Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	1061	294.1	15065038	98.6%	14854127	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,114,335	T→C	A156A (GCA→GCG)	glpK ←	glycerol kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,114,335	0	T	C	81.2%	617.9 / 146.6	304	A156A (GCA→GCG)	glpK	glycerol kinase
Reads supporting (aligned to +/- strand): ref base T (22/35); new base C (108/139); total (130/174)
Fisher's exact test for biased strand distribution p-value = 5.53e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

Only 100 of 308 total aligned reads displayed.

GCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCG  >  NC_000913/4114243‑4114427

  gTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATTTTCCAGATAAGCCACATATCAACCGTACCAAACAGCAATTCACCACGACgcgc                                                                                            >  1:6250986/1‑93 (MQ=255)

   ttGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCa                                                                                           <  2:400302/93‑1 (MQ=255)

   ttGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGCGCa                                                                                           >  1:1104691/1‑93 (MQ=255)

   ttGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGCGCa                                                                                           >  1:6400468/1‑93 (MQ=255)

   ttGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGCGCa                                                                                           <  1:3903319/93‑1 (MQ=255)

   ttGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGCGCa                                                                                           >  2:4354333/1‑93 (MQ=255)

   ttGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGCGCa                                                                                           <  1:79523/93‑1 (MQ=255)

      gtgtAATCGGTCACATGGACACGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGCGCACGc                                                                                        <  2:6460428/93‑1 (MQ=255)

        gtAATCGGTCACATGGACACGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGCGCACGCTc                                                                                      <  2:1659378/93‑1 (MQ=255)

        gtAATCGGTCACATGGACACGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGCGCACGCTc                                                                                      >  2:3272697/1‑93 (MQ=255)

         tAATCGGTCACATGGACACGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTcg                                                                                     <  2:5434717/93‑1 (MQ=255)

          aaTCGGTCACATGGACACGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGCGCACGCTcgc                                                                                    >  1:5145147/1‑93 (MQ=255)

          aaTCGGTCACATGGACACGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGCGCACGCTcgc                                                                                    >  2:3039174/1‑93 (MQ=255)

           aTCGGTCACATGGACACGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGCGCACGCTcgcg                                                                                   >  2:1765900/1‑93 (MQ=255)

            tCGGTCACATGGACACGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCga                                                                                  <  1:382875/93‑1 (MQ=255)

            tCGGTCACATGGACACGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGCGCACGCTCGCga                                                                                  <  2:4614827/93‑1 (MQ=255)

            tCGGTCACATGGACACGGCCATGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCga                                                                                  >  2:2849776/1‑93 (MQ=255)

               gTCACATGGACACGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGCGCACGCTCGCGagag                                                                               <  2:2240138/93‑1 (MQ=255)

               gTCACATGGACACGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGCGCACGCTCGCGagag                                                                               >  1:439045/1‑93 (MQ=255)

               gTCACATGGACACGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGCGCACGCTCGCGagag                                                                               >  1:7422952/1‑93 (MQ=255)

                tCACATGGACACGGCCCTGAGTCATTTTCCAGATAAGCCACATATCAACCGTACCAAACAGCAATTCACCACGACGCGCACGCTCGCGAGAGc                                                                              <  2:3365233/93‑1 (MQ=255)

                 cacaTGGACACGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGCGCACGCTCGCGAGAGcc                                                                             <  1:2147973/93‑1 (MQ=255)

                  acaTGGACACGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCt                                                                            <  1:2336303/93‑1 (MQ=255)

                  acaTGGACACGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCt                                                                            >  1:4568834/1‑93 (MQ=255)

                  acaTGGACACGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCt                                                                            >  2:2326137/1‑93 (MQ=255)

                   caTGGACACGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCtt                                                                           <  1:6442903/93‑1 (MQ=255)

                     tGGACACGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTcc                                                                         <  1:2025020/93‑1 (MQ=255)

                     tGGACACGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTcc                                                                         >  1:1489642/1‑93 (MQ=255)

                      ggACACGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCca                                                                        <  1:33069/93‑1 (MQ=255)

                        acacGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCcaca                                                                      <  1:1542749/93‑1 (MQ=255)

