:1926453/1‑93 (MQ=255)
                                     cTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCt                                               >  2:5325297/1‑93 (MQ=255)
                                      ttGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTc                                              <  2:1079619/93‑1 (MQ=255)
                                      ttGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTc                                              <  2:4775695/93‑1 (MQ=255)
                                      ttGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTc                                              >  2:3415993/1‑93 (MQ=255)
                                      ttGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTc                                              >  2:3367242/1‑93 (MQ=255)
                                      ttGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTc                                              <  2:2778614/93‑1 (MQ=255)
                                       tGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCg                                             <  1:2732120/93‑1 (MQ=255)
                                          ggcgCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGcn                                          >  2:2895045/1‑92 (MQ=255)
                                          ggcgCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCg                                          <  2:5266496/93‑1 (MQ=255)
                                            cgcACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTa                                        <  2:4110181/93‑1 (MQ=255)
                                             gcACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAg                                       >  1:7013514/1‑93 (MQ=255)
                                              cACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGc                                      <  1:2948136/93‑1 (MQ=255)
                                               aCCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCt                                     <  1:4098471/93‑1 (MQ=255)
                                               aCCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCt                                     >  1:3927835/1‑93 (MQ=255)
                                               aCCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCt                                     >  1:1379462/1‑93 (MQ=255)
                                                 cgcgGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTAc                                   <  2:4291010/93‑1 (MQ=255)
                                                 cgcgGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTAc                                   <  2:5021826/93‑1 (MQ=255)
                                                  gcggcgTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTAcc                                  >  2:1757996/1‑93 (MQ=255)
                                                     gcgTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAAt                               <  1:4383109/93‑1 (MQ=255)
                                                      cgTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAAtt                              <  1:6131719/93‑1 (MQ=255)
                                                       gTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTg                             >  1:2181400/1‑93 (MQ=255)
                                                       gTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTg                             <  2:1373514/93‑1 (MQ=255)
                                                        ttCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTgg                            >  2:652536/1‑93 (MQ=255)
                                                        ttCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTgg                            >  2:7446638/1‑93 (MQ=255)
                                                        ttCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTgg                            >  2:1284932/1‑93 (MQ=255)
                                                        ttCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTgg                            <  1:3699260/93‑1 (MQ=255)
                                                         tCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGc                           >  2:4329258/1‑93 (MQ=255)
                                                         tCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGc                           >  2:2294232/1‑93 (MQ=255)
                                                            ccGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCAcc                        <  2:5506901/93‑1 (MQ=255)
                                                            ccGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCAcc                        >  2:4002077/1‑93 (MQ=255)
                                                              gcgAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTg                      >  1:3547597/1‑93 (MQ=255)
                                                               cgAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGa                     <  1:77752/93‑1 (MQ=255)
                                                                 aataatCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGACa                   >  1:7234698/1‑93 (MQ=255)
                                                                 aataatCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGACa                   >  1:1889112/1‑93 (MQ=255)
                                                                 aataatCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGACa                   >  2:6620994/1‑93 (MQ=255)
                                                                   taatCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGACATc                 >  1:7140614/1‑93 (MQ=255)
                                                                    nntCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGACATcc                <  1:3325270/91‑1 (MQ=255)
                                                                    aatCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGACATcc                >  2:903527/1‑93 (MQ=255)
