Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1061 294.1 15065038 98.6% 14854127 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,476,406 A→G D124G (GAC→GGC)  yjgD → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,476,4060AG80.8% 521.2 / 131.6 260D124G (GAC→GGC) yjgDhypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (29/21);  new base G (98/112);  total (127/133)
Fisher's exact test for biased strand distribution p-value = 1.60e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01

Only 100 of 270 total aligned reads displayed.

GTTGAACAACTGATGACGCTGGCAGAGAAATTTGACGTTGAGTACGACGGATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGCGACGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCACTAATTAAGCGTAGCGCATCAGGCCCAGGTAGTTCCTGCAACCTGGTGA  >  NC_000913/4476315‑4476497
                                                                                           |                                                                                           
   gAACAACTGATGACGCTGGCAGAGAAATTTGACGTTGAGTACGACGGATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGCGGCGAt                                                                                         >  1:1267777/1‑93 (MQ=255)
   gAACAACTGATGACGCTGGCAGAGAAATTTGACGTTGAGTACGACGGATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGCGGCGAt                                                                                         >  2:6375850/1‑93 (MQ=255)
   gAACAACTGATGACGCTGGCAGAGAAATTTGACGTTGAGTACGACGGATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGCGGCGAt                                                                                         >  2:4697397/1‑93 (MQ=255)
    aacaacTGATGACGCTGGCAGAGAAATTTGACGTTGAGTACGACGGATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGCGACGATg                                                                                        <  1:6973917/93‑1 (MQ=255)
     acaacTGATGACGCTGGCAGAGAAATTTGACGTTGAGTACGACGGATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGCGGCGATGa                                                                                       >  2:1033050/1‑93 (MQ=255)
     acaacTGATGACGCTGGCAGAGAAATTTGACGTTGAGTACGACGGATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGCGGCGATGa                                                                                       >  2:4188849/1‑93 (MQ=255)
     acaacTGATGACGCTGGCAGAGAAATTTGACGTTGAGTACGACGGATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGCGGCGATGa                                                                                       <  1:2207806/93‑1 (MQ=255)
     acaacTGATGACGCTGGCAGAGAAATTTGACGTTGAGTACGACGGATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGCGGCGATGa                                                                                       <  1:2051304/93‑1 (MQ=255)
      caacTGATGACGCTGGCAGAGAAATTTGACGTTGAGTACGACGGATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGCGGCGATGaa                                                                                      >  1:4018536/1‑93 (MQ=255)
        acTGATGACGCTGGCAGAGAAATTTGACGTTGAGTACGACGGATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGCGGCGATGAAGa                                                                                    >  2:2143856/1‑93 (MQ=255)
        acTGATGACGCTGGCAGAGAAATTTGACGTTGAGTACGACGGATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGCGGCGATGAAGa                                                                                    <  1:5428958/93‑1 (MQ=255)
         cTGATGACGCTGGCAGAGAAATTTGACGTTGAGTACGACGGATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGCGGCGATGAAGAt                                                                                   >  2:3222999/1‑93 (MQ=255)
          tgatgaCGCTGGCAGAGAAATTTGACGTTGAGTACGACGGATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGCGGCGATGAAGAtt                                                                                  <  1:6064188/93‑1 (MQ=255)
            atgaCGCTGGCAGAGAAATTTGACGTTGAGTACGACGGATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGCGGCGATGAAGAtttt                                                                                <  1:4620992/93‑1 (MQ=255)
             tgaCGCTGGCAGAGAAATTTGACGTTGAGTACGACGGATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGCGGCGATGAAGATTTTg                                                                               <  2:6107081/93‑1 (MQ=255)
              gaCGCTGGCAGAGAAATTTGACGTTGAGTACGACGGATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGCGACGATGAAGATTTTGt                                                                              >  1:2639121/1‑93 (MQ=255)
                  cTGGCAGAGAAATTTGACGTTGAGTACGACGGATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGCGGCGATGAAGATTTTGTCGAt                                                                          <  2:1426206/93‑1 (MQ=255)
                   tGGCAGAGAAATTTGACGTTGAGTACGACGGATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGCGGCGATGAAGATTTTGTCGATg                                                                         >  1:7104752/1‑93 (MQ=255)
                   tGGCAGAGAAATTTGACGTTGAGTACGACGGATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGCGGCGATGAAGATTTTGTCGATg                                                                         <  1:3612287/93‑1 (MQ=255)
                   tGGCAGAGAAATTTGACGTTGAGTACGACGGATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGCGGCGATGAAGATTTTGTCGATg                                                                         >  1:5497501/1‑93 (MQ=255)
                    ggCAGAGAAATTTGACGTTGAGTACGACGGATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATTGCGGCGATGAAGATTTTGTCGATGa                                                                        <  1:7443468/93‑1 (MQ=255)
                    ggCAGAGAAATTTGACGTTGAGTACGACGGATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGCGGCGATGAAGATTTTGTCGATGa                                                                        <  1:2845418/93‑1 (MQ=255)
                     gCAGAGAAATTTGACGTTGAGTACGACGGATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGCGGCGATGAAGATTTTGTCGATGaa                                                                       <  2:2006146/93‑1 (MQ=255)
                     gCAGAGAAATTTGACGTTGAGTACGACGGATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGCGGCGATGAAGATTTTGTCGATGaa                                                                       <  1:1939091/93‑1 (MQ=255)
                       agagAAATTTGACGTTGAGTACGACGGATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGCGGCGATGAAGATTTTGTCGATGAAGa                                                                     <  1:5877035/93‑1 (MQ=255)
                        gagaAATTTGACGTTGAGTACGACGGATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGCGGCGATGAAGATTTTGTCGATGAAGAc                                                                    <  1:2504232/93‑1 (MQ=255)
                        gagaAATTTGACGTTGAGTACGACGGATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGCGGCGATGAAGATTTTGTCGATGAAGAc                                                                    <  1:6647791/93‑1 (MQ=255)
                        gagaAATTTGACGTTGAGTACGACGGATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGCGGCGATGAAGATTTTGTCGATGAAGAc                                                                    <  2:589689/93‑1 (MQ=255)
