Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1061 294.1 15065038 98.6% 14854127 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,444,741 A→G E1018G (GAG→GGG)  ytfN → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,444,7410AG86.9% 705.4 / 96.0 289E1018G (GAG→GGG) ytfNhypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (18/20);  new base G (119/132);  total (137/152)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.83e-01

Only 100 of 294 total aligned reads displayed.

TTGGGCGCGCATCGTGGTGCACGATCTGCCGGAAAGCGCAGTAGGCGTCTCCAGCGATGTGGTGATGCTTAACGATAACCTGCAACCGGAAGAGCCGAAAACGGCGTCGATTCCGATTAACAGTAACCTGATTGTCCACGTTGGCAACAATGTGCGCATTGACGCCTTTGGCCTGAAAGCGCGG  >  NC_000913/4444649‑4444832
                                                                                            |                                                                                           
  gggCGCGCATCGTGGTGCACGATCTGCCGGAAAGCGCAGTAGGCGTCTCCAGCGATGTGGTGATGCTTAACGATAACCTGCAACCGGAAGGGc                                                                                           <  1:3217237/93‑1 (MQ=255)
   ggCGCGCATCGTGGTGCACGATCTGCCGGAAAGCGCAGTAGGCGTCTCCAGCGATGTGGTGATGCTTAACGATAACCTGCAACCGGAAGGGcc                                                                                          <  2:6591749/93‑1 (MQ=255)
   ggCGCGCATCGTGGTGCACGATCTGCCGGAAAGCGCAGTAGGCGTCTCCAGCGATGTGGTGATGCTTAACGATAACCTGCAACCGGAAGGGcc                                                                                          <  1:254035/93‑1 (MQ=255)
      gcgcATCGTGGTGCACGATCTGCCGGAAAGCGCAGTAGGCGTCTCCAGCGATGTGGTGATGCTTAACGATAACCTGCAACCGGAAGGGCCGaa                                                                                       >  1:1453196/1‑93 (MQ=255)
       cgcATCGTGGTGCACGATCTGCCGGAAAGCGCAGTAGGCGTCTCCAGCGATGTGGTGATGCTTAACGATAACCTGCAACCGGAAGGGCCGaaa                                                                                      <  2:3216006/93‑1 (MQ=255)
        gcATCGTGGTGCACGATCTGCCGGAAAGCGCAGTAGGCGTCTCCAGCGATGTGGTGATGCTTAACGATAACCTGCAACCGGAAGGGCCGaaaa                                                                                     >  2:2369130/1‑93 (MQ=255)
        gcATCGTGGTGCACGATCTGCCGGAAAGCGCAGTAGGCGTCTCCAGCGATGTGGTGATGCTTAACGATAACCTGCAACCGGAAGGGCCGaaaa                                                                                     <  1:5586050/93‑1 (MQ=255)
        gcATCGTGGTGCACGATCTGCCGGAAAGCGCAGTAGGCGTCTCCAGCGATGTGGTGATGCTTAACGATAACCTGCAACCGGAAGGGCCGaaaa                                                                                     <  2:6560397/93‑1 (MQ=255)
        gcATCGTGGTGCACGATCTGCCGGAAAGCGCAGTAGGCGTCTCCAGCGATGTGGTGATGCTTAACGATAACCTGCAACCGGAAGAGCCGaaaa                                                                                     <  1:950492/93‑1 (MQ=255)
         cATCGTGGTGCACGATCTGCCGGAAAGCGCAGTAGGCGTCTCCAGCGATGTGGTGATGCTTAACGATAACCTGCAACCGGAAGGGCCGAAAAc                                                                                    <  1:5137823/93‑1 (MQ=255)
           tCGTGGTGCACGATCTGCCGGAAAGCGCAGTAGGCGTCTCCAGCGATGTGGTGATGCTTAACGATAACCTGCAACCGGAAGGGCCGAAAACgg                                                                                  <  2:4009716/93‑1 (MQ=255)
           tCGTGGTGCACGATCTGCCGGAAAGCGCAGTAGGCGTCTCCAGCGATGTGGTGATGCTTAACGATAACCTGCAACCGGAAGGGCCGAAAACgg                                                                                  <  1:2670818/93‑1 (MQ=255)
           tCGTGGTGCACGATCTGCCGGAAAGCGCAGTAGGCGTCTCCAGCGATGTGGTGATGCTTAACGATAACCTGCAACCGGAAGGGCCGAAAACgg                                                                                  >  1:6430420/1‑93 (MQ=255)
              tggtgCACGATCTGCCGGAAAGCGCAGTAGGCGTCTCCAGCGATGTGGTGATGCTTAACGATAACCTGCAACCGGAAGGGCCGAAAACGGCGt                                                                               <  2:3942939/93‑1 (MQ=255)
              tggtgCACGATCTGCCGGAAAGCGCAGTAGGCGTCTCCAGCGATGTGGTGATGCTTAACGATAACCTGCAACCGGAAGGGCCGAAAACGGCGt                                                                               <  1:508746/93‑1 (MQ=255)
               ggtgCACGATCTGCCGGAAAGCGCAGTAGGCGTCTCCAGCGATGTGGTGATGCTTAACGATAACCTGCAACCGGAAGGGCCGAAAACGGCGTc                                                                              <  2:268019/93‑1 (MQ=255)
                gtgCACGATCTGCCGGAAAGCGCAGTAGGCGTCTCCAGCGATGTGGTGATGCTTAACGATAACCTGCAACCGGAAGGGCCGAAAACGGCGTCg                                                                             <  2:7251456/93‑1 (MQ=255)
                gtgCACGATCTGCCGGAAAGCGCAGTAGGCGTCTCCAGCGATGTGGTGATGCTTAACGATAACCTGCAACCGGAAGAGCCGAAAACGGCGTCg                                                                             >  2:5295660/1‑93 (MQ=255)
                 tgCACGATCTGCCGGAAAGCGCAGTAGGCGTCTCCAGCGATGTGGTGATGCTTAACGATAACCTGCAACCGGAAGGGCCGAAAACGGCGTCGa                                                                            <  1:2731869/93‑1 (MQ=255)
                    aCGATCTGCCGGAAAGCGCAGTAGGCGTCTCCAGCGATGTGGTGATGCTTAACGATAACCTGCAACCGGAAGGGCCGAAAACGGCGTCGATTc                                                                         >  1:901415/1‑93 (MQ=255)
                    aCGATCTGCCGGAAAGCGCAGTAGGCGTCTCCAGCGATGTGGTGATGCTTAACGATAACCTGCAACCGGAAGAGCCGAAAACGGCGTCGATTc                                                                         <  1:755160/93‑1 (MQ=255)
                     cGATCTGCCGGAAAGCGCAGTAGGCGTCTCCAGCGATGTGGTGATGCTTAACGATAACCTGCAACCGGAAGGGCCGAAAACGGCGTCGATTcc                                                                        <  2:3833878/93‑1 (MQ=255)
                     cGATCTGCCGGAAAGCGCAGTAGGCGTCTCCAGCGATGTGGTGATGCTTAACGATAACCTGCAACCGGAAGGGCCGAAAACGGCGTCGATTcc                                                                        >  1:2893914/1‑93 (MQ=255)
                      gATCTGCCGGAAAGCGCAGTAGGCGTCTCCAGCGATGTGGTGATGCTTAACGATAACCTGCAACCGGAAGGGCCGAAAACGGCGTCGATTCCg                                                                       >  2:3575464/1‑93 (MQ=255)
                      gATCTGCCGGAAAGCGCAGTAGGCGTCTCCAGCGATGTGGTGATGCTTAACGATAACCTGCAACCGGAAGGGCCGAAAACGGCGTCGATTCCg                                                                       >  1:4661026/1‑93 (MQ=255)
                       aTCTGCCGGAAAGCGCAGTAGGCGTCTCCAGCGATGTGGTGATGCTTAACGATAACCTGCAACCGGAAGGGCCGAAAACGGCGTCGATTCCGa                                                                      <  1:3011704/93‑1 (MQ=255)
                       aTCTGCCGGAAAGCGCAGTAGGCGTCTCCAGCGATGTGGTGATGCTTAACGATAACCTGCAACCGGAAGGGCCGAAAACGGCGTCGATTCCGa                                                                      >  1:3782535/1‑93 (MQ=255)
                       aTCTGCCGGAAAGCGCAGTAGGCGTCTCCAGCGATGTGGTGATGCTTAACGATAACCTGCAACCGGAAGAGCCGAAAACGGCGTCGATTCCGa                                                                      >  1:585498/1‑93 (MQ=255)
                        tCTGCCGGAAAGCGCAGTAGGCGTCTCCAGCGATGTGGTGATGCTTAACGATAACCTGCAACCGGAAGGGCCGAAAACGGCGTCGATTCCGAt                                                                     >  1:207918/1‑93 (MQ=255)