                                                                    aatCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGACATcc                <  1:5092600/93‑1 (MQ=255)
                                                                     atCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGACATCCg               >  2:7362543/1‑93 (MQ=255)
                                                                     atCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGACATCCg               >  1:4600521/1‑93 (MQ=255)
                                                                       cTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGACATCCGcc             >  2:1141733/1‑93 (MQ=255)
                                                                          cGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGACATCCGCCTTc          <  1:6842343/93‑1 (MQ=255)
                                                                            aaCCGTCCGGCGCGCCGACCCGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGACATCCGCCTTCga        >  1:6683451/1‑93 (MQ=255)
                                                                            aaCCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGACATCCGCCTTCga        <  2:2605043/93‑1 (MQ=255)
                                                                            aaCCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGACATCCGCCTTCga        >  1:4264225/1‑93 (MQ=255)
                                                                            aaCCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGACATCCGCCTTCga        >  1:6002398/1‑93 (MQ=255)
                                                                               cGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGACATCCGCCTTCGAGAc     >  2:7077380/1‑93 (MQ=255)
                                                                               cGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGACATCCGCCTTCGAGAc     >  2:6032529/1‑93 (MQ=255)
                                                                                gTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGACATCCGCCTTCGAGACg    >  1:3774758/1‑93 (MQ=255)
                                                                                gTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGACATCCGCCTTCGAGACg    >  1:6319234/1‑93 (MQ=255)
                                                                                 tCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGACATCCGCCTTCGAGACgg   >  1:5507160/1‑93 (MQ=255)
                                                                                 tCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGACATCCGCCTTCGAGACgg   <  1:2066032/93‑1 (MQ=255)
                                                                                       ||                                                                                      
AGCTCTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCCGGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGACATCCGCCTTCGAGACGGG  >  NC_000913/3987499‑3987673

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 250 total aligned reads displayed.
TTCAGTAGCTCTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACC‑GCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCCGGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGACATCCGCCTTCGAGACGGGTA  >  NC_000913/3987493‑3987675
                                                                                              |                                                                                         
TTCAGTAGCTCTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACC‑GCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCG                                                                                           >  SRR4302542.7369283/1‑94 (MQ=60)
      AGCTCTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACC‑GCGGCGTTCACCGCGAATAATCTGTGAACCGTCCGGCGCGCCGA                                                                                     >  SRR4302542.1049741/1‑94 (MQ=60)
      AGCTCTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACC‑GCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGA                                                                                     >  SRR4302542.2464250/1‑94 (MQ=60)
      AGCTCTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACC‑GCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGA                                                                                     <  SRR4302542.121587/94‑1 (MQ=60)
      AGCTCTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACC‑GCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGA                                                                                     >  SRR4302542.2480287/1‑94 (MQ=60)
        CTCTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACC‑GCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACC                                                                                   >  SRR4302542.658856/1‑94 (MQ=60)
        CTCTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACC‑GCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACC                                                                                   <  SRR4302542.7288356/94‑1 (MQ=60)
        CTCTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACC‑GCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACC                                                                                   >  SRR4302542.84824/1‑94 (MQ=60)
         TCTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACC‑GCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCA                                                                                  <  SRR4302542.4075389/94‑1 (MQ=60)
         TCTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACC‑GCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCA                                                                                  >  SRR4302542.1981451/1‑94 (MQ=60)
         TCTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACC‑GCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCA                                                                                  >  SRR4302542.5607889/1‑94 (MQ=60)
          CTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACC‑GCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAG                                                                                 <  SRR4302542.4167475/94‑1 (MQ=60)
          CTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACC‑GCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAG                                                                                 >  SRR4302542.3773706/1‑94 (MQ=60)
          CTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACC‑GCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAG                                                                                 <  SRR4302542.3736073/94‑1 (MQ=60)
          CTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACC‑GCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAG                                                                                 >  SRR4302542.7243486/1‑94 (MQ=60)
           TTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACC‑GCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGC                                                                                >  SRR4302542.4911482/1‑94 (MQ=60)
           TTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACC‑GCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGC                                                                                >  SRR4302542.7459046/1‑94 (MQ=60)
           TTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACC‑GCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGC                                                                                <  SRR4302542.6115762/94‑1 (MQ=60)
            TCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACC‑GCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCG                                                                               <  SRR4302542.848541/94‑1 (MQ=60)
             CTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACC‑GCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGC                                                                              >  SRR4302542.595165/1‑94 (MQ=60)
             CTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACC‑GCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGC                                                                              >  SRR4302542.916913/1‑94 (MQ=60)
             CTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACC‑GCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGC                                                                              >  SRR4302542.5441340/1‑94 (MQ=60)
             CTGCCAGCGAAAGCCCAATTTGTTCGGCATCTTGCGGCGCCCC‑GCGGCGTTGACCGCGAATAATCTGCGATCCGTCCGGCGCGCCGACCAGCGC                                                                              <  SRR4302542.2190308/94‑1 (MQ=60)
               GCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACC‑GCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCAC                                                                            <  SRR4302542.6026032/94‑1 (MQ=60)
                CCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACC‑GCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACG                                                                           >  SRR4302542.5814565/1‑94 (MQ=60)
                 CAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACC‑GCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGC                                                                          >  SRR4302542.3127468/1‑94 (MQ=60)
                 CAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACC‑GCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGC                                                                          >  SRR4302542.1789927/1‑94 (MQ=60)
                 CAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACC‑GCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGC                                                                          <  SRR4302542.4375495/94‑1 (MQ=60)
                 CAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACC‑GCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGC                                                                          >  SRR4302542.826539/1‑94 (MQ=60)
                 CAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACC‑GCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGC                                                                          <  SRR4302542.4387529/94‑1 (MQ=60)
                  AGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACC‑GCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCA                                                                         >  SRR4302542.3742000/1‑94 (MQ=60)
                   GCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACC‑GCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAG                                                                        <  SRR4302542.5389464/94‑1 (MQ=60)
                   GCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACC‑GCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAG                                                                        <  SRR4302542.2175677/94‑1 (MQ=60)
                   GCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACC‑GCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAG                                                                        <  SRR4302542.6750072/94‑1 (MQ=60)
                    CGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACC‑GCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGC                                                                       >  SRR4302542.6031949/1‑94 (MQ=60)
                    CGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACC‑GCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGC                                                                       >  SRR4302542.1071505/1‑94 (MQ=60)
                    CGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACC‑GCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGC                                                                       >  SRR4302542.1185238/1‑94 (MQ=60)
                    CGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACC‑GCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGC                                                                       <  SRR4302542.5162731/94‑1 (MQ=60)
                    CGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACC‑GCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGC                                                                       >  SRR4302542.1804917/1‑94 (MQ=60)
