Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1061 294.1 15065038 98.6% 14854127 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,008,491 2 bp→CG intergenic (+58/‑174) b4555 → / → yigM hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,008,4910GC99.7% 1241.1 / NA 370intergenic (+58/‑175)b4555/yigMhypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base C (171/198);  total (171/199)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*NC_0009134,008,4920CG99.5% 1224.9 / NA 365intergenic (+59/‑174)b4555/yigMhypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (0/2);  new base G (166/197);  total (166/199)
Fisher's exact test for biased strand distribution p-value = 5.03e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 370 total aligned reads displayed.

CTGAAGGATTG‑ATTGCGTT‑‑GTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGGAAA‑GCTGCATTATGGGCAGTGGCCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑GAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACT  >  NC_000913/4008411‑4008577
                                                                                     ||                                                                                      
cTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGGAAAGGCTGCATTATGGGCAGTGCGCACCAg                                                                                  >  2:1382978/1‑93 (MQ=255)
 tGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGGAAAGGCTGCATTATGGGCAGTGCGCACCAGc                                                                                 <  1:1202663/93‑1 (MQ=255)
 tGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGGAAAGGCTGCATTATGGGCAGTGCGCACCAGc                                                                                 >  2:6209325/1‑93 (MQ=255)
 tGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGGAAAGGCTGCATTATGGGCAGTGCGCACCAGc                                                                                 >  1:7078464/1‑93 (MQ=255)
 tGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGGAAAGGCTGCATTATGGGCAGTGCGCACCAGc                                                                                 >  2:5455356/1‑93 (MQ=255)
 tGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGGAAAGGCTGCATTATGGGCAGTGCGCACCAGc                                                                                 <  1:209109/93‑1 (MQ=255)
 tGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGGAAAGGCTGCATTATGGGCAGTGCGCACCAGc                                                                                 <  2:1959311/93‑1 (MQ=255)
  gAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGGAAAGGCTGCTTTATGGGCAGTGCGCACCAGCg                                                                                >  1:1112201/1‑93 (MQ=255)
  gAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCg                                                                                >  2:1138424/1‑93 (MQ=255)
    aGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTc                                                                              >  2:3427937/1‑93 (MQ=255)
    aGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTc                                                                              <  2:471445/93‑1 (MQ=255)
                  tttggtgACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCAt                                                                >  2:6948155/5‑93 (MQ=255)
                  tttggtgACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCAt                                                                >  2:2939129/5‑93 (MQ=255)
                    tggtgACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATcc                                                              <  1:2764591/91‑1 (MQ=255)
                       tgACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGa                                                           <  1:5312574/93‑1 (MQ=255)
                        gACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAg                                                          <  2:1858739/93‑1 (MQ=255)
                           gggATGAACGACGCCGAAATGCTGTCGATGGCGGGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑g                                                      >  1:4515664/1‑93 (MQ=255)
                             gATGAACGACGCCGAAATGCTGTCGATGGCGGGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑gga                                                    >  1:3510725/1‑91 (MQ=255)
                              aTGAACGACGCCGAAATGCTGTCGATGGCGGGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑ggaa                                                   <  2:4884585/93‑4 (MQ=255)
                              aTGAACGACGCCGAAATGCTGTCGATGGCGGGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑ggaa                                                   <  1:273441/93‑4 (MQ=255)
                              aTGAACGACGCCGAAATGCTGTCGATGGCGGGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑ggaa                                                   <  1:2399878/93‑4 (MQ=255)
                               tGAACGACGCCGAAATGCTGTCGATGGCGGGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑ggaag                                                  >  1:447193/1‑89 (MQ=255)
                                 aaCGACGCCGAAATGCTGTCGATGGCGGGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTg                                                 >  2:750328/1‑93 (MQ=255)
                                  acgacgCCGAAATGCTGTCGATGGCGGGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGa                                                <  2:2110599/93‑1 (MQ=255)
                                  acgacgCCGAAATGCTGTCGATGGCGGGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGa                                                >  1:7429522/1‑93 (MQ=255)
                                   cgacgCCGAAATGCTGTCGATGGCGGGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGAt                                               <  2:3815664/93‑1 (MQ=255)
                                   cgacgCCGAAATGCTGTCGATGGCGGGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGAt                                               <  2:2887079/93‑1 (MQ=255)
                                     acgCCGAAATGCTGTCGATGGCGGGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTg                                             >  1:1363953/1‑93 (MQ=255)
                                     acgCCGAAATGCTGTCGATGGCGGGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTg                                             >  2:3238232/1‑93 (MQ=255)
                                       gCCGAAATGCTGTCGATGGCGGGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGt                                           <  1:719952/93‑1 (MQ=255)
                                        ccGAAATGCTGTCGATGGCGGGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTa                                          <  2:5608195/93‑1 (MQ=255)
                                        ccGAAATGCTGTCGATGGCGGGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTa                                          <  2:248902/93‑1 (MQ=255)
                                         cGAAATGCTGTCGATGGCGGGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTAc                                         >  1:5644213/1‑93 (MQ=255)
                                         cGAAATGCTGTCGATGGCGGGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTAc                                         <  2:7378296/93‑1 (MQ=255)
                                         cGAAATGCTGTCGATGGCGGGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTAc                                         <  1:3942131/93‑1 (MQ=255)
                                          gagaTGCTGTCGATGGCGGGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACt                                        <  1:5861038/90‑1 (MQ=255)
                                          gAAATGCTGTCGATGGCGGGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACt                                        >  2:150043/1‑93 (MQ=255)
                                           aaaTGCTGTCGATGGCGGGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTa                                       <  1:2436251/93‑1 (MQ=255)
                                            aaTGCTGTCGATGGCGGGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTaa                                      <  2:4088021/93‑1 (MQ=255)
                                            aaTGCTGTCGATGGCGGGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTaa                                      >  2:6796432/1‑93 (MQ=255)
                                            aaTGCTGTCGATGGCGGGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTaa                                      <  1:966786/93‑1 (MQ=255)
                                            aaTGCTGTCGATGGCGGGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTaa                                      <  1:414147/93‑1 (MQ=255)
                                             aTGCTGTCGATGGCGGGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAAt                                     <  1:3754984/93‑1 (MQ=255)
                                              tGCTGTCGATGGCGGGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATg                                    >  2:4941722/1‑93 (MQ=255)
                                              tGCTGTCGATGGCGGGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATg                                    <  1:6470282/93‑1 (MQ=255)
                                              tGCTGTCGATGGCGGGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATg                                    >  2:3168847/1‑93 (MQ=255)
                                               gCTGTCGATGGCGGGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGGGCTGGAAGTGATTGGTACTAATGc                                   <  1:659224/93‑1 (MQ=255)
                                               gCTGTCGATGGCGGGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGGGCTGGAAGTGATTGGTACTAATGc                                   <  2:726571/93‑1 (MQ=255)
                                               gCTGTCGATGGCGGGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGc                                   <  1:517814/93‑1 (MQ=255)
                                                 tGTCGATGGCGGGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCcg                                 >  2:6077482/1‑93 (MQ=255)
                                                  gTCGATGGCGGGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCcga                                <  1:3119614/93‑1 (MQ=255)
                                                  gTCGATGGCGGGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCcga                                <  2:3631821/93‑1 (MQ=255)
                                                  gTCGATGGCGGGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCcga                                <  2:6870522/93‑1 (MQ=255)
                                                   tCGATGGCGGGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCcgac                               <  2:1086426/93‑1 (MQ=255)
                                                   tCGATGGCGGGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCcgac                               >  1:6710388/1‑93 (MQ=255)
                                                   tCGATGGCGGGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCcgac                               <  2:7208058/93‑1 (MQ=255)
                                                   tCGATGGCGGGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCcgac                               <  2:6492572/93‑1 (MQ=255)
                                                   tCGATGGCGGGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCcgac                               <  1:3602871/93‑1 (MQ=255)
                                                     gATGGCGGGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCcgacga                             >  1:1777695/1‑93 (MQ=255)
                                                     gATGGCGGGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCcgacga                             <  2:6032356/93‑1 (MQ=255)
                                                      aTGGCGGGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCgacgac                            >  2:6328395/1‑93 (MQ=255)
                                                      aTGGCGGGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCgacgac                            >  1:5502038/1‑93 (MQ=255)
                                                        ggcggggAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGAcgc                          <  1:6057983/89‑1 (MQ=255)
                                                          cggggAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCgg                        <  1:4896224/91‑1 (MQ=255)
                                                           ggggAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGt                       <  1:755291/92‑1 (MQ=255)
                                                           ggggAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGt                       >  2:6118904/2‑93 (MQ=255)
                                                             ggAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGc                     <  2:5218696/93‑1 (MQ=255)
                                                                aaggCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGc                  >  1:1685486/4‑93 (MQ=255)
                                                                  ggCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCAt                <  1:3907554/92‑1 (MQ=255)
                                                                  ggCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCAt                >  1:160993/2‑93 (MQ=255)
                                                                    cTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGGGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTa              <  1:902764/93‑1 (MQ=255)
                                                                    cTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTa              >  1:2938991/1‑93 (MQ=255)
                                                                     tGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGGGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTAt             <  1:6248073/93‑1 (MQ=255)
                                                                     tGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTAt             >  2:4176554/1‑93 (MQ=255)
                                                                     tGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTAt             <  2:76525/93‑1 (MQ=255)
                                                                     tGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTAt             <  1:427396/93‑1 (MQ=255)
                                                                     tGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTAt             <  1:5237510/93‑1 (MQ=255)
                                                                      gCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATc            <  2:3334585/93‑1 (MQ=255)
                                                                      gCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATc            <  2:4345596/93‑1 (MQ=255)
                                                                      gCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATc            >  1:1814614/1‑93 (MQ=255)