                        tCTGCCGGAAAGCGCAGTAGGCGTCTCCAGCGATGTGGTGATGCTTAACGATAACCTGCAACCGGAAGGGCCGAAAACGGCGTCGATTCCGAt                                                                     <  1:3487390/93‑1 (MQ=255)
                         cTGCCGGAAAGCGCAGTAGGCGTCTCCAGCGATGTGGTGATGCTTAACGATAACCTGCAACCGGAAGGGCCGAAAACGGCGTCGATTCCGAtt                                                                    >  1:1743205/1‑93 (MQ=255)
                          tGCCGGAAAGCGCAGTAGGCGTCTCCAGCGATGTGGTGATGCTTAACGATAACCTGCAACCGGAAGGGCCGAAAACGGCGTCGATTCCGATTa                                                                   <  2:5496226/93‑1 (MQ=255)
                           gCCGGAAAGCGCAGTAGGCGTCTCCAGCGATGTGGTGATGCTTAACGATAACCTGCAACCGGAAGGGCCGAAAACGGCGTCGATTCCGATTaa                                                                  >  1:4925916/1‑93 (MQ=255)
                              ggAAAGCGCAGTAGGCGTCTCCAGCGATGTGGTGATGCTTAACGATAACCTGCAACCGGAAGGGCCGAAAACGGCGTCGATTCCGATTAACAg                                                               <  1:1084933/93‑1 (MQ=255)
                              ggAAAGCGCAGTAGGCGTCTCCAGCGATGTGGTGATGCTTAACGATAACCTGCAACCGGAAGGGCCGAAAACGGCGTCGATTCCGATTAACAg                                                               <  2:16336/93‑1 (MQ=255)
                              ggAAAGCGCAGTAGGCGTCTCCAGCGATGTGGTGATGCTTAACGATAACCTGCAACCGGAAGAGCCGAAAACGGCGTCGATTCCGATTAACAg                                                               <  2:6320348/93‑1 (MQ=255)
                              ggAAAGCGCAGTAGGCGTCTCCAGCGATGTGGTGATGCTTAACGATAACCTGCAACCGGAAGAGCCGAAAACGGCGTCGATTCCGATTAACAg                                                               >  2:4073945/1‑93 (MQ=255)
                                  aGCGCAGTAGGCGTCTCCAGCGATGTGGTGATGCTTAACGATAACCTGCAACCGGAAGGGCCGAAAACGGCGTCGATTCCGATTAACAGTAAc                                                           >  1:3925854/1‑93 (MQ=255)
                                   gcgcAGTAGGCGTCTCCAGCGATGTGGTGATGCTTAACGATAACCTGCAACCGGAAGGGCCGAAAACGGCGTCGATTCCGATTAACAGTAAcc                                                          >  2:4661330/1‑93 (MQ=255)
                                   gcgcAGTAGGCGTCTCCAGCGATGTGGTGATGCTTAACGATAACCTGCAACCGGAAGGGCCGAAAACGGCGTCGATTCCGATTAACAGTAAcc                                                          <  2:7047261/93‑1 (MQ=255)
                                   gcgcAGTAGGCGTCTCCAGCGATGTGGTGATGCTTAACGATAACCTGCAACCGGAAGGGCCGAAAACGGCGTCGATTCCGATTAACAGTAAcc                                                          <  1:3394537/93‑1 (MQ=255)
                                    cgcAGTAGGCGTCTCCAGCGATGTGGTGATGCTTAACGATAACCTGCAACCGGAAGGGCCGAAAACGGCGTCGATTCCGATTAACAGTAACCt                                                         <  1:5273904/93‑1 (MQ=255)
                                       aGTAGGCGTCTCCAGCGATGTGGTGATGCTTAACGATAACCTGCAACCGGAAGGGCCGAAAACGGCGTCGATTCCGATTAACAGTAACCTGAt                                                      <  1:2271131/93‑1 (MQ=255)
                                        gTAGGCGTCTCCAGCGATGTGGTGATGCTTAACGATAACCTGCAACCGGAAGGGCCGAAAACGGCGTCGATTCCGATTAACAGTAACCTGAtt                                                     <  1:6278517/93‑1 (MQ=255)
                                         tAGGCGTCTCCAGCGATGTGGTGATGCTTAACGATAACCTGCAACCGGAAGGGCCGAAAACGGCGTCGATTCCGATTAACAGTAACCTGATTg                                                    >  1:1972142/1‑93 (MQ=255)
                                          aGGCGTCTCCAGCGATGTGGTGATGCTTAACGATAACCTGCAACCGGAAGGGCCGAAAACGGCGTCGATTCCGATTAACAGTAACCTGATTGt                                                   >  1:5208148/1‑93 (MQ=255)
                                           ggCGTCTCCAGCGATGTGGTGATGCTTAACGATAACCTGCAACCGGAAGGGCCGAAAACGGCGTCGATTCCGATTAACAGTAACCTGATTGTc                                                  <  2:1965254/93‑1 (MQ=255)
                                              gTCTCCAGCGATGTGGTGATGCTTAACGATAACCTGCAACCGGAAGGGCCGAAAACGGCGTCGATTCCGATTAACAGTAACCTGATTGTCCAc                                               <  2:951549/93‑1 (MQ=255)
                                              gTCTCCAGCGATGTGGTGATGCTTAACGATAACCTGCAACCGGAAGGGCCGAAAACGGCGTCGATTCCGATTAACAGTAACCTGATTGTCCAc                                               >  2:228619/1‑93 (MQ=255)
                                              gTCTCCAGCGATGTGGTGATGCTTAACGATAACCTGCAACCGGAAGGGCCGAAAACGGCGTCGATTCCGATTAACAGTAACCTGATTGTCCAc                                               >  2:6723849/1‑93 (MQ=255)
                                               tctcCAGCGATGTGGTGATGCTTAACGATAACCTGCAACCGGAAGGGCCGAAAACGGCGTCGATTCCGATTAACAGTAACCTGATTGTCCACg                                              >  2:7166367/1‑93 (MQ=255)
                                                ctcCAGCGATGTGGTGATGCTTAACGATAACCTGCAACCGGAAGGGCCGAAAACGGCGTCGATTCCGATTAACAGTAACCTGATTGTCCACGt                                             <  1:3725928/93‑1 (MQ=255)
                                                 tcCAGCGATGTGGTGATGCTTAACGATAACCTGCAACCGGAAGGGCCGAAAACGGCGTCGATTCCGATTAACAGTAACCTGATTGTCCACGtt                                            >  2:2212584/1‑93 (MQ=255)
                                                 tcCAGCGATGTGGTGATGCTTAACGATAACCTGCAACCGGAAGGGCCGAAAACGGCGTCGATTCCGATTAACAGTAACCTGATTGTCCACGtt                                            <  2:621223/93‑1 (MQ=255)
                                                 tcCAGCGATGTGGTGATGCTTAACGATAACCTGCAACCGGAAGGGCCGAAAACGGCGTCGATTCCGATTAACAGTAACCTGATTGTCCACGtt                                            <  2:4507359/93‑1 (MQ=255)
                                                   cAGCGATGTGGTGATGCTTAACGATAACCTGCAACCGGAAGGGCCGAAAACGGCGTCGATTCCGATTAACAGTAACCTGATTGTCCACGTTgg                                          >  2:2458618/1‑93 (MQ=255)
                                                   cAGCGATGTGGTGATGCTTAACGATAACCTGCAACCGGAAGGGCCGAAAACGGCGTCGATTCCGATTAACAGTAACCTGATTGTCCACGTTgg                                          <  1:5052623/93‑1 (MQ=255)
                                                   cAGCGATGTGGTGATGCTTAACGATAACCTGCAACCGGAAGAGCCGAAAACGGCGTCGATTCCGATTAACAGTAACCTGATTGTCCACGTTgg                                          >  1:6920741/1‑93 (MQ=255)
                                                    aGCGATGTGGTGATGCTTAACGATAACCTGCAACCGGAAGGGCCGAAAACGGCGTCGATTCCGATTAACAGTAACCTGATTGTCCACGTTGGc                                         >  2:3366585/1‑93 (MQ=255)
                                                    aGCGATGTGGTGATGCTTAACGATAACCTGCAACCGGAAGAGCCGAAAACGGCGTCGATTCCGATTAACAGTAACCTGATTGTCCACGTTGGc                                         >  1:4023554/1‑93 (MQ=255)
                                                     gCGATGTGGTGATGCTTAACGATAACCTGCAACCGGAAGGGCCGAAAACGGCGTCGATTCCGATTAACAGTAACCTGATTGTCCACGTTGGca                                        >  1:4384580/1‑93 (MQ=255)
                                                       gATGTGGTGATGCTTAACGATAACCTGCAACCGGAAGGGCCGAAAACGGCGTCGATTCCGATTAACAGTAACCTGATTGTCCACGTTGGcaac                                      >  1:4852641/1‑93 (MQ=255)
