Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1061 294.1 15065038 98.6% 14854127 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,171,680 A→G V309A (GTA→GCA)  coaA ← pantothenate kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,171,6800AG89.6% 742.8 / 64.1 281V309A (GTA→GCA) coaApantothenate kinase
Reads supporting (aligned to +/- strand):  ref base A (10/19);  new base G (125/125);  total (136/145)
Fisher's exact test for biased strand distribution p-value = 1.21e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

Only 100 of 281 total aligned reads displayed.

GGGCGGTGAAATATCCCTGCGTAGTGCAGAAAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTACCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATATTTTGCTTTAAGTTCAGCCAGTTGATCTCTT  >  NC_000913/4171590‑4171770
                                                                                          |                                                                                          
   cGGTGAAATATCCCTGCGTAGTGCAGAAAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCa                                                                                       <  1:7473048/93‑1 (MQ=255)
     gTGAAATATCCCTGCGTAGTGCAGAAAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATg                                                                                     >  1:2944162/1‑93 (MQ=255)
          atatCCCTGCGTAGTGCAGAAAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAg                                                                                <  1:1348942/93‑1 (MQ=255)
           tatCCCTGCGTAGTGCAGAAAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGc                                                                               <  2:1211381/93‑1 (MQ=255)
            atCCCTGCGTAGTGCAGAAAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCa                                                                              >  2:6582572/1‑93 (MQ=255)
             tCCCTGCGTAGTGCAGAAAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCAc                                                                             <  1:7229755/93‑1 (MQ=255)
              cccTGCGTAGTGCAGAAAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTACCGCATGATTAGCACt                                                                            >  1:3677712/1‑93 (MQ=255)
               ccTGCGTAGTGCAGAAAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACtt                                                                           >  1:1203533/1‑93 (MQ=255)
               ccTGCGTAGTGCAGAAAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACtt                                                                           >  2:1335061/1‑93 (MQ=255)
               ccTGCGTAGTGCAGAAAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTACCGCATGATTAGCACtt                                                                           >  2:5400266/1‑93 (MQ=255)
                cTGCGTAGTGCAGAAAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACttt                                                                          >  2:833240/1‑93 (MQ=255)
                 tGCGTAGTGCAGAAAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACtttt                                                                         <  1:4735518/93‑1 (MQ=255)
                 tGCGTAGTGCAGAAAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACtttt                                                                         >  2:7028011/1‑93 (MQ=255)
                  gCGTAGTGCAGAAAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTc                                                                        <  2:7506180/93‑1 (MQ=255)
                  gCGTAGTGCAGAAAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTc                                                                        >  1:3841515/1‑93 (MQ=255)
                    gTAGTGCAGAAAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGt                                                                      >  1:1889405/1‑93 (MQ=255)
                    gTAGTGCAGAAAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGt                                                                      >  2:6527503/1‑93 (MQ=255)
                     tAGTGCAGAAAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTc                                                                     >  2:6946115/1‑93 (MQ=255)
                     tAGTGCAGAAAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTc                                                                     >  1:1673788/1‑93 (MQ=255)
                      aGTGCAGAAAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCa                                                                    <  2:6151028/93‑1 (MQ=255)
                      aGTGCAGAAAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCa                                                                    <  1:407284/93‑1 (MQ=255)
                       gTGCAGAAAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAg                                                                   <  1:5038554/93‑1 (MQ=255)
                       gTGCAGAAAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAg                                                                   >  1:3165294/1‑93 (MQ=255)
                       gTGCAGAAAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAg                                                                   >  2:7244524/1‑93 (MQ=255)
                         gCAGAAAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGa                                                                 <  2:555605/93‑1 (MQ=255)
                          cAGAAAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGAt                                                                <  2:5925314/93‑1 (MQ=255)
                           aGAAAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGAtt                                                               >  1:7372864/1‑93 (MQ=255)
                           aGAAAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGAtt                                                               <  2:3814408/93‑1 (MQ=255)
                            gAAAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTa                                                              >  1:3806136/1‑93 (MQ=255)
                             aaaaaTAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTaa                                                             <  2:1420614/93‑1 (MQ=255)
                             aaaaaTAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTaa                                                             <  2:1507494/93‑1 (MQ=255)
