Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1061 294.1 15065038 98.6% 14854127 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,477,321 Δ1 bp coding (12/1182 nt) yjgN → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,477,3210A.100.0% 987.4 / NA 212coding (12/1182 nt)yjgNhypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (123/89);  total (123/89)

Only 100 of 212 total aligned reads displayed.

AACATTTTACTGTCTGTATATATAAGTTATATCAATGGATTTTTTAATTTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGATAGTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTTGTTAACGATTATAACG  >  NC_000913/4477232‑4477408
                                                                                         |                                                                                       
 aCATTTTACTGTCTGTATATATAAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCg                                                                                   >  1:7200218/1‑93 (MQ=255)
 aCATTTTACTGTCTGTATATATAAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCg                                                                                   >  1:4149335/1‑93 (MQ=255)
   aTTTTACTGTCTGTATATATAAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTc                                                                                 >  2:1976076/1‑93 (MQ=255)
    ttttACTGTCTGTATATATAAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTcc                                                                                >  2:6619854/1‑93 (MQ=255)
    ttttACTGTCTGTATATATAAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTcc                                                                                >  2:2104770/1‑93 (MQ=255)
    ttttACTGTCTGTATATATAAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTcc                                                                                <  1:5137975/93‑1 (MQ=255)
     tttACTGTCTGTATATATAAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTccc                                                                               >  1:545263/1‑93 (MQ=255)
      ttACTGTCTGTATATATAAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCa                                                                              >  2:1305174/1‑93 (MQ=255)
       tACTGTCTGTATATATAAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCAt                                                                             <  2:3407331/93‑1 (MQ=255)
            tctgtATATATAAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCgtt                                                                        <  2:5277628/93‑1 (MQ=255)
            tctgtATATATAAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCgtt                                                                        <  2:2479502/93‑1 (MQ=255)
              tgtATATATAAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCgtttg                                                                      >  1:1267332/1‑93 (MQ=255)
              tgtATATATAAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCgtttg                                                                      <  2:925306/93‑1 (MQ=255)
              tgtATATATAAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCgtttg                                                                      <  2:2249704/93‑1 (MQ=255)
              tgtATATATAAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCgtttg                                                                      >  1:6528692/1‑93 (MQ=255)
               gtATATATAAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCgtttgt                                                                     <  2:2962929/93‑1 (MQ=255)
                tatatataAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCgtttgtt                                                                    <  1:7091516/93‑1 (MQ=255)
                 atatataAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCgtttgttt                                                                   <  1:6233728/93‑1 (MQ=255)
                 atatataAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCgtttgttt                                                                   >  2:530088/1‑93 (MQ=255)
                 atatataAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCgtttgttt                                                                   <  1:5741378/93‑1 (MQ=255)
                  tatataAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGtttt                                                                  >  2:3574380/1‑93 (MQ=255)
                  tatataAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGtttt                                                                  <  1:3566586/93‑1 (MQ=255)
                  tatataAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGtttt                                                                  >  2:4138484/1‑93 (MQ=255)
                   atataAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGttttt                                                                 >  1:3370963/1‑93 (MQ=255)
                    tataAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTc                                                                >  1:6327080/1‑93 (MQ=255)
                    tataAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTc                                                                >  2:457013/1‑93 (MQ=255)
                      taAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCAt                                                              <  2:2309082/93‑1 (MQ=255)
                      taAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCAt                                                              >  1:2508556/1‑93 (MQ=255)
                      taAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCAt                                                              >  1:5548570/1‑93 (MQ=255)
                       aaGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATg                                                             <  1:3622377/93‑1 (MQ=255)
                        aGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATgg                                                            <  1:4798873/93‑1 (MQ=255)
                         gTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGt                                                           >  2:4009392/1‑93 (MQ=255)
                          ttATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTa                                                          >  1:1507482/1‑93 (MQ=255)
                           tataTCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTAc                                                         >  1:1299451/1‑93 (MQ=255)
                           tataTCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTAc                                                         >  1:4205524/1‑93 (MQ=255)
                            atatCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACa                                                        >  1:6099663/1‑93 (MQ=255)