                                                                      gCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATc            <  2:2778616/93‑1 (MQ=255)
                                                                       cATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCt           <  2:5767344/93‑1 (MQ=255)
                                                                       cATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCt           <  1:931612/93‑1 (MQ=255)
                                                                        aTTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGGCGACGCGGTGCCGCATTATCTg          >  2:2427925/1‑93 (MQ=255)
                                                                        aTTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTg          >  1:2189860/1‑93 (MQ=255)
                                                                         ttATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGc         >  1:5429835/1‑93 (MQ=255)
                                                                         ttATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGc         >  1:3248900/1‑93 (MQ=255)
                                                                         ttATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGc         <  1:3392610/93‑1 (MQ=255)
                                                                          tATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCg        <  2:3938331/93‑1 (MQ=255)
                                                                          tATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCg        <  1:5772837/93‑1 (MQ=255)
                                                                           aTGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGt       <  2:1821233/93‑1 (MQ=255)
                                                                            tGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTa      >  2:2171423/1‑93 (MQ=255)
                                                                             gggCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTaa     <  1:6778698/93‑1 (MQ=255)
                                                                              ggCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTaaa    <  1:2578335/93‑1 (MQ=255)
                                                                              ggCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTaaa    <  1:809081/93‑1 (MQ=255)
                                                                              ggCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTaaa    >  2:1339807/1‑93 (MQ=255)
                                                                               gCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAAc   <  2:1169844/93‑1 (MQ=255)
                                                                               gCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAAc   <  1:1574299/93‑1 (MQ=255)
                                                                               gCAGTGCGCACCAGCGGCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAAc   <  1:4742218/93‑1 (MQ=255)
                                                                                cAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAAct  <  2:4489685/93‑1 (MQ=255)
                                                                                     ||                                                                                      
CTGAAGGATTG‑ATTGCGTT‑‑GTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGGAAA‑GCTGCATTATGGGCAGTGGCCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑GAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACT  >  NC_000913/4008411‑4008577

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 240 total aligned reads displayed.
GCTACAG‑CTGAAGGATTG‑ATTGCGTT‑‑GTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑G‑GG‑‑AAA‑GCTGCATTATGGGCAGTGGCCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑GAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCT  >  NC_000913/4008404‑4008579
                                                                                                 |                                                                                         
GCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑G‑GGGAAAG‑GCTGCATTATGGGCAGTGC                                                                                           >  SRR4302542.1069214/1‑94 (MQ=60)
GCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑G‑GGGAAAG‑GCTGCATTATGGGCAGTGC                                                                                           >  SRR4302542.6482249/1‑94 (MQ=60)
GCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑G‑GGGAAAG‑GCTGCATTATGGGCAGTGC                                                                                           <  SRR4302542.4742817/94‑1 (MQ=60)
     agcCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑G‑GGGAAAG‑GCTGCATTATGGGCAGTGCGCACC                                                                                      >  SRR4302542.3370650/4‑94 (MQ=60)
     agcCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑G‑GGGAAAG‑GCTGCATTATGGGCAGTGCGCACC                                                                                      >  SRR4302542.5479179/4‑94 (MQ=60)
      gcCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑G‑GGGAAAG‑GCTGCATTATGGGCAGTGCGCACCA                                                                                     <  SRR4302542.2549671/92‑1 (MQ=60)
      gcCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑G‑GGGAAAG‑GCTGCATTATGGGCAGTGCGCACCA                                                                                     <  SRR4302542.6692097/92‑1 (MQ=60)
      C‑CTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑G‑GGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAG                                                                                    <  SRR4302542.5514875/94‑1 (MQ=60)
      C‑CTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑G‑GGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAG                                                                                    >  SRR4302542.189463/1‑94 (MQ=60)
      C‑CTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑G‑GGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAG                                                                                    <  SRR4302542.3740155/94‑1 (MQ=60)
      C‑CTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑G‑GGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAG                                                                                    >  SRR4302542.5943100/1‑94 (MQ=60)
      C‑CTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑G‑GGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAG                                                                                    >  SRR4302542.6322257/1‑94 (MQ=60)
        CTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑G‑GGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGC                                                                                   >  SRR4302542.6678308/1‑94 (MQ=60)
        CTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑G‑GGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGC                                                                                   >  SRR4302542.6816858/1‑94 (MQ=60)
        CTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑G‑GGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGC                                                                                   >  SRR4302542.2781894/1‑94 (MQ=60)
        CTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑G‑GGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGC                                                                                   >  SRR4302542.4084108/1‑94 (MQ=60)
        CTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑G‑GGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGC                                                                                   >  SRR4302542.7147933/1‑94 (MQ=60)
         TGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑G‑GGGAAAG‑GCTGCTTTATGGGCAGTGCGCACCAGCG                                                                                  >  SRR4302542.1121713/1‑94 (MQ=46)
         TGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑G‑GGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCG                                                                                  <  SRR4302542.210912/94‑1 (MQ=57)
           AAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑G‑GGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTC                                                                                >  SRR4302542.5302665/1‑94 (MQ=57)
            AGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑G‑GGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCT                                                                               <  SRR4302542.4877741/94‑1 (MQ=57)
            AGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑G‑GGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCT                                                                               >  SRR4302542.2293111/1‑94 (MQ=57)
            AGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑G‑GGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCT                                                                               <  SRR4302542.3028435/94‑1 (MQ=57)
             GGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑G‑GGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTG                                                                              <  SRR4302542.2809358/94‑1 (MQ=60)
              GATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑G‑GGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGA                                                                             >  SRR4302542.268975/1‑94 (MQ=60)
               ATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑G‑GGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAA                                                                            <  SRR4302542.5287847/94‑1 (MQ=60)
               ATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑G‑GGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAA                                                                            >  SRR4302542.2816452/1‑94 (MQ=60)
               ATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑G‑GGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAA                                                                            >  SRR4302542.2307221/1‑94 (MQ=60)
               ATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑G‑GGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAA                                                                            >  SRR4302542.6961209/1‑94 (MQ=60)
                TTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑G‑GGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAA                                                                           >  SRR4302542.436692/1‑94 (MQ=60)
                 tgtattgcgtttgGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑G‑GGGAAAG‑GCTGCCTTATGGGCAGTGCGCACCAGCGTCTGCAAG                                                                          >  SRR4302542.2110417/14‑94 (MQ=55)
                 tgtattgcgtttgGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑G‑GGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAG                                                                          <  SRR4302542.1671598/81‑1 (MQ=60)
                 tgtattgcgtttgGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑G‑GGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAG                                                                          <  SRR4302542.3722288/81‑1 (MQ=60)
                 tgtattgcgtttgGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑G‑GGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAG                                                                          <  SRR4302542.4193504/81‑1 (MQ=60)
                 tgtattgcgtttgGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑G‑GGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAG                                                                          <  SRR4302542.7140634/81‑1 (MQ=60)
                 tgtattgcgtttgGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑G‑GGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAG                                                                          >  SRR4302542.7201653/14‑94 (MQ=60)
                 tgtattgcgtttgGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑G‑GGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAG                                                                          <  SRR4302542.6057393/81‑1 (MQ=60)
                  gtattgcgtttgGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑G‑GGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGA                                                                         >  SRR4302542.896807/13‑94 (MQ=60)
                  gtattgcgtttgGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑G‑GGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGA                                                                         >  SRR4302542.3204566/13‑94 (MQ=60)
                  T‑ATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑G‑GGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGAC                                                                        <  SRR4302542.3084985/94‑1 (MQ=60)
                    ATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑G‑GGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACC                                                                       <  SRR4302542.7463958/94‑1 (MQ=60)
                     TTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑G‑GGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCT                                                                      <  SRR4302542.6472354/94‑1 (MQ=60)
                     TTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑G‑GGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCT                                                                      <  SRR4302542.70354/94‑1 (MQ=60)
                     TTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑G‑GGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCT                                                                      <  SRR4302542.7354813/94‑1 (MQ=60)
                      TGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑G‑GGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTT                                                                     <  SRR4302542.2734144/94‑1 (MQ=60)
                      TGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑G‑GGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTT                                                                     <  SRR4302542.6244115/94‑1 (MQ=60)
                      TGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑G‑GGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTT                                                                     <  SRR4302542.1268330/94‑1 (MQ=60)
                       GCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑G‑GGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTC                                                                    <  SRR4302542.1747980/94‑1 (MQ=60)