                             aaaaaTAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTaa                                                             <  1:3818005/93‑1 (MQ=255)
                                aaTAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACt                                                          <  1:2062371/93‑1 (MQ=255)
                                aaTAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACt                                                          >  2:5257544/1‑93 (MQ=255)
                                aaTAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTACCGCATGATTGGCACTTTTCGTCAGGATTAAACt                                                          <  1:222338/93‑1 (MQ=255)
                                 aTAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTg                                                         <  2:6038963/93‑1 (MQ=255)
                                 aTAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTg                                                         <  1:3201697/93‑1 (MQ=255)
                                 aTAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTg                                                         <  2:7048534/93‑1 (MQ=255)
                                 aTAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTg                                                         >  1:5450566/1‑93 (MQ=255)
                                  tAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTgg                                                        <  1:6098796/93‑1 (MQ=255)
                                  tAAGAAAGGGGAGTATTAGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTgg                                                        >  1:4926485/1‑93 (MQ=255)
                                   aaGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGgc                                                       <  1:692315/93‑1 (MQ=255)
                                    aGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGgcg                                                      <  2:2727459/93‑1 (MQ=255)
                                    aGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGgcg                                                      <  2:3733189/93‑1 (MQ=255)
                                    aGAAAGGGGAGAATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGtcg                                                      >  1:2111010/1‑93 (MQ=255)
                                       aaGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGgcgcgc                                                   >  2:4805483/1‑93 (MQ=255)
                                       aaGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGgcgcgc                                                   <  1:5845964/93‑1 (MQ=255)
                                        aGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCt                                                  >  2:563974/1‑93 (MQ=255)
                                          gggAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCa                                                <  2:5046088/93‑1 (MQ=255)
                                          gggAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCa                                                <  1:5161012/93‑1 (MQ=255)
                                            gAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACg                                              <  1:4267712/93‑1 (MQ=255)
                                             aGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGa                                             <  2:3334691/93‑1 (MQ=255)
                                              gTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAg                                            <  1:4630845/93‑1 (MQ=255)
                                               tATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAgt                                           >  1:4855451/1‑93 (MQ=255)
                                                aTTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAgta                                          <  2:2062176/93‑1 (MQ=255)
                                                 ttCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAgtag                                         >  2:5832208/1‑93 (MQ=255)
                                                 ttCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTACCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAgtag                                         <  1:4137400/93‑1 (MQ=255)
                                                  tCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAgtagg                                        <  2:1807546/93‑1 (MQ=255)
                                                  tCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAgtagg                                        <  1:5720209/93‑1 (MQ=255)
                                                    gCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAgtaggta                                      >  1:7129089/1‑93 (MQ=255)
                                                    gCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAgtaggta                                      <  1:4567735/93‑1 (MQ=255)
                                                    gCTCCCCTGCAAATTATTTGCGTAGGCTAACCTCTTCTGCCGCATGATTCGCCCTTTTCGCCAGGATTAAACTGGCGCCCTCACGAgtaggta                                      <  1:1090845/93‑1 (MQ=255)
                                                     cTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAgtaggtag                                     <  1:817826/93‑1 (MQ=255)
                                                     cTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAgtagggag                                     >  1:6344281/1‑93 (MQ=255)
                                                      tCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGa                                    <  1:6692657/93‑1 (MQ=255)
                                                      tCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGa                                    >  2:233768/1‑93 (MQ=255)
                                                      tCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGa                                    >  1:4458035/1‑93 (MQ=255)
                                                      tCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGa                                    >  1:4978113/1‑93 (MQ=255)