                             tatCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAg                                                       <  1:397448/93‑1 (MQ=255)
                             tatCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAg                                                       >  1:7262329/1‑93 (MQ=255)
                              atCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAgg                                                      >  2:4327794/1‑93 (MQ=255)
                              atCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAgg                                                      >  2:1066864/1‑93 (MQ=255)
                               tCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGt                                                     <  1:4392858/93‑1 (MQ=255)
                                 aaTGGATTTTTTAA‑TTCAGGGAATTTTTTTGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTga                                                   >  1:2925086/1‑93 (MQ=255)
                                 aaTGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTga                                                   >  2:5469700/1‑93 (MQ=255)
                                  aTGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTgag                                                  <  1:2384855/93‑1 (MQ=255)
                                   tGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTgaga                                                 >  1:4878587/1‑93 (MQ=255)
                                     gATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTgagaga                                               >  2:35006/1‑93 (MQ=255)
                                      aTTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGat                                              <  2:1481134/93‑1 (MQ=255)
                                        tttttAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGatat                                            <  2:417448/93‑1 (MQ=255)
                                          tttAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATAttt                                          <  2:1592448/93‑1 (MQ=255)
                                             taattCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTc                                        <  1:670334/90‑1 (MQ=255)
                                             taattCAGGGAATTTTTATGGCTCAAGATATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTc                                        >  1:286637/4‑93 (MQ=255)
                                              aattCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCt                                       >  1:2105301/3‑93 (MQ=255)
                                              aattCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCt                                       <  2:854563/91‑1 (MQ=255)
                                                ttCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTa                                     <  2:3537106/93‑1 (MQ=255)
                                                 tCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTAt                                    <  2:6518345/93‑1 (MQ=255)
                                                 tCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTAt                                    >  2:6164519/1‑93 (MQ=255)
                                                  cAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTAtt                                   <  2:3188963/93‑1 (MQ=255)
                                                   aGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTAttt                                  <  2:2159920/93‑1 (MQ=255)
                                                    gggAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTtg                                 >  1:916451/1‑93 (MQ=255)
                                                    gggAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTtg                                 >  2:6842077/1‑93 (MQ=255)
                                                      gAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTtgtg                               <  2:4740183/93‑1 (MQ=255)
                                                      gAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTtgng                               >  2:7399716/1‑93 (MQ=255)
                                                       aaTTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTtgtgt                              <  1:1962916/93‑1 (MQ=255)
                                                        aTTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTtgtgtg                             >  1:628455/1‑93 (MQ=255)
                                                         tttttATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTgtgg                            >  2:5739741/1‑93 (MQ=255)
                                                             tATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGaa                        <  1:1140456/93‑1 (MQ=255)
                                                               tGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAAtg                      >  2:5805143/1‑93 (MQ=255)
                                                               tGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAAtg                      >  1:3517261/1‑93 (MQ=255)
                                                                ggCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAAtgt                     <  1:265013/93‑1 (MQ=255)
                                                                 gCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAAtgtg                    >  2:5908954/1‑93 (MQ=255)
                                                                 gCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAAtgtg                    >  1:316405/1‑93 (MQ=255)
                                                                  cTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATgtgt                   <  1:7498861/93‑1 (MQ=255)
                                                                   tCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGtgtt                  <  2:7087292/93‑1 (MQ=255)
                                                                     aaGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATCTGTGTGGTGAATGtgttgt                <  1:5392361/93‑1 (MQ=255)
                                                                       gTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTTGTTa              >  2:3329601/1‑93 (MQ=255)
                                                                       gTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTTGTTa              <  2:6892067/93‑1 (MQ=255)
                                                                        ttattaATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTTGTTaa             >  2:6636054/1‑93 (MQ=255)
                                                                        ttattaATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTTGTTaa             >  1:3928840/1‑93 (MQ=255)
