Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1061 294.1 15065038 98.6% 14854127 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,080,831 G→A L858L (CTG→TTG)  fdoG ← formate dehydrogenase‑O, major subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,080,8310GA85.0% 706.7 / 84.5 268L858L (CTG→TTG) fdoGformate dehydrogenase‑O, major subunit
Reads supporting (aligned to +/- strand):  ref base G (24/16);  new base A (115/111);  total (140/128)
Fisher's exact test for biased strand distribution p-value = 3.08e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.06e-01

Only 100 of 277 total aligned reads displayed.

TTATCGGCTTTACCCAATGCTTCGGCGTCGTCTTTAAAGATACGTGCAGCCGGGTTCGAGATAACGTTTGGATGCAGCGGGTTAGTTCCCAGCGGCGTTTCAAACGGCTCGTAGTGTTCCGGGAACGGACCTTCCGCCATCTTATCGAGGGCAAACAGACGCCCCATGCCTTCCTGCTGCAT  >  NC_000913/4080740‑4080921
                                                                                           |                                                                                          
 tATCGGCTTTACCCAATGCTTCGGCGTCGTCTTTAAAGATACGTGCAGCCGGGTTCGAGATAACGTTTGGATGCAGCGGGTTAGTTCCCAacg                                                                                          >  1:5024173/1‑93 (MQ=255)
 tATCGGCTTTACCCAATGCTTCGGCGTCGTCTTTAAAGATACGTGCAGCCGGGTTCGAGATAACGTTTGGATGCAGCGGGTTAGTTCCCAacg                                                                                          <  1:5341549/93‑1 (MQ=255)
   tCGGCTTTACCCAATGCTTCGGCGTCGTCTTTAAAGATACGTGCAGCCGGGTTCGAGATAACGTTTGGATGCAGCGGGTTAGTTCCCAacggc                                                                                        <  1:4577226/93‑1 (MQ=255)
      gCTTTACCCAATGCTTCGGCGTCGTCTTTAAAGATATGTGCAGCCGGGTTCGAGATAACGTTTGGATGCAGCGGGTTAGTTCCCAACGGCGtt                                                                                     <  1:4708845/93‑1 (MQ=255)
      gCTTTACCCAATGCTTCGGCGTCGTCTTTAAAGATACGTGCAGCCGGGTTCGAGATAACGTTTGGATGCAGCGGGTTAGTTCCCAACGGCGtt                                                                                     >  2:6314382/1‑93 (MQ=255)
        tttACCCAATGCTTCGGCGTCGTCTTTAAAGATACGTGCAGCCGGGTTCGAGATAACGTTTGGATGCAGCGGGTTAGTTCCCAGCGGCGTTTc                                                                                   >  2:5163034/1‑93 (MQ=255)
        tttACCCAATGCTTCGGCGTCGTCTTTAAAGATACGTGCAGCCGGGTTCGAGATAACGTTTGGATGCAGCGGGTTAGTTCCCAACGGCGTTTc                                                                                   <  1:1429008/93‑1 (MQ=255)
        tttACCCAATGCTTCGGCGTCGTCTTTAAAGATACGTGCAGCCGGGTTCGAGATAACGTTTGGATGCAGCGGGTTAGTTCCCAACGGCGTTTc                                                                                   >  2:470517/1‑93 (MQ=255)
        tttACCCAATGCTTCGGCGTCGTCTTTAAAGATACGTGCAGCCGGGTTCGAGATAACGTTTGGATGCAGCGGGTTAGTTCCCAACGGCGTTTc                                                                                   >  2:6876358/1‑93 (MQ=255)
          tACCCAATGCTTCGGCGTCGTCTTTAAAGATACGTGCAGCCGGGTTCGAGATAACGTTTGGATGCAGCGGGTTAGTTCCCAACGGCGTTTCaa                                                                                 <  1:6201696/93‑1 (MQ=255)
           aCCCAATGCTTCGGCGTCGTCTTTAAAGATACGTGCAGCCGGGTTCGAGATAACGTTTGGATGCAGCGGGTTAGTTCCCAACGGCGTTTCaaa                                                                                <  1:6829354/93‑1 (MQ=255)
           aCCCAATGCTTCGGCGTCGTCTTTAAAGATACGTGCAGCCGGGTTCGAGATAACGTTTGGATGCAGCGGGTTAGTTCCCAACGGCGTTTCaaa                                                                                <  1:6016319/93‑1 (MQ=255)
            cccAATGCTTCGGCGTCGTCTTTAAAGATACGTGCAGCCGGGTTCGAGATAACGTTTGGATGCAGCGGGTTAGTTCCCAGCGGCGTTTCAAAc                                                                               >  1:7009002/1‑93 (MQ=255)
             ccAATGCTTCGGCGTCGTCTTTAAAGATACGTGCAGCCGGGTTCGAGATAACGTTTGGATGCAGCGGGTTAGTTCCCAACGGCGTTTCAAACg                                                                              >  2:5360809/1‑93 (MQ=255)
               aaTGCTTCGGCGTCGTCTTTAAAGATACGTGCAGCCGGGTTCGAGATAACGTTTGGATGCAGCGGGTTAGTTCCCAGCGGCGTTTCAAACGGc                                                                            <  1:4427970/93‑1 (MQ=255)
               aaTGCTTCGGCGTCGTCTTTAAAGATACGTGCAGCCGGGTTCGAGATAACGTTTGGATGCAGCGGGTTAGTTCCCAACGGCGTTTCAAACGGc                                                                            >  2:2719053/1‑93 (MQ=255)
                aTGCTTCGGCGTCGTCTTTAAAGATACGTGCAGCCGGGTTCGAGATAACGTTTGGATGCAGCGGGTTAGTTCCCAACGGCGTTTCAAACGGCt                                                                           >  2:7326518/1‑93 (MQ=255)
                 tGCTTCGGCGTCGTCTTTAAAGATACGTGCAGCCGGGTTCGAGATAACGTTTGGATGCAGCGGGTTAGTTCCCAACGGCGTTTCAAACGGCTc                                                                          <  1:3620897/93‑1 (MQ=255)
                 tGCTTCGGCGTCGTCTTTAAAGATACGTGCAGCCGGGTTCGAGATAACGTTTGGATGCAGCGGGTTAGTTCCCAACGGCGTTTCAAACGGCTc                                                                          <  2:5712882/93‑1 (MQ=255)
                     tCGGCGTCGTCTTTAAAGATACGTGCAGCCGGGTTCGAGATAACGTTTGGATGCAGCGGGTTAGTTCCCAGCGGCGTTTCAAACGGCTCGTAg                                                                      <  1:597084/93‑1 (MQ=255)
                       ggCGTCGTCTTTAAAGATACGTGCAGCCGGGTTCGAGATAACGTTTGGATGCAGCGGGTTAGTTCCCAGCGGCGTTTCAAACGGCTCGtcggg                                                                    >  1:6920333/1‑89 (MQ=255)
                        gCGTCGTCTTTAAAGATACGTGCAGCCGGGTTCGAGATAACGTTTGGATGCAGCGGGTTAGTTCCCAACGGCGTTTCAAACGGCTCGGAgggt                                                                   >  1:1036466/1‑93 (MQ=255)
                        gCGACGTCTTTAAAGATACGTGCAGCCGGGTTCGAGATAACGTTTGGATGCAGCGGGTTAGTTCCCAACGGCGTTTCAAACGGCTCGTAgtgt                                                                   <  2:5975263/93‑1 (MQ=255)
                          gtcgtcTTTAAAGATACGTGCAGCCGGGTTCGAGATAACGTTTGGATGCAGCGGGTTAGTTCCCAACGGCGTTTCAAACGGCTCGTAGTGTTc                                                                 <  2:7059703/93‑1 (MQ=255)
                          gtcgtcTTTAAAGATACGTGCAGCCGGGTTCGAGATAACGTTTGGATGCAGCGGGTTAGTTCCCAACGGCGTTTCAAACGGCTCGTAGTGTTc                                                                 >  1:5250255/1‑93 (MQ=255)
                          gtcgtcTTTAAAGATACGTGCAGCCGGGTTCGAGATAACGTTTGGATGCAGCGGGTTAGTTCCCAACGGCGTTTCAAACGGCTCGTAGTGTTc                                                                 <  1:3642717/93‑1 (MQ=255)
                           tcgtcTTTAAAGATACGTGCAGCCGGGTTCGAGATAACGTTTGGATGCAGCGGGTTAGTTCCCAACGGCGTTTCAAACGGCTCGTAGTGTTcc                                                                >  2:2984950/1‑93 (MQ=255)
