Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1061 294.1 15065038 98.6% 14854127 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,314,600 C→T R66H (CGC→CAC)  phnM ← PhnM

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,314,6000CT87.3% 678.6 / 61.1 244R66H (CGC→CAC) phnMPhnM
Reads supporting (aligned to +/- strand):  ref base C (9/22);  new base T (82/131);  total (91/153)
Fisher's exact test for biased strand distribution p-value = 4.27e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.16e-01

Only 100 of 253 total aligned reads displayed.

ACGGCATCCAGTACGGTGGTGATGCCGCTCGCCACCATCAGCGCGTCGTGGCTGCTCATCGCCGAGTGGGCAGGCCAGTCAACTTTCGGGCGCGGGGTGAAGAATTTATCCAGATTATCGGTATGCAGCTCAATCAGCCCCGGCAGCAGCCAGCCGCCTTCGCCGTCCATCGCCTCCGGCAGG  >  NC_000913/4314510‑4314692
                                                                                          |                                                                                            
aCGGCATCCAGTACGGTGGTGATGCCGCTCGCCACCATCAGCGCGTCGTGGCTGCTCATCGCCGAGTGGGCAGGCCAGTCAACTTTCGGgtgc                                                                                            <  2:6143560/93‑1 (MQ=255)
 cGGCATCCAGTACGGTGGTGATGCCGCTCGCCACCATCAGCGCGTCGTGGCTGCTCATCGCCGAGTGGGCAGGCCAGTCAACTTTCGGGtgcg                                                                                           >  1:4202698/1‑93 (MQ=255)
 cGGCATCCAGTACGGTGGTGATGCCGCTCGCCACCATCAGCGCGTCGTGGCTGCTCATCGCCGAGTGGGCAGGCCAGTCAACTTTCGGGtgcg                                                                                           >  1:3896258/1‑93 (MQ=255)
 cGGCATCCAGTACGGTGGTGATGCCGCTCGCCACCATCAGCGCGTCGTGGCTGCTCATCGCCGAGTGGGCAGGCCAGTCAACTTTCGGGcgcg                                                                                           >  2:3884419/1‑93 (MQ=255)
  ggCATCCAGTACGGTGGTGATGCCGCTCGCCACCATCAGCGCGTCGTGGCTGCTCATCGCCGAGTGGGCAGGCCAGTCAACTTTCGGGTGCgg                                                                                          <  1:2800502/93‑1 (MQ=255)
  ggCATCCAGTACGGTGGTGATGCCGCTCGCCACCATCAGCGCGTCGTGGCTGCTCATCGCCGAGTGGGCAGGCCAGTCAACTTTCGGGCGCgg                                                                                          >  1:5423212/1‑93 (MQ=255)
   gCATCCAGTACGGTGGTGATGCCGCTCGCCACCATCAGCGCGTCGTTGCTGCTCATCGCCGAGTGGGCAGGCCAGTCAACTTTCGGGCGCggg                                                                                         <  1:1289663/93‑1 (MQ=255)
   gCATCCAGTACGGTGGTGATGCCGCTCGCCACCATCAGCGCGTCGTGGCTGCTCATCGCCGAGTGGGCAGGCCAGTCAACTTTCGGGTGCggg                                                                                         <  1:2519288/93‑1 (MQ=255)
    cATCCAGTACGGTGGTGATGCCGCTCGCCACCATCAGCGCGTCGTGGCTGCTCATCGCCGAGTGGGCAGGCCAGTCAACTTTCGGGTGCgggg                                                                                        <  2:2639312/93‑1 (MQ=255)
    cATCCAGTACGGTGGTGATGCCGCTCGCCACCATCAGCGCGTCGTGGCTGCTCATCGCCGAGTGGGCAGGCCAGTCAACTTTCGGGTGCgggg                                                                                        >  2:3363295/1‑93 (MQ=255)
     aTCCAGTACGGTGGTGATGCCGCTCGCCACCATCAGCGCGTCGTGGCTGCTCATCGCCGAGTGGGCAGGCCAGTCAACTTTCGGGTGCGGGGt                                                                                       <  1:4937164/93‑1 (MQ=255)
      tCCAGTACGGTGGTGATGCCGCTCGCCACCATCAGCGCGTCGTGGCTGCTCATCGCCGAGTGGGCAGGCCAGTCAACTTTCGGGTGCGGGGTg                                                                                      >  2:6068842/1‑93 (MQ=255)
      tCCAGTACGGTGGTGATGCCGCTCGCCACCATCAGCGCGTCGTGGCTGCTCATCGCCGAGTGGGCAGGCCAGTCAACTTTCGGGTGCGGGGTg                                                                                      >  1:1131432/1‑93 (MQ=255)
        cAGTACGGTGGTGATGCCGCTCGCCACCATCAGCGCGTCGTGGCTGCTCATCGCCGAGTGGGCAGGCCAGTCAACTTTCGGGTGCGGGGTgaa                                                                                    <  1:4843882/93‑1 (MQ=255)
        cAGTACGGTGGTGATGCCGCTCGCCACCATCAGCGCGTCGTGGCTGCTCATCGCCGAGTGGGCAGGCCAGTCAACTTTCGGGTGCGGGGTgaa                                                                                    <  2:6935520/93‑1 (MQ=255)
        cAGTACGGTGGTGATGCCGCTCGCCACCATCAGCGCGTCGTGGCTGCTCATCGCCGAGTGGGCAGGCCAGTCAACTTTCGGGTGCGGGGTgaa                                                                                    <  1:2650425/93‑1 (MQ=255)
         aGTACGGTGGTGATGCCGCTCGCCACCATCAGCGCGTCGTGGCTGCTCATCGCCGAGTGGGCAGGCCAGTCAACTTTCGGGTGCGGGGCgaag                                                                                   >  1:6396761/1‑93 (MQ=255)
         aGTACGGTGGTGATGCCGCTCGCCACCATCAGCGCGTCGTGGCTGCTCATCGCCGAGTGGGCAGGCCAGTCAACTTTCGGGTGCGGGGAgaag                                                                                   <  2:1815848/93‑1 (MQ=255)
          gTACGGTGGTGATGCCGCTCGCCACCATCAGCGCGTCGTGGCTGCTCATCGCCGAGTGGGCAGGCCAGTCAACTTTCGGGTGCGGGGTgaaga                                                                                  <  1:6792598/93‑1 (MQ=255)
           tACGGTGGTGATGCCGCTCGCCACCATCAGCGCGTCGTGGCTGCTCATCGCCGAGTGGGCAGGCCAGTCAACTTTCGGGTGCGGGGTgaagaa                                                                                 >  1:1902246/1‑93 (MQ=255)
           tACGGTGGTGATGCCGCTCGCCACCATCAGCGCGTCGTGGCTGCTCATCGCCGAGTGGGCAGGCCAGTCAACTTTCGGGCGCGGGGTgaagaa                                                                                 >  2:4167531/1‑93 (MQ=255)
            aCGGTGGTGATGCCGCTCGCCACCATCAGCGCGTCGTGGCTGCTCATCGCCGAGTGGGCAGGCCAGTCAACTTTCGGGTGCGGGGTGAAGAAt                                                                                >  1:1805168/1‑93 (MQ=255)
             cGGTGGTGATGCCGCTCGCCACCATCAGCGCGTCGTGGCTGCTCATCGCCGAGTGGGCAGGCCAGTCAACTTTCGGGTGCGGGGTGAAGAAtt                                                                               <  2:5647116/93‑1 (MQ=255)
             cGGTGGTGATGCCGCTCGCCACCATCAGCGCGTCGTGGCTGCTCATCGCCGAGTGGGCAGGCCAGTCAACTTTCGGGTGCGGGGTGAAGAAtt                                                                               <  2:2803930/93‑1 (MQ=255)
               gtggtgATGCCGCTCGCCACCATCAGCGCGTCGTGGCTGCTCATCGCCGAGTGGGCAGGCCAGTCAACTTTCGGGTGCGGGGTGAAGAATTTa                                                                             >  2:1842814/1‑93 (MQ=255)
               gtggtgATGCCGCTCGCCACCATCAGCGCGTCGTGGCTGCTCATCGCCGAGTGGGCAGGCCAGTCAACTTTCGGGTGCGGGGTGAAGAATTTa                                                                             >  1:5512445/1‑93 (MQ=255)
               gtggtgATGCCGCTCGCCACCATCAGCGCGTCGTGGCTGCTCATCGCCGAGTGGGCAGGCCAGTCAACTTTCGGGCGCGGGGTGAAGAATTTa                                                                             <  2:5474847/93‑1 (MQ=255)
