Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	1061	294.1	15065038	98.6%	14854127	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,334,019	A→G	L197L (CTT→CTC)	yjdE ←	putative amino acid/amine transport protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,334,019	0	A	G	81.9%	572.5 / 134.3	276	L197L (CTT→CTC)	yjdE	putative amino acid/amine transport protein
Reads supporting (aligned to +/- strand): ref base A (28/22); new base G (121/105); total (149/127)
Fisher's exact test for biased strand distribution p-value = 8.76e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.89e-01

Only 100 of 277 total aligned reads displayed.

GTGGCGATAGGGACATTGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTAAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATGCCGCCATATAGGTTTCACCACGGAACCAGAACCAGCCAAA  >  NC_000913/4333927‑4334108

gtggCGATAGGGACATTGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTa                                                                                           >  1:1247305/1‑93 (MQ=255)

  ggCGATAGGGACATTGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAg                                                                                         >  2:3715773/1‑93 (MQ=255)

  ggCGATAGGGACATTGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAg                                                                                         <  2:1450662/93‑1 (MQ=255)

  ggCGATAGGGACATTGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTAAg                                                                                         <  1:3467733/93‑1 (MQ=255)

   gCGATAGGGACATTGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTAAgg                                                                                        <  2:3590030/93‑1 (MQ=255)

    cGATAGGGACATTGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAggg                                                                                       <  2:4840437/93‑1 (MQ=255)

     gATAGGGACATTGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGt                                                                                      <  1:1677122/93‑1 (MQ=255)

     gATAGGGACATTGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGt                                                                                      >  2:2046608/1‑93 (MQ=255)

      aTAGGGACATTGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTa                                                                                     <  2:2345053/93‑1 (MQ=255)

       tAGGGACATTGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTAc                                                                                    >  2:1320007/1‑93 (MQ=255)

           gACATTGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTACTTTg                                                                                >  2:3618255/1‑93 (MQ=255)

            aCATTGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGa                                                                               <  2:5389349/93‑1 (MQ=255)

            aCATTGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGa                                                                               >  1:3975768/1‑93 (MQ=255)

             cATTGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGaa                                                                              <  1:5093264/93‑1 (MQ=255)

             cATTGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTAAGGGTACTTTGaa                                                                              <  1:5052146/93‑1 (MQ=255)

               ttGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAAtt                                                                            <  2:3838652/93‑1 (MQ=255)

                 gCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGc                                                                          <  2:3071292/93‑1 (MQ=255)

                 gCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGc                                                                          >  1:392383/1‑93 (MQ=255)

                 gCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGc                                                                          >  1:6197263/1‑93 (MQ=255)

                  cGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCa                                                                         >  1:4684562/1‑93 (MQ=255)

                   gTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCAc                                                                        >  1:6585671/1‑93 (MQ=255)

                   gTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCAc                                                                        <  1:321254/93‑1 (MQ=255)

                   gTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCAc                                                                        <  2:3295710/93‑1 (MQ=255)

                   gTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCAc                                                                        >  1:5563754/1‑93 (MQ=255)

                   gTTTCGGGGTTTTCACCACACCTGCGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGGACTTTGAATTGCAc                                                                        >  1:3734953/1‑93 (MQ=255)

                    tttCGGGTTTTTCACCACACCTGCGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTAAGGGTACTTTGAATTGCAcc                                                                       <  2:7162255/93‑1 (MQ=255)

                     ttCGGGTTTTTCACCACACCTGCGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCg                                                                      <  1:6519998/93‑1 (MQ=255)

                       cGGGTTTTTCACCACACCTGCGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGaa                                                                    <  1:1907198/93‑1 (MQ=255)

                       cGGGTTTTTCACCACACCTGCGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGaa                                                                    <  1:6006664/93‑1 (MQ=255)

                         ggTTTTTCACCACACCTGCGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAgg                                                                  <  2:4546759/93‑1 (MQ=255)

                          gTTTTTCACCACACCTGCGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGt                                                                 <  2:5975316/93‑1 (MQ=255)

                          gTTTTTCACCACACCTGCGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTAAGGGTACTTTGAATTGCACCGAAGGt                                                                 <  1:3690221/93‑1 (MQ=255)

                            ttttCACCACACCTGCGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTAAGGGTACTTTGAATTGCACCGAAGGTGc                                                               >  1:6663656/1‑93 (MQ=255)

                             tttCACCACACCTGCGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGcc                                                              >  1:4474403/1‑93 (MQ=255)

                               tCACCACACCTGCGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCa                                                            <  1:605796/93‑1 (MQ=255)

                               tCACCACACCTGCGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCa                                                            >  1:4094357/1‑93 (MQ=255)

                               tCACCACACCTGCGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCa                                                            >  2:1926074/1‑93 (MQ=255)

                               tCACCACACCTGCGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCa                                                            <  2:5093008/93‑1 (MQ=255)

                               tCACCACACCTGCGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTAAGGGTACTTTGAATTGCACCGAAGGTGCCCa                                                            <  1:5293815/93‑1 (MQ=255)

                                caccacACCTGCGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAg                                                           >  1:4224543/1‑93 (MQ=255)

                                 accacACCTGCGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAgg                                                          >  2:700340/1‑93 (MQ=255)

                                 accacACCTGCGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAgg                                                          <  1:801422/93‑1 (MQ=255)

                                     cacCTGCGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGc                                                      >  1:3675511/1‑93 (MQ=255)

                                     cacCTGCGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGc                                                      <  1:3563844/93‑1 (MQ=255)

                                        cTGCGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGa                                                   >  1:6987977/1‑93 (MQ=255)

                                        cTGCGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTAAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGa                                                   >  1:1467943/1‑93 (MQ=255)

                                          gCGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACg                                                 <  2:4105054/93‑1 (MQ=255)

                                           cGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGt                                                <  1:6351115/93‑1 (MQ=255)

                                           cGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGt                                                <  2:6683777/93‑1 (MQ=255)

                                              cAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTcc                                             <  2:1714531/93‑1 (MQ=255)

                                               aaCGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCa                                            >  2:349919/1‑93 (MQ=255)

                                               aaCGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCa                                            <  2:5857988/93‑1 (MQ=255)

                                                aCGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCAt                                           >  2:6261165/1‑93 (MQ=255)

                                                aCGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCAt                                           >  2:6749457/1‑93 (MQ=255)

                                                aCGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCAt                                           <  1:286146/93‑1 (MQ=255)

                                                aCGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCAt                                           >  2:1288594/1‑93 (MQ=255)

                                                  ggAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATgc                                         <  1:7342659/93‑1 (MQ=255)

                                                   gAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATgcc                                        <  2:4274559/93‑1 (MQ=255)

                                                   gAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATgcc                                        >  2:2918123/1‑93 (MQ=255)

                                                      gCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATgccgcc                                     <  2:2635193/93‑1 (MQ=255)

                                                        aCTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATGCCGCCat                                   >  2:4442577/1‑93 (MQ=255)

                                                         cTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATGCCGCCata                                  >  2:1579103/1‑93 (MQ=255)

                                                         cTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATGCCGCCata                                  >  2:5262036/1‑93 (MQ=255)

                                                          tttCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATGCCGCCatat                                 <  1:7061117/93‑1 (MQ=255)

                                                          tttCCACACCGATGAACGACCACAGCGTAACGTTAAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATGCCGCCatat                                 >  1:3003390/1‑93 (MQ=255)

                                                           ttCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATGCCGCCAtata                                <  2:2866588/93‑1 (MQ=255)

                                                           ttCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATGCCGCCAtata                                <  2:68311/93‑1 (MQ=255)