                           tcgtcTTTAAAGATACGTGCAGCCGGGTTCGAGATAACGTTTGGATGCAGCGGGTTAGTTCCCAACGGCGTTTCAAACGGCTCGTAGTGTTcc                                                                >  2:6068814/1‑93 (MQ=255)
                           tcgtcTTTAAAGATACGTGCAGCCGGGTTCGAGATAACGTTTGGATGCAGCGGGTTAGTTCCCAACGGCGTTTCAAACGGCTCGTAGTGTTcc                                                                >  1:1740463/1‑93 (MQ=255)
                           gcgtcTTTAAAGATACGTGCAGCCGGGTTCGAGATAACGTTTGGATGCAGCGGGTTAGTTCCCAACGGCGTTTCAAACGGCTCGTAGTGTTcc                                                                <  1:960493/92‑1 (MQ=255)
                            cgtcTTTAAAGATACGTGCAGCCGGGTTCGAGATAACGTTTGGATGCAGCGGGTTAGTTCCCAACGGCGTTTCAAACGGCTCGTAGTGTTCCg                                                               >  2:2372567/1‑93 (MQ=255)
                             gtcTTTAAAGATACGTGCAGCCGGGTTCGAGATAACGTTTGGATGCAGCGGGTTAGTTCCCAACGGCGTTTCAAACGGCTCGTAGTGTTcccg                                                              >  2:3652953/1‑91 (MQ=255)
                              tcTTTAAAGATACGTGCAGCCGGGTTCGAGATAACGTTTGGATGCAGCGGGTTAGTTCCCACCGCCGTTTAAAACGGCTCGAATGGTTCCggg                                                             >  2:4737685/1‑93 (MQ=37)
                              tcTTTAAAGATACGTGCAGCCGGGTTCGAGATAACGTTTGGATGCAGCGGGTTAGTTCCCAACGGCGTTTCAAACGGCTCGTAGTGTTCCggg                                                             >  2:5074737/1‑93 (MQ=255)
                                tttAAAGATACGTGCAGCCGGGTTCGAGATAACGTTTGGATGCAGCGGGTTAGTTCCCAACGGCGTTTCAAACGGCTCGTAGTGTTCCGGGaa                                                           >  2:1255873/1‑93 (MQ=255)
                                 ttAAAGATACGTGCAGCCGGGTTCGAGATAACGTTTGGATGCAGCGGGTTAGTTCCCAACGGCGTTTCAAACGGCTCGTAGTGTTCCGGGAAc                                                          <  1:3119919/93‑1 (MQ=255)
                                  tAAAGATACGTGCAGCCGGGTTCGAGATAACGTTTGGATGCAGCGGGTTAGTTCCCAACGGCGTTTCAAACGGCTCGTAGTGTTCCGGGAACg                                                         >  2:2307356/1‑93 (MQ=255)
                                   aaaGATACGTGCAGCCGGGTTCGAGATAACGTTTGGATGCAGCGGGTTAGTTCCCAACGGCGTTTCAAACGGCTCGTAGTGTTCCGGGAACgg                                                        >  2:1982703/1‑93 (MQ=255)
                                    aaGATACGTGCAGCCGGGTTCGAGATAACGTTTGGATGCAGCGGGTTAGTTCCCAACGGCGTTTCAAACGGCTCGTAGTGTTCCGGGAACGGa                                                       <  2:2752201/93‑1 (MQ=255)
                                     aGATACGTGCAGCCGGGTTCGAGATAACGTTTGGATGCAGCGGGTTAGTTCCCAGCGGCGTTTCAAACGGCTCGTAGTGTTCCGGGAACGGAc                                                      >  1:5087636/1‑93 (MQ=255)
                                     aGATACGTGCAGCCGGGTTCGAGATAACGTTTGGATGCAGCGGGTTAGTTCCCAACGGCGTTTCAAACGGCTCGTAGTGTTCCGGGAACGGAc                                                      >  2:3203973/1‑93 (MQ=255)
                                       aTACGTGCAGCCGGGTTCGAGATAACGTTTGGATGCAGCGGGTTAGTTCCCAACGGCGTTTCAAACGGCTCGTAGTGTTCCGGGAACGGACCt                                                    <  2:178139/93‑1 (MQ=255)
                                       aTACGTGCAGCCGGGTTCGAGATAACGTTTGGATGCAGCGGGTTAGTTCCCAACGGCGTTTCAAACGGCTCGTAGTGTTCCGGGAACGGACCt                                                    <  1:2257697/93‑1 (MQ=255)
                                        tACGTGCAGCCGGGTTCGAGATAACGTTTGGATGCAGCGGGTTAGTTCCCAACGGCGTTTCAAACGGCTCGTAGTGTTCCGGGAACGGACCtt                                                   >  1:5396108/1‑93 (MQ=255)
                                         aCGTGCAGCCGGGTTCGAGATAACGTTTGGATGCAGCGGGTTAGTTCCCAACGGCGTTTCAAACGGCTCGTAGTGTTCCGGGAACGGACCTTc                                                  <  1:2909847/93‑1 (MQ=255)
                                            tGCAGCCGGGTTCGAGATAACGTTTGGATGCAGCGGGTTAGTTCCCAACGGCGTTTCAAACGGCTCGTAGTGTTCCGGGAACGGACCTTCCGc                                               >  2:438511/1‑93 (MQ=255)
                                              cAGCCGGGTTCGAGATAACGTTTGGATGCAGCGGGTTAGTTCCCAGCGGCGTTTCAAACGGCTCGTAGTGTTCCGGGAACGGACCTTCCGCCa                                             >  1:4232843/1‑93 (MQ=255)
                                               aGCCGGGTTCGAGATAACGTTTGGATGCAGCGGGTTAGTTCCCAACGGCGTTTCAAACGGCTCGTAGTGTTCCGGGAACGGACCTTCCGCCAt                                            >  2:5591555/1‑93 (MQ=255)
                                                gCCGGGTTCGAGATAACGTTTGGATGCAGCGGGTTAGTTCCCAACGGCGTTTCAAACGGCTCGTAGTGTTCCGGGAACGGACCTTCCGCCATc                                           <  1:3464403/93‑1 (MQ=255)
                                                gCCGGGTTCGAGATAACGTTTGGATGCAGCGGGTTAGTTCCCAACGGCGTTTCAAACGGCTCGTAGTGTTCCGGGAACGGACCTTCCGCCATc                                           >  2:2068630/1‑93 (MQ=255)
                                                  cGGGTTCGAGATAACGTTTGGATGCAGCGGGTTAGTTCCCAACGGCGTTTCAAACGGCTCGTAGTGTTCCGGGAACGGACCTTCCGCCATCtt                                         >  2:3300577/1‑93 (MQ=255)
                                                   gggTTCGAGATAACGTTTGGATGCAGCGGGTTAGTTCCCAACGGCGTTTCAAACGGCTCGTAGTGTTCCGGGAACGGACCTTCCGCCATCTTa                                        <  1:372775/93‑1 (MQ=255)
                                                    ggTTCGAGATAACGTTTGGATGCAGCGGGTTAGTTCCCAACGGCGTTTCAAACGGCTCGTAGTGTTCCGGGGACGGACCTTCCGCCATCTTAt                                       >  2:1397665/1‑93 (MQ=255)
                                                    ggTTCGAGATAACGTTTGGATGCAGCGGGTTAGTTCCCAACGGCGTTTCAAACGGCTCGTAGTGTTCCGGGAACGGACCTTCCGCCATCTTAt                                       >  2:5415242/1‑93 (MQ=255)
                                                    ggTTCGAGATAACGTTTGGATGCAGCGGGTTAGTTCCCAACGGCGTTTCAAACGGCTCGTAGTGTTCCGGGAACGGACCTTCCGCCATCTTAt                                       >  1:5935709/1‑93 (MQ=255)
                                                     gTTCGAGATAACGTTTGGATGCAGCGGGTTAGTTCCCAACGGCGTTTCAAACGGCTCGTAGTGTTCCGGGAACGGACCTTCCGCCCTCTTATc                                      >  1:3197040/1‑93 (MQ=255)
                                                     gTTCGAGATAACGTTTGGATGCAGCGGGTTAGTTCCCAACGGCGTTTCAAACGGCTCGTAGTGTTCCGGGAACGGACCTTCCGCCATCTTATc                                      <  1:613178/93‑1 (MQ=255)
                                                      ttCGAGATAACGTTTGGATGCAGCGGGTTAGTTCCCAGCGGCGTTTCAAACGGCTCGTAGTGTTCCGGGAACGGACCTTCCGCCATCTTATCg                                     >  1:4593499/1‑93 (MQ=255)
                                                       tCGAGATAACGTTTGGATGCAGCGGGTTAGTTCCCAACGGCGTTTCAAACGGCTCGTAGTGTTCCGGGAACGGACCTTCCGCCATCTTATCGa                                    <  2:1911338/93‑1 (MQ=255)