                tggtgATGCCGCTCGCCACCATCAGCGCGTCGTGGCTGCTCATCGCCGAGTGGGCAGGCCAGTCAACTTTCGGGTGCGGGGTGAAGAATTTAt                                                                            >  1:6227668/1‑93 (MQ=255)
                tggtgATGCCGCTCGCCACCATCAGCGCGTCGTGGCTGCTCATCGCCGAGTGGGCAGGCCAGTCAACTTTCGGGTGCGGGGTGAAGAATTTAt                                                                            >  2:2970012/1‑93 (MQ=255)
                 ggtgATGCCGCTCGCCACCATCAGCGCGTCGTGGCTGCTCATCGCCGAGTGGGCAGGCCAGTCAACTTTCGGGTGCGGGGTGAAGAATTTATc                                                                           <  2:5733876/93‑1 (MQ=255)
                  gtgATGCCGCTCGCCACCATCAGCGCGTCGTGGCTGCTCATCGCCGAGTGGGCAGGCCAGTCAACTTTCGGGTGCGGGGTGAAGAATTTATcc                                                                          <  2:3468079/93‑1 (MQ=255)
                  gtgATGCCGCTCGCCACCATCAGCGCGTCGTGGCTGCTCATCGCCGAGTGGGCAGGCCAGTCAACTTTCGGGTGCGGGGTGAAGAATTTATcc                                                                          <  1:283087/93‑1 (MQ=255)
                  gtgATGCCGCTCGCCACCATCAGCGCGTCGTGGCTGCTCATCGCCGAGTGGGCAGGCCAGTCAACTTTCGGGTGCGGGGTGAAGAATTTATcc                                                                          >  2:5040977/1‑93 (MQ=255)
                   tgATGCCGCTCGCCACCATCAGCGCGTCGTGGCTGCTCATCGCCGAGTGGGCAGGCCAGTCAACTTTCGGGTGCGGGGTGAAGAATTTATCCa                                                                         <  2:5861925/93‑1 (MQ=255)
                   tgATGCCGCTCGCCACCATCAGCGCGTCGTGGCTGCTCATCGCCGAGTGGGCAGGCCAGTCAACTTTCGGGTGCGGGGTGAAGAATTTATCCa                                                                         <  1:5066553/93‑1 (MQ=255)
                    gATGCCGCTCGCCACCATCAGCGCGTCGTGGCTGCTCATCGCCGAGTGGGCAGGCCAGTCAACTTTCGGGTGCGGGGTGAAGAATTTATCCAg                                                                        >  2:5255193/1‑93 (MQ=255)
                     aTGCCGCTCGCCACCATCAGCGCGTCGTGGCTGCTCATCGCCGAGTGGGCAGGCCAGTCAACTTTCGGGCGCGGGGTGAAGAATTTATCCAGa                                                                       <  2:1435529/93‑1 (MQ=255)
                      tGCCGCTCGCCACCATCAGCGCGTCGTGGCTGCTCATCGCCGAGTGGGCAGGCCAGTCAACTTTCGGGTGCGGGGTGAAGAATTTATCCAGAt                                                                      <  2:6315191/93‑1 (MQ=255)
                        ccGCTCGCCACCATCAGCGCGTCGTGGCTGCTCATCGCCGAGTGGGCAGGCCAGTCAACTTTCGGGTGCGGGGTGAAGAATTTATCCAGATTa                                                                    <  1:5763657/93‑1 (MQ=255)
                         cGCTCGCCACCATCAGCGCGTCGTGGCTGCTCATCGCCGAGTGGGCAGGCCAGTCAACTTTCGGGCGCGGGGTGAAGAATTTATCCAGATTAt                                                                   <  2:7110777/93‑1 (MQ=255)
                          nntCGCCACCATCAGCGCGTCGTGGCTGCTCATCGCCGAGTGGGCAGGCCAGTCAACTTTCGGGTGCGGGGTGAAGAATTTATCCAGATTATc                                                                  <  2:2084866/91‑1 (MQ=255)
                          gCTCGCCACCATCAGCGCGTCGTGGCTGCTCATCGCCGAGTGGGCAGGCCAGTCAACTTTCGGGTGCGGGGTGAAGAATTTATCCAGATTATc                                                                  <  1:494675/93‑1 (MQ=255)
                          gCTCGCCACCATCAGCGCGTCGTGGCTGCTCATCGCCGAGTGGGCAGGCCAGTCAACTTTCGGGCGCGGGGTGAAGAATTTATCCAGATTATc                                                                  <  1:5310808/93‑1 (MQ=255)
                           cTCGCCACCATCAGCGCGTCGTGGCTGCTCATCGCCGAGTGGGCAGGCCAGTCAACTTTCGGGTGCGGGGTGAAGAATTTATCCAGATTATCg                                                                 <  2:7250338/93‑1 (MQ=255)
                           cTCGCCACCATCAGCGCGTCGTGGCTGCTCATCGCCGAGTGGGCAGGCCAGTCAACTTTCGGGTGCGGGGTGAAGAATTTATCCAGATTATCg                                                                 <  2:4361218/93‑1 (MQ=255)
                           cTCGCCACCATCAGCGCGTCGTGGCTGCTCATCGCCGAGTGGGCAGGCCAGTCAACTTTCGGGTGCGGGGTGAAGAATTTATCCAGATTATCg                                                                 <  2:6820522/93‑1 (MQ=255)
                           cTCGCCACCATCAGCGCGTCGTGGCTGCTCATCGCCGAGTGGGCAGGCCAGTCAACTTTCGGGCGCGGGGTGAAGAATTTATCCAGATTATCg                                                                 <  2:6305507/93‑1 (MQ=255)
                           cTCGCCACCATCAGCGCGTCGTGGCTGCTCATCGCCGAGTGGGCAGGCCAGTCAACTTTCGGGCGCGGGGTGAAGAATTTATCCAGATTATCg                                                                 <  1:4281976/93‑1 (MQ=255)
                            tCGCCACCATCAGCGCGTCGTGGCTGCTCATCGCCGAGTGGGCAGGCCAGTCAACTTTCGGGTGCGGGGTGAAGAATTTATCCAGATTATCgg                                                                <  2:6722034/93‑1 (MQ=255)
                            tCGCCACCATCAGCGCGTCGTGGCTGCTCATCGCCGAGTGGGCAGGCCAGTCAACTTTCGGGTGCGGGGTGAAGAATTTATCCAGATTATCgg                                                                >  2:136242/1‑93 (MQ=255)
                             cGCCACCATCAGCGCGTCGTGGCTGCTCATCGCCGAGTGGGCAGGCCAGTCAACTTTCGGGTGCGGGGTGAAGAATTTATCCAGATTATCGGt                                                               <  1:7217767/93‑1 (MQ=255)
                               ccaccaTCAGCGCGTCGTGGCTGCTCATCGCCGAGTGGGCAGGCCAGTCAACTTTCGGGTGCGGGGTGAAGAATTTATCCAGATTATCGGTAt                                                             <  1:1477596/93‑1 (MQ=255)
                                caccaTCAGCGCGTCGTGGCTGCTCATCGCCGAGTGGGCAGGCCAGTCAACTTTCGGGTGCGGGGTGAAGAATTTATCCAGATTATCGGTATg                                                            >  1:7050228/1‑93 (MQ=255)
                                 accaTCAGCGCGTCGTGGCTGCTCATCGCCGAGTGGGCAGGCCAGTCAACTTTCGGGCGCGGGGTGAAGAATTTATCCAGATTATCGGTATGc                                                           >  1:7243945/1‑93 (MQ=255)
                                 accaTCAGCGCGTCGTGGCTGCTCATCGCCGAGTGGGCAGGCCAGTCAACTTTCGGGCGCGGGGTGAAGAATTTATCCAGATTATCGGTAAGc                                                           >  2:5265640/1‑93 (MQ=255)
                                  ccaTCAGCGCGTCGTGGCTGCTCATCGCCGAGTGGGCAGGCCAGTCAACTTTCGGGTGCGGGGTGAAGAATTTATCCAGATTATCGGTATGCa                                                          >  2:4757294/1‑93 (MQ=255)
                                  ccaTCAGCGCGTCGTGGCTGCTCATCGCCGAGTGGGCAGGCCAGTCAACTTTCGGGTGCGGGGTGAAGAATTTATCCAGATTATCGGTATGCa                                                          <  1:4409992/93‑1 (MQ=255)
                                  ccaTCAGCGCGTCGTGGCTGCTCATCGCCGAGTGGGCAGGCCAGTCAACTTTCGGGTGCGGGGTGAAGAATTTATCCAGATTATCGGTATGCa                                                          <  2:5398861/93‑1 (MQ=255)