                                                       ccccTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGaa                                   >  2:4497335/1‑93 (MQ=255)
                                                       ccccTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGaa                                   >  1:4806511/1‑93 (MQ=255)
                                                        cccTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAat                                  >  2:2947545/1‑93 (MQ=255)
                                                        cccTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAat                                  >  1:3813654/1‑93 (MQ=255)
                                                        cccTGCAAATTATTTGCGTAGTCTGACCTCTTCTACCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAat                                  <  1:5357923/93‑1 (MQ=255)
                                                           tGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATAtt                               <  2:2648250/93‑1 (MQ=255)
                                                           tGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTCTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATAtt                               >  1:3922074/1‑93 (MQ=255)
                                                            gCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATAttt                              >  2:6265254/1‑93 (MQ=255)
                                                            gCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATAttt                              >  2:364247/1‑93 (MQ=255)
                                                            gCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATAttt                              <  2:432245/93‑1 (MQ=255)
                                                             cAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATAtttt                             <  1:1165986/93‑1 (MQ=255)
                                                             cAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATAtttt                             <  2:7324363/93‑1 (MQ=255)
                                                             cAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATAtttt                             <  1:4183605/93‑1 (MQ=255)
                                                               aaTTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATATTTTGc                           <  1:3458401/93‑1 (MQ=255)
                                                                 ttattTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATATTTTGCtt                         >  1:2380951/1‑93 (MQ=255)
                                                                  tattTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATATTTTGCttt                        >  1:5287853/1‑93 (MQ=255)
                                                                  tattTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATATTTTGCttt                        >  2:4149271/1‑93 (MQ=255)
                                                                   attTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATATTTTGCTTTa                       >  2:1016957/1‑93 (MQ=255)
                                                                    tttGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATATTTTGCTTTaa                      >  1:1997831/1‑93 (MQ=255)
                                                                      tGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATATTTTGCTTTAAGt                    <  1:6029972/93‑1 (MQ=255)
                                                                        cGTAGTCTGACCTCTTCTACCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATATTTTGCTTTAAGTTc                  <  1:5678699/93‑1 (MQ=255)
                                                                         gTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATATTTTGCTTTAAGTTCa                 <  1:6526014/93‑1 (MQ=255)
                                                                              cTGACCTCTTCTACCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATATTTTGCTTTAAGTTCAGCCAg            <  1:2664183/93‑1 (MQ=255)
                                                                                gACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATATTTTGCTTTAAGTTCAGCCAGtt          <  1:1580528/93‑1 (MQ=255)
                                                                                 aCCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATATTTTGCTTTAAGTTCAGCCAGTTg         >  2:3118696/1‑93 (MQ=255)
                                                                                  ccTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATATTTTGCTTTAAGTTCAGCCAGTTGa        <  2:4716724/93‑1 (MQ=255)
                                                                                    tcttctGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATATTTTGCTTTAAGTTCAGCCAGTTGAtc      >  1:6243399/1‑93 (MQ=255)
                                                                                      ttctGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATATTTTGCTTTAAGTTCAGCCAGTTGAtctc    >  2:3580074/1‑93 (MQ=255)
                                                                                      ttctGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATATTTTGCTTTAAGTTCAGCCAGTTGAtctc    >  2:6714912/1‑93 (MQ=255)
                                                                                      ttctGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATATTTTGCTTTAAGTTCAGCCAGTTGAtctc    <  2:3239781/93‑1 (MQ=255)
                                                                                        ctACCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATATTTTGCTTTAAGTTCAGCCAGTTGATCTCtt  <  1:2734628/93‑1 (MQ=255)
                                                                                        ctACCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATATTTTGCTTTAAGTTCAGCCAGTTGATCTCtt  >  1:1786260/1‑93 (MQ=255)
                                                                                          |                                                                                          
GGGCGGTGAAATATCCCTGCGTAGTGCAGAAAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTACCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATATTTTGCTTTAAGTTCAGCCAGTTGATCTCTT  >  NC_000913/4171590‑4171770

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GATGGGCGGTGAAATATCCCTGCGTAGTGCAGAAAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTACCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATATTTTGCTTTAAGTTCAGCCAGTTGATCTCTTT  >  NC_000913/4171587‑4171771