                         cacGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACAt                                                                     <  2:5904790/93‑1 (MQ=255)

                         cacGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACAt                                                                     <  1:2940420/93‑1 (MQ=255)

                           cGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACATgg                                                                   <  2:3465783/93‑1 (MQ=255)

                              cccTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCg                                                                >  2:1457548/1‑93 (MQ=255)

                              cccTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCg                                                                <  2:5290080/93‑1 (MQ=255)

                               ccTGAGTCATTTTCCAGATAAGCCACATATCAACCGTACCAAACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGa                                                               >  1:4144787/1‑93 (MQ=255)

                                cTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAg                                                              >  1:4924418/1‑93 (MQ=255)

                                 tGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAgg                                                             >  1:1784787/1‑93 (MQ=255)

                                 tGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAgg                                                             >  2:6514770/1‑93 (MQ=255)

                                  gAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGa                                                            >  1:2255229/1‑93 (MQ=255)

                                  gAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGa                                                            >  1:995197/1‑93 (MQ=255)

                                  gAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGa                                                            <  2:4394925/93‑1 (MQ=255)

                                    gTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATc                                                          <  1:6664505/93‑1 (MQ=255)

                                    gTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATc                                                          <  2:2690476/93‑1 (MQ=255)

                                    gTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATc                                                          >  2:3289594/1‑93 (MQ=255)

                                    gTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATc                                                          >  2:3868389/1‑93 (MQ=255)

                                     tCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATcc                                                         <  2:6286283/93‑1 (MQ=255)

                                     tCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATcc                                                         >  1:1914797/1‑93 (MQ=255)

                                      cATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCca                                                        <  2:6658225/93‑1 (MQ=255)

                                      cATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCca                                                        <  2:6114479/93‑1 (MQ=255)

                                      cATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCca                                                        <  2:7158323/93‑1 (MQ=255)

                                       aTTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCcac                                                       <  1:3483224/93‑1 (MQ=255)

                                         tttCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCcactt                                                     <  2:6048385/93‑1 (MQ=255)

                                          ttCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCcacttc                                                    <  2:2473278/93‑1 (MQ=255)

                                          ttCCAGATAAGCCACGTATCAACCGCACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCcacttc                                                    <  1:5558673/93‑1 (MQ=255)

                                            ccAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCcacttcac                                                  <  2:377884/93‑1 (MQ=255)

                                            ccAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCcacttcac                                                  >  1:3666853/1‑93 (MQ=255)

                                            ccAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCcacttcac                                                  <  2:7376603/93‑1 (MQ=255)

                                               gATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACttt                                               >  1:4892301/1‑93 (MQ=255)

                                                aTAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTg                                              <  2:3080438/93‑1 (MQ=255)

                                                 tAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTgg                                             >  2:514416/1‑93 (MQ=255)

                                                 tAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTgg                                             >  2:6705328/1‑93 (MQ=255)

                                                 tAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTgg                                             >  2:495953/1‑93 (MQ=255)

                                                 tAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTgg                                             <  2:1724550/93‑1 (MQ=255)

                                                   aGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTg                                           >  1:7223514/1‑93 (MQ=255)

                                                   aGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTg                                           >  2:6920034/1‑93 (MQ=255)

                                                    gCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGc                                          >  1:5132414/1‑93 (MQ=255)

                                                      cacaTATCAACCGGACCAAACAGCAATTCACCACGACTCGCACGCTCGGGAGAGAATTCCACATGTTCGAGGTTCCACTTCACTTTGGGGCCa                                        >  1:1638111/1‑93 (MQ=255)

                                                      cACGTATCAACCGTACCAAACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCa                                        <  1:4235967/93‑1 (MQ=255)

                                                       aCGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAg                                       >  1:5987224/1‑93 (MQ=255)

                                                         gTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGaa                                     <  1:2891502/93‑1 (MQ=255)

                                                         gTATCAACCGTACCAAACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCTAGGATCCACTTCACTTTGGTGCCAGaa                                     >  2:70086/1‑93 (MQ=255)

                                                            tCAACCGTACCAAACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAg                                  >  1:682719/1‑93 (MQ=255)