                                    aTCAGCGCGTCGTGGCTGCTCATCGCCGAGTGGGCAGGCCAGTCAACTTTCGGGTGCGGGGTGAAGAATTTATCCAGATTATCGGTATGCAGc                                                        >  2:3608036/1‑93 (MQ=255)
                                    aTCAGCGCGTCGTGGCTGCTCATCGCCGAGTGGGCAGGCCAGTCAACTTTCGGGTGCGGGGTGAAGAATTTATCCAGATTATCGGTATGCAGc                                                        <  2:2713152/93‑1 (MQ=255)
                                     tCAGCGCGTCGTGGCTGCTCATCGCCGAGTGGGCAGGCCAGTCAACTTTCGGGTGCGGGGTGAAGAATTTATCCAGATTATCGGTATGCAGCt                                                       >  1:194709/1‑93 (MQ=255)
                                       aGCGCGTCGTGGCTGCTCATCGCCGAGTGGGCAGGCCAGTCAACTTTCGGGTGCGGGGTGAAGAATTTATCCAGATTATCGGTATGCAGCTCa                                                     <  2:4849855/93‑1 (MQ=255)
                                       aGCGCGTCGTGGCTGCTCATCGCCGAGTGGGCAGGCCAGTCAACTTTCGGGTGCGGGGTGAAGAATTTATCCAGATTATCGGTATGCAGCTCa                                                     >  2:1509324/1‑93 (MQ=255)
                                        gcgcGTCGTGGCTGCTCATCGCCGAGTGGGCAGGCCAGTCAACTTTCGGGTGCGGGGGGAAGAATTTATCCAGATTATCGGTATGCAGCTCaa                                                    >  1:74932/1‑93 (MQ=255)
                                         cgcgTCGTGGCTGCTCATCGCCGAGTGGGCAGGCCAGTCAACTTTCGGGTGCGGGGTGAAGAATTTATCCAGATTATCGGTATGCAGCTCAAt                                                   <  1:1504387/93‑1 (MQ=255)
                                          gcgTCGTGGCTGCTCATCGCCGAGTGGGCAGGCCAGTCAACTTTCGGGTGCGGGGTGAAGAATTTATCCAGATTATCGGTATGCAGCTCAATc                                                  <  2:7215587/93‑1 (MQ=255)
                                          gcgTCGTGGCTGCTCATCGCCGAGTGGGCAGGCCAGTCAACTTTCGGGCGCGGGGTGAAGAATTTATCCAGATTATCGGTATGCAGCTCAATc                                                  <  1:2987165/93‑1 (MQ=255)
                                           cgtcgtGGCTGCTCATCGCCGAGTGGGCAGGCCAGTCAACTTTCGGGTGCGGGGTGAAGAATTTATCCAGATTATCGGTATGCAGCTCAATCa                                                 <  2:1691720/93‑1 (MQ=255)
                                           cgtcgtGGCTGCTCATCGCCGAGTGGGCAGGCCAGTCAACTTTCGGGTGCGGGGTGAAGAATTTATCCAGATTATCGGTATGCAGCTCAATCa                                                 <  2:5978502/93‑1 (MQ=255)
                                           cgtcgtGGCTGCTCATCGCCGAGTGGGCAGGCCAGTCAACTTTCGGGCGCGGGGTGAAGAATTTATCCAGATTATCGGTATGCAGCTCAATCa                                                 >  2:1078461/1‑93 (MQ=255)
                                            gtcgtGGCTGCTCATCGCCGAGTGGGCAGGCCAGTCAACTTTCGGGTGCGGGGTGAAGAATTTATCCAGATTATCGGTATGCAGCTCAATCAg                                                <  2:6432303/93‑1 (MQ=255)
                                            gtcgtGGCTGCTCATCGCCGAGTGGGCAGGCCAGTCAACTTTCGGGTGCGGGGTGAAGAATTTATCCAGATTATCGGTATGCAGCTCAATCAg                                                >  1:5136807/1‑93 (MQ=255)
                                                    tgctCATCGCCGAGTGGGCAGGCCAGTCAACTTTCGGGTGCGGGGTGAAGAATTTATCCAGATTATCGGTATGCAGCTCAATCAGCCCCGgca                                        <  1:6043395/93‑1 (MQ=255)
                                                     gctCATCGCCGAGTGGGCAGGCCAGTCAACTTTCGGGTGCGGGGTGAAGAATTTATCCAGATTATCGGTATGCAGCTCAATCAGCCCCGgcag                                       <  1:5840572/93‑1 (MQ=255)
                                                          tCGCCGAGTGGGCAGGCCAGTCAACTTTCGGGTGCGGGGTGAAGAATTTATCCAGATTATCGGTATGCAGCTCAATCAGCCCCGGCAGcagcc                                  >  2:5146839/1‑93 (MQ=255)
                                                           cGCCGAGTGGGCAGGCCAGTCAACTTTCGGGTGCGGGGTGAAGAATTTATCCAGATTATCGGTATGCAGCTCAATCAGCCCCGGCAGcagcca                                 <  1:2673701/93‑1 (MQ=255)
                                                             ccGAGTGGGCAGGCCAGTCAACTTTCGGGTGCGGGGTGAAGAATTTATCCAGATTATCGGTATGCAGCTCAATCAGCCCCGGCAGcagccagc                               >  1:2229767/1‑93 (MQ=255)
                                                             ccGAGTGGGCAGGCCAGTCAACTTTCGGGCGCGGGGTGAAGAATTTATCCAGATTATCGGTATGCAGCTCAATCAGCCCCGGCAGcagccagc                               <  2:7440459/93‑1 (MQ=255)
                                                              cGAGTGGGCAGGCCAGTCAACTTTCGGGCGCGGGGTGAAGAATTTATCCAGATTATCGGTATGCAGCTCAATCAGCCCCGGCAGCagccagcc                              <  1:206663/93‑1 (MQ=255)
                                                                aGTGGGCAGGCCAGTCAACTTTCGGGTGCGGGGTGAAGAATTTATCCAGATTATCGGTATGCAGCTCAATCAGCCCCGGCAGCAGCCAgccgc                            <  1:1797106/93‑1 (MQ=255)
                                                                 gTGGGCAGGCCAGTCAACTTTCGGGTGCGGGGTGAAGAATTTATCCAGATTATCGGTATGCAGCTCAATCAGCCCCGGCAGCAGCCAgccgcc                           >  2:6335119/1‑93 (MQ=255)
                                                                  tGGGCAGGCCAGTCAACTTTCGGGTGCGGGGTGAAGAATTTATCCAGATTATCGGTATGCAGCTCAATCAGCCCCGGCAGCAGCCAGCCGCCt                          >  1:3838738/1‑93 (MQ=255)
                                                                     gCAGGCCAGTCAACTTTCGGGTGCGGGGTGAAGAATTTATCCAGATTATCGGTATGCAGCTCAATCAGCCCCGGCAGCAGCCAGCCGCCTTCg                       <  2:3305999/93‑1 (MQ=255)
                                                                       aGGCCAGTCAACTTTCGGGCGCGGGGTGAAGAATTTATCCAGATTATCGGTATGCAGCTCAATCAGCCCCGGCAGCAGCCAGCCGCCTTCGcc                     <  1:4454860/93‑1 (MQ=255)
                                                                        ggCCAGTCAACTTTCGGGTGCGGGGTGAAGAATTTATCCAGATTATCGGTATGCAGCTCAATCAGCCCCGGCAGCAGCCAGCCGCCTTCGCCg                    >  2:5723811/1‑93 (MQ=255)
                                                                         gCCAGTCAACTTTCGGGTGCGGGGTGAAGAATTTATCCAGATTATCGGTATGCAGCTCAATCAGCCCCGGCAGCAGCCAGCCGCCTTCGCCGt                   <  1:3636194/93‑1 (MQ=255)
                                                                            aGTCAACTTTCGGGTGCGGGGTGAAGAATTTATCCAGATTATCGGTATGCAGCTCAATCAGCCCCGGCAGCAGCCAGCCGCCTTCGCCGTCCa                <  1:2424671/93‑1 (MQ=255)
                                                                             gTCAACTTTCGGGTGCGGGGTGAAGAATTTATCCAGATTGTCGGTATGCAGCTCAATCAGCCCCGGCAGCAGCCAGCCGCCTTCGCCGTCCAt               <  1:81664/93‑1 (MQ=255)
                                                                             gTCAACTTTCGGGTGCGGGGTGAAGAATTTATCCAGATTATCGGTATGCAGCTCAATCAGCCCCGGCAGCAGCCAGCCGCCTTCGCCGTCCAt               <  1:4621252/93‑1 (MQ=255)