                                                                                             |                                                                                           
GATGGGCGGTGAAATATCCCTGCGTAGTGCAGAAAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTG                                                                                             <  SRR4302542.6980583/94‑1 (MQ=60)
 ATGGGCGGTGAAATATCCCTGCGTAGTGCAGAAAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGC                                                                                            >  SRR4302542.1696810/1‑94 (MQ=60)
 ATGGGCGGTGAAATATCCCTGCGTAGTGCAGAAAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGC                                                                                            >  SRR4302542.1466272/1‑94 (MQ=60)
  TGGGCGGTGAAATATCCCTGCGTAGTGCAGAAAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCC                                                                                           >  SRR4302542.5373112/1‑94 (MQ=60)
      CGGTGAAATATCCCTGCGTAGTGCAGAAAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCAT                                                                                       <  SRR4302542.7546277/94‑1 (MQ=60)
      CGGTGAAATATCCCTGCGTAGTGCAGAAAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCAT                                                                                       >  SRR4302542.6984327/1‑94 (MQ=60)
      CGGTGAAATATCCCTGCGTAGTGCAGAAAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCAT                                                                                       <  SRR4302542.6096345/94‑1 (MQ=60)
       GGTGAAATATCCCTGCGTAGTGCAGAAAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATG                                                                                      >  SRR4302542.2407214/1‑94 (MQ=60)
       GGTGAAATATCCCTGCGTAGTGCAGAAAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATG                                                                                      >  SRR4302542.2972353/1‑94 (MQ=60)
          GAAATATCCCTGCGTAGTGCAGAAAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATT                                                                                   <  SRR4302542.2095018/94‑1 (MQ=60)
           AAATATCCCTGCGTAGTGCAGAAAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTA                                                                                  >  SRR4302542.1826376/1‑94 (MQ=60)
            AATATCCCTGCGTAGTGCAGAAAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAG                                                                                 >  SRR4302542.278002/1‑94 (MQ=60)
             ATATCCCTGCGTAGTGCAGAAAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGC                                                                                <  SRR4302542.1360592/94‑1 (MQ=60)
              TATCCCTGCGTAGTGCAGAAAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCA                                                                               >  SRR4302542.6741929/1‑94 (MQ=60)
              TATCCCTGCGTAGTGCAGAAAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCA                                                                               >  SRR4302542.484384/1‑94 (MQ=60)
               ATCCCTGCGTAGTGCAGAAAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCAC                                                                              >  SRR4302542.4929746/1‑94 (MQ=60)
               ATCCCTGCGTAGTGCAGAAAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCAC                                                                              >  SRR4302542.914555/1‑94 (MQ=60)
                TCCCTGCGTAGTGCAGAAAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACT                                                                             <  SRR4302542.7300677/94‑1 (MQ=60)
                TCCCTGCGTAGTGCAGAAAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTACCGCATGATTAGCACT                                                                             <  SRR4302542.3860872/94‑1 (MQ=60)
                TCCCTGCGTAGTGCAGAAAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTACCGCATGATTAGCACT                                                                             >  SRR4302542.3713498/1‑94 (MQ=60)
                 CCCTGCGTAGTGCAGAAAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTT                                                                            >  SRR4302542.1213883/1‑94 (MQ=60)
                   CTGCGTAGTGCAGAAAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTT                                                                          >  SRR4302542.2115995/1‑94 (MQ=60)
                   CTGCGTAGTGCAGAAAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTT                                                                          <  SRR4302542.5193566/94‑1 (MQ=60)
                   CTGCGTAGTGCAGAAAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTT                                                                          <  SRR4302542.4516693/94‑1 (MQ=60)
                    TGCGTAGTGCAGAAAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTC                                                                         <  SRR4302542.4781698/94‑1 (MQ=60)
                    TGCGTAGTGCAGAAAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTC                                                                         <  SRR4302542.6336020/94‑1 (MQ=60)
                    TGCGTAGTGCAGAAAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTC                                                                         >  SRR4302542.3879489/1‑94 (MQ=60)
                    TGCGTAGTGCAGAAAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTC                                                                         <  SRR4302542.1307083/94‑1 (MQ=60)
                     GCGTAGTGCAGAAAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCG                                                                        <  SRR4302542.1971518/94‑1 (MQ=60)
                     GCGTAGTGCAGAAAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTACCGCATGATTAGCACTTTTCG                                                                        <  SRR4302542.5193486/94‑1 (MQ=60)
                      CGTAGTGCAGAAAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGT                                                                       >  SRR4302542.1905758/1‑94 (MQ=60)
                       GTAGTGCAGAAAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTC                                                                      >  SRR4302542.1688252/1‑94 (MQ=60)