                                                           ttCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATGCCGCCAtata                                <  2:2729850/93‑1 (MQ=255)

                                                           ttCCACACCGATGAACGACCACAGCGTAACGTTAAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATGCCGCCAtata                                <  2:2835763/93‑1 (MQ=255)

                                                           ttCCACACCGATGAACGACCACAGCGTAACGTTAAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATGCCGCCAtata                                >  2:1040707/1‑93 (MQ=255)

                                                            tCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATGCCGCCATATAg                               <  1:1011685/93‑1 (MQ=255)

                                                              cacaCCGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATGCCGCCATATAGGt                             <  2:463436/93‑1 (MQ=255)

                                                               acacCGATGAACGACCACAGCGTAACGTTAAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATGCCGCCATATAGGtt                            >  1:721954/1‑93 (MQ=255)

                                                                cacCGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATGCCGCCATATAGGttt                           >  1:244744/1‑93 (MQ=255)

                                                                cacCGATGAACGACCACAGCGTAACGTTAAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATGCCGCCATATAGGttt                           >  2:7399835/1‑93 (MQ=255)

                                                                  ccGATGAACGACCACAGCGTAACGTTGATGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATGCCGCCATATAGGTTTca                         <  2:2153635/93‑1 (MQ=255)

                                                                  ccGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATGCCGCCATATAGGTTTca                         <  2:5515164/93‑1 (MQ=255)

                                                                   cGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTTACGTTCCATGCCGCCATATAGGTTTtac                        >  1:2380050/1‑93 (MQ=255)

                                                                   cGATGAACGACCACAGCGTAACGTTAAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATGCCGCCATATAGGTTTcac                        >  2:6564021/1‑93 (MQ=255)

                                                                    gATGAACGACCACAGCGTAACGTTGGGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATGCCGCCATATAGGTTTcacc                       >  2:643677/1‑93 (MQ=255)

                                                                    gATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATGCCGCCATATAGGTTTcacc                       >  2:4101582/1‑93 (MQ=255)

                                                                    gATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATGCCGCCATATAGGTTTcacc                       >  1:6809601/1‑93 (MQ=255)

                                                                     aTGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATGCCGCCATATAGGTTTcacca                      <  2:5718383/93‑1 (MQ=255)

                                                                      tGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATGCCGCCATATAGGTTTcaccac                     <  2:3196497/93‑1 (MQ=255)

                                                                      tGAACGACCACAGCGTAACGTTAAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATGCCGCCATATAGGTTTcaccac                     >  1:921742/1‑93 (MQ=255)

                                                                         aCGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATGCCGCCATATAGGTTTCACCACGga                  >  1:1024991/1‑93 (MQ=255)

                                                                         aCGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATGCCGCCATATAGGTTTCACCACGga                  >  2:2581250/1‑93 (MQ=255)

                                                                         aCGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATGCCGCCATATAGGTTTCACCACGga                  <  2:1878861/93‑1 (MQ=255)

                                                                          cGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATGCCGCCATATAGGTTTCACCACGgaa                 <  2:5641434/93‑1 (MQ=255)

                                                                          cGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATGCCGCCATATAGGTTTCACCACGgaa                 <  1:6840148/93‑1 (MQ=255)

                                                                           gACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATGCCGCCATATAGGTTTCACCACGgaac                <  2:4024081/93‑1 (MQ=255)

                                                                            aCCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATGCCGCCATATAGGTTTCACCACGgaacc               <  1:6284711/93‑1 (MQ=255)

                                                                             ccACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATGCCGCCATATAGGTTTCACCACGgaacca              <  2:5801764/93‑1 (MQ=255)

                                                                             ccACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATGCCGCCATATAGGTTTCACCACGgaacca              >  1:4294306/1‑93 (MQ=255)

                                                                             ccACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATGCCGCCATATAGGTTTCACCACGgaacca              >  1:2163415/1‑93 (MQ=255)

                                                                              cacaGCGTAACGTTAAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATGCCGCCATATAGGTTTCACCACGgaaccag             <  2:852037/93‑1 (MQ=255)

                                                                                 aGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATGCCGCCATATAGGTTTCACCACGgaaccagaac          >  1:4941195/1‑93 (MQ=255)

                                                                                   cGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATGCCGCCATATAGGTTTCACCACGgaaccagaacca        <  1:6922171/93‑1 (MQ=255)

                                                                                    gTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATGCCGCCATATAGGTTTCACCACGGaaccagaaccag       >  2:3146722/1‑93 (MQ=255)

                                                                                         gTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATGCCGCCATATAGGTTTCACCACGGAACCAGAACCAGCCaaa  >  2:514357/1‑93 (MQ=255)

GTGGCGATAGGGACATTGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTAAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATGCCGCCATATAGGTTTCACCACGGAACCAGAACCAGCCAAA  >  NC_000913/4333927‑4334108

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

GGTGGCGATAGGGACATTGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTAAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATGCCGCCATATAGGTTTCACCACGGAACCAGAACCAGCCAAATACG  >  NC_000913/4333926‑4334112

GGTGGCGATAGGGACATTGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTG                                                                                               <  SRR4302542.5805737/94‑1 (MQ=60)

GGTGGCGATAGGGACATTGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTG                                                                                               >  SRR4302542.5328054/1‑94 (MQ=60)

GGTGGCGATAGGGACATTGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTA                                                                                               >  SRR4302542.1258026/1‑94 (MQ=60)

  TGGCGATAGGGACATTGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAG                                                                                             <  SRR4302542.2772643/94‑1 (MQ=60)

  TGGCGATAGGGACATTGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAG                                                                                             >  SRR4302542.2291387/1‑94 (MQ=60)

  TGGCGATAGGGACATTGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAG                                                                                             <  SRR4302542.4511078/94‑1 (MQ=60)

   GGCGATAGGGACATTGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGG                                                                                            >  SRR4302542.2989789/1‑94 (MQ=60)

   GGCGATAGGGACATTGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGG                                                                                            <  SRR4302542.400357/94‑1 (MQ=60)

   GGCGATAGGGACATTGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTAAGG                                                                                            <  SRR4302542.3819392/94‑1 (MQ=60)

   GGCGATAGGGACATTGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTAAGG                                                                                            <  SRR4302542.3500206/94‑1 (MQ=60)

    GCGATAGGGACATTGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGG                                                                                           >  SRR4302542.3758640/1‑94 (MQ=60)

    GCGATAGGGACATTGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTAAGGG                                                                                           >  SRR4302542.7052662/1‑94 (MQ=60)

      GATAGGGACATTGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTA                                                                                         <  SRR4302542.1691618/94‑1 (MQ=60)

      GATAGGGACATTGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTAAGGGTA                                                                                         <  SRR4302542.862329/94‑1 (MQ=60)

      GATAGGGACATTGCGTCTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTAAGGGTA                                                                                         >  SRR4302542.5391942/1‑94 (MQ=60)

       ATAGGGACATTGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTAC                                                                                        >  SRR4302542.6410593/1‑94 (MQ=60)

         AGGGACATTGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTACTT                                                                                      <  SRR4302542.5312958/94‑1 (MQ=60)

         AGGGACATTGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTACTT                                                                                      >  SRR4302542.7295829/1‑94 (MQ=60)

         AGGGACATTGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTAAGGGTACTT                                                                                      <  SRR4302542.977344/94‑1 (MQ=60)

          GGGACATTGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTACTTT                                                                                     >  SRR4302542.7109795/1‑94 (MQ=60)

           GGACATTGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTACTTTG                                                                                    <  SRR4302542.6683163/94‑1 (MQ=60)

           GGACATTGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTACTTTG                                                                                    >  SRR4302542.844638/1‑94 (MQ=60)

            GACATTGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGA                                                                                   >  SRR4302542.4014995/1‑94 (MQ=60)

            GACATTGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGA                                                                                   >  SRR4302542.5080329/1‑94 (MQ=60)

              CATTGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAAT                                                                                 <  SRR4302542.5142764/94‑1 (MQ=60)

              CATTGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAAT                                                                                 >  SRR4302542.3513587/1‑94 (MQ=60)

              CATTGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTAAGGGTACTTTGAAT                                                                                 <  SRR4302542.5101204/94‑1 (MQ=60)

               ATTGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTAAGGGTACTTTGAATT                                                                                <  SRR4302542.6896826/94‑1 (MQ=60)

                 TGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGC                                                                              >  SRR4302542.6257743/1‑94 (MQ=60)

                 TGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGC                                                                              >  SRR4302542.395778/1‑94 (MQ=60)

                 TGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGC                                                                              >  SRR4302542.6257744/1‑94 (MQ=60)

                 TGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTAAGGGTACTTTGAATTGC                                                                              <  SRR4302542.6676148/94‑1 (MQ=60)

                  GCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCA                                                                             >  SRR4302542.4730322/1‑94 (MQ=60)

                  GCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCA                                                                             <  SRR4302542.3445527/94‑1 (MQ=60)

                   CGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCAC                                                                            >  SRR4302542.5617816/1‑94 (MQ=60)

                   CGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCAC                                                                            >  SRR4302542.6650555/1‑94 (MQ=60)

                   CGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCAC                                                                            <  SRR4302542.1074978/94‑1 (MQ=60)

                   CGTTTCGGGGTTTTCACCACACCTGCGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGGACTTTGAATTGCAC                                                                            >  SRR4302542.3771931/1‑94 (MQ=60)

                    GTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACC                                                                           <  SRR4302542.324043/94‑1 (MQ=60)

                    GTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACC                                                                           <  SRR4302542.4110983/94‑1 (MQ=60)

                    GTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACC                                                                           >  SRR4302542.3602519/1‑94 (MQ=60)

                    GTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACC                                                                           >  SRR4302542.6316370/1‑94 (MQ=60)

                    GTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACC                                                                           >  SRR4302542.3766368/1‑94 (MQ=60)

                      TTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGA                                                                         <  SRR4302542.6584247/94‑1 (MQ=60)

                        CGGGTTTTTCACCACACCTGCGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAG                                                                       >  SRR4302542.2488372/1‑94 (MQ=60)

                        CGGGTTTTTCACCACACCTGCGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAG                                                                       >  SRR4302542.1198432/1‑94 (MQ=60)

                        CGGGTTTTTCACCACACCTGCGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAG                                                                       <  SRR4302542.1923678/94‑1 (MQ=60)

                        CGGGTTTTTCACCACACCTGCGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAG                                                                       <  SRR4302542.6064834/94‑1 (MQ=60)

                        CGGGTTTTTCACCACACCTGCGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAG                                                                       >  SRR4302542.5916587/1‑94 (MQ=60)

                        CGGGTTTTTCACCACACCTGCGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTAAGGGTACTTTGAATTGCACCGAAG                                                                       <  SRR4302542.4531719/94‑1 (MQ=60)

                         GGGTTTTTCACCACACCTGCGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGG                                                                      >  SRR4302542.636762/1‑94 (MQ=60)

                          GGTTTTTCACCACACCTGCGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGT                                                                     >  SRR4302542.6107498/1‑94 (MQ=60)

                          GGTTTTTCACCACACCTGCGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGT                                                                     <  SRR4302542.656830/94‑1 (MQ=60)

                          GGTTTTTCACCACACCTGCGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGT                                                                     <  SRR4302542.1024925/94‑1 (MQ=60)

                           GTTTTTCACCACACCTGCGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTG                                                                    <  SRR4302542.5549323/94‑1 (MQ=60)

                           GTTTTTCACCACACCTGCGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTAAGGGTACTTTGAATTGCACCGAAGGTG                                                                    <  SRR4302542.3726738/94‑1 (MQ=60)

                            TTTTTCACCACACCTGCGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGC                                                                   >  SRR4302542.6620269/1‑94 (MQ=60)

                            TTTTTCACCACACCTGCGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTAAGGGTACTTTGAATTGCACCGAAGGTGC                                                                   >  SRR4302542.6729309/1‑94 (MQ=60)

                             TTTTCACCACACCTGCGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCC                                                                  >  SRR4302542.4518286/1‑94 (MQ=60)

                              ttcccccccaccgGCGGCAACGGGGGCACTTTCCCCACCGAGGAACGACCACAGCGTAACGTGGAGGGTCTTTGGAATTGCCCCGAGGGTGCCC                                                                 <  SRR4302542.1251137/81‑1 (MQ=26)

                               TTCACCACACCTGCGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCA                                                                >  SRR4302542.4134742/1‑94 (MQ=60)

                                TCACCACACCTGCGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAG                                                               >  SRR4302542.4266165/1‑94 (MQ=60)

                                TCACCACACCTGCGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAG                                                               <  SRR4302542.611011/94‑1 (MQ=60)

                                TCACCACACCTGCGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTAAGGGTACTTTGAATTGCACCGAAGGTGCCCAG                                                               <  SRR4302542.5345260/94‑1 (MQ=60)

                                 CACCACACCTGCGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTAAGGGTACTTTGAATTGCACCGAAGGTGCCCAGG                                                              >  SRR4302542.3158154/1‑94 (MQ=60)

                                  ACCACACCTGCGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGC                                                             <  SRR4302542.808314/94‑1 (MQ=60)

                                  ACCACACCTGCGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTAAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGC                                                             <  SRR4302542.653983/94‑1 (MQ=60)

                                   CCACACCTGCGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCC                                                            <  SRR4302542.205723/94‑1 (MQ=60)

                                   CCACACCTGCGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCC                                                            >  SRR4302542.4970010/1‑94 (MQ=60)

                                   CCACACCTGCGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCC                                                            <  SRR4302542.5079794/94‑1 (MQ=60)

                                    CACACCTGCGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCG                                                           >  SRR4302542.427210/1‑94 (MQ=60)

                                     ACACCTGCGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGC                                                          >  SRR4302542.3711152/1‑94 (MQ=60)

                                      CACCTGCGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCT                                                         <  SRR4302542.3597119/94‑1 (MQ=60)

                                       ACCTGCGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTAAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTG                                                        >  SRR4302542.242409/1‑94 (MQ=60)

                                        CCTGCGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGA                                                       >  SRR4302542.7056619/1‑94 (MQ=60)

                                        CCTGCGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTAAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGA                                                       >  SRR4302542.1480617/1‑94 (MQ=60)

                                         CTGCGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGAC                                                      <  SRR4302542.7076921/94‑1 (MQ=60)

                                         CTGCGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGAC                                                      >  SRR4302542.5930263/1‑94 (MQ=60)

                                         CTGCGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGAC                                                      <  SRR4302542.1403769/94‑1 (MQ=60)

                                         CTGCGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGAC                                                      >  SRR4302542.6882228/1‑94 (MQ=60)

                                            CGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTT                                                   <  SRR4302542.6413681/94‑1 (MQ=60)

                                             GGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTAAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTC                                                  >  SRR4302542.6768004/1‑94 (MQ=60)

                                               CAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCA                                                >  SRR4302542.4653766/1‑94 (MQ=60)

                                               CAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTAAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCA                                                >  SRR4302542.6073893/1‑94 (MQ=60)

                                                AACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCAT                                               >  SRR4302542.4316290/1‑94 (MQ=60)

                                                AACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCAT                                               <  SRR4302542.6913568/94‑1 (MQ=60)