                                                                             gTCAACTTTCGGGTGCGGGGTGAAGAATTTATCCAGATTATCGGTATGCAGCTCAATCAGCCCCGGCAGCAGCCAGCCGCCTTCGCCGTCCAt               >  1:3188737/1‑93 (MQ=255)
                                                                                 aCTTTCGGGTGCGGGGTGAAGAATTTATCCAGATTATCGGTATGCAGCTCAATCAGCCCCGGCAGCAGCCAGCCGCCTTCGCCGTCCATCGcc           <  1:659189/93‑1 (MQ=255)
                                                                                  cTTTCGGGTGCGGGGTGAAGAATTTATCCAGATTATCGGTATGCAGCTCAATCAGCCCCGGCAGCAGCCAGCCGCCTTCGCCGTCCATCGCCt          <  2:42831/93‑1 (MQ=255)
                                                                                   tttCGGGTGCGGGGTGAAGAATTTATCCAGATTATCGGTATGCAGCTCAATCAGCCCCGGCAGCAGCCAGCCGCCTTCGCCGTCCATCGCCTc         <  1:5045680/93‑1 (MQ=255)
                                                                                   tttCGGGTGCGGGGTGAAGAATTTATCCAGATTATCGGTATGCAGCTCAATCAGCCCCGGCAGCAGCCAGCCGCCTTCGCCGTCCATCGCCTc         <  2:2048875/93‑1 (MQ=255)
                                                                                    ttCGGGTGCGGGGTGAAGAATTTATCCAGATTATCGGTATGCAGCTCAATCAGCCCCGGCAGCAGCCAGCCGCCTTCGCCGTCCATCGCCTcc        <  2:2260364/93‑1 (MQ=255)
                                                                                    ttCGGGTGCGGGGTGAAGAATTTATCCAGATTATCGGTATGCAGCTCAATCAGCCCCGGCAGCAGCCAGCCGCCTTCGCCGTCCATCGCCTcc        >  1:5601027/1‑93 (MQ=255)
                                                                                    ttCGGGTGCGGGGTGAAGAATTTATCCAGATTATCGGTATGCAGCTCAATCAGCCCCGGCAGCAGCCAGCCGCCTTCGCCGTCCATCGCCTcc        >  2:2929532/1‑93 (MQ=255)
                                                                                        ggCGCGGGGTGAAGAATTTATCCAGATTATCGGTATGCAGCTCAATCAGCCCCGGCAGCAGCCAGCCGCCTTCGCCGTCCATCGCCTCCGGCa    <  2:4012987/93‑1 (MQ=255)
                                                                                         gcgcGGGGTGAAGAATTTATCCAGATTATCGGTATGCAGCTCAATCAGCCCCGGCAGCAGCCAGCCGCCTTCGCCGTCCATCGCCTCCGGCAg   <  1:5192952/93‑1 (MQ=255)
                                                                                          cgcgGGGTGAAGAATTTATCCAGATTATCGGTATGCAGCTCAATCAGCCCCGGCAGCAGCCAGCCGCCTTCGCCGTCCATCGCCTCCGGCAgg  <  1:6291397/93‑1 (MQ=255)
                                                                                          |                                                                                            
ACGGCATCCAGTACGGTGGTGATGCCGCTCGCCACCATCAGCGCGTCGTGGCTGCTCATCGCCGAGTGGGCAGGCCAGTCAACTTTCGGGCGCGGGGTGAAGAATTTATCCAGATTATCGGTATGCAGCTCAATCAGCCCCGGCAGCAGCCAGCCGCCTTCGCCGTCCATCGCCTCCGGCAGG  >  NC_000913/4314510‑4314692

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CCACGGCATCCAGTACGGTGGTGATGCCGCTCGCCACCATCAGCGCGTCGTGGCTGCTCATCGCCGAGTGGGCAGGCCAGTCAACTTTCGGGCGCGGGGTGAAGAATTTATCCAGATTATCGGTATGCAGCTCAATCAGCCCCGGCAGCAGCCAGCCGCCTTCGCCGTCCATCGCCTCCGGCAGGC  >  NC_000913/4314508‑4314693
                                                                                            |                                                                                             
CCACGGCATCCAGTACGGTGGTGATGCCGCTCGCCACCATCAGCGCGTCGTGGCTGCTCATCGCCGAGTGGGCAGGCCAGTCAACTTTCGGGTG                                                                                              <  SRR4302542.3108999/94‑1 (MQ=60)
CCACGGCATCCAGTACGGTGGTGATGCCGCTCGCCACCATCAGCGCGTCGTGGCTGCTCATCGCCGAGTGGGCAGGCCAGTCAACTTTCGGGTG                                                                                              <  SRR4302542.5434750/94‑1 (MQ=60)
 CACGGCATCCAGTACGGTGGTGATGCCGCTCGCCACCATCAGCGCGTCGTGGCTGCTCATCGCCGAGTGGGCAGGCCAGTCAACTTTCGGGTGC                                                                                             <  SRR4302542.1597139/94‑1 (MQ=60)
 CACGGCATCCAGTACGGTGGTGATGCCGCTCGCCACCATCAGCGCGTCGTGGCTGCTCATCGCCGAGTGGGCAGGCCAGTCAACTTTCGGGTGC                                                                                             >  SRR4302542.557812/1‑94 (MQ=60)
  ACGGCATCCAGTACGGTGGTGATGCCGCTCGCCACCATCAGCGCGTCGTGGCTGCTCATCGCCGAGTGGGCAGGCCAGTCAACTTTCGGGTGCG                                                                                            >  SRR4302542.4244134/1‑94 (MQ=60)
  ACGGCATCCAGTACGGTGGTGATGCCGCTCGCCACCATCAGCGCGTCGTGGCTGCTCATCGCCGAGTGGGCAGGCCAGTCAACTTTCGGGTGCG                                                                                            >  SRR4302542.3934727/1‑94 (MQ=60)
   CGGCATCCAGTACGGTGGTGATGCCGCTCGCCACCATCAGCGCGTCGTGGCTGCTCATCGCCGAGTGGGCAGGCCAGTCAACTTTCGGGTGCGG                                                                                           >  SRR4302542.4558379/1‑94 (MQ=60)
   CGGCATCCAGTACGGTGGTGATGCCGCTCGCCACCATCAGCGCGTCGTGGCTGCTCATCGCCGAGTGGGCAGGCCAGTCAACTTTCGGGCGCGG                                                                                           >  SRR4302542.5475865/1‑94 (MQ=60)
    GGCATCCAGTACGGTGGTGATGCCGCTCGCCACCATCAGCGCGTCGTGGCTGCTCATCGCCGAGTGGGCAGGCCAGTCAACTTTCGGGTGCGGG                                                                                          <  SRR4302542.2827473/94‑1 (MQ=60)
     GCATCCAGTACGGTGGTGATGCCGCTCGCCACCATCAGCGCGTCGTTGCTGCTCATCGCCGAGTGGGCAGGCCAGTCAACTTTCGGGCGCGGGG                                                                                         <  SRR4302542.1300769/94‑1 (MQ=60)
     GCATCCAGTACGGTGGTGATGCCGCTCGCCACCATCAGCGCGTCGTGGCTGCTCATCGCCGAGTGGGCAGGCCAGTCAACTTTCGGGTGCGGGG                                                                                         <  SRR4302542.714101/94‑1 (MQ=60)
     GCATCCAGTACGGTGGTGATGCCGCTCGCCACCATCAGCGCGTCGTGGCTGCTCATCGCCGAGTGGGCAGGCCAGTCAACTTTCGGGTGCGGGG                                                                                         <  SRR4302542.7305875/94‑1 (MQ=60)
     GCATCCAGTACGGTGGTGATGCCGCTCGCCACCATCAGCGCGTCGTGGCTGCTCATCGCCGAGTGGGCAGGCCAGTCAACTTTCGGGTGCGGGG                                                                                         <  SRR4302542.2727680/94‑1 (MQ=60)
     GCATCCAGTACGGTGGTGATGCCGCTCGCCACCATCAGCGCGTCGTGGCTGCTCATCGCCGAGTGGGCAGGCCAGTCAACTTTCGGGTGCGGGG                                                                                         <  SRR4302542.2543726/94‑1 (MQ=60)
     GCATCCAGTACGGTGGTGATGCCGCTCGCCACCATCAGCGCGTCGTGGCTGCTCATCGCCGAGTGGGCAGGCCAGTCAACTTTCGGGCGCGGGG                                                                                         <  SRR4302542.1640876/94‑1 (MQ=60)
       ATCCAGTACGGTGGTGATGCCGCTCGCCACCATCAGCGCGTCGTGGCTGCTCATCGCCGAGTGGGCAGGCCAGTCAACTTTCGGGTGCGGGGTG                                                                                       <  SRR4302542.4985153/94‑1 (MQ=60)