                        TAGTGCAGAAAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCA                                                                     >  SRR4302542.4230662/1‑94 (MQ=60)
                         AGTGCAGAAAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAG                                                                    >  SRR4302542.3195323/1‑94 (MQ=60)
                         AGTGCAGAAAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAG                                                                    <  SRR4302542.5876345/94‑1 (MQ=60)
                         AGTGCAGAAAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAG                                                                    <  SRR4302542.410803/94‑1 (MQ=60)
                          GTGCAGAAAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGG                                                                   <  SRR4302542.5087471/94‑1 (MQ=60)
                          GTGCAGAAAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGA                                                                   <  SRR4302542.4000351/94‑1 (MQ=60)
                          GTGCAGAAAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTACCGCATGATTAGCACTTTTCGTCAGG                                                                   <  SRR4302542.3861219/94‑1 (MQ=60)
                           TGCAGAAAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTACCGCATGATTAGCACTTTTCGTCAGGA                                                                  >  SRR4302542.6468782/1‑94 (MQ=60)
                            GCAGAAAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGAT                                                                 >  SRR4302542.5855978/1‑94 (MQ=60)
                             CAGAAAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATT                                                                >  SRR4302542.7445065/1‑94 (MQ=60)
                              AGAAAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTGGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTA                                                               >  SRR4302542.5770872/1‑94 (MQ=60)
                              AGAAAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTA                                                               >  SRR4302542.4898457/1‑94 (MQ=60)
                              AGAAAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTA                                                               >  SRR4302542.6124201/1‑94 (MQ=60)
                              AGAAAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTA                                                               >  SRR4302542.3677139/1‑94 (MQ=60)
                              AGAAAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTA                                                               >  SRR4302542.3843805/1‑94 (MQ=60)
                               GAAAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAA                                                              >  SRR4302542.7123394/1‑94 (MQ=60)
                                AAAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAA                                                             <  SRR4302542.2849336/94‑1 (MQ=60)
                                AAAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAA                                                             <  SRR4302542.1228535/94‑1 (MQ=60)
                                AAAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAA                                                             <  SRR4302542.3855764/94‑1 (MQ=60)
                                 AAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAAC                                                            <  SRR4302542.7605763/94‑1 (MQ=60)
                                  AAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACT                                                           >  SRR4302542.7553059/1‑94 (MQ=60)
                                  AAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACT                                                           <  SRR4302542.535919/94‑1 (MQ=60)
                                   AATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTG                                                          >  SRR4302542.5503518/1‑94 (MQ=60)
                                   AATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTG                                                          <  SRR4302542.2080195/94‑1 (MQ=60)
                                   AATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTACCGCATGATTGGCACTTTTCGTCAGGATTAAACTG                                                          <  SRR4302542.224264/94‑1 (MQ=60)
                                    ATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGG                                                         <  SRR4302542.3232031/94‑1 (MQ=60)
                                    ATAAGAAAGGGGAGTATTAGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGG                                                         >  SRR4302542.4974371/1‑94 (MQ=60)
                                     TAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGC                                                        <  SRR4302542.6157821/94‑1 (MQ=60)
                                      AAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCG                                                       <  SRR4302542.698301/94‑1 (MQ=60)
                                      AAGAAAGGGGAGAATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGTCG                                                       >  SRR4302542.2129229/1‑94 (MQ=60)
                                        GAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTACTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCG                                                     >  SRR4302542.7423522/1‑94 (MQ=60)
                                         AAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGC                                                    >  SRR4302542.590941/1‑94 (MQ=60)
                                          AAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCT                                                   <  SRR4302542.5902663/94‑1 (MQ=60)
                                          AAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCT                                                   <  SRR4302542.7422124/94‑1 (MQ=60)
