Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1061 294.1 15065038 98.6% 14854127 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,088,793 T→C T385T (ACA→ACG)  frvB ← PTS system, fructose‑like enzyme IIBC component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,088,7930TC85.7% 646.9 / 100.6 279T385T (ACA→ACG) frvBPTS system, fructose‑like enzyme IIBC component
Reads supporting (aligned to +/- strand):  ref base T (16/24);  new base C (122/117);  total (138/141)
Fisher's exact test for biased strand distribution p-value = 2.33e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01

Only 100 of 288 total aligned reads displayed.

CACGCCGGTAATGCCGCCCGCCAGCGTATTGGCGGTAATCATCGGCAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACTGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGGCGCAATGAACGTCGCAAGACCCGCCGCG  >  NC_000913/4088703‑4088883
                                                                                          |                                                                                          
cACGCCGGTAATGCCGCCCGCCAGCGTATTGGCGGTAATCATCGGCAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACcgt                                                                                          <  1:1773322/93‑1 (MQ=255)
cACGCCGGTAATGCCGCCCGCCAGCGTATTGGCGGTAATCATCGGCAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACcgt                                                                                          <  2:6469811/93‑1 (MQ=255)
 aCGCCGGTAATGCCGCCCGCCAGCGTATTGGCGGTAATCATCGGCAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACcgtg                                                                                         >  2:4653461/1‑93 (MQ=255)
  cGCCGGTAATGCCGCCCGCCAGCGTATTGGCGGTAATCATCGGCAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACTGTgg                                                                                        <  2:5255535/93‑1 (MQ=255)
   gCCGGTAATGCCGCCCGCCAGCGTATTGGCGGTAATCATCGGCAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGc                                                                                       >  1:544541/1‑93 (MQ=255)
      ggTAATGCCGCCCGCCAGCGTATTGGCGGTAATCATCGGCAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCAcc                                                                                    <  1:5106348/93‑1 (MQ=255)
      ggTAATGCCGCCCGCCAGCGTATTGGCGGTAATCATCGGCAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCAcc                                                                                    <  1:2158577/93‑1 (MQ=255)
        tAATGCCGCCCGCCAGCGTATTGGCGGTAATCATCGGCAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGa                                                                                  >  1:6529648/1‑93 (MQ=255)
        tAATGCCGCCCGCCAGCGTATTGGCGGTAATCATCGGCAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGa                                                                                  <  1:1043262/93‑1 (MQ=255)
           tGCCGCCCGCCAGCGTATTGGCGGTAATCATCGGCAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACca                                                                               >  2:2540990/1‑93 (MQ=255)
              cgcccgccAGCGTATTGGCGGTAATCATCGGCAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACcacat                                                                            >  2:3868639/1‑92 (MQ=255)
               gcccgccAGCGTATTGGCGGTAATCATCGGCAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAAt                                                                           >  2:4397008/1‑93 (MQ=255)
                cccgccAGCGTATTGGCGGTAATCATCGGCAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATg                                                                          <  2:701829/93‑1 (MQ=255)
                  cgccAGCGTATTGGCGGTAATCATCGGCAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACTGTGGCACCGACCACAATGGc                                                                        <  1:6901543/93‑1 (MQ=255)
                   gccAGCGTATTGGCGGTAATCATCGGCAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCa                                                                       <  1:7384054/93‑1 (MQ=255)
                    ccAGCGTATTGGCGGTAATCATCGGCAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACTGTGGCACCGACCACAATGGCac                                                                      <  2:6952291/93‑1 (MQ=255)
                    ccAGCGTATTGGCGGTAATCATCGGCAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCac                                                                      >  1:4578574/1‑93 (MQ=255)
                    ccAGCGTATTGGCGGTAATCATCGGCAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCac                                                                      <  2:4263937/93‑1 (MQ=255)
                     cAGCGTATTGGCGGTAATCATCGGCAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCact                                                                     >  1:6086656/1‑93 (MQ=255)
                       gCGTATTGGCGGTAATCATCGGCAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCactac                                                                   >  1:682806/1‑93 (MQ=255)
                       gCGTATTGGCGGTAATCATCGGCAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCactac                                                                   >  2:225963/1‑93 (MQ=255)
                       gCGTATTGGCGGTAATCATCGGCAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCactac                                                                   <  1:5786436/93‑1 (MQ=255)
                        cGTATTGGCGGTAATCATCGGCAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACTGTGGCACCGACCACAATGGCactact                                                                  >  2:1561081/1‑93 (MQ=255)
                        cGTATTGGCGGTAATCATCGGCAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCactact                                                                  <  1:975948/93‑1 (MQ=255)
                        cGTATTGGCGGTAATCATCGGCAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCactact                                                                  <  2:7131624/93‑1 (MQ=255)
                         gTATTGGCGGTAATCATCGGCAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACTGTGGCACCGACCACAATGGCACTACTg                                                                 >  2:4046297/1‑93 (MQ=255)
                         gTATTGGCGGTAATCATCGGCAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACTGTGGCACCGACCACAATGGCACTACTg                                                                 <  1:2517193/93‑1 (MQ=255)
                         gTATTGGCGGTAATCATCGGCAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTg                                                                 >  2:550734/1‑93 (MQ=255)
                            ttGGCGGTAATCATCGGCAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACTGTGGCACCGACCACAATGGCACTACTGGCa                                                              >  2:5933899/1‑93 (MQ=255)
                              ggCGGTAATCATCGGCAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGc                                                            <  1:6023289/93‑1 (MQ=255)
                                 ggTAATCATCGGCAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACTGTGGCACCGACCACAATGGCACTACTGGCAGCctc                                                         <  1:4018450/93‑1 (MQ=255)
                                 ggTAATCATCGGCAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCctc                                                         <  2:4756687/93‑1 (MQ=255)
                                 ggTAATCATCGGCAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCctc                                                         >  1:2986695/1‑93 (MQ=255)
                                 ggTAATCATCGGCAACGGAGCTGCCAGAGCGT‑AGGAATAGCCGGTTCGGTTGCGACTGTGGCACCGACCACAATGGCACTACTGGCAGCctct                                                        <  1:114543/93‑1 (MQ=255)
                                  gTAATCATCGGCAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCctct                                                        >  2:4982161/1‑93 (MQ=255)
                                  gTAATCATCGGC‑ACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCtt                                                       >  1:620960/1‑93 (MQ=255)
                                    aaTCATCGGCAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCttt                                                      >  1:4213668/1‑93 (MQ=255)
                                     atcatcGGCAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACTGTGGCACCGACCACAATGGCACTACTGGCAGCCTCtttt                                                     <  1:6680850/93‑1 (MQ=255)
                                     atcatcGGCAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCtttt                                                     >  2:2675271/1‑93 (MQ=255)
                                        atcGGCAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTcc                                                  <  1:641353/93‑1 (MQ=255)
                                        atcGGCAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTcc                                                  >  1:2304310/1‑93 (MQ=255)
                                        atcGGCAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTcc                                                  >  1:1212709/1‑93 (MQ=255)
                                        atcGGCAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTcc                                                  >  2:444884/1‑93 (MQ=255)
                                          cGGCAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTg                                                <  1:5563676/93‑1 (MQ=255)
                                           ggCAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGt                                               <  2:4329549/93‑1 (MQ=255)
                                            gCAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTc                                              >  1:5871025/1‑93 (MQ=255)
                                            gCAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTc                                              <  2:2190143/93‑1 (MQ=255)
                                             cAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACTGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTcc                                             <  1:4874999/93‑2 (MQ=255)
                                              aaCGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCgg                                            <  2:1333690/93‑1 (MQ=255)
                                                cGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGt                                          >  1:1361820/1‑93 (MQ=255)
                                                 ggAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTa                                         >  2:3611239/1‑93 (MQ=255)
                                                  gAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTaa                                        >  1:3854643/1‑93 (MQ=255)
                                                  gAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTaa                                        >  2:6218919/1‑93 (MQ=255)
                                                   aGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTaaa                                       <  1:3106024/93‑1 (MQ=255)
                                                    gCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAAc                                      >  2:1131526/1‑93 (MQ=255)
                                                    gCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAAc                                      <  2:3433626/93‑1 (MQ=255)
                                                    gCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAAc                                      <  2:6278616/93‑1 (MQ=255)
                                                    gCTGCCAGAGCGCAAGGAATAGCCGGTTCGGTTGCGACTGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAAc                                      <  1:5135408/93‑1 (MQ=255)
                                                     cTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACa                                     <  1:5047033/93‑1 (MQ=255)
                                                      tGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACTGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAg                                    >  1:2061698/1‑93 (MQ=255)