                                                       tCGAGATAACGTTTGGATGCAGCGGGTTAGTTCCCAACGGCGTTTCAAACGGCTCGTAGTGTTCCGGGAACGGACCTTCCGCCATCTTATCGa                                    <  1:1493752/93‑1 (MQ=255)
                                                       tCGAGATAACGTTTGGATGCAGCGGGTTAGTTCCCAACGGCGTTTCAAACGGCTCGTAGTGTTCCGGGAACGGACCTTCCGCCATCTTATCGa                                    <  2:4889999/93‑1 (MQ=255)
                                                        cGAGATAACGTTTGGATGCAGCGGGTTAGTTCCCAACGGCGTTTCAAACGGCTCGTAGTGTTCCGGGAACGGACCTTCCGCCATCTTATCGAg                                   >  2:803885/1‑93 (MQ=255)
                                                         gagaTAACGTTTGGATGCAGCGGGTTAGTTCCCAACGGCGTTTCAAACGGCTCGTAGTGTTCCGGGAACGGACCTTCCGCCATCTTATCGAgg                                  <  1:1187718/93‑1 (MQ=255)
                                                         gagaTAACGTTTGGATGCAGCGGGTTAGTTCCCAACGGCGTTTCAAACGGCTCGTAGTGTTCCGGGAACGGACCTTCCGCCATCTTATCGAgg                                  >  1:5138063/1‑93 (MQ=255)
                                                           gaTAACGTTTGGATGCAGCGGGTTAGTTCCCAGCGGCGTTTCAAACGGCTCGTAGTGTTCCGGGAACGGACCTTCCGCCATCTTATCGAGGGc                                >  2:5896257/1‑93 (MQ=255)
                                                           gaTAACGTTTGGATGCAGCGGGTTAGTTCCCAACGGCGTTTCAAACGGCTCGTAGTGTTCCGGGAACGGACCTTCCGCCATCTTATCGAggga                                >  2:3889593/1‑92 (MQ=255)
                                                           gaTAACGTTTGGATGCAGCGGGTTAGTTCCCAACGGCGTTTCAAACGGCTCGTAGTGTTCCGGGAACGGACCTTCCGCCATCTTATCGAGGGc                                >  1:1889270/1‑93 (MQ=255)
                                                           gaTAACGTTTGGATGCAGCGGGTTAGTTCCCAACGGCGTTTCAAACGGCTCGTAGTGTTCCGGGAACGGACCTTCCGCCATCTTATCGAGGGc                                >  2:6632940/1‑93 (MQ=255)
                                                            aTAACGTTTGGATGCAGCGGGTTAGTTCCCAACGGCGTTTCAAACGGCTCGTAGTGTTCCGGGAACGGACCTTCCGCCATCTTATCGAGGGCa                               >  2:1726152/1‑93 (MQ=255)
                                                              aaCGTTTGGATGCAGCGGGTTAGTTCCCAACGGCGTTTCAAACGGCTCGTAGTGTTCCGGGAACGGACCTTCCGCCATCTTATCGAGGGCaaa                             >  1:1537279/1‑93 (MQ=255)
                                                               aCGTTTGGATGCAGCGGGTTAGTTCCCAACGGCGTTTCAAACGGCTCGTAGTGTTCCGGGAACGGACCTTCCGCCATCTTATCGAGGGCAAAc                            <  2:4116199/93‑1 (MQ=255)
                                                                cGTTTGGATGCAGCGGGTTAGTTCCCAGCGGCGTTTCAAACGGCTCGTAGTGTTCCGGGAACGGACCTTCCGCCATCTTATCGAGGGCAAACa                           <  2:3437832/93‑1 (MQ=255)
                                                                  tttGGATGCAGCGGGTTAGTTCCCAACGGCGTTTCAAACGGCTCGTAGTGTTCCGGGAACGGACCTTCCGCCATCTTATCGAGGGCAAACAGa                         <  2:2405402/93‑1 (MQ=255)
                                                                  tttGGATGCAGCGGGTTAGTTCCCAACGGCGTTTCAAACGGCTCGTAGTGTTCCGGGAACGGACCTTCCGCCATCTTATCGAGGGCAAACAGa                         <  2:6742064/93‑1 (MQ=255)
                                                                    tGGATGCAGCGGGTTAGTTCCCAACGGCGTTTCAAACGGCTCGTAGTGTTCCGGGAACGGACCTTCCGCCATCTTATCGAGGGCAAACAGACg                       >  1:2619830/1‑93 (MQ=255)
                                                                        tGCAGCGGGTTAGTTCCCAACGGCGTTTCAAACGGCTCGTAGTGTTCCGGGAACGGACCTTCCGCCATCTTATCGAGGGCAAACAGACGcccc                   >  1:5677160/1‑93 (MQ=255)
                                                                        tGCAGCGGGTTAGTTCCCAACGGCGTTTCAAACGGCTCGTAGTGTTCCGGGAACGGACCTTCCGCCATCTTATCGAGGGCAAACAGACGcccc                   >  2:6589024/1‑93 (MQ=255)
                                                                         gCAGCGGGTTAGTTCCCAGCGGCGTTTCAAACGGCTCGTAGTGTTCCGGGAACGGACCTTCCGCCATCTTATCGAGGGCAAACAGACGCCCCa                  <  1:4913011/93‑1 (MQ=255)
                                                                          cAGCGGGTTAGTTCCCAACGGCGTTTCAAACGGCTCGTAGTGTTCCGGGAACGGACCTTCCGCCATCTTATCGAGGGCAAACAGACGCCCCAt                 <  2:358515/93‑1 (MQ=255)
                                                                           aGCGGGTTAGTTCCCAACGGCGTTTCAAACGGCTCGTAGTGTTCCGGGAACGGACCTTCCGCCATCTTATCGAGGGCAAACAGACGCCCCATg                <  1:7267369/93‑1 (MQ=255)
                                                                             cGGGTTAGTTCCCAGCGGCGTTTCAAACGGCTCGTAGTGTTCCGGGAACGGACCTTCCGCCATCTTATCGAGGGCAAACAGACGCCCCATGcc              >  2:31149/1‑93 (MQ=255)
                                                                             cGGGTTAGTTCCCAACGGCGTTTCAAACGGCTCGTAGTGTTCCGGGAACGGACCTTCCGCCATCTTATCGAGGGCAAACAGACGCCCCATGcc              <  1:4373196/93‑1 (MQ=255)
                                                                             cGGGTTAGTTCCCAACGGCGTTTCAAACGGCTCGTAGTGTTCCGGGAACGGACCTTCCGCCATCTTATCGAGGGCAAACAGACGCCCCATGcc              <  1:2528282/93‑1 (MQ=255)
                                                                             cGGGTTAGTTCCCAACGGCGTTTCAAACGGCTCGTAGTGTTCCGGGAACGGACCTTCCGCCATCTTATCGAGGGCAAACAGACGCCCCATGcc              <  2:2166665/93‑1 (MQ=255)
                                                                              gggTTAGTTCCCAACGGCGTTTCAAACGGCTCGTAGTGTTCCGGGAACGGACCTTCCGCCATCTTATCGAGGGCAAACAGACGCCCCATGCCt             >  2:2474252/1‑93 (MQ=255)
                                                                               ggTTAGTTCCCAGCGGCGTTTCAAACGGCTCGTAGTGTTCCGGGAACGGACCTTCCGCCATCTTATCGAGGGCAAACAGACGCCCCATGCCtt            <  2:6982039/93‑1 (MQ=255)
                                                                               ggTTAGTTCCCAGCGGCGTTTCAAACGGCTCGTAGTGTTCCGGGAACGGACCTTCCGCCATCTTATCGAGGGCAAACAGACGCCCCATGCCtt            >  2:6175414/1‑93 (MQ=255)
                                                                               ggTTAGTTCCCAACGGCGTTTCAAACGGCTCGTAGTGTTCCGGGAACGGACCTTCCGCCATCTTATCGAGGGCAAACAGACGCCCCATGCCtt            <  2:1314036/93‑1 (MQ=255)
                                                                               ggTTAGTTCCCAACGGCGTTTCAAACGGCTCGTAGTGTTCCGGGAACGGACCTTCCGCCATCTTATCGAGGGCAAACAGACGCCCCATGCCtt            >  1:6498779/1‑93 (MQ=255)
                                                                                gTTAGTTCCCAACGGCGTTTCAAACGGCTCGTAGTGTTCCGGGAACGGACCTTCCGCCATCTTATCGAGGGCAAACAGACGCCCCATGCCTTc           <  1:221830/93‑1 (MQ=255)
                                                                                 ttAGTTCCCAACGGCGTTTCAAACGGCTCGTAGTGTTCCGGGAACGGACCTTCCGCCGTCTTATCTAGGGCAAACAGACGCCCCATGCCtttc          >  2:146767/1‑91 (MQ=18)