                    CGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACC‑GCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGC                                                                       >  SRR4302542.2010070/1‑94 (MQ=60)
                    CGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACC‑GCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGC                                                                       >  SRR4302542.5662247/1‑94 (MQ=60)
                     GAAATCCCCATTTGTTCGGCATCTTGCGGCGCACC‑GCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCC                                                                      >  SRR4302542.1866413/1‑94 (MQ=60)
                       AATCCCCATTTGTTCGGCATCTTGCGGCGCACC‑GCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAG                                                                    >  SRR4302542.1569820/1‑94 (MQ=60)
                       AATCCCCATTTGTTCGGCATCTTGCGGCGCACC‑GCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAG                                                                    >  SRR4302542.3330960/1‑94 (MQ=60)
                       AATCCCCATTTGTTCGGCATCTTGCGGCGCACC‑GCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAG                                                                    <  SRR4302542.6906485/94‑1 (MQ=60)
                       AATCCCCATTTGTTCGGCATCTTGCGGCGCACC‑GCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAG                                                                    <  SRR4302542.5800937/94‑1 (MQ=60)
                       AATCCCCATTTGTTCGGCATCTTGCGGCGCACC‑GCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAG                                                                    >  SRR4302542.6786719/1‑94 (MQ=60)
                       AATCCCCATTTGTTCGGCATCTTGCGGCGCACC‑GCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAG                                                                    >  SRR4302542.4898145/1‑94 (MQ=60)
                        ATCCCCATTTGTTCGGCATCTTGCGGCGCACC‑GCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGA                                                                   >  SRR4302542.6662451/1‑94 (MQ=60)
                        ATCCCCATTTGTTCGGCATCTTGCGGCGCACC‑GCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGA                                                                   >  SRR4302542.1581218/1‑94 (MQ=60)
                        ATCCCCATTTGTTCGGCATCTTGCGGCGCACC‑GCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGA                                                                   >  SRR4302542.731268/1‑94 (MQ=60)
                        ATCCCCATTTGTTCGGCATCTTGCGGCGCACC‑GCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGA                                                                   >  SRR4302542.871818/1‑94 (MQ=60)
                        ATCCCCATTTGTTCGGCATCTTGCGGCGCACC‑GCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGA                                                                   >  SRR4302542.3880560/1‑94 (MQ=60)
                        ATCCCCATTTGTTCGGCATCTTGCGGCGCACC‑GCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGA                                                                   >  SRR4302542.4293373/1‑94 (MQ=60)
                         TCCCCATTTGTTCGGCATCTTGCGGCGCACC‑GCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGAT                                                                  >  SRR4302542.6738095/1‑94 (MQ=60)
                         TCCCCATTTGTTCGGCATCTTGCGGCGCACC‑GCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGAT                                                                  <  SRR4302542.3467140/94‑1 (MQ=60)
                         TCCCCATTTGTTCGGCATCTTGCGGCGCACC‑GCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGAT                                                                  <  SRR4302542.4565915/94‑1 (MQ=60)
                          CCCCATTTGTTCGGCATCTTGCGGCGCACC‑GCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATT                                                                 <  SRR4302542.5506088/94‑1 (MQ=60)
                          CCCCATTTGTTCGGCATCTTGCGGCGCACC‑GCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATT                                                                 >  SRR4302542.3671764/1‑94 (MQ=60)
                          CCCCATTTGTTCGGCATCTTGCGGCGCACC‑GCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATT                                                                 <  SRR4302542.2934642/94‑1 (MQ=60)
                          CCCCATTTGTTCGGCATCTTGCGGCGCACC‑GCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATT                                                                 >  SRR4302542.4464546/1‑94 (MQ=60)
                           CCCATTTGTTCGGCATCTTGCGGCGCACC‑GCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTT                                                                <  SRR4302542.6268474/94‑1 (MQ=60)
                           CCCATTTGTTCGGCATCTTGCGGCGCACC‑GCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTT                                                                <  SRR4302542.7195252/94‑1 (MQ=60)
                           CCCATTTGTTCGGCATCTTGCGGCGCACC‑GCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTT                                                                >  SRR4302542.7557091/1‑94 (MQ=60)
                            CCATTTGTTCGGCATCTTGCGGCGCACC‑GCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTC                                                               <  SRR4302542.6683974/94‑1 (MQ=60)
                            CCATTTGTTCGGCATCTTGCGGCGCACC‑GCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTC                                                               >  SRR4302542.4140983/1‑94 (MQ=60)
                             CATTTGTTCGGCATCTTGCGGCGCACC‑GCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCG                                                              <  SRR4302542.5461364/94‑1 (MQ=60)
                             CATTTGTTCGGCATCTTGCGGCGCACC‑GCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCG                                                              >  SRR4302542.1719602/1‑94 (MQ=60)
                             CATTTGTTCGGCATCTTGCGGCGCACC‑GCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCG                                                              >  SRR4302542.3071546/1‑94 (MQ=60)