                                           AGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTC                                                  >  SRR4302542.4247508/1‑94 (MQ=60)
                                           AGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTC                                                  <  SRR4302542.3855523/94‑1 (MQ=60)
                                           AGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTC                                                  <  SRR4302542.4658747/94‑1 (MQ=60)
                                            GGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCA                                                 <  SRR4302542.4512861/94‑1 (MQ=60)
                                             GGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCAC                                                <  SRR4302542.5211170/94‑1 (MQ=60)
                                              GGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACG                                               >  SRR4302542.4841064/1‑94 (MQ=60)
                                               GAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGA                                              <  SRR4302542.4309713/94‑1 (MQ=60)
                                                 GTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGT                                            >  SRR4302542.1970926/1‑94 (MQ=60)
                                                 GTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGT                                            >  SRR4302542.4902734/1‑94 (MQ=60)
                                                 GTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGT                                            >  SRR4302542.5234946/1‑94 (MQ=60)
                                                 GTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGT                                            >  SRR4302542.4369971/1‑94 (MQ=60)
                                                 GTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGT                                            <  SRR4302542.3844817/94‑1 (MQ=60)
                                                 GTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGT                                            <  SRR4302542.4676092/94‑1 (MQ=60)
                                                  TATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTA                                           <  SRR4302542.1429246/94‑1 (MQ=60)
                                                  TATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTA                                           <  SRR4302542.5581284/94‑1 (MQ=60)
                                                   ATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAG                                          <  SRR4302542.4647898/94‑1 (MQ=60)
                                                   ATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAG                                          <  SRR4302542.166787/94‑1 (MQ=60)
                                                   ATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAG                                          <  SRR4302542.421802/94‑1 (MQ=60)
                                                   ATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAG                                          >  SRR4302542.384053/1‑94 (MQ=60)
                                                   ATTCGCTCCCCCGCAAATTATTTTTGTAGTCTGACCTCTTCTTCCGCCTGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGGAG                                          >  SRR4302542.233740/1‑94 (MQ=60)
                                                    TTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTACCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGG                                         <  SRR4302542.4178165/94‑1 (MQ=60)
                                                     TCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGT                                        <  SRR4302542.5775733/94‑1 (MQ=60)
                                                     TCGCCCCCCT‑‑AAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAG                                      <  SRR4302542.7396530/94‑1 (MQ=60)
                                                      CGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTA                                       >  SRR4302542.4871150/1‑94 (MQ=60)
                                                      CGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTA                                       >  SRR4302542.7199054/1‑94 (MQ=60)
                                                       GCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAG                                      <  SRR4302542.4612450/94‑1 (MQ=60)
                                                       GCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGGAG                                      >  SRR4302542.6406784/1‑94 (MQ=60)
                                                       GCTCCCCTGCAAATTATTTGCGTAGGCTAACCTCTTCTGCCGCATGATTCGCCCTTTTCGCCAGGATTAAACTGGCGCCCTCACGAGTAGGTAG                                      <  SRR4302542.1100192/94‑1 (MQ=60)
                                                        CTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGA                                     >  SRR4302542.4501763/1‑94 (MQ=60)
                                                        CTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGA                                     <  SRR4302542.824894/94‑1 (MQ=60)
                                                        CTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGA                                     >  SRR4302542.4738268/1‑94 (MQ=60)
                                                        CTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGA                                     >  SRR4302542.3367884/1‑94 (MQ=60)
                                                        CTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGA                                     >  SRR4302542.5026437/1‑94 (MQ=60)
                                                         TCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAA                                    >  SRR4302542.4853334/1‑94 (MQ=60)
                                                         TCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAA                                    >  SRR4302542.4103252/1‑94 (MQ=60)
                                                         TCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAA                                    >  SRR4302542.1186459/1‑94 (MQ=60)