                                                                                 ttAGTTCCCAACGGCGTTTCAAACGGCTCGTAGTGTTCCGGGAACGGACCTTCCGCCATCTTATCGAGGGCAAACAGACGCCCCATGCCTTcc          <  2:4201264/93‑1 (MQ=255)
                                                                                 ttAGTTCCCAACGGCGTTTCAAACGGCTCGTAGTGTTCCGGGAACGGACCTTCCGCCATCTTATCGAGGGCAAACAGACGCCCCATGCCTTcc          >  2:3002988/1‑93 (MQ=255)
                                                                                 ttAGTTCCCAACGGCGTTTCAAACGGCTCGTAGTGTTCCGGGAACGGACCTTCCGCCATCTTATCGAGGGCAAACAGACGCCCCATGCCTTcc          <  1:3276773/93‑1 (MQ=255)
                                                                                  tAGTTCCCAACGGCGTTTCAAACGGCTCGTAGTGTTCCGGGAACGGACCTTCCGCCCTCTTATCGAGGGCAAACAGACGCCCCATGCCTTCct         >  1:1098601/1‑93 (MQ=255)
                                                                                    gTTCCCAGCGGCGTTTCAAACGGCTCGTAGTGTTCCGGGAACGGACCTTCCGCCATCTTATCGAGGGCAAACAGACGCCCCATGCCTTCctgc       >  1:4943380/1‑93 (MQ=255)
                                                                                    gTTCCCAACGGCGTTTCAAACGGCTCGTAGTGTTCCGGGAACGGACCTTCCGCCATCTTATCGAGGGCAAACAGACGCCCCATGCCTTCctgc       >  2:1162533/1‑93 (MQ=255)
                                                                                    gTTCCCAACGGCGTTTCAAACGGCTCGTAGTGTTCCGGGAACGGACCTTCCGCCATCTTATCGAGGGCAAACAGACGCCCCATGCCTTCctgc       >  1:7067417/1‑93 (MQ=255)
                                                                                       cccAGCGGCGTTTCAAACGGCTCGTAGTGTTCCGGGAACAGACCTTCCGCCATCTTATCGAGGGCAAACAGACGCCCCATGCCTTCCtgctgc    >  1:7514626/1‑93 (MQ=255)
                                                                                        ccAACGGCGTTTCAAACGGCTCGTAGTGTTCCGGGAACGGACCTTCCGCCATCTTATCGAGGGCAAACAGACGCCCCATGCCTTCCTGCTGCa   >  2:7453015/1‑93 (MQ=255)
                                                                                           |                                                                                          
TTATCGGCTTTACCCAATGCTTCGGCGTCGTCTTTAAAGATACGTGCAGCCGGGTTCGAGATAACGTTTGGATGCAGCGGGTTAGTTCCCAGCGGCGTTTCAAACGGCTCGTAGTGTTCCGGGAACGGACCTTCCGCCATCTTATCGAGGGCAAACAGACGCCCCATGCCTTCCTGCTGCAT  >  NC_000913/4080740‑4080921

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CTTATCGGCTTTACCCAATGCTTCGGCGTCGTCTTTAAAGATACGTGCAGCCGGGTTCGAGATAACGTTTGGATGCAGCGGGTTAGTTCCCAGCGGCGTTTCAAACGGCTC‑GTAGTGTTCCGGGAACGGACCTTCCGCCATCTTATCGAGGGCAAACAGACGCCCCATGCCTTCCTGCTGCATGAT  >  NC_000913/4080739‑4080924
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CTTATCGGCTTTACCCAATGCTTCGGCGTCGTCTTTAAAGATACGTGCAGCCGGGTTCGAGATAACGTTTGGATGCAGCGGGTTAGTTCCCAAC                                                                                               >  SRR4302542.6572193/1‑94 (MQ=60)
CTTATCGGCTTTACCCAATGCTTCGGCGTCGTCTTTAAAGATACGTGCAGCCGGGTTCGAGATAACGTTTGGATGCAGCGGGTTAGTTCCCAAC                                                                                               <  SRR4302542.6757918/94‑1 (MQ=60)
 TTATCGGCTTTACCCAATGCTTCGGCGTCGTCTTTAAAGATACGTGCAGCCGGGTTCGAGATAACGTTTGGATGCAGCGGGTTAGTTCCCAACG                                                                                              >  SRR4302542.5072943/1‑94 (MQ=60)
  TATCGGCTTTACCCAATGCTTCGGCGTCGTCTTTAAAGATACGTGCAGCCGGGTTCGAGATAACGTTTGGATGCAGCGGGTTAGTTCCCAACGG                                                                                             <  SRR4302542.5393445/94‑1 (MQ=60)
    TCGGCTTTACCCAATGCTTCGGCGTCGTCTTTAAAGATACGTGCAGCCGGGTTCGAGATAACGTTTGGATGCAGCGGGTTAGTTCCCAACGGCG                                                                                           >  SRR4302542.7519559/1‑94 (MQ=60)
    TCGGCTTTACCCAATGCTTCGGCGTCGTCTTTAAAGATACGTGCAGCCGGGTTCGAGATAACGTTTGGATGCAGCGGGTTAGTTCCCAACGGCG                                                                                           <  SRR4302542.4622007/94‑1 (MQ=60)
      GGCTTTACCCAATGCTTCGGCGTCGTCTTTAAAGATACGTGCAGCCGGGTTCGAGATAACGTTTGGATGCAGCGGGTTAGTTCCCAACGGCGTT                                                                                         <  SRR4302542.1919778/94‑1 (MQ=60)
       GCTTTACCCAATGCTTCGGCGTCGTCTTTAAAGATATGTGCAGCCGGGTTCGAGATAACGTTTGGATGCAGCGGGTTAGTTCCCAACGGCGTTT                                                                                        <  SRR4302542.4754776/94‑1 (MQ=60)
         TTTACCCAATGCTTCGGCGTCGTCTTTAAAGATACGTGCAGCCGGGTTCGAGATAACGTTTGGATGCAGCGGGTTAGTTCCCAACGGCGTTTCA                                                                                      <  SRR4302542.1441369/94‑1 (MQ=60)
           TACCCAATGCTTCGGCGTCGTCTTTAAAGATACGTGCAGCCGGGTTCGAGATAACGTTTGGATGCAGCGGGTTAGTTCCCAACGGCGTTTCAAA                                                                                    <  SRR4302542.6262408/94‑1 (MQ=60)
            ACCCAATGCTTCGGCGTCGTCTTTAAAGATACGTGCAGCCGGGTTCGAGATAACGTTTGGATGCAGCGGGTTAGTTCCCAGCGGCGTTTCAAAC                                                                                   <  SRR4302542.6213688/94‑1 (MQ=60)
            ACCCAATGCTTCGGCGTCGTCTTTAAAGATACGTGCAGCCGGGTTCGAGATAACGTTTGGATGCAGCGGGTTAGTTCCCAGCGGCGTTTCAAAC                                                                                   >  SRR4302542.7077845/1‑94 (MQ=60)
            ACCCAATGCTTCGGCGTCGTCTTTAAAGATACGTGCAGCCGGGTTCGAGATAACGTTTGGATGCAGCGGGTTAGTTCCCAACGGCGTTTCAAAC                                                                                   <  SRR4302542.3255332/94‑1 (MQ=60)
            ACCCAATGCTTCGGCGTCGTCTTTAAAGATACGTGCAGCCGGGTTCGAGATAACGTTTGGATGCAGCGGGTTAGTTCCCAACGGCGTTTCAAAC                                                                                   <  SRR4302542.6896471/94‑1 (MQ=60)
            ACCCAATGCTTCGGCGTCGTCTTTAAAGATACGTGCAGCCGGGTTCGAGATAACGTTTGGATGCAGCGGGTTAGTTCCCAACGGCGTTTCAAAC                                                                                   >  SRR4302542.5334387/1‑94 (MQ=60)
            ACCCAATGCTTCGGCGTCGTCTTTAAAGATACGTGCAGCCGGGTTCGAGATAACGTTTGGATGCAGCGGGTTAGTTCCCAACGGCGTTTCAAAC                                                                                   <  SRR4302542.6074582/94‑1 (MQ=60)
             CCCAATGCTTCGGCGTCGTCTTTAAAGATACGTGCAGCCGGGTTCGAGATAACGTTTGGATGCAGCGGGTTAGTTCCCAACGGCGTTTCAAACG                                                                                  <  SRR4302542.60339/94‑1 (MQ=60)
             CCCAATGCTTCGGCGTCGTCTTTAAAGATACGTGCAGCCGGGTTCGAGATAACGTTTGGATGCAGCGGGTTAGTTCCCAACGGCGTTTCAAACG                                                                                  >  SRR4302542.7029123/1‑94 (MQ=60)
               CAATGCTTCGGCGTCGTCTTTAAAGATACGTGCAGCCGGGTTCGAGATAACGTTTGGATGCAGCGGGTTAGTTCCCAGCGGCGTTTCAAACGGC                                                                                >  SRR4302542.2887872/1‑94 (MQ=60)