                                                       gCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACTGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGt                                   <  2:5705524/93‑1 (MQ=255)
                                                       gCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGt                                   <  2:3839352/93‑1 (MQ=255)
                                                        ccAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGtt                                  <  1:4827129/93‑1 (MQ=255)
                                                         cAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGttt                                 >  1:4136966/1‑93 (MQ=255)
                                                          agagCGTAAGGAATAGCCGGTTCGGTTGCGACTGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTg                                >  2:7372747/1‑93 (MQ=255)
                                                          agagCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTg                                <  2:1373590/93‑1 (MQ=255)
                                                          agagCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTg                                >  1:4297016/1‑93 (MQ=255)
                                                           gagCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTgg                               <  2:614590/93‑1 (MQ=255)
                                                           gagCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTgg                               <  2:751314/93‑1 (MQ=255)
                                                            agCGTAAGGAATAGCCGGTTCGGTTGCGACTGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTggg                              <  1:3366776/93‑1 (MQ=255)
                                                               gTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGgcgc                           <  2:2576525/93‑1 (MQ=255)
                                                                tAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGGCGCa                          <  2:2057157/93‑1 (MQ=255)
                                                                tAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGGCGCa                          >  2:3736709/1‑93 (MQ=255)
                                                                 aaGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCCCTTTTTCCTGTCGGGTAAACAGTTTGGGCGCaa                         <  2:1820048/93‑1 (MQ=255)
                                                                  aGGAATAGCCGGTTCGGTTGCGACTGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGGCGCAAt                        <  1:4434518/93‑1 (MQ=255)
                                                                  aGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGGCGCAAt                        >  1:3903434/1‑93 (MQ=255)
                                                                  aGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGGCGCAAt                        <  1:5309414/93‑1 (MQ=255)
                                                                     aaTAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGGCGCAATGaa                     <  2:4904354/93‑1 (MQ=255)
                                                                     aaTAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGGCGCAATGaa                     <  2:5459336/93‑1 (MQ=255)
                                                                       tAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGGCGCAATGAACg                   >  1:2186720/1‑93 (MQ=255)
                                                                       tAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGGCGCAATGAACg                   <  1:1957143/93‑1 (MQ=255)
                                                                       tAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGGCGCAATGAACg                   >  2:659251/1‑93 (MQ=255)
                                                                         gCCGGTTCGGTTGCGACTGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGGCGCAATGAACGTc                 <  2:87779/93‑1 (MQ=255)
                                                                         gCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGGCGCAATGAACGTc                 <  2:3309557/93‑1 (MQ=255)
                                                                          ccGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGGCGCAATGAACGTCg                >  1:7046594/1‑93 (MQ=255)
                                                                            ggttcggttGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGGCGCAATGAACGTCGCa              >  2:7248775/1‑93 (MQ=255)
                                                                             gttcggttGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGGCGCAATGAACGTCGCaa             <  2:4700202/93‑1 (MQ=255)
                                                                             gttcggttGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGGCGCAATGAACGTCGCaa             <  1:2374795/93‑1 (MQ=255)
                                                                             gttcggttGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGGCGCAATGAACGTCGCaa             >  1:1845471/1‑93 (MQ=255)
                                                                             gttcggttGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGGCGCAATGAACGTCGCaa             >  2:5859242/1‑93 (MQ=255)
                                                                             gttcggttGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGGCGCAATGAACGTCGCaa             >  2:2805408/1‑93 (MQ=255)
                                                                              ttcggttGCGACTGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGGCGCAATGAACGTCGCAAg            >  1:3436914/1‑93 (MQ=255)
                                                                              ttcggttGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGGCGCAATGAACGTCGCAAg            <  1:7133371/93‑1 (MQ=255)
                                                                                cggttGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGGCGCAATGAACGTCGCAAGAc          <  1:6756213/93‑1 (MQ=255)