                        gagaAATTTGACGTTGAGTACGACGGATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGCGGCGATGAAGATTTTGTCGATGAAGAc                                                                    >  1:5298268/1‑93 (MQ=255)
                        gagaAATTTGACGTTGAGTACGACGGATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGCGGCGATGAAGATTTTGTCGATGAAGAc                                                                    >  2:4543523/1‑93 (MQ=255)
                          gaAATTTGACGTTGAGTACGACGGATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGCGGCGATGAAGATTTTGTCGATGAAGACGa                                                                  <  1:115961/93‑1 (MQ=255)
                          gaAATTTGACGTTGAGTACGACGGATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGCGGCGATGAAGATTTTGTCGATGAAGACGa                                                                  <  2:4828097/93‑1 (MQ=255)
                           aaaTTTGACGTTGAGTACGACGGATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGCGGCGATGAAGATTTTGTCGATGAAGACGAt                                                                 <  1:7229457/93‑1 (MQ=255)
                           aaaTTTGACGTTGAGTACGACGGATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGCGGCGATGAAGATTTTGTCGATGAAGACGAt                                                                 <  1:3760384/93‑1 (MQ=255)
                           aaaTTTGACGTTGAGTACGACGGATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGCGGCGATGAAGATTTTGTCGATGAAGACGAt                                                                 <  1:2001330/93‑1 (MQ=255)
                            aaTTTGACGTTGAGTACGACGGATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGCGACGATGAAGATTTTGTCGATGAAGACGATg                                                                >  1:2390944/1‑93 (MQ=255)
                             aTTTGACGTTGAGTACGACGGATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGCGGCGATGAAGATTTTGTCGATGAAGACGATGa                                                               <  2:5251632/93‑1 (MQ=255)
                             aTTTGACGTTGAGTACGACGGATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGCGGCGATGAAGATTTTGTCGATGAAGACGATGa                                                               <  2:3532948/93‑1 (MQ=255)
                              tttGACGTTGAGTACGACGGATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGCGGCGATGAAGATTTTGTCGATGAAGACGATGAc                                                              >  2:5177158/1‑93 (MQ=255)
                                tGACGTTGAGTACGACGGATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGCGGCGATGAAGATTTTGTCGATGAAGACGATGACgg                                                            >  2:6218415/1‑93 (MQ=255)
                                 gACGTTGAGTACGACGGATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGCGGCGATGAAGATTTTGTCGATGAAGACGATGACGGa                                                           <  1:6848165/93‑1 (MQ=255)
                                  aCGTTGAGTACGACGGATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGCGGCGATGAAGATTTTGTCGATGAAGACGATGACGGAg                                                          >  1:5809497/1‑93 (MQ=255)
                                  aCGTTGAGTACGACGGATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGCGGCGATGAAGATTTTGTCGATGAAGACGATGACGGAg                                                          <  2:7107727/93‑1 (MQ=255)
                                   cGTTGAGTACGACGGATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGCGGCGATGAAGATTTTGTCGATGAAGACGATGACGGAGt                                                         >  2:1105453/1‑93 (MQ=255)
                                   cGTTGAGTACGACGGATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGCGACGATGAAGATTTTGTCGATGAAGACGATGACGGAGt                                                         >  1:1473635/1‑93 (MQ=255)
                                    gTTGAGTACGACGGATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGCGGCGATGAAGATTTTGTCGATGAAGACGATGACGGAGtt                                                        >  1:5564536/1‑93 (MQ=255)
                                        aGTACGACGGATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGCGACGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGcc                                                    <  2:768597/93‑1 (MQ=255)
                                         gTACGACGGATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGCGGCGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCa                                                   <  2:2849974/93‑1 (MQ=255)
                                          tACGACGGATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGCGGCGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCAc                                                  <  2:915805/93‑1 (MQ=255)
                                          tACGACGGATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGCGACGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCAc                                                  <  1:1895080/93‑1 (MQ=255)
                                            cgacgGATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGCGACGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCACTa                                                >  2:1550120/1‑93 (MQ=255)
                                               cgGATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGCGGCGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCACTAAtt                                             >  1:5668211/1‑93 (MQ=255)
                                                ggATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGCGGCGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCACTAATTa                                            <  2:1867503/93‑1 (MQ=255)
                                                ggATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGCGGCGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCACTAATTa                                            >  1:6823167/1‑93 (MQ=255)
                                                 gATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGCGGCGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCACTAATTaa                                           <  2:7252177/93‑1 (MQ=255)
                                                 gATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGCGGCGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCACTAATTaa                                           <  2:1633922/93‑1 (MQ=255)
                                                 gATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGCGGCGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCACTAATTaa                                           <  2:6562369/93‑1 (MQ=255)
                                                  aTGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGCGGCGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCACTAATTAAg                                          <  1:4502701/93‑1 (MQ=255)
                                                   tGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGCGGCGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCACTAATTAAGc                                         <  2:6019663/93‑1 (MQ=255)
                                                   tGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGCGACGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCACTAATTAAGc                                         >  2:5394872/1‑93 (MQ=255)