                                                             cAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGt                                 >  1:2553793/1‑93 (MQ=255)

                                                             cAACCGTACCAAACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGt                                 <  1:5033568/93‑1 (MQ=255)

                                                               aCCGTACCAAACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTAc                               <  1:3470715/93‑1 (MQ=255)

                                                               aCCGTACCAAACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTAc                               <  1:5773274/93‑1 (MQ=255)

                                                               aCCGTACCAAACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGCGCCAGAAAAGTAc                               >  1:7182848/1‑93 (MQ=255)

                                                                ccGTACCAAACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACg                              <  1:323410/93‑1 (MQ=255)

                                                                ccGTACCAAACAGCAATCCCCCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACg                              <  1:1748644/93‑1 (MQ=255)

                                                                 cGTACCAAACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACgg                             <  2:6855866/93‑1 (MQ=255)

                                                                  gTACCAAACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACggg                            <  2:4054183/93‑1 (MQ=255)

                                                                   tACCAAACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGt                           <  2:796533/93‑1 (MQ=255)

                                                                    aCCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTc                          <  1:2391592/93‑1 (MQ=255)

                                                                      cAAACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCaa                        <  2:5536988/93‑1 (MQ=255)

                                                                       aaaCAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAAt                       <  1:698920/93‑1 (MQ=255)

                                                                         aCAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCa                     <  2:6263126/93‑1 (MQ=255)

                                                                           aGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCAcc                   >  2:4254288/1‑93 (MQ=255)

                                                                           aGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCAcc                   <  1:2621214/93‑1 (MQ=255)

                                                                            gCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCa                  >  1:752067/1‑93 (MQ=255)

                                                                              aaTTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGa                <  2:5942628/93‑1 (MQ=255)

                                                                                  caccacGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAAAAAAGTACGGGTCAATCACCAGACCgg            <  1:5305313/93‑1 (MQ=255)

                                                                                     cacGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTAt         <  2:7427721/93‑1 (MQ=255)

                                                                                     cacGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTAt         <  2:1384744/93‑1 (MQ=255)

                                                                                      acgacgTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTAtt        <  1:2458579/93‑1 (MQ=255)

                                                                                      acgacgCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTAtt        <  1:1007102/93‑1 (MQ=255)

                                                                                        gacgCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATACACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATtgc      <  2:203888/93‑1 (MQ=255)

                                                                                         acgTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATtgct     <  2:5071238/93‑1 (MQ=255)

                                                                                         acgCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATtgct     <  2:6606194/93‑1 (MQ=255)

                                                                                         acgCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATtgct     <  2:5789978/93‑1 (MQ=255)

GCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCG  >  NC_000913/4114243‑4114427

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

GGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCA‑CCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGG  >  NC_000913/4114242‑4114428

GGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCA‑CCACGACGC                                                                                               >  SRR4302542.7150920/1‑94 (MQ=60)

 GCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCA‑CCACGACGTG                                                                                              <  SRR4302542.4942804/94‑1 (MQ=60)

 GCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCA‑CCACGACGTG                                                                                              <  SRR4302542.4086954/94‑1 (MQ=60)

  CGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATTTTCCAGATAAGCCACATATCAACCGTACCAAACAGCAATTCA‑CCACGACGCGC                                                                                             >  SRR4302542.6312580/1‑94 (MQ=60)

   GTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCA‑CCACGACGTGCA                                                                                            >  SRR4302542.1784365/1‑94 (MQ=60)

   GTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCA‑CCACGACGCGCA                                                                                            >  SRR4302542.4144680/1‑94 (MQ=60)

   GTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCA‑CCACGACGCGCA                                                                                            >  SRR4302542.1114135/1‑94 (MQ=60)

   GTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCA‑CCACGACGCGCA                                                                                            >  SRR4302542.3931503/1‑94 (MQ=60)

   GTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCA‑CCACGACGCGCA                                                                                            >  SRR4302542.6463536/1‑94 (MQ=60)

    TTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCA‑CCACGACGCGCAC                                                                                           <  SRR4302542.6641695/94‑1 (MQ=60)

    TTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCA‑CCACGACGCGCAC                                                                                           <  SRR4302542.3941849/94‑1 (MQ=60)

    TTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCA‑CCACGACGCGCAC                                                                                           <  SRR4302542.80185/94‑1 (MQ=60)

    TTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCA‑CCACGACGCGCAC                                                                                           <  SRR4302542.4578776/94‑1 (MQ=60)

     TGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCA‑CCACGACGTGCACG                                                                                          >  SRR4302542.4462762/1‑94 (MQ=60)

        TGTAATCGGTCACATGGACACGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCA‑CCACGACGCGCACGCTC                                                                                       <  SRR4302542.2924595/94‑1 (MQ=60)

        TGTAATCGGTCACATGGACACGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCA‑CCACGACGCGCACGCTC                                                                                       >  SRR4302542.78104/1‑94 (MQ=60)

          TAATCGGTCACATGGACACGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCA‑CCACGACGCGCACGCTCGC                                                                                     >  SRR4302542.5195166/1‑94 (MQ=60)

            ATCGGTCACATGGACACGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCA‑CCACGACGTGCACGCTCGCGA                                                                                   >  SRR4302542.2422750/1‑94 (MQ=60)

            ATCGGTCACATGGACACGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCA‑CCACGACGCGCACGCTCGCGA                                                                                   >  SRR4302542.1904243/1‑94 (MQ=60)

             TCGGTCACATGGACACGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCA‑CCACGACGTGCACGCTCGCGAG                                                                                  <  SRR4302542.386201/94‑1 (MQ=60)

             TCGGTCACATGGACACGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCA‑CCACGACGCGCACGCTCGCGAG                                                                                  <  SRR4302542.6462430/94‑1 (MQ=60)

              CGGTCACATGGACACGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCCACGACGTGCACGCTCGCGAG                                                                                  <  SRR4302542.2384532/94‑1 (MQ=60)

              CGGTCACATGGACACGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCA‑CCACGACGCGCACGCTCGCGAGA                                                                                 <  SRR4302542.5078586/94‑1 (MQ=60)

               GGTCACATGGACACGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCA‑CCACGACGCGCACGCTCGCGAGAG                                                                                >  SRR4302542.7495587/1‑94 (MQ=60)

               GGTCACATGGACACGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCA‑CCACGACGCGCACGCTCGCGAGAG                                                                                <  SRR4302542.6984553/94‑1 (MQ=60)

               GGTCACATGGACACGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCA‑CCACGACGCGCACGCTCGCGAGAG                                                                                >  SRR4302542.442850/1‑94 (MQ=60)

                 TCACATGGACACGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCA‑CCACGACGCGCACGCTCGCGAGAGCC                                                                              >  SRR4302542.6495256/1‑94 (MQ=60)

                  CACATGGACACGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCA‑CCACGACGCGCACGCTCGCGAGAGCCT                                                                             <  SRR4302542.2166490/94‑1 (MQ=60)

                  CACATGGACACGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCA‑CCACGACGCGCACGCTCGCGAGAGCCT                                                                             >  SRR4302542.6552655/1‑94 (MQ=60)

                  CACATGGACACGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCA‑CCACGACGCGCACGCTCGCGAGAGCCT                                                                             <  SRR4302542.7232958/94‑1 (MQ=60)

                  CACATGGACACGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCA‑CCACGACGCGCACGCTCGCGAGAGCCT                                                                             >  SRR4302542.4613558/1‑94 (MQ=60)

                   ACATGGACACGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCA‑CCACGACGTGCACGCTCGCGAGAGCCTT                                                                            >  SRR4302542.7435977/1‑94 (MQ=60)

                   ACATGGACACGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCA‑CCACGACGCGCACGCTCGCGAGAGCCTT                                                                            <  SRR4302542.2356315/94‑1 (MQ=60)

                    CATGGACACGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCA‑CCACGACGTGCACGCTCGCGAGAGCCTTC                                                                           <  SRR4302542.6506352/94‑1 (MQ=60)

                    CATGGACACGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCA‑CCACGACGTGCACGCTCGCGAGAGCCTTC                                                                           >  SRR4302542.5140843/1‑94 (MQ=60)