                                                 ACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATG                                              <  SRR4302542.5125468/94‑1 (MQ=60)

                                                 ACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATG                                              <  SRR4302542.288640/94‑1 (MQ=60)

                                                   GGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATGCC                                            <  SRR4302542.7414579/94‑1 (MQ=60)

                                                        CACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATGCCGCCAT                                       <  SRR4302542.3143057/94‑1 (MQ=60)

                                                         ACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATGCCGCCATA                                      >  SRR4302542.4787603/1‑94 (MQ=60)

                                                          CTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATGCCGCCATAT                                     >  SRR4302542.420172/1‑94 (MQ=60)

                                                          CTTTCCACACCGATGAACGACCACAGCGTAACGTTAAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATGCCGCCATAT                                     >  SRR4302542.3032113/1‑94 (MQ=60)

                                                           TTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATGCCGCCATATA                                    <  SRR4302542.7130425/94‑1 (MQ=60)

                                                            TTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATGCCGCCATATAG                                   >  SRR4302542.1266233/1‑94 (MQ=60)

                                                            TTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATGCCGCCATATAG                                   <  SRR4302542.4016068/94‑1 (MQ=60)

                                                            TTCCACACCGATGAACGACCACAGCGTAACGTTAAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATGCCGCCATATAG                                   <  SRR4302542.1985226/94‑1 (MQ=60)

                                                             TCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATGCCGCCATATAGG                                  <  SRR4302542.1020397/94‑1 (MQ=60)

                                                             TCCACACCGATGAACGACCACAGCGTAACGCTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATGCCGCCATATAGG                                  >  SRR4302542.3719353/1‑94 (MQ=60)

                                                               CACACCGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATGCCGCCATATAGGTT                                >  SRR4302542.2241520/1‑94 (MQ=60)

                                                               CACACCGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATGCCGCCATATAGGTT                                >  SRR4302542.7283243/1‑94 (MQ=60)

                                                               CACACCGATGAACGACCACAGCGTAACGTTAAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATGCCGCCATATAGGTT                                >  SRR4302542.728175/1‑94 (MQ=60)

                                                                ACACCGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATGCCGCCATATAGGTTT                               >  SRR4302542.246845/1‑94 (MQ=60)

                                                                ACACCGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATGCCGCCATATAGGTTT                               >  SRR4302542.929802/1‑94 (MQ=60)

                                                                  ACCGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATGCCGCCATATAGGTTTCA                             >  SRR4302542.3415910/1‑94 (MQ=60)

                                                                   CCGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTTACGTTCCATGCCGCCATATAGGTTTTAC                            >  SRR4302542.2401677/1‑94 (MQ=60)

                                                                   CCGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATGCCGCCATATAGGTTTCAC                            >  SRR4302542.2914516/1‑94 (MQ=60)

                                                                    CGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATGCCGCCATATAGGTTTCACC                           <  SRR4302542.1564649/94‑1 (MQ=60)

                                                                    CGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATGCCGCCATATAGGTTTCACC                           >  SRR4302542.6876549/1‑94 (MQ=60)

                                                                    CGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATGCCGCCATATAGGTTTCACC                           >  SRR4302542.6876548/1‑94 (MQ=60)

                                                                      ATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATGCCGCCATATAGGTTTCACCAC                         >  SRR4302542.6261780/1‑94 (MQ=60)

                                                                      ATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATGCCGCCATATAGGTTTCACCAC                         <  SRR4302542.3592310/94‑1 (MQ=60)

                                                                      ATGAACGACCACAGCGTAACGTTAAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATGCCGCCATATAGGTTTCACCAC                         >  SRR4302542.929677/1‑94 (MQ=60)

                                                                      ATGAACGACCACAGCGTAACGTTAAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATGCCGCCATATAGGTTTCACCAC                         >  SRR4302542.745293/1‑94 (MQ=60)