                                                                        ttattaATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTTGTTaa             >  1:5943669/1‑93 (MQ=255)
                                                                        ttattaATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTTGTTaa             <  2:3003361/93‑1 (MQ=255)
                                                                         tattaATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTTGTTAAc            <  1:1401544/93‑1 (MQ=255)
                                                                         tattaATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTTGTTAAc            >  2:1053749/1‑93 (MQ=255)
                                                                         tattaATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTTGTTAAc            >  2:3583808/1‑93 (MQ=255)
                                                                         tattaATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTTGTTAAc            >  2:2802262/1‑93 (MQ=255)
                                                                         tattaATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTTGTTAAc            >  1:3022754/1‑93 (MQ=255)
                                                                          attaATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTTGTTAACg           >  2:6913372/1‑93 (MQ=255)
                                                                          attaATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTTGTTAACg           >  2:744281/1‑93 (MQ=255)
                                                                           ttaATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTTGTTAACGa          >  1:1800268/1‑93 (MQ=255)
                                                                              aTGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTTGTTAACGATta       <  1:6365385/93‑1 (MQ=255)
                                                                              aTGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTTGTTAACGATta       <  1:6044536/93‑1 (MQ=255)
                                                                               tGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTTGTTAACGATtat      >  2:2750703/1‑93 (MQ=255)
                                                                                gAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTTGTTAACGATtata     <  1:6075946/93‑1 (MQ=255)
                                                                                gAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTTGTTAACGATtata     >  2:2906588/1‑93 (MQ=255)
                                                                                 aaaTGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTTGTTAACGATTATaa    <  1:6919874/93‑1 (MQ=255)
                                                                                 aaaTGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTTGTTAACGATTATaa    <  2:1773886/93‑1 (MQ=255)
                                                                                 aaaTGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTTGTTAACGATTATaa    <  1:2278884/93‑1 (MQ=255)
                                                                                  aaTGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTTGTTAACGATTATAAc   <  1:4736184/93‑1 (MQ=255)
                                                                                  aaTGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTTGTTAACGATTATAAc   >  2:3885213/1‑93 (MQ=255)
                                                                                  aaTGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGCACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTTGTTAACGATTATAAc   <  1:2783162/93‑1 (MQ=255)
                                                                                   aTGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTTGTTAACGATTATAACg  >  2:4831213/1‑93 (MQ=255)
                                                                                         |                                                                                       
AACATTTTACTGTCTGTATATATAAGTTATATCAATGGATTTTTTAATTTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGATAGTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTTGTTAACGATTATAACG  >  NC_000913/4477232‑4477408

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GGCAGAACATTTTACTGTCTGTATATATAAGTTATATCAATGGATTTTTTAATTTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGATAGTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTTGTTAACGATTATAACGCTAGGT  >  NC_000913/4477227‑4477414
                                                                                              |                                                                                             
GGCAGAACATTTTACTGTCTGTATATATAAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGATG                                                                                               <  SRR4302542.3416504/94‑1 (MQ=60)
GGCAGAACATTTTACTGTCTGTATATATAAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGATG                                                                                               <  SRR4302542.4032781/94‑1 (MQ=60)
  CAGAACATTTTACTGTCTGTATATATAAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTTAAGTTATTAATGAAATGGAT‑GTT                                                                                            >  SRR4302542.3618694/1‑94 (MQ=60)
  CAGAACATTTTACTGTCTGTATATATAAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTT                                                                                            >  SRR4302542.1663043/1‑94 (MQ=60)
   AGAACATTTTACTGTCTGTATATATAAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTC                                                                                           >  SRR4302542.3320341/1‑94 (MQ=60)
    GAACATTTTACTGTCTGTATATATAAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCC                                                                                          >  SRR4302542.6053074/1‑94 (MQ=60)
     AACATTTTACTGTCTGTATATATAAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCG                                                                                         >  SRR4302542.7270876/1‑94 (MQ=60)
     AACATTTTACTGTCTGTATATATAAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCG                                                                                         >  SRR4302542.4190206/1‑94 (MQ=60)
      ACATTTTACTGTCTGTATATATAAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGT                                                                                        >  SRR4302542.1187045/1‑94 (MQ=60)
      ACATTTTACTGTCTGTATATATAAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGT                                                                                        <  SRR4302542.5922406/94‑1 (MQ=60)
      ACATTTTACTGTCTGTATATATAAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGT                                                                                        <  SRR4302542.936973/94‑1 (MQ=60)
       CATTTTACTGTCTGTATATATAAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTC                                                                                       >  SRR4302542.3178537/1‑94 (MQ=60)
        ATTTTACTGTCTGTATATATAAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCC                                                                                      >  SRR4302542.1744178/1‑94 (MQ=60)
        ATTTTACTGTCTGTATATATAAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCC                                                                                      >  SRR4302542.2722146/1‑94 (MQ=60)
         TTTTACTGTCTGTATATATAAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCC                                                                                     >  SRR4302542.549973/1‑94 (MQ=60)
         TTTTACTGTCTGTATATATAAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCC                                                                                     <  SRR4302542.5187915/94‑1 (MQ=60)
                GTCTGTATATATAAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTT                                                                              >  SRR4302542.6983923/1‑94 (MQ=60)
                 TCTGTATATATAAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTT                                                                             <  SRR4302542.6124313/94‑1 (MQ=60)
                  CTGTATATATAAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTG                                                                            >  SRR4302542.6593038/1‑94 (MQ=60)
                  CTGTATATATAAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTG                                                                            >  SRR4302542.1278222/1‑94 (MQ=60)
                     TATATATAAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTT                                                                         <  SRR4302542.7161087/94‑1 (MQ=60)
                      ATATATAAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTT                                                                        >  SRR4302542.3296949/1‑94 (MQ=60)
                      ATATATAAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTT                                                                        <  SRR4302542.6295109/94‑1 (MQ=60)
                      ATATATAAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTT                                                                        <  SRR4302542.5797116/94‑1 (MQ=60)
                       TATATAAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTT                                                                       >  SRR4302542.3402611/1‑94 (MQ=60)
                       TATATAAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTT                                                                       <  SRR4302542.3599881/94‑1 (MQ=60)
                        ATATAAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTC                                                                      >  SRR4302542.1950651/1‑94 (MQ=60)
                        ATATAAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTC                                                                      >  SRR4302542.6389425/1‑94 (MQ=60)
                         TATAAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCA                                                                     >  SRR4302542.2387816/1‑94 (MQ=60)
                          ATAAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCAT                                                                    >  SRR4302542.5602486/1‑94 (MQ=60)
                          ATAAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCAT                                                                    >  SRR4302542.2532898/1‑94 (MQ=60)
                            AAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGG                                                                  <  SRR4302542.3656122/94‑1 (MQ=60)
                             AGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGT                                                                 <  SRR4302542.4845621/94‑1 (MQ=60)
                             AGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGT                                                                 >  SRR4302542.5359791/1‑94 (MQ=60)
                              GTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTA                                                                <  SRR4302542.3594293/94‑1 (MQ=60)
                              GTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTA                                                                >  SRR4302542.1381332/1‑94 (MQ=60)
                              GTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTA                                                                >  SRR4302542.1520517/1‑94 (MQ=60)
                               TTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTAC                                                               >  SRR4302542.4246989/1‑94 (MQ=60)
                               TTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTAC                                                               >  SRR4302542.1310642/1‑94 (MQ=60)
                                TATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACA                                                              >  SRR4302542.6158693/1‑94 (MQ=60)
                                 ATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAG                                                             >  SRR4302542.7333525/1‑94 (MQ=60)
                                  TATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGG                                                            <  SRR4302542.400884/94‑1 (MQ=60)
                                  TATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGG                                                            >  SRR4302542.5606725/1‑94 (MQ=60)
                                   ATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGT                                                           <  SRR4302542.6332022/94‑1 (MQ=60)
                                   ATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGT                                                           >  SRR4302542.2101873/1‑94 (MQ=60)
                                   ATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGT                                                           >  SRR4302542.698617/1‑94 (MQ=60)
                                    TCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTG                                                          <  SRR4302542.4242162/94‑1 (MQ=60)
                                    TCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTG                                                          <  SRR4302542.4435957/94‑1 (MQ=60)