                    CATGGACACGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCA‑CCACGACGTGCACGCTCGCGAGAGCCTTC                                                                           >  SRR4302542.5182396/1‑94 (MQ=60)

                     ATGGACACGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCA‑CCACGACGTGCACGCTCGCGAGAGCCTTCC                                                                          >  SRR4302542.1502524/1‑94 (MQ=60)

                     ATGGACACGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCA‑CCACGACGCGCACGCTCGCGAGAGCCTTCC                                                                          >  SRR4302542.3920031/1‑94 (MQ=60)

                     ATGGACACGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCA‑CCACGACGCGCACGCTCGCGAGAGCCTTCC                                                                          >  SRR4302542.7578686/1‑94 (MQ=60)

                      TGGACACGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCA‑CCACGACGTGCACGCTCGCGAGAGCCTTCCA                                                                         <  SRR4302542.2042532/94‑1 (MQ=60)

                       GGACACGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCA‑CCACGACGTGCACGCTCGCGAGAGCCTTCCAC                                                                        <  SRR4302542.33362/94‑1 (MQ=60)

                       GGACACGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCA‑CCACGACGCGCACGCTCGCGAGAGCCTTCCAC                                                                        >  SRR4302542.3790536/1‑94 (MQ=60)

                         ACACGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCA‑CCACGACGCGCACGCTCGCGAGAGCCTTCCACAT                                                                      <  SRR4302542.1556066/94‑1 (MQ=60)

                         ACACGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCA‑CCACGACGCGCACGCTCGCGAGAGCCTTCCACAT                                                                      <  SRR4302542.7294088/94‑1 (MQ=60)

                          CACGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCA‑CCACGACGCGCACGCTCGCGAGAGCCTTCCACATG                                                                     <  SRR4302542.2968580/94‑1 (MQ=60)

                           ACGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCA‑CCACGACGCGCACGCTCGCGAGAGCCTTCCACATGG                                                                    <  SRR4302542.6952828/94‑1 (MQ=60)

                             GGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCC‑CCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTC                                                                  <  SRR4302542.3406990/94‑1 (MQ=60)

                             GGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCA‑CCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTC                                                                  <  SRR4302542.3406992/94‑1 (MQ=60)

                             GGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCA‑CCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTC                                                                  >  SRR4302542.5861344/1‑94 (MQ=60)

                             GGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCA‑CCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTC                                                                  <  SRR4302542.3510227/94‑1 (MQ=60)

                              GCCCTGAGTCATTTTCCAGATAAGCCACATATCAACCGTACCAAACAGCAATTCA‑CCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCG                                                                 <  SRR4302542.2322767/94‑1 (MQ=60)

                               CCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGCACCAAACAGCAATTCA‑CCACGACGTGCCCGCTCGCGAGAGCCTTCCACATGGTCGA                                                                <  SRR4302542.5703962/94‑1 (MQ=60)

                               CCCTGAGTCATTTTCCAGATAAGCCACATATCAACCGTACCAAACAGCAATTCA‑CCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGA                                                                <  SRR4302542.1946792/94‑1 (MQ=60)

                               CCCTGAGTCATTTTCCAGATAAGCCACATATCAACCGTACCAAACAGCAATTCA‑CCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGA                                                                >  SRR4302542.4185620/1‑94 (MQ=60)

                                CCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCA‑CCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAG                                                               <  SRR4302542.7573739/94‑1 (MQ=60)

                                CCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCA‑CCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAG                                                               >  SRR4302542.4972287/1‑94 (MQ=60)

                                 CTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCA‑CCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGG                                                              >  SRR4302542.1800275/1‑94 (MQ=60)

                                 CTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCA‑CCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGG                                                              >  SRR4302542.4072550/1‑94 (MQ=60)

                                 CTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCA‑CCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGG                                                              >  SRR4302542.2063166/1‑94 (MQ=60)

                                 CTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCA‑CCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGG                                                              >  SRR4302542.6049707/1‑94 (MQ=60)

                                  TGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCA‑CCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGA                                                             <  SRR4302542.5484352/94‑1 (MQ=60)

                                  TGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCA‑CCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGA                                                             <  SRR4302542.3131149/94‑1 (MQ=60)