       ATCCAGTACGGTGGTGATGCCGCTCGCCACCATCAGCGCGTCGTGGCTGCTCATCGCCGAGTGGGCAGGCCAGTCAACTTTCGGGTGCGGGGTG                                                                                       >  SRR4302542.1141114/1‑94 (MQ=60)
       ATCCAGTACGGTGGTGATGCCGCTCGCCACCATCAGCGCGTCGTGGCTGCTCATCGCCGAGTGGGCAGGCCAGTCAACTTTCGGGTGCGGGGGG                                                                                       >  SRR4302542.6279978/1‑94 (MQ=60)
         CCAGTACGGTGGTGATGCCGCTCGCCACCATCAGCGCGTCGTGGCTGCTCATCGCCGAGTGGGCAGGCCAGTCAACTTTCGGGTGCGGGGCGAA                                                                                     >  SRR4302542.4935370/1‑94 (MQ=60)
          CAGTACGGTGGTGATGCCGCTCGCCACCATCAGCGCGTCGTGGCTGCTCATCGCCGAGTGGGCAGGCCAGTCAACTTTCGGGTGCGGGGTGAAG                                                                                    <  SRR4302542.4891050/94‑1 (MQ=60)
          CAGTACGGTGGTGATGCCGCTCGCCACCATCAGCGCGTCGTGGCTGCTCATCGCCGAGTGGGCAGGCCAGTCAACTTTCGGGTGCGGGGTGAAG                                                                                    <  SRR4302542.2676023/94‑1 (MQ=60)
          CAGTACGGTGGTGATGCCGCTCGCCACCATCAGCGCGTCGTGGCTGCTCATCGCCGAGTGGGCAGGCCAGTCAACTTTCGGGTGCGGGGCGAAG                                                                                    >  SRR4302542.6459782/1‑94 (MQ=60)
            GTACGGTGGTGATGCCGCTCGCCACCATCAGCGCGTCGTGGCTGCTCATCGCCGAGTGGGCAGGCCAGTCAACTTTCGGGTGCGGGGTGAAGAA                                                                                  >  SRR4302542.1918693/1‑94 (MQ=60)
            GTACGGTGGTGATGCCGCTCGCCACCATCAGCGCGTCGTGGCTGCTCATCGCCGAGTGGGCAGGCCAGTCAACTTTCGGGTGCGGGGTGAAGAA                                                                                  <  SRR4302542.2979714/94‑1 (MQ=60)
            GTACGGTGGTGATGCCGCTCGCCACCATCAGCGCGTCGTGGCTGCTCATCGCCGAGTGGGCAGGCCAGTCAACTTTCGGGTGCGGGGTGAAGAA                                                                                  <  SRR4302542.6859393/94‑1 (MQ=60)
             TACGGTGGTGATGCCGCTCGCCACCATCAGCGCGTCGTGGCTGCTCATCGCCGAGTGGGCAGGCCAGTCAACTTTCGGGTGCGGGGTGAAGAAT                                                                                 <  SRR4302542.3049370/94‑1 (MQ=60)
             TACGGTGGTGATGCCGCTCGCCACCATCAGCGCGTCGTGGCTGCTCATCGCCGAGTGGGCAGGCCAGTCAACTTTCGGGTGCGGGGTGAAGAAT                                                                                 >  SRR4302542.1820810/1‑94 (MQ=60)
              ACGGTGGTGATGCCGCTCGCCACCATCAGCGCGTCGTGGCTGCTCATCGCCGAGTGGGCAGGCCAGTCAACTTTCGGGTGCGGGGTGAAGAATT                                                                                <  SRR4302542.4660990/94‑1 (MQ=60)
                GGTGGTGATGCCGCTCGCCACCATCAGCGCGTCGTGGCTGCTCATCGCCGAGTGGGCAGGCCAGTCAACTTTCGGGTGCGGGGTGAAGAATTTA                                                                              >  SRR4302542.5566013/1‑94 (MQ=60)
                GGTGGTGATGCCGCTCGCCACCATCAGCGCGTCGTGGCTGCTCATCGCCGAGTGGGCAGGCCAGTCAACTTTCGGGCGCGGGGTGAAGAATTTA                                                                              <  SRR4302542.6667161/94‑1 (MQ=60)
                GGTGGTGATGCCGCTCGCCACCATCAGCGCGTCGTGGCTGCTCATCGCCGAGTGGGCAGGCCAGTCAACTTTCGGGCGCGGGGGGAAGAATTTA                                                                              >  SRR4302542.6072698/1‑94 (MQ=60)
                 GTGGTGATGCCGCTCGCCACCATCAGCGCGTCGTGGCTGCTCATCGCCGAGTGGGCAGGCCAGTCAACTTTCGGGTGCGGGGTGAAGAATTTAT                                                                             <  SRR4302542.5608668/94‑1 (MQ=60)
                 GTGGTGATGCCGCTCGCCACCATCAGCGCGTCGTGGCTGCTCATCGCCGAGTGGGCAGGCCAGTCAACTTTCGGGTGCGGGGTGAAGAATTTAT                                                                             >  SRR4302542.6288989/1‑94 (MQ=60)
                   GGTGATGCCGCTCGCCACCATCAGCGCGTCGTGGCTGCTCATCGCCGAGTGGGCAGGCCAGTCAACTTTCGGGTGCGGGGTGAAGAATTTATCC                                                                           <  SRR4302542.5269118/94‑1 (MQ=60)
                   GGTGATGCCGCTCGCCACCATCAGCGCGTCGTGGCTGCTCATCGCCGAGTGGGCAGGCCAGTCAACTTTCGGGTGCGGGGTGAAGAATTTATCC                                                                           <  SRR4302542.2447974/94‑1 (MQ=60)
                   GGTGATGCCGCTCGCCACCATCAGCGCGTCGTGGCTGCTCATCGCCGAGTGGGCAGGCCAGTCAACTTTCGGGTGCGGGGTGAAGAATTTATCC                                                                           <  SRR4302542.1425474/94‑1 (MQ=60)
                    GTGATGCCGCTCGCCACCATCAGCGCGTCGTGGCTGCTCATCGCCGAGTGGGCAGGCCAGTCAACTTTCGGGTGCGGGGTGAAGAATTTATCCA                                                                          <  SRR4302542.285555/94‑1 (MQ=60)
                     TGATGCCGCTCGCCACCATCAGCGCGTCGTGGCTGCTCATCGCCGAGTGGGCAGGCCAGTCAACTTTCGGGTGCGGGGTGAAGAATTTATCCAG                                                                         <  SRR4302542.5115788/94‑1 (MQ=60)
                     TGATGCCGCTCGCCACCATCAGCGCGTCGTGGCTGCTCATCGCCGAGTGGGCAGGCCAGTCAACTTTCGGGTGCGGGGTGAAGAATTTATCCAG                                                                         <  SRR4302542.5771232/94‑1 (MQ=60)
                       ATGCCGCTCGCCACCATCAGCGCGTCGTGGCTGCTCATCGCCGAGTGGGCAGGCCAGTCAACTTTCGGGTGCGGGGTGAAGAATTTATCCAGAT                                                                       <  SRR4302542.5171277/94‑1 (MQ=60)
                       ATGCCGCTCGCCACCATCAGCGCGTCGTGGCTGCTCATCGCCGAGTGGGCAGGCCAGTCAACTTTCGGGTGCGGGGTGAAGAATTTATCCAGAT                                                                       <  SRR4302542.173964/94‑1 (MQ=60)
                          CCGCTCGCCACCATCAGCGCGTCGTGGCTGCTCATCGCCGAGTGGGCAGGCCAGTCAACTTTCGGGTGCGGGGTGAAGAATTTATCCAGATTAT                                                                    <  SRR4302542.5819601/94‑1 (MQ=60)
                            GCTCGCCACCATCAGCGCGTCGTGGCTGCTCATCGCCGAGTGGGCAGGCCAGTCAACTTTCGGGTGCGGGGTGAAGAATTTATCCAGATTATCG                                                                  <  SRR4302542.1813350/94‑1 (MQ=60)
                            GCTCGCCACCATCAGCGCGTCGTGGCTGCTCATCGCCGAGTGGGCAGGCCAGTCAACTTTCGGGTGCGGGGTGAAGAATTTATCCAGATTATCG                                                                  <  SRR4302542.498937/94‑1 (MQ=60)
                            GCTCGCCACCATCAGCGCGTCGTGGCTGCTCATCGCCGAGTGGGCAGGCCAGTCAACTTTCGGGCGCGGGGTGAAGAATTTATCCAGATTATCG                                                                  <  SRR4302542.5362403/94‑1 (MQ=60)
                             CTCGCCACCATCAGCGCGTCGTGGCTGCTCATCGCCGAGTGGGCAGGCCAGTCAACTTTCGGGCGCGGGGTGAAGAATTTATCCAGATTATCGG                                                                 <  SRR4302542.4324097/94‑1 (MQ=60)