                              atttcgataacaatcagacgaccgtttttcggGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCG                                                              >  SRR4302542.222576/33‑94 (MQ=60)
                              ATTTGTTCGGCATCTTGCGGCGCACC‑GCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGC                                                             >  SRR4302542.310267/1‑94 (MQ=60)
                              ATTTGTTCGGCATCTTGCGGCGCACC‑GCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGC                                                             >  SRR4302542.654825/1‑94 (MQ=60)
                              ATTTGTTCGGCATCTTGCGGCGCACC‑GCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGC                                                             >  SRR4302542.2116814/1‑94 (MQ=60)
                              ATTTGTTCGGCATCTTGCGGCGCACC‑GCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGC                                                             >  SRR4302542.2212648/1‑94 (MQ=60)
                               TTTGTTCGGCATCTTGCGGCGCACC‑GCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCC                                                            <  SRR4302542.5951402/94‑1 (MQ=60)
                               TTTGTTCGGCATCTTGCGGCGCACC‑GCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCC                                                            <  SRR4302542.4662240/94‑1 (MQ=60)
                               TTTGTTCGGCATCTTGCGGCGCACC‑GCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCC                                                            <  SRR4302542.3498521/94‑1 (MQ=60)
                               TTTGTTCGGCATCTTGCGGCGCACC‑GCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCC                                                            >  SRR4302542.5635274/1‑94 (MQ=60)
                               TTTGTTCGGCATCTTGCGGCGCACC‑GCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCC                                                            <  SRR4302542.4043437/94‑1 (MQ=60)
                                TTGTTCGGCATCTTGCGGCGCACC‑GCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCA                                                           >  SRR4302542.3262304/1‑94 (MQ=60)
                                TTGTTCGGCATCTTGCGGCGCACC‑GCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCA                                                           >  SRR4302542.5563028/1‑94 (MQ=60)
                                TTGTTCGGCATCTTGCGGCGCACC‑GCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAAATTTCGCCA                                                           <  SRR4302542.1077198/94‑1 (MQ=60)
                                 TGTTCGGCATCTTGCGGCGCACC‑GCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCAT                                                          >  SRR4302542.214275/1‑94 (MQ=60)
                                 TGTTCGGCATCTTGCGGCGCACC‑GCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCAT                                                          >  SRR4302542.7216607/1‑94 (MQ=60)
                                  GTTCGGCATCTTGCGGCGCACC‑GCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATC                                                         <  SRR4302542.2271908/94‑1 (MQ=60)
                                   TTCGGCATCTTGCGGCGCACC‑GCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCA                                                        <  SRR4302542.6691260/94‑1 (MQ=60)
                                   TTCGGCATCTTGCGGCGCACC‑GCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCA                                                        >  SRR4302542.2312837/1‑94 (MQ=60)
                                    TCGGCATCTTGCGGCGCACCGGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCA                                                        >  SRR4302542.6205189/1‑94 (MQ=60)
                                    TCGGCATCTTGCGGCGCACC‑GCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAA                                                       <  SRR4302542.4653198/94‑1 (MQ=60)
                                    TCGGCATCTTGCGGCGCACC‑GCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAA                                                       >  SRR4302542.6923932/1‑94 (MQ=60)
                                    TCGGCATCTTGCGGCGCACC‑GCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAA                                                       <  SRR4302542.3836943/94‑1 (MQ=60)
                                    TCGGCATCTTGCGGCGCACC‑GCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAA                                                       <  SRR4302542.714683/94‑1 (MQ=60)
                                      GGCATCTTGCGGCGCACC‑GCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATA                                                     <  SRR4302542.5332591/94‑1 (MQ=60)
                                      GGCATCTTGCGGCGCACC‑GCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATA                                                     <  SRR4302542.1627654/94‑1 (MQ=60)
                                      GGCATCTTGCGGCGCACC‑GCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATA                                                     <  SRR4302542.7534082/94‑1 (MQ=60)
                                       GCATCTTGCGGCGCACC‑GCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAA                                                    <  SRR4302542.626530/94‑1 (MQ=60)
                                       GCATCTTGCGGCGCACC‑GCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAA                                                    <  SRR4302542.7260339/94‑1 (MQ=60)
                                        CATCTTGCGGCGCACC‑GCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAG                                                   <  SRR4302542.7175364/94‑1 (MQ=60)
                                        CATCTTGCGGCGCACC‑GCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAG                                                   >  SRR4302542.7468620/1‑94 (MQ=60)
                                         ATCTTGCGGCGCACC‑GCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGC                                                  <  SRR4302542.7364913/94‑1 (MQ=60)
                                         ATCTTGCGGCGCACC‑GCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGC                                                  <  SRR4302542.1176692/94‑1 (MQ=60)