                                  TGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCA‑CCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGA                                                             >  SRR4302542.1003760/1‑94 (MQ=60)

                                  TGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCA‑CCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGA                                                             >  SRR4302542.2274620/1‑94 (MQ=60)

                                   GAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCA‑CCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGAT                                                            <  SRR4302542.4260325/94‑1 (MQ=60)

                                   GAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCA‑CCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGAT                                                            <  SRR4302542.1748548/94‑1 (MQ=60)

                                    AGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCA‑CCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATC                                                           <  SRR4302542.4291776/94‑1 (MQ=60)

                                    AGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCA‑CCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATC                                                           <  SRR4302542.3509244/94‑1 (MQ=60)

                                     GTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCA‑CCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCC                                                          >  SRR4302542.2616972/1‑94 (MQ=60)

                                     GTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCA‑CCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCC                                                          <  SRR4302542.6730164/94‑1 (MQ=60)

                                     GTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCA‑CCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCC                                                          >  SRR4302542.1931345/1‑94 (MQ=60)

                                     GTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCA‑CCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCC                                                          <  SRR4302542.1066045/94‑1 (MQ=60)

                                      TCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCA‑CCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCA                                                         >  SRR4302542.7088795/1‑94 (MQ=60)

                                       CATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCA‑CCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCAC                                                        >  SRR4302542.2394225/1‑94 (MQ=60)

                                        ATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCA‑CCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACT                                                       <  SRR4302542.3515812/94‑1 (MQ=60)

                                         TTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCA‑CCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTT                                                      >  SRR4302542.3126211/1‑94 (MQ=60)

                                           TTCCAGATAAGCCACGTATCAACCGCACCAAACAGCAATTCA‑CCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCA                                                    <  SRR4302542.5612680/94‑1 (MQ=60)

                                            TCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCA‑CCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCAC                                                   >  SRR4302542.3701809/1‑94 (MQ=60)

                                              CAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCA‑CCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTT                                                 >  SRR4302542.4942477/1‑94 (MQ=60)

                                              CAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCA‑CCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTT                                                 >  SRR4302542.6500301/1‑94 (MQ=60)

                                               AGATAAGCCACGTATCAACCGTACCAAACAGCAATTCA‑CCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTT                                                >  SRR4302542.4939877/1‑94 (MQ=60)

                                                GATAAGCCACGTATCAACCGTACCAAACAGCAATTCA‑CCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTG                                               <  SRR4302542.1019559/94‑1 (MQ=60)

                                                GATAAGCCACGTATCAACCGTACCAAACAGCAATTCA‑CCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTG                                               <  SRR4302542.4810221/94‑1 (MQ=60)

                                                 ATAAGCCACGTATCAACCGTACCAAACAGCAATTCA‑CCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGG                                              >  SRR4302542.4651662/1‑94 (MQ=60)

                                                 ATAAGCCACGTATCAACCGTACCAAACAGCAATTCA‑CCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGG                                              >  SRR4302542.5152615/1‑94 (MQ=60)

                                                 ATAAGCCACGTATCAACCGTACCAAACAGCAATTCA‑CCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGG                                              >  SRR4302542.419894/1‑94 (MQ=60)

                                                  TAAGCCACGTATCAACCGTACCAAACAGCAATTCA‑CCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGT                                             <  SRR4302542.981157/94‑1 (MQ=60)

                                                  TAAGCCACGTATCAACCGTACCAAACAGCAATTCA‑CCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGT                                             >  SRR4302542.2309218/1‑94 (MQ=60)

                                                   AAGCCACGTATCAACCGTACCAAACAGCAATTCA‑CCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTG                                            <  SRR4302542.2204253/94‑1 (MQ=60)

                                                   AAGCCACGTATCAACCGTACCAAACAGCAATTCA‑CCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTG                                            >  SRR4302542.7294370/1‑94 (MQ=60)

                                                   AAGCCACGTATCAACCGTACCAAACAGCAATTCA‑CCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTG                                            >  SRR4302542.1728432/1‑94 (MQ=60)

                                                   AAGCCACGTATCAACCGTACCAAACAGCAATTCA‑CCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGCG                                            <  SRR4302542.1545537/94‑1 (MQ=60)