                                     CAATGGATTTTTTAA‑TTCAGGGAATTTTTTTGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGA                                                         >  SRR4302542.2953109/1‑94 (MQ=60)
                                      AATGGATTTTTTAA‑TTCAGGGAATTTTTAAGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAG                                                        <  SRR4302542.2363417/94‑1 (MQ=60)
                                       ATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGA                                                       >  SRR4302542.4926065/1‑94 (MQ=60)
                                       ATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGA                                                       <  SRR4302542.2406940/94‑1 (MQ=60)
                                        TGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAG                                                      <  SRR4302542.2533202/94‑1 (MQ=60)
                                         GGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGA                                                     <  SRR4302542.5495145/94‑1 (MQ=60)
                                          GATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGAT                                                    >  SRR4302542.6285701/1‑94 (MQ=60)
                                               TTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTT                                               <  SRR4302542.2931308/94‑1 (MQ=60)
                                                TTAA‑TTCAGGGAATTTTTATGGCTCAAGATATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTC                                              >  SRR4302542.289139/1‑94 (MQ=60)
                                                 TAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCT                                             >  SRR4302542.6138535/1‑94 (MQ=60)
                                                 TAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCT                                             <  SRR4302542.676119/94‑1 (MQ=60)
                                                 TAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCT                                             >  SRR4302542.2123468/1‑94 (MQ=60)
                                                   AATTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTT                                            <  SRR4302542.4051254/94‑1 (MQ=60)
                                                    ATTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTA                                           >  SRR4302542.4261894/1‑94 (MQ=60)
                                                    ATTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTA                                           >  SRR4302542.3509723/1‑94 (MQ=60)
                                                     TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTAT                                          >  SRR4302542.5518628/1‑94 (MQ=60)
                                                       CAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTT                                        <  SRR4302542.2810108/94‑1 (MQ=60)
                                                        GGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGAACAGGGGAGAGATATTTTCTTATTTG                                       >  SRR4302542.3717704/1‑94 (MQ=60)
                                                        AGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGTGAGATATTTTCTTCTTTG                                       >  SRR4302542.592105/1‑94 (MQ=60)
                                                        AGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTG                                       >  SRR4302542.924354/1‑94 (MQ=60)
                                                        AGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTG                                       <  SRR4302542.5125898/94‑1 (MQ=60)
                                                          GGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTG                                     >  SRR4302542.6499791/1‑94 (MQ=60)
                                                            AATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTG                                   <  SRR4302542.1979886/94‑1 (MQ=60)
                                                            AATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTG                                   >  SRR4302542.633856/1‑94 (MQ=60)
                                                            AATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTG                                   >  SRR4302542.1507872/1‑94 (MQ=60)
                                                            AATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTG                                   <  SRR4302542.7326975/94‑1 (MQ=60)
                                                             ATTTTTATGGCTCAAGTTATTAATGAAATGGGT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGG                                  >  SRR4302542.481897/1‑94 (MQ=60)
                                                             ATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGG                                  >  SRR4302542.6697345/1‑94 (MQ=60)
                                                             ATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGG                                  >  SRR4302542.6349338/1‑94 (MQ=60)
                                                                  TATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAAT                             <  SRR4302542.4924064/94‑1 (MQ=60)
                                                                  TATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAAT                             <  SRR4302542.1150219/94‑1 (MQ=60)
                                                                   ATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATG                            >  SRR4302542.3550145/1‑94 (MQ=60)
                                                                    TGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGT                           >  SRR4302542.6415802/1‑94 (MQ=60)
                                                                     GGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTG                          >  SRR4302542.319159/1‑94 (MQ=60)
                                                                     GGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTG                          <  SRR4302542.267272/94‑1 (MQ=60)
                                                                      GCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGT                         >  SRR4302542.876459/1‑94 (MQ=60)
                                                                      GCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGT                         <  SRR4302542.1305627/94‑1 (MQ=60)
                                                                       CTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTT                        <  SRR4302542.962865/94‑1 (MQ=60)