                                         ATCTTGCGGCGCACC‑GCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGC                                                  >  SRR4302542.889377/1‑94 (MQ=60)
                                         ATCTTGCGGCGCACC‑GCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGC                                                  >  SRR4302542.6337338/1‑94 (MQ=60)
                                         ATCTTGCGGCGCACC‑GCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGC                                                  >  SRR4302542.7086220/1‑94 (MQ=60)
                                          TCTTGCGGCGCACC‑GCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCT                                                 >  SRR4302542.1432552/1‑94 (MQ=60)
                                          TCTTGCGGCGCACC‑GCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCT                                                 >  SRR4302542.6862117/1‑94 (MQ=60)
                                           CTTGCGGCGCACC‑GCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTC                                                >  SRR4302542.3659838/1‑94 (MQ=60)
                                           CTTGCGGCGCACC‑GCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTC                                                >  SRR4302542.1309653/1‑94 (MQ=60)
                                           CTTGCGGCGCACC‑GCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTC                                                >  SRR4302542.6510396/1‑94 (MQ=60)
                                           CTTGCGGCGCACC‑GCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTC                                                <  SRR4302542.839165/94‑1 (MQ=60)
                                            TTGCGGCGCACC‑GCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCG                                               <  SRR4302542.6174696/94‑1 (MQ=60)
                                            TTGCGGCGCACC‑GCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCG                                               <  SRR4302542.6174695/94‑1 (MQ=60)
                                            TTGCGGCGCACC‑GCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCG                                               <  SRR4302542.1960347/94‑1 (MQ=60)
                                             TGCGGCGCACC‑GCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGG                                              <  SRR4302542.2758481/94‑1 (MQ=60)
                                               CGGCGCACC‑GCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCG                                            <  SRR4302542.4402638/94‑1 (MQ=60)
                                               CGGCGCACC‑GCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCG                                            >  SRR4302542.1177181/1‑94 (MQ=60)
                                               CGGCGCACC‑GCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCG                                            >  SRR4302542.1214480/1‑94 (MQ=60)
                                                 GCGCACC‑GCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTA                                          <  SRR4302542.87789/94‑1 (MQ=60)
                                                  CGCACC‑GCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAG                                         <  SRR4302542.6746446/94‑1 (MQ=60)
                                                  CGCACC‑GCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAG                                         <  SRR4302542.306648/94‑1 (MQ=60)
                                                  CGCACC‑GCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAG                                         >  SRR4302542.5342935/1‑94 (MQ=60)
                                                  CGCACC‑GCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAG                                         <  SRR4302542.6365336/94‑1 (MQ=60)
                                                    CACC‑GCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCT                                       <  SRR4302542.2976362/94‑1 (MQ=60)
                                                    CACC‑GCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCT                                       >  SRR4302542.2246276/1‑94 (MQ=60)
                                                    CACC‑GCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCT                                       >  SRR4302542.94845/1‑94 (MQ=60)
                                                    CACC‑GCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCT                                       >  SRR4302542.1391385/1‑94 (MQ=60)
                                                    CACC‑GCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCT                                       >  SRR4302542.3966591/1‑94 (MQ=60)
                                                     ACC‑GCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTA                                      >  SRR4302542.5564671/1‑94 (MQ=60)
                                                     ACC‑GCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTA                                      <  SRR4302542.4138893/94‑1 (MQ=60)
                                                     ACC‑GCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTA                                      >  SRR4302542.1371671/1‑94 (MQ=60)
                                                     ACC‑GCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTA                                      >  SRR4302542.6242385/1‑94 (MQ=60)
                                                      CC‑GCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTAC                                     >  SRR4302542.6405104/1‑94 (MQ=60)
                                                       C‑GCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACC                                    >  SRR4302542.81475/1‑94 (MQ=60)
                                                         GCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCA                                   <  SRR4302542.2270427/94‑1 (MQ=60)
                                                          CGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAA                                  >  SRR4302542.3493013/1‑94 (MQ=60)