                                                    AGCCACGTATCAACCGTACCAAACAGCAATTCA‑CCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGC                                           >  SRR4302542.196530/1‑94 (MQ=60)

                                                    AGCCACGTATCAACCGTACCAAACAGCAATTCA‑CCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGC                                           >  SRR4302542.5182304/1‑94 (MQ=60)

                                                    AGCCACGTATCAACCGTACCAAACAGCAATTCA‑CCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGC                                           >  SRR4302542.3560496/1‑94 (MQ=60)

                                                      gcacaTATCAACCGGACCAAACAGCAATTCA‑CCACGACTCGCACGCTCGGGAGAGAATTCCACATGTTCGAGGTTCCACTTCACTTTGGGGCCA                                         >  SRR4302542.1652270/6‑94 (MQ=44)

                                                       CACGTATCAACCGTACCAAACAGCAATTCA‑CCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAG                                        >  SRR4302542.6045223/1‑94 (MQ=60)

                                                       CACGTATCAACCGTACCAAACAGCAATTCA‑CCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAG                                        <  SRR4302542.4277682/94‑1 (MQ=60)

                                                          GTATCAACCGTACCAAACAGCAATTCA‑CCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAA                                     <  SRR4302542.2919265/94‑1 (MQ=60)

                                                          GTATCAACCGTACCAAACAGCAATTCA‑CCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAA                                     >  SRR4302542.5763699/1‑94 (MQ=60)

                                                            ATCAACCGTACCAAACAGCAATTCA‑CCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAG                                   >  SRR4302542.688614/1‑94 (MQ=60)

                                                             TCAACCGTACCAAACAGCAATTCA‑CCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGT                                  >  SRR4302542.2578547/1‑94 (MQ=60)

                                                             TCAACCGTACCAAACAGCAATTCA‑CCACGACGCGCACGCTCGCTAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGT                                  <  SRR4302542.7373391/94‑1 (MQ=60)

                                                             TCAACCGTACCAAACAGCAATTCA‑CCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGT                                  <  SRR4302542.3860167/94‑1 (MQ=60)

                                                             TCAACCGTACCAAACAGCAATTCA‑CCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGT                                  <  SRR4302542.1185301/94‑1 (MQ=60)

                                                              CAACCGTACCAAACAGCAATTCA‑CCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTA                                 <  SRR4302542.5082430/94‑1 (MQ=60)

                                                              CAACCGTACCAAACAGCAATTCA‑CCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTA                                 >  SRR4302542.2694108/1‑94 (MQ=60)

                                                              CAACCGTACCAAACAGCAATTCA‑CCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTA                                 <  SRR4302542.5999915/94‑1 (MQ=60)

                                                               AACCGTACCAAACAGCAATTCA‑CCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTAC                                <  SRR4302542.6754420/94‑1 (MQ=60)

                                                               AACCGTACCAAACAGCAATTCA‑CCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGCGCCAGAAAAGTAC                                >  SRR4302542.7253284/1‑94 (MQ=60)

                                                                ACCGTACCAAACAGCAATTCA‑CCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACG                               <  SRR4302542.5829316/94‑1 (MQ=60)

                                                                ACCGTACCAAACAGCAATTCA‑CCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACG                               <  SRR4302542.3503213/94‑1 (MQ=60)

                                                                ACCGTACCAAACAGCAATTCA‑CCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACG                               <  SRR4302542.1457894/94‑1 (MQ=60)

                                                                ACCGTACCAAACAGCAATTCA‑CCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACG                               >  SRR4302542.2481930/1‑94 (MQ=60)

                                                                ACCGTACCAAACAGCAATTCA‑CCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACG                               <  SRR4302542.2087475/94‑1 (MQ=60)

                                                                 CCGTACCAAACAGCAATTCA‑CCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGG                              <  SRR4302542.326220/94‑1 (MQ=60)

                                                                 CCGTACCAAACAGCAATTCA‑CCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGG                              <  SRR4302542.3302192/94‑1 (MQ=60)

                                                                 CCGTACCAAACAGCAATCCC‑CCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGG                              <  SRR4302542.1763817/94‑1 (MQ=60)

                                                                  CGTACCAAACAGCAATTCA‑CCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGG                             >  SRR4302542.768354/1‑94 (MQ=60)