                       GCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑G‑GGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTC                                                                    >  SRR4302542.6560030/1‑94 (MQ=60)
                        CGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑G‑GGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCA                                                                   >  SRR4302542.6591525/1‑94 (MQ=60)
                         gtttgGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑G‑GGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCAT                                                                  <  SRR4302542.5020400/89‑1 (MQ=60)
                         gtttgGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑G‑GGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCAT                                                                  >  SRR4302542.2556385/6‑94 (MQ=60)
                         gtttgGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑G‑GGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCAT                                                                  >  SRR4302542.925178/6‑94 (MQ=60)
                          tttgGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑G‑GGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATC                                                                 <  SRR4302542.2974286/90‑1 (MQ=60)
                          TG‑‑GTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑G‑GGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCC                                                               <  SRR4302542.2791231/94‑1 (MQ=60)
                          TG‑‑GTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑G‑GGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCC                                                               >  SRR4302542.771545/1‑94 (MQ=60)
                           G‑‑GTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑G‑GGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCG                                                              <  SRR4302542.6149693/94‑1 (MQ=60)
                           G‑‑GTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑G‑GGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCG                                                              >  SRR4302542.1608278/1‑94 (MQ=60)
                              GTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑G‑GGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGA                                                             >  SRR4302542.2514554/1‑94 (MQ=60)
                               TGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑G‑GGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGGG                                                            <  SRR4302542.766444/94‑1 (MQ=60)
                               TGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑G‑GGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAG                                                            <  SRR4302542.3680233/94‑1 (MQ=60)
                               TGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑G‑GGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAG                                                            <  SRR4302542.5364190/94‑1 (MQ=60)
                                  CGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑G‑GGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑G                                                        >  SRR4302542.117013/1‑94 (MQ=60)
                                  AGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑G‑GGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑G                                                        >  SRR4302542.4559914/1‑94 (MQ=60)
                                    GGATGAACGACGCCGAAATGCTGTCGATGGC‑‑G‑GGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑GGA                                                      >  SRR4302542.3543542/1‑94 (MQ=60)
                                    GGATGAACGACGCCGAAATGCTGTCGATGGC‑‑G‑GGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑GGA                                                      <  SRR4302542.3966224/94‑1 (MQ=60)
                                    GGATGAACGACGCCGAAATGCTGTCGATGGC‑‑G‑GGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑GGA                                                      >  SRR4302542.89664/1‑94 (MQ=60)
                                      ATGAACGACGCCGAAATGCTGTCGATGGC‑‑G‑GGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAG                                                     >  SRR4302542.5788910/1‑94 (MQ=60)
                                      ATGAACGACGCCGAAATGCTGTCGATGGC‑‑G‑GGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAG                                                     >  SRR4302542.451061/1‑94 (MQ=60)
                                      ATGAACGACGCCGAAATGCTGTCGATGGC‑‑G‑GGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAG                                                     <  SRR4302542.275784/94‑1 (MQ=60)
                                      ATGAACGACGCCGAAATGCTGTCGATGGC‑‑G‑GGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAG                                                     <  SRR4302542.2423220/94‑1 (MQ=60)
                                       TGAACGACGCCGAAATGCTGTCGATGGC‑‑G‑GGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGT                                                    <  SRR4302542.127132/94‑1 (MQ=60)
                                        GAACGACGCCGAAATGCTGTCGATGGC‑‑G‑GGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTG                                                   <  SRR4302542.1149863/94‑1 (MQ=60)
                                        GAACGACGCCGAAATGCTGTCGATGGC‑‑G‑GGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTG                                                   >  SRR4302542.1898208/1‑94 (MQ=60)
                                         AACGACGCCGAAATGCTGTCGATGGC‑‑G‑GGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGGGCTGGAAGTGA                                                  >  SRR4302542.4965141/1‑94 (MQ=60)
                                         AACGACGCCGAAATGCTGTCGATGGC‑‑G‑GGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGA                                                  >  SRR4302542.7502225/1‑94 (MQ=60)
                                          ACGACGCCGAAATGCTGTCGATGGC‑‑G‑GGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGAT                                                 <  SRR4302542.6496147/94‑1 (MQ=60)
                                          ACGACGCCGAAATGCTGTCGATGGC‑‑G‑GGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGAT                                                 >  SRR4302542.804651/1‑94 (MQ=60)
                                          ACGACGCCGAAATGCTGTCGATGGC‑‑G‑GGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGAT                                                 <  SRR4302542.5819593/94‑1 (MQ=60)
                                           CGACGCCGAAATGCTGTCGATGGC‑‑G‑GGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATT                                                >  SRR4302542.93518/1‑94 (MQ=60)
                                           CGACGCCGAAATGCTGTCGATGGC‑‑G‑GGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATT                                                <  SRR4302542.5246568/94‑1 (MQ=60)
                                           CGACGCCGAAATGCTGTCGATGGC‑‑G‑GGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATT                                                <  SRR4302542.5366810/94‑1 (MQ=60)
                                           CGACGCCGAAATGCTGTCGATGGC‑‑G‑GGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATT                                                >  SRR4302542.7206434/1‑94 (MQ=60)