               CAATGCTTCGGCGTCGTCTTTAAAGATACGTGCAGCCGGGTTCGAGATAACGTTTGGATGCAGCGGGTTAGTTCCCAACGGCGTTTCAAACGGC                                                                                <  SRR4302542.7289270/94‑1 (MQ=60)
               CAATGCTTCGGCGTCGTCTTTAAAGATACGTGCAGCCGGGTTCGAGATAACGTTTGGATGCAGCGGGTTAGTTCCCAACGGCGTTTCAAACGGC                                                                                >  SRR4302542.4519473/1‑94 (MQ=60)
               CAATGCTTCGGCGTCGTCTTTAAAGATACGTGCAGCCGGGTTCGAGATAACGTTTGGATGCAGCGGGTTAGTTCCCAACGGCATTTCAAACGGC                                                                                >  SRR4302542.4308016/1‑94 (MQ=60)
                AATGCTTCGGCGTCGTCTTTAAAGATACGTGCAGCCGGGTTCGAGATAACGTTTGGATGCAGCGGGTTAGTTCCCAGCGGCGTTTCAAACGGCT                                                                               <  SRR4302542.4471455/94‑1 (MQ=60)
                  TGCTTCGGCGTCGTCTTTAAAGATACGTGCAGCCGGGTTCGAGATAACGTTTGGATGCAGCGGGTTAGTTCCCAACGGCGTTTCAAACGGCTC‑G                                                                            <  SRR4302542.3654628/94‑1 (MQ=60)
                      TCGGCGTCGTCTTTAAAGATACGTGCAGCCGGGTTCGAGATAACGTTTGGATGCAGCGGGTTAGTTCCCAGCGGCGTTTCAAACGGCTC‑GTAGT                                                                        <  SRR4302542.602233/94‑1 (MQ=60)
                       CGGCGTCGTCTTTAAAGATACGTGCAGCCGGGTTCGAGATAACGTTTGGATGCAGCGGGTTAGTTCCCAGCGGCGTTTCAAACGGCTC‑GTcggg                                                                       >  SRR4302542.6988277/1‑90 (MQ=60)
                       CGGCGTCGTCTTTAAAGATACGTGCAGCCGGGTTCGAGATAACGTTTGGATGCAGCGGGTTAGTTCCCAACGGCGTTTCAAACGGCTC‑GTAGTG                                                                       >  SRR4302542.2274872/1‑94 (MQ=60)
                       CGGCGTCGTCTTTAAAGATACGTGCAGCCGGGTTCGAGATAACGTTTGGATGCAGCGGGTTAGTTCCCAACGGCGTTTCAAACGGCTC‑GTAGTG                                                                       <  SRR4302542.4359382/94‑1 (MQ=60)
                        GGCGTCGTCTTTAAAGATACGTGCAGCCGGGTTCGAGATAACGTTTGGATGCAGCGGGTTAGTTCCCAACGGCGTTTCAAACGGCTC‑GTAGTGT                                                                      >  SRR4302542.3563124/1‑94 (MQ=60)
                        GGCGTCGTCTTTAAAGATACGTGCAGCCGGGTTCGAGATAACGTTTGGATGCAGCGGGTTAGTTCCCAACGGCGTTTCAAACGGCTC‑GTAGTGT                                                                      >  SRR4302542.461725/1‑94 (MQ=60)
                        GGCGTCGTCTTTAAAGATACGTGCAGCCGGGTTCGAGATAACGTTTGGATGCAGCGGGTTAGTTCCCAACGGCGTTTCAAACGGCTC‑GTAGTGT                                                                      >  SRR4302542.1479577/1‑94 (MQ=60)
                        GGCGTCGTCTTTAAAGATACGTGCAGCCGGGTTCGAGATAACGTTTGGATGCAGCGGGTTAGTTCCCAACGGCGTTTCAAACGGCTC‑GTAGAGT                                                                      >  SRR4302542.2368850/1‑94 (MQ=60)
                        GGCGTCGTCTTTAAAGATACGTGCAGCCGGGTTCGAGATAACGTTTGGATGCAGCGGGTTAGTTCCCAACGGCGTTTCAAACGGCTC‑GGAGGGT                                                                      >  SRR4302542.1045376/1‑94 (MQ=60)
                         GCGTCGTCTTTAAAGATACGTGCAGCCGGGTTCGAGATAACGTTTGGATGCAGCGGGTTAGTTCCCAACGGCGTTTCAAACGGCTC‑GTAGTGTT                                                                     <  SRR4302542.517116/94‑1 (MQ=60)
                          CGTCGTCTTTAAAGATACGTGCAGCCGGGTTCGAGATAACGTTTGGATGCAGCGGGTTAGTTCCCAGCGGCGTTTCAAACGGCTC‑GTAGTGTTC                                                                    >  SRR4302542.3212447/1‑94 (MQ=60)
                          CGTCGTCTTTAAAGATACGTGCAGCCGGGTTCGAGATAACGTTTGGATGCAGCGGGTTAGTTCCCAACGGCGTTTCAAACGGCTC‑GTAGTGTTC                                                                    <  SRR4302542.5056305/94‑1 (MQ=60)
                          CGTCGTCTTTAAAGATACGTGCAGCCGGGTTCGAGATAACGTTTGGATGCAGCGGGTTAGTTCCCAACGGCGTTTCAAACGGCTC‑GTAGTGTTC                                                                    >  SRR4302542.4593117/1‑94 (MQ=60)
                          CGTCGTCTTTAAAGATACGTGCAGCCGGGTTCGAGATAACGTTTGGATGCAGCGGGTTAGTTCCCAACGGCGTTTCAAACGGCTC‑GTAGTGTTC                                                                    >  SRR4302542.6520015/1‑94 (MQ=60)
                          CGTCGTCTTTAAAGATACGTGCAGCCGGGTTCGAGATAACGTTTGGATGCAGCGGGTTAGTTCCCAACGGCGTTTCAAACGGCTC‑GTAGTGTTC                                                                    >  SRR4302542.5301289/1‑94 (MQ=60)
                           GTCGTCTTTAAAGATACGTGCAGCCGGGTTCGAGATAACGTTTGGATGCAGCGGGTTAGTTCCCAACGGCGTTTCAAACGGCTC‑GTAGTGTTCC                                                                   <  SRR4302542.3676704/94‑1 (MQ=60)
                           GTCGTCTTTAAAGATACGTGCAGCCGGGTTCGAGATAACGTTTGGATGCAGCGGGTTAGTTCCCAACGGCGTTTCAAACGGCTC‑GTAGTGTTCC                                                                   >  SRR4302542.72898/1‑94 (MQ=60)
                           GTCGTCTTTAAAGATACGTGCAGCCGGGTTCGAGATAACGTTTGGATGCAGCGGGTTAGTTCCCAACGGCGTTTCAAACGGCTC‑GTAGTGTTCC                                                                   >  SRR4302542.1755563/1‑94 (MQ=60)
                           GTCGTCTTTAAAGATACGTGCAGCCGGGTTCGAGATAACGTTTGGATGCAGCGGGTTAGTTCCCAACGGCGTTTCAAACGGCTC‑GTAGTGTTCC                                                                   <  SRR4302542.834013/94‑1 (MQ=60)
                            GCGTCTTTAAAGATACGTGCAGCCGGGTTCGAGATAACGTTTGGATGCAGCGGGTTAGTTCCCAACGGCGTTTCAAACGGCTC‑GTAGTGTTCCG                                                                  <  SRR4302542.968740/94‑1 (MQ=60)
                             CGTCTTTAAAGATACGTGCAGCCGGGTTCGAGATAACGTTTGGATGCAGCGGGTTAGTTCCCAGCGGCGTTTCAAACGGCTC‑GTAGTGTTCCGG                                                                 >  SRR4302542.5175850/1‑94 (MQ=60)
                                 TTTAAAGATACGTGCAGCCGGGTTCGAGATAACGTTTGGATGCAGCGGGTTAGTTCCCAGCGGCGTTTCAAACGGCTC‑GTAGTGTTCCGGGAAC                                                             >  SRR4302542.1160936/1‑94 (MQ=60)
                                 TTTAAAGATACGTGCAGCCGGGTTCGAGATAACGTTTGGATGCAGCGGGTTAGTTCCCAACGGCGTTTCAAACGGCTC‑GTAGTGTTCCGGGAAC                                                             <  SRR4302542.4978628/94‑1 (MQ=60)
                                 TTTAAAGATACGTGCAGCCGGGTTCGAGATAACGTTTGGATGCAGCGGGTTAGTTCCCAACGGCGTTTCAAACGGCTC‑GTAGTGTTCCGGGAAC                                                             >  SRR4302542.1481618/1‑94 (MQ=60)
                                  TTAAAGATACGTGCAGCCGGGTTCGAGATAACGTTTGGATGCAGCGGGTTAGTTCCCAACGGCGTTTCAAACGGCTC‑GTAGTGTTCCGGGAACG                                                            <  SRR4302542.3149596/94‑1 (MQ=60)