                                                                    TACCAAACAGCAATTCA‑CCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTC                           <  SRR4302542.2505094/94‑1 (MQ=60)

                                                                     ACCAAACAGCAATTCA‑CCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCA                          <  SRR4302542.2414301/94‑1 (MQ=60)

                                                                     ACCAAACAGCAATTCA‑CCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCA                          >  SRR4302542.6821968/1‑94 (MQ=60)

                                                                     ACCAAACAGCAATTCA‑CCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCA                          >  SRR4302542.6053848/1‑94 (MQ=60)

                                                                      CCAAACAGCAATTCA‑CCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAA                         >  SRR4302542.7271989/1‑94 (MQ=60)

                                                                       CAAACAGCAATTCA‑CCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCANN                        >  SRR4302542.1771203/1‑94 (MQ=60)

                                                                       CAAACAGCAATTCA‑CCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAAT                        <  SRR4302542.1618271/94‑1 (MQ=60)

                                                                        AAACAGCAATTCA‑CCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATC                       <  SRR4302542.2189026/94‑1 (MQ=60)

                                                                        AAACAGCAATTCA‑CCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATC                       <  SRR4302542.704978/94‑1 (MQ=60)

                                                                         AACAGCAATTCA‑CCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCA                      >  SRR4302542.2966504/1‑94 (MQ=60)

                                                                         AACAGCAATTCA‑CCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCA                      <  SRR4302542.329216/94‑1 (MQ=60)

                                                                         AACAGCAATTCA‑CCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCA                      >  SRR4302542.5385260/1‑94 (MQ=60)

                                                                           CAGCAATTCA‑CCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACC                    <  SRR4302542.984138/94‑1 (MQ=60)

                                                                            AGCAATTCA‑CCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCA                   <  SRR4302542.2646544/94‑1 (MQ=60)

                                                                            AGCAATTCA‑CCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCA                   >  SRR4302542.758572/1‑94 (MQ=60)

                                                                             GCAATTCA‑CCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAG                  >  SRR4302542.4343753/1‑94 (MQ=60)

                                                                              CAATTCA‑CCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGA                 >  SRR4302542.6335836/1‑94 (MQ=60)

                                                                                ATTCA‑CCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACC               >  SRR4302542.263661/1‑94 (MQ=60)

                                                                                ATTCA‑CCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACC               >  SRR4302542.2760487/1‑94 (MQ=60)

                                                                                  TCA‑CCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGG             <  SRR4302542.6146680/94‑1 (MQ=60)

                                                                                   CA‑CCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAAAAAAGTACGGGTCAATCACCAGACCGGT            <  SRR4302542.5356854/94‑1 (MQ=60)

                                                                                      CCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTAT          <  SRR4302542.1613426/94‑1 (MQ=60)

                                                                                       CACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCCCCAGACCGGTATT         <  SRR4302542.3690177/94‑1 (MQ=60)

                                                                                       CACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATT         <  SRR4302542.5284917/94‑1 (MQ=60)

                                                                                       CACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATT         <  SRR4302542.1910040/94‑1 (MQ=60)

                                                                                       CACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATT         <  SRR4302542.5017119/94‑1 (MQ=60)

                                                                                        ACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTG        <  SRR4302542.2482461/94‑1 (MQ=60)

                                                                                        ACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTG        <  SRR4302542.5186335/94‑1 (MQ=60)

                                                                                        ACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTG        <  SRR4302542.2496651/94‑1 (MQ=60)

                                                                                        ACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTG        <  SRR4302542.1015781/94‑1 (MQ=60)

                                                                                          GACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCT      >  SRR4302542.5195434/1‑94 (MQ=60)

                                                                                            CGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGC    >  SRR4302542.558356/1‑94 (MQ=60)

                                                                                             GCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCG   >  SRR4302542.7089172/1‑94 (MQ=60)

                                                                                              TGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGG  <  SRR4302542.153128/94‑1 (MQ=60)

                                                                                              CGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGG  >  SRR4302542.6533851/1‑94 (MQ=60)

GGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCA‑CCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGG  >  NC_000913/4114242‑4114428

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`