                                                                                 ggttGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGGCGCAATGAACGTCGCAAGAcc         <  2:5083435/93‑1 (MQ=255)
                                                                                  gttGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGGCGCAATGAACGTCGCAAGAccc        <  2:5378717/93‑1 (MQ=255)
                                                                                   ttGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGGCGCAATGAACGTCGCAAGACccg       >  2:3018647/1‑93 (MQ=255)
                                                                                        aCTGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGGCGCAATGAACGTCGCAAGACCCGCcgcg  >  2:1407443/1‑93 (MQ=255)
                                                                                        aCTGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGGCGCAATGAACGTCGCAAGACCCGCcgcg  <  2:1207827/93‑1 (MQ=255)
                                                                                        aCTGTGGCACCGACCACAATGGCACTACTGGCAGCCTCCTTTTCCTGTCGGGTAAACAGTTTGGGCGCAATGAACGTCGCAAGACCCGCcgcg  >  2:3198424/1‑93 (MQ=255)
                                                                                          |                                                                                          
CACGCCGGTAATGCCGCCCGCCAGCGTATTGGCGGTAATCATCGGCAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACTGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGGCGCAATGAACGTCGCAAGACCCGCCGCG  >  NC_000913/4088703‑4088883

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CAGCACGCCGGTAATGCCGCCCGCCAGCGTATTGGCGGTAATCATCGGCAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACTGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGGCGCAATGAACGTCGCAAGACCCGCCGCGAC  >  NC_000913/4088700‑4088885
                                                                                             |                                                                                            
CAGCACGCCGGTAATGCCGCCCGCCAGCGTATTGGCGGTAATCATCGGCAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACT                                                                                              <  SRR4302542.2235836/94‑1 (MQ=60)
CAGCACGCCGGTAATGCCGCCCGCCAGCGTATTGGCGGTAATCATCGGCAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACC                                                                                              <  SRR4302542.6384519/94‑1 (MQ=60)
 AGCACGCCGGTAATGCCGCCCGCCAGCGTATTGGCGGTAATCATCGGCAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCG                                                                                             <  SRR4302542.4686097/94‑1 (MQ=60)
   CACGCCGGTAATGCCGCCCGCCAGCGTATTGGCGGTAATCATCGGCAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTG                                                                                           >  SRR4302542.5408484/1‑94 (MQ=60)
   CACGCCGGTAATGCCGCCCGCCAGCGTATTGGCGGTAATCATCGGCAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTG                                                                                           <  SRR4302542.4102297/94‑1 (MQ=60)
   CACGCCGGTAATGCCGCCCGCCAGCGTATTGGCGGTAATCATCGGCAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTG                                                                                           <  SRR4302542.1788697/94‑1 (MQ=60)
    CCGCCGGTAATGCCGCCCGCCAGCGTATTGGCGGTAATCATCGGCAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGG                                                                                          <  SRR4302542.2362327/94‑1 (MQ=60)
    ACGCCGGTAATGCCGCCCGCCAGCGTATTGGCGGTAATCATCGGCAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGG                                                                                          >  SRR4302542.4651047/1‑94 (MQ=60)
     CGCCGGTAATGCCGCCCGCCAGCGTATTGGCGGTAATCATCGGCAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGC                                                                                         >  SRR4302542.6387465/1‑94 (MQ=60)
     CGCCGGTAATGCCGCCCGCCAGCGTATTGGCGGTAATCATCGGCAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGC                                                                                         >  SRR4302542.549240/1‑94 (MQ=60)
      GCCGGTAATGCCGCCCGCCAGCGTATTGGCGGTAATCATCGGCAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCA                                                                                        >  SRR4302542.4233262/1‑94 (MQ=60)
      GCCGGTAATGCCGCCCGCCAGCGTATTGGCGGTAATCATCGGCAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCA                                                                                        >  SRR4302542.5144949/1‑94 (MQ=60)
       CCGGTAATGCCGCCCGCCAGCGTATTGGCGGTAATCATCGGCAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACTGTGGCAC                                                                                       >  SRR4302542.4039712/1‑94 (MQ=60)
        CGGTAATGCCGCCCGCCAGCGTATTGGCGGTAATCATCGGCAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACC                                                                                      <  SRR4302542.836041/94‑1 (MQ=60)
         GGTAATGCCGCCCGCCAGCGTATTGGCGGTAATCATCGGCAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCG                                                                                     >  SRR4302542.4472252/1‑94 (MQ=60)
         GGTAATGCCGCCCGCCAGCGTATTGGCGGTAATCATCGGCAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCG                                                                                     <  SRR4302542.2177164/94‑1 (MQ=60)
         GGTAATGCCGCCCGCCAGCGTATTGGCGGTAATCATCGGCAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCG                                                                                     <  SRR4302542.5155948/94‑1 (MQ=60)
          GTAATGCCGCCCGCCAGCGTATTGGCGGTAATCATCGGCAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGA                                                                                    >  SRR4302542.6594008/1‑94 (MQ=60)
          GTAATGCCGCCCGCCAGCGTATTGGCGGTAATCATCGGCAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGA                                                                                    >  SRR4302542.1215015/1‑94 (MQ=60)