                                   TAAAGATACGTGCAGCCGGGTTCGAGATAACGTTTGGATGCAGCGGGTTAGTTCCCAACGGCGTTTCAAACGGCTC‑GTAGTGTTCCGGGAACGG                                                           <  SRR4302542.6168610/94‑1 (MQ=60)
                                   TAAAGATACGTGCAGCCGGGTTCGAGATAACGTTTGGATGCAGCGGGTTAGTTCCCAACGGCGTTTCAAACGGCTC‑GTAGTGTTCCGGGAACGG                                                           <  SRR4302542.3742919/94‑1 (MQ=60)
                                     AAGATACGTGCAGCCGGGTTCGAGATAACGTTTGGATGCAGCGGGTTAGTTCCCAGCGGCGTTTCAAACGGCTC‑GTAGTGTTCCGGGAACGGAC                                                         >  SRR4302542.5137086/1‑94 (MQ=60)
                                     AAGATACGTGCAGCCGGGTTCGAGATAACGTTTGGATGCAGCGGGTTAGTTCCCAACGGCGTTTCAAACGGCTC‑GTAGTGTTCCGGGAACGGAC                                                         <  SRR4302542.839307/94‑1 (MQ=60)
                                     AAGATACGTGCAGCCGGGTTCGAGATAACGTTTGGATGCAGCGGGTTAGTTCCCAACGGCGTTTCAAACGGCTC‑GTAGTGTTCCGGGAACGGAC                                                         >  SRR4302542.5771245/1‑94 (MQ=60)
                                      AGATACGTGCAGCCGGGTTCGAGATAACGTTTGGATGCAGCGGGTTAGTTCCCAGCGGCGTTTCAAACGGCTC‑GTAGTGTTCCGGGAACGGACC                                                        <  SRR4302542.6230453/94‑1 (MQ=60)
                                       GATACGTGCAGCCGGGTTCGAGATAACGTTTGGATGCAGCGGGTTAGTTCCCAGCGGCGTTTCAAACGGCTC‑GTAGTGTTCCGGGAACGGACCT                                                       <  SRR4302542.1267074/94‑1 (MQ=60)
                                       GATACGTGCAGCCGGGTTCGAGATAACGTTTGGATGCAGCGGGTTAGTTCCCAACGGCGTTTCAAACGGCTC‑GTAGTGTTCCGGGAACGGACCT                                                       >  SRR4302542.133790/1‑94 (MQ=60)
                                        ATACGTGCAGCCGGGTTCGAGATAACGTTTGGATGCAGCGGGTTAGTTCCCAACGGCGTTTCAAACGGCTC‑GTAGTGTTCCGGGAACGGACCTT                                                      >  SRR4302542.5448516/1‑94 (MQ=60)
                                        ATACGTGCAGCCGGGTTCGAGATAACGTTTGGATGCAGCGGGTTAGTTCCCAACGGCGTTTCAAACGGCTC‑GTAGTGTTCCGGGAACGGACCTT                                                      <  SRR4302542.2277105/94‑1 (MQ=60)
                                        ATACGTGCAGCCGGGTTCGAGATAACGTTTGGATGCAGCGGGTTAGTTCCCAACGGCGTTTCAAACGGCTC‑GTAGTGTTCCGGGAACGGACCTT                                                      >  SRR4302542.5284813/1‑94 (MQ=60)
                                         TACGTGCAGCCGGGTTCGAGATAACGTTTGGATGCAGCGGGTTAGTTCCCAACGGCGTTTCAAACGGCTC‑GTAGTGTTCCGGGAACGGACCTTC                                                     <  SRR4302542.5668032/94‑1 (MQ=60)
                                          ACGTGCAGCCGGGTTCGAGATAACGTTTGGATGCAGCGGGTTAGTTCCCAACGGCGTTTCAAACGGCTC‑GTAGTGTTCCGGGAACGGACCTTCC                                                    <  SRR4302542.2937748/94‑1 (MQ=60)
                                          ACGTGCAGCCGGGTTCGAGATAACGTTTGGATGCAGCGGGTTAGTTCCCAACGGCGTTTCAAACGGCTC‑GTAGTGTTCCGGGAACGGACCTTCC                                                    <  SRR4302542.6867756/94‑1 (MQ=60)
                                            GTGCAGCCGGGTTCGAGATAACGTTTGGATGCAGCGGGTTAGTTCCCAACGGCGTTTCAAACGGCTC‑GTTGGGTTTCGGGAACGGACCTTCCGC                                                  >  SRR4302542.7542360/1‑94 (MQ=60)
                                             TGCAGCCGGGTTCGAGATAACGTTTGGATGCAGCGGGTTAGTTCCCAACGGCGTTTCAAACGGCTC‑CGAGTGTTCCCGGGACGGACCTTCCGCC                                                 >  SRR4302542.1917584/1‑94 (MQ=60)
                                              GCAGCCGGGTTCGAGATAACGTTTGGATGCAGCGGGTTAGTTCCCAGCGGCGTTTCAAACGGCTC‑GTAGTGTTCCGGGAACGGACCTTCCGCCA                                                >  SRR4302542.4274528/1‑94 (MQ=60)
                                               CAGCCGGGTTCGAGATAACGTTTGGATGCAGCGGGTTAGTTCCCAGCGGCGTTTCAAACGGCTC‑GTAGTGTTCCGGGAACGGACCTTCCGCCAT                                               <  SRR4302542.1515535/94‑1 (MQ=60)
                                                 GCCGGGTTCGAGATAACGTTTGGATGCAGCGGGTTAGTTCCCAACGGCGTTTCAAACGGCTC‑GTAGTGTTCCGGGAACGGACCTTCCGCCATCT                                             <  SRR4302542.3496850/94‑1 (MQ=60)
                                                 GCCGGGTTCGAGATAACGTTTGGATGCAGCGGGTTAGTTCCCAACGGCGTTTCAAACGGCTC‑GTAGTGTTCCGGGAACGGACCTTCCGCCATCT                                             <  SRR4302542.6801383/94‑1 (MQ=60)
                                                  CCGGGTTCGAGATAACGTTTGGATGCAGCGGGTTAGTTCCCAACGGCGTTTCAAACGGCTC‑GTAGTGTTCCGGGAACGGACCTTCCGCCATCTT                                            <  SRR4302542.4872921/94‑1 (MQ=60)
                                                    GGGTTCGAGATAACGTTTGGATGCAGCGGGTTAGTTCCCAACGGCGTTTCAAACGGCTC‑GTAGTGTTCCGGGAACGGACCTTCCGCCATCTTAT                                          <  SRR4302542.376022/94‑1 (MQ=60)
                                                    GGGTTCGAGATAACGTTTGGATGCAGCGGGTTAGTTCCCAACGGCGTTTCAAACGGCTC‑GTAGTGTTCCGGGAACGGACCTTCCGCCATCTTAT                                          >  SRR4302542.5993213/1‑94 (MQ=60)
                                                    GGGTTCGAGATAACGTTTGGATGCAGCGGGTTAGTTCCCAACGGCGTTTCAAACGGCTC‑GTAGTGTTCCGGGAACGGACCTTCCGCCATCTTAT                                          <  SRR4302542.3024226/94‑1 (MQ=60)
                                                     GGTTCGAGATAACGTTTGGATGCAGCGGGTTAGTTCCCAACGGCGTTTCAAACGGCTCGGTAGTGTTCCGGGAACGGACCTTCCGCCATCTTAT                                          >  SRR4302542.2432083/1‑94 (MQ=60)
                                                     GGTTCGAGATAACGTTTGGATGCAGCGGGTTAGTTCCCAACGGCGTTTCAAACGGCTC‑GTAGTGTTCCGGGGACGGACCCTCCGCCATCTTATC                                         >  SRR4302542.5004783/1‑94 (MQ=60)
                                                     GGTTCGAGATAACGTTTGGATGCAGCGGGTTAGTTCCCAACGGCGTTTCAAACGGCTC‑GTAGTGTTCCGGGAACGGACCTTCCGCCCTCTTATC                                         >  SRR4302542.3227313/1‑94 (MQ=60)
                                                     GGTTCGAGATAACGTTTGGATGCAGCGGGTTAGTTCCCAACGGCGTTTCAAACGGCTC‑GTAGTGTTCCGGGAACGGACCTTCCGCCATCTTATC                                         <  SRR4302542.3432476/94‑1 (MQ=60)
                                                      GTTCGAGATAACGTTTGGATGCAGCGGGTTAGTTCCCAGCGGCGTTTCAAACGGCTC‑GTAGTGTTCCGGGAACGGACCTTCCGCCATCTTATCG                                        >  SRR4302542.4638422/1‑94 (MQ=60)
                                                      GTTCGAGATAACGTTTGGATGCAGCGGGTTAGTTCCCAACGGCGTTTCAAACGGCTC‑GTAGTGTTCCGGGAACGGACCTTCCGCCATCTTATCG                                        <  SRR4302542.618451/94‑1 (MQ=60)
                                                        TCGAGATAACGTTTGGATGCAGCGGGTTAGTTCCCAACGGCGTTTCAAACGGCTC‑GTAGTGTTCCGGGAACGGACCTTCCGCCATCTTATCGAG                                      <  SRR4302542.1506670/94‑1 (MQ=60)