                                                         TCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAA                                    >  SRR4302542.2167126/1‑94 (MQ=60)
                                                         TCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAA                                    <  SRR4302542.6758624/94‑1 (MQ=60)
                                                         CCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAA                                    <  SRR4302542.6859352/94‑1 (MQ=60)
                                                          CCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAAT                                   >  SRR4302542.3851381/1‑94 (MQ=60)
                                                          CCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTACCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAAT                                   <  SRR4302542.2021666/94‑1 (MQ=60)
                                                           CCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATA                                  >  SRR4302542.5419677/1‑94 (MQ=60)
                                                           CCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTACCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATA                                  <  SRR4302542.5409999/94‑1 (MQ=60)
                                                            CCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATAT                                 >  SRR4302542.1704903/1‑94 (MQ=60)
                                                             CTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATATT                                <  SRR4302542.3817476/94‑1 (MQ=60)
                                                             CTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATATT                                >  SRR4302542.7018083/1‑94 (MQ=60)
                                                             CTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATATT                                <  SRR4302542.7481366/94‑1 (MQ=60)
                                                             CTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATATT                                >  SRR4302542.1687674/1‑94 (MQ=60)
                                                             CTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATATT                                <  SRR4302542.3937553/94‑1 (MQ=60)
                                                             CTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTCTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATATT                                >  SRR4302542.3960777/1‑94 (MQ=60)
                                                              TGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATATTT                               >  SRR4302542.4997915/1‑94 (MQ=60)
                                                                CAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATATTTTG                             >  SRR4302542.934535/1‑94 (MQ=60)
                                                                CAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATATTTTG                             <  SRR4302542.1103258/94‑1 (MQ=60)
                                                                CAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATATTTTG                             <  SRR4302542.1176031/94‑1 (MQ=60)
                                                                CAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATATTTTG                             <  SRR4302542.1204372/94‑1 (MQ=60)
                                                                CAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATATTTTG                             <  SRR4302542.4224860/94‑1 (MQ=60)
                                                                 AAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATATTTTGC                            >  SRR4302542.2398752/1‑94 (MQ=60)
                                                                 AAATTATTTGCGTAGTCTGACCTCTTCTACCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATATTTTGC                            >  SRR4302542.1846226/1‑94 (MQ=60)
                                                                  AATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATATTTTGCT                           <  SRR4302542.3490784/94‑1 (MQ=60)
                                                                  AATTATTTGCGTAGTCTGACCTCTTCTACCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATATTTTGCT                           <  SRR4302542.6229854/94‑1 (MQ=60)
                                                                   ATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATATTTTGCTT                          >  SRR4302542.2402647/1‑94 (MQ=60)
                                                                   ATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATATTTTGCTT                          >  SRR4302542.6225434/1‑94 (MQ=60)
                                                                    TTATTTGCGTAGTCTGGCCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATATTTTGCTTT                         <  SRR4302542.5024591/94‑1 (MQ=60)
                                                                    TTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATATTTTGCTTT                         >  SRR4302542.5339255/1‑94 (MQ=60)
                                                                    TTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATATTTTGCTTT                         <  SRR4302542.7545636/94‑1 (MQ=60)
                                                                      ATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATATTTTGCTTTAA                       >  SRR4302542.4144171/1‑94 (MQ=60)
                                                                      ATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATATTTTGCTTTAA                       >  SRR4302542.2015067/1‑94 (MQ=60)
                                                                      ATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATATTTTGCTTTAA                       >  SRR4302542.2697402/1‑94 (MQ=60)
                                                                      ATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATATTTTGCTTTAA                       >  SRR4302542.416296/1‑94 (MQ=60)
                                                                       TTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATATTTTGCTTTAAG                      >  SRR4302542.2605865/1‑94 (MQ=60)