                                                         tgtgGTGATGCTTAACGATAACCTGCAACCGGAAGGGCCGAAAACGGCGTCGATTCCGATTAACAGTAACCTGATTGTCCACGTTGGcaacaa                                    >  1:2555872/1‑93 (MQ=255)
                                                         tgtgGTGATGCTTAACGATAACCTGCAACCGGAAGAGCCGAAAACGGCGTCGATTCCGATTAACAGTAACCTGATTGTCCACGTTGGcaacaa                                    <  2:6526593/93‑1 (MQ=255)
                                                          gtggtgATGCTTAACGATAACCTGCAACCGGAAGGGCCGAAAACGGCGTCGATTCCGATTAACAGTAACCTGATTGTCCACGTTGGCAACAAt                                   >  2:100363/1‑93 (MQ=255)
                                                          gtggtgATGCTTAACGATAACCTGCAACCGGAAGGGCCGAAAACGGCGTCGATTCCGATTAACAGTAACCTGATTGTCCACGTTGGCAACAAt                                   >  2:5113468/1‑93 (MQ=255)
                                                          gtggtgATGCTTAACGATAACCTGCAACCGGAAGAGCCGAAAACGGCGTCGATTCCGATTAACAGTAACCTGATTGTCCACGTTGGCAACAAt                                   <  2:5619093/93‑1 (MQ=255)
                                                           tggtgATGCTTAACGATAACCTGCAACCGGAAGGGCCGAAAACGGCGTCGATTCCGATTAACAGTAACCTGATTGTCCACGTTGGCAACAAtg                                  >  2:4850906/1‑93 (MQ=255)
                                                           tggtgATGCTTAACGATAACCTGCAACCGGAAGGGCCGAAAACGGCGTCGATTCCGATTAACAGTAACCTGATTGTCCACGTTGGCAACAAtg                                  >  2:131180/1‑93 (MQ=255)
                                                            ggtgATGCTTAACGATAACCTGCAACCGGAAGGGCCGAAAACGGCGTCGATTCCGATTAACAGTAACCTGATTGTCCACGTTGGCAACAAtgt                                 <  2:605318/93‑1 (MQ=255)
                                                             gtgATGCTTAACGATAACCTGCAACCGGAAGGGCCGAAAACGGCGTCGATTCCGATTAACAGTAACCTGATTGTCCACGTTGGCAACAAtgtg                                >  1:1389189/1‑93 (MQ=255)
                                                             gtgATGCTTAACGATAACCTGCAACCGGAAGGGCCGAAAACGGCGTCGATTCCGATTAACAGTAACCTGATTGTCCACGTTGGCAACAAtgtg                                >  2:1223877/1‑93 (MQ=255)
                                                             gtgATGCTTAACGATAACCTGCAACCGGAAGAGCCGAAAACGGCGTCGATTCCGATTAACAGTAACCTGATTGTCCACGTTGGCAACAAtgtg                                <  2:2725509/93‑1 (MQ=255)
                                                              ngATGCTTAACGATAACCTGCAACCGGAAGGGCCGAAAACGGCGTCGATTCCGATTAACAGTAACCTGATTGTCCACGTTGGCAACAATGTgc                               <  2:3435811/92‑1 (MQ=255)
                                                                 tGCTTAACGATAACCTGCAACCGGAAGGGCCGAAAACGGCGTCGATTCCGATTAACAGTAACCTGATTGTCCACGTTGGCAACAATGTGCGCa                            >  2:363673/1‑93 (MQ=255)
                                                                 tGCTTAACGATAACCTGCAACCGGAAGGGCCGAAAACGGCGTCGATTCCGATTAACAGTAACCTGATTGTCCACGTTGGCAACAATGTGCGCa                            <  2:808323/93‑1 (MQ=255)
                                                                 tGCTTAACGATAACCTGCAACCGGAAGGGCCGAAAACGGCGTCGATTCCGATTAACAGTAACCTGATTGTCCACGTTGGCAACAATGTGCGCa                            <  2:1908922/93‑1 (MQ=255)
                                                                 tGCTTAACGATAACCTGCAACCGGAAGGGCCGAAAACGGCGTCGATTCCGATTAACAGTAACCTGATTGTCCACGTTGGCAACAATGTGCGCa                            >  1:5999499/1‑93 (MQ=255)
                                                                  gCTTAACGATAACCTGCAACCGGAAGGGCCGAAAACGGCGTCGATTCCGATTAACAGTAACCTGATTGTCCACGTTGGCAACAATGTGCGCAt                           <  2:4272707/93‑1 (MQ=255)
                                                                  gCTTAACGATAACCTGCAACCGGAAGGGCCGAAAACGGCGTCGATTCCGATTAACAGTAACCTGATTGTCCACGTTGGCAACAATGTGCGCAt                           <  2:6689596/93‑1 (MQ=255)
                                                                   cTTAACGATAACCTGCAACCGGAAGGGCCGAAAACGGCGTCGATTCCGATTAACAGTAACCTGATTGTCCACGTTGGCAACAATGTGCGCAtt                          >  1:5486038/1‑93 (MQ=255)
                                                                    ttAACGATAACCTGCAACCGGAAGGGCCGAAAACGGCGTCGATTCCGATTAACAGTAACCTGATTGTCCACGTTGGCAACAATGTGCGCATTg                         >  2:5384390/1‑93 (MQ=255)
                                                                      aaCGATAACCTGCAACCGGAAGGGCCGAAAACGGCGTCGATTCCGATTAACAGTAACCTGATTGTCCACGTTGGCAACAATGTGCGCATTGAc                       <  1:6810628/93‑1 (MQ=255)
                                                                      aaCGATAACCTGCAACCGGAAGGGCCGAAAACGGCGTCGATTCCGATTAACAGTAACCTGATTGTCCACGTTGGCAACAATGTGCGCATTGAc                       <  2:6501777/93‑1 (MQ=255)
                                                                        cGATAACCTGCAACCGGAAGAGCCGAAAACGGCGTCGATTCCGATTAACAGTAACCTGATTGTCCACGTTGGCAACAATGTGCGCATTGACGc                     >  1:5644818/1‑93 (MQ=255)
                                                                         gATAACCTGCAACCGGAAGGGCCGAAAACGGCGTCGATTCCGATTAACAGTAACCTGATTGTCCACGTTGGCAACAATGTGCGCATTGACGcc                    <  2:582557/93‑1 (MQ=255)
                                                                         gATAACCTGCAACCGGAAGGGCCGAAAACGGCGTCGATTCCGATTAACAGTAACCTGATTGTCCACGTTGGCAACAATGTGCGCATTGACGcc                    <  2:6954708/93‑1 (MQ=255)
                                                                            aaCCTGCAACCGGAAGGGCCGAAAACGGCGTCGATTCCGATTAACAGTAACCTGATTGTCCACGTTGGCAACAATGTGCGCATTGACGCCttt                 <  2:3728619/93‑1 (MQ=255)
                                                                            aaCCTGCAACCGGAAGGGCCGAAAACGGCGTCGATTCCGATTAACAGTAACCTGATTGTCCACGTTGGCAACAATGTGCGCATTGACGCCttt                 <  2:2772562/93‑1 (MQ=255)
                                                                              ccTGCAACCGGAAGGGCCGAAAACGGCGTCGATTCCGATTAACAGTAACCTGATTGTCCACGTTGGCAACAATGTGCGCATTGACGCCTTTgg               >  1:6154007/1‑93 (MQ=255)
                                                                              ccTGCAACCGGAAGGGCCGAAAACGGCGTCGATTCCGATTAACAGTAACCTGATTGTCCACGTTGGCAACAATGTGCGCATTGACGCCTTTgg               >  1:7142559/1‑93 (MQ=255)
                                                                               cTGCAACCGGAAGGGCCGAAAACGGCGTCGATTCCGATTAACAGTAACCTGATTGTCCACGTTGGCAACAATGTGCGCATTGACGCCTTTGGc              <  1:6953497/93‑1 (MQ=255)
                                                                                tGCAACCGGAAGGGCCGAAAACGGCGTCGATTCCGATTAACAGTAACCTGATTGTCCACGTTGGCAACAATGTGCGCATTGACGCCTTTGGcc             >  1:1615552/1‑93 (MQ=255)
                                                                                tGCAACCGGAAGGGCCGAAAACGGCGTCGATTCCGATTAACAGTAACCTGATTGTCCACGTTGGCAACAATGTGCGCATTGACGCCTTTGGcc             <  2:2101660/93‑1 (MQ=255)
                                                                                 gCAACCGGAAGGGCCGAAAACGGCGTCGATTCCGATTAACAGTAACCTGATTGTCCACGTTGGCAACAATGTGCGCATTGACGCCTTTGGCCt            >  1:4124467/1‑93 (MQ=255)