                                                         CGAGATAACGTTTGGATGCAGCGGGTTAGTTCCCAACGGCGTTTCAAACGGCTC‑GTAGTGTTCCGGGAACGGACCTTCCGCCATCTTATCGAGG                                     >  SRR4302542.5188003/1‑94 (MQ=60)
                                                         CGAGATAACGTTTGGATGCAGCGGGTTAGTTCCCAACGGCGTTTCAAACGGCTC‑GTAGTGTTCCGGGAACGGACCTTCCGCCATCTTATCGAGG                                     >  SRR4302542.5407960/1‑94 (MQ=60)
                                                          GAGATAACGTTTGGATGCAGCGGGTTAGTTCCCAACGGCGTTTCAAACGGCTC‑GTAGTGTTCCGGGAACGGACCTTCCGCCATCTTATCGAGGG                                    <  SRR4302542.1197953/94‑1 (MQ=60)
                                                          GAGATAACGTTTGGATGCAGCGGGTTAGTTCCCAACGGCGTTTCAAACGGCTC‑GTAGTGTTCCGGGAACGGACCTTCCGCCATCTTATCGAGGG                                    <  SRR4302542.3448512/94‑1 (MQ=60)
                                                           AGATAACGTTTGGATGCAGCGGGTTAGTTCCCAACGGCGTTTCAAACGGCTC‑GTAGTGTTCCGGGAACGGACCTTCCGCCATCTTATCGAGGGC                                   >  SRR4302542.1905621/1‑94 (MQ=60)
                                                            GATAACGTTTGGATGCAGCGGGTTAGTTCCCAACGGCGTTTCAAACGGCTC‑GTAGTGTTCCGGGAACGGGCCTTCCGCCATCTTATCGAGGGCA                                  >  SRR4302542.16592/1‑94 (MQ=60)
                                                            GATAACGTTTGGATGCAGCGGGTTAGTTCCCAACGGCGTTTCAAACGGCTC‑GTAGTGTTCCGGGAACGGACCTTCCGCCATCTTATCGAGGGCA                                  >  SRR4302542.1115940/1‑94 (MQ=60)
                                                            GATAACGTTTGGATGCAGCGGGTTAGTTCCCAACGGCGTTTCAAACGGCTC‑GTAGTGTTCCGGGAACGGACCTTCCGCCATCTTATCGAGGGCA                                  >  SRR4302542.5482209/1‑94 (MQ=60)
                                                             ATAACGTTTGGATGCAGCGGGTTAGTTCCCAACGGCGTTTCAAACGGCTC‑GTAGTGTTCCGGGAACGGACCTTCCGCCATCTTATCGAGGGCAA                                 <  SRR4302542.1082515/94‑1 (MQ=60)
                                                             ATAACGTTTGGATGCAGCGGGTTAGTTCCCAACGGCGTTTCAAACGGCTC‑GTAGTGTTCCGGGAACGGACCTTCCGCCATCTTATCGAGGGCAA                                 >  SRR4302542.6960998/1‑94 (MQ=60)
                                                              TAACGTTTGGATGCAGCGGGTTAGTTCCCAACGGCGTTTCAAACGGCTC‑GTAGTGTTCCGGGAACGGACCTTCCGCCATCTTATCGAGGGCAAA                                >  SRR4302542.1550556/1‑94 (MQ=60)
                                                               AACGTTTGGATGCAGCGGGTTAGTTCCCAACGGCGTTTCAAACGGCTC‑GTAGTGTTCCGGGAACGGACCTTCCGCCATCTTATCGAGGGCAAAC                               <  SRR4302542.3636342/94‑1 (MQ=60)
                                                               AACGTTTGGATGCAGCGGGTTAGTTCCCAACGGCGTTTCAAACGGCTC‑GTAGTGTTCCGGGAACGGACCTTCCGCCATCTTATCGAGGGCAAAC                               <  SRR4302542.4737445/94‑1 (MQ=60)
                                                                ACGTTTGGATGCAGCGGGTTAGTTCCCAGCGGCGTTTCAAACGGCTC‑GTAGTGTTCCGGGAACGGACCTTCCGCCATCTTATCGAGGGCAAACA                              <  SRR4302542.4106954/94‑1 (MQ=60)
                                                                ACGTTTGGATGCAGCGGGTTAGTTCCCAACGGCGTTTCAAACGGCTC‑GTAGTGTTCCGGGAACGGACCTTCCGCCATCTTATCGAGGGCAAACA                              <  SRR4302542.2708432/94‑1 (MQ=60)
                                                                 CGTTTGGATGCAGCGGGTTAGTTCCCAACGGCGTTTCAAACGGCTC‑GTAGTGTTCCGGGAACGGACCTTCCGCCATCTTATCGAGGGCAAACAG                             >  SRR4302542.1818752/1‑94 (MQ=60)
                                                                 CGTTTGGATGCAGCGGGTTAGTTCCCAACGGCGTTTCAAACGGCTC‑GTAGTGTTCCGGGAACGGACCTTCCGCCATCTTATCGAGGGCAAACAG                             >  SRR4302542.1799275/1‑94 (MQ=60)
                                                                 CGTTTGGATGCAGCGGGTTAGTTCCCAACGGCGTTTCAAACGGCTC‑GTAGTGTTCCGGGAACGGACCTTCCGCCATCTTATCGAGGGCAAACAG                             <  SRR4302542.2979850/94‑1 (MQ=60)
                                                                   TTTGGATGCAGCGGGTTAGTTCCCAACGGCGTTTCAAACGGCTC‑GTAGTGTTCCGGGAACGGACCTTCCGCCATCTTATCGAGGGCAAACAGAC                           <  SRR4302542.6021340/94‑1 (MQ=60)
                                                                   TTTGGATGCAGCGGGTTAGTTCCCAACGGCGTTTCAAACGGCTC‑GTAGTGTTCCGGGAACGGACCTTCCGCCATCTTATCGAGGGCAAACAGAC                           <  SRR4302542.6031813/94‑1 (MQ=60)
                                                                   TTTGGATGCAGCGGGTTAGTTCCCAACGGCGTTTCAAACGGCTC‑GTAGTGTTCCGGGAACGGACCTTCCGCCATCTTATCGAGGGCAAACAGAC                           <  SRR4302542.4624043/94‑1 (MQ=60)
                                                                    TTGGATGCAGCGGGTTAGTTCCCAACGGCGTTTCAAACGGCTC‑GTAGTGTTCCGGGAACGGACCTTCCGCCATCTTATCGAGGGCAAACAGACG                          <  SRR4302542.5086518/94‑1 (MQ=60)
                                                                    TTGGATGCAGCGGGTTAGTTCCCAACGGCGTTTCAAACGGCTC‑GTAGTGTTCCGGGAACGGACCTTCCGCCATCTTATCGAGGGCAAACAGACG                          <  SRR4302542.4443281/94‑1 (MQ=60)
                                                                    TTGGATGCAGCGGGTTAGTTCCCAACGGCGTTTCAAACGGCTC‑GTAGTGTTCCGGGAACGGACCTTCCGCCATCTTATCGAGGGCAAACAGACG                          >  SRR4302542.2645147/1‑94 (MQ=60)
                                                                     TGGATGCAGCGGGTTAGTTCCCAACGGCGTTTCAAACGGCTC‑GTAGTGTTCCGGGAACGGACCTTCCGCCATCTTATCGAGGGCAAACAGACGC                         >  SRR4302542.6135298/1‑94 (MQ=60)
                                                                      GGATGCAGCGGGTTAGTTCCCAACGGCGTTTCAAACGGCTC‑GTAGTGTTCCGGGAACGGACCTTCCGCCATCTTATCGAGGGCAAACAGACGCC                        >  SRR4302542.468672/1‑94 (MQ=60)
                                                                       GATGCAGCGGGTTAGTTCCCAACGGCGTTTCAAACGGCTC‑GTAGTGTTCCGGGAACGGACCTTCCGCCATCTTATCGAGGGCAAACAGACGCCC                       <  SRR4302542.4960613/94‑1 (MQ=60)
                                                                       GATGCAGCGGGTTAGTTCCCAACGGCGTTTCAAACGGCTC‑GTAGTGTTCCGGGAACGGACCTTCCGCCATCTTATCGAGGGCAAACAGACGCCC                       <  SRR4302542.7117061/94‑1 (MQ=60)
                                                                       GATGCAGCGGGTTAGTTCCCAACGGCGTTTCAAACGGCTC‑GTAGTGTTCCGGGAACGGACCTTCCGCCATCTTATCGAGGGCAAACAGACGCCC                       >  SRR4302542.5421422/1‑94 (MQ=60)
                                                                       GATGCAGCGGGTTAGTTCCCAACGGCGTTTCAAACGGCTC‑GCAGTGTTCCGGGGACGGACCTTCCGCCATCTTATCGAGGGCAAACAGACGCCC                       >  SRR4302542.3918259/1‑94 (MQ=60)