                                                                         AACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATGCCGCCATATAGGTTTCACCACGGA                      <  SRR4302542.1779445/94‑1 (MQ=60)

                                                                         AACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATGCCGCCATATAGGTTTCACCACGGA                      >  SRR4302542.1033822/1‑94 (MQ=60)

                                                                         AACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATGCCGCCATATAGGTTTCACCACGGA                      <  SRR4302542.7273382/94‑1 (MQ=60)

                                                                           CGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATGCCGCCATATAGGTTTCACCACGGAAC                    <  SRR4302542.6907352/94‑1 (MQ=60)

                                                                             ACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATGCCGCCATATAGGTTTCACCACGGAACCA                  >  SRR4302542.2182046/1‑94 (MQ=60)

                                                                             ACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATGCCGCCATATAGGTTTCACCACGGAACCA                  >  SRR4302542.4336543/1‑94 (MQ=60)

                                                                             ACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATGCCGCCATATAGGTTTCACCACGGAACCA                  <  SRR4302542.6346641/94‑1 (MQ=60)

                                                                             ACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATGCCGCCATATAGGTTTCACCACGGAACCA                  >  SRR4302542.4461236/1‑94 (MQ=60)

                                                                             ACCACAGCGTAACGTTAAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATGCCGCCATATAGGTTTCACCACGGAACCA                  >  SRR4302542.245865/1‑94 (MQ=60)

                                                                              CCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATGCCGCCATATAGGTTTCACCACGGAACCAG                 >  SRR4302542.6975850/1‑94 (MQ=60)

                                                                               CACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATGCCGCCATATAGGTTTCACCACGGAACCAGA                >  SRR4302542.5122114/1‑94 (MQ=60)

                                                                                 CAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATGCCGCCATATAGGTTTCACCACGGAACCAGAAC              >  SRR4302542.4989212/1‑94 (MQ=60)

                                                                                  AGCGTAACGTTAAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATGCCGCCATATAGGTTTCACCACGGAACCAGAACC             >  SRR4302542.3717667/1‑94 (MQ=60)

                                                                                   GCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATGCCGCCATATAGGTTTCACCACGGAACCAGAACCA            >  SRR4302542.3871443/1‑94 (MQ=60)

                                                                                    CGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATGCCGCCATATAGGTTTCACCACGGAACCAGAACCAG           <  SRR4302542.6990132/94‑1 (MQ=60)

                                                                                      TAACGTTAAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATGCCGCCATATAGGTTTCACCACGGAACCAGAACCAGCC         >  SRR4302542.7394821/1‑94 (MQ=60)

                                                                                       AACGTTAAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATGCCGCCATATAGGTTTCACCACGGAACCAGAACCAGCCA        >  SRR4302542.1663968/1‑94 (MQ=60)

                                                                                          GTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATGCCGCCATATAGGTTTCACCACGGAACCAGAACCAGCCAAAT     >  SRR4302542.5760954/1‑94 (MQ=60)

                                                                                          GTTAAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATGCCGCCATATAGGTTTCACCACGGAACCAGAACCAGCCAAAT     <  SRR4302542.4205082/94‑1 (MQ=60)

                                                                                             GAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATGCCGCCATATAGGTTTCACCACGGAACCAGAACCAGCCAAATACG  <  SRR4302542.4438469/94‑1 (MQ=60)

                                                                                             GAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATGCCGCCATATAGGTTTCACCACGGAACCAGAACCAGCCAAATACG  <  SRR4302542.3018134/94‑1 (MQ=60)

                                                                                             GAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATGCCGCCATATAGGTTTCACCACGGAACCAGAACCAGCCAAATACG  <  SRR4302542.2903913/94‑1 (MQ=60)

GGTGGCGATAGGGACATTGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTAAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATGCCGCCATATAGGTTTCACCACGGAACCAGAACCAGCCAAATACG  >  NC_000913/4333926‑4334112

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`