           TAATGCCGCCCGCCAGCGTATTGGCGGTAATCATCGGCAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGAC                                                                                   <  SRR4302542.1052216/94‑1 (MQ=60)
            AATGCCGCCCGCCAGCGTATTGGCGGTAATCATCGGCAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACC                                                                                  >  SRR4302542.2521404/1‑94 (MQ=60)
             ATGCCGCCCGCCAGCGTATTGGCGGTAATCATCGGCAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCA                                                                                 >  SRR4302542.191128/1‑94 (MQ=60)
                CCGCCCGCCAGCGTATTGGCGGTAATCATCGGCAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAA                                                                              >  SRR4302542.4290177/1‑94 (MQ=60)
                CCGCCCGCCAGCGTATTGGCGGTAATCATCGGCAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAA                                                                              >  SRR4302542.6134738/1‑94 (MQ=60)
                   CCCGCCAGCGTATTGGCGGTAATCATCGGCAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGG                                                                           <  SRR4302542.5034743/94‑1 (MQ=60)
                   CCCGCCAGCGTATTGGCGGTAATCATCGGCAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGG                                                                           <  SRR4302542.5161014/94‑1 (MQ=60)
                   CCCGCCAGCGTATTGGCGGTAATCATCGGCAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGG                                                                           >  SRR4302542.4513469/1‑94 (MQ=60)
                    CCGCCAGCGTATTGGCGGTAATCATCGGCAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGC                                                                          <  SRR4302542.6258896/94‑1 (MQ=60)
                     CGCCAGCGTATTGGCGGTAATCATCGGCAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACTGTGGCACCGACCACAATGGCA                                                                         <  SRR4302542.6969307/94‑1 (MQ=60)
                      GCCAGCGTATTGGCGGTAATCATCGGCAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCAC                                                                        >  SRR4302542.4623366/1‑94 (MQ=60)
                      GCCAGCGTATTGGCGGTAATCATCGGCAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCAC                                                                        >  SRR4302542.5513303/1‑94 (MQ=60)
                      GCCAGCGTATTGGCGGTAATCATCGGCAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCAC                                                                        <  SRR4302542.7456341/94‑1 (MQ=60)
                      GCCAGCGTATTGGCGGTAATCATCGGCAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCAC                                                                        >  SRR4302542.400350/1‑94 (MQ=60)
                       CCAGCGTATTGGCGGTAATCATCGGCAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACT                                                                       >  SRR4302542.6145590/1‑94 (MQ=60)
                       CCAGCGTATTGGCGGTAATCATCGGCAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACT                                                                       >  SRR4302542.6145589/1‑94 (MQ=60)
                         AGCGTATTGGCGGTAATCATCGGCAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACTGTGGCACCGACCACAATGGCACTAC                                                                     >  SRR4302542.3462634/1‑94 (MQ=60)
                         AGCGTATTGGCGGTAATCATCGGCAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTAC                                                                     >  SRR4302542.2227054/1‑94 (MQ=60)
                         AGCGTATTGGCGGTAATCATCGGCAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTAC                                                                     >  SRR4302542.4495839/1‑94 (MQ=60)
                         AGCGTATTGGCGGTAATCATCGGCAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTAC                                                                     >  SRR4302542.688701/1‑94 (MQ=60)
                          GCGTATTGGCGGTAATCATCGGCAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACT                                                                    <  SRR4302542.5842613/94‑1 (MQ=60)
                          GCGTATTGGCGGTAATCATCGGCAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACT                                                                    <  SRR4302542.3183753/94‑1 (MQ=60)
                           CGTATTGGCGGTAATCATCGGCAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTG                                                                   <  SRR4302542.4373037/94‑1 (MQ=60)
                           CGTATTGGCGGTAATCATCGGCAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTG                                                                   <  SRR4302542.984358/94‑1 (MQ=60)
                            GTATTGGCGGTAATCATCGGCAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACTGTGGCACCGACCACAATGGCACTACTGG                                                                  <  SRR4302542.2541611/94‑1 (MQ=60)
                            GTATTGGCGGTAATCATCGGCAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGG                                                                  >  SRR4302542.2585131/1‑94 (MQ=60)
                            GTATTGGCGGTAATCATCGGCAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGG                                                                  >  SRR4302542.5919269/1‑94 (MQ=60)
                             TATTGGCGGTAATCATCGGCAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGC                                                                 <  SRR4302542.4135061/94‑1 (MQ=60)
                              ATTGGCGGTAATCATCGGCAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCA                                                                >  SRR4302542.7130427/1‑94 (MQ=60)
                                 GGCGGTAATCATCGGCAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCC                                                             <  SRR4302542.6081615/94‑1 (MQ=60)
                                   CGGTAATCATCGGCAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTC                                                           >  SRR4302542.3015284/1‑94 (MQ=60)
                                    GGTAATCATCGGCAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACTGTGGCACCGACCACAATGGCACTACTGGCAGCCTCT                                                          <  SRR4302542.4058105/94‑1 (MQ=60)
                                    GGTAATCATCGGCAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCT                                                          <  SRR4302542.6133679/94‑1 (MQ=60)
                                    GGTAATCATCGGCAACGGAGCTGCCAGAGCGT‑AGGAATAGCCGGTTCGGTTGCGACTGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTT                                                         <  SRR4302542.115485/94‑1 (MQ=60)
                                    GGTAATCATCGGC‑ACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTT                                                         >  SRR4302542.626302/1‑94 (MQ=60)
                                     NNAATCATCGGCAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTT                                                         <  SRR4302542.304365/94‑1 (MQ=60)
                                     GTAATCATCGGCAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTT                                                         <  SRR4302542.5575939/94‑1 (MQ=60)
                                      TAATCATCGGCAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTT                                                        >  SRR4302542.7118234/1‑94 (MQ=60)
                                      TAATCATCGGCAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTT                                                        >  SRR4302542.4255195/1‑94 (MQ=60)
                                      TAATCATCGGCAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTT                                                        <  SRR4302542.5432059/94‑1 (MQ=60)
                                        ATCATCGGCAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACTGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTT                                                      <  SRR4302542.6746666/94‑1 (MQ=60)
                                          CATCGGCAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCC                                                    >  SRR4302542.5715208/1‑94 (MQ=60)
                                          CATCGGCAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCC                                                    >  SRR4302542.1223144/1‑94 (MQ=60)
                                          CATCGGCAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCC                                                    >  SRR4302542.2150715/1‑94 (MQ=60)
                                          CATCGGCAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCC                                                    >  SRR4302542.2324102/1‑94 (MQ=60)
                                           ATCGGCAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACTGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCT                                                   >  SRR4302542.6011289/1‑94 (MQ=60)
                                           ATCGGCAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCT                                                   <  SRR4302542.646869/94‑1 (MQ=60)
                                           ATCGGCAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCT                                                   <  SRR4302542.7426499/94‑1 (MQ=60)
                                            TCGGCAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTG                                                  <  SRR4302542.4263615/94‑1 (MQ=60)
                                             CGGCAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGT                                                 <  SRR4302542.5617737/94‑1 (MQ=60)
                                             CGGCAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGT                                                 >  SRR4302542.1188630/1‑94 (MQ=60)
                                              GGCAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTC                                                <  SRR4302542.794817/94‑1 (MQ=60)
                                              GGCAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTC                                                >  SRR4302542.5927943/1‑94 (MQ=60)
                                                CAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACTGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGG                                              >  SRR4302542.6610046/1‑94 (MQ=60)
                                                CAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACTGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCCG                                              <  SRR4302542.4922445/94‑1 (MQ=60)
                                                CAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGG                                              >  SRR4302542.7414536/1‑94 (MQ=60)
                                                 AACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGG                                             >  SRR4302542.1055719/1‑94 (MQ=60)
                                                 AACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGG                                             >  SRR4302542.2456595/1‑94 (MQ=60)
                                                 AACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGG                                             <  SRR4302542.3006166/94‑1 (MQ=60)
                                                  ACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGT                                            >  SRR4302542.1373597/1‑94 (MQ=60)
                                                    GGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAA                                          >  SRR4302542.3892721/1‑94 (MQ=60)
                                                      AGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAAC                                        <  SRR4302542.3135549/94‑1 (MQ=60)