                                                                        TTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATATTTTGCTTTAAGT                     <  SRR4302542.2742355/94‑1 (MQ=60)
                                                                         TGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATATTTTGCTTTAAGTT                    <  SRR4302542.6088360/94‑1 (MQ=60)
                                                                          GCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATATTTTGCTTTAAGTTC                   <  SRR4302542.5085648/94‑1 (MQ=60)
                                                                           CGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATATTTTGCTTTAAGTTCA                  <  SRR4302542.5512306/94‑1 (MQ=60)
                                                                           CGTAGTCTGACCTCTTCTACCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATATTTTGCTTTAAGTTCA                  <  SRR4302542.5733843/94‑1 (MQ=60)
                                                                            GTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATATTTTGCTTTAAGTTCAG                 <  SRR4302542.6590325/94‑1 (MQ=60)
                                                                               GTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATATTTTGCTTTAAGTTCAGCCA              <  SRR4302542.3688337/94‑1 (MQ=60)
                                                                               GTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATATTTTGCTTTAAGTTCAGCCA              <  SRR4302542.5245406/94‑1 (MQ=60)
                                                                               GTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATATTTTGCTTTAAGTTCAGCCA              <  SRR4302542.2017729/94‑1 (MQ=60)
                                                                               GTCTGACCTCTTCTCCCGCATGATTAGCCCTCTTCGGCAGGATTAAACTGGCGCGCTCACGAGTAGGCAGAATATTTTGCTTTAAGTTCAGCCA              <  SRR4302542.3195952/94‑1 (MQ=60)
                                                                                TCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATATTTTGCTTTAAGTTCAGCCAG             <  SRR4302542.4960386/94‑1 (MQ=60)
                                                                                TCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATATTTTGCTTTAAGTTCAGCCAG             <  SRR4302542.3908597/94‑1 (MQ=60)
                                                                                 CTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATATTTTGCTTTAAGTTCAGCCAGT            >  SRR4302542.3739511/1‑94 (MQ=60)
                                                                                 CTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATATTTTGCTTTAAGTTCAGCCAGT            <  SRR4302542.4369383/94‑1 (MQ=60)
                                                                                 CTGACCTCTTCTACCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATATTTTGCTTTAAGTTCAGCCAGT            <  SRR4302542.2689901/94‑1 (MQ=60)
                                                                                  TGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATATTTTGCTTTAAGTTCAGCCAGTT           <  SRR4302542.660184/94‑1 (MQ=60)
                                                                                  TGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATATTTTGCTTTAAGTTCAGCCAGTT           >  SRR4302542.3394832/1‑94 (MQ=60)
                                                                                   GACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATATTTTGCTTTAAGTTCAGCCAGTTG          <  SRR4302542.1594172/94‑1 (MQ=60)
                                                                                   GACCTCTTCTACCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATATTTTGCTTTAAGTTCAGCCAGTTG          <  SRR4302542.6587959/94‑1 (MQ=60)
                                                                                     CCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATATTTTGCTTTAAGTTCAGCCAGTTGAT        >  SRR4302542.5952298/1‑94 (MQ=60)
                                                                                     CCTCTTCTACCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATATTTTGCTTTAAGTTCAGCCAGTTGAT        >  SRR4302542.3076941/1‑94 (MQ=60)
                                                                                      CTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATATTTTGCTTTAAGTTCAGCCAGTTGATC       <  SRR4302542.7208862/94‑1 (MQ=60)
                                                                                      CTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATATTTTGCTTTAAGTTCAGCCAGTTGATC       >  SRR4302542.139687/1‑94 (MQ=60)
                                                                                      CTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATATTTTGCTTTAAGTTCAGCCAGTTGATC       >  SRR4302542.6304909/1‑94 (MQ=60)
                                                                                        CTTCTACCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATATTTTGCTTTAAGTTCAGCCAGTTGATCTC     >  SRR4302542.3207033/1‑94 (MQ=60)
                                                                                          TCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATATTTTGCTTTAAGTTCAGCCAGTTGATCTCTT   >  SRR4302542.1569065/1‑94 (MQ=60)
                                                                                          TCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATATTTTGCTTTAAGTTCAGCCAGTTGATCTCTT   <  SRR4302542.5468550/94‑1 (MQ=60)
                                                                                          TCTACCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATATTTTGCTTTAAGTTCAGCCAGTTGATCTCTT   >  SRR4302542.1801759/1‑94 (MQ=60)
                                                                                           CTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATATTTTGCTTTAAGTTCAGCCAGTTGATCTCTTT  <  SRR4302542.450010/94‑1 (MQ=60)
                                                                                           CTACCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATATTTTGCTTTAAGTTCAGCCAGTTGATCTCTTT  <  SRR4302542.2761004/94‑1 (MQ=60)
                                                                                             |                                                                                           
GATGGGCGGTGAAATATCCCTGCGTAGTGCAGAAAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTACCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATATTTTGCTTTAAGTTCAGCCAGTTGATCTCTTT  >  NC_000913/4171587‑4171771

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