                               CGCCACCATCAGCGCGTCGTGGCTGCTCATCGCCGAGTGGGCAGGCCAGTCAACTTTCGGGTGCGGGGTGAAGAATTTATCCAGATTATCGGTA                                                               <  SRR4302542.7288573/94‑1 (MQ=60)
                                GCCACCATCAGCGCGTCGTGGCTGCTCCTCGCCGAGAGGGCAGGCCAGTCAACTTTCGGGTGCGGGGGGAAGAATTTATCTAGATTtttgttat                                                              >  SRR4302542.7522502/1‑86 (MQ=60)
                                GCCACCATCAGCGCGTCGTGGCTGCTCATCGCCGAGTGGGCAGGCCAGTCAACTTTCGGGTGCGGGGTGAAGAATTTATCCAGATTATCGGTAT                                                              <  SRR4302542.4403576/94‑1 (MQ=60)
                                GCCACCATCAGCGCGTCGTGGCTGCTCATCGCCGAGTGGGCAGGCCAGTCAACTTTCGGGTGCGGGGTGAAGAATTTATCCAGATTATCGGTAT                                                              <  SRR4302542.4902692/94‑1 (MQ=60)
                                GCCACCATCAGCGCGTCGTGGCTGCTCATCGCCGAGTGGGCAGGCCAGTCAACTTTCGGGTGCGGGGTGAAGAATTTATCCAGATTATCGGTAT                                                              <  SRR4302542.2623921/94‑1 (MQ=60)
                                GCCACCATCAGCGCGTCGTGGCTGCTCATCGCCGAGTGGGCAGGCCAGTCAACTTTCGGGTGCGGGGTGAAGAATTTATCCAGATTATCGGTAT                                                              <  SRR4302542.5100518/94‑1 (MQ=60)
                                GCCACCATCAGCGCGTCGTGGCTGCTCATCGCCGAGTGGGCAGGCCAGTCAACTTTCGGGTGCGGGGTGAAGAATTTATCCAGATTATCGGTAT                                                              >  SRR4302542.6153775/1‑94 (MQ=60)
                                 CCACCATCAGCGCGTCGTGGCTGCTCATCGCCGAGTGGGCAGGCCAGTCAACTTTCGGGTGCGGGGTGAAGAATTTATCCAGATTATCGGTATG                                                             >  SRR4302542.7119444/1‑94 (MQ=60)
                                 CCACCATCAGCGCGTCGTGGCTGCTCATCGCCGAGTGGGCAGGCCAGTCAACTTTCGGGTGCGGGGTGAAGAATTTATCCAGATTATCGGTATG                                                             <  SRR4302542.1490342/94‑1 (MQ=60)
                                 CCACCATCAGCGCGTCGTGGCTGCTCATCGCCGAGTGGGCAGGCCAGTCAACTTTCGGGTGCGGGGCGAAGAATTTATCCAGATTATCGGTATG                                                             >  SRR4302542.7209377/1‑94 (MQ=60)
                                  CACCATCAGCGCGTCGTGGCTGCTCATCGCCGAGTGGGCAGGCCAGTCAACTTTCGGGCGCGGGGTGAAGAATTTATCCAGATTATCGGTATGC                                                            >  SRR4302542.7314981/1‑94 (MQ=60)
                                   ACCATCAGCGCGTCGTGGCTGCTCATCGCCGAGTGGGCAGGCCAGTCAACTTTCGGGTGCGGGGTGAAGAATTTATCCAGATTATCGGTATGCA                                                           >  SRR4302542.5086661/1‑94 (MQ=60)
                                    CCATCAGCGCGTCGTGGCTGCTCATCGCCGAGTGGGCAGGCCAGTCAACTTTCGGGTGCGGGGTGAAGAATTTATCCAGATTATCGGTATGCAG                                                          <  SRR4302542.762653/94‑1 (MQ=60)
                                    CCATCAGCGCGTCGTGGCTGCTCATCGCCGAGTGGGCAGGCCAGTCAACTTTCGGGTGCGGGGTGAAGAATTTATCCAGATTATCGGTATGCAG                                                          <  SRR4302542.4453301/94‑1 (MQ=60)
                                     CATCAGCGCGTCGTGGCTGCTCATCGCCGAGTGGGCAGGCCAGTCAACTTTCGGGTGCGGGGTGAAGAATTTATCCAGATTATCGGTATGCAGC                                                         >  SRR4302542.705822/1‑94 (MQ=60)
                                      ATCAGCGCGTCGTGGCTGCTCATCGCCGAGTGGGCAGGCCAGTCAACTTTCGGGTGCGGGGTGAAGAATTTATCCAGATTATCGGTATGCAGCT                                                        >  SRR4302542.1506896/1‑94 (MQ=60)
                                      ATCAGCGCGTCGTGGCTGCTCATCGCCGAGTGGGCAGGCCAGTCAACTTTCGGGTGCGGGGTGAAGAATTTATCCAGATTATCGGTATGCAGCT                                                        >  SRR4302542.196361/1‑94 (MQ=60)
                                         AGCGCGTCGTGGCTGCTCATCGCCGAGTGGGCAGGCCAGTCAACTTTCGGGTGCGGGGTGAAGAATTTATCCAGATTATCGGTATGCAGCTCAA                                                     <  SRR4302542.517118/94‑1 (MQ=60)
                                         AGCGCGTCGTGGCTGCTCATCGCCGAGTGGGCAGGCCAGTCAACTTTCGGGTGCGGGGGGAAGAATTTATCCAGATTATCGGTATGCAGCTCAA                                                     >  SRR4302542.75553/1‑94 (MQ=60)
                                           CGCGTCGTGGCTGCTCATCGCCGAGTGGGCAGGCCAGTCAACTTTCGGGTGCGGGGTGAAGAATTTATCCAGATTATCGGTATGCAGCTCAATC                                                   <  SRR4302542.1851787/94‑1 (MQ=60)
                                           CGCGTCGTGGCTGCTCATCGCCGAGTGGGCAGGCCAGTCAACTTTCGGGTGCGGGGTGAAGAATTTATCCAGATTATCGGTATGCAGCTCAATC                                                   <  SRR4302542.1517400/94‑1 (MQ=60)
                                            GCGTCGTGGCTGCTCATCGCCGAGTGGGCAGGCCAGTCAACTTTCGGGTGCGGGGTGAAGAATTTATCCAGATTATCGGTATGCAGCTCAATCA                                                  <  SRR4302542.5193333/94‑1 (MQ=60)
                                            GCGTCGTGGCTGCTCATCGCCGAGTGGGCAGGCCAGTCAACTTTCGGGCGCGGGGTGAAGAATTTATCCAGATTATCGGTATGCAGCTCAATCA                                                  <  SRR4302542.3015761/94‑1 (MQ=60)
                                             CGTCGTGGCTGCTCATCGCCGAGTGGGCAGGCCAGTCAACTTTCGGGTGCGGGGTGAAGAATTTATCCAGATTATCGGTATGCAGCTCAATCAG                                                 >  SRR4302542.5186734/1‑94 (MQ=60)
                                             CGTCGTGGCTGCTCATCGCCGAGTGGGCAGGCCAGTCAACTTTCGGGTGCGGGGTGAAGAATTTATCCAGATTATCGGTATGCAGCTCAATCAG                                                 <  SRR4302542.1294505/94‑1 (MQ=60)
                                             CGTCGTGGCTGCTCATCGCCGAGTGGGCAGGCCAGTCAACTTTCGGGTGCGGGGTGAAGAATTTATCCAGATTATCGGTATGCAGCTCAATCAG                                                 >  SRR4302542.6306158/1‑94 (MQ=60)
                                             CGTCGTGGCTGCTCATCGCCGAGTGGGCAGGCCAGTCAACTTTCGGGTGCGGGGTGAAGAATTTATCCAGATTATCGGTATGCAGCTCAATCAG                                                 >  SRR4302542.2154561/1‑94 (MQ=60)
                                             CGTCGTGGCTGCTCATCGCCGAGTGGGCAGGCCAGTCAACTTTCGGGTGCGGGGTGAAGAATTTATCCAGATTATCGGTATGCAGCTCAATCAG                                                 <  SRR4302542.3115741/94‑1 (MQ=60)
                                              GTCGTGGCTGCTCATCGCCGAGTGGGCAGGCCAGTCAACTTTCGGGTGCGGGGTGAAGAATTTATCCAGATTATCGGTATGCAGCTCAATCAGC                                                >  SRR4302542.4494270/1‑94 (MQ=60)