                                             ACGCCGAAATGCTGTCGATGGC‑‑G‑GGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGG                                              >  SRR4302542.6682712/1‑94 (MQ=60)
                                             ACGCCGAAATGCTGTCGATGGC‑‑G‑GGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGG                                              <  SRR4302542.6057594/94‑1 (MQ=60)
                                              CGCCGAAATGCTGTCGATTGC‑‑GTGG‑‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGT                                             <  SRR4302542.941628/94‑1 (MQ=60)
                                              CGCCGAAATGCTGTCGATGGC‑‑G‑GGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGT                                             <  SRR4302542.4214869/94‑1 (MQ=60)
                                              CGCCGAAATGCTGTCGATGGC‑‑G‑GGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGT                                             >  SRR4302542.1479849/1‑94 (MQ=60)
                                              CGCCGAAATGCTGTCGATGGC‑‑G‑GGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGT                                             >  SRR4302542.3956601/1‑94 (MQ=60)
                                               GCCGAAATGCTGTCGATGGC‑‑G‑GGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTA                                            >  SRR4302542.7442644/1‑94 (MQ=60)
                                               GCCGAAATGCTGTCGATGGC‑‑G‑GGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTA                                            <  SRR4302542.726156/94‑1 (MQ=60)
                                               GCCGAAATGCTGTCGATGGC‑‑G‑GGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTA                                            >  SRR4302542.683073/1‑94 (MQ=60)
                                                CCGAAATGCTGTCGATGGC‑‑G‑GGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTAC                                           >  SRR4302542.5699024/1‑94 (MQ=60)
                                                CCGAAATGCTGTCGATGGC‑‑G‑GGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTAC                                           <  SRR4302542.2775570/94‑1 (MQ=60)
                                                 CGAAATGCTGTCGATTGC‑‑G‑GGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACT                                          >  SRR4302542.4516623/1‑94 (MQ=60)
                                                 CGAAATGCTGTCGATGGC‑‑G‑GGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACT                                          <  SRR4302542.3981070/94‑1 (MQ=60)
                                                  GAGATGCTGTCGATGGC‑‑G‑GGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTA                                         <  SRR4302542.5917868/94‑1 (MQ=60)
                                                  GAAATGCTGTCGATGGC‑‑G‑GGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTA                                         <  SRR4302542.5501553/94‑1 (MQ=60)
                                                  GAAATGCTGTCGATGGC‑‑G‑GGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTA                                         >  SRR4302542.1326904/1‑94 (MQ=60)
                                                  GAAATGCTGTCGATGGC‑‑G‑GGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTA                                         >  SRR4302542.4803959/1‑94 (MQ=60)
                                                   AAATGCTGTCGATGGC‑‑G‑GGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAA                                        <  SRR4302542.2459952/94‑1 (MQ=60)
                                                    AATGCTGTCGATGGC‑‑G‑GGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAAT                                       <  SRR4302542.975088/94‑1 (MQ=60)
                                                    AATGCTGTCGATGGC‑‑G‑GGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAAT                                       >  SRR4302542.3535764/1‑94 (MQ=60)
                                                    AATGCTGTCGATGGC‑‑G‑GGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAAT                                       >  SRR4302542.2450408/1‑94 (MQ=60)
                                                     ATGCTGTCGATGGC‑‑G‑GGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATG                                      >  SRR4302542.1665629/1‑94 (MQ=60)
                                                      TGCTGTCGATGGC‑‑G‑GGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGC                                     <  SRR4302542.6534032/94‑1 (MQ=60)
                                                      TGCTGTCGATGGC‑‑G‑GGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGC                                     <  SRR4302542.850918/94‑1 (MQ=60)
                                                       GCTGTCGATGGC‑‑G‑GGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGGGCTGGAAGTGATTGGTACTAATGCC                                    <  SRR4302542.664908/94‑1 (MQ=60)
                                                       GCTGTCGATGGC‑‑G‑GGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCC                                    <  SRR4302542.4449290/94‑1 (MQ=60)
                                                       GCTGTCGATGGC‑‑G‑GGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCC                                    >  SRR4302542.7067747/1‑94 (MQ=60)
                                                       GCTGTCGATGGC‑‑G‑GGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCC                                    >  SRR4302542.942954/1‑94 (MQ=60)
                                                       GCTGTCGATGGC‑‑G‑GGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCC                                    <  SRR4302542.522300/94‑1 (MQ=60)
                                                        CTGTCGATGGC‑‑G‑GGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGGGCTGGAAGTGATTGGTACTAATGCCG                                   >  SRR4302542.4280022/1‑94 (MQ=60)
                                                          GTCGATGGC‑‑G‑GGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGGGCTGGAAGTGATTGGTACTAATGCCGAC                                 <  SRR4302542.561757/94‑1 (MQ=60)
                                                          GTCGATGGC‑‑G‑GGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGAC                                 <  SRR4302542.1627799/94‑1 (MQ=60)
                                                          GTCGATGGC‑‑G‑GGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGAC                                 <  SRR4302542.3149290/94‑1 (MQ=60)
                                                          GTCGATGGC‑‑G‑GGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGAC                                 <  SRR4302542.4028989/94‑1 (MQ=60)
                                                          GTCGATGGC‑‑G‑GGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGAC                                 <  SRR4302542.3815007/94‑1 (MQ=60)
                                                          GTCGATGGC‑‑G‑GGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGAC                                 <  SRR4302542.2259085/94‑1 (MQ=60)
                                                          GTCGATGGC‑‑G‑GGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGAC                                 <  SRR4302542.3660641/94‑1 (MQ=60)
                                                          GTCGATGGC‑‑G‑GGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGAC                                 <  SRR4302542.210861/94‑1 (MQ=60)
                                                          GTCGATGGC‑‑G‑GGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGAC                                 <  SRR4302542.1706888/94‑1 (MQ=60)