                                                                       CTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTT                        <  SRR4302542.7572589/94‑1 (MQ=60)
                                                                        TCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTTG                       >  SRR4302542.4409301/1‑94 (MQ=60)
                                                                          AAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTTGTT                     <  SRR4302542.240592/94‑1 (MQ=60)
                                                                          AAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTTGTT                     <  SRR4302542.3040340/94‑1 (MQ=60)
                                                                          AAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATCTGTGTGGTGAATGTGTTGTT                     <  SRR4302542.5444739/94‑1 (MQ=60)
                                                                           NNTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTTGTTA                    <  SRR4302542.338805/94‑1 (MQ=60)
                                                                            GTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTTGTTAA                   >  SRR4302542.6001243/1‑94 (MQ=60)
                                                                            GTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTTGTTAA                   >  SRR4302542.3967605/1‑94 (MQ=60)
                                                                             TTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTTGTTAAC                  >  SRR4302542.3051612/1‑94 (MQ=60)
                                                                              TATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTTGTTAACG                 <  SRR4302542.1413661/94‑1 (MQ=60)
                                                                               ATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTTGTTAACGA                >  SRR4302542.1815879/1‑94 (MQ=60)
                                                                                  AATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTTGTTAACGATTA             >  SRR4302542.4663840/1‑94 (MQ=60)
                                                                                  AATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTTGTTAACGATTA             <  SRR4302542.3645927/94‑1 (MQ=60)
                                                                                  AATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTTGTTAACGATTA             >  SRR4302542.652668/1‑94 (MQ=60)
                                                                                   ATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTTGTTAACGATTAT            <  SRR4302542.2221969/94‑1 (MQ=60)
                                                                                   ATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTTGTTAACGATTAT            <  SRR4302542.6428070/94‑1 (MQ=60)
                                                                                   ATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTTGTTAACGATTAT            <  SRR4302542.6103041/94‑1 (MQ=60)
                                                                                     GAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTTGTTAACGATTATAA          <  SRR4302542.6134790/94‑1 (MQ=60)
                                                                                     GAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTTGTTAACGATTATAA          >  SRR4302542.4013405/1‑94 (MQ=60)
                                                                                      AAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTTGTTAACGATTATAAC         <  SRR4302542.2298474/94‑1 (MQ=60)
                                                                                      AAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTTGTTAACGATTATAAC         <  SRR4302542.6987813/94‑1 (MQ=60)
                                                                                      AAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTTGTTAACGATTATAAC         >  SRR4302542.754456/1‑94 (MQ=60)
                                                                                       AATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTTGTTAACGATTATAACG        <  SRR4302542.7217115/94‑1 (MQ=60)
                                                                                       AATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTTGTTAACGATTATAACG        <  SRR4302542.4782368/94‑1 (MQ=60)
                                                                                       AATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGCACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTTGTTAACGATTATAACG        <  SRR4302542.2809962/94‑1 (MQ=60)
                                                                                        ATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTTGTTAACGATTATAACGC       >  SRR4302542.3240020/1‑94 (MQ=60)
                                                                                        ATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTTGTTAACGATTATAACGC       >  SRR4302542.5439561/1‑94 (MQ=60)
                                                                                         TGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTTGTTAACGATTATAACGCT      >  SRR4302542.4467502/1‑94 (MQ=60)
                                                                                          GGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTTGTTAACGATTATAACGCTA     <  SRR4302542.5493017/94‑1 (MQ=60)
                                                                                          GGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTTGTTAACGATTATAACGCTA     >  SRR4302542.2015255/1‑94 (MQ=60)
                                                                                           GAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTTGTTAACGATTATAACGCTAG    <  SRR4302542.5799194/94‑1 (MQ=60)
                                                                                           GAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTTGTTAACGATTATAACGCTAG    <  SRR4302542.409175/94‑1 (MQ=60)
                                                                                              TGTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTTGTTAACGATTATAACGCTAGGT  >  SRR4302542.2344805/1‑94 (MQ=60)
                                                                                              TGTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTAGTGAATGTGTTGTTAACGATTATAACGCTAGGT  >  SRR4302542.5365473/1‑94 (MQ=60)
                                                                                              |                                                                                             
GGCAGAACATTTTACTGTCTGTATATATAAGTTATATCAATGGATTTTTTAATTTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGATAGTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTTGTTAACGATTATAACGCTAGGT  >  NC_000913/4477227‑4477414

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