                                                      ggCACTTACTTTGAAGATCCTAACGGCGAAGATGGCGACGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCACTAATTAAGCGTa                                      >  2:3960585/1‑93 (MQ=255)
                                                      ggCACTTACTTTGAAGATCCTAACGGCGAAGATGGCGACGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCACTAATTAAGCGTa                                      >  2:3715420/1‑93 (MQ=255)
                                                       gCACTTACTTTGAAGATCCTAACGGCGAAGATGGCGGCGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCACTAATTAAGCGTAg                                     >  2:5730260/1‑93 (MQ=255)
                                                         acttacttTGAAGATCCTAACGGCGAAGATGGCGGCGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCACTAATTAAGCGTAgcg                                   >  2:1508971/1‑93 (MQ=255)
                                                         acttacttTGAAGATCCTAACGGCGAAGATGGCGGCGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCACTAATTAAGCGTAgcg                                   >  2:6805661/1‑93 (MQ=255)
                                                          cttacttTGAAGATCCTAACGGCGAAGATGGCGGCGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCACTAATTAAGCGTAgcgc                                  >  1:4776083/1‑93 (MQ=255)
                                                          cttacttTGAAGATCCTAACGGCGAAGATGGCGGCGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCACTAATTAAGCGTAgcgc                                  >  1:3051244/1‑93 (MQ=255)
                                                             acttTGAAGATCCTAACGGCGAAGATGGCGGCGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCACTAATTAAGCGTAGCGCATc                               <  1:3105937/93‑1 (MQ=255)
                                                              cttTGAAGATCCTAACGGCGAAGATGGCGGCGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCACTAATTAAGCGTAGCGCATCa                              <  2:6036320/93‑1 (MQ=255)
                                                              cttTGAAGATCCTAACGGCGAAGATGGCGGCGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCACTAATTAAGCGTAGCGCATCa                              <  2:2675525/93‑1 (MQ=255)
                                                               tttGAAGATCCTAACGGCGAAGATGGCGACGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCACTAATTAAGCGTAGCGCATCAg                             <  2:4483842/93‑1 (MQ=255)
                                                                 tGAAGATCCTAACGGCGAAGATGGCGGCGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCACTAATTAAGCGTAGCGCATCAGGc                           <  2:4971309/93‑1 (MQ=255)
                                                                  gAAGATCCTAACGGCGAAGATGGCGGCGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCACTAATTAAGCGTAGCGCATCAGGcc                          >  1:2900542/1‑93 (MQ=255)
                                                                  gAAGATCCTAACGGCGAAGATGGCGGCGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCACTAATTAAGCGTAGCGCATCAGGcc                          >  1:3498592/1‑93 (MQ=255)
                                                                  gAAGATCCTAACGGCGAAGATGGCGGCGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCACTAATTAAGCGTAGCGCATCAGGcc                          <  2:6299250/93‑1 (MQ=255)
                                                                    aGATCCTAACGGCGAAGATGGCGGCGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCACTAATTAAGCGTAGCGCATCAGGCCCa                        <  2:2482155/93‑1 (MQ=255)
                                                                    aGATCCTAACGGCGAAGATGGCGGCGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCACTAATTAAGCGTAGCGCATCAGGCCCa                        <  2:2228587/93‑1 (MQ=255)
                                                                     gATCCTAACGGCGAAGATGGCGGCGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCACTAATTAAGCGTAGCGCATCAGGCCCAg                       <  2:526886/93‑1 (MQ=255)
                                                                      aTCCTAACGGCGAAGATGGCGGCGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCACTAATTAAGCGTAGCGCATCAGGCCCAgg                      <  2:135392/93‑1 (MQ=255)
                                                                       tCCTAACGGCGAAGATGGCGGCGATGAAGATTTTGTCGATGAAGACTATGACGGAGTTCGCCACTAATTAAGCGTAGCGCATCAGGCCCAGGt                     >  1:3218076/1‑93 (MQ=255)
                                                                       tCCTAACGGCGAAGATGGCGACGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCACTAATTAAGCGTAGCGCATCAGGCCCAGGt                     >  2:6930998/1‑93 (MQ=255)
                                                                        ccTAACGGCGAAGATGGCGACGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCACTAATTAAGCGTAGCGCATCAGGCCCAGGTa                    >  2:2407129/1‑93 (MQ=255)
                                                                         cTAACGGCGAAGATGGCGGCGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCACTAATTAAGCGTAGCGCATCAGGCCCAGGTAg                   >  1:7172085/1‑93 (MQ=255)
                                                                         cTAACGGCGAAGATGGCGGCGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCACTAATTAAGCGTAGCGCATCAGGCCCAGGTAg                   <  2:3415082/93‑1 (MQ=255)
                                                                         cTAACGGCGAAGATGGCGGCGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCACTAATTAAGCGTAGCGCATCAGGCCCAGGTAg                   >  1:2946563/1‑93 (MQ=255)
                                                                          tAACGGCGAAGATGGCGGCGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCACTAATTAAGCGTAGCGCATCAGGCCCAGGTAGt                  >  1:389165/1‑93 (MQ=255)
                                                                          tAACGGCGAAGATGGCGACGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCACTAATTAAGCGTAGCGCATCAGGCCCAGGTAGt                  <  1:3415118/93‑1 (MQ=255)
                                                                           aaCGGCGAAGATGGCGACGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCACTAATTAAGCGTAGCGCATCAGGCCCAGGTAGtt                 >  1:2365243/1‑93 (MQ=255)
                                                                            aCGGCGAAGATGGCGGCGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCACTAATTAAGCGTAGCGCATCAGGCCCAGGTAGTTc                <  2:5650609/93‑1 (MQ=255)
                                                                               gCGAAGATGGCGGCGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCACTAATTAAGCGTAGCGCATCAGGCCCAGGTAGTTCCTg             >  2:3151228/1‑93 (MQ=255)
                                                                               gCGAAGATGGCGACGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCACTAATTAAGCGTAGCGCATCAGGCCCAGGTAGTTCCTg             >  1:2112387/1‑93 (MQ=255)
                                                                                cGAAGATGGCGGCGATGAAGATTTTGTCGATGAAGACGATGACGGAGGTCGCCACTAATTAAGCGTAGCGCATCAGGCCCAGGTAGTTCCTGc            >  1:4997752/1‑93 (MQ=255)
                                                                                   aGATGGCGGCGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCACTAATTAAGCGTAGCGCATCAGGCCCAGGTAGTTCCTGCAAc         <  1:6250716/93‑1 (MQ=255)