                                                                                 gCAACCGGAAGGGCCGAAAACGGCGTCGATTCCGATTAACAGTAACCTGATTGTCCACGTTGGCAACAATGTGCGCATTGACGCCTTTGGCCt            >  1:125504/1‑93 (MQ=255)
                                                                                  cAACCGGAAGAGCCGAAAACGGCGTCGATTCCGATTAACAGTAACCTGATTGTCCACGTTGGCAACAATGTGCGCATTGACGCCTTTGGCCTg           >  1:1666818/1‑93 (MQ=255)
                                                                                   aaCCGGAAGAGCCGAAAACGGCGTCGATTCCGATTAACAGTAACCTGATTGTCCACGTTGGCAACAATGTGCGCATTGACGCCTTTGGCCTGa          <  2:7487777/93‑1 (MQ=255)
                                                                                    aCCGGAAGGGCCGAAAACGGCGTCGATTCCGATTAACAGTAACCTGATTGTCCACGTTGGCAACAATGTGCGCATTGACGCCTTTGGCCTGaa         >  2:5075899/1‑93 (MQ=255)
                                                                                    aCCGGAAGGGCCGAAAACGGCGTCGATTCCGATTAACAGTAACCTGATTGTCCACGTTGGCAACAATGTGCGCATTGACGCCTTTGGCCTGaa         <  2:887680/93‑1 (MQ=255)
                                                                                            |                                                                                           
TTGGGCGCGCATCGTGGTGCACGATCTGCCGGAAAGCGCAGTAGGCGTCTCCAGCGATGTGGTGATGCTTAACGATAACCTGCAACCGGAAGAGCCGAAAACGGCGTCGATTCCGATTAACAGTAACCTGATTGTCCACGTTGGCAACAATGTGCGCATTGACGCCTTTGGCCTGAAAGCGCGG  >  NC_000913/4444649‑4444832

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CTTGGGCGCGCATCGTGGTGCACGATCTGCCGGAAAGCGCAGTAGGCGTCTCCAGCGATGTGGTGATGCTTAACGATAACCTGCAACCGGAAGAGCCGAAAACGGCGTCGATTCCGATTAACAGTAACCTGATTGTCCACGTTGGCAACAATGTGCGCATTGACGCCTTTGGCCTGAAAGCGCGGCT  >  NC_000913/4444648‑4444834
                                                                                             |                                                                                             
CTTGGGCGCGCATCGTGGTGCACGATCTGCCGGAAAGCGCAGTAGGCGTCTCCAGCGATGTGGTGATGCTTAACGATAACCTGCAACCGGAAGG                                                                                               <  SRR4302542.6501035/94‑1 (MQ=60)
CTTGGGCGCGCATCGTGGTGCACGATCTGCCGGAAAGCGCAGTAGGCGTCTCCAGCGATGTGGTGATGCTTAACGATAACCTGCAACCGGAAGG                                                                                               <  SRR4302542.5897697/94‑1 (MQ=60)
CTTGGGCGCGCATCGTGGTGCACGATCTGCCGGAAAGCGCAGTAGGCGTCTCCAGCGATGTGGTGATGCTTAACGATAACCTGCAACCGGAAGA                                                                                               >  SRR4302542.5906483/1‑94 (MQ=60)
   GGGCGCGCATCGTGGTGCACGATCTGCCGGAAAGCGCAGTAGGCGTCTCCAGCGATGTGGTGATGCTTAACGATAACCTGCAACCGGAAGGGCC                                                                                            <  SRR4302542.3247688/94‑1 (MQ=60)
   GGGCGCGCATCGTGGTGCACGATCTGCCGGAAAGCGCAGTAGGCGTCTCCAGCGATGTGGTGATGCTTAACGATAACCTGCAACCGGAAGGGCC                                                                                            <  SRR4302542.6315213/94‑1 (MQ=60)
   GGGCGCGCATCGTGGTGCACGATCTGCCGGAAAGCGCAGTAGGCGTCTCCAGCGATGTGGTGATGCTTAACGATAACCTGCAACCGGAAGGGCC                                                                                            >  SRR4302542.2920753/1‑94 (MQ=60)
    GGCGCGCATCGTGGTGCACGATCTGCCGGAAAGCGCAGTAGGCGTCTCCAGCGATGTGGTGATGCTTAACGATAACCTGCAACCGGAAGGGCCG                                                                                           <  SRR4302542.256198/94‑1 (MQ=60)
    GGCGCGCATCGTGGTGCACGATCTGCCGGAAAGCGCAGTAGGCGTCTCCAGCGATGTGGTGATGCTTAACGATAACCTGCAACCGGAAGAGCCG                                                                                           <  SRR4302542.1005193/94‑1 (MQ=60)
      CGCGCATCGTGGTGCACGATCTGCCGGAAAGCGCAGTAGGCGTCTCCAGCGATGTGGTGATGCTTAACGATAACCTGCAACCGGAAGGGCCGAA                                                                                         >  SRR4302542.1465735/1‑94 (MQ=60)
      CGCGCATCGTGGTGCACGATCTGCCGGAAAGCGCAGTAGGCGTCTCCAGCGATGTGGTGATGCTTAACGATAACCTGCAACCGGAAGGGCCGAA                                                                                         >  SRR4302542.1577909/1‑94 (MQ=60)
      CGCGCATCGTGGTGCACGATCTGCCGGAAAGCGCAGTAGGCGTCTCCAGCGATGTGGTGATGCTTAACGATAACCTGCAACCGGAAGGGCCGAA                                                                                         <  SRR4302542.4065918/94‑1 (MQ=60)
        CGCATCGTGGTGCACGATCTGCCGGAAAGCGCAGTAGGCGTCTCCAGCGATGTGGTGATGCTTAACGATAACCTGCAACCGGAAGGGCCGAAAA                                                                                       <  SRR4302542.2993333/94‑1 (MQ=60)
         GCATCGTGGTGCACGATCTGCCGGAAAGCGCAGTAGGCGTCTCCAGCGATGTGGTGATGCTTAACGATAACCTGCAACCGGAAGGGCCGAAAAC                                                                                      <  SRR4302542.1418124/94‑1 (MQ=60)
         GCATCGTGGTGCACGATCTGCCGGAAAGCGCAGTAGGCGTCTCCAGCGATGTGGTGATGCTTAACGATAACCTGCAACCGGAAGGGCCGAAAAC                                                                                      <  SRR4302542.5640331/94‑1 (MQ=60)
         GCATCGTGGTGCACGATCTGCCGGAAAGCGCAGTAGGCGTCTCCAGCGATGTGGTGATGCTTAACGATAACCTGCAACCGGAAGGGCCGAAAAC                                                                                      <  SRR4302542.5375219/94‑1 (MQ=60)
         GCATCGTGGTGCACGATCTGCCGGAAAGCGCAGTAGGCGTCTCCAGCGATGTGGTGATGCTTAACGATAACCTGCAACCGGAAGAGCCGAAAAC                                                                                      <  SRR4302542.958658/94‑1 (MQ=60)
         GCATCGTGGTGCACGATCTGCCGGAAAGCGCAGTAGGCGTCTCCAGCGATGTGCTGATGCTTAACGATAACCTGCAACCGGAAGGGCCGAAAAC                                                                                      <  SRR4302542.6286006/94‑1 (MQ=60)
          CATCGTGGTGCACGATCTGCCGGAAAGCGCAGTAGGCGTCTCCAGCGATGTGGTGATGCTTAACGATAACCTGCAACCGGAAGGGCCGAAAACG                                                                                     <  SRR4302542.929926/94‑1 (MQ=60)
          CATCGTGGTGCACGATCTGCCGGAAAGCGCAGTAGGCGTCTCCAGCGATGTGGTGATGCTTAACGATAACCTGCAACCGGAAGGGCCGAAAACG                                                                                     <  SRR4302542.5187762/94‑1 (MQ=60)
           ATCGTGGTGCACGATCTGCCGGAAAGCGCAGTAGGCGTCTCCAGCGATGTGGTGATGCTTAACGATAACCTGCAACCGGAAGGGCCGAAAACGG                                                                                    >  SRR4302542.6493776/1‑94 (MQ=60)
            TCGTGGTGCACGATCTGCCGGAAAGCGCAGTAGGCGTCTCCAGCGATGTGGTGATGCTTAACGATAACCTGCAACCGGAAGGGCCGAAAACGGC                                                                                   <  SRR4302542.2696590/94‑1 (MQ=60)
             CGTGGTGCACGATCTGCCGGAAAGCGCAGTAGGCGTCTCCAGCGATGTGGTGATGCTTAACGATAACCTGCAACCGGAAGGGCCGAAAACGGCG                                                                                  >  SRR4302542.199977/1‑94 (MQ=60)
               TGGTGCACGATCTGCCGGAAAGCGCAGTAGGCGTCTCCAGCGATGTGGTGATGCTTAACGATAACCTGCAACCGGAAGGGCCGAAAACGGCGTC                                                                                <  SRR4302542.3089332/94‑1 (MQ=60)