                                                      AGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAAC                                        >  SRR4302542.6951882/1‑94 (MQ=60)
                                                      AGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAAC                                        >  SRR4302542.3927/1‑94 (MQ=60)
                                                      AGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAAC                                        >  SRR4302542.6940075/1‑94 (MQ=60)
                                                       GCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACA                                       <  SRR4302542.5199926/94‑1 (MQ=60)
                                                       GCTGCCAGAGCGCAAGGAATAGCCGGTTCGGTTGCGACTGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACA                                       <  SRR4302542.5185328/94‑1 (MQ=60)
                                                        CTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACTGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAG                                      >  SRR4302542.2079519/1‑94 (MQ=60)
                                                        CTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAG                                      >  SRR4302542.2323074/1‑94 (MQ=60)
                                                        CTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAG                                      <  SRR4302542.5096030/94‑1 (MQ=60)
                                                        CTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAG                                      >  SRR4302542.4248642/1‑94 (MQ=60)
                                                           CCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTT                                   >  SRR4302542.673428/1‑94 (MQ=60)
                                                           CCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTT                                   >  SRR4302542.4177728/1‑94 (MQ=60)
                                                           CCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTT                                   <  SRR4302542.4874168/94‑1 (MQ=60)
                                                            CAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTG                                  >  SRR4302542.3766504/1‑94 (MQ=60)
                                                            CAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTG                                  <  SRR4302542.3025973/94‑1 (MQ=60)
                                                            CAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTG                                  >  SRR4302542.4339280/1‑94 (MQ=60)
                                                             AGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGG                                 >  SRR4302542.5779666/1‑94 (MQ=60)
                                                              GAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGG                                <  SRR4302542.5948426/94‑1 (MQ=60)
                                                              GAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGG                                <  SRR4302542.6540859/94‑1 (MQ=60)
                                                               AGCGTAAGGAATAGCCGGTTCGGTTGCGACTGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGGC                               <  SRR4302542.3398396/94‑1 (MQ=60)
                                                               AGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGGC                               <  SRR4302542.1830782/94‑1 (MQ=60)
                                                                 CGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGGCGC                             >  SRR4302542.3910721/1‑94 (MQ=60)
                                                                 CGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGGCGC                             >  SRR4302542.5159190/1‑94 (MQ=60)
                                                                    AAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGGCGCAAT                          >  SRR4302542.3941965/1‑94 (MQ=60)
                                                                    AAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGGCGCAAT                          <  SRR4302542.1372156/94‑1 (MQ=60)
                                                                    AAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGGCGCAAT                          <  SRR4302542.1993298/94‑1 (MQ=60)
                                                                     AGGAATAGCCGGTTCGGTTGCGACTGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGGCGCAATG                         <  SRR4302542.4478050/94‑1 (MQ=60)
                                                                     AGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGGCGCAATG                         <  SRR4302542.5360999/94‑1 (MQ=60)
                                                                      GGAATAGCCGGTTCGGTTGCGACTGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGGCGCAATGA                        <  SRR4302542.4896834/94‑1 (MQ=60)
                                                                        AATAGCCGGTTCGGTTGCGACTGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGGCGCAATGAAC                      <  SRR4302542.67587/94‑1 (MQ=60)
                                                                        AATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGGCGCAATGAAC                      >  SRR4302542.6973499/1‑94 (MQ=60)
                                                                        AATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGGCGCAATGAAC                      <  SRR4302542.6496189/94‑1 (MQ=60)
                                                                        AATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGGCGCAATGAAC                      <  SRR4302542.3394501/94‑1 (MQ=60)
                                                                        AATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGGCGCAATGAAC                      >  SRR4302542.1829015/1‑94 (MQ=60)