                                              GTCGTGGCTGCTCATCGCCGAGTGGGCAGGCCAGTCAACTTTCGGGTGCGGGGTGAAGAATTTATCCAGATTATCGGTATGCAGCTCAATCAGC                                                >  SRR4302542.3941166/1‑94 (MQ=60)
                                               TCGTGGCTGCTCATCGCCGAGTGGGCAGGCCAGTCAACTTTCGGGTGCGGGGTGAAGAATTTATCCAGATTATCGGTATGCAGCTCAATCAGCC                                               <  SRR4302542.7388546/94‑1 (MQ=60)
                                                CGTGGCTGCTCATCGCCGAGTGGGCAGGCGAGTCACCTTTCGGGTGCGGGGTGAAAAATTTATCTAGATTATCGGTATGCAGCTCAATCAGCCC                                              <  SRR4302542.654398/94‑1 (MQ=60)
                                                   GGCTGCTCATCGCCGAGTGGGCAGGCCAGTTAACTTTCGGGTGCGGGGTGAAGAATTTATCCAGATTATCGGTATGCAGCTCAATCAGCCCCGG                                           <  SRR4302542.4206092/94‑1 (MQ=60)
                                                   GGCTGCTCATCGCCGAGTGGGCAGGCCAGTCAACTTTCGGGTGCGGGGTGAAGAATTTATCCAGATTATCGGTATGCAGCTCAATCAGCCCCGG                                           <  SRR4302542.498085/94‑1 (MQ=60)
                                                   GGCTGCTCATCGCCGAGTGGGCAGGCCAGTCAACTTTCGGGTGCGGGGTGAAGAATTTATCCAGATTATCGGTATGCAGCTCAATCAGCCCCGG                                           <  SRR4302542.3901965/94‑1 (MQ=60)
                                                      TGCTCCTCGCCGAGTGGGCAGGCCAGTCAACTTTCGGGTGCGGGGTGAAGAATTTATCCAGATTATCGGTATGCAGCTCAATCAGCCCCGGCAG                                        >  SRR4302542.530177/1‑94 (MQ=60)
                                                      TGCTCATCGCCGAGTGGGCAGGCCAGTCAACTTTCGGGTGCGGGGTGAAGAATTTATCCAGATTATCGGTATGCAGCTCAATCAGCCCCGGCAG                                        <  SRR4302542.6101892/94‑1 (MQ=60)
                                                       GCTCATCGCCGAGTGGGCAGGCCAGTCAACTTTCGGGTGCGGGGTGAAGAATTTATCCAGATTATCGGTATGCAGCTCAATCAGCCCCGGCAGC                                       <  SRR4302542.5897232/94‑1 (MQ=60)
                                                       GCTCATCGCCGAGTGGGCAGGCCAGTCAACTTTCGGGTGCGGGGTGAAGAATTTATCCAGATTATCGGTATGCAGCTCAATCAGCCCCGGCAGC                                       >  SRR4302542.3550297/1‑94 (MQ=60)
                                                           ATCGCCGAGTGGGCAGGCCAGTCAACTTTCGGGTGCGGGGTGAAGAATTTATCCAGATTATCGGTATGCAGCTCAATCAGCCCCGGCAGCAGCC                                   >  SRR4302542.5044230/1‑94 (MQ=60)
                                                            TCGCCGAGTGGGCAGGCCAGTCAACTTTCGGGCGCGGGGTGAAGAATTTATCCAGATTATCGGTATGCAGCTCAATCAGCCCCGGCAGCAGCCA                                  <  SRR4302542.1428450/94‑1 (MQ=60)
                                                             CGCCGAGTGGGCAGGCCAGTCAACTTTCGGGTGCGGGGTGAAGAATTTATCCAGATTATCGGTATGCAGCTCAATCAGCCCCGGCAGCAGCCAG                                 <  SRR4302542.4333184/94‑1 (MQ=60)
                                                             CGCCGAGTGGGCAGGCCAGTCAACTTTCGGGTGCGGGGTGAAGAATTTATCCAGATTATCGGTATGCAGCTCAATCAGCCCCGGCAGCAGCCAG                                 <  SRR4302542.2699505/94‑1 (MQ=60)
                                                              GCCGAGTGGGCAGGCCAGTCAACTTTCGGGTGCGGGGTGAAGAATTTATCCAGATTATCGGTATGCAGCTCAATCAGCCCCGGCAGCAGCCAGC                                >  SRR4302542.2248953/1‑94 (MQ=60)
                                                                CGAGTGGGCAGGCCAGTCAACTTTCGGGTGCGGGGTGAAGAATTTATCCAGATTATCGGTATGCAGCTCAATCAGCCCCGGCAGCAGCCAGCCG                              <  SRR4302542.7426128/94‑1 (MQ=60)
                                                                CGAGTGGGCAGGCCAGTCAACTTTCGGGCGCGGGGTGAAGAATTTATCCAGATTATCGGTATGCAGCTCAATCAGCCCCGGCAGCAGCCAGCCG                              <  SRR4302542.208450/94‑1 (MQ=60)
                                                                CGAGTGGGCAGGCCAGTCAACTTTCGGGCGCGGGGTGAAGAATTTATCCAGATTATCGGTATGCAGCTCAATCAGCCCCGGCAGCAGCCAGCCG                              <  SRR4302542.2069611/94‑1 (MQ=60)
                                                                 GAGTGGGCAGGCCAGTCAACTTTCGGGTGCGGGGTGAAGAATTTATCCAGATTATCGGTATGCAGCTCAATCAGCCCCGGCAGCAGCCAGCCGC                             >  SRR4302542.5396378/1‑94 (MQ=60)
                                                                 GAGTGGGCAGGCCAGTCAACTTTCGGGTGCGGGGTGAAGAATTTATCCAGATTATCGGTATGCAGCTCAATCAGCCCCGGCAGCAGCCAGCCGC                             >  SRR4302542.19259/1‑94 (MQ=60)
                                                                  AGTGGGCAGGCCAGTCAACTTTCGGGTGCGGGGTGAAGAATTTATCCAGATTATCGGTATGCAGCTCAATCAGCCCCGGCAGCAGCCAGCCGCC                            <  SRR4302542.1812693/94‑1 (MQ=60)
                                                                   GTGGGCAGGCCAGTCAACTTTCGGGTGCGGGGTGAAGAATTTATCCAGATTATCGGTATGCAGCTCAATCAGCCCCGGCAGCAGCCAGCCGCCT                           >  SRR4302542.3876698/1‑94 (MQ=60)
                                                                     GGGCAGGCCAGTCAACTTTCGGGCGCGGGGTGAAGAATTTATCCAGATTATCGGTATGCAGCTCAATCAGCCCCGGCAGCAGCCAGCCGCCTTC                         >  SRR4302542.6682752/1‑94 (MQ=60)
                                                                         AGGCCAGTCAACTTTCGGGTGCGGGGTGAAGAATTTATCCAGATTATCGGTATGCAGCTCAATCAGCCCCGGCAGCAGCCAGCCGCCTTCGCCG                     >  SRR4302542.2068275/1‑94 (MQ=60)
                                                                         AGGCCAGTCAACTTTCGGGCGCGGGGTGAAGAATTTATCCAGATTATCGGTATGCAGCTCAATCAGCCCCGGCAGCAGCCAGCCGCCTTCGCCG                     <  SRR4302542.4498562/94‑1 (MQ=60)
                                                                           GCCAGTCAACTTTCGGGTGCGGGGTGAAGAATTTATCCAGATTATCGGTATGCAGCTCAATCAGCCCCGGCAGCAGCCAGCCGCCTTCGCCGTC                   >  SRR4302542.3868712/1‑94 (MQ=60)
                                                                           GCCAGTCAACTTTCGGGTGCGGGGTGAAGAATTTATCCAGATTATCGGTATGCAGCTCAATCAGCCCCGGCAGCAGCCAGCCGCCTTCGCCGTC                   <  SRR4302542.3670103/94‑1 (MQ=60)
                                                                             CAGTCAACTTTCGGGTGCGGGGTGAAGAATTTATCCAGATTATCGGTATGCAGCTCAATCAGCCCCGGCAGCAGCCAGCCGCCTTCGCCGTCCA                 <  SRR4302542.5874399/94‑1 (MQ=60)
                                                                             CAGTCAACTTTCGGGTGCGGGGTGAAGAATTTATCCAGATTATCGGTATGCAGCTCAATCAGCCCCGGCAGCAGCCAGCCGCCTTCGCCGTCCA                 >  SRR4302542.2823511/1‑94 (MQ=60)
                                                                             CAGTCAACTTTCGGGTGCGGGGTGAAGAATTTATCCAGATTATCGGTATGCAGCTCAATCAGCCCCGGCAGCAGCCAGCCGCCTTCGCCGTCCA                 <  SRR4302542.2287732/94‑1 (MQ=60)