                                                           TCGATGGC‑‑G‑GGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACG                                <  SRR4302542.3636446/94‑1 (MQ=60)
                                                           TCGATGGC‑‑G‑GGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACG                                <  SRR4302542.76017/94‑1 (MQ=60)
                                                             GATGGC‑‑G‑GGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGAC                              <  SRR4302542.1700591/94‑1 (MQ=60)
                                                             GATGGC‑‑G‑GGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGAC                              >  SRR4302542.5555519/1‑94 (MQ=60)
                                                              ATGGCGGG‑GG‑‑AAG‑GCTGCATTATGGGGAGTGCGCACCAGCGGCTGAAAGACCTTCATCCCGAGCTGGGAGGGATTGGTACTAATGCCGACGACG                             >  SRR4302542.4496968/1‑94 (MQ=42)
                                                               TGGC‑‑G‑GGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGC                            <  SRR4302542.7456029/94‑1 (MQ=60)
                                                                GGC‑‑G‑GGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCG                           <  SRR4302542.6116619/94‑1 (MQ=60)
                                                                GGC‑‑G‑GGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCG                           <  SRR4302542.5686466/94‑1 (MQ=60)
                                                                     gcggggaaagGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGG                          <  SRR4302542.5806576/84‑1 (MQ=60)
                                                                     G‑GG‑‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGgtg                       >  SRR4302542.2440564/1‑91 (MQ=60)
                                                                      cggggaaagGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGT                         >  SRR4302542.1565145/10‑94 (MQ=60)
                                                                      cggggaaagGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGT                         <  SRR4302542.1735699/85‑1 (MQ=60)
                                                                      cggggaaagGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGT                         <  SRR4302542.4943817/85‑1 (MQ=60)
                                                                       ggggaaagGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGGGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTG                        <  SRR4302542.3564753/86‑1 (MQ=60)
                                                                       ggggaaagGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTG                        <  SRR4302542.7285909/86‑1 (MQ=60)
                                                                       ggggaaagGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTG                        <  SRR4302542.1365604/86‑1 (MQ=60)
                                                                       ggggaaagGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTG                        <  SRR4302542.2681249/86‑1 (MQ=60)
                                                                       ggggaaagGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTG                        <  SRR4302542.761829/86‑1 (MQ=60)
                                                                       GG‑‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCC                      <  SRR4302542.442763/94‑1 (MQ=60)
                                                                       GG‑‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCC                      >  SRR4302542.5318198/1‑94 (MQ=60)
                                                                        G‑‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCG                     <  SRR4302542.127578/94‑1 (MQ=60)
                                                                        G‑‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCG                     <  SRR4302542.388595/94‑1 (MQ=60)
                                                                           AAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGGGATTGGGACTAATGCCGACGACGCGGGGCCGCA                   >  SRR4302542.6213465/1‑94 (MQ=60)
                                                                           AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGC                    >  SRR4302542.3796664/1‑94 (MQ=60)
                                                                           AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGC                    >  SRR4302542.1700078/1‑94 (MQ=60)
                                                                           AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGC                    <  SRR4302542.3345725/94‑1 (MQ=60)
                                                                            AG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCAT                  >  SRR4302542.162357/1‑94 (MQ=60)
                                                                            AG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCAT                  <  SRR4302542.5228594/94‑1 (MQ=60)
                                                                             G‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATT                 <  SRR4302542.3946125/94‑1 (MQ=60)
                                                                             G‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATT                 >  SRR4302542.3934760/1‑94 (MQ=60)
                                                                               GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTA                >  SRR4302542.2129670/1‑94 (MQ=60)
                                                                               GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTA                >  SRR4302542.2967139/1‑94 (MQ=60)
                                                                                CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGGGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTAT               <  SRR4302542.910572/94‑1 (MQ=60)
                                                                                CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTAT               <  SRR4302542.5348106/94‑1 (MQ=60)
                                                                                 TGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGGGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATC              <  SRR4302542.6309641/94‑1 (MQ=60)
                                                                                 TGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATC              <  SRR4302542.5288428/94‑1 (MQ=60)
                                                                                 TGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATC              <  SRR4302542.6688071/94‑1 (MQ=60)
                                                                                 TGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATC              <  SRR4302542.431090/94‑1 (MQ=60)
                                                                                 TGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATC              >  SRR4302542.1830330/1‑94 (MQ=60)
                                                                                 TGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATC              >  SRR4302542.2937832/1‑94 (MQ=60)