                                                                         ATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGGCGCAATGAACG                     >  SRR4302542.2205556/1‑94 (MQ=60)
                                                                          TAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGGCGCAATGAACGT                    <  SRR4302542.1974065/94‑1 (MQ=60)
                                                                           AGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGGCGCAATGAACGTC                   >  SRR4302542.1448988/1‑94 (MQ=60)
                                                                           AGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGGCGCAATGAACGTC                   <  SRR4302542.1461481/94‑1 (MQ=60)
                                                                           AGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGGCGCAATGAACGTC                   >  SRR4302542.6793993/1‑94 (MQ=60)
                                                                            GCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGGCGCAATGAACGTCG                  >  SRR4302542.7115794/1‑94 (MQ=60)
                                                                            GCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGGCGCAATGAACGTCG                  <  SRR4302542.1884029/94‑1 (MQ=60)
                                                                             CCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGGCGCAATGAACGTCGC                 >  SRR4302542.5155836/1‑94 (MQ=60)
                                                                               GGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGGCGCAATGAACGTCGCAA               >  SRR4302542.1861436/1‑94 (MQ=60)
                                                                               GGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGGCGCAATGAACGTCGCAA               >  SRR4302542.3406876/1‑94 (MQ=60)
                                                                               GGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGGCGCAATGAACGTCGCAA               >  SRR4302542.2191573/1‑94 (MQ=60)
                                                                                GTTCGGTTGCGACTGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGGCGCAATGAACGTCGCAAG              <  SRR4302542.2070625/94‑1 (MQ=60)
                                                                                GTTCGGTTGCGACTGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGGCGCAATGAACGTCGCAAG              >  SRR4302542.3469089/1‑94 (MQ=60)
                                                                                GTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGGCGCAATGAACGTCGCAAG              <  SRR4302542.7050473/94‑1 (MQ=60)
                                                                                GTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGGCGCAATGAACGTCGCAAG              <  SRR4302542.2395928/94‑1 (MQ=60)
                                                                                 TTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGGCGCAATGAACGTCGCAAGA             <  SRR4302542.7203374/94‑1 (MQ=60)
                                                                                  TCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGGCGCAATGAACGTCGCAAGAC            >  SRR4302542.5312594/1‑94 (MQ=60)
                                                                                   CGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGGCGCAATGAACGTCGCAAGACC           <  SRR4302542.6822676/94‑1 (MQ=60)
                                                                                    GGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGGCGCAATGAACGTCGCAAGACCC          <  SRR4302542.1277665/94‑1 (MQ=60)
                                                                                    GGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGGCGCAATGAACGTCGCAAGACCC          >  SRR4302542.4773568/1‑94 (MQ=60)
                                                                                       TGCGACCGTGCCCCCGACCAAAATGGCATTACTGGCAGCCTCTTTTTCCTGTGGGGTAAACAGTTGGGGCGAAATGACCGTCGCAAGTCCCGCC       <  SRR4302542.6348843/94‑1 (MQ=46)
                                                                                        GCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGGCGCAATGAACGTCGCAAGACCCGCCG      <  SRR4302542.2140559/94‑1 (MQ=60)
                                                                                         CGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGGCGCAATGAACGTCGCAAGACCCGCCGC     >  SRR4302542.5069101/1‑94 (MQ=60)
                                                                                          GACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGGCGCAATGAACGTCGCAAGACCCGCCGCG    >  SRR4302542.1319011/1‑94 (MQ=60)
                                                                                          GACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGGCGCAATGAACGTCGCAAGACCCGCCGCG    >  SRR4302542.3349102/1‑94 (MQ=60)
                                                                                          GACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGGCGCAATGAACGTCGCAAGACCCGCCGCG    >  SRR4302542.7336885/1‑94 (MQ=60)
                                                                                           ACTGTGGCCCCGCCCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGGCGCAATGAACGTCGCAAGACCCGCCGCGA   <  SRR4302542.6849374/94‑1 (MQ=60)
                                                                                           ACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGGCGCAATGAACGTCGCAAGACCCGCCGCGA   >  SRR4302542.4981561/1‑94 (MQ=60)
                                                                                            CCGTGGCCCCGCCCACAGTGCCACTACTGGCAGCCTCTTTTTCCTGTCGGATAAACAGTTTGGGCGCAATGAACGTCGCAAGCCCCGCCGCGAC  <  SRR4302542.5509289/94‑1 (MQ=60)
                                                                                             |                                                                                            
CAGCACGCCGGTAATGCCGCCCGCCAGCGTATTGGCGGTAATCATCGGCAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACTGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGGCGCAATGAACGTCGCAAGACCCGCCGCGAC  >  NC_000913/4088700‑4088885

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