                                                                             CAGTCAACTTTCGGGTGCGGGGTGAAGAATTTATCCAGATTATCGGTATGCAGCTCAATCAGCCCCGGCAGCAGCCAGCCGCCTTCGCCGTCCA                 >  SRR4302542.3831881/1‑94 (MQ=60)
                                                                              AGTCAACTTTCGGGTGCGGGGTGAAGAATTTATCCAGATTATCGGTATGCAGCTCAATCAGCCCCGGCAGCAGCCAGCCGCCTTCGCCGTCCAT                >  SRR4302542.3218940/1‑94 (MQ=60)
                                                                              AGTCAACTTTCGGGTGCGGGGTGAAGAATTTATCCAGATTATCGGTATGCAGCTCAATCAGCCCCGGCAGCAGCCAGCCGCCTTCGCCGTCCAT                >  SRR4302542.1836398/1‑94 (MQ=60)
                                                                              AGTCAACTTTCGGGTGCGGGGTGAAGAATTTATCCAGATTATCGGTATGCAGCTCAATCAGCCCCGGCAGCAGCCAGCCGCCTTCGCCGTCCAT                <  SRR4302542.2448256/94‑1 (MQ=60)
                                                                              AGTCAACTTTCGGGTGCGGGGTGAAAAATTTATCCAGATTATCGGTATGCAGCTCAATCAGCCCCGGCAGCAGCCAGCCGCCTTCGCCGTCCAT                <  SRR4302542.4244154/94‑1 (MQ=60)
                                                                               GTCAACTTTCGGGTGCGGGGTGAAGAATTTATCCAGATTGTCGGTATGCAGCTCAATCAGCCCCGGCAGCAGCCAGCCGCCTTCGCCGTCCATC               <  SRR4302542.82347/94‑1 (MQ=60)
                                                                               GTCAACTTTCGGGTGCGGGGTGAAGAATTTATCCAGATTATCGGTATGCAGCTCAATCAGCCCCGGCAGCAGCCAGCCGCCTTCGCCGTCCATC               <  SRR4302542.4666400/94‑1 (MQ=60)
                                                                               GTCAACTTTCGGGTGCGGGGTGAAGAATTTATCCAGATTATCGGTATGCAGCTCAATCAGCCCCGGCAGCAGCCAGCCGCCTTCGCCGTCCATC               <  SRR4302542.6393452/94‑1 (MQ=60)
                                                                               GTCAACTTTCGGGTGCGGGGTGAAGAATTTATCCAGATTATCGGTATGCAGCTCAATCAGCCCCGGCAGCAGCCAGCCGCCTTCGCCGTCCATC               >  SRR4302542.585343/1‑94 (MQ=60)
                                                                               GTCAACTTTCGGGTGCGGGGTGAAGAATTTATCCAGATTATCGGTATGCAGCTCAATCAGCCCCGGCAGCAGCCAGCCGCCTTCGCCGTCCATC               <  SRR4302542.5564461/94‑1 (MQ=60)
                                                                               GTCAACTTTCGGGTGCGGGGTGAAGAATTTATCCAGATTATCGGTATGCAGCTCAATCAGCCCCGGCAGCAGCCAGCCGCCTTCGCCGTCCATC               >  SRR4302542.2843790/1‑94 (MQ=60)
                                                                               GTCAACTTTCGGGTGCGGGGTGAAGAATTTATCCAGATTATCGGTATGCAGCTCAATCAGCCCCGGCAGCAGCCAGCCGCCTTCGCCGTCCATC               >  SRR4302542.2906091/1‑94 (MQ=60)
                                                                                TCAACTTTCGGGTGCGGGGTGAAGAATTTATCCAGATTATCGGTATGCAGCTCAATCAGCCCCGGCAGCAGCCAGCCGCCTTCGCCGTCCATCG              <  SRR4302542.2543891/94‑1 (MQ=60)
                                                                                 CAACTTTCGGGTGCGGGGTGAAGAATTTATCCAGATTATCGGTATGCAGCTCAATCAGCCCCGGCAGCAGCCAGCCGCCTTCGCCGTCCATCGC             >  SRR4302542.2501721/1‑94 (MQ=60)
                                                                                  AACTTTCGGGTGCGGGGTGAAGAATTTATCCAGATTATCGGTATGCAGCTCAATCAGCCCCGGCAGCAGCCAGCCGCCTTCGCCGTCCATCGCC            >  SRR4302542.5431836/1‑94 (MQ=60)
                                                                                   ACTTTCGGGTGCGGGGTGAAGAATTTATCCAGATTATCGGTATGCAGCTCAATCAGCCCCGGCAGCAGCCAGCCGCCTTCGCCGTCCATCGCCT           <  SRR4302542.664873/94‑1 (MQ=60)
                                                                                    CTTTCGGGTGCGGGGTGAAGAATTTATCCAGATTATCGGTATGCAGCTCAATCAGCCCCGGCAGCAGCCAGCCGCCTTCGCCGTCCATCGCCTC          >  SRR4302542.2693976/1‑94 (MQ=60)
                                                                                     TTTCGGGTGCGGGGTGAAGAATTTATCCAGATTATCGGTATGCAGCTCAATCAGCCCCGGCAGCAGCCAGCCGCCTTCGCCGTCCATCGCCTCC         >  SRR4302542.5655438/1‑94 (MQ=60)
                                                                                     TTTCGGGTGCGGGGTGAAGAATTTATCCAGATTATCGGTATGCAGCTCAATCAGCCCCGGCAGCAGCCAGCCGCCTTCGCCGTCCATCGCCTCC         <  SRR4302542.5094662/94‑1 (MQ=60)
                                                                                      TTCGGGTGCGGGGTGAAGAATTTATCCAGATTATCGGTATGCAGCTCAATCAGCCCCGGCAGCAGCCAGCCGCCTTCGCCGTCCATCGCCTCCG        <  SRR4302542.4678556/94‑1 (MQ=60)
                                                                                      TTCGGGCGCGGGGTGAAGAATTTATCCAGATTATCGGTATGCAGCTCAATCAGCCCCGGCAGCAGCCAGCCGCCTTCGCCGTCCATCGCCTCCG        <  SRR4302542.825171/94‑1 (MQ=60)
                                                                                        CGGGTGCGGGGTGAAGAATTTATCCAGATTATCGGTATGCAGCTCAATCAGCCCCGGCAGCAGCCAGCCGCCTTCGCCGTCCATCGCCTCCGGC      >  SRR4302542.6389611/1‑94 (MQ=60)
                                                                                          GGTGCGGGGTGAAGAATTTATCCAGATTATCGGTATGCAGCTCAATCAGCCCCGGCAGCAGCCAGCCGCCTTCGCCGTCCATCGCCTCCGGCAG    >  SRR4302542.1525726/1‑94 (MQ=60)
                                                                                           GTGCGGGGTGAAGAATTTATCCAGATTATTGGTATGCAGCCCAACCAGCCCCGGCAGCGGCCAGCCGCCTTCGCCGTCCATCGCCTCCGGCAGG   <  SRR4302542.5255268/94‑1 (MQ=60)
                                                                                           GTGCGGGGTGAAGAATTTATCCAGATTATCGGTATGCAGCTCAATCAGCCCCGGCAGCAGCCAGCCGCCTTCGCCGTCCATCGCCTCCGGCAGG   <  SRR4302542.569553/94‑1 (MQ=60)
                                                                                           GCGCGGGGTGAAGAATTTATCCAGATTATCGGTATGCAGCTCAATCAGCCCCGGCAGCAGCCAGCCGCCTTCGCCGTCCATCGCCTCCGGCAGG   <  SRR4302542.5243403/94‑1 (MQ=60)
                                                                                            TGCGGGGTGAAGAATTTATCCAGATTATCGGTATGCAGCTCAATCAGCCCCGGCAGCAGCCAGCCGCCTTCGCCGTCCATCGCCTCCGGCAGGC  <  SRR4302542.6600245/94‑1 (MQ=60)
                                                                                            CGCGGGGTGAAGAATTTATCCAGATTATCGGTATGCAGCTCAATCAGCCCCGGCAGCAGCCAGCCGCCTTCGCCGTCCATCGCCTCCGGCAGGC  <  SRR4302542.6353386/94‑1 (MQ=60)
                                                                                            |                                                                                             
CCACGGCATCCAGTACGGTGGTGATGCCGCTCGCCACCATCAGCGCGTCGTGGCTGCTCATCGCCGAGTGGGCAGGCCAGTCAACTTTCGGGCGCGGGGTGAAGAATTTATCCAGATTATCGGTATGCAGCTCAATCAGCCCCGGCAGCAGCCAGCCGCCTTCGCCGTCCATCGCCTCCGGCAGGC  >  NC_000913/4314508‑4314693

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