                                                                                   aGATGGCGGCGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCACTAATTAAGCGTAGCGCATCAGGCCCAGGTAGTTCCTGCAAc         <  1:6409690/93‑1 (MQ=255)
                                                                                    gATGGCGGCGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCACTAATTAAGCGTAGCGCATCAGGCCCAGGTAGTTCCTGCAAcc        <  2:6822896/93‑1 (MQ=255)
                                                                                       ggcggcGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCACTAATTAAGCGTAGCGCATCAGGCCCAGGTAGTTCCTGCAACCTgg     <  1:5222178/93‑1 (MQ=255)
                                                                                       ggcggcGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCACTAATTAAGCGTAGCGCATCAGGCCCAGGTAGTTCCTGCAACCTgg     >  1:3338974/1‑93 (MQ=255)
                                                                                       ggCGACGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCACTAATTAAGCGTAGCGCATCAGGCCCAGGTAGTTCCTGCAACCTgg     >  2:1661533/1‑93 (MQ=255)
                                                                                        gcggcgATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCACTAATTAAGCGTAGCGCATCAGGCCCAGGTAGTTCCTGCAACCTGGt    <  2:5147418/93‑1 (MQ=255)
                                                                                          gacgaTGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCACTAATTAAGCGTAGCGCATCAGGCCCAGGTAGTTCCTGCAACCTGGTGa  <  1:6717636/93‑1 (MQ=255)
                                                                                           |                                                                                           
GTTGAACAACTGATGACGCTGGCAGAGAAATTTGACGTTGAGTACGACGGATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGCGACGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCACTAATTAAGCGTAGCGCATCAGGCCCAGGTAGTTCCTGCAACCTGGTGA  >  NC_000913/4476315‑4476497

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AGGTTGAACAACTGATGACGCTGGCAGAGAAATTTGACGTTGAGTACGACGGATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGC‑GACGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCACTAATTAAGCGTAGCGCATCAGGCCCAGGTAGTTCCTGCAACCTGGTGAA  >  NC_000913/4476313‑4476498
                                                                                              |                                                                                            
AGGTTGAACAACTGATGACGCTGGCAGAGAAATTTGACGTTGAGTACGACGGATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGC‑GG                                                                                              >  SRR4302542.305744/1‑94 (MQ=60)
AGGTTGAACAACTGATGACGCTGGCAGAGAAATTTGACGTTGAGTACGACGGATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGC‑GG                                                                                              >  SRR4302542.2877374/1‑94 (MQ=60)
 GGTTGAACAACTGATGACGCTGGCAGAGAAATTTGACGTTGAGTACGACGGATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGC‑GGC                                                                                             <  SRR4302542.3932389/94‑1 (MQ=60)
 GGTTGAACAACTGATGACGCTGGCAGAGAAATTTGACGTTGAGTACGACGGATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGC‑GGC                                                                                             >  SRR4302542.7234071/1‑94 (MQ=60)
  GTTGAACAACTGATGACGCTGGCAGAGAAATTTGACGTTGAGTACGACGGATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGC‑GGCG                                                                                            >  SRR4302542.6360532/1‑94 (MQ=60)
  GTTGAACAACTGATGACGCTGGCAGAGAAATTTGACGTTGAGTACGACGGATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGC‑GACG                                                                                            <  SRR4302542.4544391/94‑1 (MQ=60)
   TTGAACAACTGATGACGCTGGCAGAGAAATTTGACGTTGAGTACGACGGATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGC‑GGCGA                                                                                           >  SRR4302542.3082701/1‑94 (MQ=60)
    TGAACAACTGATGACGCTGGCAGAGAAATTTGACGTTGAGTACGACGGATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGC‑GGCGAT                                                                                          >  SRR4302542.1278673/1‑94 (MQ=60)
    TGAACAACTGATGACGCTGGCAGAGAAATTTGACGTTGAGTACGACGGATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGC‑GGCGAT                                                                                          >  SRR4302542.3385287/1‑94 (MQ=60)
     GAACAACTGATGACGCTGGCAGAGAAATTTGACGTTGAGTACGACGGATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGC‑GGCGATG                                                                                         <  SRR4302542.245608/94‑1 (MQ=60)
     GAACAACTGATGACGCTGGCAGAGAAATTTGACGTTGAGTACGACGGATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGC‑GGCGATG                                                                                         <  SRR4302542.4044699/94‑1 (MQ=60)
      AACAACTGATGACGCTGGCAGAGAAATTTGACGTTGAGTACGACGGATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGC‑GACGATGA                                                                                        <  SRR4302542.6745853/94‑1 (MQ=60)
      AACAACTGATGACGCTGGCAGAGAAATTTGACGTTGAGTACGACGGATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGC‑GACGATGA                                                                                        <  SRR4302542.7042421/94‑1 (MQ=60)
       ACAACTGATGACGCTGGCAGAGAAATTTGACGTTGAGTACGACGGATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGC‑GGCGATGAA                                                                                       <  SRR4302542.2226806/94‑1 (MQ=60)
       ACAACTGATGACGCTGGCAGAGAAATTTGACGTTGAGTACGACGGATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGC‑GGCGATGAA                                                                                       >  SRR4302542.4058193/1‑94 (MQ=60)
       ACAACTGATGACGCTGGCAGAGAAATTTGACGTTGAGTACGACGGATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGC‑GGCGATGAA                                                                                       <  SRR4302542.2069034/94‑1 (MQ=60)
          ACTGATGACGCTGGCAGAGAAATTTGACGTTGAGTACGACGGATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGC‑GGCGATGAAGAT                                                                                    <  SRR4302542.5481712/94‑1 (MQ=60)
           CTGATGACGCTGGCAGAGAAATTTGACGTTGAGTACGACGGATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGC‑GGCGATGAAGATT                                                                                   >  SRR4302542.5282002/1‑94 (MQ=60)
            TGATGACGCTGGCAGAGAAATTTGACGTTGAGTACGACGGATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGC‑GGCGATGAAGATTT                                                                                  <  SRR4302542.6122925/94‑1 (MQ=60)
            TGATGACGCTGGCAGAGAAATTTGACGTTGAGTACGACGGATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGC‑GGCGATGAAGATTT                                                                                  >  SRR4302542.6718828/1‑94 (MQ=60)