                                                           GGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAAT                                 <  SRR4302542.95159/94‑1 (MQ=60)
                                                           GGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAAT                                 <  SRR4302542.6299626/94‑1 (MQ=60)
                                                           GGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAAT                                 >  SRR4302542.1546742/1‑94 (MQ=60)
                                                            GCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATT                                <  SRR4302542.4426123/94‑1 (MQ=60)
                                                            GCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATT                                <  SRR4302542.6439515/94‑1 (MQ=60)
                                                            GCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATT                                <  SRR4302542.3428957/94‑1 (MQ=60)
                                                             CGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTG                               <  SRR4302542.6191037/94‑1 (MQ=60)
                                                             CGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTG                               >  SRR4302542.2200186/1‑94 (MQ=60)
                                                             CGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTG                               <  SRR4302542.5227878/94‑1 (MQ=60)
                                                             CGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTG                               >  SRR4302542.5854745/1‑94 (MQ=60)
                                                              GTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGG                              >  SRR4302542.5654828/1‑94 (MQ=60)
                                                              GTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGG                              >  SRR4302542.4567150/1‑94 (MQ=60)
                                                              GTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGG                              >  SRR4302542.5140702/1‑94 (MQ=60)
                                                              GTTCACAGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGG                              >  SRR4302542.5341222/1‑94 (MQ=60)
                                                               TTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGC                             <  SRR4302542.3735865/94‑1 (MQ=60)
                                                                TCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCA                            >  SRR4302542.694723/1‑94 (MQ=60)
                                                                TCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCA                            <  SRR4302542.2950922/94‑1 (MQ=60)
                                                                 CACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCAC                           <  SRR4302542.6632531/94‑1 (MQ=60)
                                                                 CACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCAC                           >  SRR4302542.5327408/1‑94 (MQ=60)
                                                                   CCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCT                         >  SRR4302542.7330877/1‑94 (MQ=60)
                                                                   CCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCT                         <  SRR4302542.4116924/94‑1 (MQ=60)
                                                                    CGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTG                        >  SRR4302542.3580765/1‑94 (MQ=60)
                                                                      CGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGAC                      <  SRR4302542.7023644/94‑1 (MQ=60)
                                                                      CGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGAC                      >  SRR4302542.3315538/1‑94 (MQ=60)
                                                                      CGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGAC                      <  SRR4302542.7355242/94‑1 (MQ=60)
                                                                      CGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGAC                      <  SRR4302542.78402/94‑1 (MQ=60)
                                                                       GAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGACA                     >  SRR4302542.1905461/1‑94 (MQ=60)
                                                                       GAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGACA                     >  SRR4302542.3422600/1‑94 (MQ=60)
                                                                       GAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGACA                     >  SRR4302542.3489233/1‑94 (MQ=60)
                                                                        AATAATCTGCTAGCCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCCCCTGACAT                    <  SRR4302542.3583523/94‑1 (MQ=60)
                                                                        AATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGACAT                    >  SRR4302542.5546297/1‑94 (MQ=60)
                                                                          TAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGACATCC                  >  SRR4302542.7310559/1‑94 (MQ=60)
                                                                          TAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGACATCC                  <  SRR4302542.3868790/94‑1 (MQ=60)
                                                                          TAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGACATCC                  <  SRR4302542.6961872/94‑1 (MQ=60)
                                                                           NNTCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGACATCCG                 <  SRR4302542.3356583/94‑1 (MQ=60)