                                                                                  GCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGGGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCT             <  SRR4302542.1807889/94‑1 (MQ=60)
                                                                                  GCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCT             >  SRR4302542.5175594/1‑94 (MQ=60)
                                                                                  GCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCT             <  SRR4302542.4716048/94‑1 (MQ=60)
                                                                                  GCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCT             <  SRR4302542.5866035/94‑1 (MQ=60)
                                                                                  GCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCT             >  SRR4302542.6817928/1‑94 (MQ=60)
                                                                                   CATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGGGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTG            >  SRR4302542.6214823/1‑94 (MQ=60)
                                                                                   CATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTG            >  SRR4302542.2208721/1‑94 (MQ=60)
                                                                                   CATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTG            <  SRR4302542.7149721/94‑1 (MQ=60)
                                                                                   CATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTG            <  SRR4302542.939621/94‑1 (MQ=60)
                                                                                   CATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTG            <  SRR4302542.3322838/94‑1 (MQ=60)
                                                                                    ATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGGGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGC           >  SRR4302542.2177329/1‑94 (MQ=60)
                                                                                    ATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGC           <  SRR4302542.1169463/94‑1 (MQ=60)
                                                                                    ATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGC           >  SRR4302542.4779470/1‑94 (MQ=60)
                                                                                    ATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGC           >  SRR4302542.5482600/1‑94 (MQ=60)
                                                                                    ATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGC           >  SRR4302542.3461139/1‑94 (MQ=60)
                                                                                    ATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGC           >  SRR4302542.3279601/1‑94 (MQ=60)
                                                                                     TTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCG          <  SRR4302542.454162/94‑1 (MQ=60)
                                                                                     TTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCG          <  SRR4302542.3424448/94‑1 (MQ=60)
                                                                                      TATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGT         <  SRR4302542.5828866/94‑1 (MQ=60)
                                                                                      TATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGT         <  SRR4302542.4408040/94‑1 (MQ=60)
                                                                                      TATGGGCAGTGCGCACCAGCGCCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGT         >  SRR4302542.3190880/1‑94 (MQ=60)
                                                                                       ATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTA        >  SRR4302542.5495244/1‑94 (MQ=60)
                                                                                        TGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAA       >  SRR4302542.1812266/1‑94 (MQ=60)
                                                                                        TGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAA       >  SRR4302542.4265218/1‑94 (MQ=60)
                                                                                         GGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAA      <  SRR4302542.6845379/94‑1 (MQ=60)
                                                                                         GGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAA      >  SRR4302542.3554056/1‑94 (MQ=60)
                                                                                          GGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAAC     <  SRR4302542.2603331/94‑1 (MQ=60)
                                                                                          GGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCATAAAC     <  SRR4302542.6567999/94‑1 (MQ=60)
                                                                                           GCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACT    <  SRR4302542.1587894/94‑1 (MQ=60)
                                                                                           GCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACT    >  SRR4302542.5738241/1‑94 (MQ=60)
                                                                                           GCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACT    <  SRR4302542.5687256/94‑1 (MQ=60)
                                                                                           GCAGTGCGCACCAGCGGCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACT    <  SRR4302542.4788453/94‑1 (MQ=60)
                                                                                            CAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTC   >  SRR4302542.4398541/1‑94 (MQ=60)
                                                                                            CAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTC   >  SRR4302542.7178122/1‑94 (MQ=60)
                                                                                            CAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTC   <  SRR4302542.1421127/94‑1 (MQ=60)
                                                                                            CAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTC   >  SRR4302542.6576707/1‑94 (MQ=60)
                                                                                             AGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGGGCTGGAAGTGATTGGTATTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCT  <  SRR4302542.2358534/94‑1 (MQ=60)
                                                                                             AGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCT  <  SRR4302542.1588782/94‑1 (MQ=60)
                                                                                                 |                                                                                         
GCTACAG‑CTGAAGGATTG‑ATTGCGTT‑‑GTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑G‑GG‑‑AAA‑GCTGCATTATGGGCAGTGGCCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑GAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCT  >  NC_000913/4008404‑4008579

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