             GATGACGCTGGCAGAGAAATTTGACGTTGAGTACGACGGATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGC‑GGCGATGAAGATTTT                                                                                 >  SRR4302542.7141376/1‑94 (MQ=60)
             GATGACGCTGGCAGAGAAATTTGACGTTGAGTACGACGGATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGC‑GGCGATGAAGATTTT                                                                                 >  SRR4302542.3952363/1‑94 (MQ=60)
             GATGACGCTGGCAGAGAAATTTGACGTTGAGTACGACGGATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGC‑GACGATGAAGATTTT                                                                                 >  SRR4302542.6132927/1‑94 (MQ=60)
             GATGACGCTGGCAGAGAAATTTGACGTTGAGTACGACGGATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGC‑GACGATGAAGATTTT                                                                                 <  SRR4302542.2915693/94‑1 (MQ=60)
              ATGACGCTGGCAGAGAAATTTGACGTTGAGTACGACGGATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGC‑GGCGATGAAGATTTTG                                                                                <  SRR4302542.4666137/94‑1 (MQ=60)
              ATGACGCTGGCAGAGAAATTTGACATTGAGTACGACGGATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGC‑GGCGATGAAGATTTTG                                                                                >  SRR4302542.5889821/1‑94 (MQ=60)
               TGACGCTGGCAGAGAAATTTGACGTTGAGTACGACGGATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGC‑GACGATGAAGATTTTGT                                                                               >  SRR4302542.2664609/1‑94 (MQ=60)
                GACGCTGGCAGAGAAATTTGACGTTGAGTACGACGGATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGC‑GGCGATGAAGATTTTGTC                                                                              <  SRR4302542.5682295/94‑1 (MQ=60)
                  CGCTGGCAGAGAAATTTGACGTTGAGTACGACGGATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGCGGGCGATGAAGATTTTGTCG                                                                             >  SRR4302542.2388282/1‑94 (MQ=60)
                   GCTGGCAGAGAAATTTGACGTTGAGTACGACGGATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGC‑GGCGATGAAGATTTTGTCGAT                                                                           <  SRR4302542.325884/94‑1 (MQ=60)
                    CTGGCAGAGAAATTTGACGTTGAGTACGACGGATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGC‑GGCGATGAAGATTTTGTCGATG                                                                          >  SRR4302542.7174492/1‑94 (MQ=60)
                    CTGGCAGAGAAATTTGACGTTGAGTACGACGGATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGC‑GGCGATGAAGATTTTGTCGATG                                                                          >  SRR4302542.5550898/1‑94 (MQ=60)
                    CTGGCAGAGAAATTTGACGTTGAGTACGACGGATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGC‑GACGATGAAGATTTTGTCGATG                                                                          >  SRR4302542.5968176/1‑94 (MQ=60)
                     TGGCAGAGAAATTTGACGTTGAGTACGACGGATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGC‑GGCGATGAAGATTTTGTCGATGA                                                                         <  SRR4302542.3645951/94‑1 (MQ=60)
                      GGCAGAGAAATTTGACGTTGAGTACGACGGATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATTGC‑GGCGATGAAGATTTTGTCGATGAA                                                                        <  SRR4302542.7516349/94‑1 (MQ=60)
                      GGCAGAGAAATTTGACGTTGAGTACGACGGATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGC‑GGCGATGAAGATTTTGTCGATGAA                                                                        <  SRR4302542.2872763/94‑1 (MQ=60)
                       GCAGAGAAATTTGACGTTGAGTACGACGGATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGC‑GGCGATGAAGATTTTGTCGATGAAG                                                                       <  SRR4302542.1955861/94‑1 (MQ=60)
                        CAGAGAAATTTGACGTTGAGTACGACGGATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGC‑GGCGATGAAGATTTTGTCGATGAAGA                                                                      >  SRR4302542.1574894/1‑94 (MQ=60)
                         AGAGAAATTTGACGTTGAGTACGACGGATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGC‑GGCGATGAAGATTTTGTCGATGAAGAC                                                                     <  SRR4302542.5934009/94‑1 (MQ=60)
                         AGAGAAATTTGACGTTGAGTACGACGGATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGC‑GGCGATGAAGATTTTGTCGATGAAGAC                                                                     >  SRR4302542.5349752/1‑94 (MQ=60)
                          GAGAAATTTGACGTTGAGTACGACGGATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGC‑GGCGATGAAGATTTTGTCGATGAAGACG                                                                    <  SRR4302542.2528544/94‑1 (MQ=60)
                          GAGAAATTTGACGTTGAGTACGACGGATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGC‑GGCGATGAAGATTTTGTCGATGAAGACG                                                                    <  SRR4302542.6235507/94‑1 (MQ=60)
                          GAGAAATTTGACGTTGAGTACGACGGATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGC‑GGCGATGAAGATTTTGTCGATGAAGACG                                                                    <  SRR4302542.6713309/94‑1 (MQ=60)
                          GAGAAATTTGACGTTGAGTACGACGGATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGAAGGC‑GACGATGAAGATTTTGTCGATGAAGACG                                                                    >  SRR4302542.1299950/1‑94 (MQ=60)
                            GAAATTTGACGTTGAGTACGACGGATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGC‑GGCGATGAAGATTTTGTCGATGAAGACGAT                                                                  <  SRR4302542.116918/94‑1 (MQ=60)
                             AAATTTGACGTTGAGTACGACGGATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGC‑GGCGATGAAGATTTTGTCGATGAAGACGATG                                                                 <  SRR4302542.2018611/94‑1 (MQ=60)
                             AAATTTGACGTTGAGTACGACGGATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGC‑GGCGATGAAGATTTTGTCGATGAAGACGATG                                                                 >  SRR4302542.1859952/1‑94 (MQ=60)
                             AAATTTGACGTTGAGTACGACGGATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGC‑GGCGATGAAGATTTTGTCGATGAAGACGATG                                                                 <  SRR4302542.3797584/94‑1 (MQ=60)
                             AAATTTGACGTTGAGTACGACGGATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGC‑GGCGATGAAGATTTTGTCGATGAAGACGATG                                                                 <  SRR4302542.7300377/94‑1 (MQ=60)
                             AAATTTGACGTTGAGTACGACGGATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGC‑GACGATGAAGATTTTGTCGATGAAGACGATG                                                                 <  SRR4302542.4912256/94‑1 (MQ=60)
                             AAATTTGACGTTGAGTACGACGGATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGC‑GACGATGAAGATTTTGTCGATGAAGACGATG                                                                 >  SRR4302542.2413580/1‑94 (MQ=60)
                              AATTTGACGTTGAGTACGACGGATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGC‑GGCGATGAAGATTTTGTCGATGAAGACGATGA                                                                >  SRR4302542.4439282/1‑94 (MQ=60)
                                  TGACGTTGAGTACGACGGATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGC‑GGCGATGAAGATTTTGTCGATGAAGACGATGACGGA                                                            <  SRR4302542.1916463/94‑1 (MQ=60)
                                  TGACGTTGAGTACGACGGATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGC‑GACGATGAAGATTTTGTCGATGAAGACGATGACGGA                                                            >  SRR4302542.7002602/1‑94 (MQ=60)
                                   GACGTTGAGTACGACGGATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGC‑GGCGATGAAGATTTTGTCGATGAAGACGATGACGGAG                                                           >  SRR4302542.5865872/1‑94 (MQ=60)
                                   GACGTTGAGTACGACGGATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGC‑GGCGATGAAGATTTTGTCGATGAAGACGATGACGGAG                                                           <  SRR4302542.6915426/94‑1 (MQ=60)
                                   GACGTTGAGTACGACGGATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGC‑GGCGATGAAGATTTTGTCGATGAAGACGATGACGGAG                                                           <  SRR4302542.800716/94‑1 (MQ=60)
                                    ACGTTGAGTACGACGGATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGC‑GACGATGAAGATTTTGTCGATGAAGACGATGACGGAGT                                                          >  SRR4302542.1486356/1‑94 (MQ=60)
                                     CGTTGAGTACGACGGATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGC‑GGCGATGAAGATTTTGTCGATGAAGACGATGACGGAGTT                                                         >  SRR4302542.5618602/1‑94 (MQ=60)
                                      GTTGAGTACGACGGATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGC‑GGCGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTC                                                        >  SRR4302542.3193379/1‑94 (MQ=60)
                                       TTGGGTACGACGGATGGGGCCCTTCCTTTGAAGATCCTAACGGCGAAGATGGC‑GGCGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCG                                                       <  SRR4302542.1221479/94‑1 (MQ=60)
                                       TTGAGTACGACGGATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGC‑GACGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCG                                                       <  SRR4302542.6907208/94‑1 (MQ=60)
                                        TGAGTACGACGGATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGC‑GGCGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGC                                                      >  SRR4302542.2930814/1‑94 (MQ=60)
                                         GAGTACGACGGATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGC‑GGCGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCC                                                     >  SRR4302542.3530215/1‑94 (MQ=60)
                                         GAGTACGACGGATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGC‑GGCGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCC                                                     <  SRR4302542.5831360/94‑1 (MQ=60)
                                          AGTACGACGGATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGC‑GGCGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCA                                                    <  SRR4302542.1369451/94‑1 (MQ=60)
                                            TACGACGGATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGC‑GGCGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCACT                                                  >  SRR4302542.5482640/1‑94 (MQ=60)
                                            TACGACGGATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGC‑GACGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCACT                                                  <  SRR4302542.1911482/94‑1 (MQ=60)
                                             ACGACGGATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGC‑GACGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCACTA                                                 <  SRR4302542.2267634/94‑1 (MQ=60)
                                                ACGGATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGC‑GGCGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCACTAATT                                              >  SRR4302542.5723265/1‑94 (MQ=60)
                                                 CGGATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGC‑GGCGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCACTAATTA                                             <  SRR4302542.2394459/94‑1 (MQ=60)
                                                 CGGATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGC‑GGCGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCACTAATTA                                             >  SRR4302542.6890234/1‑94 (MQ=60)
                                                 CGGATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGC‑GACGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCACTAATTA                                             <  SRR4302542.7263041/94‑1 (MQ=60)
                                                  GGATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGC‑GGCGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCACTAATTAA                                            >  SRR4302542.3058829/1‑94 (MQ=60)
                                                   GATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGC‑GGCGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCACTAATTAAG                                           >  SRR4302542.2046547/1‑94 (MQ=60)
                                                    ATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGC‑GGCGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCACTAATTAAGC                                          <  SRR4302542.725766/94‑1 (MQ=60)
                                                    ATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGC‑GGCGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCACTAATTAAGC                                          <  SRR4302542.3406363/94‑1 (MQ=60)
                                                    ATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGC‑GGCGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCACTAATTAAGC                                          <  SRR4302542.4546851/94‑1 (MQ=60)
                                                     TGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGC‑GGCGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCACTAATTAAGCG                                         >  SRR4302542.5528030/1‑94 (MQ=60)
                                                     TGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGC‑GGCGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCACTAATTAAGCG                                         >  SRR4302542.2562571/1‑94 (MQ=60)
                                                      GGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGC‑GGCGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCACTAATTAAGCGT                                        >  SRR4302542.319501/1‑94 (MQ=60)