                                                                           AATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGACATCCG                 <  SRR4302542.4935178/94‑1 (MQ=60)
                                                                           AATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGACATCCG                 <  SRR4302542.5142090/94‑1 (MQ=60)
                                                                           AATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGACATCCG                 >  SRR4302542.4645493/1‑94 (MQ=60)
                                                                           AATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGACATCCG                 >  SRR4302542.3422006/1‑94 (MQ=60)
                                                                            ATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGACATCCGC                >  SRR4302542.5900797/1‑94 (MQ=60)
                                                                            ATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGACATCCGC                >  SRR4302542.1228595/1‑94 (MQ=60)
                                                                              CTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGACATCCGCCT              <  SRR4302542.2667675/94‑1 (MQ=60)
                                                                               TGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGACATCCGCCTT             <  SRR4302542.1898919/94‑1 (MQ=60)
                                                                               TGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGACATCCGCCTT             <  SRR4302542.7313318/94‑1 (MQ=60)
                                                                               TGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGACATCCGCCTT             >  SRR4302542.7402943/1‑94 (MQ=60)
                                                                                  GAACCGTCCGGCGCGCCGACCCGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGACATCCGCCTTCGA          >  SRR4302542.6749281/1‑94 (MQ=60)
                                                                                  GAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGACATCCGCCTTCGA          >  SRR4302542.4306196/1‑94 (MQ=60)
                                                                                  GAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGACATCCGCCTTCGA          >  SRR4302542.6060532/1‑94 (MQ=60)
                                                                                   AACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGACATCCGCCTTCGAG         >  SRR4302542.2881186/1‑94 (MQ=60)
                                                                                    ACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGACATCCGCCTTCGAGA        >  SRR4302542.1417286/1‑94 (MQ=60)
                                                                                     CCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGACATCCGCCTTCGAGAC       >  SRR4302542.484310/1‑94 (MQ=60)
                                                                                      CGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGACATCCGCCTTCGAGACG      >  SRR4302542.1104280/1‑94 (MQ=60)
                                                                                      CGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGACATCCGCCTTCGAGACG      >  SRR4302542.3812096/1‑94 (MQ=60)
                                                                                      CGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGACATCCGCCTTCGAGACG      <  SRR4302542.47074/94‑1 (MQ=60)
                                                                                      CGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGACATCCGCCTTCGAGACG      >  SRR4302542.6381515/1‑94 (MQ=60)
                                                                                      CGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGACATCCGCCTTCGAGACG      >  SRR4302542.569729/1‑94 (MQ=60)
                                                                                      CGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGACATCCGCCTTCGAGACG      <  SRR4302542.580330/94‑1 (MQ=60)
                                                                                       GTCCGGCGCGCCGACCAGCGCACGCAGCCGGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGACATCCGCCTTCGAGACGG     >  SRR4302542.1165973/1‑94 (MQ=60)
                                                                                       GTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGACATCCGCCTTCGAGACGG     >  SRR4302542.5560679/1‑94 (MQ=60)
                                                                                        TCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGACATCCGCCTTCGAGACGGG    <  SRR4302542.2672734/94‑1 (MQ=60)
                                                                                        TCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGACATCCGCCTTCGAGACGGG    <  SRR4302542.2083895/94‑1 (MQ=60)
                                                                                         CCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGACATCCGCCTTCGAGACGGGT   <  SRR4302542.2434559/94‑1 (MQ=60)
                                                                                         CCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGACATCCGCCTTCGAGACGGGT   >  SRR4302542.6835980/1‑94 (MQ=60)
                                                                                          CGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGACATCCGCCTTCGAGACGGGTA  >  SRR4302542.2728267/1‑94 (MQ=60)
                                                                                          CGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGACATCCGCCTTCGAGACGGGTA  <  SRR4302542.3424233/94‑1 (MQ=60)
                                                                                              |                                                                                         
TTCAGTAGCTCTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACC‑GCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCCGGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGACATCCGCCTTCGAGACGGGTA  >  NC_000913/3987493‑3987675

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