                                                                        ATGCAGCGGGTTAGTTCCCAGCGGCGTTTCAAACGGCTC‑GTAGTGTTCCGGGAACGGACCTTCCGCCATCTTATCGAGGGCAAACAGACGCCCC                      >  SRR4302542.6002802/1‑94 (MQ=60)
                                                                        ATGCAGCGGGTTAGTTCCCAACGGCGTTTCAAACGGCTC‑GTAGTGTTCCGGGAACGGACCTTCCGCCATCTTATCGAGGGCAAACAGACGCCCC                      >  SRR4302542.5732290/1‑94 (MQ=60)
                                                                        ATGCAGCGGGTTAGTTCCCAACGGCGTTTCAAACGGCTC‑GTAGTGTTCCGGGAACGGACCTTCCGCCATCTTATCGAGGGCAAACAGACGCCCC                      >  SRR4302542.512392/1‑94 (MQ=60)
                                                                          GCAGCGGGTTAGTTCCCAGCGGCGTTTCAAACGGCTC‑GTAGTGTTCCGGGAACGGACCTTCCGCCATCTTATCGAGGGCAAACAGACGCCCCAT                    <  SRR4302542.4960792/94‑1 (MQ=60)
                                                                          GCAGCGGGTTAGTTCCCAACGGCGTTTCAAACGGCTC‑GTAGTGTTCCGGGAACGGACCTTCCGCCATCTTATCGAGGGCAAACAGACGCCCCAT                    <  SRR4302542.5388570/94‑1 (MQ=60)
                                                                            AGCGGGTTAGTTCCCAACGGCGTTTCAAACGGCTC‑GTAGTGTTCCGGGAACGGACCTTCCGCCATCTTATCGAGGGCAAACAGACGCCCCATGC                  <  SRR4302542.7338608/94‑1 (MQ=60)
                                                                              CGGGTTAGTTCCCAACGGCGTTTCAAACGGCTC‑GTAGTGTTCCGGGAACGGACCTTCCGCCATCTTATCGAGGGCAAACAGACGCCCCATGCCT                <  SRR4302542.2552802/94‑1 (MQ=60)
                                                                              CGGGTTAGTTCCCAACGGCGTTTCAAACGGCTC‑GTAGTGTTCCGGGAACGGACCTTCCGCCATCTTATCGAGGGCAAACAGACGCCCCATGCCT                <  SRR4302542.4416122/94‑1 (MQ=60)
                                                                               GGGTTAGTTCCCAACGGCGTTTCAAACGGCTC‑GTAGTGTTCCGGGAACGGACCTTCCGCCATCTTATCGAGGGCAAACAGACGCCCCATGCCTT               >  SRR4302542.6562831/1‑94 (MQ=60)
                                                                                GGTTAGTTCCCAACGGCGTTTCAAACGGCTC‑GTAGGGTTCCGGGAACGGACCTTCCGCCATCTTATCGAGGGGAAACAGAAGCCCCATGCCTTC              >  SRR4302542.2578691/1‑94 (MQ=60)
                                                                                 GTTAGTTCCCAACGGCGTTTCAAACGGCTC‑GTAGTGTTCCGGGAACGGACCTTCCGCCATCTTATCGAGGGCAAACAGACGCCCCATGCCTTCC             <  SRR4302542.223748/94‑1 (MQ=60)
                                                                                 GTTAGTTCCCAACGGCGTTTCAAACGGCTC‑GTAGTGTTCCGGGAACGGACCTTCCGCCATCTTATCGAGGGCAAACAGACGCCCCATGCCTTCC             <  SRR4302542.3685408/94‑1 (MQ=60)
                                                                                  TTAGTTCCCAACGGCGTTTCAAACGGCTC‑GTAGTGTTCCGGGAACGGACCTTCCGCCCTCTTATCGAGGGCAAACAGACGCCCCATGCCTTCCT            >  SRR4302542.1108013/1‑94 (MQ=60)
                                                                                  TTAGTTCCCAACGGCGTTTCAAACGGCTC‑GTAGTGTTCCGGGAACGGACCTTCCGCCATCTTATCGAGGGCAAACAGACGCCCCATGCCTTCCT            <  SRR4302542.3307694/94‑1 (MQ=60)
                                                                                    AGTTCCCAGCGGCGTTTCAAACGGCTC‑GTAGTGTTCCGGGAACGGACCTTCCGCCATCTTATCGAGGGCAAACAGACGCCCCATGCCTTCCTGC          >  SRR4302542.4991413/1‑94 (MQ=60)
                                                                                    AGTTCCCAACGGCGTTTCAAACGGCTC‑GTAGTGTTCCGGGAACGGACCTTCCGCCATCTTATCGAGGGCAAACAGACGCCCCATGCCTTCCTGC          >  SRR4302542.6907626/1‑94 (MQ=60)
                                                                                    AGTTCCCAACGGCGTTTCAAACGGCTC‑GTAGTGTTCCGGGAACGGACCTTCCGCCATCTTATCGAGGGCAAACAGACGCCCCATGCCTTCCTGC          >  SRR4302542.7136783/1‑94 (MQ=60)
                                                                                     GTTCCCAACGGCGTTTCAAACGGCTC‑GTAGTGTTCCGGGAACGGACCTTCCGCCATCTTATCGAGGGCAAACAGACGCCCCATGCCTTCCTGCT         <  SRR4302542.1649845/94‑1 (MQ=60)
                                                                                      TTCCCAACGGCGTTTCAAACGGCTC‑GTAGTGTTCCGGGAACGGACCTTCCGCCATCTTATCGAGGGGAAACAGACGCCCCATGCCTTCCTGCTG        >  SRR4302542.3670837/1‑94 (MQ=60)
                                                                                       TCCCAGCGGCGTTTCAAACGGCTC‑GTAGTGTTCCGGGAACAGACCTTCCGCCATCTTATCGAGGGCAAACAGACGCCCCATGCCTTCCTGCTGC       >  SRR4302542.7588527/1‑94 (MQ=60)
                                                                                        CCCAACGGCGTTTCAAACGGCTC‑GTAGTGTTCCGGGAACGGACCTTCCGCCATCTTATCGAGGGCAAACAGACGCCCCATGCCTTCCTGCTGCA      <  SRR4302542.6993996/94‑1 (MQ=60)
                                                                                         CCAACGGCGTTTCAAACGGCTC‑GTAGTGTTCCGGGAACGGACCTTCCGCCATCTTATCGAGGGCAAACAGACGCCCCATGCCTTCCTGCTGCAT     <  SRR4302542.2611172/94‑1 (MQ=60)
                                                                                          CAACGGCGTTTCAAACGGCTC‑GTAGTGTTCCGGGAACGGACCTTCCGCCATCTTATCGAGGGCAAACAGACGCCCCATGCCTTCCTGCTGCATG    >  SRR4302542.5664059/1‑94 (MQ=60)
                                                                                          CAACGGCGTTTCAAACGGCTC‑GTAGTGTTCCGGGAACGGACCTTCCGCCATCTTATCGAGGGCAAACAGACGCCCCATGCCTTCCTGCTGCATG    <  SRR4302542.2422392/94‑1 (MQ=60)
                                                                                           AACGGCGTTTCAAACGGCTCGGTAGTGTTCCGGGAACGGACCTTCCGCCATCTTATCGAGGGCAAACAGACGCCCCATGCCTTCCTGCTGCATG    >  SRR4302542.3667149/1‑94 (MQ=60)
                                                                                           AACGGCGTTTCAAACGGCTC‑GTAGTGTTCCGGGAACGGACCTTCCGCCATCTTATCGAGGGCAAACAGACGCCCCATGCCTTCCTGCTGCATGA   >  SRR4302542.7148153/1‑94 (MQ=60)
                                                                                           AACGGCGTTTCAAACGGCTC‑GGAATGGTCCGGGCACGGCCCTTCCGCCACCTTATCGGGGGGAAACAGACGCCCCATGCCctcccgccgcatgg   >  SRR4302542.2845508/1‑80 (MQ=34)
                                                                                            ACGGCGTTTCAAACGGCTC‑GTAGTGTTCCGGGAACGGACCTTCCGCCATCTTATCGAGGGCAAACAGACGCCCCATGCCTTCCTGCTGCATGAT  <  SRR4302542.4230060/94‑1 (MQ=60)
                                                                                            |                                                                                              
CTTATCGGCTTTACCCAATGCTTCGGCGTCGTCTTTAAAGATACGTGCAGCCGGGTTCGAGATAACGTTTGGATGCAGCGGGTTAGTTCCCAGCGGCGTTTCAAACGGCTC‑GTAGTGTTCCGGGAACGGACCTTCCGCCATCTTATCGAGGGCAAACAGACGCCCCATGCCTTCCTGCTGCATGAT  >  NC_000913/4080739‑4080924

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