                                                      GGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGC‑GGCGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCACTAATTAAGCGT                                        >  SRR4302542.454833/1‑94 (MQ=60)
                                                       GGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGC‑GGCGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCACTAATTAAGCGTA                                       <  SRR4302542.5480566/94‑1 (MQ=60)
                                                        GGCACTTACTTTGAAGATCCTAACGGCGAAGATGGC‑GGCGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCACTAATTAAGCGTAG                                      <  SRR4302542.3634221/94‑1 (MQ=60)
                                                          CACTTACTTTGAAGATCCTAACGGCGAAGATGGC‑GGCGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCACTAATTAAGCGTAGCG                                    >  SRR4302542.373784/1‑94 (MQ=60)
                                                          CACTTACTTTGAAGATCCTAACGGCGAAGATGGC‑GGCGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCACTAATTAAGCGTAGCG                                    >  SRR4302542.7437778/1‑94 (MQ=60)
                                                          CACTTACTTTGAAGATCCTAACGGCGAAGATGGC‑GACGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCACTAATTAAGCGTAGCG                                    >  SRR4302542.2577590/1‑94 (MQ=60)
                                                           ACTTACTTTGAAGATCCTAACGGCGAAGATGGC‑GGCGATTAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCACTAATTAAGCGTAGCGC                                   >  SRR4302542.4740543/1‑94 (MQ=60)
                                                           ACTTACTTTGAAGATCCTAACGGCGAAGATGGC‑GGCGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCACTAATTAAGCGTAGCGC                                   >  SRR4302542.3080367/1‑94 (MQ=60)
                                                           ACTTACTTTGAAGATCCTAACGGCGAAGATGGC‑GGCGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCACTAATTAAGCGTAGCGC                                   >  SRR4302542.3556354/1‑94 (MQ=60)
                                                           ACTTACTTTGAAGATCCTAACGGCGAAGATGGC‑GGCGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCACTAATTAAGCGTAGCGC                                   >  SRR4302542.4822635/1‑94 (MQ=60)
                                                            CTTACTTTGAAGATCCTAACGGCGAAGATGGC‑GACGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCACTAATTAAGCGTAGCGCA                                  >  SRR4302542.2370768/1‑94 (MQ=60)
                                                             TTACTTTGAAGATCCTAACGGCGAAGATGGC‑GGCGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCACTAATTAAGCGTAGCGCAT                                 <  SRR4302542.5817687/94‑1 (MQ=60)
                                                             TTACTTTGAAGATCCTAACGGCGAAGATGGC‑GGCGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCACTAATTAAGCGTAGCGCAT                                 >  SRR4302542.4850717/1‑94 (MQ=60)
                                                               ACTTTGAAGATCCTAACGGCGAAGATGGC‑GGCGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCACTAATTAAGCGTAGCGCATCA                               >  SRR4302542.7453669/1‑94 (MQ=60)
                                                               ACTTTGAAGATCCTAACGGCGAAGATGGC‑GGCGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCACTAATTAAGCGTAGCGCATCA                               <  SRR4302542.3135461/94‑1 (MQ=60)
                                                                CTTTGAAGATCCTAACGGCGAAGATGGC‑GGCGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCACTAATTAAGCGTAGCGCATCAG                              <  SRR4302542.593033/94‑1 (MQ=60)
                                                                 TTTGAAGATCCTAACGGCGAAGATGGC‑GGCGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCACTAATTAAGCGTAGCGCATCAGG                             >  SRR4302542.2018196/1‑94 (MQ=60)
                                                                 TTTGAAGATCCTAACGGCGAAGATGGC‑GGCGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCACTAATTAAGCGTAGCGCATCAGG                             <  SRR4302542.5607723/94‑1 (MQ=60)
                                                                 TTTGAAGATCCTAACGGCGAAGATGGC‑GGCGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCACTAATTAAGCGTAGCGCATCAGG                             <  SRR4302542.6873507/94‑1 (MQ=60)
                                                                   TGAAGATCCTAACGGCGAAGATGGC‑GGCGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCACTAATTAAGCGTAGCGCATCAGGCC                           >  SRR4302542.2928382/1‑94 (MQ=60)
                                                                   TGAAGATCCTAACGGCGAAGATGGC‑GGCGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCACTAATTAAGCGTAGCGCATCAGGCC                           >  SRR4302542.3531316/1‑94 (MQ=60)
                                                                   TGAAGATCCTAACGGCGAAGATGGC‑GACGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCACTAATTAAGCGTAGCGCATCAGGCC                           >  SRR4302542.2169999/1‑94 (MQ=60)
                                                                    GAAGATCCTAACGGCGAAGATGGC‑GGCGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCACTAATTAAGCGTAGCGCATCAGGCCC                          >  SRR4302542.6820252/1‑94 (MQ=60)
                                                                     AAGATCCTAACGGCGAAGATGGC‑GGCGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCACTAATTAAGCGTAGCGCATCAGGCCCA                         >  SRR4302542.196626/1‑94 (MQ=60)
                                                                       GATCCTAACGGCGAAGATGGC‑GACGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCACTAATTAAGCGTAGCGCATCAGGCCCCGG                       >  SRR4302542.6485984/1‑94 (MQ=60)
                                                                        ATCCTAACGGCGAAGATGGC‑GGCGATGAAGATTTTGTCGATGAAGACTATGACGGAGTTCGCCACTAATTAAGCGTAGCGCATCAGGCCCAGGT                      >  SRR4302542.3248538/1‑94 (MQ=60)
                                                                        ATCCTAACGGCGAAGATGGC‑GGCGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCACTAATTAAGCGTAGCGCATCAGGCCCAGGT                      >  SRR4302542.3276154/1‑94 (MQ=60)
                                                                        ATCCTAACGGCGAAGATGGC‑GGCGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCACTAATTAAGCGTAGCGCATCAGGCCCAGGT                      <  SRR4302542.7028028/94‑1 (MQ=60)
                                                                        ATCCTAACGGCGAAGATGGC‑GGCGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCACTAATTAAGCGTAGCGCATCAGGCCCAGGT                      <  SRR4302542.710852/94‑1 (MQ=60)
                                                                        ATCCTAACGGCGAAGATGGC‑GACGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCACTAATTAAGCGTAGCGCATCAGGCCCAGGT                      >  SRR4302542.5232073/1‑94 (MQ=60)
                                                                          CCTAACGGCGAAGATGGC‑GGCGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCACTAATTAAGCGTAGCGCATCAGGCCCAGGTAG                    >  SRR4302542.2692954/1‑94 (MQ=60)