               TGGTGCACGATCTGCCGGAAAGCGCAGTAGGCGTCTCCAGCGATGTGGTGATGCTTAACGATAACCTGCAACCGGAAGGGCCGAAAACGGCGTC                                                                                <  SRR4302542.513162/94‑1 (MQ=60)
                GGTGCACGATCTGCCGGAAAGCGCAGTAGGCGTCTCCAGCGATGTGGTGATGCTTAACGATAACCTGCAACCGGAAGGGCCGAAAACGGCGTCG                                                                               <  SRR4302542.6057902/94‑1 (MQ=60)
                GGTGCACGATCTGCCGGAAAGCGCAGTAGGCGTCTCCAGCGATGTGGTGATGCTTAACGATAACCTGCAACCGGAAGGGCCGAAAACGGCGTCG                                                                               <  SRR4302542.1277267/94‑1 (MQ=60)
                 GTGCACGATCTGCCGGAAAGCGCAGTAGGCGTCTCCAGCGATGTGGTGATGCTTAACGATAACCTGCAACCGGAAGGGCCGAAAACGGCGTCGA                                                                              >  SRR4302542.2686736/1‑94 (MQ=60)
                  TGCACGATCTGCCGGAAAGCGCAGTAGGCGTCTCCAGCGATGTGGTGATGCTTAACGATAACCTGCAACCGGAAGGGCCGAAAACGGCGTCGAT                                                                             <  SRR4302542.2758228/94‑1 (MQ=60)
                    CACGATCTGCCGGAAAGCGCAGTAGGCGTCTCCAGCGATGTGGTGATGCTTAACGATAACCTGCAACCGGAAGGGCCGAAAACGGCGTCGATTC                                                                           <  SRR4302542.264613/94‑1 (MQ=60)
                    CACGATCTGCCGGAAAGCGCAGTAGGCGTCTCCAGCGATGTGGTGATGCTTAACGATAACCTGCAACCGGAAGGGCCGAAAACGGCGTCGATTC                                                                           >  SRR4302542.909210/1‑94 (MQ=60)
                     ACGATCTGCCGGAAAGCGCAGTAGGCGTCTCCAGCGATGTGGTGATGCTTAACGATAACCTGCAACCGGAAGGGCCGAAAACGGCGTCGATTCC                                                                          >  SRR4302542.2921689/1‑94 (MQ=60)
                     ACGATCTGCCGGAAAGCGCAGTAGGCGTCTCCAGCGATGTGGTGATGCTTAACGATAACCTGCAACCGGAAGGGCCGAAAACGGCGTCGATTAC                                                                          <  SRR4302542.4571838/94‑1 (MQ=60)
                     ACGATCTGCCGGAAAGCGCAGTAGGCGTCTCCAGCGATGTGGTGATGCTTAACGATAACCTGCAACCGGAAGAGCCGAAAACGGCGTCGATTCC                                                                          <  SRR4302542.761694/94‑1 (MQ=60)
                      CGATCTGCCGGAAAGCGCAGTAGGCGTCTCCAGCGATGTGGTGATGCTTAACGATAACCTGCAACCGGAAGGGCCGAAAACGGCGTCGATTCCG                                                                         >  SRR4302542.4706589/1‑94 (MQ=60)
                       GATCTGCCGGAAAGCGCAGTAGGCGTCTCCAGCGATGTGGTGATGCTTAACGATAACCTGCAACCGGAAGGGCCGAAAACGGCGTCGATTCCGA                                                                        >  SRR4302542.3819954/1‑94 (MQ=60)
                       GATCTGCCGGAAAGCGCAGTAGGCGTCTCCAGCGATGTGGTGATGCTTAACGATAACCTGCAACCGGAAGAGCCGAAAACGGCGTCGATTCCGA                                                                        >  SRR4302542.5155848/1‑94 (MQ=60)
                       GATCTGCCGGAAAGCGCAGTAGGCGTCTCCAGCGATGTGGTGATGCTTAACGATAACCTGCAACCGGAAGAGCCGAAAACGGCGTCGATTCCGA                                                                        >  SRR4302542.590553/1‑94 (MQ=60)
                        ATCTGCCGGAAAGCGCAGTAGGCGTCTCCAGCGATGTGGTGATGCTTAACGATAACCTGCAACCGGAAGGGCCGAAAACGGCGTCGATTCCGAT                                                                       >  SRR4302542.209710/1‑94 (MQ=60)
                        ATCTGCCGGAAAGCGCAGTAGGCGTCTCCAGCGATGTGGTGATGCTTAACGATAACCTGCAACCGGAAGGGCCGAAAACGGCGTCGATTCCGAT                                                                       <  SRR4302542.3040495/94‑1 (MQ=60)
                        ATCTGCCGGAAAGCGCAGTAGGCGTCTCCAGCGATGTGGTGATGCTTAACGATAACCTGCAACCGGAAGGGCCGAAAACGGCGTCGATTCCGAT                                                                       <  SRR4302542.5977514/94‑1 (MQ=60)
                         TCTGCCGGAAAGCGCAGTAGGCGTCTCCAGCGATGTGGTGATGCTTAACGATAACCTGCAACCGGAAGGGCCGAAAACGGCGTCGATTCCGATT                                                                      >  SRR4302542.1758335/1‑94 (MQ=60)
                         TCTGCCGGAAAGCGCAGTAGGCGTCTCCAGCGATGTGGTGATGCTTAACGATAACCTGCAACCGGAAGGGCCGAAAACGGCGTCGATTCCGATT                                                                      <  SRR4302542.3520007/94‑1 (MQ=60)
                          CTGCCGGAAAGCGCAGTAGGCGTCTCCAGCGATGTGGTGATGCTTAACGATAACCTGCAACCGGAAGGGCCGAAAACGGCGTCGATTCCGATTA                                                                     >  SRR4302542.1824986/1‑94 (MQ=60)
                           TGCCGGAAAGCGCAGTAGGCGTCTCCAGCGATGTGGTGATGCTTAACGATAACCTGCAACCGGAAGGGCCGAAAACGGCGTCGATTCCGATTAA                                                                    <  SRR4302542.3493824/94‑1 (MQ=60)
                           TGCCGGAAAGCGCAGTAGGCGTCTCCAGCGATGTGGTGATGCTTAACGATAACCTGCAACCGGAAGGGCCGAAAACGGCGTCGATTCCGATTAA                                                                    >  SRR4302542.4973797/1‑94 (MQ=60)
                            GCCGGAAAGCGCAGTAGGCGTCTCCAGCGATGTGGTGATGCTTAACGATAACCTGCAACCGGAAGGGCCGAAAACGGCGTCGATTCCGATTAAC                                                                   <  SRR4302542.3992717/94‑1 (MQ=60)
                             CCGGAAAGCGCAGTAGGCGTCTCCAGCGATGTGGTGATGCTTAACGATAACCTGCAACCGGAAGGGCCGAAAACGGCGTCGATTCCGATTAACA                                                                  >  SRR4302542.5544277/1‑94 (MQ=60)
                              CGGAAAGCGCAGTAGGCGTCTCCAGCGATGTGGTGATGCTTAACGATAACCTGCAACCGGAAGGGCCGAAAACGGCGTCGATTCCGATTAACAG                                                                 <  SRR4302542.2729840/94‑1 (MQ=60)
                              CGGAAAGCGCAGTAGGCGTCTCCAGCGATGTGGTGATGCTTAACGATAACCTGCAACCGGAAGGGCCGAAAACGGCGTCGATTCCGATTAACAG                                                                 <  SRR4302542.2971873/94‑1 (MQ=60)
                              CGGAAAGCGCAGTAGGCGTCTCCAGCGATGTGGTGATGCTTAACGATAACCTGCAACCGGAAGAGCCGAAAACGGCGTCGATTCCGATTAACAG                                                                 >  SRR4302542.796444/1‑94 (MQ=60)
                               GGAAAGCGCAGTAGGCGTCTCCAGCGATGTGGTGATGCTTAACGATAACCTGCAACCGGAAGGGCCGAAAACGGCGTCGATTCCGATTAACAGT                                                                <  SRR4302542.1094242/94‑1 (MQ=60)
                                  AAGCGCAGTAGGCGTCTCCAGCGATGTGGTGATGCTTAACGATAACCTGCAACCGGAAGGGCCGAAAACGGCGTCGATTCCGATTAACAGTAAC                                                             >  SRR4302542.3964591/1‑94 (MQ=60)
                                  AAGCGCAGTAGGCGTCTCCAGCGATGTGGTGATGCTTAACGATAACCTGCAACCGGAAGGGCCGAAAACGGCGTCGATTCCGATTAACAGTAAC                                                             >  SRR4302542.3964589/1‑94 (MQ=60)
                                   AGCGCAGTAGGCGTCTCCAGCGATGTGGTGATGCTTAACGATAACCTGCAACCGGAAGGGCCGAAAACGGCGTCGATTCCGATTAACAGTAACC                                                            >  SRR4302542.7004632/1‑94 (MQ=60)
                                   AGCGCAGTAGGCGTCTCCAGCGATGTGGTGATGCTTAACGATAACCTGCAACCGGAAGGGCCGAAAACGGCGTCGATTCCGATTAACAGTAACC                                                            <  SRR4302542.2573635/94‑1 (MQ=60)