                                                                          CCTAACGGCGAAGATGGC‑GGCGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCACTAATTAAGCGTAGCGCATCAGGCCCAGGTAG                    >  SRR4302542.7242438/1‑94 (MQ=60)
                                                                          CCTAACGGCGAAGATGGC‑GGCGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCACTAATTAAGCGTAGCGCATCAGGCCCAGGTAG                    >  SRR4302542.2974773/1‑94 (MQ=60)
                                                                          CCTAACGGCGAAGATGGC‑GGCGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCACTAATTAAGCGTAGCGCATCAGGCCCAGGTAG                    <  SRR4302542.4810146/94‑1 (MQ=60)
                                                                           CTAACGGCGAAGATGGC‑GGCGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCACTAATTAAGCGTAGCGCATCAGGCCCAGGTAGT                   >  SRR4302542.392535/1‑94 (MQ=60)
                                                                            TAACGGCGAAGATGGC‑GGCGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCACTAATTAAGCGTAGCGCATCAGGCCCAGGTAGTT                  <  SRR4302542.2146817/94‑1 (MQ=60)
                                                                            TAACGGCGAAGATGGC‑GACGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCACTAATTAAGCGTAGCGCATCAGGCCCAGGTAGTT                  <  SRR4302542.3447117/94‑1 (MQ=60)
                                                                            TAACGGCGAAGATGGC‑GACGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCACTAATTAAGCGTAGCGCATCAGGCCCAGGTAGTT                  >  SRR4302542.3875949/1‑94 (MQ=60)
                                                                            TAACGGCGAAGATGGC‑GACGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCACTAATTAAGCGTAGCGCATCAGGCCCAGGTAGTT                  >  SRR4302542.2385712/1‑94 (MQ=60)
                                                                             AACGGCGAAGATGGC‑GACGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCACTAATTAAGCGTAGCGCATCAGGCCCAGGTAGTTC                 >  SRR4302542.2094271/1‑94 (MQ=60)
                                                                               CGGCGAAGATGGC‑GGCGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCACTAATTAAGCGTAGCGCATCAGGCCCAGGTAGTTCCT               <  SRR4302542.2832615/94‑1 (MQ=60)
                                                                               CGGCGAAGATGGC‑GACGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCACTAATTAAGCGTAGCGCATCAGGCCCAGGTAGTTCCT               >  SRR4302542.543426/1‑94 (MQ=60)
                                                                                GGCGAAGATGGC‑GGCGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCACTAATTAAGCGTAGCGCATCAGGCCCAGGTAGTTCCTG              >  SRR4302542.3719635/1‑94 (MQ=60)
                                                                                GGCGAAGATGGC‑GGCGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCACTAATTAAGCGTAGCGCATCAGGCCCAGGTAGTTCCTG              >  SRR4302542.5205520/1‑94 (MQ=60)
                                                                                GGCGAAGATGGC‑GACGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCACTAATTAAGCGTAGCGCATCAGGCCCAGGTAGTTCCTG              >  SRR4302542.2130621/1‑94 (MQ=60)
                                                                                 GCGAAGATGGC‑GGCGATGAAGATTTTGTCGATGAAGACGATGACGGAGGTCGCCACTAATTAAGCGTAGCGCATCAGGCCCAGGTAGTTCCTGC             >  SRR4302542.5046303/1‑94 (MQ=60)
                                                                                  CGAAGATGGC‑GGCGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCACTAATTAAGCGTAGCGCATCAGGCCCAGGTAGTTCCTGCA            >  SRR4302542.5713924/1‑94 (MQ=60)
                                                                                    AAGATGGC‑GGCGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCACTAATTAAGCGTAGCGCATCAGGCCCAGGTAGTTCCTGCAAC          <  SRR4302542.7230602/94‑1 (MQ=60)
                                                                                     AGATGGC‑GGCGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCACTAATTAAGCGTAGCGCATCAGGCCCAGGTAGTTCCTGCAACC         <  SRR4302542.6312308/94‑1 (MQ=60)
                                                                                     AGATGGC‑GGCGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCACTAATTAAGCGTAGCGCATCAGGCCCAGGTAGTTCCTGCAACC         <  SRR4302542.6396795/94‑1 (MQ=60)
                                                                                     AGATGGC‑GGCGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCACTAATTAAGCGTAGCGCATCAGGCCCAGGTAGTTCCTGCAACC         <  SRR4302542.6472854/94‑1 (MQ=60)
                                                                                        TGGC‑GGCGATGAAGATTTTGTTGATGAAGACGATGACGGAGTTCGCCACTAATTAAGCGTAGCGCATCAGGCCCAGGTAGTTCCTGCAACCTGG      <  SRR4302542.2959588/94‑1 (MQ=60)
                                                                                        TGGC‑GGCGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCACTAATTAAGCGTAGCGCATCAGGCCCAGGTAGTTCCTGCAACCTGG      <  SRR4302542.3034864/94‑1 (MQ=60)
                                                                                        TGGC‑GGCGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCACTAATTAAGCGTAGCGCATCAGGCCCAGGTAGTTCCTGCAACCTGG      >  SRR4302542.3370399/1‑94 (MQ=60)
                                                                                         GGC‑GGCGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCACTAATTAAGCGTAGCGCATCAGGCCCAGGTAGTTCCTGCAACCTGGT     <  SRR4302542.5272966/94‑1 (MQ=60)
                                                                                         GGC‑GGCGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCACTAATTAAGCGTAGCGCATCAGGCCCAGGTAGTTCCTGCAACCTGGT     >  SRR4302542.205316/1‑94 (MQ=60)
                                                                                           C‑GGCGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCACTAATTAAGCGTAGCGCATCAGGCCCAGGTAGTTCCTGCAACCTGGTGA   >  SRR4302542.5492823/1‑94 (MQ=60)
                                                                                           C‑GGCGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCACTAATTAAGCGTAGCGCATCAGGCCCAGGTAGTTCCTGCAACCTGGTGA   <  SRR4302542.6084198/94‑1 (MQ=60)
                                                                                             GGCGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCACTAATTAAGCGTAGCGCATCAGGCCCAGGTAGTTCCTGCAACCTGGTGAA  <  SRR4302542.218259/94‑1 (MQ=60)
                                                                                             GACGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCACTAATTAAGCGTAGCGCATCAGGCCCAGGTAGTTCCTGCAACCTGGTGAA  <  SRR4302542.6783795/94‑1 (MQ=60)
                                                                                              |                                                                                            
AGGTTGAACAACTGATGACGCTGGCAGAGAAATTTGACGTTGAGTACGACGGATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGC‑GACGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCACTAATTAAGCGTAGCGCATCAGGCCCAGGTAGTTCCTGCAACCTGGTGAA  >  NC_000913/4476313‑4476498

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