                                   AGCGCAGTAGGCGTCTCCAGCGATGTGGTGATGCTTAACGATAACCTGCAACCGGAAGAGCCGAAAACGGCGTCGATTCCGATTAACAGTAACC                                                            <  SRR4302542.6663162/94‑1 (MQ=60)
                                    GCGCAGTAGGCGTCTCCAGCGATGTGGTGATGCTTAACGATAACCTGCAACCGGAAGGGCCGAAAACGGCGTCGATTCCGATTAACAGTAACCT                                                           <  SRR4302542.3426385/94‑1 (MQ=60)
                                    GCGCAGTAGGCGTCTCCAGCGATGTGGTGATGCTTAACGATAACCTGCAACCGGAAGGGCCGAAAACGGCGTCGATTCCGATTAACAGTAACCT                                                           <  SRR4302542.6897094/94‑1 (MQ=60)
                                     CGCAGTAGGCGTCTCCAGCGATGTGGTGATGCTTAACGATAACCTGCAACCGGAAGGGCCGAAAACGGCGTCGATTCCGATTAACAGTAACCTG                                                          <  SRR4302542.5325151/94‑1 (MQ=60)
                                      GCAGTAGGCGTCTCCAGCGATGTGGTGATGCTTAACGATAACCTGCAACCGGAAGGGCCGAAAACGGCGTCGATTCCGATTAACAGTAACCTGA                                                         >  SRR4302542.5278794/1‑94 (MQ=60)
                                       CAGTAGGCGTCTCCAGCGATGTGGTGATGCTTAACGATAACCTGCAACCGGAAGGGCCGAAAACGGCGTCGATTCCGATTAACAGTAACCTGAT                                                        <  SRR4302542.2577598/94‑1 (MQ=60)
                                        AGTAGGCGTCTCCAGCGATGTGGTGATGCTTAACGATAACCTGCAACCGGAAGGGCCGAAAACGGCGTCGATTCCGATTAACAGTAACCTGATT                                                       <  SRR4302542.2290660/94‑1 (MQ=60)
                                         GTAGGCGTCTCCAGCGATGTGGTGATGCTTAACGATAACCTGCAACCGGAAGGGCCGAAAACGGCGTCGATTCCGATTAACAGTAACCTGATTG                                                      >  SRR4302542.1989182/1‑94 (MQ=60)
                                         GTAGGCGTCTCCAGCGATGTGGTGATGCTTAACGATAACCTGCAACCGGAAGGGCCGAAAACGGCGTCGATTCCGATTAACAGTAACCTGATTG                                                      <  SRR4302542.1814219/94‑1 (MQ=60)
                                         GTAGGCGTCTCCAGCGATGTGGTGATGCTTAACGATAACCTGCAACCGGAAGGGCCGAAAACGGCGTCGATTCCGATTAACAGTAACCTGATTG                                                      <  SRR4302542.6340392/94‑1 (MQ=60)
                                          TAGGCGTCTCCAGCGATGTGGTGATGCTTAACGATAACCTGCAACCGGAAGGGCCGAAAACGGCGTCGATTCCGATTAACAGTAACCTGATTGT                                                     >  SRR4302542.5258801/1‑94 (MQ=60)
                                           AGGCGTCTCCAGCGATGTGGTGATGCTTAACGATAACCTGCAACCGGAAGGGCCGAAAACGGCGTCGATTCCGATTAACAGTAACCTGATTGTC                                                    >  SRR4302542.4013550/1‑94 (MQ=60)
                                           AGGCGTCTCCAGCGATGTGGTGATGCTTAACGATAACCTGCAACCGGAAGGGCCGAAAACGGCGTCGATTCCGATTAACAGTAACCTGATTGTC                                                    >  SRR4302542.2484109/1‑94 (MQ=60)
                                           AGGCGTCTCCAGCGATGTGGTGATGCTTAACGATAACCTGCAACCGGAAGAGCCGAAAACGGCGTCGATTCCGATTAACAGTAACCTGATTGTC                                                    >  SRR4302542.5005375/1‑94 (MQ=60)
                                            GGCGTCTCCAGCGATGTGGTGATGCTTAACGATAACCTGCAACCGGAAGGGCCGAAAACGGCGTCGATTCCGATTAACAGTAACCTGATTGTCC                                                   >  SRR4302542.5688446/1‑94 (MQ=60)
                                             GCGTCTCCAGCGATGTGGTGATGCTTAACGATAACCTGCAACCGGAAGGGCCGAAAACGGCGTCGATTCCGATTAACAGTAACCTGATTGTCCA                                                  <  SRR4302542.1596238/94‑1 (MQ=60)
                                              CGTCTCCAGCGATGTGGTGATGCTTAACGATAACCTGCAACCGGAAGGGCCGAAAACGGCGTCGATTCCGATTAACAGTAACCTGATTGTCCAC                                                 >  SRR4302542.5651342/1‑94 (MQ=60)
                                               GTCTCCAGCGATGTGGTGATGCTTAACGATAACCTGCAACCGGAAGGGCCGAAAACGGCGTCGATTCCGATTAACAGTAACCTGATTGTCCACG                                                <  SRR4302542.1753939/94‑1 (MQ=60)
                                                 CTCCAGCGATGTGGTGATGCTTAACGATAACCTGCAACCGGAAGGGCCGAAAACGGCGTCGATTCCGATTAACAGTAACCTGATTGTCCACGTT                                              >  SRR4302542.2290248/1‑94 (MQ=60)
                                                 CTCCAGCGATGTGGTGATGCTTAACGATAACCTGCAACCGGAAGGGCCGAAAACGGCGTCGATTCCGATTAACAGTAACCTGATTGTCCACGTT                                              <  SRR4302542.3762823/94‑1 (MQ=60)
                                                 CTCCAGCGATGTGGTGATGCTTAACGATAACCTGCAACCGGAAGGGCCGAAAACGGCGTCGATTCCGATTAACAGTAACCTGATTGTCCACGTT                                              >  SRR4302542.4947902/1‑94 (MQ=60)
                                                 CTCCAGCGATGTGGTGATGCTTAACGATAACCTGCAACCGGAAGGGCCGAAAACGGCGTCGATTCCGATTAACAGTAACCTGATTGTCCACGTT                                              >  SRR4302542.3804558/1‑94 (MQ=60)
                                                  TCCAGCGATGTGGTGATGCTTAACGATAACCTGCAACCGGAAGGGCCGAAAACGGCGTCGATTCCGATTAACAGTAACCTGATTGTCCACGTTG                                             <  SRR4302542.2744101/94‑1 (MQ=60)
                                                   CCAGCGATGTGGTGATGCTTAACGATAACCTGCAACCGGAAGAGCCGAAAACGGCGTCGATTCCGATTAACAGTAACCTGATTGTCCACGTTGG                                            >  SRR4302542.6988688/1‑94 (MQ=60)
                                                    CAGCGATGTGGTGATGCTTAACGATAACCTGCAACCGGAAGGGCCGAAAACGGCGTCGATTCCGATTAACAGTAACCTGATTGTCCACGTTGGC                                           <  SRR4302542.5101684/94‑1 (MQ=60)
                                                    CAGCGATGTGGTGATGCTTAACGATAACCTGCAACCGGAAGAGCCGAAAACGGCGTCGATTCCGATTAACAGTAACCTGATTGTCCACGTTGGC                                           >  SRR4302542.4063250/1‑94 (MQ=60)
                                                     AGCGATGTGGTGATGCTTAACGATAACCTGCAACCGGAAGGGCCGAAAACGGCGTCGATTCCGATTAACAGTAACCTGATTGTCCACGTTGGCA                                          >  SRR4302542.4427603/1‑94 (MQ=60)
                                                      GCGATGTGGTGATGCTTAACGATAACCTGCAACCGGAAGGGCCGAAAACGGCGTCGATTCCGATTAACAGTAACCTGATTGTCCACGTTGGCAA                                         <  SRR4302542.5113325/94‑1 (MQ=60)
                                                       CGATGTGGTGATGCTTAACGATAACCTGCAACCGGAAGGGCCGAAAACGGCGTCGATTCCGATTAACAGTAACCTGATTGTCCACGTTGGCAAC                                        >  SRR4302542.4899897/1‑94 (MQ=60)
                                                        GATGTGGTGATGCTTAACGATAACCTGCAACCGGAAGGGCCGAAAACGGCGTCGATTCCGATTAACAGTAACCTGATTGTCCACGTTGGCAACA                                       >  SRR4302542.4844962/1‑94 (MQ=60)
                                                         ATGTGGTGATGCTTAACGATAACCTGCAACCGGAAGGGCCGAAAACGGCGTCGATTCCGATTAACAGTAACCTGATTGTCCACGTTGGCAACAA                                      >  SRR4302542.2580653/1‑94 (MQ=60)
                                                           GTGGTGATGCTTAACGATAACCTGCAACCGGAAGGGCCGAAAACGGCGTCGATTCCGATTAACAGTAACCTGATTGTCCACGTTGGCAACAATG                                    <  SRR4302542.2775253/94‑1 (MQ=60)
                                                           GTGGTGATGCTTAACGATAACCTGCAACCGGAAGGGCCGAAAACGGCGTCGATTCCGATTAACAGTAACCTGATTGTCCACGTTGGCAACAATG                                    <  SRR4302542.6390487/94‑1 (MQ=60)
                                                            TGGTGATGCTTAACGATAACCTGCAACCGGAAGGGCCGAAAACGGCGTCGATTCCGATTAACAGTAACCTGATTGTCCACGTTGGCAACAATGT                                   <  SRR4302542.4864395/94‑1 (MQ=60)
                                                            TGGTGATGCTTAACGATAACCTGCAACCGGAAGGGCCGAAAACGGCGTCGATTCCGATTAACAGTAACCTGATTGTCCACGTTGGCAACAATGT                                   >  SRR4302542.7266211/1‑94 (MQ=60)
                                                             GGTGATGCTTAACGATAACCTGCAACCGGAAGGGCCGAAAACGGCGTCGATTCCGATTAACAGTAACCTGATTGTCCACGTTGGCAACAATGTG                                  >  SRR4302542.1401183/1‑94 (MQ=60)
                                                             GGTGATGCTTAACGATAACCTGCAACCGGAAGGGCCGAAAACGGCGTCGATTCCGATTAACAGTAACCTGATTGTCCACGTTGGCAACAATGTG                                  <  SRR4302542.1461679/94‑1 (MQ=60)
                                                                 ATGCTTAACGATAACCTGCAACCGGAAGGGCCGAAAACGGCGTCGATTCCGATTAACAGTAACCTGATTGTCCACGTTGGCAACAATGTGCGCA                              >  SRR4302542.5148446/1‑94 (MQ=60)
                                                                 ATGCTTAACGATAACCTGCAACCGGAAGGGCCGAAAACGGCGTCGATTCCGATTAACAGTAACCTGATTGTCCACGTTGGCAACAATGTGCGCA                              >  SRR4302542.6057606/1‑94 (MQ=60)
                                                                   GCTTAACGATAACCTGCAACCGGAAGGGCCGAAAACGGCGTCGATTCCGATTAACAGTAACCTGATTGTCCACGTTGGCAACAATGTGCGCATT                            >  SRR4302542.5539330/1‑94 (MQ=60)
                                                                    CTTAACGATAACCTGCAACCGGAAGGGCCGAAAACGGCGTCGATTCCGATTAACAGTAACCTGATTGTCCACGTTGGCAACAATGTGCGCATTG                           <  SRR4302542.930952/94‑1 (MQ=60)
                                                                     TTAACGATAACCTGCAACCGGAAGGGCCGAAAACGGCGTCGATTCCGATTAACAGTAACCTGATTGTCCACGTTGGCAACAATGTGCGCATTGA                          <  SRR4302542.1646685/94‑1 (MQ=60)
                                                                      TAACGATAACCTGCAACCGGAAGGGCCGAAAACGGCGTCGATTCCGATTAACAGTAACCTGATTGTCCACGTTGGCAACAATGTGCGCATTGAC                         <  SRR4302542.5890154/94‑1 (MQ=60)
                                                                      TAACGATAACCTGCAACCGGAAGAGCCGAAAACGGCGTCGATTCCGATTAACAGTAACCTGATTGTCCACGTTGGCAACAATGTGCGCATTGAC                         <  SRR4302542.510461/94‑1 (MQ=60)
                                                                      TAACGATAACCTGCAACCGGAAGAGCCGAAAACGGCGTCGATTCCGATTAACAGTAACCTGATTGTCCACGTTGGCAACAATGTGCGCATTGAC                         >  SRR4302542.1754567/1‑94 (MQ=60)
                                                                       AACGATAACCTGCAACCGGAAGGGCCGAAAACGGCGTCGATTCCGATTAACAGTAACCTGATTGTCCACGTTGGCAACAATGTGCGCATTGACG                        <  SRR4302542.6877584/94‑1 (MQ=60)
                                                                       AACGATAACCTGCAACCGGAAGGGCCGAAAACGGCGTCGATTCCGATTAACAGTAACCTGATTGTCCACGTTGGCAACAATGTGCGCATTGACG                        <  SRR4302542.63992/94‑1 (MQ=60)
                                                                       AACGATAACCTGCAACCGGAAGGGCCGAAAACGGCGTCGATTCCGATTAACAGTAACCTGATTGTCCACGTTGGCAACAATGTGCGCATTGACG                        <  SRR4302542.1165045/94‑1 (MQ=60)
                                                                       AACGATAACCTGCAACCGGAAGGGCCGAAAACGGCGTCGATTCCGATTAACAGTAACCTGATTGTCCACGTTGGCAACAATGTGCGCATTGACG                        <  SRR4302542.3442694/94‑1 (MQ=60)
                                                                       AACGATAACCTGCAACCGGAAGGGCCGAAAACGGCGTCGATTCCGATTAACAGTAACCTGATTGTCCACGTTGGCAACAATGTGCGCATTGACG                        >  SRR4302542.820211/1‑94 (MQ=60)
                                                                        ACGATAACCTGCAACCGGAAGAGCCGAAAACGGCGTCGATTCCGATTAACAGTAACCTGATTGTCCACGTTGGCAACAATGTGCGCATTGACGC                       >  SRR4302542.5699633/1‑94 (MQ=60)
                                                                         CGATAACCTGCAACCGGAAGGGCCGAAAACGGCGTCGATTCCGATTAACAGTAACCTGATTGTCCACGTTGGCAACAATGTGCGCATTGACGCC                      <  SRR4302542.2201221/94‑1 (MQ=60)
                                                                         CGATAACCTGCAACCGGAAGGGCCGAAAACGGCGTCGATTCCGATTAACAGTAACCTGATTGTCCACGTTGGCAACAATGTGCGCATTGACGCC                      >  SRR4302542.3794120/1‑94 (MQ=60)
                                                                         CGATAACCTGCAACCGGAAGGGCCGAAAACGGCGTCGATTCCGATTAACAGTAACCTGATTGTCCACGTTGGCAACAATGTGCGCATTGACGCC                      >  SRR4302542.7498653/1‑94 (MQ=60)
                                                                         CGATAACCTGCAACCGGAAGGGCCGAAAACGGCGTCGATTCCGATTAACAGTAACCTGATTGTCCACGTTGGCAACAATGTGCGCATTGACGCC                      >  SRR4302542.543772/1‑94 (MQ=60)
                                                                          GATAACCTGCAACCGGAAGAGCCGAAAACGGCGTCGATTCCGATTAACAGTAACCTGATTGTCCACGTTGGCAACAATGTGCGCATTGACGCCT                     <  SRR4302542.7132206/94‑1 (MQ=60)
                                                                            TAACCTGCAACCGGAAGGGCCGAAAACGGCGTCGATTCCGATTAACAGTAACCTGATTGTCCACGTTGGCAACAATGTGCGCATTGACGCCTTT                   <  SRR4302542.4980593/94‑1 (MQ=60)
                                                                             AACCTGCAACCGGAAGGGCCGAAAACGGCGTCGATTCCGATTAACAGTAACCTGATTGTCCACGTTGGCAACAATGTGCGCATTGACGCCTTTG                  <  SRR4302542.6702775/94‑1 (MQ=60)
                                                                             AACCTGCAACCGGAAGGGCCGAAAACGGCGTCGATTCCGATTAACAGTAACCTGATTGTCCACGTTGGCAACAATGTGCGCATTGACGCCTTTG                  <  SRR4302542.4139807/94‑1 (MQ=60)
                                                                              ACCTGCAACCGGAAGGGCCGAAAACGGCGTCGATTCCGATTAACAGTAACCTGATTGTCCACGTTGGCAACAATGTGCGCATTGACGCCTTTGG                 >  SRR4302542.7212648/1‑94 (MQ=60)
                                                                              ACCTGCAACCGGAAGGGCCGAAAACGGCGTCGATTCCGATTAACAGTAACCTGATTGTCCACGTTGGCAACAATGTGCGCATTGACGCCTTTGG                 >  SRR4302542.6213591/1‑94 (MQ=60)
                                                                               CCTGCAACCGGAAGGGCCGAAAACGGCGTCGATTCCGATTAACAGTAACCTGATTGTCCACGTTGGCAACAATGTGCGCATTGACGCCTTTGGC                >  SRR4302542.6021201/1‑94 (MQ=60)
                                                                               CCTGCAACCGGAAGGGCCGAAAACGGCGTCGATTCCGATTAACAGTAACCTGATTGTCCACGTTGGCAACAATGTGCGCATTGACGCCTTTGGC                >  SRR4302542.3222721/1‑94 (MQ=60)
                                                                                CTGCAACCGGAAGGGCCGAAAACGGCGTCGATTCCGATTAACAGTAACCTGATTGTCCACGTTGGCAACAATGTGCGCATTGACGCCTTTGGCC               >  SRR4302542.1629507/1‑94 (MQ=60)
                                                                                CTGCAACCGGAAGGGCCGAAAACGGCGTCGATTCCGATTAACAGTAACCTGATTGTCCACGTTGGCAACAATGTGCGCATTGACGCCTTTGGCC               >  SRR4302542.1639786/1‑94 (MQ=60)
                                                                                CTGCAACCGGAAGGGCCGAAAACGGCGTCGATTCCGATTAACAGTAACCTGATTGTCCACGTTGGCAACAATGTGCGCATTGACGCCTTTGGCC               <  SRR4302542.7021821/94‑1 (MQ=60)
                                                                                CTGCAACCGGAAGGGCCGAAAACGGCGTCGATTCCGATTAACAGTAACCTGATTGTCCACGTTGGCAACAATGTGCGCATTGACGCCTTTGGCC               >  SRR4302542.3618565/1‑94 (MQ=60)
                                                                                CTGCAACCGGAAGGGCCGAAAACGGCGTCGATTCCGATTAACAGTAACCTGATTGTCCACGTTGGCAACAATGTGCGCATTGACGCCTTTGGCC               >  SRR4302542.7013258/1‑94 (MQ=60)
                                                                                 TGCAACCGGAAGGGCCGAAAACGGCGTCGATTCCGATTAACAGTAACCTGATTGTCCACGTTGGCAACAATGTGCGCATTGACGCCTTTGGCCT              >  SRR4302542.4165096/1‑94 (MQ=60)
                                                                                 TGCAACCGGAAGGGCCGAAAACGGCGTCGATTCCGATTAACAGTAACCTGATTGTCCACGTTGGCAACAATGTGCGCATTGACGCCTTTGGCCT              >  SRR4302542.126529/1‑94 (MQ=60)
                                                                                 TGCAACCGGAAGGGCCGAAAACGGCGTCGATTCCGATTAACAGTAACCTGATTGTCCACGTTGGCAACAATGTGCGCATTGACGCCTTTGGCCT              <  SRR4302542.528943/94‑1 (MQ=60)
                                                                                  GCAACCGGAAGAGCCGAAAACGGCGTCGATTCCGATTAACAGTAACCTGATTGTCCACGTTGGCAACAATGTGCGCATTGACGCCTTTGGCCTG             >  SRR4302542.1681211/1‑94 (MQ=60)
                                                                                   CAACCGGAAGGGCCGAAAACGGCGTCGATTCCGATTAACAGTAACCTGATTGTCCACGTTGGCAACAATGTGCGCATTGACGCCTTTGGCCTGA            >  SRR4302542.965031/1‑94 (MQ=60)
                                                                                   CAACCGGAAGGGCCGAAAACGGCGTCGATTCCGATTAACAGTAACCTGATTGTCCACGTTGGCAACAATGTGCGCATTGACGCCTTTGGCCTGA            <  SRR4302542.5243133/94‑1 (MQ=60)
                                                                                   CAACCGGAAGGGCCGAAAACGGCGTCGATTCCGATTAACAGTAACCTGATTGTCCACGTTGGCAACAATGTGCGCATTGACGCCTTTGGCCTGA            >  SRR4302542.3267892/1‑94 (MQ=60)
                                                                                    AACCGGAAGAGCCGAAAACGGCGTCGATTCCGATTAACAGTAACCTGATTGTCCACGTTGGCAACAATGTGCGCATTGACGCCTTTGGCCTGAA           >  SRR4302542.5202799/1‑94 (MQ=60)
                                                                                     ACCGGAAGGGCCGAAAACGGCGTCGATTCCGATTAACAGTAACCTGATTGTCCACGTTGGCAACAATGTGCGCATTGACGCCTTTGGCCTGAAA          <  SRR4302542.6205343/94‑1 (MQ=60)
                                                                                     ACCGGAAGGGCCGAAAACGGCGTCGATTCCGATTAACAGTAACCTGATTGTCCACGTTGGCAACAATGTGCGCATTGACGCCTTTGGCCTGAAA          >  SRR4302542.3964039/1‑94 (MQ=60)
                                                                                      CCGGAAGGGCCGAAAACGGCGTCGATTCCGATTAACAGTAACCTGATTGTCCACGTTGGCAACAATGTGCGCATTGACGCCTTTGGCCTGAAAG         <  SRR4302542.1359139/94‑1 (MQ=60)
                                                                                        GGAAGGGCCGAAAACGGCGTCGATTCCGATTAACAGTAACCTGATTGTCCACGTTGGCAACAATGTGCGCATTGACGCCTTTGGCCTGAAAGCG       >  SRR4302542.4924657/1‑94 (MQ=60)
                                                                                        GGAAGGGCCGAAAACGGCGTCGATTCCGATTAACAGTAACCTGATTGTCCACGTTGGCAACAATGTGCGCATTGACGCCTTTGGCCTGAAAGCG       >  SRR4302542.4519591/1‑94 (MQ=60)
                                                                                         GAAGGGCCGAAAACGGCGTCGATTCCGATTAACAGTAACCTGATTGTCCACGTTGACAACAATGTGCGCATTGACGCCTTTGGCCTGAAAGCGC      <  SRR4302542.6970346/94‑1 (MQ=60)
                                                                                         GAAGAGCCGAAAACGGCGTCGATTCCGATTAACAGTAACCTGATTGTCCACGTTGGCAACAATGTGCGCATTGACGCCTTTGGCCTGAAAGCGC      >  SRR4302542.3610324/1‑94 (MQ=60)
                                                                                          AAGGGCCGAAAACGGCGTCGATTCCGATTAACAGTAACCTGATTGTCCACGTTGGCAACAATGTGCGCATTGACGCCTTTGGCCTGAAAGCGCG     <  SRR4302542.2038080/94‑1 (MQ=60)
                                                                                           AGGGCCGAAAACGGCGTCGATTCCGATTAACAGTAACCTGATTGTCCACGTTGGCAACAATGTGCGCATTGACGCCTTTGGCCTGAAAGCGCGG    <  SRR4302542.2556511/94‑1 (MQ=60)
                                                                                           AGGGCCGAAAACGGCGTCGATTCCGATTAACAGTAACCTGATTGTCCACGTTGGCAACAATGTGCGCATTGACGCCTTTGGCCTGAAAGCGCGG    >  SRR4302542.5318920/1‑94 (MQ=60)
                                                                                           AGAGCCGAAAACGGCGTCGATTCCGATTAACAGTAACCTGATTGTCCACGTTGGCAACAATGTGCGCATTGACGCCTTTGGCCTGAAAGCGCGG    <  SRR4302542.7119750/94‑1 (MQ=60)
                                                                                            GAGCCGAAAACGGCGTCGATTCCGATTAACAGTAACCTGATTGTCCACGTTGGCAACAATGTGCGCATTGACGCCTTTGGCCTGAAAGCGTGGC   <  SRR4302542.4334423/94‑1 (MQ=60)
                                                                                            GAGCCGAAAACGGCGTCGATTCCGATTAACAGTAACCTGATTGTCCACGTTGGCAACAATGTGCGCATTGACGCCTTTGGCCTGAAAGCGCGGC   <  SRR4302542.4334424/94‑1 (MQ=60)
                                                                                             GGCCGAAAACGGCGTCGATTCCGATTAACAGTAACCTGATTGTCCACGTTGGCAACAATGTGCGCATTGACGCCTTTGGCCTGAAAGCGCGGCT  <  SRR4302542.3146803/94‑1 (MQ=60)
                                                                                             GGCCGAAAACGGCGTCGATTCCGATTAACAGTAACCTGATTGTCCACGTTGGCAACAATGTGCGCATTGACGCCTTTGGCCTGAAAGCGCGGCT  <  SRR4302542.3146802/94‑1 (MQ=60)
                                                                                             GGCCGAAAACGGCGTCGATTCCGATTAACAGTAACCTGATTGTCCACGTTGGCAACAATGTGCGCATTGACGCCTTTGGCCTGAAAGCGCGGCT  <  SRR4302542.2326294/94‑1 (MQ=60)
                                                                                             |                                                                                             
CTTGGGCGCGCATCGTGGTGCACGATCTGCCGGAAAGCGCAGTAGGCGTCTCCAGCGATGTGGTGATGCTTAACGATAACCTGCAACCGGAAGAGCCGAAAACGGCGTCGATTCCGATTAACAGTAACCTGATTGTCCACGTTGGCAACAATGTGCGCATTGACGCCTTTGGCCTGAAAGCGCGGCT  >  NC_000913/4444648‑4444834

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